[{"ddc":["580"],"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"date_created":"2026-06-13T16:57:07Z","type":"preprint","publication":"bioRxiv","abstract":[{"text":"Adaptive plant development is orchestrated, among others, by directional, intercellular transport of the phytohormone auxin. Self-organizing development, such as flexible vasculature formation, depends on so-called auxin canalization, manifested by the gradual formation of auxin transport channels through feedback between auxin signalling and transport. Herein, we identify MAKR6 as an important, novel component in this feedback. MAKR6 expression accumulates strongly in vascular cells and is tightly regulated by auxin via the Aux/IAA-ARF-WRKY23 transcriptional network. MAKR6 is required for auxin canalization-dependent processes, including leaf venation, vasculature regeneration, and de novo auxin channel formation from local auxin sources. Mechanistically, MAKR6 interacts with the PIN1 auxin transporter, modulating its trafficking and polarization. MAKR6 also associates with and integrates two key receptor-like kinase complexes involved in canalization, TMK1/4 and the CAMEL-CANAR. Together, our study establishes MAKR6 as a multifaceted regulator that couples transcriptional auxin signalling to PIN1 repolarization and coordinates multiple RLK-mediated signalling pathways during canalization. This provides mechanistic insights into auxin canalization and exemplifies a framework for exploring similar regulatory nodes in other developmental contexts.","lang":"eng"}],"publication_status":"submitted","citation":{"ama":"Ge Z, Koczka L, Mazur E, et al. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.07.680881\">10.1101/2025.10.07.680881</a>","chicago":"Ge, Zengxiang, Lilla Koczka, Ewa Mazur, Gergely Molnar, Dmitrii Vladimirtsev, Nada Kassem, Sara Ait Ikene, Lukas Fiedler, and Jiří Friml. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.07.680881\">https://doi.org/10.1101/2025.10.07.680881</a>.","apa":"Ge, Z., Koczka, L., Mazur, E., Molnar, G., Vladimirtsev, D., Kassem, N., … Friml, J. (n.d.). MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.07.680881\">https://doi.org/10.1101/2025.10.07.680881</a>","ieee":"Z. Ge <i>et al.</i>, “MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis,” <i>bioRxiv</i>. .","short":"Z. Ge, L. Koczka, E. Mazur, G. Molnar, D. Vladimirtsev, N. Kassem, S. Ait Ikene, L. Fiedler, J. Friml, BioRxiv (n.d.).","mla":"Ge, Zengxiang, et al. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.07.680881\">10.1101/2025.10.07.680881</a>.","ista":"Ge Z, Koczka L, Mazur E, Molnar G, Vladimirtsev D, Kassem N, Ait Ikene S, Fiedler L, Friml J. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.07.680881\">10.1101/2025.10.07.680881</a>."},"date_updated":"2026-06-19T07:14:01Z","_id":"21994","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis","author":[{"full_name":"Ge, Zengxiang","last_name":"Ge","orcid":"0000-0001-9381-3577","first_name":"Zengxiang","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8"},{"full_name":"Koczka, Lilla","last_name":"Koczka","first_name":"Lilla"},{"full_name":"Mazur, Ewa","last_name":"Mazur","first_name":"Ewa"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely","last_name":"Molnar","full_name":"Molnar, Gergely"},{"first_name":"Dmitrii","last_name":"Vladimirtsev","full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"full_name":"Kassem, Nada","last_name":"Kassem","first_name":"Nada"},{"last_name":"Ait Ikene","full_name":"Ait Ikene, Sara","first_name":"Sara","id":"6a0bb896-6bad-11f1-9bef-906e9eb76034"},{"id":"7c417475-8972-11ed-ae7b-8b674ca26986","full_name":"Fiedler, Lukas","last_name":"Fiedler","first_name":"Lukas"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"acknowledgement":"We would like to thank Dr. Yvon Jaillais (ENS, Lyon) for sharing MAKR2 materials. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The research in the Friml group leading to these results was funded by the European Research Council (ERC): 101142681 CYNIPS; and the Austrian Science Fund (FWF): I 6123-B and P 37051-B. Ewa Mazur was supported by the National Science Centre (NCN), Poland, under the OPUS call in the WEAVE programme: 2021/43/I/NZ1/01835.","corr_author":"1","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"date_published":"2026-05-30T00:00:00Z","oa":1,"OA_type":"green","oa_version":"Preprint","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"status":"public","language":[{"iso":"eng"}],"article_processing_charge":"No","scopus_import":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.10.07.680881"}],"day":"30","has_accepted_license":"1","OA_place":"repository","doi":"10.1101/2025.10.07.680881","month":"05"},{"oa_version":"Published