[{"date_published":"2021-11-05T00:00:00Z","page":"329-343","article_type":"original","citation":{"ama":"Kashkan I, Hrtyan M, Retzer K, et al. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. 2021;233:329-343. doi:10.1111/nph.17792","ista":"Kashkan I, Hrtyan M, Retzer K, Humpolíčková J, Jayasree A, Filepová R, Vondráková Z, Simon S, Rombaut D, Jacobs TB, Frilander MJ, Hejátko J, Friml J, Petrášek J, Růžička K. 2021. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. 233, 329–343.","apa":"Kashkan, I., Hrtyan, M., Retzer, K., Humpolíčková, J., Jayasree, A., Filepová, R., … Růžička, K. (2021). Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. Wiley. https://doi.org/10.1111/nph.17792","ieee":"I. Kashkan et al., “Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana,” New Phytologist, vol. 233. Wiley, pp. 329–343, 2021.","mla":"Kashkan, Ivan, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” New Phytologist, vol. 233, Wiley, 2021, pp. 329–43, doi:10.1111/nph.17792.","short":"I. Kashkan, M. Hrtyan, K. Retzer, J. Humpolíčková, A. Jayasree, R. Filepová, Z. Vondráková, S. Simon, D. Rombaut, T.B. Jacobs, M.J. Frilander, J. Hejátko, J. Friml, J. Petrášek, K. Růžička, New Phytologist 233 (2021) 329–343.","chicago":"Kashkan, Ivan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy Jayasree, Roberta Filepová, Zuzana Vondráková, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” New Phytologist. Wiley, 2021. https://doi.org/10.1111/nph.17792."},"publication":"New Phytologist","article_processing_charge":"No","day":"05","scopus_import":"1","oa_version":"Preprint","intvolume":" 233","status":"public","title":"Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana","_id":"10282","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","abstract":[{"text":"Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.","lang":"eng"}],"type":"journal_article","language":[{"iso":"eng"}],"doi":"10.1111/nph.17792","quality_controlled":"1","isi":1,"oa":1,"main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/2020.05.02.074070v2","open_access":"1"}],"external_id":{"pmid":["34637542"],"isi":["000714678100001"]},"publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"month":"11","volume":233,"date_updated":"2023-08-14T11:46:43Z","date_created":"2021-11-14T23:01:24Z","author":[{"full_name":"Kashkan, Ivan","last_name":"Kashkan","first_name":"Ivan"},{"full_name":"Hrtyan, Mónika","id":"45A71A74-F248-11E8-B48F-1D18A9856A87","last_name":"Hrtyan","first_name":"Mónika"},{"full_name":"Retzer, Katarzyna","last_name":"Retzer","first_name":"Katarzyna"},{"last_name":"Humpolíčková","first_name":"Jana","full_name":"Humpolíčková, Jana"},{"full_name":"Jayasree, Aswathy","first_name":"Aswathy","last_name":"Jayasree"},{"full_name":"Filepová, Roberta","last_name":"Filepová","first_name":"Roberta"},{"first_name":"Zuzana","last_name":"Vondráková","full_name":"Vondráková, Zuzana"},{"orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu","full_name":"Simon, Sibu"},{"first_name":"Debbie","last_name":"Rombaut","full_name":"Rombaut, Debbie"},{"last_name":"Jacobs","first_name":"Thomas B.","full_name":"Jacobs, Thomas B."},{"first_name":"Mikko J.","last_name":"Frilander","full_name":"Frilander, Mikko J."},{"full_name":"Hejátko, Jan","first_name":"Jan","last_name":"Hejátko"},{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"full_name":"Růžička, Kamil","first_name":"Kamil","last_name":"Růžička"}],"department":[{"_id":"JiFr"}],"publisher":"Wiley","publication_status":"published","pmid":1,"acknowledgement":"We thank Claus Schwechheimer for the pin34 and pin347 seeds, Yuliia Mironova for technical assistance, Ksenia Timofeyenko and Dmitry Konovalov for help with the evolutional analysis, Konstantin Kutashev and Siarhei Dabravolski for assistance with FRET-FLIM, Huibin Han for advice with hypocotyl imaging, Karel Müller for the initial qRT-PCR on the tobacco cell lines, Stano Pekár for suggestions regarding the statistical analysis of the morphodynamic measurements, and Jozef Mravec, Dolf Weijers and Lindy Abas for their comments on the manuscript. This work was supported by the Czech Science Foundation (projects 16-26428S and 19-23773S to IK, MH and KRůžička, 19-18917S to JHumpolíčková and 18-26981S to JF), and the Ministry of Education, Youth and Sports of the Czech Republic (MEYS, CZ.02.1.01/0.0/0.0/16_019/0000738) to KRůžička and JHejátko. The imaging facilities of the Institute of Experimental Botany and CEITEC are supported by MEYS (LM2018129 – Czech BioImaging and CZ.02.1.01/0.0/0.0/16_013/0001775). The authors declare no competing interests.","year":"2021"},{"citation":{"short":"T.R. Moturu, S. Sinha, H. Salava, S. Thula, T. Nodzyński, R.S. Vařeková, J. Friml, S. Simon, Plants 9 (2020).","mla":"Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of Proteins Involved in MiRNA Maturation Pathway.” Plants, vol. 9, no. 3, 299, MDPI, 2020, doi:10.3390/plants9030299.","chicago":"Moturu, Taraka Ramji, Sansrity Sinha, Hymavathi Salava, Sravankumar Thula, Tomasz Nodzyński, Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular Evolution and Diversification of Proteins Involved in MiRNA Maturation Pathway.” Plants. MDPI, 2020. https://doi.org/10.3390/plants9030299.","ama":"Moturu TR, Sinha S, Salava H, et al. Molecular evolution and diversification of proteins involved in miRNA maturation pathway. Plants. 2020;9(3). doi:10.3390/plants9030299","ieee":"T. R. Moturu et al., “Molecular evolution and diversification of proteins involved in miRNA maturation pathway,” Plants, vol. 9, no. 3. MDPI, 2020.","apa":"Moturu, T. R., Sinha, S., Salava, H., Thula, S., Nodzyński, T., Vařeková, R. S., … Simon, S. (2020). Molecular evolution and diversification of proteins involved in miRNA maturation pathway. Plants. MDPI. https://doi.org/10.3390/plants9030299","ista":"Moturu TR, Sinha S, Salava H, Thula S, Nodzyński T, Vařeková RS, Friml J, Simon S. 2020. Molecular evolution and diversification of proteins involved in miRNA maturation pathway. Plants. 9(3), 299."},"publication":"Plants","article_type":"original","date_published":"2020-03-01T00:00:00Z","scopus_import":"1","has_accepted_license":"1","article_processing_charge":"No","day":"01","_id":"7582","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":" 9","ddc":["580"],"title":"Molecular evolution and diversification of proteins involved in miRNA maturation pathway","status":"public","oa_version":"Published Version","file":[{"file_name":"2020_Plants_Moturu.pdf","access_level":"open_access","creator":"dernst","file_size":2373484,"content_type":"application/pdf","file_id":"7614","relation":"main_file","date_created":"2020-03-23T13:37:00Z","date_updated":"2020-07-14T12:48:00Z","checksum":"6d5af3e17266a48996b4af4e67e88a85"}],"type":"journal_article","issue":"3","abstract":[{"lang":"eng","text":"Small RNAs (smRNA, 19–25 nucleotides long), which are transcribed by RNA polymerase II, regulate the expression of genes involved in a multitude of processes in eukaryotes. miRNA biogenesis and the proteins involved in the biogenesis pathway differ across plant and animal lineages. The major proteins constituting the biogenesis pathway, namely, the Dicers (DCL/DCR) and Argonautes (AGOs), have been extensively studied. However, the accessory proteins (DAWDLE (DDL), SERRATE (SE), and TOUGH (TGH)) of the pathway that differs across the two lineages remain largely uncharacterized. We present the first detailed report on the molecular evolution