[{"supplementarymaterial":"yes","OA_place":"publisher","title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","oa":1,"citation":{"ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2026;61(2):356-371.e12. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, vol. 61, no. 2, Elsevier, 2026, p. 356–371.e12, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>, vol. 61, no. 2. Elsevier, p. 356–371.e12, 2026.","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12."},"date_updated":"2026-07-23T06:27:15Z","PlanS_conform":"1","scopus_import":"1","file_date_updated":"2026-07-23T06:26:25Z","_id":"20859","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"2","day":"11","dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","publication_status":"published","pmid":1,"author":[{"full_name":"Company-Garrido, Iván","first_name":"Iván","last_name":"Company-Garrido"},{"first_name":"Alberto","last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto"},{"first_name":"Mariona","last_name":"Colomer-Rosell","full_name":"Colomer-Rosell, Mariona"},{"first_name":"Bernard","last_name":"Ciraulo","full_name":"Ciraulo, Bernard"},{"last_name":"Molkenbur","first_name":"Ronja","full_name":"Molkenbur, Ronja"},{"full_name":"Lanzerstorfer, Peter","first_name":"Peter","last_name":"Lanzerstorfer"},{"full_name":"Pezzano, Fabio","first_name":"Fabio","last_name":"Pezzano"},{"first_name":"Costanza","last_name":"Agazzi","full_name":"Agazzi, Costanza"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jain, Saumey","first_name":"Saumey","last_name":"Jain"},{"last_name":"Jacques","first_name":"Jeroen M.","full_name":"Jacques, Jeroen M."},{"first_name":"Valeria","last_name":"Venturini","full_name":"Venturini, Valeria"},{"first_name":"Christian","last_name":"Knapp","full_name":"Knapp, Christian"},{"last_name":"Xie","first_name":"Yufei","full_name":"Xie, Yufei"},{"orcid":"0000-0001-5145-4609","first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack"},{"first_name":"Julian","last_name":"Weghuber","full_name":"Weghuber, Julian"},{"last_name":"Schaaf","first_name":"Marcel","full_name":"Schaaf, Marcel"},{"full_name":"Quidant, Romain","last_name":"Quidant","first_name":"Romain"},{"full_name":"Kiermaier, Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0001-6165-5738","last_name":"Kiermaier"},{"last_name":"Ortega Arroyo","first_name":"Jaime","full_name":"Ortega Arroyo, Jaime"},{"full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht"},{"last_name":"Wieser","orcid":"0000-0002-2670-2217","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan"}],"department":[{"_id":"Bio"},{"_id":"NanoFab"}],"date_created":"2025-12-28T23:01:27Z","external_id":{"pmid":["41192429"]},"keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"type":"journal_article","month":"02","page":"356-371.e12","file":[{"file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","date_updated":"2026-07-23T06:26:25Z","date_created":"2026-07-23T06:26:25Z","access_level":"open_access","relation":"main_file","creator":"dernst","file_size":12342817,"content_type":"application/pdf","file_id":"22388","success":1,"checksum":"52fd52d2d19a4514f8fcc1b40f420ca2"}],"article_processing_charge":"Yes (in subscription journal)","language":[{"iso":"eng"}],"abstract":[{"text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo.","lang":"eng"}],"publisher":"Elsevier","das_tickbox":"1","volume":61,"publication":"Developmental Cell","date_published":"2026-02-11T00:00:00Z","intvolume":"        61","OA_type":"hybrid","ddc":["570"],"oa_version":"Published Version","article_type":"original","year":"2026","researchdata_availability":"upon request","doi":"10.1016/j.devcel.2025.10.006","quality_controlled":"1","acknowledged_ssus":[{"_id":"NanoFab"}],"has_accepted_license":"1","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]}},{"article_number":"eadx4047","day":"25","acknowledgement":"We thank L. Pelkmans and D. Dormann for providing Dyrk3-EGFP plasmids; M. Heuzé for providing