---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19421'
abstract:
- lang: eng
text: The phytohormone auxin (Aux) is a principal endogenous developmental signal
in plants. It mediates transcriptional reprogramming by a well-established canonical
signalling mechanism. TIR1/AFB auxin receptors are F-box subunits of an ubiquitin
ligase complex; after auxin perception, they associate with Aux/IAA transcriptional
repressors and ubiquitinate them for degradation, thus enabling the activation
of auxin response factor (ARF) transcription factors1,2,3. Here we revise this
paradigm by showing that without TIR1 adenylate cyclase (AC) activity4, auxin-induced
degradation of Aux/IAAs is not sufficient to mediate the transcriptional auxin
response. Abolishing the TIR1 AC activity does not affect auxin-induced degradation
of Aux/IAAs but renders TIR1 non-functional in mediating transcriptional reprogramming
and auxin-regulated development, including shoot, root, root hair growth and lateral
root formation. Transgenic plants show that local cAMP production in the vicinity
of the Aux/IAA–ARF complex by unrelated AC enzymes bypasses the need for auxin
perception and is sufficient to induce ARF-mediated transcription. These discoveries
revise the canonical model of auxin signalling and establish TIR1/AFB-produced
cAMP as a second messenger essential for transcriptional reprograming.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: We are grateful to J. Callis and H.-Q. Yang for sharing materials
and to M. Estelle and S. Kepinski for inspiring discussions. This research was supported
by the Laboratory Support Facility, the Plant Facility and the Imaging and Optics
Facility of the Institute of Science and Technology Austria. This project has received
funding from the European Research Council (101142681 CYNIPS) and Austrian Science
Fund (P 37051-B). L.Q. was supported by the National Natural Science Foundation
of China (grant no. 32470327). M.Z. was supported by the Interdisciplinary Project
Committee of the Institute of Science and Technology Austria, and Y.P. was supported
by an EMBO Postdoctoral Fellowship (ALTF 38-2023). Open access funding provided
by Institute of Science and Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Mengting
full_name: Lu, Mengting
id: a8198a14-1ffe-11ee-8b67-d2bdff9d9178
last_name: Lu
- first_name: M
full_name: Kwiatkowski, M
last_name: Kwiatkowski
- first_name: Yuanrong
full_name: Pei, Yuanrong
id: 98605edc-6ce7-11ee-95f3-cc16b866efcd
last_name: Pei
- first_name: K
full_name: Jaworski, K
last_name: Jaworski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Chen H, Qi L, Zou M, et al. TIR1-produced cAMP as a second messenger in transcriptional
auxin signalling. Nature. 2025;640:1011-1016. doi:10.1038/s41586-025-08669-w
apa: Chen, H., Qi, L., Zou, M., Lu, M., Kwiatkowski, M., Pei, Y., … Friml, J. (2025).
TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
Nature. Springer Nature. https://doi.org/10.1038/s41586-025-08669-w
chicago: Chen, Huihuang, Linlin Qi, Minxia Zou, Mengting Lu, M Kwiatkowski, Yuanrong
Pei, K Jaworski, and Jiří Friml. “TIR1-Produced CAMP as a Second Messenger in
Transcriptional Auxin Signalling.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-025-08669-w.
ieee: H. Chen et al., “TIR1-produced cAMP as a second messenger in transcriptional
auxin signalling,” Nature, vol. 640. Springer Nature, pp. 1011–1016, 2025.
ista: Chen H, Qi L, Zou M, Lu M, Kwiatkowski M, Pei Y, Jaworski K, Friml J. 2025.
TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
Nature. 640, 1011–1016.
mla: Chen, Huihuang, et al. “TIR1-Produced CAMP as a Second Messenger in Transcriptional
Auxin Signalling.” Nature, vol. 640, Springer Nature, 2025, pp. 1011–16,
doi:10.1038/s41586-025-08669-w.
short: H. Chen, L. Qi, M. Zou, M. Lu, M. Kwiatkowski, Y. Pei, K. Jaworski, J. Friml,
Nature 640 (2025) 1011–1016.
corr_author: '1'
date_created: 2025-03-19T09:44:39Z
date_published: 2025-04-24T00:00:00Z
date_updated: 2026-04-28T13:42:45Z
day: '24'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-025-08669-w
external_id:
isi:
- '001437493900001'
pmid:
- '40044868'
file:
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checksum: f5f18081003e7a1b8e372ecb7da82e7d
content_type: application/pdf
creator: dernst
date_created: 2025-08-05T12:29:35Z
date_updated: 2025-08-05T12:29:35Z
file_id: '20132'
file_name: 2025_Nature_Chen.pdf
file_size: 13549245
relation: main_file
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file_date_updated: 2025-08-05T12:29:35Z
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intvolume: ' 640'
isi: 1
language:
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month: '04'
oa: 1
oa_version: Published Version
page: 1011-1016
pmid: 1
project:
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/updating-the-textbook/
record:
- id: '19478'
relation: dissertation_contains
status: public
status: public
title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 640
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20187'
abstract:
- lang: eng
text: Very long-chain fatty acids (VLCFAs), being constituents of different types
of lipids, are critical factors in plant development, presumably due to their
impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic
reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase
1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems
(esm) mutant, a viable kcr1 allele presumably affecting surface properties of
the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that
impacts VLCFAs. The kcr1-2 plants show severe defects during different stages
of development, which all correlate with defects in polar localization and subcellular
trafficking of PIN auxin transporters and resulting asymmetric auxin distribution.
Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests
that KCR1 plays a role in delineating boundaries around meristematic and specialized
differentiating tissues, including root and shoot meristems, initiating lateral
roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced
VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful
tool to study VLCFA roles in plant development and highlights VLCFAs as critical
developmental factors at the interface of cell polarity and tissue development.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy
(especially Vanessa Zheden for technical assistance) and Life Science (in particular
Dorota Jaworska) facilities at ISTA for their continuous support. Authors would
like to thank Michelle Gallei for advice during the generation of the transgenic
lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help
and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and
root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski
for advice on abiotic stress experiments. We would like to thank Dolf Weijers for
the SOSEKI (SOK) marker line seeds. This work has benefited from the support of
IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian
Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province
(20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant
Sciences-SPS (ANR-17-EUR-0007)."
article_number: e70396
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: David
full_name: Babic, David
id: db566d23-f6e0-11ea-865d-e6f270e968e7
last_name: Babic
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Frédérique
full_name: Tellier, Frédérique
last_name: Tellier
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Hana
full_name: Rakusova, Hana
id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
last_name: Rakusova
- first_name: Petr
full_name: Valošek, Petr
id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
last_name: Valošek
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jean Denis
full_name: Faure, Jean Denis
last_name: Faure
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty
acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396
apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml,
J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis
auxin-mediated embryonic and post-embryonic development. Plant Journal.
Wiley. https://doi.org/10.1111/tpj.70396
chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier,
Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty
Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic
Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396.
ieee: D. Babic et al., “Biosynthesis of very long-chain fatty acids is required
for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant
Journal, vol. 123, no. 3. Wiley, 2025.
ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek
P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain
fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 123(3), e70396.
mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required
for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant
Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396.
short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova,
P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).
corr_author: '1'
date_created: 2025-08-17T22:01:36Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-04-07T11:52:02Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
- _id: GradSch
doi: 10.1111/tpj.70396
external_id:
isi:
- '001547884300001'
pmid:
- '40782342'
file:
- access_level: open_access
checksum: 1cdc3341d2d23101abca72521f1f23cb
content_type: application/pdf
creator: dernst
date_created: 2025-09-01T14:09:31Z
date_updated: 2025-09-01T14:09:31Z
file_id: '20264'
file_name: 2025_PlantJournal_Babic.pdf
file_size: 5791111
relation: main_file
success: 1
file_date_updated: 2025-09-01T14:09:31Z
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intvolume: ' 123'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: Plant Journal
publication_identifier:
eissn:
- 1365-313X
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
- id: '20362'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated
embryonic and post-embryonic development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 123
year: '2025'
...
