---
_id: '12282'
abstract:
- lang: eng
text: From a simple thought to a multicellular movement
acknowledgement: The authors want to thank Professors Carrie Bernecky, Tom Henzinger,
Martin Loose and Gaia Novarino for accepting to be interviewed, thus giving significant
contribution to the discussion that lead to this article.
article_number: '260017'
article_processing_charge: No
article_type: letter_note
author:
- first_name: Nicole
full_name: Amberg, Nicole
id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
last_name: Amberg
orcid: 0000-0002-3183-8207
- first_name: Melissa A
full_name: Stouffer, Melissa A
id: 4C9372C4-F248-11E8-B48F-1D18A9856A87
last_name: Stouffer
- first_name: Irene
full_name: Vercellino, Irene
id: 3ED6AF16-F248-11E8-B48F-1D18A9856A87
last_name: Vercellino
orcid: 0000-0001-5618-3449
citation:
ama: Amberg N, Stouffer MA, Vercellino I. Operation STEM fatale – how an equity,
diversity and inclusion initiative has brought us to reflect on the current challenges
in cell biology and science as a whole. Journal of Cell Science. 2022;135(8).
doi:10.1242/jcs.260017
apa: Amberg, N., Stouffer, M. A., & Vercellino, I. (2022). Operation STEM fatale
– how an equity, diversity and inclusion initiative has brought us to reflect
on the current challenges in cell biology and science as a whole. Journal of
Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.260017
chicago: Amberg, Nicole, Melissa A Stouffer, and Irene Vercellino. “Operation STEM
Fatale – How an Equity, Diversity and Inclusion Initiative Has Brought Us to Reflect
on the Current Challenges in Cell Biology and Science as a Whole.” Journal
of Cell Science. The Company of Biologists, 2022. https://doi.org/10.1242/jcs.260017.
ieee: N. Amberg, M. A. Stouffer, and I. Vercellino, “Operation STEM fatale – how
an equity, diversity and inclusion initiative has brought us to reflect on the
current challenges in cell biology and science as a whole,” Journal of Cell
Science, vol. 135, no. 8. The Company of Biologists, 2022.
ista: Amberg N, Stouffer MA, Vercellino I. 2022. Operation STEM fatale – how an
equity, diversity and inclusion initiative has brought us to reflect on the current
challenges in cell biology and science as a whole. Journal of Cell Science. 135(8),
260017.
mla: Amberg, Nicole, et al. “Operation STEM Fatale – How an Equity, Diversity and
Inclusion Initiative Has Brought Us to Reflect on the Current Challenges in Cell
Biology and Science as a Whole.” Journal of Cell Science, vol. 135, no.
8, 260017, The Company of Biologists, 2022, doi:10.1242/jcs.260017.
short: N. Amberg, M.A. Stouffer, I. Vercellino, Journal of Cell Science 135 (2022).
corr_author: '1'
date_created: 2023-01-16T10:03:14Z
date_published: 2022-04-19T00:00:00Z
date_updated: 2024-10-09T21:03:55Z
day: '19'
department:
- _id: SiHi
- _id: LeSa
doi: 10.1242/jcs.260017
external_id:
isi:
- '000798123600015'
pmid:
- '35438168'
intvolume: ' 135'
isi: 1
issue: '8'
language:
- iso: eng
month: '04'
oa_version: None
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Operation STEM fatale – how an equity, diversity and inclusion initiative has
brought us to reflect on the current challenges in cell biology and science as a
whole
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 135
year: '2022'
...
---
_id: '12283'
abstract:
- lang: eng
text: Neurons extend axons to form the complex circuitry of the mature brain. This
depends on the coordinated response and continuous remodelling of the microtubule
and F-actin networks in the axonal growth cone. Growth cone architecture remains
poorly understood at nanoscales. We therefore investigated mouse hippocampal neuron
growth cones using cryo-electron tomography to directly visualise their three-dimensional
subcellular architecture with molecular detail. Our data showed that the hexagonal
arrays of actin bundles that form filopodia penetrate and terminate deep within
the growth cone interior. We directly observed the modulation of these and other
growth cone actin bundles by alteration of individual F-actin helical structures.
Microtubules with blunt, slightly flared or gently curved ends predominated in
the growth cone, frequently contained lumenal particles and exhibited lattice
defects. Investigation of the effect of absence of doublecortin, a neurodevelopmental
cytoskeleton regulator, on growth cone cytoskeleton showed no major anomalies
in overall growth cone organisation or in F-actin subpopulations. However, our
data suggested that microtubules sustained more structural defects, highlighting
the importance of microtubule integrity during growth cone migration.
