[{"scopus_import":"1","corr_author":"1","publication_status":"published","article_number":"260017","oa_version":"None","abstract":[{"text":"From a simple thought to a multicellular movement","lang":"eng"}],"day":"19","publication":"Journal of Cell Science","issue":"8","external_id":{"isi":["000798123600015"],"pmid":["35438168"]},"author":[{"orcid":"0000-0002-3183-8207","full_name":"Amberg, Nicole","first_name":"Nicole","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg"},{"id":"4C9372C4-F248-11E8-B48F-1D18A9856A87","full_name":"Stouffer, Melissa A","first_name":"Melissa A","last_name":"Stouffer"},{"orcid":"0000-0001-5618-3449","full_name":"Vercellino, Irene","last_name":"Vercellino","id":"3ED6AF16-F248-11E8-B48F-1D18A9856A87","first_name":"Irene"}],"title":"Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole","department":[{"_id":"SiHi"},{"_id":"LeSa"}],"intvolume":"       135","date_updated":"2024-10-09T21:03:55Z","type":"journal_article","status":"public","publisher":"The Company of Biologists","acknowledgement":"The authors want to thank Professors Carrie Bernecky, Tom Henzinger, Martin Loose and Gaia Novarino for accepting to be interviewed, thus giving significant contribution to the discussion that lead to this article.","doi":"10.1242/jcs.260017","year":"2022","pmid":1,"_id":"12282","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","isi":1,"article_type":"letter_note","date_created":"2023-01-16T10:03:14Z","publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"quality_controlled":"1","language":[{"iso":"eng"}],"date_published":"2022-04-19T00:00:00Z","citation":{"ista":"Amberg N, Stouffer MA, Vercellino I. 2022. Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole. Journal of Cell Science. 135(8), 260017.","short":"N. Amberg, M.A. Stouffer, I. Vercellino, Journal of Cell Science 135 (2022).","apa":"Amberg, N., Stouffer, M. A., &#38; Vercellino, I. (2022). Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.260017\">https://doi.org/10.1242/jcs.260017</a>","ieee":"N. Amberg, M. A. Stouffer, and I. Vercellino, “Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole,” <i>Journal of Cell Science</i>, vol. 135, no. 8. The Company of Biologists, 2022.","mla":"Amberg, Nicole, et al. “Operation STEM Fatale – How an Equity, Diversity and Inclusion Initiative Has Brought Us to Reflect on the Current Challenges in Cell Biology and Science as a Whole.” <i>Journal of Cell Science</i>, vol. 135, no. 8, 260017, The Company of Biologists, 2022, doi:<a href=\"https://doi.org/10.1242/jcs.260017\">10.1242/jcs.260017</a>.","ama":"Amberg N, Stouffer MA, Vercellino I. Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole. <i>Journal of Cell Science</i>. 2022;135(8). doi:<a href=\"https://doi.org/10.1242/jcs.260017\">10.1242/jcs.260017</a>","chicago":"Amberg, Nicole, Melissa A Stouffer, and Irene Vercellino. “Operation STEM Fatale – How an Equity, Diversity and Inclusion Initiative Has Brought Us to Reflect on the Current Challenges in Cell Biology and Science as a Whole.” <i>Journal of Cell Science</i>. The Company of Biologists, 2022. <a href=\"https://doi.org/10.1242/jcs.260017\">https://doi.org/10.1242/jcs.260017</a>."},"month":"04","article_processing_charge":"No","volume":135},{"article_number":"259234","has_accepted_license":"1","publication_status":"published","scopus_import":"1","issue":"7","publication":"Journal of Cell Science","day":"01","abstract":[{"text":"Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone architecture remains poorly understood at nanoscales. We therefore investigated mouse hippocampal neuron growth cones using cryo-electron tomography to directly visualise their three-dimensional subcellular architecture with molecular detail. Our data showed that the hexagonal arrays of actin bundles that form