[{"article_processing_charge":"Yes (in subscription journal)","date_updated":"2026-03-16T12:55:18Z","_id":"17884","external_id":{"oaworkid":["W4402316284"],"pmid":["39242978"],"isi":["001306564000001"]},"department":[{"_id":"FlSc"},{"_id":"LeSa"}],"pmid":1,"citation":{"apa":"Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., … Schur, F. K. (2025). Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice. <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41594-024-01390-8\">https://doi.org/10.1038/s41594-024-01390-8</a>","ama":"Obr M, Percipalle M, Chernikova D, et al. Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice. <i>Nature Structural &#38; Molecular Biology</i>. 2025;32:268-276. doi:<a href=\"https://doi.org/10.1038/s41594-024-01390-8\">10.1038/s41594-024-01390-8</a>","mla":"Obr, Martin, et al. “Distinct Stabilization of the Human T Cell Leukemia Virus Type 1 Immature Gag Lattice.” <i>Nature Structural &#38; Molecular Biology</i>, vol. 32, Springer Nature, 2025, pp. 268–76, doi:<a href=\"https://doi.org/10.1038/s41594-024-01390-8\">10.1038/s41594-024-01390-8</a>.","ista":"Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley Esteves D, Mansky LM, Dick RA, Schur FK. 2025. Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice. Nature Structural &#38; Molecular Biology. 32, 268–276.","short":"M. Obr, M. Percipalle, D. Chernikova, H. Yang, A. Thader, G. Pinke, D. Porley Esteves, L.M. Mansky, R.A. Dick, F.K. Schur, Nature Structural &#38; Molecular Biology 32 (2025) 268–276.","chicago":"Obr, Martin, Mathias Percipalle, Darya Chernikova, Huixin Yang, Andreas Thader, Gergely Pinke, Darío Porley Esteves, Louis M. Mansky, Robert A. Dick, and Florian KM Schur. “Distinct Stabilization of the Human T Cell Leukemia Virus Type 1 Immature Gag Lattice.” <i>Nature Structural &#38; Molecular Biology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41594-024-01390-8\">https://doi.org/10.1038/s41594-024-01390-8</a>.","ieee":"M. Obr <i>et al.</i>, “Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice,” <i>Nature Structural &#38; Molecular Biology</i>, vol. 32. Springer Nature, pp. 268–276, 2025."},"publication_identifier":{"eissn":["1545-9985"],"issn":["1545-9993"]},"has_accepted_license":"1","OA_type":"hybrid","language":[{"iso":"eng"}],"OA_place":"publisher","publisher":"Springer Nature","publication_status":"published","APC_amount":"12348 EUR","oa_version":"Published Version","volume":32,"date_published":"2025-02-01T00:00:00Z","publication":"Nature Structural & Molecular Biology","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2025-04-23T07:02:33Z","intvolume":"        32","isi":1,"acknowledgement":"This work was funded by the Institute of Science and Technology Austria (ISTA) and the Austrian Science Fund (grant P31445 to F.K.M.S.). Access to high-resolution cryo-ET data acquisition at European Molecular Biology Laboratory (EMBL) Heidelberg was supported through the EMBL cryo-EM platform. We thank V.-V. Hodirnau at ISTA and W. Hagen and F. Weis at EMBL Heidelberg for support in cryo-ET data acquisition. This research was also supported by the scientific service units of ISTA through resources provided by Scientific Computing, the Life Science Facility, and the EM Facility. L.M.M. was supported by National Institutes of Health grants R01 GM151775 and R21 DE032878 and by the University of Minnesota Masonic Cancer Center. D.P. was supported by the DOC doctoral fellowship program of the Austrian Academy of Sciences. R.A.D was supported by the National Institute of Allergy and Infectious Diseases (grant R01AI147890). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Specifically, we also want to thank A. Schlögl for computational support and J. Hansen and V. Vogt for critical comments on the manuscript. We also thank the other members of the Schur lab for helpful discussions and experimental advice.","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"oaworkid":1,"ddc":["570"],"month":"02","title":"Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice","year":"2025","doi":"10.1038/s41594-024-01390-8","project":[{"_id":"26736D6A-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"P31445","name":"Structural conservation and diversity in retroviral capsid"},{"_id":"9B9C98E0-BA93-11EA-9121-9846C619BF3A","grant_number":"25762","name":"Structural characterization of spumavirus capsid assemblies to understand conserved Ortervirales assembly mechanisms"}],"author":[{"last_name":"Obr","first_name":"Martin","orcid":"0000-0003-1756-6564","full_name":"Obr, Martin","id":"4741CA5A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Mathias","last_name":"Percipalle","full_name":"Percipalle, Mathias","id":"4986e21c-eb97-11eb-a6c2-a4ef0b629971"},{"last_name":"Chernikova","first_name":"Darya","id":"7dbaf460-fa9e-11eb-b0ca-bc7c7ff21ad0","full_name":"Chernikova, Darya"},{"first_name":"Huixin","last_name":"Yang","full_name":"Yang, Huixin"},{"id":"3A18A7B8-F248-11E8-B48F-1D18A9856A87","full_name":"Thader, Andreas","first_name":"Andreas","last_name":"Thader"},{"id":"4D5303E6-F248-11E8-B48F-1D18A9856A87","full_name":"Pinke, Gergely","first_name":"Gergely","last_name":"Pinke"},{"first_name":"Dario J","last_name":"Porley","id":"2FD6EA6C-F248-11E8-B48F-1D18A9856A87","full_name":"Porley, Dario J"},{"full_name":"Mansky, Louis M.","first_name":"Louis M.","last_name":"Mansky"},{"full_name":"Dick, Robert A.","last_name":"Dick","first_name":"Robert A."},{"orcid":"0000-0003-4790-8078","first_name":"Florian KM","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","full_name":"Schur, Florian KM"}],"page":"268-276","scopus_import":"1","day":"01","article_type":"original","corr_author":"1","quality_controlled":"1","status":"public","abstract":[{"text":"Human T cell leukemia virus type 1 (HTLV-1) immature particles differ in morphology from other retroviruses, suggesting a distinct way of assembly. Here we report the results of cryo-electron tomography studies of HTLV-1 virus-like particles assembled in vitro, as well as derived from cells. This work shows that HTLV-1 uses a distinct mechanism of Gag–Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature capsid (CA) tubular arrays reveals that the primary stabilizing component in HTLV-1 is the N-terminal domain of CA. Mutagenesis analysis supports this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the C-terminal domain of CA. These results provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus and this helps explain why HTLV-1 particles are morphologically distinct.","lang":"eng"}],"date_created":"2024-09-08T10:29:06Z","oa":1,"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"file":[{"file_id":"19608","checksum":"c641ad94afb28917b20425db676fc3ee","file_size":13724041,"access_level":"open_access","creator":"dernst","date_updated":"2025-04-23T07:02:33Z","relation":"main_file","success":1,"file_name":"2025_NatureStrucBio_Obr.pdf","content_type":"application/pdf","date_created":"2025-04-23T07:02:33Z"}],"type":"journal_article"},{"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"acknowledgement":"This work was supported by a postdoctoral fellowship from the Swedish Society for Medical Research to J.R., a CAPES-STINT joint grant to R.G.G. and L.S.W., a PhD