a RFP-Pericentrin plasmid; T. Balla for providing a PH-Akt-GFP plasmid; E. Snaar-Jagalska for providing a pLenti-V6.3 Ultra-Chili plasmid; T. Tang for providing CEP120 a plasmid; D. Trono for providing pMD2.G and psSPAX2 plasmids; M. Sixt for providing EB3-mCherry and EMTB-mCherry plasmids as well as 3T3 fibroblasts, Lifeact-GFP Hoxb8 cells, and LX293 cells; M. Duggan for RNA isolation from migrating DCs; M. Schuster from the Biomedical Sequencing Facility at CeMM; J. Schwarz for providing Jurkat T cells; M. Götz for initial transcriptome analysis; M. Götz and F. Merino for discussion and sharing reagents; F. Gärtner for discussions and support; M. Benjamin Braun for critical reading of the manuscript; and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.\r\nThis work was supported by Peter Hans Hofschneider Professorship of the Stiftung Experimentelle Biomedizin (J.R.); German Research Foundation grant “CRC914, project A12” (J.R); German Research Foundation grant “SPP2332, project 492014049” (J.R.); LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (J.R.); Medical & Clinician Scientist Program (MCSP) LMU Munich (J.K.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy – EXC2151 – 390873048 (D.B.); Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) Grossgeräteantrag 457838313 and under Germany’s Excellence Strategy – EXC 2151 – 390873048 (E.K.); Ministry of Innovation, Science and Research of North-Rhine-Westphalia (fellowship AZ: 421-8.03.03.02-137069) (E.K.); TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments (E.K.); and CZI grant DAF2020-225401 and grant (DOI https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (R.H.).","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"17","_id":"19663","file_date_updated":"2025-05-12T07:46:10Z","scopus_import":"1","date_updated":"2025-09-30T12:26:21Z","project":[{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy","grant_number":"CZI01"}],"oa":1,"citation":{"apa":"Schmitt, M. T., Kroll, J., Ruiz-Fernandez, M. J. A., Hauschild, R., Ghosh, S., Kameritsch, P., … Renkawitz, J. (2025). Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>","chicago":"Schmitt, Madeleine T., Janina Kroll, Mauricio J.A. Ruiz-Fernandez, Robert Hauschild, Shaunak Ghosh, Petra Kameritsch, Jack Merrin, et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>. AAAS, 2025. <a href=\"https://doi.org/10.1126/sciadv.adx4047\">https://doi.org/10.1126/sciadv.adx4047</a>.","ama":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, et al. Protecting centrosomes from fracturing enables efficient cell navigation. <i>Science Advances</i>. 2025;11(17). doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>","ista":"Schmitt MT, Kroll J, Ruiz-Fernandez MJA, Hauschild R, Ghosh S, Kameritsch P, Merrin J, Schmid J, Stefanowski K, Thomae AW, Cheng J, Öztan GN, Konopka P, Ortega GC, Penz T, Bach L, Baumjohann D, Bock C, Straub T, Meissner F, Kiermaier E, Renkawitz J. 2025. Protecting centrosomes from fracturing enables efficient cell navigation. Science Advances. 11(17), eadx4047.","short":"M.T. Schmitt, J. Kroll, M.J.A. Ruiz-Fernandez, R. Hauschild, S. Ghosh, P. Kameritsch, J. Merrin, J. Schmid, K. Stefanowski, A.W. Thomae, J. Cheng, G.N. Öztan, P. Konopka, G.C. Ortega, T. Penz, L. Bach, D. Baumjohann, C. Bock, T. Straub, F. Meissner, E. Kiermaier, J. Renkawitz, Science Advances 11 (2025).","ieee":"M. T. Schmitt <i>et al.</i>, “Protecting centrosomes from fracturing enables efficient cell navigation,” <i>Science Advances</i>, vol. 11, no. 17. AAAS, 2025.","mla":"Schmitt, Madeleine T., et al. “Protecting Centrosomes from Fracturing Enables Efficient Cell Navigation.” <i>Science Advances</i>, vol. 11, no. 17, eadx4047, AAAS, 2025, doi:<a href=\"https://doi.org/10.1126/sciadv.adx4047\">10.1126/sciadv.adx4047</a>."