---
APC_amount: 12348 EUR
OA_place: publisher
OA_type: hybrid
_id: '17884'
abstract:
- lang: eng
  text: Human T cell leukemia virus type 1 (HTLV-1) immature particles differ in morphology
    from other retroviruses, suggesting a distinct way of assembly. Here we report
    the results of cryo-electron tomography studies of HTLV-1 virus-like particles
    assembled in vitro, as well as derived from cells. This work shows that HTLV-1
    uses a distinct mechanism of Gag–Gag interactions to form the immature viral lattice.
    Analysis of high-resolution structural information from immature capsid (CA) tubular
    arrays reveals that the primary stabilizing component in HTLV-1 is the N-terminal
    domain of CA. Mutagenesis analysis supports this observation. This distinguishes
    HTLV-1 from other retroviruses, in which the stabilization is provided primarily
    by the C-terminal domain of CA. These results provide structural details of the
    quaternary arrangement of Gag for an immature deltaretrovirus and this helps explain
    why HTLV-1 particles are morphologically distinct.
acknowledged_ssus:
- _id: ScienComp
- _id: LifeSc
- _id: EM-Fac
acknowledgement: This work was funded by the Institute of Science and Technology Austria
  (ISTA) and the Austrian Science Fund (grant P31445 to F.K.M.S.). Access to high-resolution
  cryo-ET data acquisition at European Molecular Biology Laboratory (EMBL) Heidelberg
  was supported through the EMBL cryo-EM platform. We thank V.-V. Hodirnau at ISTA
  and W. Hagen and F. Weis at EMBL Heidelberg for support in cryo-ET data acquisition.
  This research was also supported by the scientific service units of ISTA through
  resources provided by Scientific Computing, the Life Science Facility, and the EM
  Facility. L.M.M. was supported by National Institutes of Health grants R01 GM151775
  and R21 DE032878 and by the University of Minnesota Masonic Cancer Center. D.P.
  was supported by the DOC doctoral fellowship program of the Austrian Academy of
  Sciences. R.A.D was supported by the National Institute of Allergy and Infectious
  Diseases (grant R01AI147890). The funders had no role in study design, data collection
  and analysis, decision to publish or preparation of the manuscript. Specifically,
  we also want to thank A. Schlögl for computational support and J. Hansen and V.
  Vogt for critical comments on the manuscript. We also thank the other members of
  the Schur lab for helpful discussions and experimental advice.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Martin
  full_name: Obr, Martin
  id: 4741CA5A-F248-11E8-B48F-1D18A9856A87
  last_name: Obr
  orcid: 0000-0003-1756-6564
- first_name: Mathias
  full_name: Percipalle, Mathias
  id: 4986e21c-eb97-11eb-a6c2-a4ef0b629971
  last_name: Percipalle
- first_name: Darya
  full_name: Chernikova, Darya
  id: 7dbaf460-fa9e-11eb-b0ca-bc7c7ff21ad0
  last_name: Chernikova
- first_name: Huixin
  full_name: Yang, Huixin
  last_name: Yang
- first_name: Andreas
  full_name: Thader, Andreas
  id: 3A18A7B8-F248-11E8-B48F-1D18A9856A87
  last_name: Thader
- first_name: Gergely
  full_name: Pinke, Gergely
  id: 4D5303E6-F248-11E8-B48F-1D18A9856A87
  last_name: Pinke
- first_name: Dario J
  full_name: Porley, Dario J
  id: 2FD6EA6C-F248-11E8-B48F-1D18A9856A87
  last_name: Porley
- first_name: Louis M.
  full_name: Mansky, Louis M.
  last_name: Mansky
- first_name: Robert A.
  full_name: Dick, Robert A.
  last_name: Dick
- first_name: Florian KM
  full_name: Schur, Florian KM
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
citation:
  ama: Obr M, Percipalle M, Chernikova D, et al. Distinct stabilization of the human
    T cell leukemia virus type 1 immature Gag lattice. <i>Nature Structural &#38;
    Molecular Biology</i>. 2025;32:268-276. doi:<a href="https://doi.org/10.1038/s41594-024-01390-8">10.1038/s41594-024-01390-8</a>
  apa: Obr, M., Percipalle, M., Chernikova, D., Yang, H., Thader, A., Pinke, G., …
    Schur, F. K. (2025). Distinct stabilization of the human T cell leukemia virus
    type 1 immature Gag lattice. <i>Nature Structural &#38; Molecular Biology</i>.