and divergence of these proteins across eukaryotes. Although DDL is present in eukaryotes and prokaryotes, SE and TGH appear to be specific to eukaryotes. The addition/deletion of specific domains and/or domain-specific sequence divergence in the three proteins points to the observed functional divergence of these proteins across the two lineages, which correlates with the differences in miRNA length across the two lineages. Our data enhance the current understanding of the structure–function relationship of these proteins and reveals previous unexplored crucial residues in the three proteins that can be used as a basis for further functional characterization. The data presented here on the number of miRNAs in crown eukaryotic lineages are consistent with the notion of the expansion of the number of miRNA-coding genes in animal and plant lineages correlating with organismal complexity. Whether this difference in functionally correlates with the diversification (or presence/absence) of the three proteins studied here or the miRNA signaling in the plant and animal lineages is unclear. Based on our results of the three proteins studied here and previously available data concerning the evolution of miRNA genes in the plant and animal lineages, we believe that miRNAs probably evolved once in the ancestor to crown eukaryotes and have diversified independently in the eukaryotes."}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"pmid":["32121542"],"isi":["000525315000035"]},"project":[{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"}],"isi":1,"quality_controlled":"1","doi":"10.3390/plants9030299","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["22237747"]},"month":"03","pmid":1,"year":"2020","publisher":"MDPI","department":[{"_id":"JiFr"}],"publication_status":"published","author":[{"full_name":"Moturu, Taraka Ramji","first_name":"Taraka Ramji","last_name":"Moturu"},{"full_name":"Sinha, Sansrity","last_name":"Sinha","first_name":"Sansrity"},{"last_name":"Salava","first_name":"Hymavathi","full_name":"Salava, Hymavathi"},{"last_name":"Thula","first_name":"Sravankumar","full_name":"Thula, Sravankumar"},{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"full_name":"Vařeková, Radka Svobodová","first_name":"Radka Svobodová","last_name":"Vařeková"},{"full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Simon, Sibu","first_name":"Sibu","last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741"}],"volume":9,"date_created":"2020-03-15T23:00:52Z","date_updated":"2023-08-18T07:07:08Z","article_number":"299","ec_funded":1,"file_date_updated":"2020-07-14T12:48:00Z"},{"author":[{"first_name":"Ligang","last_name":"Fan","full_name":"Fan, Ligang"},{"last_name":"Zhao","first_name":"Lei","full_name":"Zhao, Lei"},{"first_name":"Wei","last_name":"Hu","full_name":"Hu, Wei"},{"first_name":"Weina","last_name":"Li","full_name":"Li, Weina"},{"full_name":"Novák, Ondřej","first_name":"Ondřej","last_name":"Novák"},{"full_name":"Strnad, Miroslav","first_name":"Miroslav","last_name":"Strnad"},{"full_name":"Simon, Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","first_name":"Sibu","last_name":"Simon"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Shen, Jinbo","last_name":"Shen","first_name":"Jinbo"},{"first_name":"Liwen","last_name":"Jiang","full_name":"Jiang, Liwen"},{"full_name":"Qiu, Quan","first_name":"Quan","last_name":"Qiu"}],"volume":41,"date_updated":"2023-09-13T09:03:18Z","date_created":"2018-12-11T11:46:36Z","pmid":1,"year":"2018","acknowledgement":"This work was supported by the National Natural Science Foundation of China (31571464, 31371438 and 31070222 to Q.S.Q.), the National Basic Research Program of China (973 project, 2013CB429904 to Q.S.Q.), the Research Fund for the Doctoral Program of Higher Education of China (20130211110001 to Q.S.Q.), the Ministry of Education, Youth and Sports of the Czech Republic (the National Program for Sustainability I, LO1204), and The Czech Science Foundation GAČR (GA13–40637S) to JF. We thank Dr. Tom J. Guilfoyle for DR5::GUS line and Dr. Jia Li for pBIB‐RFP vector and DR5::GFP line. We thank Liping Guan and Yang Zhao for their help with the confocal microscope assay. ","department":[{"_id":"JiFr"}],"publisher":"Wiley-Blackwell","publication_status":"published","publist_id":"7359","file_date_updated":"2020-07-14T12:46:32Z","doi":"10.1111/pce.13153","language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"external_id":{"pmid":["29360148"],"isi":["000426870500012"]},"isi":1,"quality_controlled":"1","month":"05","file":[{"file_id":"7042","relation":"main_file","date_created":"2019-11-18T16:22:22Z","date_updated":"2020-07-14T12:46:32Z","checksum":"6a20f843565f962cb20281cdf5e40914","file_name":"2018_PlantCellEnv_Fan.pdf","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_size":1937976}],"oa_version":"Submitted Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"462","intvolume":" 41","title":"NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development","status":"public","ddc":["580"],"abstract":[{"lang":"eng","text":"AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control auxin homeostasis and auxin-mediated development. We found that nhx5 nhx6 exhibited growth variations of auxin-related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the ER-localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were co-localized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H+-leak pathway provides a fine-tuning mechanism that controls cellular auxin fluxes. "}],"type":"journal_article","date_published":"2018-05-01T00:00:00Z","citation":{"mla":"Fan, Ligang, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum and Auxin-Mediated Development.” Plant, Cell and Environment, vol. 41, Wiley-Blackwell, 2018, pp. 850–64, doi:10.1111/pce.13153.","short":"L. Fan, L. Zhao, W. Hu, W. Li, O. Novák, M. Strnad, S. Simon, J. Friml, J. Shen, L. Jiang, Q. Qiu, Plant, Cell and Environment 41 (2018) 850–864.","chicago":"Fan, Ligang, Lei Zhao, Wei Hu, Weina Li, Ondřej Novák, Miroslav Strnad, Sibu Simon, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum and Auxin-Mediated Development.” Plant, Cell and Environment. Wiley-Blackwell, 2018. https://doi.org/10.1111/pce.13153.","ama":"Fan L, Zhao L, Hu W, et al. NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, Cell and Environment. 2018;41:850-864. doi:10.1111/pce.13153","ista":"Fan L, Zhao L, Hu W, Li W, Novák O, Strnad M, Simon S, Friml J, Shen J, Jiang L, Qiu Q. 2018. NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, Cell and Environment. 41, 850–864.","ieee":"L. Fan et al., “NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development,” Plant, Cell and Environment, vol. 41. Wiley-Blackwell, pp. 850–864, 2018.","apa":"Fan, L., Zhao, L., Hu, W., Li, W., Novák, O., Strnad, M., … Qiu, Q. (2018). NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. Plant, Cell and Environment. Wiley-Blackwell. https://doi.org/10.1111/pce.13153"},"publication":"Plant, Cell and Environment","page":"850 - 864","article_type":"original","article_processing_charge":"No","has_accepted_license":"1","day":"01","scopus_import":"1"},{"type":"journal_article","abstract":[{"text":"Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.","lang":"eng"}],"issue":"1","_id":"1417","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","title":"PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis","status":"public","ddc":["581"],"intvolume":" 211","pubrep_id":"1004","oa_version":"Submitted Version","file":[{"creator":"system","content_type":"application/pdf","file_size":3828383,"access_level":"open_access","file_name":"IST-2018-1004-v1+1_Simon_NewPhytol_2016_proof.pdf","checksum":"23522ced3508ffe7a4f247c4230e6493","date_updated":"2020-07-14T12:44:53Z","date_created":"2018-12-12T10:13:32Z","file_id":"5016","relation":"main_file"}],"scopus_import":1,"day":"01","has_accepted_license":"1","publication":"New Phytologist","citation":{"ama":"Simon S, Skůpa P, Viaene T, et al. