---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '22297'
abstract:
- lang: eng
  text: 'Determination of the rheological properties of cells is known to require
    active measurements, which largely depend on the internalization of mechanical
    probes. Here, we circumvent this problem via the introduction of Rheological focused
    light-induced cytoplasmic streaming (Rheo-FLUCS): an active, yet probe-free approach
    that leverages light-induced flows to access mechanical changes in complex systems.
    While Rheo-FLUCS is facilitated by thermoviscous expansion phenomena rather than
    external forces, here we show equivalence in its ability to measure relative viscoelastic
    properties. Specifically, we demonstrate a phase-lag equivalence with probe-dependent
    active microrheology in a wide range of physically different, yet chemically identical
    materials. We exemplify the utility of Rheo-FLUCS in three distinctly different
    biological systems: compound-treated mouse fibroblasts (NIH-3T3), genetically
    modified human osteoblasts (U2OS) to elucidate the role of myosins in cytoplasmic
    mechanics, and early ascidian oocytes of Phallusia mammillata at fertilization
    stage. Our biological use-cases exemplify the application versatility of Rheo-FLUCS,
    which in the future may use phase information as a marker for developmental success.'
acknowledgement: The authors gratefully acknowledge help from Dr. Benjamin Seelbinder,
  Mr. Claudius George, and Mr. Falk Elsner in designing and constructing the magnetic
  needle for force-driven rheology experiments. We thank Dr. Iain Patten for valuable
  discussions on the structure of the manuscript and Mr. Ivan Saraev for graphics
  support. I.D.S. and M.K. kindly acknowledge funding from the Life grant by Volkswagen
  Foundation (Life! grant no. 92772), Deutsche Forschungsgemeinschaft (DFG, German
  Research Foundation, Germany's Excellence Strategy, 2082/1 and grant no. 515462906),
  and the Hector Foundation. I.D.S. was additionally funded by the Karlsruhe Institute
  of Technology (Excellence Strategy via the Young Investigator Group Preparation
  Program). V.R.K., S.W., and M.K. thank the ERC Starting Grant GHOSTs (grant no.
  853619).
article_number: pgag190
article_processing_charge: Yes
article_type: original
author:
- first_name: Iliya D
  full_name: Stoev, Iliya D
  last_name: Stoev
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Antonio
  full_name: Minopoli, Antonio
  last_name: Minopoli
- first_name: Susan
  full_name: Wagner, Susan
  last_name: Wagner
- first_name: Venkat Raghavan
  full_name: Krishnaswamy, Venkat Raghavan
  last_name: Krishnaswamy
- first_name: Elena
  full_name: Erben, Elena
  last_name: Erben
- first_name: Kai
  full_name: Weißenbruch, Kai
  last_name: Weißenbruch
- first_name: Nicola
  full_name: Maghelli, Nicola
  last_name: Maghelli
- first_name: Martin
  full_name: Bastmeyer, Martin
  last_name: Bastmeyer
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Moritz
  full_name: Kreysing, Moritz
  last_name: Kreysing
biorxivid: 1
citation:
  ama: Stoev ID, Bolger-Munro M, Minopoli A, et al. Active and probe-free intracellular
    rheology via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. 2026;5(6).
    doi:<a href="https://doi.org/10.1093/pnasnexus/pgag190">10.1093/pnasnexus/pgag190</a>
  apa: Stoev, I. D., Bolger-Munro, M., Minopoli, A., Wagner, S., Krishnaswamy, V.
    R., Erben, E., … Kreysing, M. (2026). Active and probe-free intracellular rheology
    via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. Oxford University
    Press. <a href="https://doi.org/10.1093/pnasnexus/pgag190">https://doi.org/10.1093/pnasnexus/pgag190</a>
  chicago: Stoev, Iliya D, Madison Bolger-Munro, Antonio Minopoli, Susan Wagner, Venkat
    Raghavan Krishnaswamy, Elena Erben, Kai Weißenbruch, et al. “Active and Probe-Free
    Intracellular Rheology via Phase-Sensitive Thermoviscous Flows.” <i>PNAS Nexus</i>.
