[{"quality_controlled":"1","language":[{"iso":"eng"}],"OA_place":"publisher","department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"DOAJ_listed":"1","oa":1,"oa_version":"Published Version","doi":"10.3389/fpls.2025.1612366","date_created":"2025-07-27T22:01:26Z","publication_identifier":{"eissn":["1664-462X"]},"_id":"20080","acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","scopus_import":"1","citation":{"apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., &#38; Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>","ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2025. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>.","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. 2025;16. doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>, vol. 16, 1612366, Frontiers Media, 2025, doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>.","ieee":"M. Gallemi <i>et al.</i>, “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” <i>Frontiers in Plant Science</i>, vol. 16. Frontiers Media, 2025.","short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025)."},"month":"07","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"project":[{"name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7","grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425"},{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"}],"external_id":{"isi":["001530690900001"],"pmid":["40688689"]},"article_processing_charge":"Yes","file_date_updated":"2025-07-31T07:28:54Z","abstract":[{"lang":"eng","text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction."}],"publication":"Frontiers in Plant Science","volume":16,"ec_funded":1,"article_number":"1612366","title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","day":"04","PlanS_conform":"1","year":"2025","file":[{"checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","date_created":"2025-07-31T07:28:54Z","relation":"main_file","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2025-07-31T07:28:54Z","file_size":3665187,"file_name":"2025_FrontiersPlantSc_Gallemi.pdf","file_id":"20093","success":1}],"status":"public","publication_status":"published","pmid":1,"article_type":"original","publisher":"Frontiers Media","date_updated":"2026-05-20T07:53:03Z","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        16","has_accepted_license":"1","ddc":["580"],"APC_amount":"3642,79 EUR","date_published":"2025-07-04T00:00:00Z","corr_author":"1","OA_type":"gold","author":[{"orcid":"0000-0003-4675-6893","id":"460C6802-F248-11E8-B48F-1D18A9856A87","full_name":"Gallemi, Marçal","last_name":"Gallemi","first_name":"Marçal"},{"orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","last_name":"Montesinos López"},{"full_name":"Zarevski, Nikola","id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a","first_name":"Nikola","last_name":"Zarevski"},{"full_name":"Pribyl, Jan","last_name":"Pribyl","first_name":"Jan"},{"first_name":"Petr","last_name":"Skládal","full_name":"Skládal, Petr"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739"}]},{"day":"01","year":"2021","file":[{"access_level":"open_access","checksum":"fad13c509b53bb7a2bef9c946a7ca60a","relation":"main_file","date_created":"2022-05-13T09:00:29Z","file_size":14137503,"content_type":"application/pdf","date_updated":"2022-05-13T09:00:29Z","creator":"dernst","file_id":"11372","file_name":"2021_eLife_Marconi.pdf","success":1}],"status":"public","publication_status":"published","pmid":1,"publisher":"eLife Sciences Publications","article_type":"original","date_updated":"2023-08-14T11:49:23Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"intvolume":"        10","has_accepted_license":"1","ddc":["570"],"date_published":"2021-11-01T00:00:00Z","author":[{"first_name":"Marco","last_name":"Marconi","full_name":"Marconi, Marco"},{"first_name":"Marçal","last_name":"Gallemi","full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4675-6893"},{"first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Krzysztof","last_name":"Wabnik","full_name":"Wabnik, Krzysztof"}],"quality_controlled":"1","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"oa_version":"Published Version","oa":1,"doi":"10.7554/elife.72132","date_created":"2021-11-11T10:05:18Z","_id":"10270","publication_identifier":{"issn":["2050-084X"]},"acknowledgement":"e are grateful Richard Smith, Anne-Lise Routier, Crisanto Gutierrez and Juergen Kleine-Vehn for providing critical comments on the manuscript. Funding: This work was supported by the Programa de Atraccion de Talento 2017 (Comunidad de Madrid, 2017-T1/BIO-5654 to KW), Severo Ochoa (SO) Programme for Centres of Excellence in R&D from the Agencia Estatal de Investigacion of Spain (grant SEV-2016–0672 (2017–2021) to KW via the CBGP). In the frame of SEV-2016–0672 funding MM is supported with a postdoctoral contract. KW was supported by Programa Estatal de Generacion del Conocimiento y Fortalecimiento Cientıfico y Tecnologico del Sistema de I + D + I 2019 (PGC2018-093387-A-I00) from MICIU (to KW). MG is recipient of an IST Interdisciplinary Project (IC1022IPC03).","scopus_import":"1","citation":{"apa":"Marconi, M., Gallemi, M., Benková, E., &#38; Wabnik, K. (2021). A coupled mechano-biochemical model for cell polarity guided anisotropic root growth. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.72132\">https://doi.org/10.7554/elife.72132</a>","ista":"Marconi M, Gallemi M, Benková E, Wabnik K. 2021. A coupled mechano-biochemical model for cell polarity guided anisotropic root growth. eLife. 10, 72132.","chicago":"Marconi, Marco, Marçal Gallemi, Eva Benková, and Krzysztof Wabnik. “A Coupled Mechano-Biochemical Model for Cell Polarity Guided Anisotropic Root Growth.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/elife.72132\">https://doi.org/10.7554/elife.72132</a>.","ama":"Marconi M, Gallemi M, Benková E, Wabnik K. A coupled mechano-biochemical model for cell polarity guided anisotropic root growth. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/elife.72132\">10.7554/elife.72132</a>","mla":"Marconi, Marco, et al. “A Coupled Mechano-Biochemical Model for Cell Polarity Guided Anisotropic Root Growth.” <i>ELife</i>, vol. 10, 72132, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/elife.72132\">10.7554/elife.72132</a>.","ieee":"M. Marconi, M. Gallemi, E. Benková, and K. Wabnik, “A coupled mechano-biochemical model for cell polarity guided anisotropic root growth,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.","short":"M. Marconi, M. Gallemi, E. Benková, K. Wabnik, ELife 10 (2021)."},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"11","type":"journal_article","article_processing_charge":"Yes","external_id":{"pmid":["34723798"],"isi":["000734671200001"]},"file_date_updated":"2022-05-13T09:00:29Z","publication":"eLife","abstract":[{"lang":"eng","text":"Plants develop new organs to adjust their bodies to dynamic changes in the environment. How independent organs achieve anisotropic shapes and polarities is poorly understood. To address this question, we constructed a mechano-biochemical model for Arabidopsis root meristem growth that integrates biologically plausible principles. Computer model simulations demonstrate how differential growth of neighboring tissues results in the initial symmetry-breaking leading to anisotropic root growth. Furthermore, the root growth feeds back on a polar transport network of the growth regulator auxin. Model, predictions are in close agreement with in vivo patterns of anisotropic growth, auxin distribution, and cell polarity, as well as several root phenotypes caused by chemical, mechanical, or genetic perturbations. Our study demonstrates that the combination of tissue mechanics and polar auxin transport organizes anisotropic root growth and cell polarities during organ outgrowth. Therefore, a mobile auxin signal transported through immobile cells drives polarity and growth mechanics to coordinate complex organ development."}],"volume":10,"title":"A coupled mechano-biochemical model for cell polarity guided anisotropic root growth","article_number":"72132"},{"file":[{"content_type":"application/pdf","date_updated":"2021-09-07T09:04:53Z","file_size":2162247,"creator":"cchlebak","access_level":"open_access","checksum":"6b7055cf89f1b7ed8594c3fdf56f000b","relation":"main_file","date_created":"2021-09-06T12:50:19Z","file_id":"9988","file_name":"2021_IntJMolecularSciences_Velasquez.pdf"}],"day":"26","year":"2021","article_type":"original","publisher":"MDPI","date_updated":"2024-10-09T21:00:50Z","status":"public","pmid":1,"publication_status":"published","keyword":["auxin","growth","cell wall","xyloglucans","hypocotyls","gravitropism"],"has_accepted_license":"1","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        22","author":[{"full_name":"Velasquez, Silvia Melina","last_name":"Velasquez","first_name":"Silvia Melina"},{"full_name":"Guo, Xiaoyuan","last_name":"Guo","first_name":"Xiaoyuan"},{"orcid":"0000-0003-4675-6893","id":"460C6802-F248-11E8-B48F-1D18A9856A87","full_name":"Gallemi, Marçal","last_name":"Gallemi","first_name":"Marçal"},{"full_name":"Aryal, Bibek","first_name":"Bibek","last_name":"Aryal"},{"last_name":"Venhuizen","first_name":"Peter","full_name":"Venhuizen, Peter"},{"full_name":"Barbez, Elke","last_name":"Barbez","first_name":"Elke"},{"first_name":"Kai Alexander","last_name":"Dünser","full_name":"Dünser, Kai Alexander"},{"full_name":"Darino, Martin","last_name":"Darino","first_name":"Martin"},{"first_name":"Aleš","last_name":"Pӗnčík","full_name":"Pӗnčík, Aleš"},{"last_name":"Novák","first_name":"Ondřej","full_name":"Novák, Ondřej"},{"first_name":"Maria","last_name":"Kalyna","full_name":"Kalyna, Maria"},{"full_name":"Mouille, Gregory","first_name":"Gregory","last_name":"Mouille"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková"},{"full_name":"Bhalerao, Rishikesh P.","last_name":"Bhalerao","first_name":"Rishikesh P."