---
DOAJ_listed: '1'
_id: '15257'
abstract:
- lang: eng
text: Root gravitropic bending represents a fundamental aspect of terrestrial plant
physiology. Gravity is perceived by sedimentation of starch-rich plastids (statoliths)
to the bottom of the central root cap cells. Following gravity perception, intercellular
auxin transport is redirected downwards leading to an asymmetric auxin accumulation
at the lower root side causing inhibition of cell expansion, ultimately resulting
in downwards bending. How gravity-induced statoliths repositioning is translated
into asymmetric auxin distribution remains unclear despite PIN auxin efflux carriers
and the Negative Gravitropic Response of roots (NGR) proteins polarize along statolith
sedimentation, thus providing a plausible mechanism for auxin flow redirection.
In this study, using a functional NGR1-GFP construct, we visualized the NGR1 localization
on the statolith surface and plasma membrane (PM) domains in close proximity to
the statoliths, correlating with their movements. We determined that NGR1 binding
to these PM domains is indispensable for NGR1 functionality and relies on cysteine
acylation and adjacent polybasic regions as well as on lipid and sterol PM composition.
Detailed timing of the early events following graviperception suggested that both
NGR1 repolarization and initial auxin asymmetry precede the visible PIN3 polarization.
This discrepancy motivated us to unveil a rapid, NGR-dependent translocation of
PIN-activating AGCVIII kinase D6PK towards lower PMs of gravity-perceiving cells,
thus providing an attractive model for rapid redirection of auxin fluxes following
gravistimulation.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: 'The research leading to these results has received funding from
the European Research Council (ERC) under the European Union’s Horizon 2020 research
and innovation programme grant agreement No 742985 and Austrian Science Fund (FWF):
I3630-775 B25 to J.F. This research was also supported by the Lab Support Facility
(LSF) and the Imaging and Optics Facility (IOF) of IST Austria, namely Tereza Bělinová
for her help with the imaging. JS was supported by FemTECH fellowship.'
article_number: '91523'
article_processing_charge: Yes
article_type: original
author:
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Julia
full_name: Schmid, Julia
id: 07cf4637-baaf-11ee-9227-e1de57d1d69b
last_name: Schmid
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kulich I, Schmid J, Teplova A, Qi L, Friml J. Rapid translocation of NGR proteins
driving polarization of PIN-activating D6 protein kinase during root gravitropism.
eLife. 2024;12. doi:10.7554/elife.91523
apa: Kulich, I., Schmid, J., Teplova, A., Qi, L., & Friml, J. (2024). Rapid
translocation of NGR proteins driving polarization of PIN-activating D6 protein
kinase during root gravitropism. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.91523
chicago: Kulich, Ivan, Julia Schmid, Anastasiia Teplova, Linlin Qi, and Jiří Friml.
“Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6
Protein Kinase during Root Gravitropism.” ELife. eLife Sciences Publications,
2024. https://doi.org/10.7554/elife.91523.
ieee: I. Kulich, J. Schmid, A. Teplova, L. Qi, and J. Friml, “Rapid translocation
of NGR proteins driving polarization of PIN-activating D6 protein kinase during
root gravitropism,” eLife, vol. 12. eLife Sciences Publications, 2024.
ista: Kulich I, Schmid J, Teplova A, Qi L, Friml J. 2024. Rapid translocation of
NGR proteins driving polarization of PIN-activating D6 protein kinase during root
gravitropism. eLife. 12, 91523.
mla: Kulich, Ivan, et al. “Rapid Translocation of NGR Proteins Driving Polarization
of PIN-Activating D6 Protein Kinase during Root Gravitropism.” ELife, vol.
