[{"publisher":"Elsevier","article_number":"102487","external_id":{"pmid":["42214837"]},"publication_identifier":{"issn":["0959-437X"],"eissn":["1879-0380"]},"license":"https://creativecommons.org/licenses/by/4.0/","project":[{"name":"Role of cell lineage in generating cell-type diversity in developing neocortex’","_id":"7c084566-9f16-11ee-852c-c88a1dbbf1cf","grant_number":"ALTF 994-2023"},{"grant_number":"F7805","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"OA_type":"hybrid","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.gde.2026.102487"}],"year":"2026","abstract":[{"lang":"eng","text":"The cerebral cortex comprises diverse neuron and glial cell types generated by radial glial progenitors (RGPs) during development. Although RGPs broadly differentiate according to temporally and spatially regulated molecular logics, the lineage hierarchies linking individual progenitors to defined cell (sub)types are not well understood. Clone-resolved transcriptomics, combining molecular barcoding and single-cell RNA sequencing, allow high-resolution lineage tracing at the single-clone/cell level across different species and models. In this mini-review, we synthesize recent advances in this field, uncovering unexpected lineage relationships in the developing brain, with a particular focus on the cerebral cortex. We further highlight new insights into species-specific differences in the developmental programs generating cell-type diversity, linking changes in clonal architecture to lineage diversification during cortical evolution."}],"intvolume":"        99","oa":1,"pmid":1,"oa_version":"Published Version","_id":"21948","publication_status":"epub_ahead","title":"Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.gde.2026.102487","quality_controlled":"1","citation":{"ista":"Varela Martínez I, Pipicelli F, Hippenmeyer S. 2026. Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics. Current Opinion in Genetics &#38; Development. 99, 102487.","short":"I. Varela Martínez, F. Pipicelli, S. Hippenmeyer, Current Opinion in Genetics &#38; Development 99 (2026).","ieee":"I. Varela Martínez, F. Pipicelli, and S. Hippenmeyer, “Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics,” <i>Current Opinion in Genetics &#38; Development</i>, vol. 99. Elsevier, 2026.","apa":"Varela Martínez, I., Pipicelli, F., &#38; Hippenmeyer, S. (2026). Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics. <i>Current Opinion in Genetics &#38; Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.gde.2026.102487\">https://doi.org/10.1016/j.gde.2026.102487</a>","ama":"Varela Martínez I, Pipicelli F, Hippenmeyer S. Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics. <i>Current Opinion in Genetics &#38; Development</i>. 2026;99. doi:<a href=\"https://doi.org/10.1016/j.gde.2026.102487\">10.1016/j.gde.2026.102487</a>","mla":"Varela Martínez, Irene, et al. “Tracing Cell Lineages in the Developing Brain: Insights from Mosaic Analysis and Clone-Resolved Transcriptomics.” <i>Current Opinion in Genetics &#38; Development</i>, vol. 99, 102487, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.gde.2026.102487\">10.1016/j.gde.2026.102487</a>.","chicago":"Varela Martínez, Irene, Fabrizia Pipicelli, and Simon Hippenmeyer. “Tracing Cell Lineages in the Developing Brain: Insights from Mosaic Analysis and Clone-Resolved Transcriptomics.” <i>Current Opinion in Genetics &#38; Development</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.gde.2026.102487\">https://doi.org/10.1016/j.gde.2026.102487</a>."},"volume":99,"article_processing_charge":"Yes (via OA deal)","PlanS_conform":"1","scopus_import":"1","OA_place":"publisher","corr_author":"1","acknowledgement":"We wish to thank all members of the Hippenmeyer laboratory at ISTA for exciting discussions on the subject of this review. We apologize to colleagues whose work we could not cite and/or discuss in the frame of the available space. Work in the Hippenmeyer laboratory on the discussed topic is supported by ISTA institutional funds, an EMBO LTF (ALTF 994–2023) to F.P., FWF SFB F78 (10.55776/F78) to S.H., and FWF Cluster of Excellence COE16 (10.55776/COE16) to S.H.","article_type":"original","author":[{"full_name":"Varela Martínez, Irene","first_name":"Irene","id":"a69b5985-8829-11f0-8fc2-d0af58f64471","last_name":"Varela