[{"type":"journal_article","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"date_updated":"2026-04-28T13:15:42Z","day":"20","date_created":"2026-04-26T22:01:46Z","department":[{"_id":"AnSa"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","publication_status":"published","issue":"8","volume":36,"OA_place":"publisher","oa":1,"page":"1903-1917.e5","external_id":{"pmid":["41881011"]},"acknowledgement":"S.B. acknowledges support from the National Institutes of Health (NIH R35 GM143042) and the National Science Foundation (NSF MCB-2203601). G.L.G. acknowledges support from the Wellcome Trust (211112/Z/18/Z), the Royal Society (RG\\R2\\232082), and the Leverhulme Trust (RPG-2024-147). E.M. acknowledges support from European Union’s Horizon 2021 Marie Sklodowska-Curie grant agreement no. 101067028. F.P.-V. acknowledges support from the NOMIS foundation. The surface subtraction macro is courtesy of Dr. Dale Moulding and available on GitHub (https://github.com/DaleMoulding/Fiji-Macros).","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"quality_controlled":"1","scopus_import":"1","month":"04","title":"Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis","publication":"Current Biology","file_date_updated":"2026-04-28T13:13:40Z","_id":"21761","language":[{"iso":"eng"}],"status":"public","has_accepted_license":"1","author":[{"first_name":"Fernanda L","full_name":"Perez Verdugo, Fernanda L","last_name":"Perez Verdugo","id":"4ecec223-9070-11ef-a0a9-bc76077bea8d"},{"last_name":"Maniou","full_name":"Maniou, Eirini","first_name":"Eirini"},{"first_name":"Gabriel L.","full_name":"Galea, Gabriel L.","last_name":"Galea"},{"first_name":"Shiladitya","last_name":"Banerjee","full_name":"Banerjee, Shiladitya"}],"citation":{"ista":"Perez Verdugo FL, Maniou E, Galea GL, Banerjee S. 2026. Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis. Current Biology. 36(8), 1903–1917.e5.","short":"F.L. Perez Verdugo, E. Maniou, G.L. Galea, S. Banerjee, Current Biology 36 (2026) 1903–1917.e5.","chicago":"Perez Verdugo, Fernanda L, Eirini Maniou, Gabriel L. Galea, and Shiladitya Banerjee. “Mechanosensitive Feedback Organizes Cell Shape and Motion during Hindbrain Neuropore Morphogenesis.” <i>Current Biology</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">https://doi.org/10.1016/j.cub.2026.02.068</a>.","ama":"Perez Verdugo FL, Maniou E, Galea GL, Banerjee S. Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis. <i>Current Biology</i>. 2026;36(8):1903-1917.e5. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">10.1016/j.cub.2026.02.068</a>","mla":"Perez Verdugo, Fernanda L., et al. “Mechanosensitive Feedback Organizes Cell Shape and Motion during Hindbrain Neuropore Morphogenesis.” <i>Current Biology</i>, vol. 36, no. 8, Elsevier, 2026, p. 1903–1917.e5, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">10.1016/j.cub.2026.02.068</a>.","apa":"Perez Verdugo, F. L., Maniou, E., Galea, G. L., &#38; Banerjee, S. (2026). Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">https://doi.org/10.1016/j.cub.2026.02.068</a>","ieee":"F. L. Perez Verdugo, E. Maniou, G. L. Galea, and S. Banerjee, “Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis,” <i>Current Biology</i>, vol. 36, no. 8. Elsevier, p. 1903–1917.e5, 2026."