---
_id: '9245'
abstract:
- lang: eng
  text: Tissue morphogenesis is driven by mechanical forces triggering cell movements
    and shape changes. Quantitatively measuring tension within tissues is of great
    importance for understanding the role of mechanical signals acting on the cell
    and tissue level during morphogenesis. Here we introduce laser ablation as a useful
    tool to probe tissue tension within the granulosa layer, an epithelial monolayer
    of somatic cells that surround the zebrafish female gamete during folliculogenesis.
    We describe in detail how to isolate follicles, mount samples, perform laser surgery,
    and analyze the data.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank Prof. Masazumi Tada and Roland Dosch for providing transgenic
  zebrafish lines, the Heisenberg lab for technical assistance and feedback on the
  manuscript, and the Bioimaging and Fish facilities of IST Austria for continuous
  support. This work was funded by an ERC advanced grant (MECSPEC to C.-P.H.).
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Xia P, Heisenberg C-PJ. Quantifying tissue tension in the granulosa layer
    after laser surgery. In: Dosch R, ed. <i>Germline Development in the Zebrafish</i>.
    Vol 2218. Humana; 2021:117-128. doi:<a href="https://doi.org/10.1007/978-1-0716-0970-5_10">10.1007/978-1-0716-0970-5_10</a>'
  apa: Xia, P., &#38; Heisenberg, C.-P. J. (2021). Quantifying tissue tension in the
    granulosa layer after laser surgery. In R. Dosch (Ed.), <i>Germline Development
    in the Zebrafish</i> (Vol. 2218, pp. 117–128). Humana. <a href="https://doi.org/10.1007/978-1-0716-0970-5_10">https://doi.org/10.1007/978-1-0716-0970-5_10</a>
  chicago: Xia, Peng, and Carl-Philipp J Heisenberg. “Quantifying Tissue Tension in
    the Granulosa Layer after Laser Surgery.” In <i>Germline Development in the Zebrafish</i>,
    edited by Roland Dosch, 2218:117–28. Humana, 2021. <a href="https://doi.org/10.1007/978-1-0716-0970-5_10">https://doi.org/10.1007/978-1-0716-0970-5_10</a>.
  ieee: P. Xia and C.-P. J. Heisenberg, “Quantifying tissue tension in the granulosa
    layer after laser surgery,” in <i>Germline Development in the Zebrafish</i>, vol.
    2218, R. Dosch, Ed. Humana, 2021, pp. 117–128.
  ista: 'Xia P, Heisenberg C-PJ. 2021.Quantifying tissue tension in the granulosa
    layer after laser surgery. In: Germline Development in the Zebrafish. Methods
    in Molecular Biology, vol. 2218, 117–128.'
  mla: Xia, Peng, and Carl-Philipp J. Heisenberg. “Quantifying Tissue Tension in the
    Granulosa Layer after Laser Surgery.” <i>Germline Development in the Zebrafish</i>,
    edited by Roland Dosch, vol. 2218, Humana, 2021, pp. 117–28, doi:<a href="https://doi.org/10.1007/978-1-0716-0970-5_10">10.1007/978-1-0716-0970-5_10</a>.
  short: P. Xia, C.-P.J. Heisenberg, in:, R. Dosch (Ed.), Germline Development in
    the Zebrafish, Humana, 2021, pp. 117–128.
corr_author: '1'
date_created: 2021-03-14T23:01:34Z
date_published: 2021-02-20T00:00:00Z
date_updated: 2025-04-14T07:46:58Z
day: '20'
department:
- _id: CaHe
doi: 10.1007/978-1-0716-0970-5_10
ec_funded: 1
editor:
- first_name: Roland
  full_name: Dosch, Roland
  last_name: Dosch
external_id:
  pmid:
  - '33606227'
intvolume: '      2218'
keyword:
- Tissue tension
- Morphogenesis
- Laser ablation
- Zebrafish folliculogenesis
- Granulosa cells
language:
- iso: eng
month: '02'
oa_version: None
page: 117-128
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Germline Development in the Zebrafish
publication_identifier:
  eisbn:
  - 978-1-0716-0970-5
  eissn:
  - 1940-6029
  isbn:
  - 978-1-0716-0969-9
  issn:
  - 1064-3745
publication_status: published
publisher: Humana
quality_controlled: '1'
scopus_import: '1'
status: public
title: Quantifying tissue tension in the granulosa layer after laser surgery
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2218
year: '2021'
...