},{"full_name":"Mravec, Jozef","last_name":"Mravec","first_name":"Jozef"},{"full_name":"Kleine-Vehn, Jürgen","first_name":"Jürgen","last_name":"Kleine-Vehn"}],"ddc":["575"],"date_published":"2021-08-26T00:00:00Z","corr_author":"1","department":[{"_id":"EvBe"}],"oa":1,"oa_version":"Published Version","issue":"17","quality_controlled":"1","language":[{"iso":"eng"}],"scopus_import":"1","citation":{"short":"S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A. Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P. Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences 22 (2021).","ieee":"S. M. Velasquez <i>et al.</i>, “Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants,” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17. MDPI, 2021.","mla":"Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17, 9222, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>.","ama":"Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. 2021;22(17). doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>","chicago":"Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>.","ista":"Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA, Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. International Journal of Molecular Sciences. 22(17), 9222.","apa":"Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E., … Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"08","type":"journal_article","doi":"10.3390/ijms22179222","date_created":"2021-09-05T22:01:24Z","publication_identifier":{"issn":["1661-6596"],"eissn":["1422-0067"]},"_id":"9986","acknowledgement":"We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical reading.\r\n","article_processing_charge":"Yes","external_id":{"isi":["000694347100001"],"pmid":["34502129"]},"title":"Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants","article_number":"9222","file_date_updated":"2021-09-07T09:04:53Z","publication":"International Journal of Molecular Sciences","abstract":[{"lang":"eng","text":"Size control is a fundamental question in biology, showing incremental complexity in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a vital growth regulator with central importance for differential growth control. Our results indicate that auxin-reliant growth programs affect the molecular complexity of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent induction and repression of growth coincide with reduced and enhanced molecular complexity of xyloglucans, respectively. In agreement with a proposed function in growth control, genetic interference with xyloglucan side decorations distinctly modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent growth programs have a spatially defined effect on xyloglucan’s molecular structure, which in turn affects cell wall mechanics and specifies differential, gravitropic hypocotyl growth."}],"volume":22},{"project":[{"call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"}],"article_processing_charge":"No","external_id":{"pmid":["32358503"],"isi":["000531425900012"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance","article_number":"2170","file_date_updated":"2020-10-06T07:47:53Z","abstract":[{"lang":"eng","text":"Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a regulator of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens."}],"publication":"Nature Communications","ec_funded":1,"volume":11,"department":[{"_id":"EvBe"}],"oa":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"quality_controlled":"1","scopus_import":"1","citation":{"ista":"Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.","apa":"Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L., … Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>","short":"A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C. Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau, F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann, G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020).","ieee":"A. Hurny <i>et al.