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist. 2016;211(1):65-74. doi:10.1111/nph.14019","ista":"Simon S, Skůpa P, Viaene T, Zwiewka M, Tejos R, Klíma P, Čarná M, Rolčík J, De Rycke R, Moreno I, Dobrev P, Orellana A, Zažímalová E, Friml J. 2016. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist. 211(1), 65–74.","ieee":"S. Simon et al., “PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis,” New Phytologist, vol. 211, no. 1. Wiley-Blackwell, pp. 65–74, 2016.","apa":"Simon, S., Skůpa, P., Viaene, T., Zwiewka, M., Tejos, R., Klíma, P., … Friml, J. (2016). PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist. Wiley-Blackwell. https://doi.org/10.1111/nph.14019","mla":"Simon, Sibu, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” New Phytologist, vol. 211, no. 1, Wiley-Blackwell, 2016, pp. 65–74, doi:10.1111/nph.14019.","short":"S. Simon, P. Skůpa, T. Viaene, M. Zwiewka, R. Tejos, P. Klíma, M. Čarná, J. Rolčík, R. De Rycke, I. Moreno, P. Dobrev, A. Orellana, E. Zažímalová, J. Friml, New Phytologist 211 (2016) 65–74.","chicago":"Simon, Sibu, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos, Petr Klíma, Mária Čarná, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” New Phytologist. Wiley-Blackwell, 2016. https://doi.org/10.1111/nph.14019."},"page":"65 - 74","date_published":"2016-07-01T00:00:00Z","file_date_updated":"2020-07-14T12:44:53Z","publist_id":"5790","acknowledgement":"This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, project CEITEC (CZ.1.05/1.1.00/02.0068) and the Czech Science Foundation GACR (project no. 13-4063 7S to J.F.)","year":"2016","publication_status":"published","publisher":"Wiley-Blackwell","department":[{"_id":"JiFr"}],"author":[{"full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu"},{"full_name":"Skůpa, Petr","last_name":"Skůpa","first_name":"Petr"},{"full_name":"Viaene, Tom","first_name":"Tom","last_name":"Viaene"},{"first_name":"Marta","last_name":"Zwiewka","full_name":"Zwiewka, Marta"},{"full_name":"Tejos, Ricardo","first_name":"Ricardo","last_name":"Tejos"},{"full_name":"Klíma, Petr","first_name":"Petr","last_name":"Klíma"},{"first_name":"Mária","last_name":"Čarná","full_name":"Čarná, Mária"},{"first_name":"Jakub","last_name":"Rolčík","full_name":"Rolčík, Jakub"},{"last_name":"De Rycke","first_name":"Riet","full_name":"De Rycke, Riet"},{"full_name":"Moreno, Ignacio","last_name":"Moreno","first_name":"Ignacio"},{"full_name":"Dobrev, Petre","first_name":"Petre","last_name":"Dobrev"},{"full_name":"Orellana, Ariel","last_name":"Orellana","first_name":"Ariel"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí"}],"date_created":"2018-12-11T11:51:54Z","date_updated":"2021-01-12T06:50:36Z","volume":211,"month":"07","oa":1,"quality_controlled":"1","doi":"10.1111/nph.14019","language":[{"iso":"eng"}]},{"doi":"10.1016/j.cub.2015.01.015","date_published":"2015-02-12T00:00:00Z","language":[{"iso":"eng"}],"citation":{"ista":"Sasse J, Simon S, Gübeli C, Liu G, Cheng X, Friml J, Bouwmeester H, Martinoia E, Borghi L. 2015. Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional strigolactone transport. Current Biology. 25(5), 647–655.","ieee":"J. Sasse et al., “Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional strigolactone transport,” Current Biology, vol. 25, no. 5. Cell Press, pp. 647–655, 2015.","apa":"Sasse, J., Simon, S., Gübeli, C., Liu, G., Cheng, X., Friml, J., … Borghi, L. (2015). Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional strigolactone transport. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2015.01.015","ama":"Sasse J, Simon S, Gübeli C, et al. Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional strigolactone transport. Current Biology. 2015;25(5):647-655. doi:10.1016/j.cub.2015.01.015","chicago":"Sasse, Joëlle, Sibu Simon, Christian Gübeli, Guowei Liu, Xi Cheng, Jiří Friml, Harro Bouwmeester, Enrico Martinoia, and Lorenzo Borghi. “Asymmetric Localizations of the ABC Transporter PaPDR1 Trace Paths of Directional Strigolactone Transport.” Current Biology. Cell Press, 2015. https://doi.org/10.1016/j.cub.2015.01.015.","mla":"Sasse, Joëlle, et al. “Asymmetric Localizations of the ABC Transporter PaPDR1 Trace Paths of Directional Strigolactone Transport.” Current Biology, vol. 25, no. 5, Cell Press, 2015, pp. 647–55, doi:10.1016/j.cub.2015.01.015.","short":"J. Sasse, S. Simon, C. Gübeli, G. Liu, X. Cheng, J. Friml, H. Bouwmeester, E. Martinoia, L. Borghi, Current Biology 25 (2015) 647–655."},"publication":"Current Biology","page":"647 - 655","quality_controlled":"1","month":"02","day":"12","scopus_import":1,"author":[{"full_name":"Sasse, Joëlle","first_name":"Joëlle","last_name":"Sasse"},{"first_name":"Sibu","last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu"},{"full_name":"Gübeli, Christian","last_name":"Gübeli","first_name":"Christian"},{"last_name":"Liu","first_name":"Guowei","full_name":"Liu, Guowei"},{"full_name":"Cheng, Xi","first_name":"Xi","last_name":"Cheng"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"},{"full_name":"Bouwmeester, Harro","last_name":"Bouwmeester","first_name":"Harro"},{"first_name":"Enrico","last_name":"Martinoia","full_name":"Martinoia, Enrico"},{"full_name":"Borghi, Lorenzo","first_name":"Lorenzo","last_name":"Borghi"}],"oa_version":"None","volume":25,"date_created":"2018-12-11T11:52:35Z","date_updated":"2021-01-12T06:51:27Z","_id":"1536","acknowledgement":"This work was funded by a grant of the Swiss National Foundation to E.M.\r\nWe thank Dr. José María Mateos (University of Zurich) for providing us with the vibratome, Prof. Dolf Weijers (Wageningen University, the Netherlands) for shipping us his set of ligation-independent cloning vectors, Prof. Bruno Humbel (University of Lausanne) for suggestions on GFP-PDR1 detection, and Dr. Undine Krügel (University of Zurich) and Prof. Michal Jasinski (Polish Academy of Science) for hints on protein quantification.","year":"2015","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":" 25","department":[{"_id":"JiFr"}],"publisher":"Cell Press","publication_status":"published","status":"public","title":"Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional strigolactone transport","publist_id":"5635","issue":"5","abstract":[{"lang":"eng","text":"Strigolactones, first discovered as germination stimulants for parasitic weeds [1], are carotenoid-derived phytohormones that play major roles in inhibiting lateral bud outgrowth and promoting plant-mycorrhizal symbiosis [2-4]. Furthermore, strigolactones are involved in the regulation of lateral and adventitious root development, root cell division [5, 6], secondary growth [7], and leaf senescence [8]. Recently, we discovered the strigolactone transporter Petunia axillaris PLEIOTROPIC DRUG RESISTANCE 1 (PaPDR1), which is required for efficient mycorrhizal colonization and inhibition of lateral bud outgrowth [9]. However, how strigolactones are transported through the plant remained unknown. Here we show that PaPDR1 exhibits a cell-type-specific asymmetric localization in different root tissues. In root tips, PaPDR1 is co-expressed with the strigolactone biosynthetic gene DAD1 (CCD8), and it is localized at the apical membrane of root hypodermal cells, presumably mediating the shootward transport of strigolactone. Above the root tip, in the hypodermal passage cells that form gates for the entry of mycorrhizal fungi, PaPDR1 is present in the outer-lateral membrane, compatible with its postulated function as strigolactone exporter from root to soil. Transport studies are in line with our localization studies since (1) a papdr1 mutant displays impaired transport of strigolactones out of the root tip to the shoot as well as into the rhizosphere and (2) DAD1 expression and PIN1/PIN2 levels change in plants deregulated for PDR1 expression, suggestive of variations in endogenous strigolactone contents. In conclusion, our results indicate that the polar localizations of PaPDR1 mediate directional shootward strigolactone transport as well as localized exudation into the soil."