---
_id: '8680'
abstract:
- lang: eng
  text: Animal development entails the organization of specific cell types in space
    and time, and spatial patterns must form in a robust manner. In the zebrafish
    spinal cord, neural progenitors form stereotypic patterns despite noisy morphogen
    signaling and large-scale cellular rearrangements during morphogenesis and growth.
    By directly measuring adhesion forces and preferences for three types of endogenous
    neural progenitors, we provide evidence for the differential adhesion model in
    which differences in intercellular adhesion mediate cell sorting. Cell type–specific
    combinatorial expression of different classes of cadherins (N-cadherin, cadherin
    11, and protocadherin 19) results in homotypic preference ex vivo and patterning
    robustness in vivo. Furthermore, the differential adhesion code is regulated by
    the sonic hedgehog morphogen gradient. We propose that robust patterning during
    tissue morphogenesis results from interplay between adhesion-based self-organization
    and morphogen-directed patterning.
acknowledgement: "We thank the members of the Megason and Heisenberg labs for critical
  discussions of and technical assistance during the work and B. Appel, S. Holley,
  J. Jontes, and D. Gilmour for transgenic fish. This work is supported by the Damon
  Runyon Cancer Foundation, a NICHD K99 fellowship (1K99HD092623), a Travelling Fellowship
  of the Company of Biologists, a Collaborative Research grant from the Burroughs
  Wellcome Foundation (T.Y.-C.T.), NIH grant  01GM107733 (T.Y.-C.T. and S.G.M.), NIH
  grant R01NS102322 (T.C.-C. and H.K.), and an ERC advanced grant\r\n(MECSPEC) (C.-P.H.)."
article_processing_charge: No
article_type: original
author:
- first_name: Tony Y.-C.
  full_name: Tsai, Tony Y.-C.
  last_name: Tsai
- first_name: Mateusz K
  full_name: Sikora, Mateusz K
  id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
  last_name: Sikora
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Tugba
  full_name: Colak-Champollion, Tugba
  last_name: Colak-Champollion
- first_name: Holger
  full_name: Knaut, Holger
  last_name: Knaut
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Sean G.
  full_name: Megason, Sean G.
  last_name: Megason
citation:
  ama: Tsai TY-C, Sikora MK, Xia P, et al. An adhesion code ensures robust pattern
    formation during tissue morphogenesis. <i>Science</i>. 2020;370(6512):113-116.
    doi:<a href="https://doi.org/10.1126/science.aba6637">10.1126/science.aba6637</a>
  apa: Tsai, T. Y.-C., Sikora, M. K., Xia, P., Colak-Champollion, T., Knaut, H., Heisenberg,
    C.-P. J., &#38; Megason, S. G. (2020). An adhesion code ensures robust pattern
    formation during tissue morphogenesis. <i>Science</i>. American Association for
    the Advancement of Science. <a href="https://doi.org/10.1126/science.aba6637">https://doi.org/10.1126/science.aba6637</a>
  chicago: Tsai, Tony Y.-C., Mateusz K Sikora, Peng Xia, Tugba Colak-Champollion,
    Holger Knaut, Carl-Philipp J Heisenberg, and Sean G. Megason. “An Adhesion Code
    Ensures Robust Pattern Formation during Tissue Morphogenesis.” <i>Science</i>.