</i>, “Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","mla":"Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>, vol. 11, 2170, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>.","ama":"Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>","chicago":"Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>."},"month":"05","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","type":"journal_article","date_created":"2020-05-10T22:00:48Z","doi":"10.1038/s41467-020-15895-5","publication_identifier":{"eissn":["2041-1723"]},"_id":"7805","acknowledgement":"We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing published material, Lesia Rodriguez Solovey for technical assistance. This work was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B., the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"intvolume":"        11","author":[{"last_name":"Hurny","first_name":"Andrej","orcid":"0000-0003-3638-1426","id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Hurny, Andrej"},{"orcid":"0000-0003-1923-2410","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","full_name":"Cuesta, Candela","last_name":"Cuesta","first_name":"Candela"},{"first_name":"Nicola","last_name":"Cavallari","full_name":"Cavallari, Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Krisztina","last_name":"Ötvös","full_name":"Ötvös, Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983"},{"full_name":"Duclercq, Jerome","first_name":"Jerome","last_name":"Duclercq"},{"last_name":"Dokládal","first_name":"Ladislav","full_name":"Dokládal, Ladislav"},{"last_name":"Montesinos López","first_name":"Juan C","orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","full_name":"Montesinos López, Juan C"},{"last_name":"Gallemi","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","full_name":"Gallemi, Marçal","orcid":"0000-0003-4675-6893"},{"last_name":"Semeradova","first_name":"Hana","id":"42FE702E-F248-11E8-B48F-1D18A9856A87","full_name":"Semeradova, Hana"},{"full_name":"Rauter, Thomas","id":"A0385D1A-9376-11EA-A47D-9862C5E3AB22","first_name":"Thomas","last_name":"Rauter"},{"last_name":"Stenzel","first_name":"Irene","full_name":"Stenzel, Irene"},{"first_name":"Geert","last_name":"Persiau","full_name":"Persiau, Geert"},{"last_name":"Benade","first_name":"Freia","full_name":"Benade, Freia"},{"last_name":"Bhalearo","first_name":"Rishikesh","full_name":"Bhalearo, Rishikesh"},{"full_name":"Sýkorová, Eva","last_name":"Sýkorová","first_name":"Eva"},{"last_name":"Gorzsás","first_name":"András","full_name":"Gorzsás, András"},{"first_name":"Julien","last_name":"Sechet","full_name":"Sechet, Julien"},{"last_name":"Mouille","first_name":"Gregory","full_name":"Mouille, Gregory"},{"last_name":"Heilmann","first_name":"Ingo","full_name":"Heilmann, Ingo"},{"first_name":"Geert","last_name":"De Jaeger","full_name":"De Jaeger, Geert"},{"full_name":"Ludwig-Müller, Jutta","last_name":"Ludwig-Müller","first_name":"Jutta"},{"last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"}],"date_published":"2020-05-01T00:00:00Z","ddc":["570"],"corr_author":"1","file":[{"file_id":"8614","file_name":"2020_NatureComm_Hurny.pdf","success":1,"access_level":"open_access","checksum":"2cba327c9e9416d75cb96be54b0fb441","relation":"main_file","date_created":"2020-10-06T07:47:53Z","date_updated":"2020-10-06T07:47:53Z","content_type":"application/pdf","file_size":4743576,"creator":"dernst"}],"day":"01","year":"2020","publisher":"Springer Nature","article_type":"original","date_updated":"2026-04-02T14:32:53Z","status":"public","pmid":1,"publication_status":"published"},{"title":"Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2","article_number":"1680","volume":10,"publication":"Frontiers in Plant Science","abstract":[{"text":"The ability to sense environmental temperature and to coordinate growth and development accordingly, is critical to the reproductive success of plants. Flowering time is regulated at the level of gene expression by a complex network of factors that integrate environmental and developmental cues. One of the main players, involved in modulating flowering time in response to changes in ambient temperature is FLOWERING LOCUS M (FLM). FLM transcripts can undergo extensive alternative splicing producing multiple variants, of which FLM-β and FLM-δ are the most representative. While FLM-β codes for the flowering repressor FLM protein, translation of FLM-δ has the opposite effect on flowering. Here we show that the cyclin-dependent kinase G2 (CDKG2), together with its cognate cyclin, CYCLYN L1 (CYCL1) affects the alternative splicing of FLM, balancing the levels of FLM-β and FLM-δ across the ambient temperature