[{"OA_place":"publisher","date_published":"2023-11-30T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","ddc":["570"],"year":"2023","citation":{"ista":"Hennessey-Wesen M. 2023. Adaptive mutation in E. coli modulated by luxS. Institute of Science and Technology Austria.","ama":"Hennessey-Wesen M. Adaptive mutation in E. coli modulated by luxS. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:14641\">10.15479/at:ista:14641</a>","chicago":"Hennessey-Wesen, Mike. “Adaptive Mutation in E. Coli Modulated by LuxS.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:14641\">https://doi.org/10.15479/at:ista:14641</a>.","short":"M. Hennessey-Wesen, Adaptive Mutation in E. Coli Modulated by LuxS, Institute of Science and Technology Austria, 2023.","ieee":"M. Hennessey-Wesen, “Adaptive mutation in E. coli modulated by luxS,” Institute of Science and Technology Austria, 2023.","mla":"Hennessey-Wesen, Mike. <i>Adaptive Mutation in E. Coli Modulated by LuxS</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:14641\">10.15479/at:ista:14641</a>.","apa":"Hennessey-Wesen, M. (2023). <i>Adaptive mutation in E. coli modulated by luxS</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:14641\">https://doi.org/10.15479/at:ista:14641</a>"},"day":"30","has_accepted_license":"1","abstract":[{"text":"Mutation rates represent the net result of complex interactions among various\r\ncellular processes and can dramatically influence the evolutionary fate of\r\nmicrobial populations. However, many popular techniques used to study\r\nmutations are subject to the confounding effects of heredity and the subtleties\r\nof adaptation to selection, all of which make it difficult to observe any dynamic\r\nresponses of mutation rates to fitness challenges. Furthermore, in spite of the\r\nubiquity of quorum sensing systems across the bacterial domain and relevance\r\nfor many physiological behaviors, the effects of such mechanisms on mutation\r\nrate and adaptation remain poorly understood. In the following work, I\r\npresent the development of a microfluidic droplet-based method to measure\r\nsingle base-pair mutation rates in growing populations of the bacterium\r\nEscherichia coli. I use this method to observe a stress-induced increase in\r\nmutation rate that is mediated by luxS, a highly conserved bacterial quorum\r\nsensing component. I also show that the aforementioned increase in mutation\r\nrate, and its associated control by luxS, corresponds to a higher degree of\r\nadaptability under competitive environments.","lang":"eng"}],"_id":"14641","language":[{"iso":"eng"}],"type":"dissertation","ec_funded":1,"status":"public","page":"104","date_updated":"2026-04-07T13:29:59Z","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"CampIT"}],"file_date_updated":"2025-07-17T11:20:25Z","alternative_title":["ISTA Thesis"],"title":"Adaptive mutation in E. coli modulated by luxS","date_created":"2023-12-04T13:17:37Z","oa_version":"Published Version","keyword":["microfluidics","miceobiology","mutations","quorum sensing"],"publication_identifier":{"issn":["2663-337X"]},"file":[{"access_level":"closed","content_type":"application/vnd.oasis.opendocument.text","date_created":"2023-12-06T13:13:26Z","file_size":46405919,"embargo_to":"open_access","checksum":"4127c285b34f4bf7fb31ef24f9d14c25","file_name":"mike_thesis_v06-12-2023.odt","date_updated":"2024-11-30T23:30:05Z","file_id":"14648","relation":"source_file","creator":"mhenness"},{"embargo":"2026-07-18","checksum":"f5203a61eddaf35235bbc51904d73982","file_name":"mike_thesis_v06-12-2023.pdf","relation":"main_file","creator":"mhenness","file_id":"14649","date_updated":"2025-07-17T11:20:25Z","embargo_to":"open_access","access_level":"closed","content_type":"application/pdf","file_size":21282155,"date_created":"2023-12-06T13:14:15Z"},{"file_name":"2023_Hennessey_Michael_Thesis_print.pdf","checksum":"902102d26d30e74dbd6cdd70a65820c3","creator":"cchlebak","file_id":"19720","date_updated":"2025-05-20T22:31:34Z","relation":"other","embargo_to":"open_access","description":"for printing purposes only","access_level":"closed","date_created":"2025-05-20T12:59:12Z","title":"Print version","file_size":45847968,"content_type":"application/pdf"}],"supervisor":[{"id":"3A374330-F248-11E8-B48F-1D18A9856A87","first_name":"Björn","orcid":"0000-0003-2057-2754","last_name":"Hof","full_name":"Hof, Björn"}],"publication_status":"published","article_processing_charge":"No","corr_author":"1","publisher":"Institute