[{"publication_identifier":{"issn":["0960-9822"]},"oa":1,"acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","file":[{"date_updated":"2026-03-24T08:34:37Z","content_type":"application/pdf","checksum":"fe6c41fdab58a55df5f2a5860c02acdc","file_size":12986894,"file_name":"2026_CurrentBiology_Li.pdf","creator":"dernst","date_created":"2026-03-24T08:34:37Z","file_id":"21496","access_level":"open_access","relation":"main_file","success":1}],"pmid":1,"intvolume":" 36","publication":"Current Biology","file_date_updated":"2026-03-24T08:34:37Z","author":[{"full_name":"Li, Mingyue","last_name":"Li","id":"01f96916-0235-11eb-9379-a323192643b7","first_name":"Mingyue"},{"first_name":"Nikola","last_name":"Rydza","full_name":"Rydza, Nikola"},{"full_name":"Mazur, Ewa","last_name":"Mazur","first_name":"Ewa"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely","last_name":"Molnar","full_name":"Molnar, Gergely"},{"first_name":"Tomasz","full_name":"Nodzyński, Tomasz","last_name":"Nodzyński"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"}],"acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"abstract":[{"lang":"eng","text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development."}],"quality_controlled":"1","volume":36,"language":[{"iso":"eng"}],"corr_author":"1","department":[{"_id":"JiFr"}],"external_id":{"pmid":["41831441"]},"date_created":"2026-03-23T15:11:16Z","publication_status":"published","day":"23","publisher":"Elsevier","type":"journal_article","issue":"6","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","page":"1468-1480.e6","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2026","oa_version":"Published Version","date_updated":"2026-03-24T08:36:40Z","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Identification of a novel regulator in auxin canalization","_id":"bd906599-d553-11ed-ba76-abf8547645d7","grant_number":"E271"}],"date_published":"2026-03-23T00:00:00Z","has_accepted_license":"1","citation":{"apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023","ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023.","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026."},"PlanS_conform":"1","article_processing_charge":"Yes (via OA deal)","title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","month":"03","_id":"21490","OA_place":"publisher","doi":"10.1016/j.cub.2026.02.023","OA_type":"hybrid","article_type":"original","ddc":["580"]},{"OA_type":"hybrid","article_type":"original","doi":"10.1111/nph.71072","OA_place":"publisher","month":"03","title":"Imaging and genetic toolbox to study Arabidopsis embryogenesis","_id":"21483","PlanS_conform":"1","citation":{"apa":"Babic, D., Zupunski, M., & Friml, J. (2026). Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. Wiley. https://doi.org/10.1111/nph.71072","ama":"Babic D, Zupunski M, Friml J. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. 2026. doi:10.1111/nph.71072","ista":"Babic D, Zupunski M, Friml J. 2026. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist., nph. 71072.","chicago":"Babic, David, Milan Zupunski, and Jiří Friml. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist. Wiley, 2026. https://doi.org/10.1111/nph.71072.","mla":"Babic, David, et al. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” New Phytologist, nph. 71072, Wiley, 2026, doi:10.1111/nph.71072.","short":"D. Babic, M. Zupunski, J. Friml, New Phytologist (2026).","ieee":"D. Babic, M. Zupunski, and J. Friml, “Imaging and genetic toolbox to study Arabidopsis embryogenesis,” New Phytologist. Wiley, 2026."},"article_processing_charge":"Yes (via OA deal)","date_updated":"2026-03-30T05:58:35Z","year":"2026","oa_version":"Published Version","has_accepted_license":"1","date_published":"2026-03-11T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","day":"11","type":"journal_article","publisher":"Wiley","publication_status":"epub_ahead","corr_author":"1","language":[{"iso":"eng"}],"date_created":"2026-03-23T14:59:06Z","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"external_id":{"pmid":["41808651"]},"quality_controlled":"1","article_number":"nph.71072","abstract":[{"text":"Embryogenesis in the model plant Arabidopsis thaliana provides a framework for understanding how cell polarity and patterning coordinate with hormonal signalling to establish the plant body plan. Following fertilisation, the zygote divides asymmetrically to generate apical and basal lineages, establishing the apical–basal axis that defines future shoot and root poles. Genetic and molecular analyses of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless revealed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical–basal axis establishment and organ initiation. The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors. Advances in microscopy have transformed the study of Arabidopsis embryogenesis: fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation, and cytoskeletal dynamics. Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of embryo development, while laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity. Together, these methodological breakthroughs position Arabidopsis embryos as a prime model for dissecting the chemical and biophysical cues that shape plant development.","lang":"eng"}],"publication":"New Phytologist","author":[{"id":"db566d23-f6e0-11ea-865d-e6f270e968e7","first_name":"David","full_name":"Babic, David","last_name":"Babic"},{"first_name":"Milan","id":"f6a21fce-573e-11f0-a150-a8d96aee2539","last_name":"Zupunski","full_name":"Zupunski, Milan"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"oa":1,"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"pmid":1,"acknowledgement":"The authors would like to acknowledge the many colleagues whose valuable contributions to the field could not be included in this review due to space limitations and reference constraints. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ.","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.71072"}]},{"year":"2025","oa_version":"Published Version","date_updated":"2025-05-19T14:02:01Z","has_accepted_license":"1","date_published":"2025-01-13T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"status":"public","day":"13","type":"journal_article","publisher":"Elsevier","issue":"1","ddc":["580"],"doi":"10.1016/j.xplc.2024.101181","OA_place":"publisher","article_type":"original","OA_type":"gold","title":"Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1","month":"01","_id":"18619","citation":{"ista":"Wei H, Zhu H, Ying W, Janssens H, Kvasnica M, Winne J, Gao Y, Friml J, Ma Q, Tan S, Liu X, Russinova E, Sun L. 2025. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 6(1), 101181.","chicago":"Wei, H, H Zhu, W Ying, H Janssens, M Kvasnica, JM Winne, Y Gao, et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications. Elsevier, 2025. https://doi.org/10.1016/j.xplc.2024.101181.","apa":"Wei, H., Zhu, H., Ying, W., Janssens, H., Kvasnica, M., Winne, J., … Sun, L. (2025). Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2024.101181","ama":"Wei H, Zhu H, Ying W, et al. Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. Plant Communications. 2025;6(1). doi:10.1016/j.xplc.2024.101181","ieee":"H. Wei et al., “Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1,” Plant Communications, vol. 6, no. 1. Elsevier, 2025.","short":"H. Wei, H. Zhu, W. Ying, H. Janssens, M. Kvasnica, J. Winne, Y. Gao, J. Friml, Q. Ma, S. Tan, X. Liu, E. Russinova, L. Sun, Plant Communications 6 (2025).","mla":"Wei, H., et al. “Structural Insights into Brassinosteroid Export Mediated by the Arabidopsis ABC Transporter ABCB1.” Plant Communications, vol. 6, no. 1, 101181, Elsevier, 2025, doi:10.1016/j.xplc.2024.101181."},"article_processing_charge":"Yes","intvolume":" 6","isi":1,"scopus_import":"1","file_date_updated":"2025-04-16T09:02:05Z","publication":"Plant Communications","author":[{"last_name":"Wei","full_name":"Wei, H","first_name":"H"},{"last_name":"Zhu","full_name":"Zhu, H","first_name":"H"},{"last_name":"Ying","full_name":"Ying, W","first_name":"W"},{"first_name":"H","full_name":"Janssens, H","last_name":"Janssens"},{"first_name":"M","full_name":"Kvasnica, M","last_name":"Kvasnica"},{"first_name":"JM","last_name":"Winne","full_name":"Winne, JM"},{"first_name":"Y","full_name":"Gao, Y","last_name":"Gao"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"first_name":"Q","full_name":"Ma, Q","last_name":"Ma"},{"last_name":"Tan","full_name":"Tan, S","first_name":"S"},{"last_name":"Liu","full_name":"Liu, X","first_name":"X"},{"first_name":"E","last_name":"Russinova","full_name":"Russinova, E"},{"first_name":"L","last_name":"Sun","full_name":"Sun, L"}],"oa":1,"publication_identifier":{"issn":["2590-3462"]},"DOAJ_listed":"1","file":[{"date_updated":"2025-04-16T09:02:05Z","content_type":"application/pdf","checksum":"7b0e4511e43cc0da06730c3edb7c1167","file_size":4443183,"file_name":"2025_PlantComm_Wei.pdf","creator":"dernst","date_created":"2025-04-16T09:02:05Z","file_id":"19575","access_level":"open_access","relation":"main_file","success":1}],"acknowledgement":"We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support. We thank Yaowei Wang, Yongming Luo, and Nemanja Vukašinović (VIB-UGhent, Belgium) for useful discussions and technical support. L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation. No conflict of interest is declared.","pmid":1,"publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"external_id":{"pmid":["39497419"],"isi":["001416757300001"]},"date_created":"2024-12-04T11:21:16Z","quality_controlled":"1","volume":6,"abstract":[{"text":"Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signalling initiation, which requires binding of BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter, ABCB19, functions as a BR exporter, and together with its close homologue, ABCB1, positively regulate BR