range. In the absence of the CDKG2/CYCL1 complex, FLM-β expression is reduced while FLM-δ is increased in a temperature dependent manner and these changes are associated with an early flowering phenotype in the cdkg2 mutant lines. In addition, we found that transcript variants retaining the full FLM intron 1 are sequestered in the cell nucleus. Strikingly, FLM intron 1 splicing is also regulated by CDKG2/CYCL1. Our results provide evidence that temperature and CDKs regulate the alternative splicing of FLM, contributing to flowering time definition.","lang":"eng"}],"file_date_updated":"2020-07-14T12:47:56Z","article_processing_charge":"No","external_id":{"isi":["000511376000001"],"pmid":["32038671"]},"type":"journal_article","month":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"C. Nibau, M. Gallemi, D. Dadarou, J. H. Doonan, and N. Cavallari, “Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2,” <i>Frontiers in Plant Science</i>, vol. 10. Frontiers Media, 2020.","mla":"Nibau, Candida, et al. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>, vol. 10, 1680, Frontiers Media, 2020, doi:<a href=\"https://doi.org/10.3389/fpls.2019.01680\">10.3389/fpls.2019.01680</a>.","short":"C. Nibau, M. Gallemi, D. Dadarou, J.H. Doonan, N. Cavallari, Frontiers in Plant Science 10 (2020).","chicago":"Nibau, Candida, Marçal Gallemi, Despoina Dadarou, John H. Doonan, and Nicola Cavallari. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2020. <a href=\"https://doi.org/10.3389/fpls.2019.01680\">https://doi.org/10.3389/fpls.2019.01680</a>.","ama":"Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers in Plant Science</i>. 2020;10. doi:<a href=\"https://doi.org/10.3389/fpls.2019.01680\">10.3389/fpls.2019.01680</a>","ista":"Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. 2020. Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. Frontiers in Plant Science. 10, 1680.","apa":"Nibau, C., Gallemi, M., Dadarou, D., Doonan, J. H., &#38; Cavallari, N. (2020). Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2019.01680\">https://doi.org/10.3389/fpls.2019.01680</a>"},"scopus_import":"1","_id":"7350","publication_identifier":{"issn":["1664-462X"]},"date_created":"2020-01-22T15:23:57Z","doi":"10.3389/fpls.2019.01680","oa":1,"oa_version":"Published Version","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"quality_controlled":"1","author":[{"full_name":"Nibau, Candida","first_name":"Candida","last_name":"Nibau"},{"first_name":"Marçal","last_name":"Gallemi","full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4675-6893"},{"full_name":"Dadarou, Despoina","last_name":"Dadarou","first_name":"Despoina"},{"full_name":"Doonan, John H.","last_name":"Doonan","first_name":"John H."},{"last_name":"Cavallari","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola"}],"corr_author":"1","date_published":"2020-01-22T00:00:00Z","ddc":["580"],"has_accepted_license":"1","intvolume":"        10","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"date_updated":"2025-06-12T07:33:02Z","publisher":"Frontiers Media","article_type":"original","publication_status":"published","pmid":1,"status":"public","file":[{"creator":"dernst","file_size":1951438,"date_updated":"2020-07-14T12:47:56Z","content_type":"application/pdf","relation":"main_file","date_created":"2020-01-27T09:07:02Z","checksum":"d1f92e60a713fbd15097ce895e5c7ccb","access_level":"open_access","file_name":"2020_FrontiersPlantScience_Nibau.pdf","file_id":"7366"}],"year":"2020","day":"22"},{"oa_version":"Published Version","oa":1,"department":[{"_id":"EvBe"}],"issue":"17","language":[{"iso":"eng"}],"quality_controlled":"1","month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","scopus_import":"1","citation":{"apa":"Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., &#38; Benková, E. (2019). Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>","ista":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. Development. 146(17), dev175919.","chicago":"Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad, and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>. The Company of Biologists, 2019. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>.","ama":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. 2019;146(17). doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>","mla":"Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>, vol. 146, no. 17, dev175919, The Company of Biologists, 2019, doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>.","ieee":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková, “Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis,” <i>Development</i>, vol. 146, no. 17. The Company of Biologists, 2019.","short":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development 146 (2019)."