of Science and Technology Austria","author":[{"last_name":"Hennessey-Wesen","full_name":"Hennessey-Wesen, Mike","id":"3F338C72-F248-11E8-B48F-1D18A9856A87","first_name":"Mike"}],"department":[{"_id":"GradSch"},{"_id":"BjHo"}],"month":"11","project":[{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"665385","name":"International IST Doctoral Program"}],"doi":"10.15479/at:ista:14641","degree_awarded":"PhD"},{"status":"public","page":"425","publisher":"IST Austria","article_processing_charge":"No","extern":"1","file_date_updated":"2020-07-22T14:45:07Z","month":"01","author":[{"full_name":"Maslov, Mikhail","last_name":"Maslov","orcid":"0000-0003-4074-2570","first_name":"Mikhail","id":"2E65BB0E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kondrashov","full_name":"Kondrashov, Fyodor","orcid":"0000-0001-8243-4694","id":"44FDEF62-F248-11E8-B48F-1D18A9856A87","first_name":"Fyodor"},{"full_name":"Artner, Christina","last_name":"Artner","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","first_name":"Christina"},{"full_name":"Hennessey-Wesen, Mike","last_name":"Hennessey-Wesen","id":"3F338C72-F248-11E8-B48F-1D18A9856A87","first_name":"Mike"},{"last_name":"Kavcic","full_name":"Kavcic, Bor","first_name":"Bor","id":"350F91D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6041-254X"},{"last_name":"Machnik","full_name":"Machnik, Nick N","orcid":"0000-0001-6617-9742","first_name":"Nick N","id":"3591A0AA-F248-11E8-B48F-1D18A9856A87"},{"id":"46046B7A-F248-11E8-B48F-1D18A9856A87","first_name":"Roshan K","full_name":"Satapathy, Roshan K","last_name":"Satapathy"},{"orcid":"0000-0001-6197-363X","id":"3981F020-F248-11E8-B48F-1D18A9856A87","first_name":"Isabella","full_name":"Tomanek, Isabella","last_name":"Tomanek"}],"date_updated":"2024-09-16T06:03:22Z","ddc":["510","530","570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2020","oa_version":"None","title":"Core Project Proceedings","date_created":"2020-07-22T14:48:14Z","date_published":"2020-01-28T00:00:00Z","publication_status":"published","type":"report","_id":"8151","language":[{"iso":"eng"}],"abstract":[{"text":"The main idea behind the Core Project is to teach first year students at IST scientific communication skills and let them practice by presenting their research within an interdisciplinary environment. Over the course of the first semester, students participated in seminars, where they shared their results with the colleagues from other fields and took part in discussions on relevant subjects. The main focus during this sessions was on delivering the information in a simplified and comprehensible way, going into the very basics of a subject if necessary. At the end, the students were asked to present their research in the written form to exercise their writing skills. The reports were gathered in this document. All of them were reviewed by the  teaching assistants and write-ups illustrating unique stylistic features and, in general, an outstanding level of writing skills, were honorably mentioned in the section \"Selected Reports\".","lang":"eng"}],"file":[{"access_level":"local","content_type":"application/pdf","file_size":169620437,"date_created":"2020-07-22T14:45:07Z","file_name":"Core_Project_Proceedings_mod.pdf","relation":"main_file","date_updated":"2020-07-22T14:45:07Z","creator":"dernst","file_id":"8152"}],"day":"28","citation":{"ama":"Maslov M, Kondrashov F, Artner C, et al. <i>Core Project Proceedings</i>. IST Austria; 2020.","ista":"Maslov M, Kondrashov F, Artner C, Hennessey-Wesen M, Kavcic B, Machnik NN, Satapathy RK, Tomanek I. 2020. Core Project Proceedings, IST Austria, 425p.","chicago":"Maslov, Mikhail, Fyodor Kondrashov, Christina Artner, Mike Hennessey-Wesen, Bor Kavcic, Nick N Machnik, Roshan K Satapathy, and Isabella Tomanek. <i>Core Project Proceedings</i>. IST Austria, 2020.","mla":"Maslov, Mikhail, et al. <i>Core Project Proceedings</i>. IST Austria, 2020.","short":"M. Maslov, F. Kondrashov, C. Artner, M. Hennessey-Wesen, B. Kavcic, N.N. Machnik, R.K. Satapathy, I. Tomanek, Core Project Proceedings, IST Austria, 2020.","ieee":"M. Maslov <i>et al.</i>, <i>Core Project Proceedings</i>. IST Austria, 2020.","apa":"Maslov, M., Kondrashov, F., Artner, C., Hennessey-Wesen, M., Kavcic, B., Machnik, N. N., … Tomanek, I. (2020). <i>Core Project Proceedings</i>. IST Austria."},"has_accepted_license":"1"}]