},"intvolume":"        36","OA_type":"hybrid","year":"2026","date_published":"2026-04-20T00:00:00Z","pmid":1,"file":[{"checksum":"80ae45457b4682c50c84f54de15aa9a8","content_type":"application/pdf","success":1,"access_level":"open_access","relation":"main_file","date_created":"2026-04-28T13:13:40Z","file_name":"2026_CurrentBiology_PerezVerdugo.pdf","file_size":13402043,"date_updated":"2026-04-28T13:13:40Z","creator":"dernst","file_id":"21774"}],"abstract":[{"text":"Neural tube closure is a critical morphogenetic process in vertebrate development, and failure to close cranial regions such as the hindbrain neuropore (HNP) leads to severe congenital malformations. While mechanical forces such as actomyosin purse-string contraction and directional cell crawling have been implicated in driving HNP closure, how these forces organize local cell shape and motion to produce large-scale tissue remodeling remains poorly understood. Using live and fixed imaging of mouse embryos combined with cell-based biophysical modeling, we show that these force-generating mechanisms are insufficient to explain the reproducible patterns of cell elongation and nematic alignment observed at the HNP border. Instead, we show that local anisotropic stress and cytoskeletal organization are required to generate these patterns and promote midline cell motion. Our model captures key features of cell shape dynamics and emergent nematic order, which we confirm experimentally, including the alignment of actin fibers with cell shape and enhanced midline cell speed. Comparative analysis with chick embryos, which lack supracellular purse strings, supports a conserved link between tension generation and cellular patterning. These findings establish a physical framework connecting force generation, cell shape anisotropy, and tissue morphodynamics during epithelial gap closure.","lang":"eng"}],"oa_version":"Published Version","ddc":["570"],"doi":"10.1016/j.cub.2026.02.068","publisher":"Elsevier","article_processing_charge":"Yes (in subscription journal)"},{"oa_version":"Published Version","corr_author":"1","doi":"10.1103/gdd5-rnh7","ddc":["570"],"abstract":[{"text":"Collagen IV is one of the main components of the basement membrane, a layer of material that lines the majority of tissues in multicellular organisms. Collagen IV molecules assemble into networks, providing stiffness and elasticity to tissues and informing cell and organ shape, especially during development. In this work, we develop two coarse-grained models for collagen IV molecules that retain biochemical bond specificity and coarse grain at different length scales. Through molecular-dynamics simulations, we test the assembly and mechanics of the resulting networks and measure their response to strain in terms of stress, microscopic alignment, and bond dynamics. Within the basement membrane, collagen IV networks rearrange by molecule turnover, which affects tissue organization and can be linked with enzyme activity. Here we explore network rearrangements via bond remodeling, the process of breaking and remaking of bonds between network molecules. We then investigate the effects of active (enzymatic) bond remodeling. We find that this nonequilibrium remodeling allows a network to keep its integrity under strain, while relaxing fully over a variety of timescales, a dynamic response that is unavailable to networks undergoing equilibrium remodeling.","lang":"eng"}],"file":[{"creator":"dernst","file_id":"21308","file_size":2277704,"date_updated":"2026-02-17T13:36:01Z","access_level":"open_access","relation":"main_file","date_created":"2026-02-17T13:36:01Z","file_name":"2025_PRXLife_Meadowcroft.pdf","checksum":"04cae5231d97e533145c493880fadbd9","content_type":"application/pdf","success":1}],"publisher":"American Physical Society","article_processing_charge":"Yes","citation":{"apa":"Meadowcroft, B., Sorichetti, V., Ratajczyk, E., Perez Verdugo, F. L., Khalilgharibi, N., Mao, Y., … Šarić, A. (2025). Nonequilibrium remodeling of collagen IV networks in Silico. <i>PRX Life</i>. American Physical Society. <a href=\"https://doi.org/10.1103/gdd5-rnh7\">https://doi.org/10.1103/gdd5-rnh7</a>","ieee":"B. Meadowcroft <i>et al.