[{"publication_status":"submitted","language":[{"iso":"eng"}],"date_published":"2026-02-16T00:00:00Z","_id":"21291","corr_author":"1","acknowledgement":"We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko, and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB consortium for comments on\r\nthe manuscript. We are also grateful for the technical support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA). In addition, we thank our funding sources for providing\r\nthe resources to do these experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.); Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C. by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S. and G.C. were\r\nadditionally supported by institutional funds from the ISTA and the University of Exeter,\r\nrespectively. ","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"grant_number":"F7814","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e"},{"_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression","grant_number":"F7805"}],"abstract":[{"text":"The complexity and specificity of movement in vertebrates is driven by a rich diversity of spinal motor and interneuron cell types. During development, eleven spinal cord progenitor domains generate an equivalent number of cardinal neuron types. How progenitor domains, individual progenitors, and post-mitotic diversity relate is still unknown. We performed high-resolution, single-progenitor cell lineage tracing in the embryonic mouse spinal cord using mosaic analysis with double markers (MADM). Our quantitative study of lineage progression revealed that spinal cord progenitors undergo highly variable numbers of proliferative, neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons migrate radially over large distances, span the dorsoventral axis, and even cross the midline, demonstrating striking bilaterality. Molecular and morphometric analysis indicate high levels of progenitor multipotency, with an individual progenitor capable of producing several molecularly and morphologically distinct neuron types, as well as astrocytes. These findings redefine spinal cord development as a process in which lineage variability — rather than rigid progenitor identity — drives the generation of cellular diversity.","lang":"eng"}],"type":"preprint","month":"02","main_file_link":[{"url":"https://doi.org/10.64898/2026.02.12.705305","open_access":"1"}],"article_processing_charge":"No","oa_version":"Preprint","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","date_created":"2026-02-17T11:36:20Z","doi":"10.64898/2026.02.12.705305","title":"Lineage origin of spinal cord cell type diversity","department":[{"_id":"SiHi"},{"_id":"LoSw"}],"ddc":["570"],"OA_type":"green","citation":{"apa":"Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher, C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>","ieee":"S. A. Gobeil <i>et al.</i>, “Lineage origin of spinal cord cell type diversity,” <i>bioRxiv</i>. .","mla":"Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>.","ama":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>","ista":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity. bioRxiv, <a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>.","chicago":"Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B. Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>.","short":"S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher, G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.)."},"publication":"bioRxiv","status":"public","has_accepted_license":"1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"date_updated":"2026-04-14T08:16:55Z","year":"2026","author":[{"first_name":"Sophie A","last_name":"Gobeil","full_name":"Gobeil, Sophie A","id":"2f3e9efb-eb24-11ec-86b2-88efb11d59fa"},{"id":"8cfb7412-10a7-11f1-add1-82b44e6418f2","full_name":"Da Silveira Neto, Francisco","last_name":"Da Silveira Neto","first_name":"Francisco"},{"id":"12632ae8-799e-11ef-94a2-e5a3b5ef49e9","full_name":"Silvestrelli, Giulia","last_name":"Silvestrelli","first_name":"Giulia"},{"full_name":"Smits, Matthijs Geert","last_name":"Smits","first_name":"Matthijs Geert","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0"},{"first_name":"Carmen","last_name":"Streicher","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87"},{"id":"471195F6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8457-2572","first_name":"Giselle T","last_name":"Cheung","full_name":"Cheung, Giselle T"},{"orcid":"0000-0003-2279-1061","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","first_name":"Simon","full_name":"Hippenmeyer, Simon"},{"full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"repository","day":"16"},{"publisher":"Elsevier","file":[{"creator":"dernst","file_name":"2025_DevelopmentalCell_Jaeger.pdf","relation":"main_file","file_id":"19790","date_updated":"2025-06-04T05:43:27Z","access_level":"open_access","date_created":"2025-06-04T05:43:27Z","success":1,"file_size":11936258,"checksum":"a83a4cb58f5941096d3ad91ca0172594","content_type":"application/pdf"}],"has_accepted_license":"1","scopus_import":"1","page":"794-812.e6","OA_type":"hybrid","citation":{"chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>.","ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6.","mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>, vol. 60, no. 5, Elsevier, 2025, p. 794–812.e6, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>.","apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>","ieee":"E. C. B. Jaeger <i>et al.</i>, “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” <i>Developmental Cell</i>, vol. 60, no. 5. Elsevier, p. 794–812.e6, 2025.","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. 2025;60(5):794-812.e6. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>"},"ddc":["570"],"status":"public","publication":"Developmental Cell","author":[{"full_name":"Jaeger, Eliza C.B.","last_name":"Jaeger","first_name":"Eliza C.B."},{"last_name":"Vijatovic","first_name":"David","full_name":"Vijatovic, David","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"full_name":"Deryckere, Astrid","last_name":"Deryckere","first_name":"Astrid"},{"last_name":"Zorin","first_name":"Nikol","full_name":"Zorin, Nikol"},{"full_name":"Nguyen, Akemi L.","last_name":"Nguyen","first_name":"Akemi L."},{"id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","full_name":"Ivanian, Georgiy","first_name":"Georgiy","last_name":"Ivanian"},{"last_name":"Woych","first_name":"Jamie","full_name":"Woych, Jamie"},{"full_name":"Arnold, Rebecca C","first_name":"Rebecca C","last_name":"Arnold","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f"},{"last_name":"Ortega Gurrola","first_name":"Alonso","full_name":"Ortega Gurrola, Alonso"},{"full_name":"Shvartsman, Arik","last_name":"Shvartsman","first_name":"Arik"},{"id":"a9492887-8972-11ed-ae7b-bfae10998254","first_name":"Francesca","last_name":"Barbieri","full_name":"Barbieri, Francesca"},{"full_name":"Toma, Florina-Alexandra","last_name":"Toma","first_name":"Florina-Alexandra","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5"},{"full_name":"Gorbsky, Gary J.","first_name":"Gary J.","last_name":"Gorbsky"},{"first_name":"Marko E.","last_name":"Horb","full_name":"Horb, Marko E."},{"full_name":"Cline, Hollis T.","first_name":"Hollis T.","last_name":"Cline"},{"full_name":"Shay, Timothy F.","first_name":"Timothy F.","last_name":"Shay"},{"full_name":"Kelley, Darcy B.","last_name":"Kelley","first_name":"Darcy B."