},"OA_place":"publisher","title":"Protecting centrosomes from fracturing enables efficient cell navigation","isi":1,"type":"journal_article","month":"04","file":[{"success":1,"file_id":"19679","checksum":"e8ba22922fa5b23ccfcce8865f57226c","content_type":"application/pdf","file_size":2707050,"creator":"dernst","relation":"main_file","access_level":"open_access","date_updated":"2025-05-12T07:46:10Z","date_created":"2025-05-12T07:46:10Z","file_name":"2025_ScienceAdvance_Schmitt.pdf"}],"status":"public","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"external_id":{"pmid":["40279414"],"isi":["001476113400016"]},"date_created":"2025-05-11T22:02:38Z","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"author":[{"last_name":"Schmitt","first_name":"Madeleine T.","full_name":"Schmitt, Madeleine T."},{"last_name":"Kroll","first_name":"Janina","full_name":"Kroll, Janina"},{"first_name":"Mauricio J.A.","last_name":"Ruiz-Fernandez","full_name":"Ruiz-Fernandez, Mauricio J.A."},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"last_name":"Ghosh","first_name":"Shaunak","full_name":"Ghosh, Shaunak"},{"last_name":"Kameritsch","first_name":"Petra","full_name":"Kameritsch, Petra"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"full_name":"Schmid, Johanna","last_name":"Schmid","first_name":"Johanna"},{"first_name":"Kasia","last_name":"Stefanowski","full_name":"Stefanowski, Kasia"},{"full_name":"Thomae, Andreas W.","last_name":"Thomae","first_name":"Andreas W."},{"full_name":"Cheng, Jingyuan","first_name":"Jingyuan","last_name":"Cheng"},{"last_name":"Öztan","first_name":"Gamze Naz","full_name":"Öztan, Gamze Naz"},{"full_name":"Konopka, Peter","last_name":"Konopka","first_name":"Peter"},{"full_name":"Ortega, Germán Camargo","first_name":"Germán Camargo","last_name":"Ortega"},{"first_name":"Thomas","last_name":"Penz","full_name":"Penz, Thomas"},{"first_name":"Luisa","last_name":"Bach","full_name":"Bach, Luisa"},{"first_name":"Dirk","last_name":"Baumjohann","full_name":"Baumjohann, Dirk"},{"first_name":"Christoph","last_name":"Bock","full_name":"Bock, Christoph"},{"full_name":"Straub, Tobias","first_name":"Tobias","last_name":"Straub"},{"last_name":"Meissner","first_name":"Felix","full_name":"Meissner, Felix"},{"last_name":"Kiermaier","first_name":"Eva","orcid":"0000-0001-6165-5738","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva"},{"full_name":"Renkawitz, Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz","orcid":"0000-0003-2856-3369","first_name":"Jörg"}],"pmid":1,"publication_status":"published","intvolume":"        11","OA_type":"gold","volume":11,"publication":"Science Advances","date_published":"2025-04-25T00:00:00Z","publisher":"AAAS","abstract":[{"lang":"eng","text":"The centrosome is a microtubule orchestrator, nucleating and anchoring microtubules that grow radially and exert forces on cargos. At the same time, mechanical stresses from the microenvironment and cellular shape changes compress and bend microtubules. Yet, centrosomes are membraneless organelles, raising the question of how centrosomes withstand mechanical forces. Here, we discover that centrosomes can deform and even fracture. We reveal that centrosomes experience deformations during navigational pathfinding within motile cells. Coherence of the centrosome is maintained by Dyrk3 and cNAP1, preventing fracturing by forces. While cells can compensate for the depletion of centriolar-based centrosomes, the fracturing of centrosomes impedes cellular function by generating coexisting microtubule organizing centers that compete during path navigation and thereby cause cellular entanglement in the microenvironment. Our findings show that cells actively maintain the integrity of the centrosome to withstand mechanical forces. These results suggest that centrosome stability preservation is fundamental, given that almost all cells in multicellular organisms experience forces."