signalling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 was stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states were determined. In the BL-bound structure, BL was bound to the hydrophobic cavity formed by the transmembrane domain, and triggered local conformational changes. Together, our data provide additional insights into the ABC transporter-mediated BR export.","lang":"eng"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","article_number":"101181"},{"isi":1,"intvolume":" 44","publication":"Cell Reports","file_date_updated":"2025-07-22T08:52:17Z","scopus_import":"1","author":[{"id":"56aad729-cca2-11ed-a45a-9b4138991a48","first_name":"Bin","last_name":"Guan","full_name":"Guan, Bin"},{"first_name":"Ke Xuan","last_name":"Xie","full_name":"Xie, Ke Xuan"},{"first_name":"Xin Qiao","full_name":"Du, Xin Qiao","last_name":"Du"},{"full_name":"Bai, Yu Xuan","last_name":"Bai","first_name":"Yu Xuan"},{"first_name":"Peng Chao","last_name":"Hao","full_name":"Hao, Peng Chao"},{"full_name":"Lin, Wen Hui","last_name":"Lin","first_name":"Wen Hui"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"first_name":"Hong Wei","last_name":"Xue","full_name":"Xue, Hong Wei"}],"publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"oa":1,"acknowledgement":"The study was supported by National Natural Science Foundation of China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were run on the Siyuan-1 cluster supported by the Center for High-Performance Computing at Shanghai Jiao Tong University.","file":[{"success":1,"access_level":"open_access","relation":"main_file","file_size":37708120,"creator":"dernst","file_name":"2025_CellReports_Guan.pdf","file_id":"20067","date_created":"2025-07-22T08:52:17Z","date_updated":"2025-07-22T08:52:17Z","content_type":"application/pdf","checksum":"ee03deee47a084b0295251dc49470ad4"}],"pmid":1,"publication_status":"published","language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"external_id":{"pmid":["40668679"],"isi":["001533244800001"]},"date_created":"2025-07-20T22:02:01Z","quality_controlled":"1","volume":44,"abstract":[{"text":"Vacuolar acidification is crucial for the homeostasis of intracellular pH and the recycling of proteins and nutrients in cells, thereby playing important roles in various physiological processes related to vacuolar function. The key factors regulating vacuolar acidification and underlying mechanisms remain unclear. Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency. The pldζ2 mutant massively accumulates autophagic structures while exhibiting premature leaf senescence under nutrient starvation. Impaired autophagic flux, lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2 directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote vacuolar acidification and autophagy under phosphorus starvation. These findings reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide clues in elucidating the regulatory mechanism of vacuolar acidification.","lang":"eng"}],"license":"https://creativecommons.org/licenses/by-nc/4.0/","article_number":"116024","year":"2025","oa_version":"Published Version","date_updated":"2025-09-30T14:05:28Z","date_published":"2025-07-22T00:00:00Z","has_accepted_license":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"status":"public","day":"22","type":"journal_article","publisher":"Elsevier","issue":"7","ddc":["580"],"OA_place":"publisher","doi":"10.1016/j.celrep.2025.116024","article_type":"original","OA_type":"hybrid","title":"Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD","month":"07","_id":"20029","citation":{"apa":"Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue, H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2025.116024","ama":"Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 2025;44(7). doi:10.1016/j.celrep.2025.116024","ista":"Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 44(7), 116024.","chicago":"Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” Cell Reports. Elsevier, 2025. https://doi.org/10.1016/j.celrep.2025.116024.","short":"B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W. Xue, Cell Reports 44 (2025).","mla":"Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” Cell Reports, vol. 44, no. 7, 116024, Elsevier, 2025, doi:10.1016/j.celrep.2025.116024.","ieee":"B. Guan et al., “Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD,” Cell Reports, vol. 44, no. 7. Elsevier, 2025."},"article_processing_charge":"Yes (in subscription journal)"},{"file_date_updated":"2025-11-24T13:48:09Z","author":[{"first_name":"F","last_name":"Sheng","full_name":"Sheng, F"},{"full_name":"Gao, Y","last_name":"Gao","first_name":"Y"},{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"last_name":"Li","full_name":"Li, Y","first_name":"Y"},{"first_name":"JA","last_name":"Zhang","full_name":"Zhang, JA"},{"full_name":"Zhang, Z","last_name":"Zhang","first_name":"Z"},{"full_name":"Qin, X","last_name":"Qin","first_name":"X"},{"first_name":"S","last_name":"Zhang","full_name":"Zhang, S"},{"first_name":"W","full_name":"Song, W","last_name":"Song"},{"full_name":"Li, J","last_name":"Li","first_name":"J"},{"full_name":"Guo, Y","last_name":"Guo","first_name":"Y"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Gong, Z","last_name":"Gong","first_name":"Z"},{"first_name":"Q","last_name":"Zhang","full_name":"Zhang, Q"},{"first_name":"J","last_name":"Zhang","full_name":"Zhang, J"}],"publication":"Proceedings of the National Academy of Sciences","scopus_import":"1","isi":1,"intvolume":" 122","acknowledgement":"This research was funded by Biological Breeding-National Science and Technology Major Project (2023ZD0407201), China Postdoctoral Science Foundation (2024M763575), China Agricultural University Fund (2025RC042), Chinese Universities Scientific Fund (2024RC031), and Austrian Science Fund (FWF; I 6123-B).","file":[{"relation":"main_file","access_level":"open_access","success":1,"date_updated":"2025-11-24T13:48:09Z","checksum":"38b723a909bf321d7ee537c9d064aa25","content_type":"application/pdf","file_size":2667764,"date_created":"2025-11-24T13:48:09Z","file_id":"20681","creator":"dernst","file_name":"2025_PNAS_Sheng.pdf"}],"pmid":1,"oa":1,"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"external_id":{"pmid":["40986351"],"isi":["001589177800001"]},"department":[{"_id":"JiFr"}],"date_created":"2025-11-12T10:03:20Z","language":[{"iso":"eng"}],"publication_status":"published","abstract":[{"text":"Plants have evolved sophisticated mechanisms to adapt to environmental changes, with root gravitropism playing a pivotal role in nutrient and water acquisition. Our study reveals that SnRK2 kinases (SnRK2.2 and SnRK2.3) are critical regulators of root gravitropism through their direct phosphorylation of the auxin transporter PIN2 at S259. We demonstrate that SnRK2s-mediated phosphorylation modulates both the polar localization and transport activity of PIN2. Importantly, SnRK2s function antagonistically to the AGCVIII kinase PID, which phosphorylates PIN2 at a distinct site (S258), establishing a regulatory balance essential for adaptive root growth. Structural modeling and phosphorylation assays further suggest that SnRK2s-mediated phosphorylation at S259 sterically hinders access of PID to S258, providing a mechanistic basis for their antagonistic relationship. These findings uncover a novel regulatory mechanism, by which plants fine-tune root developmental programs to adapt to environmental stimuli, highlighting the evolutionary significance of multilayered kinase-mediated regulation in plant adaptation.","lang":"eng"}],"volume":122,"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"e2512274122","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"}],"date_published":"2025-09-23T00:00:00Z","has_accepted_license":"1","year":"2025","oa_version":"Published Version","date_updated":"2026-02-16T12:32:51Z","publisher":"National Academy of Sciences","type":"journal_article","day":"23","issue":"39","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"doi":"10.1073/pnas.2512274122","OA_place":"publisher","article_type":"original","OA_type":"hybrid","ddc":["580"],"article_processing_charge":"Yes (in subscription journal)","citation":{"mla":"Sheng, F., et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” Proceedings of the National Academy of Sciences, vol. 122, no. 39, National Academy of Sciences, 2025, p. e2512274122, doi:10.1073/pnas.2512274122.","short":"F. Sheng, Y. Gao, Y. Wang, Y. Li, J. Zhang, Z. Zhang, X. Qin, S. Zhang, W. Song, J. Li, Y. Guo, J. Friml, Z. Gong, Q. Zhang, J. Zhang, Proceedings of the National Academy of Sciences 122 (2025) e2512274122.","ieee":"F. Sheng et al., “Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism,” Proceedings of the National Academy of Sciences, vol. 122, no. 39. National Academy of Sciences, p. e2512274122, 2025.","apa":"Sheng, F., Gao, Y., Wang, Y., Li, Y., Zhang, J., Zhang, Z., … Zhang, J. (2025). Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2512274122","ama":"Sheng F, Gao Y, Wang Y, et al. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 2025;122(39):e2512274122. doi:10.1073/pnas.2512274122","ista":"Sheng F, Gao Y, Wang Y, Li Y, Zhang J, Zhang Z, Qin X, Zhang S, Song W, Li J, Guo Y, Friml J, Gong Z, Zhang Q, Zhang J. 2025. Antagonistic SnRK2 and PID kinases’ action on auxin transport-mediated root gravitropism. Proceedings of the National Academy of Sciences. 122(39), e2512274122.","chicago":"Sheng, F, Y Gao, Y Wang, Y Li, JA Zhang, Z Zhang, X Qin, et al. “Antagonistic SnRK2 and PID Kinases’ Action on Auxin Transport-Mediated Root Gravitropism.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2512274122."