},{"full_name":"Yamaguchi, Ayako","last_name":"Yamaguchi","first_name":"Ayako"},{"full_name":"Shein-Idelson, Mark","first_name":"Mark","last_name":"Shein-Idelson"},{"full_name":"Tosches, Maria Antonietta","first_name":"Maria Antonietta","last_name":"Tosches"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger"}],"year":"2025","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"date_updated":"2025-09-30T10:00:55Z","oa":1,"article_type":"original","quality_controlled":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"OA_place":"publisher","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","day":"10","issue":"5","volume":60,"language":[{"iso":"eng"}],"pmid":1,"intvolume":"        60","publication_status":"published","isi":1,"_id":"15016","date_published":"2025-03-10T00:00:00Z","external_id":{"pmid":["39603234"],"isi":["001444798600001"]},"project":[{"grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a"},{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814"}],"type":"journal_article","file_date_updated":"2025-06-04T05:43:27Z","month":"03","abstract":[{"text":"Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although successful in mammals, AAVs have never been shown to transduce amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and identified at least two AAV serotypes per species that transduce neurons. In developing amphibians, AAVs labeled groups of neurons generated at the same time during development. In the mature brain, AAVrg retrogradely traced long-range projections. Our study introduces AAVs as a tool for amphibian research, establishes a generalizable workflow for AAV screening in new species, and expands opportunities for cross-species comparisons of nervous system development, function, and evolution.","lang":"eng"}],"acknowledgement":"We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion, and support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru (Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination; our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC, and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.); FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.); EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).","corr_author":"1","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"oa_version":"Published Version","date_created":"2024-02-20T09:20:32Z","doi":"10.1016/j.devcel.2024.10.025","article_processing_charge":"Yes (via OA deal)","title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians"},{"department":[{"_id":"LoSw"}],"OA_embargo":"6 months","title":"Molecular and cellular mechanisms of motor circuit development","main_file_link":[{"url":"https://doi.org/10.1523/JNEUROSCI.1238-24.2024","open_access":"1"}],"article_processing_charge":"No","doi":"10.1523/JNEUROSCI.1238-24.2024","oa_version":"Published Version","date_created":"2024-10-13T22:01:49Z","abstract":[{"lang":"eng","text":"Motor circuits represent the main output of the central nervous system and produce dynamic behaviors ranging from relatively simple rhythmic activities like swimming in fish and breathing in mammals to highly sophisticated dexterous movements in humans. Despite decades of research, the development and function of motor circuits remain poorly understood. Breakthroughs in the field recently provided new tools and tractable model systems that set the stage to discover the molecular mechanisms and circuit logic underlying motor control. Here, we describe recent advances from both vertebrate (mouse, frog) and invertebrate (nematode, fruit fly) systems on cellular and molecular mechanisms that enable motor circuits to develop and function and highlight conserved and divergent mechanisms necessary for motor circuit development."}],"type":"journal_article","month":"10","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"}],"external_id":{"isi":["001335212200016"],"pmid":["39358025"]},"publication_identifier":{"eissn":["1529-2401"]},"acknowledgement":"Work in the authors’ labs is funded by the Helmholtz Association (N.Z.), National Institute of Neurological Disorders and Stroke (NINDS) R01NS116365 (P.K.), NINDS R01NS123439 and National Science Foundation IOS-2048080 (R.C.), NINDS R01NS114510 (P.P.), Natural Sciences and Engineering Research Council of Canada RGPIN-2021-03154 (K.M.) and Horizon Europe European Research Council Starting Grant Number 101041551 (L.B.S.). P.P. is the Weidenthal Family Designated Professor in Career Development.","_id":"18305","isi":1,"date_published":"2024-10-02T00:00:00Z","language":[{"iso":"eng"}],"publication_status":"published","intvolume":"        44","pmid":1,"day":"02","OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":44,"issue":"40","quality_controlled":"1","article_type":"original","oa":1,"year":"2024","author":[{"full_name":"Kratsios, Paschalis","first_name":"Paschalis","last_name":"Kratsios"},{"first_name":"Niccolò","last_name":"Zampieri","full_name":"Zampieri, Niccolò"},{"full_name":"Carrillo, Robert","first_name":"Robert","last_name":"Carrillo"},{"first_name":"Kota","last_name":"Mizumoto","full_name":"Mizumoto, Kota"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger"},{"full_name":"Philippidou, Polyxeni","last_name":"Philippidou","first_name":"Polyxeni"}],"date_updated":"2026-01-05T14:01:26Z","scopus_import":"1","has_accepted_license":"1","publisher":"Society for Neuroscience","publication":"The Journal of Neuroscience","status":"public","ddc":["570"],"citation":{"short":"P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L.B. Sweeney, P. Philippidou, The Journal of Neuroscience 44 (2024).","ista":"Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P. 2024. Molecular and cellular mechanisms of motor circuit development. The Journal of Neuroscience. 44(40), e1238242024.","chicago":"Kratsios, Paschalis, Niccolò Zampieri, Robert Carrillo, Kota Mizumoto, Lora B. Sweeney, and Polyxeni Philippidou. “Molecular and Cellular Mechanisms of Motor Circuit Development.” <i>The Journal of Neuroscience</i>. Society for Neuroscience, 2024. <a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">https://doi.org/10.1523/JNEUROSCI.1238-24.2024</a>.","ieee":"P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L. B. Sweeney, and P. Philippidou, “Molecular and cellular mechanisms of motor circuit development,” <i>The Journal of Neuroscience</i>, vol. 44, no. 40. Society for Neuroscience, 2024.","ama":"Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P. Molecular and cellular mechanisms of motor circuit development. <i>The Journal of Neuroscience</i>. 2024;44(40). doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">10.1523/JNEUROSCI.1238-24.2024</a>","apa":"Kratsios, P., Zampieri, N., Carrillo, R., Mizumoto, K., Sweeney, L. B., &#38; Philippidou, P. (2024). Molecular and cellular mechanisms of motor circuit development. <i>The Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">https://doi.org/10.1523/JNEUROSCI.1238-24.2024</a>","mla":"Kratsios, Paschalis, et al. “Molecular and Cellular Mechanisms of Motor Circuit Development.” <i>The Journal of Neuroscience</i>, vol. 44, no. 40, e1238242024, Society for Neuroscience, 2024, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">10.1523/JNEUROSCI.1238-24.2024</a>."},"article_number":"e1238242024","OA_type":"hybrid"},{"day":"27","OA_place":"repository","department":[{"_id":"LoSw"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.09.20.614050"}],"article_processing_charge":"No","oa_version":"Preprint","doi":"10.1101/2024.09.20.614050","date_created":"2025-04-07T08:48:28Z","abstract":[{"lang":"eng","text":"Vertebrates exhibit a wide range of motor behaviors, ranging from swimming to complex limb-based movements. Here we take advantage of frog metamorphosis, which captures a swim-to-limb-based movement transformation during the development of a single organism, to explore changes in the underlying spinal circuits. We find that the tadpole spinal cord contains small and largely homogeneous populations of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages. These neuronal populations only modestly increase in number and subtype heterogeneity with the emergence of free swimming. In contrast, during frog metamorphosis and the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron number and transcriptional heterogeneity, culminating in cohorts of neurons that exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively, results in severe but selective deficits in tail and limb function. Our work thus demonstrates that neural diversity scales exponentially with increasing behavioral complexity and illustrates striking evolutionary conservation in the molecular organization and function of motor circuits across species."}],"type":"preprint","month":"09","project":[{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a"},{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits"},{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy","grant_number":"CZI01"}],"oa":1,"corr_author":"1","acknowledgement":"We would like to thank the members of the Sweeney Lab (especially Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion, and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their scientific insight and mentorship during the conception of this project. This\r\nproject would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA). In addition, we thank our funding sources for providing the resources to do these\r\nexperiments: FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS 5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.); NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities (ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472 (Z.H.); and Project A.L.S. (S.B-M.). ","_id":"19520","year":"2024","author":[{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","full_name":"Vijatovic, David","first_name":"David","last_name":"Vijatovic"},{"id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","first_name":"Florina Alexandra ","last_name":"Toma","full_name":"Toma, Florina Alexandra "},{"first_name":"Zoe P","last_name":"Harrington","full_name":"Harrington, Zoe P","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","orcid":"0009-0008-0158-4032"},{"last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Trevisan","first_name":"Alexandra J.","full_name":"Trevisan, Alexandra J."