</i>, “Nonequilibrium remodeling of collagen IV networks in Silico,” <i>PRX Life</i>, vol. 3. American Physical Society, 2025.","mla":"Meadowcroft, Billie, et al. “Nonequilibrium Remodeling of Collagen IV Networks in Silico.” <i>PRX Life</i>, vol. 3, 033019, American Physical Society, 2025, doi:<a href=\"https://doi.org/10.1103/gdd5-rnh7\">10.1103/gdd5-rnh7</a>.","ama":"Meadowcroft B, Sorichetti V, Ratajczyk E, et al. Nonequilibrium remodeling of collagen IV networks in Silico. <i>PRX Life</i>. 2025;3. doi:<a href=\"https://doi.org/10.1103/gdd5-rnh7\">10.1103/gdd5-rnh7</a>","chicago":"Meadowcroft, Billie, Valerio Sorichetti, Eryk Ratajczyk, Fernanda L Perez Verdugo, Nargess Khalilgharibi, Yanlan Mao, Ivan Palaia, and Anđela Šarić. “Nonequilibrium Remodeling of Collagen IV Networks in Silico.” <i>PRX Life</i>. American Physical Society, 2025. <a href=\"https://doi.org/10.1103/gdd5-rnh7\">https://doi.org/10.1103/gdd5-rnh7</a>.","short":"B. Meadowcroft, V. Sorichetti, E. Ratajczyk, F.L. Perez Verdugo, N. Khalilgharibi, Y. Mao, I. Palaia, A. Šarić, PRX Life 3 (2025).","ista":"Meadowcroft B, Sorichetti V, Ratajczyk E, Perez Verdugo FL, Khalilgharibi N, Mao Y, Palaia I, Šarić A. 2025. Nonequilibrium remodeling of collagen IV networks in Silico. PRX Life. 3, 033019."},"author":[{"orcid":"0000-0003-3441-1337","first_name":"Billie","full_name":"Meadowcroft, Billie","last_name":"Meadowcroft","id":"a4725fd6-932b-11ed-81e2-c098c7f37ae1"},{"orcid":"0000-0002-9645-6576","first_name":"Valerio","last_name":"Sorichetti","full_name":"Sorichetti, Valerio","id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b"},{"last_name":"Ratajczyk","full_name":"Ratajczyk, Eryk","first_name":"Eryk"},{"full_name":"Perez Verdugo, Fernanda L","last_name":"Perez Verdugo","id":"4ecec223-9070-11ef-a0a9-bc76077bea8d","first_name":"Fernanda L"},{"first_name":"Nargess","full_name":"Khalilgharibi, Nargess","last_name":"Khalilgharibi"},{"last_name":"Mao","full_name":"Mao, Yanlan","first_name":"Yanlan"},{"orcid":" 0000-0002-8843-9485 ","first_name":"Ivan","full_name":"Palaia, Ivan","last_name":"Palaia","id":"9c805cd2-4b75-11ec-a374-db6dd0ed57fa"},{"first_name":"Anđela","orcid":"0000-0002-7854-2139","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","full_name":"Šarić, Anđela","last_name":"Šarić"}],"intvolume":"         3","status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"ec_funded":1,"date_published":"2025-09-05T00:00:00Z","year":"2025","OA_type":"gold","PlanS_conform":"1","publication_identifier":{"eissn":["2835-8279"]},"acknowledgement":"This work received funding from the European Research Council under the European Union's Horizon 2020 research and innovation program through Grant Agreement No. 802960 (B.M., V.S., I.P., and A.Š.), the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 101034413 (I.P.), the NOMIS Foundation (F.P.-V.), the National Centre for the Replacement, Refinement and Reduction of Animals in Research Grant No. NC/T002425/1 (N.K.), Leverhulme Trust project Grant No. RPG-2020-068 (N.K.), MRC Fellowship No. MR/W027437/1 (Y.M.), a Lister Institute Research Prize (Y.M.) and EMBO Young Investigator Programme (Y.M. and A.Š.).","quality_controlled":"1","article_number":"033019","volume":3,"oa":1,"OA_place":"publisher","_id":"21256","publication":"PRX Life","file_date_updated":"2026-02-17T13:36:01Z","title":"Nonequilibrium remodeling of collagen IV networks in Silico","month":"09","date_updated":"2026-02-17T13:37:38Z","project":[{"call_identifier":"H2020","grant_number":"802960","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e"},{"call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","name":"IST-BRIDGE: International postdoctoral program","grant_number":"101034413"},{"_id":"349b6ff1-11ca-11ed-8bc3-f006047c2eeb","name":"EMBO Young Investigator Program - Andela Saric"}],"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"AnSa"}],"publication_status":"published","date_created":"2026-02-16T15:55:03Z","day":"05","DOAJ_listed":"1"}]