}],"type":"journal_article"},{"publication_status":"published","department":[{"_id":"JiFr"},{"_id":"EM-Fac"}],"publisher":"Oxford University Press","acknowledgement":"This work was supported by ERC Independent Research grant (ERC-2011-StG- 20101109-PSDP to JF); the European Social Fund and the state budget of the Czech Republic [the project ‘Employment of Newly Graduated Doctors of Science for Scientific Excellence’ (CZ.1.07/2.3.00/30.0009) to TN]; the Czech Science Foundation (GACR) [project 13-40637S to JF].","year":"2015","date_created":"2018-12-11T11:52:44Z","date_updated":"2023-02-23T10:04:26Z","volume":66,"author":[{"full_name":"Grones, Peter","last_name":"Grones","first_name":"Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Chen, Xu","first_name":"Xu","last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu","full_name":"Simon, Sibu"},{"id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315","first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter"},{"last_name":"De Rycke","first_name":"Riet","full_name":"De Rycke, Riet"},{"full_name":"Nodzyński, Tomasz","first_name":"Tomasz","last_name":"Nodzyński"},{"last_name":"Zažímalová","first_name":"Eva","full_name":"Zažímalová, Eva"},{"last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"}],"publist_id":"5609","ec_funded":1,"quality_controlled":"1","project":[{"name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"language":[{"iso":"eng"}],"doi":"10.1093/jxb/erv177","month":"08","status":"public","title":"Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles","intvolume":" 66","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"1562","oa_version":"None","type":"journal_article","abstract":[{"lang":"eng","text":"The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxin-binding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor."}],"issue":"16","article_type":"original","page":"5055 - 5065","publication":"Journal of Experimental Botany","citation":{"ama":"Grones P, Chen X, Simon S, et al. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. 2015;66(16):5055-5065. doi:10.1093/jxb/erv177","ista":"Grones P, Chen X, Simon S, Kaufmann W, De Rycke R, Nodzyński T, Zažímalová E, Friml J. 2015. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. 66(16), 5055–5065.","ieee":"P. Grones et al., “Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles,” Journal of Experimental Botany, vol. 66, no. 16. Oxford University Press, pp. 5055–5065, 2015.","apa":"Grones, P., Chen, X., Simon, S., Kaufmann, W., De Rycke, R., Nodzyński, T., … Friml, J. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/erv177","mla":"Grones, Peter, et al. “Auxin-Binding Pocket of ABP1 Is Crucial for Its Gain-of-Function Cellular and Developmental Roles.” Journal of Experimental Botany, vol. 66, no. 16, Oxford University Press, 2015, pp. 5055–65, doi:10.1093/jxb/erv177.","short":"P. Grones, X. Chen, S. Simon, W. Kaufmann, R. De Rycke, T. Nodzyński, E. Zažímalová, J. Friml, Journal of Experimental Botany 66 (2015) 5055–5065.","chicago":"Grones, Peter, Xu Chen, Sibu Simon, Walter Kaufmann, Riet De Rycke, Tomasz Nodzyński, Eva Zažímalová, and Jiří Friml. “Auxin-Binding Pocket of ABP1 Is Crucial for Its Gain-of-Function Cellular and Developmental Roles.” Journal of Experimental Botany. Oxford University Press, 2015. https://doi.org/10.1093/jxb/erv177."},"date_published":"2015-08-01T00:00:00Z","scopus_import":1,"day":"01"},{"language":[{"iso":"eng"}],"doi":"10.1007/978-1-62703-592-7_23","quality_controlled":"1","month":"01","publication_identifier":{"issn":["10643745"]},"date_created":"2018-12-11T11:56:32Z","date_updated":"2021-01-12T06:56:15Z","volume":1056,"author":[{"full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu"},{"first_name":"Petr","last_name":"Skůpa","full_name":"Skůpa, Petr"},{"full_name":"Dobrev, Petre","last_name":"Dobrev","first_name":"Petre"},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"publication_status":"published","department":[{"_id":"JiFr"}],"editor":[{"full_name":"Hicks, Glenn","last_name":"Hicks","first_name":"Glenn"},{"first_name":"Stéphanie","last_name":"Robert","full_name":"Robert, Stéphanie"}],"publisher":"Springer","year":"2014","publist_id":"4704","date_published":"2014-01-01T00:00:00Z","page":"255 - 264","publication":"Plant Chemical Genomics","citation":{"short":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:, G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.","mla":"Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” Plant Chemical Genomics, edited by Glenn Hicks and Stéphanie Robert, vol. 1056, Springer, 2014, pp. 255–64, doi:10.1007/978-1-62703-592-7_23.","chicago":"Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” In Plant Chemical Genomics, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64. Methods in Molecular Biology. Springer, 2014. https://doi.org/10.1007/978-1-62703-592-7_23.","ama":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds. Plant Chemical Genomics. Vol 1056. Methods in Molecular Biology. Springer; 2014:255-264. doi:10.1007/978-1-62703-592-7_23","apa":"Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., & Friml, J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In G. Hicks & S. Robert (Eds.), Plant Chemical Genomics (Vol. 1056, pp. 255–264). Springer. https://doi.org/10.1007/978-1-62703-592-7_23","ieee":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml, “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters,” in Plant Chemical Genomics, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014, pp. 255–264.","ista":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Plant Chemical Genomics. Methods in Molecular Biology, vol. 1056, 255–264."