acknowledgement: "J.A. was supported by a grant from the Medical Research Council
(MRC), UK (MR/R000352/1) to C.A.M. Cryo-EM data were collected on equipment funded
by the Wellcome Trust, UK (079605/Z/06/Z) and the Biotechnology and Biological Sciences
Research Council (BBSRC) UK (BB/L014211/1). F.F.’s salary and institute were supported
by Inserm (Institut National de la Santé et de la Recherche Médicale), CNRS (Centre
National de la Recherche Scientifique) and Sorbonne Université. F.F.’s group was
particularly supported by Agence Nationale de la\r\nRecherche (ANR-16-CE16-0011-03)
and Seventh Framework Programme (EUHEALTH-\r\n2013, DESIRE, N° 60253; also funding
M.S.’s salary) and the European Cooperation in Science and Technology (COST Action
CA16118). Open Access funding provided by Birkbeck College: Birkbeck University
of London. Deposited in PMC for immediate release."
article_number: '259234'
article_processing_charge: No
article_type: original
author:
- first_name: Joseph
full_name: Atherton, Joseph
last_name: Atherton
- first_name: Melissa A
full_name: Stouffer, Melissa A
id: 4C9372C4-F248-11E8-B48F-1D18A9856A87
last_name: Stouffer
- first_name: Fiona
full_name: Francis, Fiona
last_name: Francis
- first_name: Carolyn A.
full_name: Moores, Carolyn A.
last_name: Moores
citation:
ama: Atherton J, Stouffer MA, Francis F, Moores CA. Visualising the cytoskeletal
machinery in neuronal growth cones using cryo-electron tomography. Journal
of Cell Science. 2022;135(7). doi:10.1242/jcs.259234
apa: Atherton, J., Stouffer, M. A., Francis, F., & Moores, C. A. (2022). Visualising
the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography.
Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.259234
chicago: Atherton, Joseph, Melissa A Stouffer, Fiona Francis, and Carolyn A. Moores.
“Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron
Tomography.” Journal of Cell Science. The Company of Biologists, 2022.
https://doi.org/10.1242/jcs.259234.
ieee: J. Atherton, M. A. Stouffer, F. Francis, and C. A. Moores, “Visualising the
cytoskeletal machinery in neuronal growth cones using cryo-electron tomography,”
Journal of Cell Science, vol. 135, no. 7. The Company of Biologists, 2022.
ista: Atherton J, Stouffer MA, Francis F, Moores CA. 2022. Visualising the cytoskeletal
machinery in neuronal growth cones using cryo-electron tomography. Journal of
Cell Science. 135(7), 259234.
mla: Atherton, Joseph, et al. “Visualising the Cytoskeletal Machinery in Neuronal
Growth Cones Using Cryo-Electron Tomography.” Journal of Cell Science,
vol. 135, no. 7, 259234, The Company of Biologists, 2022, doi:10.1242/jcs.259234.
short: J. Atherton, M.A. Stouffer, F. Francis, C.A. Moores, Journal of Cell Science
135 (2022).
date_created: 2023-01-16T10:03:24Z
date_published: 2022-04-01T00:00:00Z
date_updated: 2023-08-04T10:28:34Z
day: '01'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1242/jcs.259234
external_id:
isi:
- '000783840400010'
pmid:
- '35383828'
file:
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checksum: 4346ed32cb7c89a8ca051c7da68a9a1c
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T11:41:01Z
date_updated: 2023-01-30T11:41:01Z
file_id: '12461'
file_name: 2022_JourCellBiology_Atherton.pdf
file_size: 13868733
relation: main_file
success: 1
file_date_updated: 2023-01-30T11:41:01Z
has_accepted_license: '1'
intvolume: ' 135'
isi: 1
issue: '7'
keyword:
- Cell Biology
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron
tomography
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 135
year: '2022'
...
---
_id: '17067'
abstract:
- lang: eng
text: 'Human doublecortin (DCX) mutations are associated with severe brain malformations
leading to aberrant neuron positioning (heterotopia), intellectual disability
and epilepsy. DCX is a microtubule-associated protein which plays a key role during
neurodevelopment in neuronal migration and differentiation. Dcx knockout (KO)
mice show disorganized hippocampal pyramidal neurons. The CA2/CA3 pyramidal cell
layer is present as two abnormal layers and disorganized CA3 KO pyramidal neurons
are also more excitable than wild-type (WT) cells. To further identify abnormalities,
we characterized Dcx KO hippocampal neurons at subcellular, molecular and ultrastructural
levels. Severe defects were observed in mitochondria, affecting number and distribution.
Also, the Golgi apparatus was visibly abnormal, increased in volume and abnormally
organized. Transcriptome analyses from laser microdissected hippocampal tissue
at postnatal day 60 (P60) highlighted organelle abnormalities. Ultrastructural
studies of CA3 cells performed in P60 (young adult) and > 9 months (mature) tissue
showed that organelle defects are persistent throughout life. Locomotor activity
and fear memory of young and mature adults were also abnormal: Dcx KO mice consistently
performed less well than WT littermates, with defects becoming more severe with
age. Thus, we show that disruption of a neurodevelopmentally-regulated gene can
lead to permanent organelle anomalies contributing to abnormal adult behavior.'