filopodia penetrate and terminate deep within the growth cone interior. We directly observed the modulation of these and other growth cone actin bundles by alteration of individual F-actin helical structures. Microtubules with blunt, slightly flared or gently curved ends predominated in the growth cone, frequently contained lumenal particles and exhibited lattice defects. Investigation of the effect of absence of doublecortin, a neurodevelopmental cytoskeleton regulator, on growth cone cytoskeleton showed no major anomalies in overall growth cone organisation or in F-actin subpopulations. However, our data suggested that microtubules sustained more structural defects, highlighting the importance of microtubule integrity during growth cone migration.","lang":"eng"}],"ddc":["570"],"oa_version":"Published Version","license":"https://creativecommons.org/licenses/by/4.0/","author":[{"first_name":"Joseph","last_name":"Atherton","full_name":"Atherton, Joseph"},{"full_name":"Stouffer, Melissa A","first_name":"Melissa A","id":"4C9372C4-F248-11E8-B48F-1D18A9856A87","last_name":"Stouffer"},{"first_name":"Fiona","full_name":"Francis, Fiona","last_name":"Francis"},{"last_name":"Moores","full_name":"Moores, Carolyn A.","first_name":"Carolyn A."}],"external_id":{"isi":["000783840400010"],"pmid":["35383828"]},"file":[{"file_size":13868733,"content_type":"application/pdf","date_updated":"2023-01-30T11:41:01Z","access_level":"open_access","relation":"main_file","date_created":"2023-01-30T11:41:01Z","creator":"dernst","checksum":"4346ed32cb7c89a8ca051c7da68a9a1c","file_id":"12461","file_name":"2022_JourCellBiology_Atherton.pdf","success":1}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"department":[{"_id":"SiHi"}],"title":"Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography","doi":"10.1242/jcs.259234","acknowledgement":"J.A. was supported by a grant from the Medical Research Council (MRC), UK (MR/R000352/1) to C.A.M. Cryo-EM data were collected on equipment funded by the Wellcome Trust, UK (079605/Z/06/Z) and the Biotechnology and Biological Sciences Research Council (BBSRC) UK (BB/L014211/1). F.F.’s salary and institute were supported by Inserm (Institut National de la Santé et de la Recherche Médicale), CNRS (Centre National de la Recherche Scientifique) and Sorbonne Université. F.F.’s group was particularly supported by Agence Nationale de la\r\nRecherche (ANR-16-CE16-0011-03) and Seventh Framework Programme (EUHEALTH-\r\n2013, DESIRE, N° 60253; also funding M.S.’s salary) and the European Cooperation in Science and Technology (COST Action CA16118). Open Access funding provided by Birkbeck College: Birkbeck University of London. Deposited in PMC for immediate release.","publisher":"The Company of Biologists","status":"public","type":"journal_article","keyword":["Cell Biology"],"date_updated":"2023-08-04T10:28:34Z","intvolume":"       135","article_type":"original","isi":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"12283","pmid":1,"year":"2022","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"quality_controlled":"1","date_created":"2023-01-16T10:03:24Z","article_processing_charge":"No","volume":135,"month":"04","oa":1,"citation":{"short":"J. Atherton, M.A. Stouffer, F. Francis, C.A. Moores, Journal of Cell Science 135 (2022).","ista":"Atherton J, Stouffer MA, Francis F, Moores CA. 2022. Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. Journal of Cell Science. 135(7), 259234.","ama":"Atherton J, Stouffer MA, Francis F, Moores CA. Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. <i>Journal of Cell Science</i>. 