---
OA_place: publisher
OA_type: hybrid
_id: '20029'
abstract:
- lang: eng
  text: Vacuolar acidification is crucial for the homeostasis of intracellular pH
    and the recycling of proteins and nutrients in cells, thereby playing important
    roles in various physiological processes related to vacuolar function. The key
    factors regulating vacuolar acidification and underlying mechanisms remain unclear.
    Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification
    of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency.
    The pldζ2 mutant massively accumulates autophagic structures while exhibiting
    premature leaf senescence under nutrient starvation. Impaired autophagic flux,
    lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates
    autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast
    and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2
    directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote
    vacuolar acidification and autophagy under phosphorus starvation. These findings
    reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide
    clues in elucidating the regulatory mechanism of vacuolar acidification.
acknowledgement: The study was supported by National Natural Science Foundation of
  China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were
  run on the Siyuan-1 cluster supported by the Center for High-Performance Computing
  at Shanghai Jiao Tong University.
article_number: '116024'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Bin
  full_name: Guan, Bin
  id: 56aad729-cca2-11ed-a45a-9b4138991a48
  last_name: Guan
- first_name: Ke Xuan
  full_name: Xie, Ke Xuan
  last_name: Xie
- first_name: Xin Qiao
  full_name: Du, Xin Qiao
  last_name: Du
- first_name: Yu Xuan
  full_name: Bai, Yu Xuan
  last_name: Bai
- first_name: Peng Chao
  full_name: Hao, Peng Chao
  last_name: Hao
- first_name: Wen Hui
  full_name: Lin, Wen Hui
  last_name: Lin
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Hong Wei
  full_name: Xue, Hong Wei
  last_name: Xue
citation:
  ama: Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar
    acidification and autophagy under phosphorus starvation by interacting with VATD.
    <i>Cell Reports</i>. 2025;44(7). doi:<a href="https://doi.org/10.1016/j.celrep.2025.116024">10.1016/j.celrep.2025.116024</a>
  apa: Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue,
    H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification
    and autophagy under phosphorus starvation by interacting with VATD. <i>Cell Reports</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.celrep.2025.116024">https://doi.org/10.1016/j.celrep.2025.116024</a>
  chicago: Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui
    Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates
    Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting
    with VATD.” <i>Cell Reports</i>. Elsevier, 2025. <a href="https://doi.org/10.1016/j.celrep.2025.116024">https://doi.org/10.1016/j.celrep.2025.116024</a>.
  ieee: B. Guan <i>et al.</i>, “Arabidopsis phospholipase Dζ2 facilitates vacuolar
    acidification and autophagy under phosphorus starvation by interacting with VATD,”
    <i>Cell Reports</i>, vol. 44, no. 7. Elsevier, 2025.
  ista: Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis
    phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus
    starvation by interacting with VATD. Cell Reports. 44(7), 116024.
  mla: Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification
    and Autophagy under Phosphorus Starvation by Interacting with VATD.” <i>Cell Reports</i>,
    vol. 44, no. 7, 116024, Elsevier, 2025, doi:<a href="https://doi.org/10.1016/j.celrep.2025.116024">10.1016/j.celrep.2025.116024</a>.
  short: B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W.
    Xue, Cell Reports 44 (2025).
date_created: 2025-07-20T22:02:01Z
date_published: 2025-07-22T00:00:00Z
date_updated: 2025-09-30T14:05:28Z
day: '22'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.celrep.2025.116024
external_id:
  isi:
  - '001533244800001'
  pmid:
  - '40668679'
file:
- access_level: open_access
  checksum: ee03deee47a084b0295251dc49470ad4
  content_type: application/pdf
  creator: dernst
  date_created: 2025-07-22T08:52:17Z
  date_updated: 2025-07-22T08:52:17Z
  file_id: '20067'
  file_name: 2025_CellReports_Guan.pdf
  file_size: 37708120
  relation: main_file
  success: 1
file_date_updated: 2025-07-22T08:52:17Z
has_accepted_license: '1'
intvolume: '        44'
isi: 1
issue: '7'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy
  under phosphorus starvation by interacting with VATD
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 44
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '20116'
abstract:
- lang: eng
  text: Auxin regulates various aspects of plant growth and development by modulating
    the transcription of target genes through the degradation of auxin/indole-3-acetic
    acid (Aux/IAA) repressors via the 26S proteasome. Proteasome regulator 1 (PTRE1),
    a positive regulator of proteasome activity, has been implicated in auxin-mediated
    proteasome suppression; however, the mechanism by which auxin modulates PTRE1
    function remains unclear. Here, we demonstrate that auxin promotes the interaction
    between germin-like protein 1 (GLP1) and PTRE1, facilitating PTRE1 retention at
    the plasma membrane. The relocation of PTRE1 results in reduced nuclear 26S proteasome
    activity, and thus the attenuated Aux/IAA degradation and altered Aux/IAA homeostasis,
    ultimately resulting in suppressed auxin-mediated transcriptional regulation.