acknowledgement: "We thank Sylvie Dumont for initial aid with laser microdissection
and G. Martinez-Lorenzana for experimental help with electron microscopy. We thank
the animal experimentation facility and cellular and tissue imaging platforms at
the Institut du Fer à Moulin, supported also by the Région Ile de France and the
FRC Rotary. The Francis lab was associated with the BioPsy Labex project and the
Ecole des Neurosciences de Paris Ile-de-France (ENP) network. Our salaries and lab
were supported by Inserm, the Centre national de la recherche scientifique (CNRS)
and Sorbonne University. The Francis group obtained the following funding contributing
to this project: the European Union (EU- HEALTH-2013, DESIRE, N° 60253), the JTC
2015 Neurodevelopmental Disorders affiliated with the French Agence National de
la Recherche (for \r\nNEURON8-Full- 815-006 STEM-MCD, to FF), E-Rare-3, the ERA-Net
for Research on Rare Diseases affiliated with the French ANR (ERARE18-049), the
European Cooperation on Science and Technology (COST Action CA16118)."
article_number: '105702'
article_processing_charge: Yes
article_type: original
author:
- first_name: Melissa A
full_name: Stouffer, Melissa A
id: 4C9372C4-F248-11E8-B48F-1D18A9856A87
last_name: Stouffer
- first_name: R.
full_name: Khalaf-Nazzal, R.
last_name: Khalaf-Nazzal
- first_name: C.
full_name: Cifuentes-Diaz, C.
last_name: Cifuentes-Diaz
- first_name: G.
full_name: Albertini, G.
last_name: Albertini
- first_name: E.
full_name: Bandet, E.
last_name: Bandet
- first_name: G.
full_name: Grannec, G.
last_name: Grannec
- first_name: V.
full_name: Lavilla, V.
last_name: Lavilla
- first_name: J.-F.
full_name: Deleuze, J.-F.
last_name: Deleuze
- first_name: R.
full_name: Olaso, R.
last_name: Olaso
- first_name: M.
full_name: Nosten-Bertrand, M.
last_name: Nosten-Bertrand
- first_name: F.
full_name: Francis, F.
last_name: Francis
citation:
ama: Stouffer MA, Khalaf-Nazzal R, Cifuentes-Diaz C, et al. Doublecortin mutation
leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal
pyramidal cells. Neurobiology of Disease. 2022;168. doi:10.1016/j.nbd.2022.105702
apa: Stouffer, M. A., Khalaf-Nazzal, R., Cifuentes-Diaz, C., Albertini, G., Bandet,
E., Grannec, G., … Francis, F. (2022). Doublecortin mutation leads to persistent
defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal
cells. Neurobiology of Disease. Elsevier. https://doi.org/10.1016/j.nbd.2022.105702
chicago: Stouffer, Melissa A, R. Khalaf-Nazzal, C. Cifuentes-Diaz, G. Albertini,
E. Bandet, G. Grannec, V. Lavilla, et al. “Doublecortin Mutation Leads to Persistent
Defects in the Golgi Apparatus and Mitochondria in Adult Hippocampal Pyramidal
Cells.” Neurobiology of Disease. Elsevier, 2022. https://doi.org/10.1016/j.nbd.2022.105702.
ieee: M. A. Stouffer et al., “Doublecortin mutation leads to persistent defects
in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells,”
Neurobiology of Disease, vol. 168. Elsevier, 2022.
ista: Stouffer MA, Khalaf-Nazzal R, Cifuentes-Diaz C, Albertini G, Bandet E, Grannec
G, Lavilla V, Deleuze J-F, Olaso R, Nosten-Bertrand M, Francis F. 2022. Doublecortin
mutation leads to persistent defects in the Golgi apparatus and mitochondria in
adult hippocampal pyramidal cells. Neurobiology of Disease. 168, 105702.
mla: Stouffer, Melissa A., et al. “Doublecortin Mutation Leads to Persistent Defects
in the Golgi Apparatus and Mitochondria in Adult Hippocampal Pyramidal Cells.”
Neurobiology of Disease, vol. 168, 105702, Elsevier, 2022, doi:10.1016/j.nbd.2022.105702.
short: M.A. Stouffer, R. Khalaf-Nazzal, C. Cifuentes-Diaz, G. Albertini, E. Bandet,
G. Grannec, V. Lavilla, J.-F. Deleuze, R. Olaso, M. Nosten-Bertrand, F. Francis,
Neurobiology of Disease 168 (2022).
date_created: 2024-05-29T06:10:05Z
date_published: 2022-06-15T00:00:00Z
date_updated: 2024-08-06T06:57:39Z
day: '15'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.nbd.2022.105702
external_id:
pmid:
- '35339680'
file:
- access_level: open_access
checksum: b705d3d23d0b424ba29920be7ab64c23
content_type: application/pdf
creator: dernst
date_created: 2024-08-06T06:54:24Z
date_updated: 2024-08-06T06:54:24Z
file_id: '17398'
file_name: 2022_NeurobioDisease_Stouffer.pdf
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success: 1
file_date_updated: 2024-08-06T06:54:24Z
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intvolume: ' 168'
language:
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license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Neurobiology of Disease
publication_identifier:
issn:
- 0969-9961
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Doublecortin mutation leads to persistent defects in the Golgi apparatus and
mitochondria in adult hippocampal pyramidal cells
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
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year: '2022'
...