    Springer Nature. <a href="https://doi.org/10.1038/s41594-024-01390-8">https://doi.org/10.1038/s41594-024-01390-8</a>
  chicago: Obr, Martin, Mathias Percipalle, Darya Chernikova, Huixin Yang, Andreas
    Thader, Gergely Pinke, Darío Porley Esteves, Louis M. Mansky, Robert A. Dick,
    and Florian KM Schur. “Distinct Stabilization of the Human T Cell Leukemia Virus
    Type 1 Immature Gag Lattice.” <i>Nature Structural &#38; Molecular Biology</i>.
    Springer Nature, 2025. <a href="https://doi.org/10.1038/s41594-024-01390-8">https://doi.org/10.1038/s41594-024-01390-8</a>.
  ieee: M. Obr <i>et al.</i>, “Distinct stabilization of the human T cell leukemia
    virus type 1 immature Gag lattice,” <i>Nature Structural &#38; Molecular Biology</i>,
    vol. 32. Springer Nature, pp. 268–276, 2025.
  ista: Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley Esteves
    D, Mansky LM, Dick RA, Schur FK. 2025. Distinct stabilization of the human T cell
    leukemia virus type 1 immature Gag lattice. Nature Structural &#38; Molecular
    Biology. 32, 268–276.
  mla: Obr, Martin, et al. “Distinct Stabilization of the Human T Cell Leukemia Virus
    Type 1 Immature Gag Lattice.” <i>Nature Structural &#38; Molecular Biology</i>,
    vol. 32, Springer Nature, 2025, pp. 268–76, doi:<a href="https://doi.org/10.1038/s41594-024-01390-8">10.1038/s41594-024-01390-8</a>.
  short: M. Obr, M. Percipalle, D. Chernikova, H. Yang, A. Thader, G. Pinke, D. Porley
    Esteves, L.M. Mansky, R.A. Dick, F.K. Schur, Nature Structural &#38; Molecular
    Biology 32 (2025) 268–276.
corr_author: '1'
date_created: 2024-09-08T10:29:06Z
date_published: 2025-02-01T00:00:00Z
date_updated: 2026-03-16T12:55:18Z
day: '01'
ddc:
- '570'
department:
- _id: FlSc
- _id: LeSa
doi: 10.1038/s41594-024-01390-8
external_id:
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  - '39242978'
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fulldoi: https://doi.org/10.1038/s41594-024-01390-8
has_accepted_license: '1'
intvolume: '        32'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
oaworkid: 1
page: 268-276
pmid: 1
project:
- _id: 26736D6A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P31445
  name: Structural conservation and diversity in retroviral capsid
- _id: 9B9C98E0-BA93-11EA-9121-9846C619BF3A
  grant_number: '25762'
  name: Structural characterization of spumavirus capsid assemblies to understand
    conserved Ortervirales assembly mechanisms
publication: Nature Structural & Molecular Biology
publication_identifier:
  eissn:
  - 1545-9985
  issn:
  - 1545-9993
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Distinct stabilization of the human T cell leukemia virus type 1 immature Gag
  lattice
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2025'
...
---
_id: '14784'
abstract:
- lang: eng
  text: The next steps of deep space exploration are manned missions to Moon and Mars.