12, 91523, eLife Sciences Publications, 2024, doi:10.7554/elife.91523.
short: I. Kulich, J. Schmid, A. Teplova, L. Qi, J. Friml, ELife 12 (2024).
corr_author: '1'
date_created: 2024-04-02T11:35:58Z
date_published: 2024-03-05T00:00:00Z
date_updated: 2025-04-23T07:45:02Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.7554/elife.91523
ec_funded: 1
external_id:
pmid:
- '38441122'
file:
- access_level: open_access
checksum: a73a84d3bf97a6d09d24308ca6dd0a0c
content_type: application/pdf
creator: dernst
date_created: 2024-04-03T13:18:00Z
date_updated: 2024-04-03T13:18:00Z
file_id: '15288'
file_name: 2024_eLife_Kulich.pdf
file_size: 11451904
relation: main_file
success: 1
file_date_updated: 2024-04-03T13:18:00Z
has_accepted_license: '1'
intvolume: ' 12'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Medicine
- General Neuroscience
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/beneath-the-surface/
scopus_import: '1'
status: public
title: Rapid translocation of NGR proteins driving polarization of PIN-activating
D6 protein kinase during root gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2024'
...
---
_id: '13212'
abstract:
- lang: eng
text: Auxin is the major plant hormone regulating growth and development (Friml,
2022). Forward genetic approaches in the model plant Arabidopsis thaliana have
identified major components of auxin signalling and established the canonical
mechanism mediating transcriptional and thus developmental reprogramming. In this
textbook view, TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFBs)
are auxin receptors, which act as F-box subunits determining the substrate specificity
of the Skp1-Cullin1-F box protein (SCF) type E3 ubiquitin ligase complex. Auxin
acts as a “molecular glue” increasing the affinity between TIR1/AFBs and the Aux/IAA
repressors. Subsequently, Aux/IAAs are ubiquitinated and degraded, thus releasing
auxin transcription factors from their repression making them free to mediate
transcription of auxin response genes (Yu et al., 2022). Nonetheless, accumulating
evidence suggests existence of rapid, non-transcriptional responses downstream
of TIR1/AFBs such as auxin-induced cytosolic calcium (Ca2+) transients, plasma
membrane depolarization and apoplast alkalinisation, all converging on the process
of root growth inhibition and root gravitropism (Li et al., 2022). Particularly,
these rapid responses are mostly contributed by predominantly cytosolic AFB1,
while the long-term growth responses are mediated by mainly nuclear TIR1 and AFB2-AFB5
(Li et al., 2021; Prigge et al., 2020; Serre et al., 2021). How AFB1 conducts
auxin-triggered rapid responses and how it is different from TIR1 and AFB2-AFB5
remains elusive. Here, we compare the roles of TIR1 and AFB1 in transcriptional
and rapid responses by modulating their subcellular localization in Arabidopsis
and by testing their ability to mediate transcriptional responses when part of
the minimal auxin circuit reconstituted in yeast.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: We thank all the authors for sharing the published materials. This
research was supported by the Lab Support Facility and the Imaging and Optics Facility
of ISTA. We thank Lukáš Fiedler (ISTA) for critical reading of the manuscript. This
project was funded by the European Research Council Advanced Grant (ETAP-742985).
article_processing_charge: Yes (via OA deal)
article_type: letter_note
author:
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Chen H, Li L, Zou M, Qi L, Friml J. Distinct functions of TIR1 and AFB1 receptors
in auxin signalling. Molecular Plant. 2023;16(7):1117-1119. doi:10.1016/j.molp.2023.06.007
apa: Chen, H., Li, L., Zou, M., Qi, L., & Friml, J. (2023). Distinct functions
of TIR1 and AFB1 receptors in auxin signalling. Molecular Plant. Elsevier
. https://doi.org/10.1016/j.molp.2023.06.007
chicago: Chen, Huihuang, Lanxin Li, Minxia Zou, Linlin Qi, and Jiří Friml. “Distinct
Functions of TIR1 and AFB1 Receptors in Auxin Signalling.” Molecular Plant.
Elsevier , 2023. https://doi.org/10.1016/j.molp.2023.06.007.
ieee: H. Chen, L. Li, M. Zou, L. Qi, and J. Friml, “Distinct functions of TIR1 and
AFB1 receptors in auxin signalling.,” Molecular Plant, vol. 16, no. 7.