},{"full_name":"Chapman, Phillip","last_name":"Chapman","first_name":"Phillip"},{"full_name":"Julseth, Mara","last_name":"Julseth","first_name":"Mara","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1"},{"full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton","first_name":"Susan"},{"first_name":"Mariano I.","last_name":"Gabitto","full_name":"Gabitto, Mariano I."},{"full_name":"Dasen, Jeremy S.","first_name":"Jeremy S.","last_name":"Dasen"},{"full_name":"Bikoff, Jay B.","last_name":"Bikoff","first_name":"Jay B."},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger"}],"date_updated":"2025-05-14T11:40:13Z","date_published":"2024-09-27T00:00:00Z","acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"publication_status":"submitted","publication":"bioRxiv","status":"public","citation":{"chicago":"Vijatovic, David, Florina Alexandra  Toma, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>.","short":"D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).","ista":"Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv, <a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","ieee":"D. Vijatovic <i>et al.</i>, “Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis,” <i>bioRxiv</i>. .","mla":"Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","apa":"Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R., Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>","ama":"Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>"},"OA_type":"green"},{"department":[{"_id":"LoSw"}],"title":"Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges","article_processing_charge":"Yes (in subscription journal)","oa_version":"Published Version","doi":"10.1073/pnas.2318041121","date_created":"2024-04-21T22:00:53Z","abstract":[{"lang":"eng","text":"Stable matching of neurotransmitters with their receptors is fundamental to synapse function and reliable communication in neural circuits. Presynaptic neurotransmitters regulate the stabilization of postsynaptic transmitter receptors. Whether postsynaptic receptors regulate stabilization of presynaptic transmitters has received less attention. Here, we show that blockade of endogenous postsynaptic acetylcholine receptors (AChR) at the neuromuscular junction destabilizes the cholinergic phenotype in motor neurons and stabilizes an earlier, developmentally transient glutamatergic phenotype. Further, expression of exogenous postsynaptic gamma-aminobutyric acid type A receptors (GABAA receptors) in muscle cells stabilizes an earlier, developmentally transient GABAergic motor neuron phenotype. Both AChR and GABAA receptors are linked to presynaptic neurons through transsynaptic bridges. Knockdown of specific components of these transsynaptic bridges prevents stabilization of the cholinergic or GABAergic phenotypes. Bidirectional communication can enforce a match between transmitter and receptor and ensure the fidelity of synaptic transmission. Our findings suggest a potential role of dysfunctional transmitter receptors in neurological disorders that involve the loss of the presynaptic transmitter."}],"type":"journal_article","file_date_updated":"2024-04-23T06:53:14Z","month":"04","external_id":{"isi":["001243892800004"],"pmid":["38568976"]},"publication_identifier":{"eissn":["1091-6490"]},"acknowledgement":"We  thank  all  members  of  the  Spitzer  laboratory  for  discussions  and  critical  feedback;  K.  Marek  for  discussions  of  acknowledgment  signals; I. Gregor and R. Aricescu for discussions of receptor pharmacology and transsynaptic  bridges;  C.  Kintner  for  advice  on  Xenopus  blastomere  lineage;  A.  Ray and E. Park for guidance on miniature analysis; A. Glavis- Bloom, S.U. Choi, S. Atkins, M. Gupta, and S. Malladi for technical assistance; and D. K. Berg and L. R. Squire for comments on the manuscript. This work was supported by NSF 2051555 and the Overland Foundation. Microscopy for five- channel imaging utilized the UCSD School of Medicine Microscopy Core, supported by NIH grant NS047101.","isi":1,"_id":"15335","date_published":"2024-04-09T00:00:00Z","language":[{"iso":"eng"}],"publication_status":"published","intvolume":"       121","pmid":1,"day":"09","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","volume":121,"issue":"15","quality_controlled":"1","article_type":"original","oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"year":"2024","author":[{"last_name":"Godavarthi","first_name":"Swetha K.","full_name":"Godavarthi, Swetha K."},{"full_name":"Hiramoto, Masaki","last_name":"Hiramoto","first_name":"Masaki"},{"full_name":"Ignatyev, Yuri","last_name":"Ignatyev","first_name":"Yuri"},{"full_name":"Levin, Jacqueline B.","last_name":"Levin","first_name":"Jacqueline B."},{"full_name":"Li, Hui Quan","last_name":"Li","first_name":"Hui Quan"},{"full_name":"Pratelli, Marta","last_name":"Pratelli","first_name":"Marta"},{"first_name":"Jennifer","last_name":"Borchardt","full_name":"Borchardt, Jennifer"},{"full_name":"Czajkowski, Cynthia","first_name":"Cynthia","last_name":"Czajkowski"},{"last_name":"Borodinsky","first_name":"Laura N.","full_name":"Borodinsky, Laura N."},{"orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger"},{"first_name":"Hollis T.","last_name":"Cline","full_name":"Cline, Hollis T."},{"last_name":"Spitzer","first_name":"Nicholas C.","full_name":"Spitzer, Nicholas C."}],"date_updated":"2025-09-04T13:42:01Z","has_accepted_license":"1","scopus_import":"1","file":[{"file_name":"2024_PNAS_Godavarthi.pdf","creator":"dernst","file_id":"15340","relation":"main_file","file_size":16187094,"checksum":"f3b4ffad4ef3d1c443414edf0cd2392c","content_type":"application/pdf","date_updated":"2024-04-23T06:53:14Z","access_level":"open_access","date_created":"2024-04-23T06:53:14Z","success":1}],"publisher":"National Academy of Sciences","publication":"Proceedings of the National Academy of Sciences of the United States of America","status":"public","citation":{"mla":"Godavarthi, Swetha K., et al. “Postsynaptic Receptors Regulate Presynaptic Transmitter Stability through Transsynaptic Bridges.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 15, e2318041121, National Academy of Sciences, 2024, doi:<a href=\"https://doi.org/10.1073/pnas.2318041121\">10.1073/pnas.2318041121</a>.","ama":"Godavarthi SK, Hiramoto M, Ignatyev Y, et al. Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2024;121(15). doi:<a href=\"https://doi.org/10.1073/pnas.2318041121\">10.1073/pnas.2318041121</a>","ieee":"S. K. Godavarthi <i>et al.</i>, “Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 15. National Academy of Sciences, 2024.","apa":"Godavarthi, S. K., Hiramoto, M., Ignatyev, Y., Levin, J. B., Li, H. Q., Pratelli, M., … Spitzer, N. C. (2024). Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2318041121\">https://doi.org/10.1073/pnas.2318041121</a>","short":"S.K. Godavarthi, M. Hiramoto, Y. Ignatyev, J.B. Levin, H.Q. Li, M. Pratelli, J. Borchardt, C. Czajkowski, L.N. Borodinsky, L.B. Sweeney, H.T. Cline, N.C. Spitzer, Proceedings of the National Academy of Sciences of the United States of America 121 (2024).","chicago":"Godavarthi, Swetha K., Masaki Hiramoto, Yuri Ignatyev, Jacqueline B. Levin, Hui Quan Li, Marta Pratelli, Jennifer Borchardt, et al. “Postsynaptic Receptors Regulate Presynaptic Transmitter Stability through Transsynaptic Bridges.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2024. <a href=\"https://doi.org/10.1073/pnas.2318041121\">https://doi.org/10.1073/pnas.2318041121</a>.","ista":"Godavarthi SK, Hiramoto M, Ignatyev Y, Levin JB, Li HQ, Pratelli M, Borchardt J, Czajkowski C, Borodinsky LN, Sweeney LB, Cline HT, Spitzer NC. 2024. Postsynaptic receptors regulate presynaptic transmitter stability through transsynaptic bridges. Proceedings of the National Academy of Sciences of the United States of America. 121(15), e2318041121."