    American Association for the Advancement of Science, 2020. <a href="https://doi.org/10.1126/science.aba6637">https://doi.org/10.1126/science.aba6637</a>.
  ieee: T. Y.-C. Tsai <i>et al.</i>, “An adhesion code ensures robust pattern formation
    during tissue morphogenesis,” <i>Science</i>, vol. 370, no. 6512. American Association
    for the Advancement of Science, pp. 113–116, 2020.
  ista: Tsai TY-C, Sikora MK, Xia P, Colak-Champollion T, Knaut H, Heisenberg C-PJ,
    Megason SG. 2020. An adhesion code ensures robust pattern formation during tissue
    morphogenesis. Science. 370(6512), 113–116.
  mla: Tsai, Tony Y. C., et al. “An Adhesion Code Ensures Robust Pattern Formation
    during Tissue Morphogenesis.” <i>Science</i>, vol. 370, no. 6512, American Association
    for the Advancement of Science, 2020, pp. 113–16, doi:<a href="https://doi.org/10.1126/science.aba6637">10.1126/science.aba6637</a>.
  short: T.Y.-C. Tsai, M.K. Sikora, P. Xia, T. Colak-Champollion, H. Knaut, C.-P.J.
    Heisenberg, S.G. Megason, Science 370 (2020) 113–116.
date_created: 2020-10-19T14:09:38Z
date_published: 2020-10-02T00:00:00Z
date_updated: 2025-06-12T07:00:41Z
day: '02'
department:
- _id: CaHe
doi: 10.1126/science.aba6637
ec_funded: 1
external_id:
  isi:
  - '000579169000053'
  pmid:
  - '33004519'
intvolume: '       370'
isi: 1
issue: '6512'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.biorxiv.org/content/10.1101/803635v1
month: '10'
oa: 1
oa_version: Preprint
page: 113-116
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Science
publication_identifier:
  eissn:
  - 1095-9203
  issn:
  - 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/sticking-together/
scopus_import: '1'
status: public
title: An adhesion code ensures robust pattern formation during tissue morphogenesis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 370
year: '2020'
...
---
_id: '6087'
abstract:
- lang: eng
  text: Cell fate specification by lateral inhibition typically involves contact signaling
    through the Delta-Notch signaling pathway. However, whether this is the only signaling
    mode mediating lateral inhibition remains unclear. Here we show that in zebrafish
    oogenesis, a group of cells within the granulosa cell layer at the oocyte animal
    pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei.
    One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly
    high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically
    compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly,
    relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear
    TAZ accumulation in neighboring cells, eventually leading to MPC re-specification
    from these cells. Conversely, MPC specification is defective in taz−/− follicles.
    These findings uncover a novel mode of lateral inhibition in cell fate specification
    based on mechanical signals controlling TAZ activity.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We thank Roland Dosch, Makoto Furutani-Seiki, Brian Link, Mary Mullins,
  and Masazumi Tada for providing transgenic and/or mutant zebrafish lines; Alexandra
  Schauer, Shayan Shami-Pour, and the rest of the Heisenberg lab for technical assistance
  and feedback on the manuscript; and the Bioimaging, Electron Microscopy, and Zebrafish
  facilities of IST Austria for continuous support. This work was supported by an
  ERC advanced grant ( MECSPEC to C.-P.H.).
article_processing_charge: No
article_type: original
author:
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Daniel J
  full_name: Gütl, Daniel J
  id: 381929CE-F248-11E8-B48F-1D18A9856A87
  last_name: Gütl
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. Lateral inhibition in cell specification
    mediated by mechanical signals modulating TAZ activity. <i>Cell</i>. 2019;176(6):1379-1392.e14.
    doi:<a href="https://doi.org/10.1016/j.cell.2019.01.019">10.1016/j.cell.2019.01.019</a>
  apa: Xia, P., Gütl, D. J., Zheden, V., &#38; Heisenberg, C.-P. J. (2019). Lateral
    inhibition in cell specification mediated by mechanical signals modulating TAZ
    activity. <i>Cell</i>. Elsevier. <a href="https://doi.org/10.1016/j.cell.2019.01.019">https://doi.org/10.1016/j.cell.2019.01.019</a>
  chicago: Xia, Peng, Daniel J Gütl, Vanessa Zheden, and Carl-Philipp J Heisenberg.