    Our findings uncover a previously uncharacterized regulatory axis in auxin signaling
    that controls Aux/IAA protein stability, functioning alongside the TIR1- and TRANSMEMBRANE
    KINASE 1 (TMK1)-mediated pathways, and highlight the coordination of auxin signaling
    from the cell surface to the nucleus via auxin-induced PTRE1 relocation, which
    fine-tunes Aux/IAA protein homeostasis and auxin responses.
acknowledgement: The study was supported by the National Natural Science Foundation
  of China (NSFC; 32230011, 91954206, and 31721001). We thank Dr. Deli Lin (Shanghai
  Jiao Tong University) for kind help with the laser confocal microscope observation
  and the Arabidopsis Biological Resource Center (ABRC) for providing T-DNA insertional
  mutants.
article_number: '116056'
article_processing_charge: Yes
article_type: original
author:
- first_name: Faqing
  full_name: Xu, Faqing
  last_name: Xu
- first_name: Yongqiang
  full_name: Yu, Yongqiang
  last_name: Yu
- first_name: Bin
  full_name: Guan, Bin
  id: 56aad729-cca2-11ed-a45a-9b4138991a48
  last_name: Guan
- first_name: Tongda
  full_name: Xu, Tongda
  last_name: Xu
- first_name: Zhihong
  full_name: Xu, Zhihong
  last_name: Xu
- first_name: Hongwei
  full_name: Xue, Hongwei
  last_name: Xue
citation:
  ama: Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. Germin-like protein 1 interacts with
    proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA homeostasis.
    <i>Cell Reports</i>. 2025;44(8). doi:<a href="https://doi.org/10.1016/j.celrep.2025.116056">10.1016/j.celrep.2025.116056</a>
  apa: Xu, F., Yu, Y., Guan, B., Xu, T., Xu, Z., &#38; Xue, H. (2025). Germin-like
    protein 1 interacts with proteasome regulator 1 to regulate auxin signaling by
    controlling Aux/IAA homeostasis. <i>Cell Reports</i>. Elsevier. <a href="https://doi.org/10.1016/j.celrep.2025.116056">https://doi.org/10.1016/j.celrep.2025.116056</a>
  chicago: Xu, Faqing, Yongqiang Yu, Bin Guan, Tongda Xu, Zhihong Xu, and Hongwei
    Xue. “Germin-like Protein 1 Interacts with Proteasome Regulator 1 to Regulate
    Auxin Signaling by Controlling Aux/IAA Homeostasis.” <i>Cell Reports</i>. Elsevier,
    2025. <a href="https://doi.org/10.1016/j.celrep.2025.116056">https://doi.org/10.1016/j.celrep.2025.116056</a>.
  ieee: F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, and H. Xue, “Germin-like protein 1 interacts
    with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA
    homeostasis,” <i>Cell Reports</i>, vol. 44, no. 8. Elsevier, 2025.
  ista: Xu F, Yu Y, Guan B, Xu T, Xu Z, Xue H. 2025. Germin-like protein 1 interacts
    with proteasome regulator 1 to regulate auxin signaling by controlling Aux/IAA
    homeostasis. Cell Reports. 44(8), 116056.
  mla: Xu, Faqing, et al. “Germin-like Protein 1 Interacts with Proteasome Regulator
    1 to Regulate Auxin Signaling by Controlling Aux/IAA Homeostasis.” <i>Cell Reports</i>,
    vol. 44, no. 8, 116056, Elsevier, 2025, doi:<a href="https://doi.org/10.1016/j.celrep.2025.116056">10.1016/j.celrep.2025.116056</a>.
  short: F. Xu, Y. Yu, B. Guan, T. Xu, Z. Xu, H. Xue, Cell Reports 44 (2025).
date_created: 2025-08-04T13:39:11Z
date_published: 2025-07-24T00:00:00Z
date_updated: 2025-09-30T14:13:45Z
day: '24'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.celrep.2025.116056
external_id:
  isi:
  - '001542038500001'
  pmid:
  - '40714631'
file:
- access_level: open_access
  checksum: 3c43e040a4a7a65ec67ae1d2bb81261a
  content_type: application/pdf
  creator: dernst
  date_created: 2025-08-05T06:15:09Z
  date_updated: 2025-08-05T06:15:09Z
  file_id: '20120'
  file_name: 2025_CellReports_Xu.pdf
  file_size: 24178018
  relation: main_file
  success: 1
file_date_updated: 2025-08-05T06:15:09Z
has_accepted_license: '1'
intvolume: '        44'
isi: 1
issue: '8'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Germin-like protein 1 interacts with proteasome regulator 1 to regulate auxin
  signaling by controlling Aux/IAA homeostasis
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 44
year: '2025'
...