---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18920'
abstract:
- lang: eng
  text: The globally distributed marine alga Emiliania huxleyi has cooling effect
    on the Earth’s climate. The population density of E. huxleyi is restricted by
    Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite
    the impact of E. huxleyi viruses on the climate, there is limited information
    about their structure and replication. Here, we show that the dsDNA genome inside
    the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane.
    EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse
    the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble
    in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments.
    Genome packaging initiates synchronously with the capsid assembly and completes
    through an aperture in the forming capsid. The genome-filled capsids acquire an
    outer membrane by budding into intracellular vesicles. EhV-201 infection induces
    a loss of surface protective layers from E. huxleyi cells, which enables the continuous
    release of virions by exocytosis.
acknowledgement: We acknowledge (i) the Cryo-Electron Microscopy and Tomography Core
  Facility and Proteomics Core Facility of the Central European Institute of Technology
  (CEITEC), Masaryk University, supported by the Ministry of Education, Youth, and
  Sports of the Czech Republic (grant LM2018127); (ii) the Cellular Imaging Core Facility
  supported by the Czech-BioImaging large RI project (LM2018129 funded by MEYS CR);
  and (iii) Plant Sciences Core Facility for support with obtaining scientific data
  presented here. We acknowledge support from the project National Institute of Virology
  and Bacteriology (Program EXCELES, ID project no. LX22NPO5103), funded by the European
  Union - Next Generation EU. This work received funding from the Czech Science Foundation
  grant GX 19-259882X to P.P., from European Regional Development Fund-Project “MSCAfellow2@MUNI”
  (no. CZ.02.2.69/0.0/0.0/18_070/0009846) to C.R.B., and from Brno PhD talent scholarship
  funded by Brno city municipality to M.H.
article_number: 'eadk1954 '
article_processing_charge: Yes
article_type: original
author:
- first_name: Miroslav
  full_name: Homola, Miroslav
  last_name: Homola
- first_name: Renate Carina
  full_name: Büttner, Renate Carina
  id: 3b7984c9-17ff-11ed-b6fe-f943c4a5b626
  last_name: Büttner
- first_name: Tibor
  full_name: Füzik, Tibor
  last_name: Füzik
- first_name: Pavel
  full_name: Křepelka, Pavel
  last_name: Křepelka
- first_name: Radka
  full_name: Holbová, Radka
  last_name: Holbová
- first_name: Jiří
  full_name: Nováček, Jiří
  last_name: Nováček
- first_name: Marten L.
  full_name: Chaillet, Marten L.
  last_name: Chaillet
- first_name: Jakub
  full_name: Žák, Jakub
  last_name: Žák
- first_name: Danyil
  full_name: Grybchuk, Danyil
  last_name: Grybchuk
- first_name: Friedrich
  full_name: Förster, Friedrich
  last_name: Förster
- first_name: William H.
  full_name: Wilson, William H.
  last_name: Wilson
- first_name: Declan C.
  full_name: Schroeder, Declan C.
  last_name: Schroeder
- first_name: Pavel
  full_name: Plevka, Pavel
  last_name: Plevka
citation:
  ama: Homola M, Büttner RC, Füzik T, et al. Structure and replication cycle of a
    virus infecting climate-modulating alga Emiliania huxleyi. <i>Science Advances</i>.
    2024;10(15). doi:<a href="https://doi.org/10.1126/sciadv.adk1954">10.1126/sciadv.adk1954</a>
  apa: Homola, M., Büttner, R. C., Füzik, T., Křepelka, P., Holbová, R., Nováček,
    J., … Plevka, P. (2024). Structure and replication cycle of a virus infecting
    climate-modulating alga Emiliania huxleyi. <i>Science Advances</i>. American Association
    for the Advancement of Science. <a href="https://doi.org/10.1126/sciadv.adk1954">https://doi.org/10.1126/sciadv.adk1954</a>
  chicago: Homola, Miroslav, Renate Carina Büttner, Tibor Füzik, Pavel Křepelka, Radka
    Holbová, Jiří Nováček, Marten L. Chaillet, et al. “Structure and Replication Cycle
    of a Virus Infecting Climate-Modulating Alga Emiliania Huxleyi.” <i>Science Advances</i>.
    American Association for the Advancement of Science, 2024. <a href="https://doi.org/10.1126/sciadv.adk1954">https://doi.org/10.1126/sciadv.adk1954</a>.
  ieee: M. Homola <i>et al.</i>, “Structure and replication cycle of a virus infecting
    climate-modulating alga Emiliania huxleyi,” <i>Science Advances</i>, vol. 10,
    no. 15. American Association for the Advancement of Science, 2024.
  ista: Homola M, Büttner RC, Füzik T, Křepelka P, Holbová R, Nováček J, Chaillet
    ML, Žák J, Grybchuk D, Förster F, Wilson WH, Schroeder DC, Plevka P. 2024. Structure
    and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi.
    Science Advances. 10(15), eadk1954.
  mla: Homola, Miroslav, et al. “Structure and Replication Cycle of a Virus Infecting
    Climate-Modulating Alga Emiliania Huxleyi.” <i>Science Advances</i>, vol. 10,
    no. 15, eadk1954, American Association for the Advancement of Science, 2024, doi:<a
    href="https://doi.org/10.1126/sciadv.adk1954">10.1126/sciadv.adk1954</a>.
  short: M. Homola, R.C. Büttner, T. Füzik, P. Křepelka, R. Holbová, J. Nováček, M.L.
    Chaillet, J. Žák, D. Grybchuk, F. Förster, W.H. Wilson, D.C. Schroeder, P. Plevka,
    Science Advances 10 (2024).
date_created: 2025-01-27T14:32:34Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2025-05-14T09:29:04Z
day: '01'
ddc:
- '570'
department:
- _id: EM-Fac
doi: 10.1126/sciadv.adk1954
external_id:
  pmid:
  - '38598627'
file:
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has_accepted_license: '1'
intvolume: '        10'
issue: '15'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
  eissn:
  - 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: ' https://github.com/fuzikt/tomostarpy.'
scopus_import: '1'
status: public
title: Structure and replication cycle of a virus infecting climate-modulating alga
  Emiliania huxleyi
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2024'
...