},"PlanS_conform":"1","_id":"20635","title":"Antagonistic SnRK2 and PID kinases' action on auxin transport-mediated root gravitropism","month":"09"},{"author":[{"last_name":"Huang","full_name":"Huang, R","first_name":"R"},{"first_name":"J","last_name":"Wang","full_name":"Wang, J"},{"first_name":"M","last_name":"Chang","full_name":"Chang, M"},{"first_name":"W","last_name":"Tang","full_name":"Tang, W"},{"last_name":"Yu","full_name":"Yu, Y","first_name":"Y"},{"first_name":"Y","full_name":"Zhang, Y","last_name":"Zhang"},{"last_name":"Peng","full_name":"Peng, Y","first_name":"Y"},{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"first_name":"Y","last_name":"Guo","full_name":"Guo, Y"},{"last_name":"Lu","full_name":"Lu, T","first_name":"T"},{"full_name":"Cao, Y","last_name":"Cao","first_name":"Y"},{"full_name":"Zhou, Y","last_name":"Zhou","first_name":"Y"},{"first_name":"Q","last_name":"Zhang","full_name":"Zhang, Q"},{"first_name":"Y","last_name":"Huang","full_name":"Huang, Y"},{"last_name":"Wu","full_name":"Wu, A","first_name":"A"},{"last_name":"Ren","full_name":"Ren, L","first_name":"L"},{"orcid":"0000-0003-1286-7368","last_name":"Gallei","full_name":"Gallei, Michelle C","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"J","full_name":"Dong, J","last_name":"Dong"},{"full_name":"Chen, H","last_name":"Chen","first_name":"H"},{"full_name":"He, J","last_name":"He","first_name":"J"},{"last_name":"Wen","full_name":"Wen, M","first_name":"M"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Sun, L","last_name":"Sun","first_name":"L"},{"first_name":"Y","full_name":"Xiong, Y","last_name":"Xiong"},{"first_name":"Z","last_name":"Yang","full_name":"Yang, Z"},{"first_name":"T","full_name":"Xu, T","last_name":"Xu"}],"scopus_import":"1","publication":"Developmental Cell","acknowledgement":"We thank Lukáš Fiedler for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","pmid":1,"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"publication_status":"epub_ahead","external_id":{"pmid":["41043435"]},"department":[{"_id":"JiFr"}],"date_created":"2025-11-12T10:03:39Z","language":[{"iso":"eng"}],"quality_controlled":"1","abstract":[{"lang":"eng","text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis."}],"date_published":"2025-10-02T00:00:00Z","oa_version":"None","year":"2025","date_updated":"2025-11-24T13:43:08Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"S1534-5807(25)00569-6","status":"public","publisher":"Elsevier","day":"02","type":"journal_article","doi":"10.1016/j.devcel.2025.09.009","article_type":"original","OA_type":"closed access","_id":"20636","title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","month":"10","article_processing_charge":"No","citation":{"ieee":"R. Huang et al., “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” Developmental Cell. Elsevier, pp. S1534-5807(25)00569–6, 2025.","mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell, Elsevier, 2025, pp. S1534-5807(25)00569-6, doi:10.1016/j.devcel.2025.09.009.","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell (2025) S1534-5807(25)00569–6.","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2025. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell., S1534-5807(25)00569–6.","chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.09.009.","apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2025). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.09.009","ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 2025:S1534-5807(25)00569-6. doi:10.1016/j.devcel.2025.09.009"}},{"type":"journal_article","publisher":"Elsevier","day":"30","issue":"22","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"6138-6150.e17","has_accepted_license":"1","project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"},{"_id":"26060676-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 985-2016","name":"Cell surface receptor complexes for auxin signaling in plants"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"}],"date_published":"2025-10-30T00:00:00Z","oa_version":"Published Version","year":"2025","date_updated":"2025-12-01T15:27:22Z","article_processing_charge":"Yes (via OA deal)","citation":{"mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell, vol. 188, no. 22, Elsevier, 2025, p. 6138–6150.e17, doi:10.1016/j.cell.2025.08.026.","short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, I. Kulich, Y. Jaillais, J. Friml, Cell 188 (2025) 6138–6150.e17.","ieee":"L. Rodriguez Solovey et al., “ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism,” Cell, vol. 188, no. 22. Elsevier, p. 6138–6150.e17, 2025.","ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 2025;188(22):6138-6150.e17. doi:10.1016/j.cell.2025.08.026","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (2025). ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. Elsevier. https://doi.org/10.1016/j.cell.2025.08.026","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell. Elsevier, 2025. https://doi.org/10.1016/j.cell.2025.08.026.","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Kulich I, Jaillais Y, Friml J. 2025. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 188(22), 6138–6150.e17."},"PlanS_conform":"1","related_material":{"record":[{"relation":"earlier_version","status":"public","id":"19399"}]},"_id":"20656","title":"ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism","month":"10","doi":"10.1016/j.cell.2025.08.026","OA_place":"publisher","article_type":"original","OA_type":"hybrid","ddc":["580"],"file":[{"file_size":17825465,"creator":"dernst","file_name":"2025_Cell_Rodriguez.pdf","file_id":"20679","date_created":"2025-11-24T10:55:18Z","date_updated":"2025-11-24T10:55:18Z","checksum":"8ac396a0806ad7f2e4e7a0c1eed712ce","content_type":"application/pdf","success":1,"access_level":"open_access","relation":"main_file"}],"acknowledgement":"We gratefully acknowledge Tongda Xu for experimental, material, and conceptual support. We thank William Gray for providing material, Nataliia Gnyliukh and Ema Cervenova for help with manuscript preparation, and Julia Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at the Institute of Science and Technology Austria (ISTA) for their excellent service and assistance. The research leading to these results has received funding from the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; I 6123-B) to J.F., and Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by the FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985-2016). S.T. was supported by the National Natural Science Foundation of China (32321001, 32570366). The work of J.H. was supported by the project JG_2024_003 implemented within the Palacký University Young Researcher Grant.","pmid":1,"oa":1,"publication_identifier":{"issn":["0092-8674"]},"publication":"Cell","author":[{"last_name":"Rodriguez Solovey","full_name":"Rodriguez Solovey, Lesia","first_name":"Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7244-7237"},{"full_name":"Fiedler, Lukas","last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986","first_name":"Lukas"},{"first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","last_name":"Zou"},{"last_name":"Giannini","full_name":"Giannini, Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","first_name":"Caterina"},{"full_name":"Monzer, Aline","last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","first_name":"Aline"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev"},{"full_name":"Randuch, Marek","last_name":"Randuch","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"full_name":"Yu, Yongfan","last_name":"Yu","first_name":"Yongfan"},{"orcid":"0000-0003-4783-1752","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","first_name":"Zuzana","full_name":"Gelová, Zuzana","last_name":"Gelová"},{"orcid":"0000-0001-7241-2328","first_name":"Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","full_name":"Verstraeten, Inge"},{"last_name":"Hajny","full_name":"Hajny, Jakub","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195"},{"first_name":"Meng","full_name":"Chen, Meng","last_name":"Chen"},{"orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","last_name":"Tan"},{"full_name":"Hörmayer, Lukas","last_name":"Hörmayer","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","first_name":"Lukas","orcid":"0000-0001-8295-2926"},{"orcid":"0000-0002-5607-272X","last_name":"Li","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","first_name":"Lanxin"},{"first_name":"Maria Mar","full_name":"Marques-Bueno, Maria Mar","last_name":"Marques-Bueno"},{"full_name":"Quddoos, Zainab","last_name":"Quddoos","id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466","first_name":"Zainab"},{"last_name":"Molnar","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely"},{"full_name":"Kulich, Ivan","last_name":"Kulich","first_name":"Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"first_name":"Yvon","full_name":"Jaillais, Yvon","last_name":"Jaillais"},{"last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"file_date_updated":"2025-11-24T10:55:18Z","ec_funded":1,"isi":1,"intvolume":" 188","abstract":[{"text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a prerequisite for (1) self-organizing processes, including vascular tissue formation, and (2) directional growth responses such as gravitropism. Here, we identify a mechanism by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1) receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably for other self-organizing developmental phenomena.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"volume":188,"quality_controlled":"1","external_id":{"pmid":["41043433"],"isi":["001616077900005"]},"department":[{"_id":"JiFr"},{"_id":"XiFe"}],"date_created":"2025-11-19T09:44:31Z","language":[{"iso":"eng"}],"corr_author":"1","publication_status":"published"},{"citation":{"apa":"Roychoudhry, S., Sageman-Furnas, K., Taylor, H. J., Showpnil, I., Wolverton, C., Friml, J., … Kepinski, S. (2025). Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2506400122","ama":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, et al. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 2025;122(46):e2506400122. doi:10.1073/pnas.2506400122","ista":"Roychoudhry S, Sageman-Furnas K, Taylor HJ, Showpnil I, Wolverton C, Friml J, Bianco MD, Kepinski S. 2025. Angle dependence as a unifying feature of root graviresponse modules. Proceedings of the National Academy of Sciences. 122(46), e2506400122.","chicago":"Roychoudhry, Suruchi, Katelyn Sageman-Furnas, Harry J. Taylor, Iftekhar Showpnil, Chris Wolverton, Jiří Friml, Marta Del Bianco, and Stefan Kepinski. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” Proceedings of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2506400122.","short":"S. Roychoudhry, K. Sageman-Furnas, H.J. Taylor, I. Showpnil, C. Wolverton, J. Friml, M.D. Bianco, S. Kepinski, Proceedings of the National Academy of Sciences 122 (2025) e2506400122.","mla":"Roychoudhry, Suruchi, et al. “Angle Dependence as a Unifying Feature of Root Graviresponse Modules.” Proceedings of the National Academy of Sciences, vol. 122, no. 46, National Academy of Sciences, 2025, p. e2506400122, doi:10.1073/pnas.2506400122.","ieee":"S. Roychoudhry et al., “Angle dependence as a unifying feature of root graviresponse modules,” Proceedings of the National Academy of Sciences, vol. 122, no. 46. National Academy of Sciences, p. e2506400122, 2025."},"PlanS_conform":"1","article_processing_charge":"Yes (in subscription journal)","title":"Angle dependence as a unifying feature of root graviresponse modules","month":"11","_id":"20663","OA_place":"publisher","doi":"10.1073/pnas.2506400122","article_type":"original","OA_type":"hybrid","ddc":["580"],"publisher":"National Academy of Sciences","type":"journal_article","day":"18","issue":"46","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","page":"e2506400122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","oa_version":"Published Version","date_updated":"2026-02-16T12:31:31Z","date_published":"2025-11-18T00:00:00Z","has_accepted_license":"1","abstract":[{"text":"Gravitropism, the patterning of postembryonic growth in relation to the gravity vector, allows plants to optimize the use of limited and nonhomogenous resources in their immediate environment. Since the current model of root gravitropism has not been able to integrate all aspects of the response (perception, response, and behavior), research on gravitropism has been dominated by different theories attempting to conceptualize each aspect individually. In this work, we sought to reevaluate all the main components of the root graviresponse through the lens of angle dependence. We show angle dependence in Cholodny–Went-based auxin asymmetry and growth response, which we tracked back to angle-dependent variation in PIN asymmetry and statolith sedimentation in the columella. Thanks to this approach, we were able to suggest distinct roles for PINs and columella cell tiers, and a potential function for auxin vertical flux through the columella. Our findings provide a unifying framework to further explore the mechanisms that regulate angle-dependent gravitropic response, with major implications of time-dependent features of root graviresponse.","lang":"eng"}],"quality_controlled":"1","volume":122,"language":[{"iso":"eng"}],"department":[{"_id":"JiFr"}],"external_id":{"pmid":["41218119"]},"date_created":"2025-11-23T23:01:38Z","publication_status":"published","oa":1,"publication_identifier":{"eissn":["1091-6490"]},"file":[{"date_updated":"2025-11-24T09:48:44Z","content_type":"application/pdf","checksum":"5e1c37dddc5db8fbd0128db4a54c4f6b","file_size":1394055,"file_id":"20676","date_created":"2025-11-24T09:48:44Z","creator":"dernst","file_name":"2025_PNAS_Roychoudhry.pdf","relation":"main_file","access_level":"open_access","success":1}],"acknowledgement":"This study was funded by the BBSRC (grant no. BB/N010124/1) and Leverhulme Foundation (grant no. RPG-2018-137) to M.D.B. and S.K., a Fully Funded International Research Scholarship awarded to K.S.-F., and by NASA (grant no. 80NSSC21K0585) to C.W.","pmid":1,"intvolume":" 122","publication":"Proceedings of the National Academy of Sciences","author":[{"full_name":"Roychoudhry, Suruchi","last_name":"Roychoudhry","first_name":"Suruchi"},{"last_name":"Sageman-Furnas","full_name":"Sageman-Furnas, Katelyn","first_name":"Katelyn"},{"first_name":"Harry J.","last_name":"Taylor","full_name":"Taylor, Harry J."},{"first_name":"Iftekhar","full_name":"Showpnil, Iftekhar","last_name":"Showpnil"},{"first_name":"Chris","full_name":"Wolverton, Chris","last_name":"Wolverton"},{"full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"first_name":"Marta Del","last_name":"Bianco","full_name":"Bianco, Marta Del"},{"first_name":"Stefan","full_name":"Kepinski, Stefan","last_name":"Kepinski"}],"file_date_updated":"2025-11-24T09:48:44Z","scopus_import":"1"},{"article_processing_charge":"Yes (via OA deal)","citation":{"ieee":"J. Friml, “Role of cAMP in TIR1/AFB auxin signaling: Open issues,” Trends in Plant Science. Elsevier, pp. S1360-1385(25)00300–0, 2025.","mla":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” Trends in Plant Science, Elsevier, 2025, pp. S1360-1385(25)00300-0, doi:10.1016/j.tplants.2025.10.018.","short":"J. Friml, Trends in Plant Science (2025) S1360-1385(25)00300–0.","chicago":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” Trends in Plant Science. Elsevier, 2025. https://doi.org/10.1016/j.tplants.2025.10.018.","ista":"Friml J. 2025. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science., S1360-1385(25)00300–0.","ama":"Friml J. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 2025:S1360-1385(25)00300-0. doi:10.1016/j.tplants.2025.10.018","apa":"Friml, J. (2025). Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. Elsevier. https://doi.org/10.1016/j.tplants.2025.10.018"},"PlanS_conform":"1","_id":"20725","title":"Role of cAMP in TIR1/AFB auxin signaling: Open issues","month":"11","OA_place":"publisher","doi":"10.1016/j.tplants.2025.10.018","OA_type":"hybrid","article_type":"review","ddc":["580"],"day":"16","publisher":"Elsevier","type":"journal_article","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"S1360-1385(25)00300-0","has_accepted_license":"1","date_published":"2025-11-16T00:00:00Z","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"year":"2025","oa_version":"Published Version","date_updated":"2025-12-09T08:04:58Z","abstract":[{"text":"The canonical mechanism by which the phytohormone auxin regulates transcription has been one of the cornerstones of plant signaling. The recent unexpected discovery of cyclic AMP (cAMP) as a second messenger in this pathway has revised its foundations while leaving many open questions and gaps in our understanding; these will be discussed in this forum article.","lang":"eng"}],"department":[{"_id":"JiFr"}],"external_id":{"pmid":["41249070"]},"date_created":"2025-12-02T16:29:22Z","language":[{"iso":"eng"}],"corr_author":"1","publication_status":"epub_ahead","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.tplants.2025.10.018"}],"acknowledgement":"I apologize to colleagues whose relevant work I was unable to cite due to space limitations. This work was funded by the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; 37051-B). I thank Drs Huihuang Chen, Yuanrong Pei, Jason Reed, Linlin Qi, and Dolf Weijers for inspiration and critical input.","pmid":1,"oa":1,"publication_identifier":{"eissn":["1878-4372"],"issn":["1360-1385"]},"publication":"Trends in Plant Science","author":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"scopus_import":"1"},{"citation":{"chicago":"Tang, Han, Adrijana Smoljan, Minxia Zou, Yuzhou Zhang, Kuan Ju Lu, and Jiří Friml. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment. Wiley, 2025. https://doi.org/10.1111/pce.70295.","ista":"Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. 2025. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment.","ama":"Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. 2025. doi:10.1111/pce.70295","apa":"Tang, H., Smoljan, A., Zou, M., Zhang, Y., Lu, K. J., & Friml, J. (2025). The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. Wiley. https://doi.org/10.1111/pce.70295","ieee":"H. Tang, A. Smoljan, M. Zou, Y. Zhang, K. J. Lu, and J. Friml, “The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha,” Plant Cell and Environment. Wiley, 2025.","short":"H. Tang, A. Smoljan, M. Zou, Y. Zhang, K.J. Lu, J. Friml, Plant Cell and Environment (2025).","mla":"Tang, Han, et al. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment, Wiley, 2025, doi:10.1111/pce.70295."},"article_processing_charge":"No","month":"12","title":"The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha","_id":"20818","article_type":"comment","OA_type":"closed access","doi":"10.1111/pce.70295","day":"03","publisher":"Wiley","type":"journal_article","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2025-12-15T13:56:26Z","oa_version":"None","year":"2025","date_published":"2025-12-03T00:00:00Z","project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"abstract":[{"text":"This study demonstrates that Marchantia non-canonical PINs are predominantly localized to the plasma membrane, with MpPINX and MpPINW exhibiting asymmetric distribution.\r\nA newly identified miniW domain within the MpPINW hydrophilic loop governs subcellular trafficking and asymmetric PM localization of non-canonical PINs in Marchantia.","lang":"eng"}],"quality_controlled":"1","language":[{"iso":"eng"}],"date_created":"2025-12-14T23:02:05Z","department":[{"_id":"JiFr"}],"external_id":{"pmid":["41340422"]},"publication_status":"epub_ahead","publication_identifier":{"issn":["0140-7791"],"eissn":["1365-3040"]},"pmid":1,"acknowledgement":"The authors sincerely thank Dr. Shutang Tan for experimental support and Dr. Barbara Kloeckener Gruissem for critical reading and constructive advice on the manuscript. This study was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.), by the Ministry of Science and Technology (grant 112-2636-B-005-001- to K.