fellowship from Karolinska Institutet (KID) to E.D., a PhD fellowship from Fundação para a Ciência e a Tecnologia and European Social Fund to M.M.S.O., the program of fundamental research (theme 65.1) of the Institute for Biomedical Problems of the Russian Academy of Sciences (IBMP RAS) to A.A.S., S.M.S., V.A.S., O.V.K., D.D.V., K.D.O., M.P.R., and S.A.P., the Tamkeen under the NYU Abu Dhabi Research Institute Award to the NYUAD Center for Genomics and Systems Biology (ADHPG-CGSB) to P.P., the Knut and Alice Wallenberg foundation to C.K., the Swedish National Space Agency to N.V.K. and L.S.W., Swedish Research Council, Gösta Fraenckel Foundation, and Karolinska Institutet to L.S.W.","isi":1,"intvolume":"         9","file_date_updated":"2024-01-16T09:35:28Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Science Advances","date_published":"2023-08-25T00:00:00Z","volume":9,"oa_version":"Published Version","publication_status":"published","publisher":"American Association for the Advancement of Science","language":[{"iso":"eng"}],"has_accepted_license":"1","publication_identifier":{"issn":["2375-2548"]},"citation":{"ieee":"C. J. Gallardo-Dodd <i>et al.</i>, “Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells,” <i>Science Advances</i>, vol. 9, no. 34. American Association for the Advancement of Science, 2023.","chicago":"Gallardo-Dodd, Carlos J., Christian Oertlin, Julien Record, Rômulo G. Galvani, Christian Sommerauer, Nikolai V. Kuznetsov, Evangelos Doukoumopoulos, et al. “Exposure of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation of T Cells.” <i>Science Advances</i>. American Association for the Advancement of Science, 2023. <a href=\"https://doi.org/10.1126/sciadv.adg1610\">https://doi.org/10.1126/sciadv.adg1610</a>.","apa":"Gallardo-Dodd, C. J., Oertlin, C., Record, J., Galvani, R. G., Sommerauer, C., Kuznetsov, N. V., … Westerberg, L. S. (2023). Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.adg1610\">https://doi.org/10.1126/sciadv.adg1610</a>","short":"C.J. Gallardo-Dodd, C. Oertlin, J. Record, R.G. Galvani, C. Sommerauer, N.V. Kuznetsov, E. Doukoumopoulos, L. Ali, M.M.S. Oliveira, C. Seitz, M. Percipalle, T. Nikić, A.A. Sadova, S.M. Shulgina, V.A. Shmarov, O.V. Kutko, D.D. Vlasova, K.D. Orlova, M.P. Rykova, J. Andersson, P. Percipalle, C. Kutter, S.A. Ponomarev, L.S. Westerberg, Science Advances 9 (2023).","ista":"Gallardo-Dodd CJ, Oertlin C, Record J, Galvani RG, Sommerauer C, Kuznetsov NV, Doukoumopoulos E, Ali L, Oliveira MMS, Seitz C, Percipalle M, Nikić T, Sadova AA, Shulgina SM, Shmarov VA, Kutko OV, Vlasova DD, Orlova KD, Rykova MP, Andersson J, Percipalle P, Kutter C, Ponomarev SA, Westerberg LS. 2023. Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. Science Advances. 9(34), adg1610.","mla":"Gallardo-Dodd, Carlos J., et al. “Exposure of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation of T Cells.” <i>Science Advances</i>, vol. 9, no. 34, adg1610, American Association for the Advancement of Science, 2023, doi:<a href=\"https://doi.org/10.1126/sciadv.adg1610\">10.1126/sciadv.adg1610</a>.","ama":"Gallardo-Dodd CJ, Oertlin C, Record J, et al. Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. <i>Science Advances</i>. 