Elsevier , pp. 1117–1119, 2023.
ista: Chen H, Li L, Zou M, Qi L, Friml J. 2023. Distinct functions of TIR1 and AFB1
receptors in auxin signalling. Molecular Plant. 16(7), 1117–1119.
mla: Chen, Huihuang, et al. “Distinct Functions of TIR1 and AFB1 Receptors in Auxin
Signalling.” Molecular Plant, vol. 16, no. 7, Elsevier , 2023, pp. 1117–19,
doi:10.1016/j.molp.2023.06.007.
short: H. Chen, L. Li, M. Zou, L. Qi, J. Friml, Molecular Plant 16 (2023) 1117–1119.
corr_author: '1'
date_created: 2023-07-12T07:32:46Z
date_published: 2023-07-01T00:00:00Z
date_updated: 2026-04-07T11:51:24Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.molp.2023.06.007
ec_funded: 1
external_id:
isi:
- '001044410900001'
pmid:
- '37393433'
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oa_version: Published Version
page: 1117-1119
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call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Molecular Plant
publication_identifier:
eissn:
- 1674-2052
issn:
- 1752-9867
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
related_material:
record:
- id: '19478'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Distinct functions of TIR1 and AFB1 receptors in auxin signalling.
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2023'
...
---
_id: '13266'
abstract:
- lang: eng
text: The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger
in many mammalian signaling pathways. However, its role in plants remains not
well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport
inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors
and the demonstration of its importance for canonical auxin signaling put plant
cAMP research back into spotlight. This insight briefly summarizes the well-established
cAMP signaling pathways in mammalian cells and describes the turbulent and controversial
history of plant cAMP research highlighting the major progress and the unresolved
points. We also briefly review the current paradigm of auxin signaling to provide
a background for the discussion on the AC activity of TIR1/AFB auxin receptors
and its potential role in transcriptional auxin signaling as well as impact of
these discoveries on plant cAMP research in general.
acknowledgement: 'We gratefully acknowledge our brave colleagues, whose excellent
efforts kept the plant cAMP research going in the last two decades. The authors
were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond.
New Phytologist. 2023;240(2):489-495. doi:10.1111/nph.19123
apa: Qi, L., & Friml, J. (2023). Tale of cAMP as a second messenger in auxin
signaling and beyond. New Phytologist. Wiley. https://doi.org/10.1111/nph.19123
chicago: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin
Signaling and Beyond.” New Phytologist. Wiley, 2023. https://doi.org/10.1111/nph.19123.
ieee: L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling
and beyond,” New Phytologist, vol. 240, no. 2. Wiley, pp. 489–495, 2023.
ista: Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling
and beyond. New Phytologist. 240(2), 489–495.
mla: Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling
and Beyond.” New Phytologist, vol. 240, no. 2, Wiley, 2023, pp. 489–95,
doi:10.1111/nph.19123.
short: L. Qi, J. Friml, New Phytologist 240 (2023) 489–495.
corr_author: '1'
date_created: 2023-07-23T22:01:13Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2024-10-22T12:50:00Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.19123
external_id:
isi:
- '001026321500001'
pmid:
- '37434303'
file:
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creator: dernst
date_created: 2024-01-29T11:21:43Z
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file_size: 974464
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file_date_updated: 2024-01-29T11:21:43Z
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intvolume: ' 240'
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language:
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month: '10'
oa: 1
oa_version: Published Version
page: 489-495
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tale of cAMP as a second messenger in auxin signaling and beyond
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 240
year: '2023'
...
---
_id: '12144'
abstract:
- lang: eng
text: The phytohormone auxin is the major coordinative signal in plant development1,
mediating transcriptional reprogramming by a well-established canonical signalling
pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin
receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin,
they associate with Aux/IAA transcriptional repressors and target them for degradation
via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an
additional function of TIR1/AFB receptors across land plants. Auxin, together
with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC
motif of the TIR1 C-terminal region, all of which abolish the AC activity, each
render TIR1 ineffective in mediating gravitropism and sustained auxin-induced
root growth inhibition, and also affect auxin-induced transcriptional regulation.