}],"article_processing_charge":"Yes","language":[{"iso":"eng"}],"DOAJ_listed":"1","publication_identifier":{"eissn":["2375-2548"]},"has_accepted_license":"1","doi":"10.1126/sciadv.adx4047","quality_controlled":"1","year":"2025","article_type":"original","oa_version":"Published Version","ddc":["570"]},{"intvolume":"       219","publication":"The Journal of Cell Biology","date_published":"2020-06-01T00:00:00Z","ec_funded":1,"volume":219,"publisher":"Rockefeller University Press","abstract":[{"lang":"eng","text":"Cells navigating through complex tissues face a fundamental challenge: while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion: (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device: they sense cell shape and control actomyosin retraction to sustain cellular coherence."}],"language":[{"iso":"eng"}],"article_processing_charge":"No","publication_identifier":{"eissn":["1540-8140"]},"has_accepted_license":"1","quality_controlled":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"}],"doi":"10.1083/jcb.201907154","year":"2020","article_type":"original","ddc":["570"],"oa_version":"Published Version","acknowledgement":"The authors thank the Scientific Service Units (Life Sciences, Bioimaging, Preclinical) of the Institute of Science and Technology Austria for excellent support. This work was funded by the European Research Council (ERC StG 281556 and CoG 724373), two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20 to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O. Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734) and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014) co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European Funds for Social and Regional Development.","day":"01","article_number":"e201907154","issue":"6","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"7875","scopus_import":"1","file_date_updated":"2020-11-24T13:25:13Z","date_updated":"2025-04-14T13:10:03Z","oa":1,"citation":{"apa":"Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin, J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>","chicago":"Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>. Rockefeller University Press, 2020. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>.","ama":"Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. 2020;219(6). doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>","short":"A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin, O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt, The Journal of Cell Biology 219 (2020).","ista":"Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 219(6), e201907154.","mla":"Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>, vol. 219, no. 6, e201907154, Rockefeller University Press, 2020, doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>.","ieee":"A. Kopf <i>et al.</i>, “Microtubules control cellular shape and coherence in amoeboid migrating cells,” <i>The Journal of Cell Biology</i>, vol. 219, no. 6. Rockefeller University Press, 2020."},"project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes"},{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373","call_identifier":"H2020","name":"Cellular Navigation Along Spatial Gradients"},{"name":"Mechanical adaptation of lamellipodial actin","call_identifier":"FWF","grant_number":"P29911","_id":"26018E70-B435-11E9-9278-68D0E5697425"},{"grant_number":"W1250-B20","call_identifier":"FWF","name":"Nano-Analytics of Cellular Systems","_id":"252C3B08-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"_id":"25A48D24-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 1396-2014","name":"Molecular and system level view of immune cell migration"}],"title":"Microtubules control cellular shape and coherence in amoeboid migrating