Version","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"status":"public","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"}],"date_published":"2026-01-14T00:00:00Z","alternative_title":["ISTA Master’s Thesis"],"page":"22","author":[{"full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev","first_name":"Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"}],"corr_author":"1","month":"01","file_date_updated":"2026-01-28T12:38:19Z","doi":"10.15479/AT-ISTA-20964","OA_place":"publisher","has_accepted_license":"1","day":"14","language":[{"iso":"eng"}],"article_processing_charge":"No","publication_status":"published","degree_awarded":"MS","type":"dissertation","date_created":"2026-01-09T09:22:48Z","ddc":["570"],"file":[{"file_name":"2026_Vladimirtsev_Dmitrii_Thesis.pdf","checksum":"812857b2fbe3f6113bef22fd04bccd3e","file_id":"21033","access_level":"closed","relation":"main_file","file_size":2867531,"date_created":"2026-01-21T14:12:13Z","date_updated":"2026-01-21T14:12:13Z","embargo_to":"open_access","content_type":"application/pdf","embargo":"2027-01-01","creator":"dvladimi"},{"relation":"source_file","file_size":25023066,"access_level":"closed","file_id":"21034","file_name":"Source Files.zip","checksum":"2b969f97f8d7461bea3d255f48c2219c","creator":"dvladimi","content_type":"application/x-zip-compressed","date_updated":"2026-01-28T12:38:19Z","date_created":"2026-01-21T14:41:58Z"}],"related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"20982"}]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publisher":"Institute of Science and Technology Austria","title":"Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels","supervisor":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"year":"2026","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"_id":"20964","date_updated":"2026-04-07T11:41:44Z","publication_identifier":{"issn":["2791-4585"]},"citation":{"ama":"Vladimirtsev D. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20964\">10.15479/AT-ISTA-20964</a>","chicago":"Vladimirtsev, Dmitrii. “Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels.” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-20964\">https://doi.org/10.15479/AT-ISTA-20964</a>.","apa":"Vladimirtsev, D. (2026). <i>Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20964\">https://doi.org/10.15479/AT-ISTA-20964</a>","ieee":"D. Vladimirtsev, “Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels,” Institute of Science and Technology Austria, 2026.","short":"D. Vladimirtsev, Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels, Institute of Science and Technology Austria, 2026.","mla":"Vladimirtsev, Dmitrii. <i>Armadillo Repeat Only Proteins Are Master Regulators of Plant Cyclic-Nucleotide Gated Channels</i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20964\">10.15479/AT-ISTA-20964</a>.","ista":"Vladimirtsev D. 2026. Armadillo repeat only proteins are master regulators of plant cyclic-nucleotide gated channels. Institute of Science and Technology Austria."}},{"day":"16","language":[{"iso":"eng"}],"article_processing_charge":"No","scopus_import":"1","issue":"6795","OA_place":"repository","has_accepted_license":"1","doi":"10.1126/science.adu8197","month":"04","file_date_updated":"2026-05-07T05:54:43Z","page":"296-300","author":[{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","full_name":"Kulich, Ivan","first_name":"Ivan"},{"full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev","first_name":"Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"first_name":"Marek","full_name":"Randuch, Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Shiqiang","full_name":"Gao, Shiqiang","last_name":"Gao"},{"first_name":"Matteo","last_name":"Citterico","full_name":"Citterico, Matteo"},{"full_name":"Konrad, Kai R.","last_name":"Konrad","first_name":"Kai R."},{"last_name":"Nagel","full_name":"Nagel, Georg","first_name":"Georg"},{"full_name":"Wrzaczek, Michael","last_name":"Wrzaczek","first_name":"Michael"},{"first_name":"Léa","full_name":"Cascaro, Léa","last_name":"Cascaro"},{"last_name":"Vinet","full_name":"Vinet, Pauline","first_name":"Pauline"},{"first_name":"Pauline","full_name":"Durand, Pauline","last_name":"Durand"},{"first_name":"Atef","last_name":"Asnacios","full_name":"Asnacios, Atef"},{"last_name":"Verma","full_name":"Verma, Lokesh","first_name":"Lokesh"},{"first_name":"Malcolm J.","last_name":"Bennett","full_name":"Bennett, Malcolm J."