},"acknowledgement":"We thank Jiri Friml and Phillip Brewer for inspiring discussion and for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738).","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/dev.175919"}],"date_created":"2019-09-22T22:00:36Z","doi":"10.1242/dev.175919","_id":"6897","publication_identifier":{"eissn":["1477-9129"]},"external_id":{"pmid":["31391194"],"isi":["000486297400011"]},"article_processing_charge":"No","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362","call_identifier":"FP7","name":"Hormonal cross-talk in plant organogenesis"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"title":"Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis","article_number":"dev175919","abstract":[{"text":"The apical hook is a transiently formed structure that plays a protective role when the germinating seedling penetrates through the soil towards the surface. Crucial for proper bending is the local auxin maxima, which defines the concave (inner) side of the hook curvature. As no sign of asymmetric auxin distribution has been reported in embryonic hypocotyls prior to hook formation, the question of how auxin asymmetry is established in the early phases of seedling germination remains largely unanswered. Here, we analyzed the auxin distribution and expression of PIN auxin efflux carriers from early phases of germination, and show that bending of the root in response to gravity is the crucial initial cue that governs the hypocotyl bending required for apical hook formation. Importantly, polar auxin transport machinery is established gradually after germination starts as a result of tight root-hypocotyl interaction and a proper balance between abscisic acid and gibberellins.","lang":"eng"}],"publication":"Development","ec_funded":1,"volume":146,"year":"2019","day":"12","date_updated":"2026-06-18T19:13:37Z","publisher":"The Company of Biologists","article_type":"original","status":"public","pmid":1,"publication_status":"published","intvolume":"       146","isi":1,"author":[{"id":"40A4B9E6-F248-11E8-B48F-1D18A9856A87","full_name":"Zhu, Qiang","last_name":"Zhu","first_name":"Qiang"},{"last_name":"Gallemi","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","full_name":"Gallemi, Marçal","orcid":"0000-0003-4675-6893"},{"first_name":"Jiří","last_name":"Pospíšil","full_name":"Pospíšil, Jiří"},{"full_name":"Žádníková, Petra","first_name":"Petra","last_name":"Žádníková"},{"full_name":"Strnad, Miroslav","last_name":"Strnad","first_name":"Miroslav"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková"}],"ddc":["580"],"date_published":"2019-09-12T00:00:00Z"},{"title":"DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis","volume":143,"publication":"Development","abstract":[{"text":"When plants grow in close proximity basic resources such as light can become limiting. Under such conditions plants respond to anticipate and/or adapt to the light shortage, a process known as the shade avoidance syndrome (SAS). Following genetic screening using a shade-responsive luciferase reporter line (PHYB:LUC), we identified DRACULA2 (DRA2), which encodes an Arabidopsis homolog of mammalian nucleoporin 98, a component of the nuclear pore complex (NPC). DRA2, together with other nucleoporins, participates positively in the control of the hypocotyl elongation response to plant proximity, a role that can be considered dependent on the nucleocytoplasmic transport of macromolecules (i.e. is transport dependent). In addition, our results reveal a specific role for DRA2 in controlling shade-induced gene expression. We suggest that this novel regulatory role of DRA2 is transport independent and that it might rely on its dynamic localization within and outside of the NPC. These results provide mechanistic insights in to how SAS responses are rapidly established by light conditions. They also indicate that nucleoporins have an active role in plant signaling.","lang":"eng"}],"page":"1623 - 1631","article_processing_charge":"No","external_id":{"isi":["000389588100018"]},"type":"journal_article","month":"05","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"ama":"Gallemi M, Galstyan A, Paulišić S, et al. DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis. <i>Development</i>. 