2022;135(7). doi:<a href=\"https://doi.org/10.1242/jcs.259234\">10.1242/jcs.259234</a>","chicago":"Atherton, Joseph, Melissa A Stouffer, Fiona Francis, and Carolyn A. Moores. “Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron Tomography.” <i>Journal of Cell Science</i>. The Company of Biologists, 2022. <a href=\"https://doi.org/10.1242/jcs.259234\">https://doi.org/10.1242/jcs.259234</a>.","mla":"Atherton, Joseph, et al. “Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron Tomography.” <i>Journal of Cell Science</i>, vol. 135, no. 7, 259234, The Company of Biologists, 2022, doi:<a href=\"https://doi.org/10.1242/jcs.259234\">10.1242/jcs.259234</a>.","ieee":"J. Atherton, M. A. Stouffer, F. Francis, and C. A. Moores, “Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography,” <i>Journal of Cell Science</i>, vol. 135, no. 7. The Company of Biologists, 2022.","apa":"Atherton, J., Stouffer, M. A., Francis, F., &#38; Moores, C. A. (2022). Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.259234\">https://doi.org/10.1242/jcs.259234</a>"},"file_date_updated":"2023-01-30T11:41:01Z","date_published":"2022-04-01T00:00:00Z"},{"publication_status":"published","scopus_import":"1","article_number":"105702","has_accepted_license":"1","oa_version":"Published Version","publication":"Neurobiology of Disease","day":"15","abstract":[{"lang":"eng","text":"Human doublecortin (DCX) mutations are associated with severe brain malformations leading to aberrant neuron positioning (heterotopia), intellectual disability and epilepsy. DCX is a microtubule-associated protein which plays a key role during neurodevelopment in neuronal migration and differentiation. Dcx knockout (KO) mice show disorganized hippocampal pyramidal neurons. The CA2/CA3 pyramidal cell layer is present as two abnormal layers and disorganized CA3 KO pyramidal neurons are also more excitable than wild-type (WT) cells. To further identify abnormalities, we characterized Dcx KO hippocampal neurons at subcellular, molecular and ultrastructural levels. Severe defects were observed in mitochondria, affecting number and distribution. Also, the Golgi apparatus was visibly abnormal, increased in volume and abnormally organized. Transcriptome analyses from laser microdissected hippocampal tissue at postnatal day 60 (P60) highlighted organelle abnormalities. Ultrastructural studies of CA3 cells performed in P60 (young adult) and > 9 months (mature) tissue showed that organelle defects are persistent throughout life. Locomotor activity and fear memory of young and mature adults were also abnormal: Dcx KO mice consistently performed less well than WT littermates, with defects becoming more severe with age. Thus, we show that disruption of a neurodevelopmentally-regulated gene can lead to permanent organelle anomalies contributing to abnormal adult behavior."}],"ddc":["570"],"external_id":{"pmid":["35339680"]},"author":[{"first_name":"Melissa A","last_name":"Stouffer","id":"4C9372C4-F248-11E8-B48F-1D18A9856A87","full_name":"Stouffer, Melissa A"},{"last_name":"Khalaf-Nazzal","first_name":"R.","full_name":"Khalaf-Nazzal, R."},{"last_name":"Cifuentes-Diaz","full_name":"Cifuentes-Diaz, C.","first_name":"C."},{"last_name":"Albertini","full_name":"Albertini, G.","first_name":"G."},{"full_name":"Bandet, E.","first_name":"E.","last_name":"Bandet"},{"full_name":"Grannec, G.","first_name":"G.","last_name":"Grannec"},{"full_name":"Lavilla, V.","first_name":"V.","last_name":"Lavilla"},{"first_name":"J.-F.","full_name":"Deleuze, J.-F.","last_name":"Deleuze"},{"last_name":"Olaso","first_name":"R.","full_name":"Olaso, R."