},{"first_name":"Bipin K.","last_name":"Pandey","full_name":"Pandey, Bipin K."},{"last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"corr_author":"1","acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","intvolume":"       392","OA_type":"green","oa":1,"external_id":{"pmid":["41990180"]},"project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"date_published":"2026-04-16T00:00:00Z","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"oa_version":"Accepted Version","status":"public","quality_controlled":"1","publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]},"citation":{"apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>.","mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>.","ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300.","ieee":"I. Kulich <i>et al.</i>, “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” <i>Science</i>, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300.","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. 2026;392(6795):296-300. doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>"},"_id":"21763","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"pmid":1,"date_updated":"2026-05-07T06:20:07Z","year":"2026","publisher":"AAAS","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","ddc":["580"],"file":[{"creator":"dernst","content_type":"application/pdf","date_updated":"2026-05-07T05:54:43Z","date_created":"2026-05-07T05:54:43Z","relation":"main_file","success":1,"file_size":6150733,"access_level":"open_access","file_id":"21832","file_name":"2026_Science_Kulich_accepted.pdf","checksum":"eb5b29247832ecdc53c8146da0509bbe"}],"type":"journal_article","date_created":"2026-04-26T22:01:47Z","volume":392,"publication":"Science","publication_status":"published","article_type":"original","abstract":[{"text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil.","lang":"eng"}]},{"ddc":["580"],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"type":"journal_article","date_created":"2026-07-13T10:44:55Z","publication":"Journal of Integrative Plant Biology","article_type":"original","publication_status":"epub_ahead","article_number":"jipb.70309","abstract":[{"text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage.","lang":"eng"}],"publication_identifier":{"issn":["1672-9072"],"eissn":["1744-7909"]},"citation":{"chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>.","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","ieee":"A. Smoljan <i>et al.</i>, “Auxin response and PIN‐mediated transport in chlorophyte algae,” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026.","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>, jipb. 70309, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>.","ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309.","ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. 2026. doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>"},"_id":"22301","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"date_updated":"2026-07-13T14:26:31Z","pmid":1,"year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Wiley","title":"Auxin response and PIN‐mediated transport in chlorophyte algae","author":[{"id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana","last_name":"Smoljan","full_name":"Smoljan, Adrijana"},{"first_name":"Sarah","last_name":"Koutnik‐Abele","full_name":"Koutnik‐Abele, Sarah"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii","last_name":"Vladimirtsev","full_name":"Vladimirtsev, Dmitrii"},{"last_name":"Klíma","full_name":"Klíma, Petr","first_name":"Petr"},{"full_name":"Bírošíková, Anita","last_name":"Bírošíková","first_name":"Anita"},{"orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou","last_name":"Zhang","first_name":"Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"37e65def-d415-11eb-ae59-a7b67be103db","full_name":"Schuster, Maximilian","last_name":"Schuster","first_name":"Maximilian"},{"first_name":"Katarina","full_name":"Kurtović, Katarina","last_name":"Kurtović"},{"first_name":"Ulrich Z.","last_name":"Hammes","full_name":"Hammes, Ulrich Z."