    “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating
    TAZ Activity.” <i>Cell</i>. Elsevier, 2019. <a href="https://doi.org/10.1016/j.cell.2019.01.019">https://doi.org/10.1016/j.cell.2019.01.019</a>.
  ieee: P. Xia, D. J. Gütl, V. Zheden, and C.-P. J. Heisenberg, “Lateral inhibition
    in cell specification mediated by mechanical signals modulating TAZ activity,”
    <i>Cell</i>, vol. 176, no. 6. Elsevier, p. 1379–1392.e14, 2019.
  ista: Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. 2019. Lateral inhibition in cell
    specification mediated by mechanical signals modulating TAZ activity. Cell. 176(6),
    1379–1392.e14.
  mla: Xia, Peng, et al. “Lateral Inhibition in Cell Specification Mediated by Mechanical
    Signals Modulating TAZ Activity.” <i>Cell</i>, vol. 176, no. 6, Elsevier, 2019,
    p. 1379–1392.e14, doi:<a href="https://doi.org/10.1016/j.cell.2019.01.019">10.1016/j.cell.2019.01.019</a>.
  short: P. Xia, D.J. Gütl, V. Zheden, C.-P.J. Heisenberg, Cell 176 (2019) 1379–1392.e14.
date_created: 2019-03-10T22:59:19Z
date_published: 2019-03-07T00:00:00Z
date_updated: 2025-04-14T07:46:59Z
day: '07'
department:
- _id: CaHe
- _id: EM-Fac
doi: 10.1016/j.cell.2019.01.019
ec_funded: 1
external_id:
  isi:
  - '000460509600013'
  pmid:
  - '30773315'
intvolume: '       176'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.cell.2019.01.019
month: '03'
oa: 1
oa_version: Published Version
page: 1379-1392.e14
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/in-zebrafish-eggs-most-rapidly-growing-cell-inhibits-its-neighbours-through-mechanical-signals/
scopus_import: '1'
status: public
title: Lateral inhibition in cell specification mediated by mechanical signals modulating
  TAZ activity
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 176
year: '2019'
...
---
_id: '6899'
abstract:
- lang: eng
  text: Intra-organ communication guides morphogenetic processes that are essential
    for an organ to carry out complex physiological functions. In the heart, the growth
    of the myocardium is tightly coupled to that of the endocardium, a specialized
    endothelial tissue that lines its interior. Several molecular pathways have been
    implicated in the communication between these tissues including secreted factors,
    components of the extracellular matrix, or proteins involved in cell-cell communication.
    Yet, it is unknown how the growth of the endocardium is coordinated with that
    of the myocardium. Here, we show that an increased expansion of the myocardial
    atrial chamber volume generates higher junctional forces within endocardial cells.
    This leads to biomechanical signaling involving VE-cadherin, triggering nuclear
    localization of the Hippo pathway transcriptional regulator Yap1 and endocardial
    proliferation. Our work suggests that the growth of the endocardium results from
    myocardial chamber volume expansion and ends when the tension on the tissue is
    relaxed.