2016;143(9):1623-1631. doi:<a href=\"https://doi.org/10.1242/dev.130211\">10.1242/dev.130211</a>","chicago":"Gallemi, Marçal, Anahit Galstyan, Sandi Paulišić, Christiane Then, Almudena Ferrández Ayela, Laura Lorenzo Orts, Irma Roig Villanova, et al. “DRACULA2 Is a Dynamic Nucleoporin with a Role in Regulating the Shade Avoidance Syndrome in Arabidopsis.” <i>Development</i>. Company of Biologists, 2016. <a href=\"https://doi.org/10.1242/dev.130211\">https://doi.org/10.1242/dev.130211</a>.","short":"M. Gallemi, A. Galstyan, S. Paulišić, C. Then, A. Ferrández Ayela, L. Lorenzo Orts, I. Roig Villanova, X. Wang, J. Micol, M. Ponce, P. Devlin, J. Martínez García, Development 143 (2016) 1623–1631.","ieee":"M. Gallemi <i>et al.</i>, “DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis,” <i>Development</i>, vol. 143, no. 9. Company of Biologists, pp. 1623–1631, 2016.","mla":"Gallemi, Marçal, et al. “DRACULA2 Is a Dynamic Nucleoporin with a Role in Regulating the Shade Avoidance Syndrome in Arabidopsis.” <i>Development</i>, vol. 143, no. 9, Company of Biologists, 2016, pp. 1623–31, doi:<a href=\"https://doi.org/10.1242/dev.130211\">10.1242/dev.130211</a>.","apa":"Gallemi, M., Galstyan, A., Paulišić, S., Then, C., Ferrández Ayela, A., Lorenzo Orts, L., … Martínez García, J. (2016). DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.130211\">https://doi.org/10.1242/dev.130211</a>","ista":"Gallemi M, Galstyan A, Paulišić S, Then C, Ferrández Ayela A, Lorenzo Orts L, Roig Villanova I, Wang X, Micol J, Ponce M, Devlin P, Martínez García J. 2016. DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis. Development. 143(9), 1623–1631."},"scopus_import":"1","acknowledgement":"M.G. received an FPI fellowship from the Spanish Ministerio de Economía y Competitividad (MINECO). A.G. and A.F.-A. received FPU fellowships from the Spanish Ministerio de Educación. S.P. received an FI fellowship from the Agència de Gestió D'ajuts Universitaris i de Recerca (AGAUR - Generalitat de Catalunya). C.T. received a Marie Curie IEF postdoctoral contract funded by the European Commission. I.R.-V. received initially an FPI fellowship from the Spanish MINECO and later a Beatriu de Pinós contract from AGAUR. Our research is supported by grants from the Spanish MINECO-FEDER [BIO2008-00169, BIO2011-23489 and BIO2014-59895-P] and Generalitat de Catalunya [2011-SGR447 and Xarba] to J.F.M.-G., and Generalitat Valenciana [PROMETEO/2009/112, PROMETEOII/2014/006] to M.R.P. and J.L.M. We acknowledge the support of the Spanish MINECO for the ‘Centro de Excelencia Severo Ochoa 2016-2019’ [award SEV-2015-0533]. We thank the CRAG greenhouse service for plant care; Chus Burillo for technical help; Sergi Portolés and Carles Rentero for assistance with mutagenesis; Mark Estelle (UCSD, USA) for providing sar1-4, sar3-1 and sar3-3 seeds; Juanjo López-Moya (CRAG, Barcelona; 35S:HcPro plasmid) and Dolors Ludevid (CRAG; C307 plasmid) for providing DNA plasmids; and Manuel Rodríguez-Concepción (CRAG) and Miguel Blázquez (IBMCP, Valencia, Spain) for comments on the manuscript.","_id":"1258","doi":"10.1242/dev.130211","date_created":"2018-12-11T11:50:59Z","oa_version":"None","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"9","author":[{"last_name":"Gallemi Rovira","first_name":"Marcal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","full_name":"Gallemi Rovira, Marcal","orcid":"0000-0003-4675-6893"},{"full_name":"Galstyan, Anahit","first_name":"Anahit","last_name":"Galstyan"},{"last_name":"Paulišić","first_name":"Sandi","full_name":"Paulišić, Sandi"},{"full_name":"Then, Christiane","first_name":"Christiane","last_name":"Then"},{"full_name":"Ferrández Ayela, Almudena","first_name":"Almudena","last_name":"Ferrández Ayela"},{"last_name":"Lorenzo Orts","first_name":"Laura","full_name":"Lorenzo Orts, Laura"},{"first_name":"Irma","last_name":"Roig Villanova","full_name":"Roig Villanova, Irma"},{"first_name":"Xuewen","last_name":"Wang","full_name":"Wang, Xuewen"},{"first_name":"José","last_name":"Micol","full_name":"Micol, José"},{"last_name":"Ponce","first_name":"Maria","full_name":"Ponce, Maria"},{"first_name":"Paul","last_name":"Devlin","full_name":"Devlin, Paul"},{"first_name":"Jaime","last_name":"Martínez García","full_name":"Martínez García, Jaime"}],"publist_id":"6068","date_published":"2016-05-03T00:00:00Z","intvolume":"       143","isi":1,"date_updated":"2025-09-22T09:05:00Z","publisher":"Company of Biologists","publication_status":"published","status":"public","year":"2016","day":"03"},{"publication_status":"published","status":"public","date_updated":"2025-09-22T08:58:05Z","publisher":"American Association for the Advancement of Science","year":"2016","day":"05","date_published":"2016-07-05T00:00:00Z","publist_id":"6057","author":[{"last_name":"Elsayad","first_name":"Kareem","full_name":"Elsayad, Kareem"},{"full_name":"Werner, Stephanie","first_name":"Stephanie","last_name":"Werner"},{"orcid":"0000-0003-4675-6893","full_name":"Gallemi