},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"corr_author":"1","supplementarymaterial":"yes","acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","OA_type":"hybrid","oa":1,"project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"external_id":{"pmid":["42271607"]},"date_published":"2026-06-10T00:00:00Z","oa_version":"Published Version","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"quality_controlled":"1","status":"public","main_file_link":[{"url":"https://doi.org/10.1111/jipb.70309","open_access":"1"}],"day":"10","article_processing_charge":"Yes (via OA deal)","language":[{"iso":"eng"}],"scopus_import":"1","researchdata_availability":"no","OA_place":"publisher","has_accepted_license":"1","doi":"10.1111/jipb.70309","das_tickbox":"0","month":"06","PlanS_conform":"1"},{"status":"public","oa_version":"Preprint","department":[{"_id":"JiFr"}],"abstract":[{"lang":"eng","text":"Plant cells respond to a wide range of stimuli through intracellular calcium (Ca2+) signaling. Cyclic nucleotide-gated channels (CNGCs) are a major class of plant Ca2+ channels, with 20 homologs in Arabidopsis. These tetrameric plasma membrane proteins act downstream of diverse signals, such as phytohormones, extracellular damage, cell wall integrity or temperature. Here, we identify a class of plant-specific proteins, Armadillo Repeat Only (ARO), as essential regulators of possibly all plant CNGCs. Abrogation of functional sporophytic AROs results in a phenotypic pattern strongly reminiscent of CNGC dysfunction, including defects in root gravitropism, root hair growth and morphology, stomatal movement, and responses to extracellular ATP and the phytohormone auxin. aro2/3/4 mutants are fully resistant to the toxic effects caused by overexpression of CNGCs. AROs colocalize and physically interact with multiple CNGCs and modulate CNGC-dependent currents in Xenopus oocytes. Structural modeling and site-directed mutagenesis reveal AROs tetramer formation surrounding the CNGC channel, interacting via its IQ domain. Taken together, plant CNGC channels don’t act alone, but in a larger complex - channelosome, first of a kind in plants."}],"publication_status":"draft","date_published":"2025-05-16T00:00:00Z","publication":"bioRxiv","oa":1,"date_created":"2026-01-13T14:07:58Z","type":"preprint","corr_author":"1","acknowledgement":"This project was supported by the Czech Science Foundation grant Nr. 25-16449S and by European\r\nUnion, Horizon Europe, project MOLIPEC, ID 101087030. Computational resources used for structural\r\nmodeling were provided by the e-INFRA CZ project (ID:90254), supported by the Ministry of Education,\r\nYouth and Sports of the Czech Republic. Part of the work was carried out with the support of a Growth\r\nFacility (BC Core Facilities; IPMB BC CAS). X. laevis oocytes were kindly provided by C. Korbmacher on\r\na regular basis (FAU Erlangen-Nürnberg). MF received support from the European Research Council\r\n(Grant 480 No. 101125499). We acknowledge the core facility LMH, the BC CAS supported by the MEYS\r\nCR (LM 2023050 Czech-BioImaging). DO received support from the Czech Science Foundation grant Nr.\r\n24-12107S\r\n","author":[{"full_name":"Kulich, Ivan","last_name":"Kulich","first_name":"Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"first_name":"Denisa","full_name":"Oulehlová, Denisa","last_name":"Oulehlová"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev"},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia","last_name":"Zou","full_name":"Zou, Minxia"},{"last_name":"Lileikyte","full_name":"Lileikyte, Edita","first_name":"Edita"},{"first_name":"Alexey","last_name":"Bondar","full_name":"Bondar, Alexey"},{"first_name":"Katarína","last_name":"Kulichová","full_name":"Kulichová, Katarína"},{"full_name":"Janda, Martin","last_name":"Janda","first_name":"Martin"},{"first_name":"Oksana","last_name":"Iakovenko","full_name":"Iakovenko, Oksana"},{"first_name":"Michaela","last_name":"Neubergerová","full_name":"Neubergerová, Michaela"},{"full_name":"Studtrucker, Tanja","last_name":"Studtrucker","first_name":"Tanja"},{"first_name":"Roman","full_name":"Pleskot, Roman","last_name":"Pleskot"},{"first_name":"Petra","full_name":"Dietrich, Petra","last_name":"Dietrich"},{"id":"43905548-F248-11E8-B48F-1D18A9856A87","first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych","orcid":"0000-0002-9767-8699"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"related_material":{"record":[{"status":"public","id":"20964","relation":"dissertation_contains"}]},"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"month":"05","title":"Armadillo repeat only proteins are required for the function of plant CNGC channels","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","year":"2025","doi":"10.1101/2025.01.06.631460","date_updated":"2026-04-07T11:41:43Z","OA_place":"repository","_id":"20982","citation":{"ista":"Kulich I, Oulehlová D, Vladimirtsev D, Zou M, Lileikyte E, Bondar A, Kulichová K, Janda M, Iakovenko O, Neubergerová M, Studtrucker T, Pleskot R, Dietrich P, Fendrych M, Friml J. Armadillo repeat only proteins are required for the function of plant CNGC channels. bioRxiv, <a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>.","mla":"Kulich, Ivan, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>.","ieee":"I. Kulich <i>et al.