},"ddc":["570"],"article_number":"e2318041121"},{"year":"2023","author":[{"orcid":"0000-0001-6191-1367","id":"5230e794-15b2-11ec-abd3-e2d5335ebd1d","last_name":"Wilson","first_name":"Alexia C","full_name":"Wilson, Alexia C"},{"first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"date_updated":"2026-04-07T12:36:07Z","has_accepted_license":"1","scopus_import":"1","publisher":"Frontiers","file":[{"date_updated":"2024-01-03T13:33:21Z","access_level":"open_access","date_created":"2024-01-03T13:33:21Z","success":1,"content_type":"application/pdf","checksum":"7efd06de284a28e91e97127611a9c3fd","file_size":6667157,"creator":"dernst","file_name":"2023_FrontiersNeuralCircuits_Wilson.pdf","relation":"main_file","file_id":"14729"}],"ddc":["570"],"article_number":"1146449","citation":{"ieee":"A. C. Wilson and L. B. Sweeney, “Spinal cords: Symphonies of interneurons across species,” <i>Frontiers in Neural Circuits</i>, vol. 17. Frontiers, 2023.","ama":"Wilson AC, Sweeney LB. Spinal cords: Symphonies of interneurons across species. <i>Frontiers in Neural Circuits</i>. 2023;17. doi:<a href=\"https://doi.org/10.3389/fncir.2023.1146449\">10.3389/fncir.2023.1146449</a>","mla":"Wilson, Alexia C., and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” <i>Frontiers in Neural Circuits</i>, vol. 17, 1146449, Frontiers, 2023, doi:<a href=\"https://doi.org/10.3389/fncir.2023.1146449\">10.3389/fncir.2023.1146449</a>.","apa":"Wilson, A. C., &#38; Sweeney, L. B. (2023). Spinal cords: Symphonies of interneurons across species. <i>Frontiers in Neural Circuits</i>. Frontiers. <a href=\"https://doi.org/10.3389/fncir.2023.1146449\">https://doi.org/10.3389/fncir.2023.1146449</a>","short":"A.C. Wilson, L.B. Sweeney, Frontiers in Neural Circuits 17 (2023).","chicago":"Wilson, Alexia C, and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” <i>Frontiers in Neural Circuits</i>. Frontiers, 2023. <a href=\"https://doi.org/10.3389/fncir.2023.1146449\">https://doi.org/10.3389/fncir.2023.1146449</a>.","ista":"Wilson AC, Sweeney LB. 2023. Spinal cords: Symphonies of interneurons across species. Frontiers in Neural Circuits. 17, 1146449."},"status":"public","publication":"Frontiers in Neural Circuits","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"26","volume":17,"oa":1,"quality_controlled":"1","article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"related_material":{"record":[{"relation":"dissertation_contains","id":"20735","status":"public"}]},"_id":"13097","isi":1,"date_published":"2023-04-26T00:00:00Z","language":[{"iso":"eng"}],"pmid":1,"publication_status":"published","intvolume":"        17","department":[{"_id":"LoSw"}],"article_processing_charge":"Yes","oa_version":"Published Version","doi":"10.3389/fncir.2023.1146449","date_created":"2023-05-28T22:01:04Z","title":"Spinal cords: Symphonies of interneurons across species","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"}],"external_id":{"pmid":["37180760"],"isi":["000984606200001"]},"abstract":[{"lang":"eng","text":"Vertebrate movement is orchestrated by spinal inter- and motor neurons that, together with sensory and cognitive input, produce dynamic motor behaviors. These behaviors vary from the simple undulatory swimming of fish and larval aquatic species to the highly coordinated running, reaching and grasping of mice, humans and other mammals. This variation raises the fundamental question of how spinal circuits have changed in register with motor behavior. In simple, undulatory fish, exemplified by the lamprey, two broad classes of interneurons shape motor neuron output: ipsilateral-projecting excitatory neurons, and commissural-projecting inhibitory neurons. An additional class of ipsilateral inhibitory neurons is required to generate escape swim behavior in larval zebrafish and tadpoles. In limbed vertebrates, a more complex spinal neuron composition is observed. In this review, we provide evidence that movement elaboration correlates with an increase and specialization of these three basic interneuron types into molecularly, anatomically, and functionally distinct subpopulations. We summarize recent work linking neuron types to movement-pattern generation across fish, amphibians, reptiles, birds and mammals."}],"file_date_updated":"2024-01-03T13:33:21Z","type":"journal_article","month":"04","corr_author":"1","acknowledgement":"This work was supported by the ERC Starting grant, ERC-2021-STG #101041551.","publication_identifier":{"issn":["1662-5110"]}},{"date_updated":"2024-10-09T21:00:14Z","author":[{"first_name":"Alina","last_name":"Salamatina","full_name":"Salamatina, Alina"},{"full_name":"Yang, Jerry H","last_name":"Yang","first_name":"Jerry H"},{"last_name":"Brenner-Morton","first_name":"Susan","full_name":"Brenner-Morton, Susan"},{"full_name":"Bikoff, Jay B ","last_name":"Bikoff","first_name":"Jay B "},{"full_name":"Fang, Linjing","last_name":"Fang","first_name":"Linjing"},{"full_name":"Kintner, Christopher R","last_name":"Kintner","first_name":"Christopher R"},{"full_name":"Jessell, Thomas M","last_name":"Jessell","first_name":"Thomas M"},{"full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601"}],"year":"2020","status":"public","publication":"Neuroscience","page":"81-95","citation":{"ama":"Salamatina A, Yang JH, Brenner-Morton S, et al. Differential loss of spinal interneurons in a mouse model of ALS. <i>Neuroscience</i>. 2020;450:81-95. doi:<a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">10.1016/j.neuroscience.2020.08.011</a>","ieee":"A. Salamatina <i>et al.</i>, “Differential loss of spinal interneurons in a mouse model of ALS,” <i>Neuroscience</i>, vol. 450. Elsevier, pp. 81–95, 2020.","apa":"Salamatina, A., Yang, J. H., Brenner-Morton, S., Bikoff, J. B., Fang, L., Kintner, C. R., … Sweeney, L. B. (2020). Differential loss of spinal interneurons in a mouse model of ALS. <i>Neuroscience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">https://doi.org/10.1016/j.neuroscience.2020.08.011</a>","mla":"Salamatina, Alina, et al. “Differential Loss of Spinal Interneurons in a Mouse Model of ALS.” <i>Neuroscience</i>, vol. 450, Elsevier, 2020, pp. 81–95, doi:<a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">10.1016/j.neuroscience.2020.08.011</a>.","chicago":"Salamatina, Alina, Jerry H Yang, Susan Brenner-Morton, Jay B  Bikoff, Linjing Fang, Christopher R Kintner, Thomas M Jessell, and Lora B. Sweeney. “Differential Loss of Spinal Interneurons in a Mouse Model of ALS.” <i>Neuroscience</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.neuroscience.2020.08.011\">https://doi.org/10.1016/j.neuroscience.2020.08.011</a>.","ista":"Salamatina A, Yang JH, Brenner-Morton S, Bikoff JB, Fang L, Kintner CR, Jessell TM, Sweeney LB. 2020. Differential loss of spinal interneurons in a mouse model of ALS. Neuroscience. 450, 81–95.","short":"A. Salamatina, J.H. Yang, S. Brenner-Morton, J.B. Bikoff, L. Fang, C.R. Kintner, T.M. Jessell, L.B. Sweeney, Neuroscience 450 (2020) 81–95."},"ddc":["570"],"publisher":"Elsevier","file":[{"file_size":4071247,"content_type":"application/pdf","checksum":"da7413c819e079720669c82451b49294","date_updated":"2020-12-03T11:45:26Z","access_level":"open_access","date_created":"2020-12-03T11:45:26Z","success":1,"file_name":"2020_Neuroscience_Salamatina.pdf","creator":"dernst","file_id":"8915","relation":"main_file"}],"scopus_import":"1","has_accepted_license":"1","volume":450,"day":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"article_type":"original","quality_controlled":"1","oa":1,"date_published":"2020-12-01T00:00:00Z","isi":1,"_id":"8914","intvolume":"       450","publication_status":"published","pmid":1,"language":[{"iso":"eng"}],"title":"Differential loss of spinal interneurons in a mouse model of ALS","date_created":"2020-12-03T11:47:31Z","oa_version":"Published Version","doi":"10.1016/j.neuroscience.2020.08.011","article_processing_charge":"Yes (via OA deal)","department":[{"_id":"LoSw"}],"publication_identifier":{"issn":["0306-4522"]},"acknowledgement":"This work was made possible by the generous support of Project ALS. Imaging and related analyses were facilitated by The Waitt Advanced Biophotonics Center Core at the Salk Institute, supported by grants from NIH-NCI CCSG (P30 014195) and NINDS Neuroscience Center (NS072031). The authors would like to additionally thank Drs. Jane Dodd, Robert Brownstone, and Laskaro Zagoraiou for helpful comments on the manuscript. This manuscript is dedicated to Tom Jessell, an inspirational scientist, friend and mentor.","corr_author":"1","type":"journal_article","month":"12","file_date_updated":"2020-12-03T11:45:26Z","abstract":[{"lang":"eng","text":"Amyotrophic lateral sclerosis (ALS) leads to a loss of specific motor neuron populations in the spinal cord and cortex. Emerging evidence suggests that interneurons may also be affected, but a detailed characterization of interneuron loss and its potential impacts on motor neuron loss and disease progression is lacking. To examine this issue, the fate of V1 inhibitory neurons during ALS was assessed in the ventral spinal cord using the SODG93A mouse model. The V1 population makes up ∼30% of all ventral inhibitory neurons, ∼50% of direct inhibitory synaptic contacts onto motor neuron cell bodies, and is thought to play a key role in modulating motor output, in part through recurrent and reciprocal inhibitory circuits. We find that approximately half of V1 inhibitory neurons are lost in SODG93A mice at late disease stages, but that this loss is delayed relative to the loss of motor neurons and V2a excitatory neurons. We further identify V1 subpopulations based on transcription factor expression that are differentially susceptible to degeneration in SODG93A mice. At an early disease stage, we show that V1 synaptic contacts with motor neuron cell bodies increase, suggesting an upregulation of inhibition before V1 neurons are lost in substantial numbers. These data support a model in which progressive changes in V1 synaptic contacts early in disease, and in select V1 subpopulations at later stages, represent a compensatory upregulation and then deleterious breakdown of specific interneuron circuits within the spinal cord."