cells","isi":1,"corr_author":"1","file":[{"creator":"dernst","success":1,"checksum":"cb0b9c77842ae1214caade7b77e4d82d","file_id":"8801","file_size":7536712,"content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_name":"2020_JCellBiol_Kopf.pdf","date_updated":"2020-11-24T13:25:13Z","date_created":"2020-11-24T13:25:13Z"}],"type":"journal_article","month":"06","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"status":"public","external_id":{"isi":["000538141100020"],"pmid":["32379884"]},"date_created":"2020-05-24T22:00:56Z","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"pmid":1,"author":[{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","first_name":"Aglaja","last_name":"Kopf","full_name":"Kopf, Aglaja"},{"id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz","first_name":"Jörg","orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg"},{"full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild"},{"first_name":"Irute","last_name":"Girkontaite","full_name":"Girkontaite, Irute"},{"last_name":"Tedford","first_name":"Kerry","full_name":"Tedford, Kerry"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack"},{"last_name":"Thorn-Seshold","first_name":"Oliver","full_name":"Thorn-Seshold, Oliver"},{"id":"E8F27F48-3EBA-11E9-92A1-B709E6697425","last_name":"Trauner","first_name":"Dirk","full_name":"Trauner, Dirk"},{"first_name":"Hans","last_name":"Häcker","full_name":"Häcker, Hans"},{"full_name":"Fischer, Klaus Dieter","last_name":"Fischer","first_name":"Klaus Dieter"},{"full_name":"Kiermaier, Eva","last_name":"Kiermaier","first_name":"Eva","orcid":"0000-0001-6165-5738","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K"}],"publication_status":"published"},{"year":"2016","publist_id":"5570","oa_version":"Submitted Version","article_type":"original","doi":"10.1126/science.aad0512","acknowledged_ssus":[{"_id":"SSU"}],"quality_controlled":"1","abstract":[{"lang":"eng","text":"The addition of polysialic acid to N- and/or O-linked glycans, referred to as polysialylation, is a rare posttranslational modification that is mainly known to control the developmental plasticity of the nervous system. Here we show that CCR7, the central chemokine receptor controlling immune cell trafficking to secondary lymphatic organs, carries polysialic acid. This modification is essential for the recognition of the CCR7 ligand CCL21. As a consequence, dendritic cell trafficking is abrogated in polysialyltransferase-deficient mice, manifesting as disturbed lymph node homeostasis and unresponsiveness to inflammatory stimuli. Structure-function analysis of chemokine-receptor interactions reveals that CCL21 adopts an autoinhibited conformation, which is released upon interaction with polysialic acid. Thus, we describe a glycosylation-mediated immune cell trafficking disorder and its mechanistic basis.\r\n"}],"publisher":"American Association for the Advancement of Science","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583642/","open_access":"1"}],"article_processing_charge":"No","language":[{"iso":"eng"}],"ec_funded":1,"volume":351,"date_published":"2016-01-08T00:00:00Z","publication":"Science","intvolume":"       351","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:52:57Z","external_id":{"isi":["000367806500045"],"pmid":["26657283"]},"publication_status":"published","author":[{"id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0001-6165-5738","last_name":"Kiermaier","full_name":"Kiermaier, Eva"},{"full_name":"Moussion, Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87","first_name":"Christine","last_name":"Moussion"},{"first_name":"Christopher","last_name":"Veldkamp","full_name":"Veldkamp, Christopher"},{"last_name":"Gerardy  Schahn","first_name":"Rita","full_name":"Gerardy  Schahn, Rita"},{"first_name":"Ingrid","last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid"},{"full_name":"Williams, Larry","first_name":"Larry","last_name":"Williams"},{"last_name":"Chaffee","first_name":"Gary","full_name":"Chaffee, Gary"},{"full_name":"Phillips, Andrew","first_name":"Andrew","last_name":"Phillips"},{"last_name":"Freiberger","first_name":"Friedrich","full_name":"Freiberger, Friedrich"},{"first_name":"Richard","last_name":"Imre","full_name":"Imre, Richard"},{"first_name":"Deni","last_name":"Taleski","full_name":"Taleski, Deni"},{"last_name":"Payne","first_name":"Richard","full_name":"Payne, Richard"},{"first_name":"Asolina","last_name":"Braun","full_name":"Braun, Asolina"},{"full_name":"Förster, Reinhold","last_name":"Förster","first_name":"Reinhold"},{"full_name":"Mechtler, Karl","first_name":"Karl","last_name":"Mechtler"},{"full_name":"Mühlenhoff, Martina","last_name":"Mühlenhoff","first_name":"Martina"},{"full_name":"Volkman, Brian","last_name":"Volkman","first_name":"Brian"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt"}],"pmid":1,"type":"journal_article","month":"01","page":"186 - 190","status":"public","project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes"},{"grant_number":"289720","name":"Stromal Cell-immune Cell Interactions in Health and Disease","call_identifier":"FP7","_id":"25A76F58-B435-11E9-9278-68D0E5697425"},{"_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12","call_identifier":"FWF","name":"Cytoskeletal force generation and force transduction of migrating leukocytes"}],"citation":{"apa":"Kiermaier, E., Moussion, C., Veldkamp, C., Gerardy  Schahn, R., de Vries, I., Williams, L., … Sixt, M. K. (2016). Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aad0512\">https://doi.org/10.1126/science.aad0512</a>","chicago":"Kiermaier, Eva, Christine Moussion, Christopher Veldkamp, Rita Gerardy  Schahn, Ingrid de Vries, Larry Williams, Gary Chaffee, et al. “Polysialylation Controls Dendritic Cell Trafficking by Regulating Chemokine Recognition.” <i>Science</i>. American Association for the Advancement of Science, 2016. <a href=\"https://doi.org/10.1126/science.aad0512\">https://doi.org/10.1126/science.aad0512</a>.","ama":"Kiermaier E, Moussion C, Veldkamp C, et al. Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. <i>Science</i>. 2016;351(6269):186-190. doi:<a href=\"https://doi.org/10.1126/science.aad0512\">10.1126/science.aad0512</a>","ista":"Kiermaier E, Moussion C, Veldkamp C, Gerardy  Schahn R, de Vries I, Williams L, Chaffee G, Phillips A, Freiberger F, Imre R, Taleski D, Payne R, Braun A, Förster R, Mechtler K, Mühlenhoff M, Volkman B, Sixt MK. 2016. Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. Science. 351(6269), 186–190.","short":"E. Kiermaier, C. Moussion, C. Veldkamp, R. Gerardy  Schahn, I. de Vries, L. Williams, G. Chaffee, A. Phillips, F. Freiberger, R. Imre, D. Taleski, R. Payne, A. Braun, R. Förster, K. Mechtler, M. Mühlenhoff, B. Volkman, M.K. Sixt, Science 351 (2016) 186–190.","ieee":"E. Kiermaier <i>et al.</i>, “Polysialylation controls dendritic cell trafficking by regulating chemokine recognition,” <i>Science</i>, vol. 351, no. 6269. American Association for the Advancement of Science, pp. 186–190, 2016.","mla":"Kiermaier, Eva, et al. “Polysialylation Controls Dendritic Cell Trafficking by Regulating Chemokine Recognition.” <i>Science</i>, vol. 351, no. 6269, American Association for the Advancement of Science, 2016, pp. 186–90, doi:<a href=\"https://doi.org/10.1126/science.aad0512\">10.1126/science.aad0512</a>."},"oa":1,"date_updated":"2025-09-18T11:01:30Z","corr_author":"1","isi":1,"title":"Polysialylation controls dendritic cell trafficking by regulating chemokine recognition","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"6269","day":"08","acknowledgement":"We thank S. Schüchner and E. Ogris for kindly providing the antibody to GFP, M. Helmbrecht and A. Huber for providing Nrp2−/− mice, the IST Scientific Support Facilities for excellent services, and J. Renkawitz and K. Vaahtomeri for critically reading the manuscript. ","scopus_import":"1","_id":"1599"},{"publication_status":"published","pmid":1,"author":[{"full_name":"Veldkamp, Christopher","last_name":"Veldkamp","first_name":"Christopher"},{"orcid":"0000-0001-6165-5738","first_name":"Eva","last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva"},{"last_name":"Gabel Eissens","first_name":"Skylar","full_name":"Gabel