</i>, “Armadillo repeat only proteins are required for the function of plant CNGC channels,” <i>bioRxiv</i>. .","short":"I. Kulich, D. Oulehlová, D. Vladimirtsev, M. Zou, E. Lileikyte, A. Bondar, K. Kulichová, M. Janda, O. Iakovenko, M. Neubergerová, T. Studtrucker, R. Pleskot, P. Dietrich, M. Fendrych, J. Friml, BioRxiv (n.d.).","apa":"Kulich, I., Oulehlová, D., Vladimirtsev, D., Zou, M., Lileikyte, E., Bondar, A., … Friml, J. (n.d.). Armadillo repeat only proteins are required for the function of plant CNGC channels. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.01.06.631460\">https://doi.org/10.1101/2025.01.06.631460</a>","chicago":"Kulich, Ivan, Denisa Oulehlová, Dmitrii Vladimirtsev, Minxia Zou, Edita Lileikyte, Alexey Bondar, Katarína Kulichová, et al. “Armadillo Repeat Only Proteins Are Required for the Function of Plant CNGC Channels.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.01.06.631460\">https://doi.org/10.1101/2025.01.06.631460</a>.","ama":"Kulich I, Oulehlová D, Vladimirtsev D, et al. Armadillo repeat only proteins are required for the function of plant CNGC channels. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.01.06.631460\">10.1101/2025.01.06.631460</a>"},"article_processing_charge":"No","language":[{"iso":"eng"}],"day":"16","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.01.06.631460"}]},{"article_type":"original","publication_status":"published","abstract":[{"text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a prerequisite for (1) self-organizing processes, including vascular tissue formation, and (2) directional growth responses such as gravitropism. Here, we identify a mechanism by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1) receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably for other self-organizing developmental phenomena.","lang":"eng"}],"volume":188,"publication":"Cell","type":"journal_article","date_created":"2025-11-19T09:44:31Z","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"related_material":{"record":[{"id":"19399","relation":"earlier_version","status":"public"}]},"file":[{"creator":"dernst","date_created":"2025-11-24T10:55:18Z","date_updated":"2025-11-24T10:55:18Z","content_type":"application/pdf","access_level":"open_access","file_size":17825465,"success":1,"relation":"main_file","checksum":"8ac396a0806ad7f2e4e7a0c1eed712ce","file_name":"2025_Cell_Rodriguez.pdf","file_id":"20679"}],"ddc":["580"],"ec_funded":1,"title":"ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism","publisher":"Elsevier","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","_id":"20656","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"pmid":1,"date_updated":"2026-07-06T12:51:13Z","publication_identifier":{"issn":["0092-8674"]},"citation":{"ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. <i>Cell</i>. 2025;188(22):6138-6150.e17. doi:<a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">10.1016/j.cell.2025.08.026</a>","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">https://doi.org/10.1016/j.cell.2025.08.026</a>.","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (2025). ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">https://doi.org/10.1016/j.cell.2025.08.026</a>","ieee":"L. Rodriguez Solovey <i>et al.</i>, “ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism,” <i>Cell</i>, vol. 188, no. 22. Elsevier, p. 6138–6150.e17, 2025.","short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, I. Kulich, Y. Jaillais, J. Friml, Cell 188 (2025) 6138–6150.e17.","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Kulich I, Jaillais Y, Friml J. 2025. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 188(22), 6138–6150.e17.","mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>Cell</i>, vol. 188, no. 22, Elsevier, 2025, p. 6138–6150.e17, doi:<a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">10.1016/j.cell.2025.08.026</a>."