},"day":"01","series_title":"Methods in Molecular Biology","scopus_import":1,"oa_version":"None","title":"Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters","status":"public","intvolume":" 1056","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","_id":"2245","abstract":[{"lang":"eng","text":"Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. "}],"alternative_title":["Methods in Molecular Biology"],"type":"book_chapter"},{"month":"12","oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.12437"}],"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"}],"quality_controlled":"1","doi":"10.1111/nph.12437","language":[{"iso":"eng"}],"publist_id":"4460","ec_funded":1,"year":"2013","acknowledgement":"The authors thank Dr Christian Luschnig (University of Natural Resources and Life Sciences (BOKU), Vienna, Austria) for the anti-PIN2 antibody, Professor Mark Estelle (University of California, San Diego, CA, USA) for tir1-1 mutant seeds and, last but not least, to Dr David Morris for critical reading of the manuscript. We also thank Markéta Pařezová and Jana Stýblová for excellent technical assistance. This work was supported by the Grant Agency of the Czech Republic (P305/11/0797 to E.Z. and 13-40637S to J.F.), the Central European Institute of Technology project CZ.1.05/1.1.00/02.0068 from the European Regional Development Fund and by a European Research Council starting independent research grant ERC-2011-StG-20101109-PSDP (to J.F.).","publisher":"Wiley","department":[{"_id":"JiFr"}],"publication_status":"published","author":[{"full_name":"Simon, Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","first_name":"Sibu","last_name":"Simon"},{"first_name":"Martin","last_name":"Kubeš","full_name":"Kubeš, Martin"},{"full_name":"Baster, Pawel","id":"3028BD74-F248-11E8-B48F-1D18A9856A87","first_name":"Pawel","last_name":"Baster"},{"last_name":"Robert","first_name":"Stéphanie","full_name":"Robert, Stéphanie"},{"first_name":"Petre","last_name":"Dobrev","full_name":"Dobrev, Petre"},{"last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"}],"volume":200,"date_updated":"2022-06-07T08:57:52Z","date_created":"2018-12-11T11:57:41Z","scopus_import":"1","article_processing_charge":"No","day":"01","citation":{"ama":"Simon S, Kubeš M, Baster P, et al. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. 2013;200(4):1034-1048. doi:10.1111/nph.12437","ista":"Simon S, Kubeš M, Baster P, Robert S, Dobrev P, Friml J, Petrášek J, Zažímalová E. 2013. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. 200(4), 1034–1048.","ieee":"S. Simon et al., “Defining the selectivity of processes along the auxin response chain: A study using auxin analogues,” New Phytologist, vol. 200, no. 4. Wiley, pp. 1034–1048, 2013.","apa":"Simon, S., Kubeš, M., Baster, P., Robert, S., Dobrev, P., Friml, J., … Zažímalová, E. (2013). Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. Wiley. https://doi.org/10.1111/nph.12437","mla":"Simon, Sibu, et al. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” New Phytologist, vol. 200, no. 4, Wiley, 2013, pp. 1034–48, doi:10.1111/nph.12437.","short":"S. Simon, M. Kubeš, P. Baster, S. Robert, P. Dobrev, J. Friml, J. Petrášek, E. Zažímalová, New Phytologist 200 (2013) 1034–1048.","chicago":"Simon, Sibu, Martin Kubeš, Pawel Baster, Stéphanie Robert, Petre Dobrev, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” New Phytologist. Wiley, 2013. https://doi.org/10.1111/nph.12437."},"publication":"New Phytologist","page":"1034 - 1048","article_type":"original","date_published":"2013-12-01T00:00:00Z","type":"journal_article","issue":"4","abstract":[{"lang":"eng","text":"The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery."}],"_id":"2443","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":" 200","status":"public","title":"Defining the selectivity of processes along the auxin response chain: A study using auxin analogues","oa_version":"Published Version"},{"month":"07","doi":"10.1371/journal.pone.0070069","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7"},{"_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"}],"file_date_updated":"2020-07-14T12:45:41Z","ec_funded":1,"publist_id":"4431","article_number":"e70069","author":[{"last_name":"Cazzonelli","first_name":"Christopher","full_name":"Cazzonelli, Christopher"},{"full_name":"Vanstraelen, Marleen","first_name":"Marleen","last_name":"Vanstraelen"},{"full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu"},{"last_name":"Yin","first_name":"Kuide","full_name":"Yin, Kuide"},{"full_name":"Carron Arthur, Ashley","first_name":"Ashley","last_name":"Carron Arthur"},{"last_name":"Nisar","first_name":"Nazia","full_name":"Nisar, Nazia"},{"last_name":"Tarle","first_name":"Gauri","full_name":"Tarle, Gauri"},{"full_name":"Cuttriss, Abby","first_name":"Abby","last_name":"Cuttriss"},{"full_name":"Searle, Iain","last_name":"Searle","first_name":"Iain"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva"},{"full_name":"Mathesius, Ulrike","first_name":"Ulrike","last_name":"Mathesius"},{"full_name":"Masle, Josette","first_name":"Josette","last_name":"Masle"},{"first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"},{"first_name":"Barry","last_name":"Pogson","full_name":"Pogson, Barry"}],"date_updated":"2021-01-12T06:57:41Z","date_created":"2018-12-11T11:57:52Z","volume":8,"year":"2013","publication_status":"published","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"publisher":"Public Library of Science","day":"29","has_accepted_license":"1","scopus_import":1,"date_published":"2013-07-29T00:00:00Z","publication":"PLoS One","citation":{"ama":"Cazzonelli C, Vanstraelen M, Simon S, et al. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. 2013;8(7). doi:10.1371/journal.pone.0070069","apa":"Cazzonelli, C., Vanstraelen, M., Simon, S., Yin, K., Carron Arthur, A., Nisar, N., … Pogson, B. (2013). Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0070069","ieee":"C. Cazzonelli et al., “Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development,” PLoS One, vol. 8, no. 7. Public Library of Science, 2013.","ista":"Cazzonelli C, Vanstraelen M, Simon S, Yin K, Carron Arthur A, Nisar N, Tarle G, Cuttriss A, Searle I, Benková E, Mathesius U, Masle J, Friml J, Pogson B. 2013. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. 8(7), e70069.","short":"C. Cazzonelli, M. Vanstraelen, S. Simon, K. Yin, A. Carron Arthur, N. Nisar, G. Tarle, A. Cuttriss, I. Searle, E. Benková, U. Mathesius, J. Masle, J. Friml, B. Pogson, PLoS One 8 (2013).","mla":"Cazzonelli, Christopher, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” PLoS One, vol. 8, no. 7, e70069, Public Library of Science, 2013, doi:10.1371/journal.pone.0070069.","chicago":"Cazzonelli, Christopher, Marleen Vanstraelen, Sibu Simon, Kuide Yin, Ashley Carron Arthur, Nazia Nisar, Gauri Tarle, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” PLoS One. Public Library of Science, 2013. https://doi.org/10.1371/journal.pone.0070069."},"abstract":[{"text":"Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.","lang":"eng"}],"issue":"7","type":"journal_article","pubrep_id":"393","oa_version":"Published Version","file":[{"checksum":"3be71828b6c2ba9c90eb7056e3f7f57a","date_created":"2018-12-12T10:16:34Z","date_updated":"2020-07-14T12:45:41Z","file_id":"5222","relation":"main_file","creator":"system","file_size":9003465,"content_type":"application/pdf","access_level":"open_access","file_name":"IST-2015-393-v1+1_journal.pone.0070069.pdf"}],"_id":"2472","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["580","570"],"title":"Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development","status":"public","intvolume":" 8"},{"month":"08","language":[{"iso":"eng"}],"doi":"10.1105/tpc.113.114058","quality_controlled":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784593/","open_access":"1"}],"external_id":{"pmid":["23975899"]},"oa":1,"publist_id":"7311","volume":25,"date_created":"2018-12-11T11:46:52Z","date_updated":"2021-01-12T08:01:13Z","author":[{"full_name":"Di Rubbo, Simone","last_name":"Di