    Oxford University Press, 2026. <a href="https://doi.org/10.1093/pnasnexus/pgag190">https://doi.org/10.1093/pnasnexus/pgag190</a>.
  ieee: I. D. Stoev <i>et al.</i>, “Active and probe-free intracellular rheology via
    phase-sensitive thermoviscous flows,” <i>PNAS Nexus</i>, vol. 5, no. 6. Oxford
    University Press, 2026.
  ista: Stoev ID, Bolger-Munro M, Minopoli A, Wagner S, Krishnaswamy VR, Erben E,
    Weißenbruch K, Maghelli N, Bastmeyer M, Heisenberg C-PJ, Kreysing M. 2026. Active
    and probe-free intracellular rheology via phase-sensitive thermoviscous flows.
    PNAS Nexus. 5(6), pgag190.
  mla: Stoev, Iliya D., et al. “Active and Probe-Free Intracellular Rheology via Phase-Sensitive
    Thermoviscous Flows.” <i>PNAS Nexus</i>, vol. 5, no. 6, pgag190, Oxford University
    Press, 2026, doi:<a href="https://doi.org/10.1093/pnasnexus/pgag190">10.1093/pnasnexus/pgag190</a>.
  short: I.D. Stoev, M. Bolger-Munro, A. Minopoli, S. Wagner, V.R. Krishnaswamy, E.
    Erben, K. Weißenbruch, N. Maghelli, M. Bastmeyer, C.-P.J. Heisenberg, M. Kreysing,
    PNAS Nexus 5 (2026).
das_tickbox: '1'
dataavailabilitystatement: 'All data and materials are made available on Zenodo public
  repository under Creative Commons Attribution 4.0 International License: https://doi.org/10.5281/zenodo.20322020.'
date_created: 2026-07-13T09:50:12Z
date_published: 2026-06-01T00:00:00Z
date_updated: 2026-07-13T13:14:43Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1093/pnasnexus/pgag190
external_id:
  biorxivid:
  - 10.1101/2025.04.07.647540
file:
- access_level: open_access
  checksum: bb2c89ea73da762ad6423ac299ac3533
  content_type: application/pdf
  creator: dernst
  date_created: 2026-07-13T13:13:56Z
  date_updated: 2026-07-13T13:13:56Z
  file_id: '22312'
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  file_size: 3073062
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  success: 1
file_date_updated: 2026-07-13T13:13:56Z
fulldoi: https://doi.org/10.1093/pnasnexus/pgag190
has_accepted_license: '1'
intvolume: '         5'
issue: '6'
keyword:
- cell mechanics
- active microrheology
- noninvasiveness
- thermoviscous flows
- FLUCS
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '06'
oa: 1
oa_version: Published Version
publication: PNAS Nexus
publication_identifier:
  eissn:
  - 2752-6542
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Active and probe-free intracellular rheology via phase-sensitive thermoviscous
  flows
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2026'
...
---
_id: '14846'
abstract:
- lang: eng
  text: Contraction and flow of the actin cell cortex have emerged as a common principle
    by which cells reorganize their cytoplasm and take shape. However, how these cortical
    flows interact with adjacent cytoplasmic components, changing their form and localization,
    and how this affects cytoplasmic organization and cell shape remains unclear.
    Here we show that in ascidian oocytes, the cooperative activities of cortical
    actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive
    oocyte cytoplasmic reorganization and shape changes following fertilization. We
    show that vegetal-directed cortical actomyosin flows, established upon oocyte
    fertilization, lead to both the accumulation of cortical actin at the vegetal
    pole of the zygote and compression and local buckling of the adjacent elastic
    solid-like myoplasm layer due to friction forces generated at their interface.