}],"external_id":{"pmid":["32858144"],"isi":["000595588700008"]}},{"publication_identifier":{"issn":["0896-6273"]},"abstract":[{"text":"Motor output varies along the rostro-caudal axis of the tetrapod spinal cord. At limb levels, ∼60 motor pools control the alternation of flexor and extensor muscles about each joint, whereas at thoracic levels as few as 10 motor pools supply muscle groups that support posture, inspiration, and expiration. Whether such differences in motor neuron identity and muscle number are associated with segmental distinctions in interneuron diversity has not been resolved. We show that select combinations of nineteen transcription factors that specify lumbar V1 inhibitory interneurons generate subpopulations enriched at limb and thoracic levels. Specification of limb and thoracic V1 interneurons involves the Hox gene Hoxc9 independently of motor neurons. Thus, early Hox patterning of the spinal cord determines the identity of V1 interneurons and motor neurons. These studies reveal a developmental program of V1 interneuron diversity, providing insight into the organization of inhibitory interneurons associated with differential motor output.","lang":"eng"}],"quality_controlled":"1","article_type":"original","month":"01","type":"journal_article","article_processing_charge":"No","issue":"2","date_created":"2020-04-30T10:35:13Z","doi":"10.1016/j.neuron.2017.12.029","oa_version":"None","volume":97,"title":"Origin and segmental diversity of spinal inhibitory interneurons","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"04","citation":{"chicago":"Sweeney, Lora B., Jay B. Bikoff, Mariano I. Gabitto, Susan Brenner-Morton, Myungin Baek, Jerry H. Yang, Esteban G. Tabak, Jeremy S. Dasen, Christopher R. Kintner, and Thomas M. Jessell. “Origin and Segmental Diversity of Spinal Inhibitory Interneurons.” <i>Neuron</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">https://doi.org/10.1016/j.neuron.2017.12.029</a>.","ista":"Sweeney LB, Bikoff JB, Gabitto MI, Brenner-Morton S, Baek M, Yang JH, Tabak EG, Dasen JS, Kintner CR, Jessell TM. 2018. Origin and segmental diversity of spinal inhibitory interneurons. Neuron. 97(2), 341–355.e3.","short":"L.B. Sweeney, J.B. Bikoff, M.I. Gabitto, S. Brenner-Morton, M. Baek, J.H. Yang, E.G. Tabak, J.S. Dasen, C.R. Kintner, T.M. Jessell, Neuron 97 (2018) 341–355.e3.","ieee":"L. B. Sweeney <i>et al.</i>, “Origin and segmental diversity of spinal inhibitory interneurons,” <i>Neuron</i>, vol. 97, no. 2. Elsevier, p. 341–355.e3, 2018.","mla":"Sweeney, Lora B., et al. “Origin and Segmental Diversity of Spinal Inhibitory Interneurons.” <i>Neuron</i>, vol. 97, no. 2, Elsevier, 2018, p. 341–355.e3, doi:<a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">10.1016/j.neuron.2017.12.029</a>.","ama":"Sweeney LB, Bikoff JB, Gabitto MI, et al. Origin and segmental diversity of spinal inhibitory interneurons. <i>Neuron</i>. 2018;97(2):341-355.e3. doi:<a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">10.1016/j.neuron.2017.12.029</a>","apa":"Sweeney, L. B., Bikoff, J. B., Gabitto, M. I., Brenner-Morton, S., Baek, M., Yang, J. H., … Jessell, T. M. (2018). Origin and segmental diversity of spinal inhibitory interneurons. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2017.12.029\">https://doi.org/10.1016/j.neuron.2017.12.029</a>"},"page":"341-355.e3","publication_status":"published","intvolume":"        97","status":"public","publication":"Neuron","language":[{"iso":"eng"}],"publisher":"Elsevier","date_published":"2018-01-04T00:00:00Z","extern":"1","date_updated":"2024-01-31T10:13:54Z","year":"2018","author":[{"last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"},{"first_name":"Jay B.","last_name":"Bikoff","full_name":"Bikoff, Jay B."},{"last_name":"Gabitto","first_name":"Mariano I.","full_name":"Gabitto, Mariano I."},{"full_name":"Brenner-Morton, Susan","first_name":"Susan","last_name":"Brenner-Morton"},{"full_name":"Baek, Myungin","last_name":"Baek","first_name":"Myungin"},{"full_name":"Yang, Jerry H.","last_name":"Yang","first_name":"Jerry H."},{"full_name":"Tabak, Esteban G.","last_name":"Tabak","first_name":"Esteban G."},{"full_name":"Dasen, Jeremy S.","last_name":"Dasen","first_name":"Jeremy S."},{"full_name":"Kintner, Christopher R.","first_name":"Christopher R.","last_name":"Kintner"},{"full_name":"Jessell, Thomas M.","first_name":"Thomas M.","last_name":"Jessell"}],"_id":"7698"},{"status":"public","intvolume":"        28","publication":"Current Opinion in Neurobiology","publication_status":"published","citation":{"ama":"Sweeney LB, Kelley DB. Harnessing vocal patterns for social communication. <i>Current Opinion in Neurobiology</i>. 2014;28(10):34-41. doi:<a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">10.1016/j.conb.2014.06.006</a>","mla":"Sweeney, Lora B., and Darcy B. Kelley. “Harnessing Vocal Patterns for Social Communication.” <i>Current Opinion in Neurobiology</i>, vol. 28, no. 10, Elsevier, 2014, pp. 34–41, doi:<a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">10.1016/j.conb.2014.06.006</a>.","ieee":"L. B. Sweeney and D. B. Kelley, “Harnessing vocal patterns for social communication,” <i>Current Opinion in Neurobiology</i>, vol. 28, no. 10. Elsevier, pp. 34–41, 2014.","apa":"Sweeney, L. B., &#38; Kelley, D. B. (2014). Harnessing vocal patterns for social communication. <i>Current Opinion in Neurobiology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">https://doi.org/10.1016/j.conb.2014.06.006</a>","ista":"Sweeney LB, Kelley DB. 2014. Harnessing vocal patterns for social communication. Current Opinion in Neurobiology. 28(10), 34–41.","chicago":"Sweeney, Lora B., and Darcy B Kelley. “Harnessing Vocal Patterns for Social Communication.” <i>Current Opinion in Neurobiology</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.conb.2014.06.006\">https://doi.org/10.1016/j.conb.2014.06.006</a>.","short":"L.B. Sweeney, D.B. Kelley, Current Opinion in Neurobiology 28 (2014) 34–41."},"page":"34-41","publisher":"Elsevier","language":[{"iso":"eng"}],"date_updated":"2024-01-31T10:14:08Z","extern":"1","date_published":"2014-10-01T00:00:00Z","_id":"7699","author":[{"last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"},{"last_name":"Kelley","first_name":"Darcy B","full_name":"Kelley, Darcy B"}],"year":"2014","publication_identifier":{"issn":["0959-4388"]},"type":"journal_article","article_type":"original","month":"10","quality_controlled":"1","title":"Harnessing vocal patterns for social communication","volume":28,"issue":"10","date_created":"2020-04-30T10:35:39Z","oa_version":"None","doi":"10.1016/j.conb.2014.06.006","article_processing_charge":"No","day":"01","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"publication_identifier":{"issn":["0896-6273"]},"month":"05","type":"journal_article","article_type":"original","abstract":[{"text":"Neural circuit assembly requires selection of specific cell fates, axonal trajectories, and synaptic targets. By analyzing the function of a secreted semaphorin, Sema-2b, in Drosophila olfactory receptor neuron (ORN) development, we identified multiple molecular and cellular mechanisms that link these events. Notch signaling limits Sema-2b expression to ventromedial ORN classes, within which Sema-2b cell-autonomously sensitizes ORN axons to external semaphorins. Central-brain-derived Sema-2a and Sema-2b attract Sema-2b-expressing axons to the ventromedial trajectory. In addition, Sema-2b/PlexB-mediated