},"status":"public","quality_controlled":"1","department":[{"_id":"JiFr"},{"_id":"XiFe"}],"oa_version":"Published Version","OA_type":"hybrid","oa":1,"date_published":"2025-10-30T00:00:00Z","external_id":{"isi":["001616077900005"],"pmid":["41043433"]},"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123"},{"grant_number":"ALTF 985-2016","_id":"26060676-B435-11E9-9278-68D0E5697425","name":"Cell surface receptor complexes for auxin signaling in plants"},{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}],"intvolume":"       188","isi":1,"page":"6138-6150.e17","acknowledgement":"We gratefully acknowledge Tongda Xu for experimental, material, and conceptual support. We thank William Gray for providing material, Nataliia Gnyliukh and Ema Cervenova for help with manuscript preparation, and Julia Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at the Institute of Science and Technology Austria (ISTA) for their excellent service and assistance. The research leading to these results has received funding from the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; I 6123-B) to J.F., and Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by the FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985-2016). S.T. was supported by the National Natural Science Foundation of China (32321001, 32570366). The work of J.H. was supported by the project JG_2024_003 implemented within the Palacký University Young Researcher Grant.","corr_author":"1","author":[{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","first_name":"Lesia"},{"id":"7c417475-8972-11ed-ae7b-8b674ca26986","full_name":"Fiedler, Lukas","last_name":"Fiedler","first_name":"Lukas"},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","last_name":"Zou","first_name":"Minxia"},{"id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","last_name":"Giannini","full_name":"Giannini, Caterina","first_name":"Caterina"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","last_name":"Monzer","first_name":"Aline"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","first_name":"Dmitrii","last_name":"Vladimirtsev","full_name":"Vladimirtsev, Dmitrii"},{"first_name":"Marek","last_name":"Randuch","full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Yongfan","last_name":"Yu","full_name":"Yu, Yongfan"},{"first_name":"Zuzana","orcid":"0000-0003-4783-1752","full_name":"Gelová, Zuzana","last_name":"Gelová","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425"},{"orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","last_name":"Verstraeten","first_name":"Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87"},{"id":"4800CC20-F248-11E8-B48F-1D18A9856A87","first_name":"Jakub","full_name":"Hajny, Jakub","last_name":"Hajny","orcid":"0000-0003-2140-7195"},{"full_name":"Chen, Meng","last_name":"Chen","first_name":"Meng"},{"first_name":"Shutang","full_name":"Tan, Shutang","last_name":"Tan","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hörmayer, Lukas","last_name":"Hörmayer","orcid":"0000-0001-8295-2926","first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","first_name":"Lanxin","full_name":"Li, Lanxin","last_name":"Li","orcid":"0000-0002-5607-272X"},{"first_name":"Maria Mar","last_name":"Marques-Bueno","full_name":"Marques-Bueno, Maria Mar"},{"id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466","last_name":"Quddoos","full_name":"Quddoos, Zainab","first_name":"Zainab"},{"first_name":"Gergely","last_name":"Molnar","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87"},{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","full_name":"Kulich, Ivan","first_name":"Ivan"},{"full_name":"Jaillais, Yvon","last_name":"Jaillais","first_name":"Yvon"},{"first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"month":"10","PlanS_conform":"1","file_date_updated":"2025-11-24T10:55:18Z","doi":"10.1016/j.cell.2025.08.026","OA_place":"publisher","issue":"22","has_accepted_license":"1","day":"30","language":[{"iso":"eng"}],"article_processing_charge":"Yes (via OA deal)"},{"author":[{"full_name":"Rodriguez Solovey, Lesia","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","first_name":"Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Lukas","last_name":"Fiedler","full_name":"Fiedler, Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986"},{"first_name":"Minxia","full_name":"Zou, Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9"},{"id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","first_name":"Caterina","last_name":"Giannini","full_name":"Giannini, Caterina"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","last_name":"Monzer","first_name":"Aline"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"last_name":"Randuch","full_name":"Randuch, Marek","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Yongfan","last_name":"Yu","full_name":"Yu, Yongfan"},{"full_name":"Gelová, Zuzana","last_name":"Gelová","orcid":"0000-0003-4783-1752","first_name":"Zuzana","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425"},{"first_name":"Inge","orcid":"0000-0001-7241-2328","last_name":"Verstraeten","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87"},{"id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195","last_name":"Hajny","full_name":"Hajny, Jakub","first_name":"Jakub"},{"full_name":"Chen, Meng","last_name":"Chen","first_name":"Meng"},{"last_name":"Tan","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87"},{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","first_name":"Lukas","full_name":"Hörmayer, Lukas","last_name":"Hörmayer","orcid":"0000-0001-8295-2926"},{"id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5607-272X","last_name":"Li","full_name":"Li, Lanxin","first_name":"Lanxin"},{"last_name":"Marques-Bueno","full_name":"Marques-Bueno, Maria