---
OA_place: publisher
_id: '20485'
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "I would also like to acknowledge the funding that I received from
  the European Union’s\r\nHorizon 2020 research and Innovation programme under the
  Marie Sklodowska-Curie\r\nGrant Agreement No. 665385. This work would not have been
  possible without the contribution and support of people\r\nbehind the scientific
  service units at ISTA: the Life Science Facility (LSF), Imaging and\r\nOptics Facility
  (IOF), the Bioinformatics Unit, Protein Services Unit and\r\nElectrophysiology Unit.
  I would also like to recognize the work of people at the Vienna\r\nBiocenter (VBC)
  Mass Spectrometry Facility, particularly Markus Hartl and WeiQiang\r\nChen. "
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Michaela
  full_name: Misova, Michaela
  id: 495A3C32-F248-11E8-B48F-1D18A9856A87
  last_name: Misova
  orcid: 0000-0003-2427-6856
citation:
  ama: Misova M. Dissecting gap junction biology using the C. elegans nervous system.
    2025. doi:<a href="https://doi.org/10.15479/AT-ISTA-20485">10.15479/AT-ISTA-20485</a>
  apa: Misova, M. (2025). <i>Dissecting gap junction biology using the C. elegans
    nervous system</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT-ISTA-20485">https://doi.org/10.15479/AT-ISTA-20485</a>
  chicago: Misova, Michaela. “Dissecting Gap Junction Biology Using the C. Elegans
    Nervous System.” Institute of Science and Technology Austria, 2025. <a href="https://doi.org/10.15479/AT-ISTA-20485">https://doi.org/10.15479/AT-ISTA-20485</a>.
  ieee: M. Misova, “Dissecting gap junction biology using the C. elegans nervous system,”
    Institute of Science and Technology Austria, 2025.
  ista: Misova M. 2025. Dissecting gap junction biology using the C. elegans nervous
    system. Institute of Science and Technology Austria.
  mla: Misova, Michaela. <i>Dissecting Gap Junction Biology Using the C. Elegans Nervous
    System</i>. Institute of Science and Technology Austria, 2025, doi:<a href="https://doi.org/10.15479/AT-ISTA-20485">10.15479/AT-ISTA-20485</a>.
  short: M. Misova, Dissecting Gap Junction Biology Using the C. Elegans Nervous System,
    Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-10-17T16:15:09Z
date_published: 2025-10-23T00:00:00Z
date_updated: 2026-04-07T11:54:00Z
day: '23'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: MaDe
doi: 10.15479/AT-ISTA-20485
ec_funded: 1
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  date_created: 2025-10-23T08:22:35Z
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  date_updated: 2025-10-23T08:21:21Z
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file_date_updated: 2025-11-06T11:08:06Z
has_accepted_license: '1'
language:
- iso: eng
month: '10'
oa_version: Published Version
page: '155'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
publication_identifier:
  isbn:
  - 978-3-99078-068-8
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Mario
  full_name: de Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: de Bono
  orcid: 0000-0001-8347-0443
title: Dissecting gap junction biology using the C. elegans nervous system
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
_id: '6187'
abstract:
- lang: eng
  text: Aberrant display of the truncated core1 O-glycan T-antigen is a common feature
    of human cancer cells that correlates with metastasis. Here we show that T-antigen
    in Drosophila melanogaster macrophages is involved in their developmentally programmed
    tissue invasion. Higher macrophage T-antigen levels require an atypical major
    facilitator superfamily (MFS) member that we named Minerva which enables macrophage
    dissemination and invasion. We characterize for the first time the T and Tn glycoform
    O-glycoproteome of the Drosophila melanogaster embryo, and determine that Minerva
    increases the presence of T-antigen on proteins in pathways previously linked
    to cancer, most strongly on the sulfhydryl oxidase Qsox1 which we show is required
    for macrophage tissue entry. Minerva’s vertebrate ortholog, MFSD1, rescues the
    minerva mutant’s migration and T-antigen glycosylation defects. We thus identify
    a key conserved regulator that orchestrates O-glycosylation on a protein subset
    to activate a program governing migration steps important for both development
    and cancer metastasis.