These results highlight the importance of TIR1/AFB AC activity in canonical auxin
signalling. They also identify a unique phytohormone receptor cassette combining
F-box and AC motifs, and the role of cAMP as a second messenger in plants.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: This research was supported by the Lab Support Facility (LSF) and
the Imaging and Optics Facility (IOF) of IST Austria. We thank C. Gehring for suggestions
and advice; and K. U. Torii and G. Stacey for seeds and plasmids. This project was
funded by a European Research Council Advanced Grant (ETAP-742985). M.F.K. and R.N.
acknowledge the support of the EU MSCA-IF project CrysPINs (792329). M.K. was supported
by the project POWR.03.05.00-00-Z302/17 Universitas Copernicana Thoruniensis in
Futuro–IDS “Academia Copernicana”. CIDG acknowledges support from UKRI under Future
Leaders Fellowship grant number MR/T020652/1.
article_processing_charge: No
article_type: original
author:
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Mateusz
full_name: Kwiatkowski, Mateusz
last_name: Kwiatkowski
- first_name: Huihuang
full_name: Chen, Huihuang
id: 83c96512-15b2-11ec-abd3-b7eede36184f
last_name: Chen
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Charo I.
full_name: del Genio, Charo I.
last_name: del Genio
- first_name: Martin F.
full_name: Kubeš, Martin F.
last_name: Kubeš
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Krzysztof
full_name: Jaworski, Krzysztof
last_name: Jaworski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Qi L, Kwiatkowski M, Chen H, et al. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 2022;611(7934):133-138. doi:10.1038/s41586-022-05369-7
apa: Qi, L., Kwiatkowski, M., Chen, H., Hörmayer, L., Sinclair, S. A., Zou, M.,
… Friml, J. (2022). Adenylate cyclase activity of TIR1/AFB auxin receptors in
plants. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05369-7
chicago: Qi, Linlin, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hörmayer, Scott A
Sinclair, Minxia Zou, Charo I. del Genio, et al. “Adenylate Cyclase Activity of
TIR1/AFB Auxin Receptors in Plants.” Nature. Springer Nature, 2022. https://doi.org/10.1038/s41586-022-05369-7.
ieee: L. Qi et al., “Adenylate cyclase activity of TIR1/AFB auxin receptors
in plants,” Nature, vol. 611, no. 7934. Springer Nature, pp. 133–138, 2022.
ista: Qi L, Kwiatkowski M, Chen H, Hörmayer L, Sinclair SA, Zou M, del Genio CI,
Kubeš MF, Napier R, Jaworski K, Friml J. 2022. Adenylate cyclase activity of TIR1/AFB
auxin receptors in plants. Nature. 611(7934), 133–138.
mla: Qi, Linlin, et al. “Adenylate Cyclase Activity of TIR1/AFB Auxin Receptors
in Plants.” Nature, vol. 611, no. 7934, Springer Nature, 2022, pp. 133–38,
doi:10.1038/s41586-022-05369-7.
short: L. Qi, M. Kwiatkowski, H. Chen, L. Hörmayer, S.A. Sinclair, M. Zou, C.I.
del Genio, M.F. Kubeš, R. Napier, K. Jaworski, J. Friml, Nature 611 (2022) 133–138.
corr_author: '1'
date_created: 2023-01-12T12:06:05Z
date_published: 2022-11-03T00:00:00Z
date_updated: 2025-04-14T07:45:02Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41586-022-05369-7
ec_funded: 1
external_id:
isi:
- '000875061600013'
pmid:
- '36289340'
intvolume: ' 611'
isi: 1
issue: '7934'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://wrap.warwick.ac.uk/168325/1/WRAP-denylate-cyclase-activity-TIR1-AFB-auxin-receptors-root-growth-22.pdf
month: '11'
oa: 1
oa_version: Submitted Version
page: 133-138
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 611
year: '2022'
...