    Once cortical flows have ceased, the multiple myoplasm buckles resolve into one
    larger buckle, which again drives the formation of the contraction pole—a protuberance
    of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings
    reveal a mechanism where cortical actomyosin network flows determine cytoplasmic
    reorganization and cell shape by deforming adjacent cytoplasmic components through
    friction forces.
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: NanoFab
acknowledgement: We would like to thank A. McDougall, E. Hannezo and the Heisenberg
  lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP
  and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific
  Service Units of the Institute of Science and Technology Austria through resources
  provided by the Electron Microscopy Facility, Imaging and Optics Facility and the
  Nanofabrication Facility. This work was supported by a Joint Project Grant from
  the FWF (I 3601-B27).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Silvia
  full_name: Caballero Mancebo, Silvia
  id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
  last_name: Caballero Mancebo
  orcid: 0000-0002-5223-3346
- first_name: Rushikesh
  full_name: Shinde, Rushikesh
  last_name: Shinde
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Matilda
  full_name: Peruzzo, Matilda
  id: 3F920B30-F248-11E8-B48F-1D18A9856A87
  last_name: Peruzzo
  orcid: 0000-0002-3415-4628
- first_name: Gregory
  full_name: Szep, Gregory
  id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
  last_name: Szep
- first_name: Irene
  full_name: Steccari, Irene
  id: 2705C766-9FE2-11EA-B224-C6773DDC885E
  last_name: Steccari
- first_name: David
  full_name: Labrousse Arias, David
  id: CD573DF4-9ED3-11E9-9D77-3223E6697425
  last_name: Labrousse Arias
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Andrew
  full_name: Callan-Jones, Andrew
  last_name: Callan-Jones
- first_name: Raphaël
  full_name: Voituriez, Raphaël
  last_name: Voituriez
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine
    cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization.
    <i>Nature Physics</i>. 2024;20:310-321. doi:<a href="https://doi.org/10.1038/s41567-023-02302-1">10.1038/s41567-023-02302-1</a>
  apa: Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G.,
    Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic
    reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature
    Physics</i>. Springer Nature. <a href="https://doi.org/10.1038/s41567-023-02302-1">https://doi.org/10.1038/s41567-023-02302-1</a>
  chicago: Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda
    Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction
    Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes
    upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href="https://doi.org/10.1038/s41567-023-02302-1">https://doi.org/10.1038/s41567-023-02302-1</a>.
  ieee: S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic
    reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature
    Physics</i>, vol. 20. Springer Nature, pp. 310–321, 2024.
  ista: Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari
    I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg
    C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes
    of ascidian oocytes upon fertilization. Nature Physics. 20, 310–321.
  mla: Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization
    and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>,
    vol. 20, Springer Nature, 2024, pp. 310–21, doi:<a href="https://doi.org/10.1038/s41567-023-02302-1">10.1038/s41567-023-02302-1</a>.
  short: S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I.
    Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez,
    C.-P.J. Heisenberg, Nature Physics 20 (2024) 310–321.
corr_author: '1'
date_created: 2024-01-21T23:00:57Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2025-09-04T11:48:28Z
day: '01'
ddc:
- '530'
department:
- _id: CaHe
- _id: JoFi
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
doi: 10.1038/s41567-023-02302-1
external_id:
  isi:
  - '001138880800005'
  pmid:
  - '38370025'
file:
- access_level: open_access
  checksum: 7891ebe7c900ae47469ab127031dd1ec
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  creator: dernst
  date_created: 2024-07-16T12:12:43Z
  date_updated: 2024-07-16T12:12:43Z
  file_id: '17267'
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  file_size: 9897883
  relation: main_file
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file_date_updated: 2024-07-16T12:12:43Z
fulldoi: https://doi.org/10.1038/s41567-023-02302-1
has_accepted_license: '1'
intvolume: '        20'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 310-321
pmid: 1
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03601
  name: Control of embryonic cleavage pattern
publication: Nature Physics
publication_identifier:
  eissn:
  - 1745-2481
  issn:
  - 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on ISTA Website
    relation: press_release
    url: https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/
scopus_import: '1'
status: public
title: Friction forces determine cytoplasmic reorganization and shape changes of ascidian
  oocytes upon fertilization
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 20
year: '2024'
...