2023;9(34). doi:<a href=\"https://doi.org/10.1126/sciadv.adg1610\">10.1126/sciadv.adg1610</a>"},"pmid":1,"department":[{"_id":"FlSc"}],"external_id":{"isi":["001054596800007"],"pmid":["37624890"]},"_id":"14784","date_updated":"2024-09-09T08:03:13Z","article_processing_charge":"Yes","issue":"34","type":"journal_article","keyword":["Multidisciplinary"],"file":[{"relation":"main_file","creator":"dernst","access_level":"open_access","date_updated":"2024-01-16T09:35:28Z","checksum":"b9072e20e2d5d9d34d2c53319bafee41","file_size":1596639,"file_id":"14809","file_name":"2023_ScienceAdvances_GallardoDodd.pdf","date_created":"2024-01-16T09:35:28Z","content_type":"application/pdf","success":1}],"oa":1,"date_created":"2024-01-10T09:48:01Z","abstract":[{"lang":"eng","text":"The next steps of deep space exploration are manned missions to Moon and Mars. For safe space missions for crew members, it is important to understand the impact of space flight on the immune system. We studied the effects of 21 days dry immersion (DI) exposure on the transcriptomes of T cells isolated from blood samples of eight healthy volunteers. Samples were collected 7 days before DI, at day 7, 14, and 21 during DI, and 7 days after DI. RNA sequencing of CD3+T cells revealed transcriptional alterations across all time points, with most changes occurring 14 days after DI exposure. At day 21, T cells showed evidence of adaptation with a transcriptional profile resembling that of 7 days before DI. At 7 days after DI, T cells again changed their transcriptional profile. These data suggest that T cells adapt by rewiring their transcriptomes in response to simulated weightlessness and that remodeling cues persist when reexposed to normal gravity."}],"status":"public","quality_controlled":"1","article_type":"original","article_number":"adg1610","day":"25","author":[{"full_name":"Gallardo-Dodd, Carlos J.","first_name":"Carlos J.","last_name":"Gallardo-Dodd"},{"last_name":"Oertlin","first_name":"Christian","full_name":"Oertlin, Christian"},{"full_name":"Record, Julien","last_name":"Record","first_name":"Julien"},{"last_name":"Galvani","first_name":"Rômulo G.","full_name":"Galvani, Rômulo G."},{"full_name":"Sommerauer, Christian","last_name":"Sommerauer","first_name":"Christian"},{"full_name":"Kuznetsov, Nikolai V.","last_name":"Kuznetsov","first_name":"Nikolai V."},{"full_name":"Doukoumopoulos, Evangelos","first_name":"Evangelos","last_name":"Doukoumopoulos"},{"full_name":"Ali, Liaqat","first_name":"Liaqat","last_name":"Ali"},{"full_name":"Oliveira, Mariana M. S.","first_name":"Mariana M. S.","last_name":"Oliveira"},{"last_name":"Seitz","first_name":"Christina","full_name":"Seitz, Christina"},{"full_name":"Percipalle, Mathias","id":"4986e21c-eb97-11eb-a6c2-a4ef0b629971","last_name":"Percipalle","first_name":"Mathias"},{"first_name":"Tijana","last_name":"Nikić","full_name":"Nikić, Tijana"},{"full_name":"Sadova, Anastasia A.","last_name":"Sadova","first_name":"Anastasia A."},{"last_name":"Shulgina","first_name":"Sofia M.","full_name":"Shulgina, Sofia M."},{"full_name":"Shmarov, Vjacheslav A.","first_name":"Vjacheslav A.","last_name":"Shmarov"},{"last_name":"Kutko","first_name":"Olga V.","full_name":"Kutko, Olga V."},{"full_name":"Vlasova, Daria D.","last_name":"Vlasova","first_name":"Daria D."},{"full_name":"Orlova, Kseniya D.","first_name":"Kseniya D.","last_name":"Orlova"},{"last_name":"Rykova","first_name":"Marina P.","full_name":"Rykova, Marina P."},{"last_name":"Andersson","first_name":"John","full_name":"Andersson, John"},{"first_name":"Piergiorgio","last_name":"Percipalle","full_name":"Percipalle, Piergiorgio"},{"full_name":"Kutter, Claudia","last_name":"Kutter","first_name":"Claudia"},{"full_name":"Ponomarev, Sergey A.","first_name":"Sergey A.","last_name":"Ponomarev"},{"full_name":"Westerberg, Lisa S.","first_name":"Lisa S.","last_name":"Westerberg"}],"year":"2023","doi":"10.1126/sciadv.adg1610","title":"Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells","month":"08","ddc":["570"]}]