---
_id: '9656'
abstract:
- lang: eng
text: Tropisms, growth responses to environmental stimuli such as light or gravity,
are spectacular examples of adaptive plant development. The plant hormone auxin
serves as a major coordinative signal. The PIN auxin exporters, through their
dynamic polar subcellular localizations, redirect auxin fluxes in response to
environmental stimuli and the resulting auxin gradients across organs underly
differential cell elongation and bending. In this review, we discuss recent advances
concerning regulations of PIN polarity during tropisms, focusing on PIN phosphorylation
and trafficking. We also cover how environmental cues regulate PIN actions during
tropisms, and a crucial role of auxin feedback on PIN polarity during bending
termination. Finally, the interactions between different tropisms are reviewed
to understand plant adaptive growth in the natural environment.
acknowledgement: We are grateful to Lukas Fiedler, Alexandra Mally (IST Austria) and
Dr. Bartel Vanholme (VIB, Ghent) for their critical comments on the manuscript.
We apologize to those researchers whose great work was not cited. This work is supported
by the European Research Council under the European Union’s Horizon 2020 research
and innovation Programme (ERC grant agreement number 742985), and the Austrian Science
Fund (FWF, grant number I 3630-B25) to JF. HH is supported by the China Scholarship
Council (CSC scholarship, 201506870018) and a starting grant from Jiangxi Agriculture
University (9232308314).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: SS
full_name: Alotaibi, SS
last_name: Alotaibi
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Han H, Adamowski M, Qi L, Alotaibi S, Friml J. PIN-mediated polar auxin transport
regulations in plant tropic responses. New Phytologist. 2021;232(2):510-522.
doi:10.1111/nph.17617
apa: Han, H., Adamowski, M., Qi, L., Alotaibi, S., & Friml, J. (2021). PIN-mediated
polar auxin transport regulations in plant tropic responses. New Phytologist.
Wiley. https://doi.org/10.1111/nph.17617
chicago: Han, Huibin, Maciek Adamowski, Linlin Qi, SS Alotaibi, and Jiří Friml.
“PIN-Mediated Polar Auxin Transport Regulations in Plant Tropic Responses.” New
Phytologist. Wiley, 2021. https://doi.org/10.1111/nph.17617.
ieee: H. Han, M. Adamowski, L. Qi, S. Alotaibi, and J. Friml, “PIN-mediated polar
auxin transport regulations in plant tropic responses,” New Phytologist,
vol. 232, no. 2. Wiley, pp. 510–522, 2021.
ista: Han H, Adamowski M, Qi L, Alotaibi S, Friml J. 2021. PIN-mediated polar auxin
transport regulations in plant tropic responses. New Phytologist. 232(2), 510–522.
mla: Han, Huibin, et al. “PIN-Mediated Polar Auxin Transport Regulations in Plant
Tropic Responses.” New Phytologist, vol. 232, no. 2, Wiley, 2021, pp. 510–22,
doi:10.1111/nph.17617.
short: H. Han, M. Adamowski, L. Qi, S. Alotaibi, J. Friml, New Phytologist 232 (2021)
510–522.
corr_author: '1'
date_created: 2021-07-14T15:29:14Z
date_published: 2021-10-01T00:00:00Z
date_updated: 2025-04-14T07:45:00Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.17617
ec_funded: 1
external_id:
isi:
- '000680587100001'
pmid:
- '34254313'
file:
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checksum: 6422a6eb329b52d96279daaee0fcf189
content_type: application/pdf
creator: kschuh
date_created: 2021-10-07T13:42:47Z
date_updated: 2021-10-07T13:42:47Z
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file_name: 2021_NewPhytologist_Han.pdf
file_size: 1939800
relation: main_file
success: 1
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intvolume: ' 232'
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issue: '2'
language:
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month: '10'
oa: 1
oa_version: Published Version
page: 510-522
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN-mediated polar auxin transport regulations in plant tropic responses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 232
year: '2021'
...