---
_id: '15264'
abstract:
- lang: eng
  text: Signaling by the B cell antigen receptor (BCR) initiates actin remodeling.
    The assembly of branched actin networks that are nucleated by the Arp2/3 complex
    exert outward force on the plasma membrane, allowing B cells to form membrane
    protrusions that can scan the surface of antigen-presenting cells (APCs). The
    resulting Arp2/3 complex-dependent actin retrograde flow promotes the centripetal
    movement and progressive coalescence of BCR microclusters, which amplifies BCR
    signaling. Glia maturation factor γ (GMFγ) is an actin disassembly-protein that
    releases Arp2/3 complex-nucleated actin filaments from actin networks. By doing
    so, GMFγ could either oppose the actions of the Arp2/3 complex or support Arp2/3
    complex-nucleated actin polymerization by contributing to the recycling of actin
    monomers and Arp2/3 complexes. We now show that reducing the levels of GMFγ in
    human B cell lines via transfection with a specific siRNA impairs the ability
    of B cells to spread on antigen-coated surfaces, decreases the velocity of actin
    retrograde flow, diminishes the coalescence of BCR microclusters into a central
    cluster at the B cell-APC contact site, and decreases APC-induced BCR signaling.
    These effects of depleting GMFγ are similar to what occurs when the Arp2/3 complex
    is inhibited. This suggests that GMFγ cooperates with the Arp2/3 complex to support
    BCR-induced actin remodeling and amplify BCR signaling at the immune synapse.
article_number: '647063'
article_processing_charge: No
article_type: original
author:
- first_name: Nikola
  full_name: Deretic, Nikola
  last_name: Deretic
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Kate
  full_name: Choi, Kate
  last_name: Choi
- first_name: Libin
  full_name: Abraham, Libin
  last_name: Abraham
- first_name: Michael R.
  full_name: Gold, Michael R.
  last_name: Gold
citation:
  ama: Deretic N, Bolger-Munro M, Choi K, Abraham L, Gold MR. The actin-disassembly
    protein glia maturation factor γ enhances actin remodeling and B cell antigen
    receptor signaling at the immune synapse. <i>Frontiers in Cell and Developmental
    Biology</i>. 2021;9. doi:<a href="https://doi.org/10.3389/fcell.2021.647063">10.3389/fcell.2021.647063</a>
  apa: Deretic, N., Bolger-Munro, M., Choi, K., Abraham, L., &#38; Gold, M. R. (2021).
    The actin-disassembly protein glia maturation factor γ enhances actin remodeling
    and B cell antigen receptor signaling at the immune synapse. <i>Frontiers in Cell
    and Developmental Biology</i>. Frontiers Media. <a href="https://doi.org/10.3389/fcell.2021.647063">https://doi.org/10.3389/fcell.2021.647063</a>
  chicago: Deretic, Nikola, Madison Bolger-Munro, Kate Choi, Libin Abraham, and Michael
    R. Gold. “The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin
    Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.” <i>Frontiers
    in Cell and Developmental Biology</i>. Frontiers Media, 2021. <a href="https://doi.org/10.3389/fcell.2021.647063">https://doi.org/10.3389/fcell.2021.647063</a>.
  ieee: N. Deretic, M. Bolger-Munro, K. Choi, L. Abraham, and M. R. Gold, “The actin-disassembly
    protein glia maturation factor γ enhances actin remodeling and B cell antigen
    receptor signaling at the immune synapse,” <i>Frontiers in Cell and Developmental
    Biology</i>, vol. 9. Frontiers Media, 2021.