Eissens, Skylar"},{"first_name":"Miranda","last_name":"Gillitzer","full_name":"Gillitzer, Miranda"},{"first_name":"David","last_name":"Lippner","full_name":"Lippner, David"},{"full_name":"Disilvio, Frank","last_name":"Disilvio","first_name":"Frank"},{"last_name":"Mueller","first_name":"Casey","full_name":"Mueller, Casey"},{"full_name":"Wantuch, Paeton","last_name":"Wantuch","first_name":"Paeton"},{"first_name":"Gary","last_name":"Chaffee","full_name":"Chaffee, Gary"},{"full_name":"Famiglietti, Michael","first_name":"Michael","last_name":"Famiglietti"},{"full_name":"Zgoba, Danielle","first_name":"Danielle","last_name":"Zgoba"},{"full_name":"Bailey, Asha","last_name":"Bailey","first_name":"Asha"},{"first_name":"Yaya","last_name":"Bah","full_name":"Bah, Yaya"},{"first_name":"Samantha","last_name":"Engebretson","full_name":"Engebretson, Samantha"},{"first_name":"David","last_name":"Graupner","full_name":"Graupner, David"},{"full_name":"Lackner, Emily","last_name":"Lackner","first_name":"Emily"},{"full_name":"Larosa, Vincent","last_name":"Larosa","first_name":"Vincent"},{"last_name":"Medeiros","first_name":"Tysha","full_name":"Medeiros, Tysha"},{"full_name":"Olson, Michael","first_name":"Michael","last_name":"Olson"},{"full_name":"Phillips, Andrew","first_name":"Andrew","last_name":"Phillips"},{"full_name":"Pyles, Harley","first_name":"Harley","last_name":"Pyles"},{"full_name":"Richard, Amanda","first_name":"Amanda","last_name":"Richard"},{"last_name":"Schoeller","first_name":"Scott","full_name":"Schoeller, Scott"},{"last_name":"Touzeau","first_name":"Boris","full_name":"Touzeau, Boris"},{"first_name":"Larry","last_name":"Williams","full_name":"Williams, Larry"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-6620-9179"},{"last_name":"Peterson","first_name":"Francis","full_name":"Peterson, Francis"}],"department":[{"_id":"MiSi"}],"external_id":{"pmid":["26115234"],"isi":["000358105100001"]},"date_created":"2018-12-11T11:53:03Z","status":"public","page":"4163 - 4166","type":"journal_article","month":"06","isi":1,"title":"Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites","citation":{"mla":"Veldkamp, Christopher, et al. “Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.” <i>Biochemistry</i>, vol. 54, no. 27, American Chemical Society, 2015, pp. 4163–66, doi:<a href=\"https://doi.org/10.1021/acs.biochem.5b00560\">10.1021/acs.biochem.5b00560</a>.","ieee":"C. Veldkamp <i>et al.</i>, “Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites,” <i>Biochemistry</i>, vol. 54, no. 27. American Chemical Society, pp. 4163–4166, 2015.","short":"C. Veldkamp, E. Kiermaier, S. Gabel Eissens, M. Gillitzer, D. Lippner, F. Disilvio, C. Mueller, P. Wantuch, G. Chaffee, M. Famiglietti, D. Zgoba, A. Bailey, Y. Bah, S. Engebretson, D. Graupner, E. Lackner, V. Larosa, T. Medeiros, M. Olson, A. Phillips, H. Pyles, A. Richard, S. Schoeller, B. Touzeau, L. Williams, M.K. Sixt, F. Peterson, Biochemistry 54 (2015) 4163–4166.","ista":"Veldkamp C, Kiermaier E, Gabel Eissens S, Gillitzer M, Lippner D, Disilvio F, Mueller C, Wantuch P, Chaffee G, Famiglietti M, Zgoba D, Bailey A, Bah Y, Engebretson S, Graupner D, Lackner E, Larosa V, Medeiros T, Olson M, Phillips A, Pyles H, Richard A, Schoeller S, Touzeau B, Williams L, Sixt MK, Peterson F. 2015. Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites. Biochemistry. 54(27), 4163–4166.","chicago":"Veldkamp, Christopher, Eva Kiermaier, Skylar Gabel Eissens, Miranda Gillitzer, David Lippner, Frank Disilvio, Casey Mueller, et al. “Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.” <i>Biochemistry</i>. American Chemical Society, 2015. <a href=\"https://doi.org/10.1021/acs.biochem.5b00560\">https://doi.org/10.1021/acs.biochem.5b00560</a>.","ama":"Veldkamp C, Kiermaier E, Gabel Eissens S, et al. Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites. <i>Biochemistry</i>. 2015;54(27):4163-4166. doi:<a href=\"https://doi.org/10.1021/acs.biochem.5b00560\">10.1021/acs.biochem.5b00560</a>","apa":"Veldkamp, C., Kiermaier, E., Gabel Eissens, S., Gillitzer, M., Lippner, D., Disilvio, F., … Peterson, F. (2015). Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites. <i>Biochemistry</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.biochem.5b00560\">https://doi.org/10.1021/acs.biochem.5b00560</a>"},"oa":1,"project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7","grant_number":"281556"}],"date_updated":"2025-09-23T10:47:25Z","scopus_import":"1","_id":"1618","issue":"27","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","day":"26","oa_version":"Submitted Version","year":"2015","publist_id":"5548","quality_controlled":"1","doi":"10.1021/acs.biochem.5b00560","language":[{"iso":"eng"}],"article_processing_charge":"No","abstract":[{"lang":"eng","text":"CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed."}],"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809050/","open_access":"1"}],"publisher":"American Chemical Society","publication":"Biochemistry","date_published":"2015-06-26T00:00:00Z","ec_funded":1,"volume":54,"intvolume":"        54"},{"quality_controlled":"1","doi":"10.1126/science.aad0867","oa_version":"None","year":"2015","publist_id":"5459","date_published":"2015-09-04T00:00:00Z","publication":"Science","volume":349,"intvolume":"       349","language":[{"iso":"eng"}],"article_processing_charge":"No","publisher":"American Association for the Advancement of Science","status":"public","page":"1055 - 1056","type":"journal_article","month":"09","publication_status":"published","author":[{"full_name":"Kiermaier, Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738","first_name":"Eva","last_name":"Kiermaier"},{"last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"}],"department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:53:28Z","external_id":{"isi":["000360628900020"]},"scopus_import":"1","_id":"1686","issue":"6252","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","day":"04","corr_author":"1","isi":1,"title":"Fragmented communication between immune cells: Neutrophils blaze a trail with migratory cues for T cells to follow to sites of infection","citation":{"ista":"Kiermaier E, Sixt MK. 2015. Fragmented communication between immune cells: Neutrophils blaze a trail with migratory cues for T cells to follow to sites of infection. Science. 349(6252), 1055–1056.","short":"E. Kiermaier, M.K. Sixt, Science 349 (2015) 1055–1056.","mla":"Kiermaier, Eva, and Michael K. Sixt. “Fragmented Communication between Immune Cells: Neutrophils Blaze a Trail with Migratory Cues for T Cells to Follow to Sites of Infection.” <i>Science</i>, vol. 349, no. 6252, American Association for the Advancement of Science, 2015, pp. 1055–56, doi:<a href=\"https://doi.org/10.1126/science.aad0867\">10.1126/science.aad0867</a>.","ieee":"E. Kiermaier and M. K. Sixt, “Fragmented communication between immune cells: Neutrophils blaze a trail with migratory cues for T cells to follow to sites of infection,” <i>Science</i>, vol. 349, no. 6252. American Association for the Advancement of Science, pp. 1055–1056, 2015.","apa":"Kiermaier, E., &#38; Sixt, M. K. (2015). Fragmented communication between immune cells: Neutrophils blaze a trail with migratory cues for T cells to follow to sites of infection. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aad0867\">https://doi.org/10.1126/science.aad0867</a>","ama":"Kiermaier E, Sixt MK. Fragmented communication between immune cells: Neutrophils blaze a trail with migratory cues for T cells to follow to sites of infection. <i>Science</i>. 2015;349(6252):1055-1056. doi:<a href=\"https://doi.org/10.1126/science.aad0867\">10.1126/science.aad0867</a>","chicago":"Kiermaier, Eva, and Michael K Sixt. “Fragmented Communication between Immune Cells: Neutrophils Blaze a Trail with Migratory Cues for T Cells to Follow to Sites of Infection.” <i>Science</i>. American Association for the Advancement of Science, 2015. <a href=\"https://doi.org/10.1126/science.aad0867\">https://doi.org/10.1126/science.aad0867</a>."},"date_updated":"2025-09-23T08:52:36Z"}]