-J.L.), and by the Ministry of Education (grant MOE-109-YSFAG-0006-001-P1 to K.-J.L.).","ec_funded":1,"author":[{"orcid":"0000-0001-6152-6637","last_name":"Tang","full_name":"Tang, Han","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","first_name":"Han"},{"full_name":"Smoljan, Adrijana","last_name":"Smoljan","first_name":"Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b"},{"first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","last_name":"Zou"},{"last_name":"Zhang","full_name":"Zhang, Yuzhou","first_name":"Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956"},{"first_name":"Kuan Ju","full_name":"Lu, Kuan Ju","last_name":"Lu"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"publication":"Plant Cell and Environment","scopus_import":"1"},{"article_processing_charge":"Yes (via OA deal)","related_material":{"record":[{"id":"18689","relation":"earlier_version","status":"public"},{"id":"18837","status":"public","relation":"research_data"}]},"PlanS_conform":"1","citation":{"ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. The Plant Cell. 2025;37(4). doi:10.1093/plcell/koaf006","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. The Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koaf006","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell. Oxford University Press, 2025. https://doi.org/10.1093/plcell/koaf006.","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” The Plant Cell, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:10.1093/plcell/koaf006.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","ieee":"M. C. Gallei et al., “Super-resolution expansion microscopy in plant roots,” The Plant Cell, vol. 37, no. 4. Oxford University Press, 2025."},"_id":"19003","month":"04","title":"Super-resolution expansion microscopy in plant roots","article_type":"original","OA_type":"hybrid","OA_place":"publisher","doi":"10.1093/plcell/koaf006","ddc":["580"],"issue":"4","publisher":"Oxford University Press","type":"journal_article","day":"01","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","call_identifier":"H2020","name":"International IST Doctoral Program"},{"grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships"},{"name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","_id":"269B5B22-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 679-2018"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","grant_number":"26137","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","call_identifier":"FWF","name":"Molecular Drug Targets"}],"date_published":"2025-04-01T00:00:00Z","has_accepted_license":"1","date_updated":"2025-10-08T08:43:56Z","year":"2025","oa_version":"Published Version","article_number":"koaf006","abstract":[{"lang":"eng","text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. "}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"volume":37,"quality_controlled":"1","date_created":"2025-02-05T06:52:06Z","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"external_id":{"isi":["001462763100001"],"pmid":["39792900"]},"corr_author":"1","language":[{"iso":"eng"}],"publication_status":"published","pmid":1,"acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","file":[{"success":1,"access_level":"open_access","relation":"main_file","file_size":53904111,"file_name":"2025_PlantCell_Gallei.pdf","creator":"dernst","file_id":"20092","date_created":"2025-07-31T07:03:43Z","date_updated":"2025-07-31T07:03:43Z","content_type":"application/pdf","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3"}],"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"oa":1,"author":[{"full_name":"Gallei, Michelle C","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","orcid":"0000-0003-1286-7368"},{"full_name":"Truckenbrodt, Sven M","last_name":"Truckenbrodt","first_name":"Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline"},{"last_name":"Inumella","full_name":"Inumella, Syamala","first_name":"Syamala","id":"F8660870-D756-11E9-98C5-34DFE5697425","orcid":"0009-0002-5890-120X"},{"id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","first_name":"Vitali","last_name":"Vistunou","full_name":"Vistunou, Vitali"},{"orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","last_name":"Sommer","first_name":"Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-7667-6854","first_name":"Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","last_name":"Tavakoli","full_name":"Tavakoli, Mojtaba"},{"last_name":"Agudelo Duenas","full_name":"Agudelo Duenas, Nathalie","first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0009-0000-7590-3501","full_name":"Vorlaufer, Jakob","last_name":"Vorlaufer","first_name":"Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425"},{"first_name":"Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","last_name":"Jahr"},{"first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","full_name":"Randuch, Marek"},{"orcid":"0000-0002-2739-8843","first_name":"Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","full_name":"Johnson, Alexander J"},{"last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml"},{"orcid":"0000-0001-8559-3973","last_name":"Danzl","full_name":"Danzl, Johann G","first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2025-07-31T07:03:43Z","publication":"The Plant Cell","scopus_import":"1","isi":1,"intvolume":" 37","ec_funded":1},{"publication":"Plant and Cell Physiology","scopus_import":"1","author":[{"orcid":"0000-0001-6152-6637","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","first_name":"Han","full_name":"Tang, Han","last_name":"Tang"},{"first_name":"L","last_name":"Chen","full_name":"Chen, L"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"isi":1,"ec_funded":1,"pmid":1,"acknowledgement":"The authors sincerely thank Dr Barbara Kloeckener Gruissem’s time and efforts in critical reading and constructive advice on the manuscript. The authors gratefully acknowledge Dr. Eva Sundberg for generously providing transgenic plants to support this study.\r\nThis work was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.) and the Taiwan National Science and Technology Council (NSTC 112-2311-B-005-008 to H.T. and L.-H.C.).","publication_identifier":{"eissn":["1471-9053"],"issn":["0032-0781"]},"publication_status":"published","date_created":"2025-03-19T09:44:19Z","department":[{"_id":"JiFr"}],"external_id":{"pmid":["39829340"],"isi":["001436802900001"]},"corr_author":"1","language":[{"iso":"eng"}],"quality_controlled":"1","article_number":"pcaf008","abstract":[{"lang":"eng","text":"Auxin and its PIN-FORMED (PIN) exporters are essential for tissue repair and regeneration in flowering plants. To gain insight into the evolution of this mechanism, we investigated their roles in leaves excised from Physcomitrium patens, a bryophyte known for its remarkable cell reprogramming capacity. We used various approaches to manipulate auxin levels, including exogenous application, pharmacological manipulations, and auxin biosynthesis mutants. We observed no significant effect on the rate of cell reprogramming. Rather, our analysis of auxin dynamics revealed a decrease in auxin levels upon excision, which was followed by a local increase before the reprogramming process began. Mutant analysis revealed that PpPINs are required for effective cell reprogramming, and endogenously expressed PpPINA-GFP accumulates polarly at sites that will develop into future filamentous stem cells. In addition, hyperpolarized PpPINA variants carrying mutated phosphorylation sites showed a marked delay in reprogramming, whereas endogenous or nonpolar versions do not have this effect. These results underscore that both the levels and the polarity of PpPINA are important for efficient cell reprogramming. Overall, these findings highlight the pivotal role of PIN polarity in plant regeneration. Furthermore, they suggest that understanding polarity mechanisms could have broader implications for improving regenerative processes across various plant species."}],"date_published":"2025-03-05T00:00:00Z","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"}],"date_updated":"2025-09-30T11:05:55Z","oa_version":"None","year":"2025","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","type":"journal_article","publisher":"Oxford University Press","day":"05","OA_type":"closed access","article_type":"original","doi":"10.1093/pcp/pcaf008","_id":"19420","month":"03","title":"Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.","article_processing_charge":"No","citation":{"short":"H. Tang, L. Chen, J. Friml, Plant and Cell Physiology (2025).","mla":"Tang, Han, et al. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology, pcaf008, Oxford University Press, 2025, doi:10.1093/pcp/pcaf008.","ieee":"H. Tang, L. Chen, and J. Friml, “Auxin fluctuation and PIN polarization in moss leaf cell reprogramming.,” Plant and Cell Physiology. Oxford University Press, 2025.","ama":"Tang H, Chen L, Friml J. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. 2025. doi:10.1093/pcp/pcaf008","apa":"Tang, H., Chen, L., & Friml, J. (2025). Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcaf008","chicago":"Tang, Han, L Chen, and Jiří Friml. “Auxin Fluctuation and PIN Polarization in Moss Leaf Cell Reprogramming.” Plant and Cell Physiology. Oxford University Press, 2025. https://doi.org/10.1093/pcp/pcaf008.","ista":"Tang H, Chen L, Friml J. 2025. Auxin fluctuation and PIN polarization in moss leaf cell reprogramming. Plant and Cell Physiology., pcaf008."