axon-axon interactions consolidate this trajectory choice and promote ventromedial axon-bundle formation. Selecting the correct developmental trajectory is ultimately essential for proper target choice. These findings demonstrate that Sema-2b couples ORN axon guidance to postsynaptic target neuron dendrite patterning well before the final target selection phase, and exemplify how a single guidance molecule can drive consecutive stages of neural circuit assembly with the help of sophisticated spatial and temporal regulation.","lang":"eng"}],"quality_controlled":"1","title":"Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting","volume":78,"doi":"10.1016/j.neuron.2013.03.022","issue":"4","date_created":"2020-04-30T13:19:59Z","oa_version":"None","article_processing_charge":"No","day":"22","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","intvolume":"        78","publication":"Neuron","publication_status":"published","page":"673-686","citation":{"mla":"Joo, William J., et al. “Linking Cell Fate, Trajectory Choice, and Target Selection: Genetic Analysis of Sema-2b in Olfactory Axon Targeting.” <i>Neuron</i>, vol. 78, no. 4, Elsevier, 2013, pp. 673–86, doi:<a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">10.1016/j.neuron.2013.03.022</a>.","ama":"Joo WJ, Sweeney LB, Liang L, Luo L. Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting. <i>Neuron</i>. 2013;78(4):673-686. doi:<a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">10.1016/j.neuron.2013.03.022</a>","apa":"Joo, W. J., Sweeney, L. B., Liang, L., &#38; Luo, L. (2013). Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">https://doi.org/10.1016/j.neuron.2013.03.022</a>","ieee":"W. J. Joo, L. B. Sweeney, L. Liang, and L. Luo, “Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting,” <i>Neuron</i>, vol. 78, no. 4. Elsevier, pp. 673–686, 2013.","short":"W.J. Joo, L.B. Sweeney, L. Liang, L. Luo, Neuron 78 (2013) 673–686.","ista":"Joo WJ, Sweeney LB, Liang L, Luo L. 2013. Linking cell fate, trajectory choice, and target selection: Genetic analysis of sema-2b in olfactory axon targeting. Neuron. 78(4), 673–686.","chicago":"Joo, William J., Lora B. Sweeney, Liang Liang, and Liqun Luo. “Linking Cell Fate, Trajectory Choice, and Target Selection: Genetic Analysis of Sema-2b in Olfactory Axon Targeting.” <i>Neuron</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.neuron.2013.03.022\">https://doi.org/10.1016/j.neuron.2013.03.022</a>."},"publisher":"Elsevier","language":[{"iso":"eng"}],"date_updated":"2024-01-31T10:15:25Z","extern":"1","date_published":"2013-05-22T00:00:00Z","_id":"7785","author":[{"first_name":"William J.","last_name":"Joo","full_name":"Joo, William J."},{"last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601"},{"full_name":"Liang, Liang","last_name":"Liang","first_name":"Liang"},{"first_name":"Liqun","last_name":"Luo","full_name":"Luo, Liqun"}],"year":"2013"},{"author":[{"orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger"},{"last_name":"Chou","first_name":"Ya-Hui","full_name":"Chou, Ya-Hui"},{"full_name":"Wu, Zhuhao","first_name":"Zhuhao","last_name":"Wu"},{"full_name":"Joo, William","last_name":"Joo","first_name":"William"},{"full_name":"Komiyama, Takaki","last_name":"Komiyama","first_name":"Takaki"},{"first_name":"Christopher J.","last_name":"Potter","full_name":"Potter, Christopher J."},{"last_name":"Kolodkin","first_name":"Alex L.","full_name":"Kolodkin, Alex L."},{"first_name":"K. Christopher","last_name":"Garcia","full_name":"Garcia, K. Christopher"},{"last_name":"Luo","first_name":"Liqun","full_name":"Luo, Liqun"}],"year":"2011","_id":"7701","extern":"1","date_published":"2011-12-08T00:00:00Z","date_updated":"2024-01-31T10:13:39Z","language":[{"iso":"eng"}],"publisher":"Elsevier","citation":{"apa":"Sweeney, L. B., Chou, Y.-H., Wu, Z., Joo, W., Komiyama, T., Potter, C. J., … Luo, L. (2011). Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">https://doi.org/10.1016/j.neuron.2011.09.026</a>","mla":"Sweeney, Lora B., et al. “Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting.” <i>Neuron</i>, vol. 72, no. 5, Elsevier, 2011, pp. 734–47, doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">10.1016/j.neuron.2011.09.026</a>.","ieee":"L. B. Sweeney <i>et al.</i>, “Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting,” <i>Neuron</i>, vol. 72, no. 5. Elsevier, pp. 734–747, 2011.","ama":"Sweeney LB, Chou Y-H, Wu Z, et al. Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. <i>Neuron</i>. 2011;72(5):734-747. doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">10.1016/j.neuron.2011.09.026</a>","chicago":"Sweeney, Lora B., Ya-Hui Chou, Zhuhao Wu, William Joo, Takaki Komiyama, Christopher J. Potter, Alex L. Kolodkin, K. Christopher Garcia, and Liqun Luo. “Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting.” <i>Neuron</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.neuron.2011.09.026\">https://doi.org/10.1016/j.neuron.2011.09.026</a>.","ista":"Sweeney LB, Chou Y-H, Wu Z, Joo W, Komiyama T, Potter CJ, Kolodkin AL, Garcia KC, Luo L. 2011. Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting. Neuron. 72(5), 734–747.","short":"L.B. Sweeney, Y.-H. Chou, Z. Wu, W. Joo, T. Komiyama, C.J. Potter, A.L. Kolodkin, K.C. Garcia, L. Luo, Neuron 72 (2011) 734–747."},"page":"734-747","publication":"Neuron","status":"public","intvolume":"        72","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"08","oa_version":"None","doi":"10.1016/j.neuron.2011.09.026","issue":"5","date_created":"2020-04-30T10:36:12Z","article_processing_charge":"No","title":"Secreted semaphorins from degenerating larval ORN axons direct adult projection neuron dendrite targeting","volume":72,"type":"journal_article","article_type":"original","month":"12","abstract":[{"lang":"eng","text":"During assembly of the Drosophila olfactory circuit, projection neuron (PN) dendrites prepattern the developing antennal lobe before the arrival of axons from their presynaptic partners, the adult olfactory receptor neurons (ORNs). We previously found that levels of transmembrane Semaphorin-1a, which acts as a receptor, instruct PN dendrite targeting along the dorsolateral-ventromedial axis. Here we show that two secreted semaphorins, Sema-2a and Sema-2b, provide spatial cues for PN dendrite targeting. Sema-2a and Sema-2b proteins are distributed in gradients opposing the Sema-1a protein gradient, and Sema-1a binds to Sema-2a-expressing cells. In Sema-2a and Sema-2b double mutants, PN dendrites that normally target dorsolaterally in the antennal lobe mistarget ventromedially, phenocopying cell-autonomous Sema-1a removal from these PNs. Cell ablation, cell-specific knockdown, and rescue experiments indicate that secreted semaphorins from degenerating larval ORN axons direct dendrite targeting. Thus, a degenerating brain structure instructs the wiring of a developing circuit through the repulsive action of secreted semaphorins."}],"quality_controlled":"1","publication_identifier":{"issn":["0896-6273"]}},{"extern":"1","date_published":"2011-04-28T00:00:00Z","date_updated":"2024-01-31T10:14:29Z","author":[{"last_name":"Wu","first_name":"Zhuhao","full_name":"Wu, Zhuhao"},{"full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"},{"full_name":"Ayoob, Joseph C.","first_name":"Joseph C.","last_name":"Ayoob"},{"first_name":"Kayam","last_name":"Chak","full_name":"Chak, Kayam"},{"last_name":"Andreone","first_name":"Benjamin J.","full_name":"Andreone, Benjamin J."},{"first_name":"Tomoko","last_name":"Ohyama","full_name":"Ohyama, Tomoko"},{"first_name":"Rex","last_name":"Kerr","full_name":"Kerr, Rex"},{"full_name":"Luo, Liqun","last_name":"Luo","first_name":"Liqun"},{"full_name":"Zlatic, Marta","last_name":"Zlatic","first_name":"Marta"},{"first_name":"Alex L.","last_name":"Kolodkin","full_name":"Kolodkin, Alex L."}],"year":"2011","_id":"7702","page":"281-298","citation":{"ista":"Wu Z, Sweeney LB, Ayoob JC, Chak K, Andreone BJ, Ohyama T, Kerr R, Luo L, Zlatic M, Kolodkin AL. 2011. A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. Neuron. 70(2), 281–298.","chicago":"Wu, Zhuhao, Lora B. Sweeney, Joseph C. Ayoob, Kayam Chak, Benjamin J. Andreone, Tomoko Ohyama, Rex Kerr, Liqun Luo, Marta Zlatic, and Alex L. Kolodkin. “A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS.” <i>Neuron</i>. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">https://doi.org/10.1016/j.neuron.2011.02.050</a>.","short":"Z. Wu, L.B. Sweeney, J.C. Ayoob, K. Chak, B.J. Andreone, T. Ohyama, R. Kerr, L. Luo, M. Zlatic, A.L. Kolodkin, Neuron 70 (2011) 281–298.","apa":"Wu, Z., Sweeney, L. B., Ayoob, J. C., Chak, K., Andreone, B. J., Ohyama, T., … Kolodkin, A. L. (2011). A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">https://doi.org/10.1016/j.neuron.2011.02.050</a>","ieee":"Z. Wu <i>et al.