Mar","first_name":"Maria Mar"},{"last_name":"Quddoos","full_name":"Quddoos, Zainab","first_name":"Zainab","id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar","full_name":"Molnar, Gergely","first_name":"Gergely"},{"full_name":"Xu, Tongda","last_name":"Xu","first_name":"Tongda"},{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","full_name":"Kulich, Ivan","first_name":"Ivan"},{"first_name":"Yvon","last_name":"Jaillais","full_name":"Jaillais, Yvon"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"acknowledgement":"We thank W. Gray for providing material; N. Gnyliukh and E. Cervenova for help with manuscript preparation; J. Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at ISTA for their excellent service and assistance. The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program grant agreement No 742985 and Austrian Science Fund (FWF): I3630-775 B25 to J.F; National Natural Science Foundation of China (Grant 32130010, 31422008), start-up funds from FAFU to T.X., Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985- 2016). S.T. was supported by the National Natural Science Foundation of China (32321001).","corr_author":"1","department":[{"_id":"JiFr"},{"_id":"XiFe"}],"oa_version":"Published Version","status":"public","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","grant_number":"I03630"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734"},{"grant_number":"ALTF 985-2016","name":"Cell surface receptor complexes for auxin signaling in plants","_id":"26060676-B435-11E9-9278-68D0E5697425"}],"date_published":"2025-02-20T00:00:00Z","OA_type":"green","oa":1,"OA_place":"repository","language":[{"iso":"eng"}],"article_processing_charge":"No","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2022.11.30.518503"}],"day":"20","month":"02","das_tickbox":"1","doi":"10.1101/2022.11.30.518503","date_created":"2025-03-13T08:36:48Z","type":"preprint","ddc":["580"],"related_material":{"record":[{"status":"public","id":"20656","relation":"later_version"},{"status":"public","id":"20364","relation":"dissertation_contains"},{"status":"public","relation":"dissertation_contains","id":"19395"}]},"abstract":[{"lang":"eng","text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a key prerequisite for (i) self-organizing processes including vascular tissue formation and (ii) directional growth responses such as gravitropism. Here we identify a mechanism, by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface ABP1-TMK1 receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE auxin receptor ABL3. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably also for other self-organizing developmental phenomena."}],"publication_status":"draft","publication":"bioRxiv","date_updated":"2026-08-14T09:33:45Z","_id":"19399","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"citation":{"ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2022.11.30.518503\">10.1101/2022.11.30.518503</a>","short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, T. Xu, I. Kulich, Y. Jaillais, J. Friml, BioRxiv (n.d.).","ieee":"L. Rodriguez Solovey <i>et al.</i>, “ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism,” <i>bioRxiv</i>. .","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Xu T, Kulich I, Jaillais Y, Friml J. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. bioRxiv, <a href=\"https://doi.org/10.1101/2022.11.30.518503\">10.1101/2022.11.30.518503</a>.","mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2022.11.30.518503\">10.1101/2022.11.30.518503</a>.","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2022.11.30.518503\">https://doi.org/10.1101/2022.11.30.518503</a>.","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (n.d.). ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2022.11.30.518503\">https://doi.org/10.1101/2022.11.30.518503</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"title":"ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism","year":"2025"}]