}},{"article_processing_charge":"No","citation":{"ieee":"G. Jia et al., “Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize,” Nature Plants, vol. 11. Springer Nature, 2025.","mla":"Jia, G., et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants, vol. 11, 5207, Springer Nature, 2025, doi:10.1038/s41477-025-01934-w.","short":"G. Jia, G. Chen, Z. Zhang, C. Tian, Y. Wang, J. Luo, K. Zhang, X. Zhao, X. Zhao, Z. Li, L. Sun, W. Yang, Y. Guo, J. Friml, Z. Gong, J. Zhang, Nature Plants 11 (2025).","chicago":"Jia, G, G Chen, Z Zhang, C Tian, Y Wang, J Luo, K Zhang, et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants. Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01934-w.","ista":"Jia G, Chen G, Zhang Z, Tian C, Wang Y, Luo J, Zhang K, Zhao X, Zhao X, Li Z, Sun L, Yang W, Guo Y, Friml J, Gong Z, Zhang J. 2025. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 11, 5207.","ama":"Jia G, Chen G, Zhang Z, et al. Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. 2025;11. doi:10.1038/s41477-025-01934-w","apa":"Jia, G., Chen, G., Zhang, Z., Tian, C., Wang, Y., Luo, J., … Zhang, J. (2025). Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01934-w"},"_id":"19422","month":"03","title":"Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize","article_type":"original","OA_type":"green","doi":"10.1038/s41477-025-01934-w","OA_place":"repository","ddc":["580"],"type":"journal_article","day":"05","publisher":"Springer Nature","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","date_published":"2025-03-05T00:00:00Z","date_updated":"2025-11-12T07:52:06Z","year":"2025","oa_version":"Submitted Version","article_number":"5207","abstract":[{"text":"Nitrogen (N) is an essential macronutrient for plant development and, ultimately, yield. Identifying the genetic components and mechanisms underlying N use efficiency in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred inorganic N source in maize. Here we performed a genome-wide association study of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions, identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait. ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction limits their ability to associate with ZmNiRs and stimulate their activity. Knockout lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation, and field experiments show consistently improved N utilization and grain yield under N-deficient conditions. Our work thus provides molecular and mechanistic insights into the natural variation in N utilization, instrumental for genetic improvement of yield in maize and, potentially, in other crops.","lang":"eng"}],"volume":11,"quality_controlled":"1","date_created":"2025-03-19T09:44:55Z","external_id":{"isi":["001437953800001"],"pmid":["40044942"]},"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"publication_status":"published","pmid":1,"acknowledgement":"We thank X. Yang for providing published inbred lines and helping with data analysis; and S. Huang, C. Jiang, G. Bi, C. Liu and S. Zhang for helpful discussions. The transgenic maize lines were generated by the Center for Crop Functional Genomics and Molecular Breeding of China Agricultural University. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500 to J.Z.), the National Natural Science Foundation of China (32170265 and 32441022 to J.Z.), the Chinese Universities Scientific Fund (2024TC084 to J.Z.), the Pinduoduo-China Agricultural University Research Fund (PC2024B01005 to J.Z.), the Hainan Provincial Natural Science Foundation of China (323CXTD379 to J.Z.), and the Central Guidance on Local Science and Technology Development Fund of Shanxi Province (YDZJSX2024D040 to C.T. and J.Z.).","file":[{"file_name":"2025_NaturePlants_Jia_submitted.pdf","creator":"dernst","file_id":"20634","date_created":"2025-11-12T07:50:45Z","file_size":2714177,"checksum":"caeaf1a8bc3e1435e8c995d1d9df5390","content_type":"application/pdf","date_updated":"2025-11-12T07:50:45Z","success":1,"access_level":"open_access","relation":"main_file"}],"oa":1,"publication_identifier":{"issn":["2055-0278"]},"author":[{"last_name":"Jia","full_name":"Jia, G","first_name":"G"},{"first_name":"G","full_name":"Chen, G","last_name":"Chen"},{"first_name":"Z","full_name":"Zhang, Z","last_name":"Zhang"},{"first_name":"C","last_name":"Tian","full_name":"Tian, C"},{"full_name":"Wang, Y","last_name":"Wang","first_name":"Y"},{"first_name":"J","last_name":"Luo","full_name":"Luo, J"},{"first_name":"K","last_name":"Zhang","full_name":"Zhang, K"},{"first_name":"X","last_name":"Zhao","full_name":"Zhao, X"},{"first_name":"X","full_name":"Zhao, X","last_name":"Zhao"},{"first_name":"Z","last_name":"Li","full_name":"Li, Z"},{"last_name":"Sun","full_name":"Sun, L","first_name":"L"},{"first_name":"W","last_name":"Yang","full_name":"Yang, W"},{"last_name":"Guo","full_name":"Guo, Y","first_name":"Y"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"last_name":"Gong","full_name":"Gong, Z","first_name":"Z"},{"last_name":"Zhang","full_name":"Zhang, J","first_name":"J"}],"file_date_updated":"2025-11-12T07:50:45Z","scopus_import":"1","publication":"Nature Plants","isi":1,"intvolume":" 11"},{"abstract":[{"lang":"eng","text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes."}],"volume":246,"quality_controlled":"1","department":[{"_id":"JiFr"}],"external_id":{"pmid":["40047465"],"isi":["001438711600001"]},"date_created":"2025-03-19T09:45:11Z","language":[{"iso":"eng"}],"publication_status":"published","acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","file":[{"checksum":"861c9bf47e7a7766ed03e6d85bd4f6dc","content_type":"application/pdf","date_updated":"2025-04-16T08:03:36Z","creator":"dernst","file_name":"2025_NewPhytologist_Kurtovic.pdf","date_created":"2025-04-16T08:03:36Z","file_id":"19571","file_size":12841729,"access_level":"open_access","relation":"main_file","success":1}],"pmid":1,"publication_identifier":{"issn":["1469-8137"]},"oa":1,"author":[{"last_name":"Kurtović","full_name":"Kurtović, K","first_name":"K"},{"first_name":"S","last_name":"Vosolsobě","full_name":"Vosolsobě, S"},{"first_name":"D","last_name":"Nedvěd","full_name":"Nedvěd, D"},{"first_name":"K","last_name":"Müller","full_name":"Müller, K"},{"first_name":"PI","last_name":"Dobrev","full_name":"Dobrev, PI"},{"full_name":"Schmidt, V","last_name":"Schmidt","first_name":"V"},{"last_name":"Piszczek","full_name":"Piszczek, P","first_name":"P"},{"first_name":"A","last_name":"Kuhn","full_name":"Kuhn, A"},{"id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana","full_name":"Smoljan, Adrijana","last_name":"Smoljan"},{"first_name":"TJ","full_name":"Fisher, TJ","last_name":"Fisher"},{"last_name":"Weijers","full_name":"Weijers, D","first_name":"D"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"JL","full_name":"Bowman, JL","last_name":"Bowman"},{"first_name":"J","full_name":"Petrášek, J","last_name":"Petrášek"}],"scopus_import":"1","file_date_updated":"2025-04-16T08:03:36Z","publication":"New Phytologist","intvolume":" 246","isi":1,"article_processing_charge":"Yes (via OA deal)","citation":{"ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 2025;246(3):1066-1083. doi:10.1111/nph.70019","apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. Wiley. https://doi.org/10.1111/nph.70019","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist. Wiley, 2025. https://doi.org/10.1111/nph.70019.","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist, vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:10.1111/nph.70019.","short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist 246 (2025) 1066–1083.","ieee":"K. Kurtović et al., “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” New Phytologist, vol. 246, no. 3. Wiley, pp. 1066–1083, 2025."},"_id":"19423","title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","month":"05","OA_place":"publisher","doi":"10.1111/nph.70019","article_type":"original","OA_type":"hybrid","ddc":["580"],"type":"journal_article","day":"01","publisher":"Wiley","issue":"3","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","page":"1066-1083","has_accepted_license":"1","project":[{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"date_published":"2025-05-01T00:00:00Z","year":"2025","oa_version":"Published Version","date_updated":"2025-09-30T11:11:18Z"},{"date_published":"2025-05-19T00:00:00Z","date_updated":"2025-09-30T12:41:30Z","year":"2025","oa_version":"None","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","day":"19","publisher":"Springer Nature","type":"journal_article","article_type":"review","OA_type":"closed access","doi":"10.1038/s41580-025-00851-2","_id":"19736","month":"05","title":"Mechanisms of auxin action in plant growth and development","article_processing_charge":"No","citation":{"chicago":"Vanneste, Steffen, Yuanrong Pei, and Jiří Friml. “Mechanisms of Auxin Action in Plant Growth and Development.” Nature Reviews Molecular Cell Biology. Springer Nature, 2025. https://doi.org/10.1038/s41580-025-00851-2.","ista":"Vanneste S, Pei Y, Friml J. 2025. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology., e113018.","ama":"Vanneste S, Pei Y, Friml J. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology. 2025. doi:10.1038/s41580-025-00851-2","apa":"Vanneste, S., Pei, Y., & Friml, J. (2025). Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology. Springer Nature. https://doi.org/10.1038/s41580-025-00851-2","ieee":"S. Vanneste, Y. Pei, and J. Friml, “Mechanisms of auxin action in plant growth and development,” Nature Reviews Molecular Cell Biology. Springer Nature, 2025.","mla":"Vanneste, Steffen, et al. “Mechanisms of Auxin Action in Plant Growth and Development.” Nature Reviews Molecular Cell Biology, e113018, Springer Nature, 2025, doi:10.1038/s41580-025-00851-2.","short":"S. Vanneste, Y. Pei, J. Friml, Nature Reviews Molecular Cell Biology (2025)."