</i>, “A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS,” <i>Neuron</i>, vol. 70, no. 2. Elsevier, pp. 281–298, 2011.","ama":"Wu Z, Sweeney LB, Ayoob JC, et al. A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. <i>Neuron</i>. 2011;70(2):281-298. doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">10.1016/j.neuron.2011.02.050</a>","mla":"Wu, Zhuhao, et al. “A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS.” <i>Neuron</i>, vol. 70, no. 2, Elsevier, 2011, pp. 281–98, doi:<a href=\"https://doi.org/10.1016/j.neuron.2011.02.050\">10.1016/j.neuron.2011.02.050</a>."},"publication":"Neuron","status":"public","intvolume":"        70","publication_status":"published","language":[{"iso":"eng"}],"publisher":"Elsevier","oa_version":"None","date_created":"2020-04-30T10:36:30Z","doi":"10.1016/j.neuron.2011.02.050","issue":"2","article_processing_charge":"No","title":"A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS","volume":70,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"28","publication_identifier":{"issn":["0896-6273"]},"article_type":"original","type":"journal_article","month":"04","abstract":[{"text":"Longitudinal axon fascicles within the Drosophila embryonic CNS provide connections between body segments and are required for coordinated neural signaling along the anterior-posterior axis. We show here that establishment of select CNS longitudinal tracts and formation of precise mechanosensory afferent innervation to the same CNS region are coordinately regulated by the secreted semaphorins Sema-2a and Sema-2b. Both Sema-2a and Sema-2b utilize the same neuronal receptor, plexin B (PlexB), but serve distinct guidance functions. Localized Sema-2b attraction promotes the initial assembly of a subset of CNS longitudinal projections and subsequent targeting of chordotonal sensory afferent axons to these same longitudinal connectives, whereas broader Sema-2a repulsion serves to prevent aberrant innervation. In the absence of Sema-2b or PlexB, chordotonal afferent connectivity within the CNS is severely disrupted, resulting in specific larval behavioral deficits. These results reveal that distinct semaphorin-mediated guidance functions converge at PlexB and are critical for functional neural circuit assembly.","lang":"eng"}],"quality_controlled":"1"},{"language":[{"iso":"eng"}],"publisher":"Elsevier","page":"679-681","citation":{"ista":"Sweeney LB, Luo L. 2010. ‘Fore brain: A hint of the ancestral cortex. Cell. 142(5), 679–681.","chicago":"Sweeney, Lora B., and Liqun Luo. “‘Fore Brain: A Hint of the Ancestral Cortex.” <i>Cell</i>. Elsevier, 2010. <a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">https://doi.org/10.1016/j.cell.2010.08.024</a>.","short":"L.B. Sweeney, L. Luo, Cell 142 (2010) 679–681.","ieee":"L. B. Sweeney and L. Luo, “‘Fore brain: A hint of the ancestral cortex,” <i>Cell</i>, vol. 142, no. 5. Elsevier, pp. 679–681, 2010.","mla":"Sweeney, Lora B., and Liqun Luo. “‘Fore Brain: A Hint of the Ancestral Cortex.” <i>Cell</i>, vol. 142, no. 5, Elsevier, 2010, pp. 679–81, doi:<a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">10.1016/j.cell.2010.08.024</a>.","apa":"Sweeney, L. B., &#38; Luo, L. (2010). ‘Fore brain: A hint of the ancestral cortex. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">https://doi.org/10.1016/j.cell.2010.08.024</a>","ama":"Sweeney LB, Luo L. ‘Fore brain: A hint of the ancestral cortex. <i>Cell</i>. 2010;142(5):679-681. doi:<a href=\"https://doi.org/10.1016/j.cell.2010.08.024\">10.1016/j.cell.2010.08.024</a>"},"status":"public","publication":"Cell","intvolume":"       142","publication_status":"published","author":[{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger"},{"full_name":"Luo, Liqun","last_name":"Luo","first_name":"Liqun"}],"year":"2010","_id":"7703","extern":"1","date_published":"2010-09-03T00:00:00Z","date_updated":"2024-01-31T10:14:59Z","article_type":"original","type":"journal_article","month":"09","quality_controlled":"1","abstract":[{"lang":"eng","text":"By combining gene expression profiling with image registration, Tomer et al. (2010) find that the mushroom body of the segmented worm Platynereis dumerilii shares many features with the mammalian cerebral cortex. The authors propose that the mushroom body and cortex evolved from the same structure in the common ancestor of vertebrates and invertebrates."}],"publication_identifier":{"issn":["0092-8674"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"03","issue":"5","doi":"10.1016/j.cell.2010.08.024","oa_version":"None","date_created":"2020-04-30T10:36:52Z","article_processing_charge":"No","title":"‘Fore brain: A hint of the ancestral cortex","volume":142},{"abstract":[{"lang":"eng","text":"Gradients of axon guidance molecules instruct the formation of continuous neural maps, such as the retinotopic map in the vertebrate visual system. Here we show that molecular gradients can also instruct the formation of a discrete neural map. In the fly olfactory system, axons of 50 classes of olfactory receptor neurons (ORNs) and dendrites of 50 classes of projection neurons (PNs) form one-to-one connections at discrete units called glomeruli. We provide expression, loss- and gain-of-function data to demonstrate that the levels of transmembrane Semaphorin-1a (Sema-1a), acting cell-autonomously as a receptor or part of a receptor complex, direct the dendritic targeting of PNs along the dorsolateral to ventromedial axis of the antennal lobe. Sema-1a also regulates PN axon targeting in higher olfactory centers. Thus, graded expression of Sema-1a contributes to connection specificity from ORNs to PNs and then to higher brain centers, ensuring proper representation of olfactory information in the brain."}],"quality_controlled":"1","type":"journal_article","month":"01","article_type":"original","publication_identifier":{"issn":["0092-8674"]},"day":"26","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":128,"title":"Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons","article_processing_charge":"No","date_created":"2020-04-30T10:37:08Z","oa_version":"None","doi":"10.1016/j.cell.2006.12.028","issue":"2","publisher":"Elsevier","language":[{"iso":"eng"}],"publication_status":"published","publication":"Cell","intvolume":"       128","status":"public","citation":{"ama":"Komiyama T, Sweeney LB, Schuldiner O, Garcia KC, Luo L. Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons. <i>Cell</i>. 2007;128(2):399-410. doi:<a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">10.1016/j.cell.2006.12.028</a>","apa":"Komiyama, T., Sweeney, L. B., Schuldiner, O., Garcia, K. C., &#38; Luo, L. (2007). Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">https://doi.org/10.1016/j.cell.2006.12.028</a>","ieee":"T. Komiyama, L. B. Sweeney, O. Schuldiner, K. C. Garcia, and L. Luo, “Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons,” <i>Cell</i>, vol. 128, no. 2. Elsevier, pp. 399–410, 2007.","mla":"Komiyama, Takaki, et al. “Graded Expression of Semaphorin-1a Cell-Autonomously Directs Dendritic Targeting of Olfactory Projection Neurons.” <i>Cell</i>, vol. 128, no. 2, Elsevier, 2007, pp. 399–410, doi:<a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">10.1016/j.cell.2006.12.028</a>.","chicago":"Komiyama, Takaki, Lora B. Sweeney, Oren Schuldiner, K. Christopher Garcia, and Liqun Luo. “Graded Expression of Semaphorin-1a Cell-Autonomously Directs Dendritic Targeting of Olfactory Projection Neurons.” <i>Cell</i>. Elsevier, 2007. <a href=\"https://doi.org/10.1016/j.cell.2006.12.028\">https://doi.org/10.1016/j.cell.2006.12.028</a>.","ista":"Komiyama T, Sweeney LB, Schuldiner O, Garcia KC, Luo L. 2007. Graded expression of semaphorin-1a cell-autonomously directs dendritic targeting of olfactory projection neurons. Cell. 128(2), 399–410.","short":"T. Komiyama, L.B. Sweeney, O. Schuldiner, K.C. Garcia, L. Luo, Cell 128 (2007) 399–410."