Rovira, Marcal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","first_name":"Marcal","last_name":"Gallemi Rovira"},{"full_name":"Kong, Jixiang","last_name":"Kong","first_name":"Jixiang"},{"first_name":"Edmundo","last_name":"Guajardo","full_name":"Guajardo, Edmundo"},{"first_name":"Lijuan","last_name":"Zhang","full_name":"Zhang, Lijuan"},{"full_name":"Jaillais, Yvon","last_name":"Jaillais","first_name":"Yvon"},{"full_name":"Greb, Thomas","first_name":"Thomas","last_name":"Greb"},{"first_name":"Youssef","last_name":"Belkhadir","full_name":"Belkhadir, Youssef"}],"intvolume":"         9","isi":1,"_id":"1265","date_created":"2018-12-11T11:51:02Z","doi":"10.1126/scisignal.aaf6326","type":"journal_article","month":"07","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"ista":"Elsayad K, Werner S, Gallemi M, Kong J, Guajardo E, Zhang L, Jaillais Y, Greb T, Belkhadir Y. 2016. Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. Science Signaling. 9(435), rs5.","apa":"Elsayad, K., Werner, S., Gallemi, M., Kong, J., Guajardo, E., Zhang, L., … Belkhadir, Y. (2016). Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. <i>Science Signaling</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/scisignal.aaf6326\">https://doi.org/10.1126/scisignal.aaf6326</a>","short":"K. Elsayad, S. Werner, M. Gallemi, J. Kong, E. Guajardo, L. Zhang, Y. Jaillais, T. Greb, Y. Belkhadir, Science Signaling 9 (2016).","mla":"Elsayad, Kareem, et al. “Mapping the Subcellular Mechanical Properties of Live Cells in Tissues with Fluorescence Emission-Brillouin Imaging.” <i>Science Signaling</i>, vol. 9, no. 435, rs5, American Association for the Advancement of Science, 2016, doi:<a href=\"https://doi.org/10.1126/scisignal.aaf6326\">10.1126/scisignal.aaf6326</a>.","ieee":"K. Elsayad <i>et al.</i>, “Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging,” <i>Science Signaling</i>, vol. 9, no. 435. American Association for the Advancement of Science, 2016.","ama":"Elsayad K, Werner S, Gallemi M, et al. Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. <i>Science Signaling</i>. 2016;9(435). doi:<a href=\"https://doi.org/10.1126/scisignal.aaf6326\">10.1126/scisignal.aaf6326</a>","chicago":"Elsayad, Kareem, Stephanie Werner, Marçal Gallemi, Jixiang Kong, Edmundo Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb, and Youssef Belkhadir. “Mapping the Subcellular Mechanical Properties of Live Cells in Tissues with Fluorescence Emission-Brillouin Imaging.” <i>Science Signaling</i>. American Association for the Advancement of Science, 2016. <a href=\"https://doi.org/10.1126/scisignal.aaf6326\">https://doi.org/10.1126/scisignal.aaf6326</a>."},"scopus_import":"1","language":[{"iso":"eng"}],"quality_controlled":"1","issue":"435","oa_version":"None","department":[{"_id":"EvBe"}],"volume":9,"abstract":[{"lang":"eng","text":"Extracellular matrices (ECMs) are central to the advent of multicellular life, and their mechanical propertiesare modulated by and impinge on intracellular signaling pathways that regulate vital cellular functions. High spatial-resolution mapping of mechanical properties in live cells is, however, extremely challenging. Thus, our understanding of how signaling pathways process physiological signals to generate appropriate mechanical responses is limited. We introduce fluorescence emission-Brillouin scattering imaging (FBi), a method for the parallel and all-optical measurements of mechanical properties and fluorescence at the submicrometer scale in living organisms. Using FBi, we showed thatchanges in cellular hydrostatic pressure and cytoplasm viscoelasticity modulate the mechanical signatures of plant ECMs. We further established that the measured &quot;stiffness&quot; of plant ECMs is symmetrically patternedin hypocotyl cells undergoing directional growth. Finally, application of this method to Arabidopsis thaliana with photoreceptor mutants revealed that red and far-red light signals are essential modulators of ECM viscoelasticity. By mapping the viscoelastic signatures of a complex ECM, we provide proof of principlefor the organism-wide applicability of FBi for measuring the mechanical outputs of intracellular signaling pathways. As such, our work has implications for investigations of mechanosignaling pathways and developmental biology."}],"publication":"Science Signaling","title":"Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging","article_number":"rs5","article_processing_charge":"No","external_id":{"isi":["000380778300003"]}}]