Rubbo","first_name":"Simone"},{"last_name":"Irani","first_name":"Niloufer","full_name":"Irani, Niloufer"},{"full_name":"Kim, Soo","last_name":"Kim","first_name":"Soo"},{"last_name":"Xu","first_name":"Zheng","full_name":"Xu, Zheng"},{"first_name":"Astrid","last_name":"Gadeyne","full_name":"Gadeyne, Astrid"},{"full_name":"Dejonghe, Wim","last_name":"Dejonghe","first_name":"Wim"},{"full_name":"Vanhoutte, Isabelle","last_name":"Vanhoutte","first_name":"Isabelle"},{"last_name":"Persiau","first_name":"Geert","full_name":"Persiau, Geert"},{"first_name":"Dominique","last_name":"Eeckhout","full_name":"Eeckhout, Dominique"},{"last_name":"Simon","first_name":"Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","full_name":"Simon, Sibu"},{"full_name":"Song, Kyungyoung","first_name":"Kyungyoung","last_name":"Song"},{"full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"De Jaeger","first_name":"Geert","full_name":"De Jaeger, Geert"},{"full_name":"Van Damme, Daniël","last_name":"Van Damme","first_name":"Daniël"},{"first_name":"Inhwan","last_name":"Hwang","full_name":"Hwang, Inhwan"},{"last_name":"Russinova","first_name":"Eugenia","full_name":"Russinova, Eugenia"}],"department":[{"_id":"JiFr"}],"publisher":"American Society of Plant Biologists","publication_status":"published","pmid":1,"year":"2013","day":"01","scopus_import":1,"date_published":"2013-08-01T00:00:00Z","page":"2986 - 2997","citation":{"chicago":"Di Rubbo, Simone, Niloufer Irani, Soo Kim, Zheng Xu, Astrid Gadeyne, Wim Dejonghe, Isabelle Vanhoutte, et al. “The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of Brassinosteroid INSENSITIVE1 in Arabidopsis.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114058.","mla":"Di Rubbo, Simone, et al. “The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of Brassinosteroid INSENSITIVE1 in Arabidopsis.” Plant Cell, vol. 25, no. 8, American Society of Plant Biologists, 2013, pp. 2986–97, doi:10.1105/tpc.113.114058.","short":"S. Di Rubbo, N. Irani, S. Kim, Z. Xu, A. Gadeyne, W. Dejonghe, I. Vanhoutte, G. Persiau, D. Eeckhout, S. Simon, K. Song, J. Kleine Vehn, J. Friml, G. De Jaeger, D. Van Damme, I. Hwang, E. Russinova, Plant Cell 25 (2013) 2986–2997.","ista":"Di Rubbo S, Irani N, Kim S, Xu Z, Gadeyne A, Dejonghe W, Vanhoutte I, Persiau G, Eeckhout D, Simon S, Song K, Kleine Vehn J, Friml J, De Jaeger G, Van Damme D, Hwang I, Russinova E. 2013. The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. 25(8), 2986–2997.","ieee":"S. Di Rubbo et al., “The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis,” Plant Cell, vol. 25, no. 8. American Society of Plant Biologists, pp. 2986–2997, 2013.","apa":"Di Rubbo, S., Irani, N., Kim, S., Xu, Z., Gadeyne, A., Dejonghe, W., … Russinova, E. (2013). The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.114058","ama":"Di Rubbo S, Irani N, Kim S, et al. The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. 2013;25(8):2986-2997. doi:10.1105/tpc.113.114058"},"publication":"Plant Cell","issue":"8","abstract":[{"text":"Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptormediated endocytosis. ","lang":"eng"}],"type":"journal_article","oa_version":"Submitted Version","intvolume":" 25","status":"public","title":"The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"509"},{"oa":1,"main_file_link":[{"url":"www.doi.org/10.1105/tpc.113.114421","open_access":"1"}],"external_id":{"pmid":["24163311"]},"quality_controlled":"1","doi":"10.1105/tpc.113.114421","language":[{"iso":"eng"}],"month":"10","pmid":1,"year":"2013","publisher":"American Society of Plant Biologists","department":[{"_id":"JiFr"}],"publication_status":"published","author":[{"full_name":"Pěnčík, Aleš","first_name":"Aleš","last_name":"Pěnčík"},{"first_name":"Biljana","last_name":"Simonovik","full_name":"Simonovik, Biljana"},{"first_name":"Sara","last_name":"Petersson","full_name":"Petersson, Sara"},{"last_name":"Henyková","first_name":"Eva","full_name":"Henyková, Eva"},{"full_name":"Simon, Sibu","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","first_name":"Sibu","last_name":"Simon"},{"full_name":"Greenham, Kathleen","last_name":"Greenham","first_name":"Kathleen"},{"full_name":"Zhang, Yi","last_name":"Zhang","first_name":"Yi"},{"first_name":"Mariusz","last_name":"Kowalczyk","full_name":"Kowalczyk, Mariusz"},{"full_name":"Estelle, Mark","first_name":"Mark","last_name":"Estelle"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"},{"full_name":"Novák, Ondřej","last_name":"Novák","first_name":"Ondřej"},{"first_name":"Göran","last_name":"Sandberg","full_name":"Sandberg, Göran"},{"full_name":"Ljung, Karin","last_name":"Ljung","first_name":"Karin"}],"volume":25,"date_updated":"2021-01-12T08:01:15Z","date_created":"2018-12-11T11:46:53Z","publist_id":"7309","citation":{"chicago":"Pěnčík, Aleš, Biljana Simonovik, Sara Petersson, Eva Henyková, Sibu Simon, Kathleen Greenham, Yi Zhang, et al. “Regulation of Auxin Homeostasis and Gradients in Arabidopsis Roots through the Formation of the Indole-3-Acetic Acid Catabolite 2-Oxindole-3-Acetic Acid.” Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114421.","short":"A. Pěnčík, B. Simonovik, S. Petersson, E. Henyková, S. Simon, K. Greenham, Y. Zhang, M. Kowalczyk, M. Estelle, E. Zažímalová, O. Novák, G. Sandberg, K. Ljung, Plant Cell 25 (2013) 3858–3870.","mla":"Pěnčík, Aleš, et al. “Regulation of Auxin Homeostasis and Gradients in Arabidopsis Roots through the Formation of the Indole-3-Acetic Acid Catabolite 2-Oxindole-3-Acetic Acid.” Plant Cell, vol. 25, no. 10, American Society of Plant Biologists, 2013, pp. 3858–70, doi:10.1105/tpc.113.114421.","ieee":"A. Pěnčík et al., “Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid,” Plant Cell, vol. 25, no. 10. American Society of Plant Biologists, pp. 3858–3870, 2013.","apa":"Pěnčík, A., Simonovik, B., Petersson, S., Henyková, E., Simon, S., Greenham, K., … Ljung, K. (2013). Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.114421","ista":"Pěnčík A, Simonovik B, Petersson S, Henyková E, Simon S, Greenham K, Zhang Y, Kowalczyk M, Estelle M, Zažímalová E, Novák O, Sandberg G, Ljung K. 2013. Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. 25(10), 3858–3870.","ama":"Pěnčík A, Simonovik B, Petersson S, et al. Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. 2013;25(10):3858-3870. doi:10.1105/tpc.113.114421"},"publication":"Plant Cell","page":"3858 - 3870","date_published":"2013-10-01T00:00:00Z","scopus_import":1,"day":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"511","intvolume":" 25","title":"Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid","status":"public","oa_version":"Published Version","type":"journal_article","issue":"10","abstract":[{"lang":"eng","text":"The native auxin, indole-3-acetic acid (IAA), is a major regulator of plant growth and development. Its nonuniform distribution between cells and tissues underlies the spatiotemporal coordination of many developmental events and responses to environmental stimuli. The regulation of auxin gradients and the formation of auxin maxima/minima most likely involve the regulation of both metabolic and transport processes. In this article, we have demonstrated that 2-oxindole-3-acetic acid (oxIAA) is a major primary IAA catabolite formed in Arabidopsis thaliana root tissues. OxIAA had little biological activity and was formed rapidly and irreversibly in response to increases in auxin levels. We further showed that there is cell type-specific regulation of oxIAA levels in the Arabidopsis root apex. We propose that oxIAA is an important element in the regulation of output from auxin gradients and, therefore, in the regulation of auxin homeostasis and response mechanisms."