},{"full_name":"Nosten-Bertrand, M.","first_name":"M.","last_name":"Nosten-Bertrand"},{"full_name":"Francis, F.","first_name":"F.","last_name":"Francis"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","title":"Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells","file":[{"creator":"dernst","checksum":"b705d3d23d0b424ba29920be7ab64c23","file_id":"17398","access_level":"open_access","date_updated":"2024-08-06T06:54:24Z","relation":"main_file","date_created":"2024-08-06T06:54:24Z","file_name":"2022_NeurobioDisease_Stouffer.pdf","success":1,"content_type":"application/pdf","file_size":8890818}],"department":[{"_id":"SiHi"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"date_updated":"2024-08-06T06:57:39Z","publisher":"Elsevier","type":"journal_article","status":"public","intvolume":"       168","acknowledgement":"We thank Sylvie Dumont for initial aid with laser microdissection and G. Martinez-Lorenzana for experimental help with electron microscopy. We thank the animal experimentation facility and cellular and tissue imaging platforms at the Institut du Fer à Moulin, supported also by the Région Ile de France and the FRC Rotary. The Francis lab was associated with the BioPsy Labex project and the Ecole des Neurosciences de Paris Ile-de-France (ENP) network. Our salaries and lab were supported by Inserm, the Centre national de la recherche scientifique (CNRS) and Sorbonne University. The Francis group obtained the following funding contributing to this project: the European Union (EU- HEALTH-2013, DESIRE, N° 60253), the JTC 2015 Neurodevelopmental Disorders affiliated with the French Agence National de la Recherche (for \r\nNEURON8-Full- 815-006 STEM-MCD, to FF), E-Rare-3, the ERA-Net for Research on Rare Diseases affiliated with the French ANR (ERARE18-049), the European Cooperation on Science and Technology (COST Action CA16118).","doi":"10.1016/j.nbd.2022.105702","_id":"17067","pmid":1,"year":"2022","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2024-05-29T06:10:05Z","language":[{"iso":"eng"}],"quality_controlled":"1","publication_identifier":{"issn":["0969-9961"]},"file_date_updated":"2024-08-06T06:54:24Z","date_published":"2022-06-15T00:00:00Z","oa":1,"month":"06","volume":168,"article_processing_charge":"Yes","citation":{"ista":"Stouffer MA, Khalaf-Nazzal R, Cifuentes-Diaz C, Albertini G, Bandet E, Grannec G, Lavilla V, Deleuze J-F, Olaso R, Nosten-Bertrand M, Francis F. 2022. Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells. Neurobiology of Disease. 168, 105702.","short":"M.A. Stouffer, R. Khalaf-Nazzal, C. Cifuentes-Diaz, G. Albertini, E. Bandet, G. Grannec, V. Lavilla, J.-F. Deleuze, R. Olaso, M. Nosten-Bertrand, F. Francis, Neurobiology of Disease 168 (2022).","apa":"Stouffer, M. A., Khalaf-Nazzal, R., Cifuentes-Diaz, C., Albertini, G., Bandet, E., Grannec, G., … Francis, F. (2022). Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells. <i>Neurobiology of Disease</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.nbd.2022.105702\">https://doi.org/10.1016/j.nbd.2022.105702</a>","ieee":"M. A. Stouffer <i>et al.</i>, “Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells,” <i>Neurobiology of Disease</i>, vol. 168. Elsevier, 2022.","mla":"Stouffer, Melissa A., et al. “Doublecortin Mutation Leads to Persistent Defects in the Golgi Apparatus and Mitochondria in Adult Hippocampal Pyramidal Cells.” <i>Neurobiology of Disease</i>, vol. 168, 105702, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.nbd.2022.105702\">10.1016/j.nbd.2022.105702</a>.","chicago":"Stouffer, Melissa A, R. Khalaf-Nazzal, C. Cifuentes-Diaz, G. Albertini, E. Bandet, G. Grannec, V. Lavilla, et al. “Doublecortin Mutation Leads to Persistent Defects in the Golgi Apparatus and Mitochondria in Adult Hippocampal Pyramidal Cells.” <i>Neurobiology of Disease</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.nbd.2022.105702\">https://doi.org/10.1016/j.nbd.2022.105702</a>.","ama":"Stouffer MA, Khalaf-Nazzal R, Cifuentes-Diaz C, et al. Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells. <i>Neurobiology of Disease</i>. 2022;168. doi:<a href=\"https://doi.org/10.1016/j.nbd.2022.105702\">10.1016/j.nbd.2022.105702</a>"}}]