    For safe space missions for crew members, it is important to understand the impact
    of space flight on the immune system. We studied the effects of 21 days dry immersion
    (DI) exposure on the transcriptomes of T cells isolated from blood samples of
    eight healthy volunteers. Samples were collected 7 days before DI, at day 7, 14,
    and 21 during DI, and 7 days after DI. RNA sequencing of CD3+T cells revealed
    transcriptional alterations across all time points, with most changes occurring
    14 days after DI exposure. At day 21, T cells showed evidence of adaptation with
    a transcriptional profile resembling that of 7 days before DI. At 7 days after
    DI, T cells again changed their transcriptional profile. These data suggest that
    T cells adapt by rewiring their transcriptomes in response to simulated weightlessness
    and that remodeling cues persist when reexposed to normal gravity.
acknowledgement: This work was supported by a postdoctoral fellowship from the Swedish
  Society for Medical Research to J.R., a CAPES-STINT joint grant to R.G.G. and L.S.W.,
  a PhD fellowship from Karolinska Institutet (KID) to E.D., a PhD fellowship from
  Fundação para a Ciência e a Tecnologia and European Social Fund to M.M.S.O., the
  program of fundamental research (theme 65.1) of the Institute for Biomedical Problems
  of the Russian Academy of Sciences (IBMP RAS) to A.A.S., S.M.S., V.A.S., O.V.K.,
  D.D.V., K.D.O., M.P.R., and S.A.P., the Tamkeen under the NYU Abu Dhabi Research
  Institute Award to the NYUAD Center for Genomics and Systems Biology (ADHPG-CGSB)
  to P.P., the Knut and Alice Wallenberg foundation to C.K., the Swedish National
  Space Agency to N.V.K. and L.S.W., Swedish Research Council, Gösta Fraenckel Foundation,
  and Karolinska Institutet to L.S.W.
article_number: adg1610
article_processing_charge: Yes
article_type: original
author:
- first_name: Carlos J.
  full_name: Gallardo-Dodd, Carlos J.
  last_name: Gallardo-Dodd
- first_name: Christian
  full_name: Oertlin, Christian
  last_name: Oertlin
- first_name: Julien
  full_name: Record, Julien
  last_name: Record
- first_name: Rômulo G.
  full_name: Galvani, Rômulo G.
  last_name: Galvani
- first_name: Christian
  full_name: Sommerauer, Christian
  last_name: Sommerauer
- first_name: Nikolai V.
  full_name: Kuznetsov, Nikolai V.
  last_name: Kuznetsov
- first_name: Evangelos
  full_name: Doukoumopoulos, Evangelos
  last_name: Doukoumopoulos
- first_name: Liaqat
  full_name: Ali, Liaqat
  last_name: Ali
- first_name: Mariana M. S.
  full_name: Oliveira, Mariana M. S.
  last_name: Oliveira
- first_name: Christina
  full_name: Seitz, Christina
  last_name: Seitz
- first_name: Mathias
  full_name: Percipalle, Mathias
  id: 4986e21c-eb97-11eb-a6c2-a4ef0b629971
  last_name: Percipalle
- first_name: Tijana
  full_name: Nikić, Tijana
  last_name: Nikić
- first_name: Anastasia A.
  full_name: Sadova, Anastasia A.
  last_name: Sadova
- first_name: Sofia M.
  full_name: Shulgina, Sofia M.
  last_name: Shulgina
- first_name: Vjacheslav A.
  full_name: Shmarov, Vjacheslav A.
  last_name: Shmarov
- first_name: Olga V.
  full_name: Kutko, Olga V.
  last_name: Kutko
- first_name: Daria D.
  full_name: Vlasova, Daria D.
  last_name: Vlasova
- first_name: Kseniya D.
  full_name: Orlova, Kseniya D.
  last_name: Orlova
- first_name: Marina P.
  full_name: Rykova, Marina P.
  last_name: Rykova
- first_name: John
  full_name: Andersson, John
  last_name: Andersson
- first_name: Piergiorgio
  full_name: Percipalle, Piergiorgio
  last_name: Percipalle
- first_name: Claudia
  full_name: Kutter, Claudia
  last_name: Kutter
- first_name: Sergey A.
  full_name: Ponomarev, Sergey A.
  last_name: Ponomarev
- first_name: Lisa S.
  full_name: Westerberg, Lisa S.