},"page":"399-410","_id":"7704","year":"2007","author":[{"full_name":"Komiyama, Takaki","last_name":"Komiyama","first_name":"Takaki"},{"first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601"},{"first_name":"Oren","last_name":"Schuldiner","full_name":"Schuldiner, Oren"},{"first_name":"K. Christopher","last_name":"Garcia","full_name":"Garcia, K. Christopher"},{"full_name":"Luo, Liqun","last_name":"Luo","first_name":"Liqun"}],"date_updated":"2024-01-31T10:14:48Z","date_published":"2007-01-26T00:00:00Z","extern":"1"},{"publication_identifier":{"issn":["0896-6273"]},"type":"journal_article","article_type":"original","month":"01","quality_controlled":"1","abstract":[{"text":"Axon-axon interactions have been implicated in neural circuit assembly, but the underlying mechanisms are poorly understood. Here, we show that in the Drosophila antennal lobe, early-arriving axons of olfactory receptor neurons (ORNs) from the antenna are required for the proper targeting of late-arriving ORN axons from the maxillary palp (MP). Semaphorin-1a is required for targeting of all MP but only half of the antennal ORN classes examined. Sema-1a acts nonautonomously to control ORN axon-axon interactions, in contrast to its cell-autonomous function in olfactory projection neurons. Phenotypic and genetic interaction analyses implicate PlexinA as the Sema-1a receptor in ORN targeting. Sema-1a on antennal ORN axons is required for correct targeting of MP axons within the antennal lobe, while interactions amongst MP axons facilitate their entry into the antennal lobe. We propose that Sema-1a/PlexinA-mediated repulsion provides a mechanism by which early-arriving ORN axons constrain the target choices of late-arriving axons.","lang":"eng"}],"issue":"2","date_created":"2020-04-30T10:37:24Z","doi":"10.1016/j.neuron.2006.12.022","oa_version":"None","article_processing_charge":"No","title":"Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions","volume":53,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"18","page":"185-200","citation":{"chicago":"Sweeney, Lora B., Africa Couto, Ya-Hui Chou, Daniela Berdnik, Barry J. Dickson, Liqun Luo, and Takaki Komiyama. “Temporal Target Restriction of Olfactory Receptor Neurons by Semaphorin-1a/PlexinA-Mediated Axon-Axon Interactions.” <i>Neuron</i>. Elsevier, 2007. <a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">https://doi.org/10.1016/j.neuron.2006.12.022</a>.","ista":"Sweeney LB, Couto A, Chou Y-H, Berdnik D, Dickson BJ, Luo L, Komiyama T. 2007. Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions. Neuron. 53(2), 185–200.","short":"L.B. Sweeney, A. Couto, Y.-H. Chou, D. Berdnik, B.J. Dickson, L. Luo, T. Komiyama, Neuron 53 (2007) 185–200.","mla":"Sweeney, Lora B., et al. “Temporal Target Restriction of Olfactory Receptor Neurons by Semaphorin-1a/PlexinA-Mediated Axon-Axon Interactions.” <i>Neuron</i>, vol. 53, no. 2, Elsevier, 2007, pp. 185–200, doi:<a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">10.1016/j.neuron.2006.12.022</a>.","ama":"Sweeney LB, Couto A, Chou Y-H, et al. Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions. <i>Neuron</i>. 2007;53(2):185-200. doi:<a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">10.1016/j.neuron.2006.12.022</a>","apa":"Sweeney, L. B., Couto, A., Chou, Y.-H., Berdnik, D., Dickson, B. J., Luo, L., &#38; Komiyama, T. (2007). Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2006.12.022\">https://doi.org/10.1016/j.neuron.2006.12.022</a>","ieee":"L. B. Sweeney <i>et al.</i>, “Temporal target restriction of olfactory receptor neurons by semaphorin-1a/plexinA-mediated axon-axon interactions,” <i>Neuron</i>, vol. 53, no. 2. Elsevier, pp. 185–200, 2007."},"status":"public","intvolume":"        53","publication":"Neuron","publication_status":"published","language":[{"iso":"eng"}],"publisher":"Elsevier","extern":"1","date_published":"2007-01-18T00:00:00Z","date_updated":"2024-01-31T10:14:39Z","author":[{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger"},{"first_name":"Africa","last_name":"Couto","full_name":"Couto, Africa"},{"full_name":"Chou, Ya-Hui","last_name":"Chou","first_name":"Ya-Hui"},{"last_name":"Berdnik","first_name":"Daniela","full_name":"Berdnik, Daniela"},{"first_name":"Barry J.","last_name":"Dickson","full_name":"Dickson, Barry J."},{"first_name":"Liqun","last_name":"Luo","full_name":"Luo, Liqun"},{"full_name":"Komiyama, Takaki","first_name":"Takaki","last_name":"Komiyama"}],"year":"2007","_id":"7705"},{"language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","page":"2011-2015","citation":{"chicago":"Brunet, Anne, Lora B. Sweeney, J Fitzhugh  Sturgill, Katrin Chua, Paul Greer, Yingxi Lin, Hien Tran, et al. “Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase.” <i>Science</i>. American Association for the Advancement of Science, 2004. <a href=\"https://doi.org/10.1126/science.1094637\">https://doi.org/10.1126/science.1094637</a>.","ista":"Brunet A, Sweeney LB, Sturgill JF, Chua K, Greer P, Lin Y, Tran H, Ross S, Mostoslavsky R, Cohen H, Hu L, Chen H-L, Jedrychowski M, Gygi S, Sinclair D, Alt F, Greenberg M. 2004. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 303(5666), 2011–2015.","short":"A. Brunet, L.B. Sweeney, J.F. Sturgill, K. Chua, P. Greer, Y. Lin, H. Tran, S. Ross, R. Mostoslavsky, H. Cohen, L. Hu, H.-L. Chen, M. Jedrychowski, S. Gygi, D. Sinclair, F. Alt, M. Greenberg, Science 303 (2004) 2011–2015.","mla":"Brunet, Anne, et al. “Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase.” <i>Science</i>, vol. 303, no. 5666, American Association for the Advancement of Science, 2004, pp. 2011–15, doi:<a href=\"https://doi.org/10.1126/science.1094637\">10.1126/science.1094637</a>.","ama":"Brunet A, Sweeney LB, Sturgill JF, et al. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. <i>Science</i>. 2004;303(5666):2011-2015. doi:<a href=\"https://doi.org/10.1126/science.1094637\">10.1126/science.1094637</a>","apa":"Brunet, A., Sweeney, L. B., Sturgill, J. F., Chua, K., Greer, P., Lin, Y., … Greenberg, M. (2004). Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1094637\">https://doi.org/10.1126/science.1094637</a>","ieee":"A. Brunet <i>et al.</i>, “Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase,” <i>Science</i>, vol. 303, no. 5666. American Association for the Advancement of Science, pp. 2011–2015, 2004."},"publication_status":"published","publication":"Science","status":"public","intvolume":"       303","year":"2004","author":[{"first_name":"Anne","last_name":"Brunet","full_name":"Brunet, Anne"},{"first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601"},{"full_name":"Sturgill, J Fitzhugh ","first_name":"J Fitzhugh ","last_name":"Sturgill"},{"last_name":"Chua","first_name":"Katrin","full_name":"Chua, Katrin"},{"full_name":"Greer, Paul","last_name":"Greer","first_name":"Paul"},{"last_name":"Lin","first_name":"Yingxi","full_name":"Lin, Yingxi"},{"full_name":"Tran, Hien","first_name":"Hien","last_name":"Tran"},{"full_name":"Ross, Sarah","first_name":"Sarah","last_name":"Ross"},{"full_name":"Mostoslavsky, Raul","last_name":"Mostoslavsky","first_name":"Raul"},{"full_name":"Cohen, Haim","last_name":"Cohen","first_name":"Haim"},{"last_name":"Hu","first_name":"Linda","full_name":"Hu, Linda"},{"last_name":"Chen","first_name":"Hwei-Ling","full_name":"Chen, Hwei-Ling"},{"first_name":"Mark","last_name":"Jedrychowski","full_name":"Jedrychowski, Mark"},{"full_name":"Gygi, Steven","first_name":"Steven","last_name":"Gygi"},{"full_name":"Sinclair, David","first_name":"David","last_name":"Sinclair"},{"first_name":"Frederick","last_name":"Alt","full_name":"Alt, Frederick"},{"last_name":"Greenberg","first_name":"Michael","full_name":"Greenberg, Michael"}],"_id":"7706","date_published":"2004-03-26T00:00:00Z","extern":"1","date_updated":"2024-01-31T10:14:17Z","quality_controlled":"1","abstract":[{"text":"The Sir2 deacetylase modulates organismal life-span in various species. However, the molecular mechanisms by which Sir2 increases longevity are largely unknown. We show that in mammalian cells, the Sir2 homolog SIRT1 appears to control the cellular response to stress by regulating the FOXO family of Forkhead transcription factors, a family of proteins that function as sensors of the insulin signaling pathway and as regulators of organismal longevity. SIRT1 and the FOXO transcription factor FOXO3 formed a complex in cells in response to oxidative stress, and SIRT1 deacetylated FOXO3 in vitro and within cells. SIRT1 had a dual effect on FOXO3 function: SIRT1 increased FOXO3's ability to induce cell cycle arrest and resistance to oxidative stress but inhibited FOXO3's ability to induce cell death. Thus, one way in which members of the Sir2 family of proteins may increase organismal longevity is by tipping FOXO-dependent responses away from apoptosis and toward stress resistance.","lang":"eng"}],"type":"journal_article","article_type":"original","month":"03","publication_identifier":{"issn":["0036-8075","1095-9203"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"26","article_processing_charge":"No","doi":"10.1126/science.1094637","date_created":"2020-04-30T10:37:41Z","oa_version":"None","issue":"5666","volume":303,"title":"Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase"}]