article_processing_charge: No
author:
- first_name: Dorothee
  full_name: Bornhorst, Dorothee
  last_name: Bornhorst
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Hiroyuki
  full_name: Nakajima, Hiroyuki
  last_name: Nakajima
- first_name: Chaitanya
  full_name: Dingare, Chaitanya
  last_name: Dingare
- first_name: Wiebke
  full_name: Herzog, Wiebke
  last_name: Herzog
- first_name: Virginie
  full_name: Lecaudey, Virginie
  last_name: Lecaudey
- first_name: Naoki
  full_name: Mochizuki, Naoki
  last_name: Mochizuki
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Deborah
  full_name: Yelon, Deborah
  last_name: Yelon
- first_name: Salim
  full_name: Abdelilah-Seyfried, Salim
  last_name: Abdelilah-Seyfried
citation:
  ama: Bornhorst D, Xia P, Nakajima H, et al. Biomechanical signaling within the developing
    zebrafish heart attunes endocardial growth to myocardial chamber dimensions. <i>Nature
    communications</i>. 2019;10(1):4113. doi:<a href="https://doi.org/10.1038/s41467-019-12068-x">10.1038/s41467-019-12068-x</a>
  apa: Bornhorst, D., Xia, P., Nakajima, H., Dingare, C., Herzog, W., Lecaudey, V.,
    … Abdelilah-Seyfried, S. (2019). Biomechanical signaling within the developing
    zebrafish heart attunes endocardial growth to myocardial chamber dimensions. <i>Nature
    Communications</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/s41467-019-12068-x">https://doi.org/10.1038/s41467-019-12068-x</a>
  chicago: Bornhorst, Dorothee, Peng Xia, Hiroyuki Nakajima, Chaitanya Dingare, Wiebke
    Herzog, Virginie Lecaudey, Naoki Mochizuki, Carl-Philipp J Heisenberg, Deborah
    Yelon, and Salim Abdelilah-Seyfried. “Biomechanical Signaling within the Developing
    Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.”
    <i>Nature Communications</i>. Nature Publishing Group, 2019. <a href="https://doi.org/10.1038/s41467-019-12068-x">https://doi.org/10.1038/s41467-019-12068-x</a>.
  ieee: D. Bornhorst <i>et al.</i>, “Biomechanical signaling within the developing
    zebrafish heart attunes endocardial growth to myocardial chamber dimensions,”
    <i>Nature communications</i>, vol. 10, no. 1. Nature Publishing Group, p. 4113,
    2019.
  ista: Bornhorst D, Xia P, Nakajima H, Dingare C, Herzog W, Lecaudey V, Mochizuki
    N, Heisenberg C-PJ, Yelon D, Abdelilah-Seyfried S. 2019. Biomechanical signaling
    within the developing zebrafish heart attunes endocardial growth to myocardial
    chamber dimensions. Nature communications. 10(1), 4113.
  mla: Bornhorst, Dorothee, et al. “Biomechanical Signaling within the Developing
    Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.”
    <i>Nature Communications</i>, vol. 10, no. 1, Nature Publishing Group, 2019, p.
    4113, doi:<a href="https://doi.org/10.1038/s41467-019-12068-x">10.1038/s41467-019-12068-x</a>.
  short: D. Bornhorst, P. Xia, H. Nakajima, C. Dingare, W. Herzog, V. Lecaudey, N.
    Mochizuki, C.-P.J. Heisenberg, D. Yelon, S. Abdelilah-Seyfried, Nature Communications
    10 (2019) 4113.
date_created: 2019-09-22T22:00:37Z
date_published: 2019-09-11T00:00:00Z
date_updated: 2026-04-03T09:40:44Z
day: '11'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1038/s41467-019-12068-x
external_id:
  isi:
  - '000485216800009'
  pmid:
  - '31511517'
file:
- access_level: open_access
  checksum: 62c2512712e16d27c1797d318d14ba9f
  content_type: application/pdf
  creator: kschuh
  date_created: 2019-10-01T11:18:50Z
  date_updated: 2020-07-14T12:47:44Z
  file_id: '6926'
  file_name: 2019_Nature_Bornhorst.pdf
  file_size: 3905793
  relation: main_file
file_date_updated: 2020-07-14T12:47:44Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '09'
oa: 1
oa_version: Published Version
page: '4113'
pmid: 1
publication: Nature communications
publication_identifier:
  eissn:
  - 2041-1723
publication_status: published
publisher: Nature Publishing Group
quality_controlled: '1'
scopus_import: '1'
status: public
title: Biomechanical signaling within the developing zebrafish heart attunes endocardial
  growth to myocardial chamber dimensions
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 10
year: '2019'
...