  last_name: Westerberg
citation:
  ama: Gallardo-Dodd CJ, Oertlin C, Record J, et al. Exposure of volunteers to microgravity
    by dry immersion bed over 21 days results in gene expression changes and adaptation
    of T cells. <i>Science Advances</i>. 2023;9(34). doi:<a href="https://doi.org/10.1126/sciadv.adg1610">10.1126/sciadv.adg1610</a>
  apa: Gallardo-Dodd, C. J., Oertlin, C., Record, J., Galvani, R. G., Sommerauer,
    C., Kuznetsov, N. V., … Westerberg, L. S. (2023). Exposure of volunteers to microgravity
    by dry immersion bed over 21 days results in gene expression changes and adaptation
    of T cells. <i>Science Advances</i>. American Association for the Advancement
    of Science. <a href="https://doi.org/10.1126/sciadv.adg1610">https://doi.org/10.1126/sciadv.adg1610</a>
  chicago: Gallardo-Dodd, Carlos J., Christian Oertlin, Julien Record, Rômulo G. Galvani,
    Christian Sommerauer, Nikolai V. Kuznetsov, Evangelos Doukoumopoulos, et al. “Exposure
    of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene
    Expression Changes and Adaptation of T Cells.” <i>Science Advances</i>. American
    Association for the Advancement of Science, 2023. <a href="https://doi.org/10.1126/sciadv.adg1610">https://doi.org/10.1126/sciadv.adg1610</a>.
  ieee: C. J. Gallardo-Dodd <i>et al.</i>, “Exposure of volunteers to microgravity
    by dry immersion bed over 21 days results in gene expression changes and adaptation
    of T cells,” <i>Science Advances</i>, vol. 9, no. 34. American Association for
    the Advancement of Science, 2023.
  ista: Gallardo-Dodd CJ, Oertlin C, Record J, Galvani RG, Sommerauer C, Kuznetsov
    NV, Doukoumopoulos E, Ali L, Oliveira MMS, Seitz C, Percipalle M, Nikić T, Sadova
    AA, Shulgina SM, Shmarov VA, Kutko OV, Vlasova DD, Orlova KD, Rykova MP, Andersson
    J, Percipalle P, Kutter C, Ponomarev SA, Westerberg LS. 2023. Exposure of volunteers
    to microgravity by dry immersion bed over 21 days results in gene expression changes
    and adaptation of T cells. Science Advances. 9(34), adg1610.
  mla: Gallardo-Dodd, Carlos J., et al. “Exposure of Volunteers to Microgravity by
    Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation
    of T Cells.” <i>Science Advances</i>, vol. 9, no. 34, adg1610, American Association
    for the Advancement of Science, 2023, doi:<a href="https://doi.org/10.1126/sciadv.adg1610">10.1126/sciadv.adg1610</a>.
  short: C.J. Gallardo-Dodd, C. Oertlin, J. Record, R.G. Galvani, C. Sommerauer, N.V.
    Kuznetsov, E. Doukoumopoulos, L. Ali, M.M.S. Oliveira, C. Seitz, M. Percipalle,
    T. Nikić, A.A. Sadova, S.M. Shulgina, V.A. Shmarov, O.V. Kutko, D.D. Vlasova,
    K.D. Orlova, M.P. Rykova, J. Andersson, P. Percipalle, C. Kutter, S.A. Ponomarev,
    L.S. Westerberg, Science Advances 9 (2023).
date_created: 2024-01-10T09:48:01Z
date_published: 2023-08-25T00:00:00Z
date_updated: 2024-09-09T08:03:13Z
day: '25'
ddc:
- '570'
department:
- _id: FlSc
doi: 10.1126/sciadv.adg1610
external_id:
  isi:
  - '001054596800007'
  pmid:
  - '37624890'
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  date_created: 2024-01-16T09:35:28Z
  date_updated: 2024-01-16T09:35:28Z
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fulldoi: https://doi.org/10.1126/sciadv.adg1610
has_accepted_license: '1'
intvolume: '         9'
isi: 1
issue: '34'
keyword:
- Multidisciplinary
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
  issn:
  - 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
status: public
title: Exposure of volunteers to microgravity by dry immersion bed over 21 days results
  in gene expression changes and adaptation of T cells
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2023'
...