}]},{"type":"journal_article","extern":1,"abstract":[{"text":"Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.","lang":"eng"}],"publist_id":"3585","issue":"AN 941","publication_status":"published","status":"public","title":"ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis","intvolume":" 3","publisher":"Nature Publishing Group","year":"2012","_id":"3114","date_updated":"2021-01-12T07:41:09Z","date_created":"2018-12-11T12:01:28Z","volume":3,"author":[{"full_name":"Ding, Zhaojun","last_name":"Ding","first_name":"Zhaojun"},{"first_name":"Bangjun","last_name":"Wang","full_name":"Wang, Bangjun"},{"last_name":"Moreno","first_name":"Ignacio","full_name":"Moreno, Ignacio"},{"full_name":"Dupláková, Nikoleta","last_name":"Dupláková","first_name":"Nikoleta"},{"first_name":"Sibu","last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","full_name":"Sibu Simon"},{"full_name":"Carraro, Nicola","first_name":"Nicola","last_name":"Carraro"},{"full_name":"Reemmer, Jesica","first_name":"Jesica","last_name":"Reemmer"},{"first_name":"Aleš","last_name":"Pěnčík","full_name":"Pěnčík, Aleš"},{"last_name":"Chen","first_name":"Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","full_name":"Xu Chen"},{"full_name":"Tejos, Ricardo I","first_name":"Ricardo","last_name":"Tejos"},{"last_name":"Skůpa","first_name":"Petr","full_name":"Skůpa, Petr"},{"last_name":"Pollmann","first_name":"Stephan","full_name":"Pollmann, Stephan"},{"full_name":"Mravec, Jozef","last_name":"Mravec","first_name":"Jozef"},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"},{"full_name":"Zažímalová, Eva","first_name":"Eva","last_name":"Zažímalová"},{"first_name":"David","last_name":"Honys","full_name":"Honys, David"},{"full_name":"Rolčík, Jakub","last_name":"Rolčík","first_name":"Jakub"},{"first_name":"Angus","last_name":"Murphy","full_name":"Murphy, Angus S"},{"full_name":"Orellana, Ariel","last_name":"Orellana","first_name":"Ariel"},{"first_name":"Markus","last_name":"Geisler","full_name":"Geisler, Markus"},{"last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Jirí Friml"}],"month":"07","day":"03","quality_controlled":0,"publication":"Nature Communications","citation":{"chicago":"Ding, Zhaojun, Bangjun Wang, Ignacio Moreno, Nikoleta Dupláková, Sibu Simon, Nicola Carraro, Jesica Reemmer, et al. “ER-Localized Auxin Transporter PIN8 Regulates Auxin Homeostasis and Male Gametophyte Development in Arabidopsis.” Nature Communications. Nature Publishing Group, 2012. https://doi.org/10.1038/ncomms1941.","short":"Z. Ding, B. Wang, I. Moreno, N. Dupláková, S. Simon, N. Carraro, J. Reemmer, A. Pěnčík, X. Chen, R. Tejos, P. Skůpa, S. Pollmann, J. Mravec, J. Petrášek, E. Zažímalová, D. Honys, J. Rolčík, A. Murphy, A. Orellana, M. Geisler, J. Friml, Nature Communications 3 (2012).","mla":"Ding, Zhaojun, et al. “ER-Localized Auxin Transporter PIN8 Regulates Auxin Homeostasis and Male Gametophyte Development in Arabidopsis.” Nature Communications, vol. 3, no. AN 941, Nature Publishing Group, 2012, doi:10.1038/ncomms1941.","ieee":"Z. Ding et al., “ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis,” Nature Communications, vol. 3, no. AN 941. Nature Publishing Group, 2012.","apa":"Ding, Z., Wang, B., Moreno, I., Dupláková, N., Simon, S., Carraro, N., … Friml, J. (2012). ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms1941","ista":"Ding Z, Wang B, Moreno I, Dupláková N, Simon S, Carraro N, Reemmer J, Pěnčík A, Chen X, Tejos R, Skůpa P, Pollmann S, Mravec J, Petrášek J, Zažímalová E, Honys D, Rolčík J, Murphy A, Orellana A, Geisler M, Friml J. 2012. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. Nature Communications. 3(AN 941).","ama":"Ding Z, Wang B, Moreno I, et al. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. Nature Communications. 2012;3(AN 941). doi:10.1038/ncomms1941"},"doi":"10.1038/ncomms1941","date_published":"2012-07-03T00:00:00Z"},{"day":"01","month":"10","page":"111 - 121","quality_controlled":0,"citation":{"mla":"Robert, Stéphanie, et al. “ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis.” Cell, vol. 143, no. 1, Cell Press, 2010, pp. 111–21, doi:10.1016/j.cell.2010.09.027.","short":"S. Robert, J. Kleine Vehn, E. Barbez, M. Sauer, T. Paciorek, P. Baster, S. Vanneste, J. Zhang, S. Simon, M. Čovanová, K. Hayashi, P. Dhonukshe, Z. Yang, S. Bednarek, A. Jones, C. Luschnig, F. Aniento, E. Zažímalová, J. Friml, Cell 143 (2010) 111–121.","chicago":"Robert, Stéphanie, Jürgen Kleine Vehn, Elke Barbez, Michael Sauer, Tomasz Paciorek, Pawel Baster, Steffen Vanneste, et al. “ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis.” Cell. Cell Press, 2010. https://doi.org/10.1016/j.cell.2010.09.027.","ama":"Robert S, Kleine Vehn J, Barbez E, et al. ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell. 2010;143(1):111-121. doi:10.1016/j.cell.2010.09.027","ista":"Robert S, Kleine Vehn J, Barbez E, Sauer M, Paciorek T, Baster P, Vanneste S, Zhang J, Simon S, Čovanová M, Hayashi K, Dhonukshe P, Yang Z, Bednarek S, Jones A, Luschnig C, Aniento F, Zažímalová E, Friml J. 2010. ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell. 143(1), 111–121.","apa":"Robert, S., Kleine Vehn, J., Barbez, E., Sauer, M., Paciorek, T., Baster, P., … Friml, J. (2010). ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis. Cell. Cell Press. https://doi.org/10.1016/j.cell.2010.09.027","ieee":"S. Robert et al., “ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis,” Cell, vol. 143, no. 1. Cell Press, pp. 111–121, 2010."},"publication":"Cell","doi":"10.1016/j.cell.2010.09.027","date_published":"2010-10-01T00:00:00Z","type":"journal_article","extern":1,"issue":"1","publist_id":"3626","abstract":[{"text":"\nSpatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport.","lang":"eng"}],"intvolume":" 143","publisher":"Cell Press","status":"public","title":"ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis","publication_status":"published","year":"2010","_id":"3075","volume":143,"date_created":"2018-12-11T12:01:13Z","date_updated":"2021-01-12T07:40:52Z","author":[{"full_name":"Robert, Stéphanie","first_name":"Stéphanie","last_name":"Robert"},{"full_name":"Kleine-Vehn, Jürgen","first_name":"Jürgen","last_name":"Kleine Vehn"},{"full_name":"Barbez, Elke","last_name":"Barbez","first_name":"Elke"},{"first_name":"Michael","last_name":"Sauer","full_name":"Sauer, Michael"},{"last_name":"Paciorek","first_name":"Tomasz","full_name":"Paciorek, Tomasz"},{"full_name":"Pawel Baster","last_name":"Baster","first_name":"Pawel","id":"3028BD74-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"full_name":"Zhang, Jing","first_name":"Jing","last_name":"Zhang"},{"id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741","first_name":"Sibu","last_name":"Simon","full_name":"Sibu Simon"},{"full_name":"Čovanová, Milada","first_name":"Milada","last_name":"Čovanová"},{"full_name":"Hayashi, Kenichiro","last_name":"Hayashi","first_name":"Kenichiro"},{"first_name":"Pankaj","last_name":"Dhonukshe","full_name":"Dhonukshe, Pankaj"},{"first_name":"Zhenbiao","last_name":"Yang","full_name":"Yang, Zhenbiao"},{"full_name":"Bednarek, Sebastian Y","last_name":"Bednarek","first_name":"Sebastian"},{"first_name":"Alan","last_name":"Jones","full_name":"Jones, Alan M"},{"full_name":"Luschnig, Christian","last_name":"Luschnig","first_name":"Christian"},{"last_name":"Aniento","first_name":"Fernando","full_name":"Aniento, Fernando"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Jirí Friml"}]},{"page":"883 - 892","quality_controlled":0,"citation":{"ieee":"A. Jelínková et al., “Probing plant membranes with FM dyes: Tracking dragging or blocking?,” Plant Journal, vol. 61, no. 5. Wiley-Blackwell, pp. 883–892, 2010.","apa":"Jelínková, A., Malínská, K., Simon, S., Kleine Vehn, J., Pařezová, M., Pejchar, P., … Petrášek, J. (2010). Probing plant membranes with FM dyes: Tracking dragging or blocking? Plant Journal. Wiley-Blackwell. https://doi.org/10.1111/j.1365-313X.2009.04102.x","ista":"Jelínková A, Malínská K, Simon S, Kleine Vehn J, Pařezová M, Pejchar P, Kubeš M, Martinec J, Friml J, Zažímalová E, Petrášek J. 2010. Probing plant membranes with FM dyes: Tracking dragging or blocking? Plant Journal. 