  ista: Deretic N, Bolger-Munro M, Choi K, Abraham L, Gold MR. 2021. The actin-disassembly
    protein glia maturation factor γ enhances actin remodeling and B cell antigen
    receptor signaling at the immune synapse. Frontiers in Cell and Developmental
    Biology. 9, 647063.
  mla: Deretic, Nikola, et al. “The Actin-Disassembly Protein Glia Maturation Factor
    γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune
    Synapse.” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9, 647063,
    Frontiers Media, 2021, doi:<a href="https://doi.org/10.3389/fcell.2021.647063">10.3389/fcell.2021.647063</a>.
  short: N. Deretic, M. Bolger-Munro, K. Choi, L. Abraham, M.R. Gold, Frontiers in
    Cell and Developmental Biology 9 (2021).
date_created: 2024-04-03T07:34:08Z
date_published: 2021-07-01T00:00:00Z
date_updated: 2024-04-03T14:10:25Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.3389/fcell.2021.647063
external_id:
  pmid:
  - '34336818'
file:
- access_level: open_access
  checksum: f6330b5c6718d6780383c0300fd4ef12
  content_type: application/pdf
  creator: dernst
  date_created: 2024-04-03T14:08:05Z
  date_updated: 2024-04-03T14:08:05Z
  file_id: '15291'
  file_name: 2021_Frontiers_Deretic.pdf
  file_size: 7430029
  relation: main_file
  success: 1
file_date_updated: 2024-04-03T14:08:05Z
fulldoi: https://doi.org/10.3389/fcell.2021.647063
has_accepted_license: '1'
intvolume: '         9'
keyword:
- Cell Biology
- Developmental Biology
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Cell and Developmental Biology
publication_identifier:
  issn:
  - 2296-634X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: The actin-disassembly protein glia maturation factor γ enhances actin remodeling
  and B cell antigen receptor signaling at the immune synapse
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2021'
...
---
_id: '9379'
abstract:
- lang: eng
  text: When B cells encounter membrane-bound antigens, the formation and coalescence
    of B cell antigen receptor (BCR) microclusters amplifies BCR signaling. The ability
    of B cells to probe the surface of antigen-presenting cells (APCs) and respond
    to APC-bound antigens requires remodeling of the actin cytoskeleton. Initial BCR
    signaling stimulates actin-related protein (Arp) 2/3 complex-dependent actin polymerization,
    which drives B cell spreading as well as the centripetal movement and coalescence
    of BCR microclusters at the B cell-APC synapse. Sustained actin polymerization
    depends on concomitant actin filament depolymerization, which enables the recycling
    of actin monomers and Arp2/3 complexes. Cofilin-mediated severing of actin filaments
    is a rate-limiting step in the morphological changes that occur during immune
    synapse formation. Hence, regulators of cofilin activity such as WD repeat-containing
    protein 1 (Wdr1), LIM domain kinase (LIMK), and coactosin-like 1 (Cotl1) may also
    be essential for actin-dependent processes in B cells. Wdr1 enhances cofilin-mediated
    actin disassembly. Conversely, Cotl1 competes with cofilin for binding to actin
    and LIMK phosphorylates cofilin and prevents it from binding to actin filaments.
    We now show that Wdr1 and LIMK have distinct roles in BCR-induced assembly of
    the peripheral actin structures that drive B cell spreading, and that cofilin,
    Wdr1, and LIMK all contribute to the actin-dependent amplification of BCR signaling
    at the immune synapse. Depleting Cotl1 had no effect on these processes. Thus,
    the Wdr1-LIMK-cofilin axis is critical for BCR-induced actin remodeling and for
    B cell responses to APC-bound antigens.
acknowledgement: We thank the UBC Life Sciences Institute Imaging Facility andthe
  UBC Flow Cytometry Facility.