acknowledged_ssus:
- _id: LifeSc
article_number: e41801
article_processing_charge: No
author:
- first_name: Katarina
  full_name: Valosková, Katarina
  id: 46F146FC-F248-11E8-B48F-1D18A9856A87
  last_name: Valosková
  orcid: 0000-0002-7926-0221
- first_name: Julia
  full_name: Biebl, Julia
  id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
  last_name: Biebl
- first_name: Marko
  full_name: Roblek, Marko
  id: 3047D808-F248-11E8-B48F-1D18A9856A87
  last_name: Roblek
  orcid: 0000-0001-9588-1389
- first_name: Shamsi
  full_name: Emtenani, Shamsi
  id: 49D32318-F248-11E8-B48F-1D18A9856A87
  last_name: Emtenani
  orcid: 0000-0001-6981-6938
- first_name: Attila
  full_name: György, Attila
  id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
  last_name: György
  orcid: 0000-0002-1819-198X
- first_name: Michaela
  full_name: Misova, Michaela
  id: 495A3C32-F248-11E8-B48F-1D18A9856A87
  last_name: Misova
  orcid: 0000-0003-2427-6856
- first_name: Aparna
  full_name: Ratheesh, Aparna
  id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
  last_name: Ratheesh
  orcid: 0000-0001-7190-0776
- first_name: Patricia
  full_name: Dos Reis Rodrigues, Patricia
  id: 26E95904-5160-11E9-9C0B-C5B0DC97E90F
  last_name: Dos Reis Rodrigues
  orcid: 0000-0003-1681-508X
- first_name: Katerina
  full_name: Shkarina, Katerina
  last_name: Shkarina
- first_name: Ida Signe Bohse
  full_name: Larsen, Ida Signe Bohse
  last_name: Larsen
- first_name: Sergey Y
  full_name: Vakhrushev, Sergey Y
  last_name: Vakhrushev
- first_name: Henrik
  full_name: Clausen, Henrik
  last_name: Clausen
- first_name: Daria E
  full_name: Siekhaus, Daria E
  id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
  last_name: Siekhaus
  orcid: 0000-0001-8323-8353
citation:
  ama: Valosková K, Bicher J, Roblek M, et al. A conserved major facilitator superfamily
    member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion.
    <i>eLife</i>. 2019;8. doi:<a href="https://doi.org/10.7554/elife.41801">10.7554/elife.41801</a>
  apa: Valosková, K., Bicher, J., Roblek, M., Emtenani, S., György, A., Misova, M.,
    … Siekhaus, D. E. (2019). A conserved major facilitator superfamily member orchestrates
    a subset of O-glycosylation to aid macrophage tissue invasion. <i>ELife</i>. eLife
    Sciences Publications. <a href="https://doi.org/10.7554/elife.41801">https://doi.org/10.7554/elife.41801</a>
  chicago: Valosková, Katarina, Julia Bicher, Marko Roblek, Shamsi Emtenani, Attila
    György, Michaela Misova, Aparna Ratheesh, et al. “A Conserved Major Facilitator
    Superfamily Member Orchestrates a Subset of O-Glycosylation to Aid Macrophage
    Tissue Invasion.” <i>ELife</i>. eLife Sciences Publications, 2019. <a href="https://doi.org/10.7554/elife.41801">https://doi.org/10.7554/elife.41801</a>.
  ieee: K. Valosková <i>et al.</i>, “A conserved major facilitator superfamily member
    orchestrates a subset of O-glycosylation to aid macrophage tissue invasion,” <i>eLife</i>,
    vol. 8. eLife Sciences Publications, 2019.
  ista: Valosková K, Bicher J, Roblek M, Emtenani S, György A, Misova M, Ratheesh
    A, Dos Reis Rodrigues P, Shkarina K, Larsen ISB, Vakhrushev SY, Clausen H, Siekhaus
    DE. 2019. A conserved major facilitator superfamily member orchestrates a subset
    of O-glycosylation to aid macrophage tissue invasion. eLife. 8, e41801.
  mla: Valosková, Katarina, et al. “A Conserved Major Facilitator Superfamily Member
    Orchestrates a Subset of O-Glycosylation to Aid Macrophage Tissue Invasion.” <i>ELife</i>,
    vol. 8, e41801, eLife Sciences Publications, 2019, doi:<a href="https://doi.org/10.7554/elife.41801">10.7554/elife.41801</a>.
  short: K. Valosková, J. Bicher, M. Roblek, S. Emtenani, A. György, M. Misova, A.
    Ratheesh, P. Dos Reis Rodrigues, K. Shkarina, I.S.B. Larsen, S.Y. Vakhrushev,
    H. Clausen, D.E. Siekhaus, ELife 8 (2019).
date_created: 2019-03-28T13:37:45Z
date_published: 2019-03-26T00:00:00Z
date_updated: 2026-04-27T22:30:48Z
day: '26'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.7554/elife.41801
ec_funded: 1
external_id:
  isi:
  - '000462530200001'
file:
- access_level: open_access
  checksum: cc0d1a512559d52e7e7cb0e9b9854b40
  content_type: application/pdf
  creator: dernst
  date_created: 2019-03-28T14:00:41Z
  date_updated: 2020-07-14T12:47:23Z
  file_id: '6188'
  file_name: 2019_eLife_Valoskova.pdf
  file_size: 4496017
  relation: main_file
file_date_updated: 2020-07-14T12:47:23Z
has_accepted_license: '1'
intvolume: '         8'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 253CDE40-B435-11E9-9278-68D0E5697425
  grant_number: '24283'
  name: Examination of the role of a MFS transporter in the migration of Drosophila
    immune cells
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29638
  name: The role of Drosophila TNF alpha in immune cell invasion
- _id: 2536F660-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '334077'
  name: Investigating the role of transporters in invasive migration through junctions
- _id: 25388084-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '329540'
  name: 'Breaking barriers: Investigating the junctional and mechanobiological changes
    underlying the ability of Drosophila immune cells to invade an epithelium'
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
publication: eLife
publication_identifier:
  issn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/new-gene-potentially-involved-in-metastasis-identified/
  record:
  - id: '6530'
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  - id: '8983'
    relation: dissertation_contains
    status: public
  - id: '6546'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation
  to aid macrophage tissue invasion
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 8
year: '2019'
...