},"author":[{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"first_name":"Yuanrong","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","full_name":"Pei, Yuanrong","last_name":"Pei"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml"}],"scopus_import":"1","publication":"Nature Reviews Molecular Cell Biology","isi":1,"pmid":1,"publication_identifier":{"eissn":["1471-0080"],"issn":["1471-0072"]},"publication_status":"published","date_created":"2025-05-25T22:16:57Z","external_id":{"pmid":["40389696"],"isi":["001490500500001"]},"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"corr_author":"1","quality_controlled":"1","article_number":"e113018","abstract":[{"lang":"eng","text":"The phytohormone auxin is a major signal coordinating growth and development in plants. The variety of its effects arises from its ability to form local auxin maxima and gradients within tissues, generated through directional cell-to-cell transport and elaborate metabolic control. These auxin distribution patterns instruct cells in a context-dependent manner to undergo predefined developmental transitions. In this Review, we discuss advances in auxin action at the level of homeostasis and signalling. We highlight key insights into the structural basis of PIN-mediated intercellular auxin transport and explore two novel non-transcriptional auxin signalling mechanisms: one involving intracellular Ca2+ transients and another involving cell-surface auxin perception that mediates global, ultrafast phosphorylation. Furthermore, we examine emerging evidence indicating the involvement of cyclic adenosine monophosphate as a second messenger in the transcriptional auxin response. Together, these recent developments in auxin research have profoundly deepened our understanding of the complex and diverse activities of auxin in plant growth and development."}]},{"acknowledgement":"We gratefully acknowledge the funding by the Austrian Science Fund (FWF; I 6123-B and P 37051-B) and the European Research Council (ERC; 101142681 CYNIPS).We would like to thank Lukas Fiedler for his significant input and thoughtful revision of this manuscript.","file":[{"date_updated":"2026-02-10T09:35:43Z","checksum":"6c190faacf0e3bef98311dc8a12132d4","content_type":"application/pdf","file_size":974106,"creator":"dernst","file_name":"2025_NPJSciencePlants_Monzer.pdf","date_created":"2026-02-10T09:35:43Z","file_id":"21208","access_level":"open_access","relation":"main_file","success":1}],"pmid":1,"oa":1,"publication_identifier":{"eissn":["3005-1401"]},"publication":"npj Science of Plants","author":[{"first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","last_name":"Monzer"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"file_date_updated":"2026-02-10T09:35:43Z","intvolume":" 1","volume":1,"quality_controlled":"1","abstract":[{"text":"The plant hormone auxin regulates growth and development through at least two distinct signaling pathways. The nuclear pathway, involving TIR1/AFB receptors, mediates transcription; whereas the cell surface ABP1-TMK1 auxin perception triggers global ultrafast phosphorylation response. Here, we revisit the rich history of the disputed ABP1 auxin receptor, highlighting recent findings of the involvement of TMKs and other molecular components and focusing on their role in auxin canalization-mediated development.","lang":"eng"}],"publication_status":"published","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"external_id":{"pmid":["40630787"]},"date_created":"2026-02-03T13:03:53Z","language":[{"iso":"eng"}],"corr_author":"1","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"type":"journal_article","day":"01","publisher":"Springer Nature","issue":"1","date_published":"2025-07-01T00:00:00Z","has_accepted_license":"1","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"},{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"}],"oa_version":"Published Version","year":"2025","date_updated":"2026-02-10T09:39:20Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"2","_id":"21136","title":"Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.","month":"07","article_processing_charge":"Yes (in subscription journal)","citation":{"short":"A. Monzer, J. Friml, Npj Science of Plants 1 (2025) 2.","mla":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants, vol. 1, no. 1, Springer Nature, 2025, p. 2, doi:10.1038/s44383-025-00002-8.","ieee":"A. Monzer and J. Friml, “Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.,” npj Science of Plants, vol. 1, no. 1. Springer Nature, p. 2, 2025.","ama":"Monzer A, Friml J. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 2025;1(1):2. doi:10.1038/s44383-025-00002-8","apa":"Monzer, A., & Friml, J. (2025). Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. Npj Science of Plants. Springer Nature. https://doi.org/10.1038/s44383-025-00002-8","chicago":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” Npj Science of Plants. Springer Nature, 2025. https://doi.org/10.1038/s44383-025-00002-8.","ista":"Monzer A, Friml J. 2025. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 1(1), 2."},"ddc":["580"],"doi":"10.1038/s44383-025-00002-8","OA_place":"publisher","OA_type":"hybrid","article_type":"original"},{"page":"299-322","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Cryptic Enzymes and Moonlighting Proteins","author":[{"first_name":"Linlin","last_name":"Qi","full_name":"Qi, Linlin"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"}],"scopus_import":"1","date_updated":"2026-02-17T13:28:38Z","year":"2025","oa_version":"None","date_published":"2025-05-02T00:00:00Z","publisher":"Elsevier","day":"02","type":"book_chapter","publication_identifier":{"isbn":["9780443157196"]},"status":"public","alternative_title":["Foundations and Frontiers in Enzymology"],"OA_type":"closed access","language":[{"iso":"eng"}],"doi":"10.1016/b978-0-443-15719-6.00015-5","date_created":"2026-02-16T15:53:52Z","department":[{"_id":"JiFr"}],"publication_status":"published","editor":[{"last_name":"Irving","full_name":"Irving, Helen","first_name":"Helen"},{"last_name":"Gehring","full_name":"Gehring, Chris","first_name":"Chris"},{"full_name":"Wong, Aloysius","last_name":"Wong","first_name":"Aloysius"}],"citation":{"short":"L. Qi, J. Friml, in:, H. Irving, C. Gehring, A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins, Elsevier, 2025, pp. 299–322.","mla":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving et al., Elsevier, 2025, pp. 299–322, doi:10.1016/b978-0-443-15719-6.00015-5.","ieee":"L. Qi and J. Friml, “Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling,” in Cryptic Enzymes and Moonlighting Proteins, H. Irving, C. Gehring, and A. Wong, Eds. Elsevier, 2025, pp. 299–322.","apa":"Qi, L., & Friml, J. (2025). Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In H. Irving, C. Gehring, & A. Wong (Eds.), Cryptic Enzymes and Moonlighting Proteins (pp. 299–322). Elsevier. https://doi.org/10.1016/b978-0-443-15719-6.00015-5","ama":"Qi L, Friml J. Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Irving H, Gehring C, Wong A, eds. Cryptic Enzymes and Moonlighting Proteins. Elsevier; 2025:299-322. doi:10.1016/b978-0-443-15719-6.00015-5","ista":"Qi L, Friml J. 2025.Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling. In: Cryptic Enzymes and Moonlighting Proteins. Foundations and Frontiers in Enzymology, , 299–322.","chicago":"Qi, Linlin, and Jiří Friml. “Nucleotidyl Cyclase Activities of TIR1/AFB Auxin Receptors: New Insights into the Mechanism of Auxin Signaling.” In Cryptic Enzymes and Moonlighting Proteins, edited by Helen Irving, Chris Gehring, and Aloysius Wong, 299–322. Elsevier, 2025. https://doi.org/10.1016/b978-0-443-15719-6.00015-5."},"article_processing_charge":"No","abstract":[{"lang":"eng","text":"As an important plant hormone to regulate growth and development, auxin has been investigated for more than a century. It had been clearly demonstrated and well-accepted that the intracellular auxin receptors, TIR1/AFBs, are F-box proteins mediating transcriptional auxin signaling by their E3 ubiquitin ligase activity, which targets and sends for degradation the Aux/IAA transcriptional repressors. The recent discovery of adenylate cyclase (AC) and guanylate cyclase (GC) activities for TIR1/AFBs open entirely new perspectives on how auxin signaling can operate. This chapter traces back the history of how canonical transcriptional auxin signaling was established and introduces the discovery of the TIR1/AFBs-mediated nontranscriptional signaling branch. Finally, the current understanding and open questions of how TIR1/AFBs’ AC and GC activities contribute to the transcriptional and nontranscriptional auxin signaling are discussed, highlighting the possibility that cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) act as second messengers in auxin signal transduction."}],"month":"05","quality_controlled":"1","title":"Nucleotidyl cyclase activities of TIR1/AFB auxin receptors: new insights into the mechanism of auxin signaling","_id":"21255"},{"status":"public","acknowledgement":"This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa":1,"day":"16","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.01.06.631460"}],"type":"preprint","date_published":"2025-05-16T00:00:00Z","date_updated":"2026-04-07T11:41:43Z","year":"2025","oa_version":"Preprint","publication":"bioRxiv","author":[{"full_name":"Kulich, Ivan","last_name":"Kulich","first_name":"Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"first_name":"Denisa","full_name":"Oulehlová, Denisa","last_name":"Oulehlová"},{"full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii"},{"first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","last_name":"Zou"},{"last_name":"Lileikyte","full_name":"Lileikyte, Edita","first_name":"Edita"},{"first_name":"Alexey","last_name":"Bondar","full_name":"Bondar, Alexey"},{"full_name":"Kulichová, Katarína","last_name":"Kulichová","first_name":"Katarína"},{"full_name":"Janda, Martin","last_name":"Janda","first_name":"Martin"},{"full_name":"Iakovenko, Oksana","last_name":"Iakovenko","first_name":"Oksana"},{"last_name":"Neubergerová","full_name":"Neubergerová, Michaela","first_name":"Michaela"},{"full_name":"Studtrucker, Tanja","last_name":"Studtrucker","first_name":"Tanja"},{"last_name":"Pleskot","full_name":"Pleskot, Roman","first_name":"Roman"},{"last_name":"Dietrich","full_name":"Dietrich, Petra","first_name":"Petra"},{"full_name":"Fendrych, Matyas","last_name":"Fendrych","first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","_id":"20982","month":"05","title":"Armadillo repeat only proteins are required for the function of plant CNGC channels","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants."}],"related_material":{"record":[{"id":"20964","status":"public","relation":"dissertation_contains"}]},"citation":{"ieee":"I. Kulich et al., “Armadillo repeat only proteins are required for the function of plant CNGC channels,” bioRxiv. .","mla":"Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, doi:10.1101/2025.01.06.631460.","short":"I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.).","chicago":"Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” BioRxiv, n.d. https://doi.org/10.1101/2025.01.06.631460.","ista":"Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, 10.1101/2025.01.06.631460.","ama":"Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. doi:10.1101/2025.01.06.631460","apa":"Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv. https://doi.org/10.1101/2025.01.06.631460"},"publication_status":"draft","date_created":"2026-01-13T14:07:58Z","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"OA_place":"repository","corr_author":"1","doi":"10.1101/2025.01.06.631460"},{"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.28.640727"}],"acknowledgement":"We deeply appreciate M. Wrzaczek’s constructive input and insightful discussions, which significantly enriched this work. We thank L. Fiedler for helping with the heat map and for the discussions. We also thank the facilities at ISTA, the imaging and optics (IOF) and Lab Support (LSF) facilities for their service and assistance.","oa":1,"publication":"bioRxiv","author":[{"last_name":"Monzer","full_name":"Monzer, Aline","first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425"},{"first_name":"Ewa","full_name":"Mazur, Ewa","last_name":"Mazur"},{"orcid":"0000-0002-7244-7237","full_name":"Rodriguez Solovey, Lesia","last_name":"Rodriguez Solovey","id":"3922B506-F248-11E8-B48F-1D18A9856A87","first_name":"Lesia"},{"orcid":"0000-0003-1286-7368","last_name":"Gallei","full_name":"Gallei, Michelle C","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","last_name":"Zou"},{"id":"79a5a1be-04a3-11f0-ba18-a1730e0b58e9","first_name":"Michael","last_name":"Smejkal","full_name":"Smejkal, Michael"},{"full_name":"Cervenova, Ema","last_name":"Cervenova","id":"9f185b95-04a3-11f0-8245-f5e32eeb470f","first_name":"Ema"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml"}],"abstract":[{"text":"Receptor-like kinases (RLKs), particularly the Transmembrane Kinase (TMK) family, play essential roles in signaling and development, with TMKs being key components of auxin perception and downstream phosphorylation events. While TMKs’ involvement in auxin canalization, a process essential for vasculature formation and regeneration, has been established, nonetheless, the additional signaling and regulatory partners remain poorly understood. In this study, we identify and characterize seven leucine-rich repeat RLKs (TINT1–TINT7) as novel interactors of TMK1, revealing their diverse evolutionary, structural, and functional characteristics. Our results show that TINTs interact with TMK1 and highlight their roles in regulating various developmental processes. Majority of TINTs contributes, together with TMK1, to auxin canalization, with TINT5 linking TMK1 to other canalization component CAMEL. Beyond canalization, we also establish the role of TINT-TMK1 interactions in processes such as stomatal movement and the hypocotyl’s gravitropic response. These findings suggest that TINTs, through their interaction with TMK1, are integral components of various signaling networks, contributing to both auxin canalization and broader plant development.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"date_created":"2025-03-12T14:28:53Z","department":[{"_id":"GradSch"},{"_id":"JiFr"},{"_id":"EvBe"}],"corr_author":"1","language":[{"iso":"eng"}],"publication_status":"draft","type":"preprint","day":"02","publisher":"Cold Spring Harbor Laboratory","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-03-02T00:00:00Z","has_accepted_license":"1","date_updated":"2026-04-07T11:48:31Z","year":"2025","oa_version":"Published Version","article_processing_charge":"No","related_material":{"record":[{"id":"19395","relation":"dissertation_contains","status":"public"}]},"citation":{"ieee":"A. Monzer et al., “TMK interacting network of receptor like kinases for auxin canalization and beyond,” bioRxiv. Cold Spring Harbor Laboratory.","mla":"Monzer, Aline, et al. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” BioRxiv, Cold Spring Harbor Laboratory, doi:10.1101/2025.02.28.640727.","short":"A. Monzer, E. Mazur, L. Rodriguez Solovey, M.C. Gallei, M. Zou, M. Smejkal, E. Cervenova, J. Friml, BioRxiv (n.d.).","ista":"Monzer A, Mazur E, Rodriguez Solovey L, Gallei MC, Zou M, Smejkal M, Cervenova E, Friml J. TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv, 10.1101/2025.02.28.640727.","chicago":"Monzer, Aline, Ewa Mazur, Lesia Rodriguez Solovey, Michelle C Gallei, Minxia Zou, Michael Smejkal, Ema Cervenova, and Jiří Friml. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” BioRxiv. Cold Spring Harbor Laboratory, n.d. https://doi.org/10.1101/2025.02.28.640727.","apa":"Monzer, A., Mazur, E., Rodriguez Solovey, L., Gallei, M. C., Zou, M., Smejkal, M., … Friml, J. (n.d.). TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2025.02.28.640727","ama":"Monzer A, Mazur E, Rodriguez Solovey L, et al. TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv. doi:10.1101/2025.02.28.640727"},"_id":"19398","month":"03","title":"TMK interacting network of receptor like kinases for auxin canalization and beyond","OA_type":"green","OA_place":"repository","doi":"10.1101/2025.02.28.640727"},{"month":"08","title":"Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development","_id":"20187","related_material":{"record":[{"id":"20362","relation":"dissertation_contains","status":"public"}]},"PlanS_conform":"1","citation":{"ieee":"D. Babic et al., “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant Journal, vol. 123, no. 3. Wiley, 2025.","mla":"Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396.","short":"D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).","chicago":"Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396.","ista":"Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396.","ama":"Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396","apa":"Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. Wiley. https://doi.org/10.1111/tpj.70396"},"article_processing_charge":"Yes (via OA deal)","ddc":["580"],"article_type":"original","OA_type":"hybrid","OA_place":"publisher","doi":"10.1111/tpj.70396","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","issue":"3","day":"01","type":"journal_article","publisher":"Wiley","date_updated":"2026-04-07T11:52:02Z","oa_version":"Published Version","year":"2025","has_accepted_license":"1","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"}],"date_published":"2025-08-01T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","quality_controlled":"1","volume":123,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"article_number":"e70396","abstract":[{"lang":"eng","text":"Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development."}],"publication_status":"published","corr_author":"1","language":[{"iso":"eng"}],"date_created":"2025-08-17T22:01:36Z","external_id":{"pmid":["40782342"],"isi":["001547884300001"]},"department":[{"_id":"EvBe"},{"_id":"JiFr"},{"_id":"GradSch"}],"oa":1,"publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"pmid":1,"acknowledgement":"We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","file":[{"file_id":"20264","date_created":"2025-09-01T14:09:31Z","file_name":"2025_PlantJournal_Babic.pdf","creator":"dernst","file_size":5791111,"checksum":"1cdc3341d2d23101abca72521f1f23cb","content_type":"application/pdf","date_updated":"2025-09-01T14:09:31Z","success":1,"relation":"main_file","access_level":"open_access"}],"intvolume":" 123","isi":1,"file_date_updated":"2025-09-01T14:09:31Z","scopus_import":"1","publication":"Plant Journal","author":[{"full_name":"Babic, David","last_name":"Babic","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","first_name":"David"},{"last_name":"Abualia","full_name":"Abualia, Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87","first_name":"Rashed","orcid":"0000-0002-9357-9415"},{"first_name":"Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler","full_name":"Fiedler, Lukas"},{"orcid":"0000-0001-5187-8401","last_name":"Qi","full_name":"Qi, Linlin","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","first_name":"Linlin"},{"first_name":"Frédérique","full_name":"Tellier, Frédérique","last_name":"Tellier"},{"full_name":"Smoljan, Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana"},{"last_name":"Rakusova","full_name":"Rakusova, Hana","first_name":"Hana","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA"},{"first_name":"Petr","id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87","last_name":"Valošek","full_name":"Valošek, Petr"},{"id":"31435098-F248-11E8-B48F-1D18A9856A87","first_name":"Huibin","full_name":"Han, Huibin","last_name":"Han"},{"orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva"},{"first_name":"Jean Denis","last_name":"Faure","full_name":"Faure, Jean Denis"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"}]}]