article_number: '649433'
article_processing_charge: No
article_type: original
author:
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Kate
  full_name: Choi, Kate
  last_name: Choi
- first_name: Faith
  full_name: Cheung, Faith
  last_name: Cheung
- first_name: Yi Tian
  full_name: Liu, Yi Tian
  last_name: Liu
- first_name: May
  full_name: Dang-Lawson, May
  last_name: Dang-Lawson
- first_name: Nikola
  full_name: Deretic, Nikola
  last_name: Deretic
- first_name: Connor
  full_name: Keane, Connor
  last_name: Keane
- first_name: Michael R.
  full_name: Gold, Michael R.
  last_name: Gold
citation:
  ama: Bolger-Munro M, Choi K, Cheung F, et al. The Wdr1-LIMK-Cofilin axis controls
    B cell antigen receptor-induced actin remodeling and signaling at the immune synapse.
    <i>Frontiers in Cell and Developmental Biology</i>. 2021;9. doi:<a href="https://doi.org/10.3389/fcell.2021.649433">10.3389/fcell.2021.649433</a>
  apa: Bolger-Munro, M., Choi, K., Cheung, F., Liu, Y. T., Dang-Lawson, M., Deretic,
    N., … Gold, M. R. (2021). The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced
    actin remodeling and signaling at the immune synapse. <i>Frontiers in Cell and
    Developmental Biology</i>. Frontiers Media. <a href="https://doi.org/10.3389/fcell.2021.649433">https://doi.org/10.3389/fcell.2021.649433</a>
  chicago: Bolger-Munro, Madison, Kate Choi, Faith Cheung, Yi Tian Liu, May Dang-Lawson,
    Nikola Deretic, Connor Keane, and Michael R. Gold. “The Wdr1-LIMK-Cofilin Axis
    Controls B Cell Antigen Receptor-Induced Actin Remodeling and Signaling at the
    Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>. Frontiers
    Media, 2021. <a href="https://doi.org/10.3389/fcell.2021.649433">https://doi.org/10.3389/fcell.2021.649433</a>.
  ieee: M. Bolger-Munro <i>et al.</i>, “The Wdr1-LIMK-Cofilin axis controls B cell
    antigen receptor-induced actin remodeling and signaling at the immune synapse,”
    <i>Frontiers in Cell and Developmental Biology</i>, vol. 9. Frontiers Media, 2021.
  ista: Bolger-Munro M, Choi K, Cheung F, Liu YT, Dang-Lawson M, Deretic N, Keane
    C, Gold MR. 2021. The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced
    actin remodeling and signaling at the immune synapse. Frontiers in Cell and Developmental
    Biology. 9, 649433.
  mla: Bolger-Munro, Madison, et al. “The Wdr1-LIMK-Cofilin Axis Controls B Cell Antigen
    Receptor-Induced Actin Remodeling and Signaling at the Immune Synapse.” <i>Frontiers
    in Cell and Developmental Biology</i>, vol. 9, 649433, Frontiers Media, 2021,
    doi:<a href="https://doi.org/10.3389/fcell.2021.649433">10.3389/fcell.2021.649433</a>.
  short: M. Bolger-Munro, K. Choi, F. Cheung, Y.T. Liu, M. Dang-Lawson, N. Deretic,
    C. Keane, M.R. Gold, Frontiers in Cell and Developmental Biology 9 (2021).
date_created: 2021-05-09T22:01:37Z
date_published: 2021-04-13T00:00:00Z
date_updated: 2023-10-18T08:19:49Z
day: '13'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.3389/fcell.2021.649433
external_id:
  isi:
  - '000644419500001'
  pmid:
  - '33928084'
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fulldoi: https://doi.org/10.3389/fcell.2021.649433
has_accepted_license: '1'
intvolume: '         9'
isi: 1
keyword:
- B cell
- actin
- immune synapse
- cell spreading
- cofilin
- WDR1 (AIP1)
- LIM domain kinase
- B cell receptor (BCR)
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Cell and Developmental Biology
publication_identifier:
  eissn:
  - 2296-634X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced actin remodeling
  and signaling at the immune synapse
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2021'
...