61(5), 883–892.","ama":"Jelínková A, Malínská K, Simon S, et al. Probing plant membranes with FM dyes: Tracking dragging or blocking? Plant Journal. 2010;61(5):883-892. doi:10.1111/j.1365-313X.2009.04102.x","chicago":"Jelínková, Adriana, Kateřina Malínská, Sibu Simon, Jürgen Kleine Vehn, Markéta Pařezová, Přemysl Pejchar, Martin Kubeš, et al. “Probing Plant Membranes with FM Dyes: Tracking Dragging or Blocking?” Plant Journal. Wiley-Blackwell, 2010. https://doi.org/10.1111/j.1365-313X.2009.04102.x.","short":"A. Jelínková, K. Malínská, S. Simon, J. Kleine Vehn, M. Pařezová, P. Pejchar, M. Kubeš, J. Martinec, J. Friml, E. Zažímalová, J. Petrášek, Plant Journal 61 (2010) 883–892.","mla":"Jelínková, Adriana, et al. “Probing Plant Membranes with FM Dyes: Tracking Dragging or Blocking?” Plant Journal, vol. 61, no. 5, Wiley-Blackwell, 2010, pp. 883–92, doi:10.1111/j.1365-313X.2009.04102.x."},"publication":"Plant Journal","doi":"10.1111/j.1365-313X.2009.04102.x","date_published":"2010-03-01T00:00:00Z","month":"03","day":"01","publisher":"Wiley-Blackwell","intvolume":" 61","title":"Probing plant membranes with FM dyes: Tracking dragging or blocking?","status":"public","publication_status":"published","_id":"3067","year":"2010","volume":61,"date_updated":"2021-01-12T07:40:49Z","date_created":"2018-12-11T12:01:10Z","author":[{"full_name":"Jelínková, Adriana","last_name":"Jelínková","first_name":"Adriana"},{"last_name":"Malínská","first_name":"Kateřina","full_name":"Malínská, Kateřina"},{"full_name":"Sibu Simon","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu"},{"first_name":"Jürgen","last_name":"Kleine Vehn","full_name":"Kleine-Vehn, Jürgen"},{"first_name":"Markéta","last_name":"Pařezová","full_name":"Pařezová, Markéta"},{"first_name":"Přemysl","last_name":"Pejchar","full_name":"Pejchar, Přemysl"},{"first_name":"Martin","last_name":"Kubeš","full_name":"Kubeš, Martin"},{"full_name":"Martinec, Jan","first_name":"Jan","last_name":"Martinec"},{"full_name":"Jirí Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"}],"type":"journal_article","extern":1,"issue":"5","publist_id":"3635","abstract":[{"lang":"eng","text":"Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity (lipophilicity) of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using simultaneous labelling with FM dyes."}]},{"type":"journal_article","extern":1,"issue":"11","publist_id":"3652","abstract":[{"text":"Plant development is governed by signaling molecules called phytohormones. Typically, in certain developmental processes more than 1 hormone is implicated and, thus, coordination of their overlapping activities is crucial for correct plant development. However, molecular mechanisms underlying the hormonal crosstalk are only poorly understood. Multiple hormones including cytokinin and auxin have been implicated in the regulation of root development. Here we dissect the roles of cytokinin in modulating growth of the primary root. We show that cytokinin effect on root elongation occurs through ethylene signaling whereas cytokinin effect on the root meristem size involves ethylene-independent modulation of transport-dependent asymmetric auxin distribution. Exogenous or endogenous modification of cytokinin levels and cytokinin signaling lead to specific changes in transcription of several auxin efflux carrier genes from the PIN family having a direct impact on auxin efflux from cultured cells and on auxin distribution in the root apex. We propose a novel model for cytokinin action in regulating root growth: Cytokinin influences cell-to-cell auxin transport by modification of expression of several auxin transport components and thus modulates auxin distribution important for regulation of activity and size of the root meristem.","lang":"eng"}],"intvolume":" 106","publisher":"National Academy of Sciences","title":"Cytokinin regulates root meristem activity via modulation of the polar auxin transport","status":"public","publication_status":"published","year":"2009","_id":"3050","volume":106,"date_updated":"2021-01-12T07:40:42Z","date_created":"2018-12-11T12:01:04Z","author":[{"last_name":"Růžička","first_name":"Kamil","full_name":"Růžička, Kamil"},{"last_name":"Šimášková","first_name":"Mária","full_name":"Šimášková, Mária"},{"full_name":"Duclercq, Jérôme","first_name":"Jérôme","last_name":"Duclercq"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"last_name":"Zažímalová","first_name":"Eva","full_name":"Zažímalová, Eva"},{"full_name":"Sibu Simon","first_name":"Sibu","last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1998-6741"},{"full_name":"Jirí Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí"},{"last_name":"Van Montagu","first_name":"Marc","full_name":"Van Montagu, Marc C"},{"full_name":"Eva Benková","first_name":"Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739"}],"day":"17","month":"03","page":"4284 - 4289","quality_controlled":0,"citation":{"chicago":"Růžička, Kamil, Mária Šimášková, Jérôme Duclercq, Jan Petrášek, Eva Zažímalová, Sibu Simon, Jiří Friml, Marc Van Montagu, and Eva Benková. “Cytokinin Regulates Root Meristem Activity via Modulation of the Polar Auxin Transport.” PNAS. National Academy of Sciences, 2009. https://doi.org/10.1073/pnas.0900060106.","short":"K. Růžička, M. Šimášková, J. Duclercq, J. Petrášek, E. Zažímalová, S. Simon, J. Friml, M. Van Montagu, E. Benková, PNAS 106 (2009) 4284–4289.","mla":"Růžička, Kamil, et al. “Cytokinin Regulates Root Meristem Activity via Modulation of the Polar Auxin Transport.” PNAS, vol. 106, no. 11, National Academy of Sciences, 2009, pp. 4284–89, doi:10.1073/pnas.0900060106.","ieee":"K. Růžička et al., “Cytokinin regulates root meristem activity via modulation of the polar auxin transport,” PNAS, vol. 106, no. 11. National Academy of Sciences, pp. 4284–4289, 2009.","apa":"Růžička, K., Šimášková, M., Duclercq, J., Petrášek, J., Zažímalová, E., Simon, S., … Benková, E. (2009). Cytokinin regulates root meristem activity via modulation of the polar auxin transport. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.0900060106","ista":"Růžička K, Šimášková M, Duclercq J, Petrášek J, Zažímalová E, Simon S, Friml J, Van Montagu M, Benková E. 2009. Cytokinin regulates root meristem activity via modulation of the polar auxin transport. PNAS. 106(11), 4284–4289.","ama":"Růžička K, Šimášková M, Duclercq J, et al. Cytokinin regulates root meristem activity via modulation of the polar auxin transport. PNAS. 2009;106(11):4284-4289. doi:10.1073/pnas.0900060106"},"publication":"PNAS","doi":"10.1073/pnas.0900060106","date_published":"2009-03-17T00:00:00Z"}]