Martínez"},{"full_name":"Pipicelli, Fabrizia","first_name":"Fabrizia","last_name":"Pipicelli","id":"649134fd-d012-11ed-8f82-db1e5050f9ba"},{"orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon","first_name":"Simon","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2026-06-07T22:01:35Z","language":[{"iso":"eng"}],"day":"29","ddc":["570"],"date_published":"2026-05-29T00:00:00Z","month":"05","publication":"Current Opinion in Genetics & Development","status":"public","type":"journal_article","department":[{"_id":"SiHi"}],"has_accepted_license":"1","date_updated":"2026-08-12T09:56:19Z"},{"isi":1,"file":[{"success":1,"file_name":"2025_CurrentOpNeurobiology_Pipicelli.pdf","date_created":"2025-12-30T08:25:49Z","access_level":"open_access","relation":"main_file","file_size":1592649,"content_type":"application/pdf","file_id":"20894","date_updated":"2025-12-30T08:25:49Z","checksum":"05bacb4acbe6275d43e873dec9ba1d52","creator":"dernst"}],"article_processing_charge":"Yes (via OA deal)","PlanS_conform":"1","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.conb.2025.103046","quality_controlled":"1","citation":{"chicago":"Pipicelli, Fabrizia, Ana Villalba Requena, and Simon Hippenmeyer. “How Radial Glia Progenitor Lineages Generate Cell-Type Diversity in the Developing Cerebral Cortex.” <i>Current Opinion in Neurobiology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.conb.2025.103046\">https://doi.org/10.1016/j.conb.2025.103046</a>.","mla":"Pipicelli, Fabrizia, et al. “How Radial Glia Progenitor Lineages Generate Cell-Type Diversity in the Developing Cerebral Cortex.” <i>Current Opinion in Neurobiology</i>, vol. 93, 103046, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.conb.2025.103046\">10.1016/j.conb.2025.103046</a>.","ama":"Pipicelli F, Villalba Requena A, Hippenmeyer S. How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex. <i>Current Opinion in Neurobiology</i>. 2025;93. doi:<a href=\"https://doi.org/10.1016/j.conb.2025.103046\">10.1016/j.conb.2025.103046</a>","apa":"Pipicelli, F., Villalba Requena, A., &#38; Hippenmeyer, S. (2025). How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex. <i>Current Opinion in Neurobiology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.conb.2025.103046\">https://doi.org/10.1016/j.conb.2025.103046</a>","ieee":"F. Pipicelli, A. Villalba Requena, and S. Hippenmeyer, “How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex,” <i>Current Opinion in Neurobiology</i>, vol. 93. Elsevier, 2025.","short":"F. Pipicelli, A. Villalba Requena, S. Hippenmeyer, Current Opinion in Neurobiology 93 (2025).","ista":"Pipicelli F, Villalba Requena A, Hippenmeyer S. 2025. How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex. Current Opinion in Neurobiology. 93, 103046."},"volume":93,"status":"public","ddc":["570"],"date_published":"2025-08-01T00:00:00Z","month":"08","publication":"Current Opinion in Neurobiology","date_updated":"2025-12-30T10:54:14Z","type":"journal_article","has_accepted_license":"1","department":[{"_id":"SiHi"}],"acknowledgement":"We wish to thank all members of the Hippenmeyer laboratory at ISTA for exciting discussions on the subject of this review. We apologize to colleagues whose work we could not cite and/or discuss in the frame of the available space. Work in the Hippenmeyer laboratory on the discussed topic is supported by ISTA institutional funds, an EMBO LTF (ALTF 994–2023) to F.P, and FWF SFB F78 to S.H.","article_type":"original","corr_author":"1","OA_place":"publisher","language":[{"iso":"eng"}],"date_created":"2025-05-20T10:20:09Z","day":"01","file_date_updated":"2025-12-30T08:25:49Z","author":[{"full_name":"Pipicelli, Fabrizia","first_name":"Fabrizia","id":"649134fd-d012-11ed-8f82-db1e5050f9ba","last_name":"Pipicelli"},{"full_name":"Villalba Requena, Ana","orcid":"0000-0002-5615-5277","last_name":"Villalba Requena","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","first_name":"Ana"},{"first_name":"Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon"}],"publication_identifier":{"issn":["0959-4388"]},"external_id":{"pmid":["40383049"],"isi":["001496227000001"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"grant_number":"F7805","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"},{"name":"Role of cell lineage in generating cell-type diversity in developing neocortex’","_id":"7c084566-9f16-11ee-852c-c88a1dbbf1cf","grant_number":"ALTF 994-2023"}],"publisher":"Elsevier","article_number":"103046","pmid":1,"intvolume":"        93","oa":1,"_id":"19718","publication_status":"published","title":"How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex","oa_version":"Published Version","year":"2025","abstract":[{"text":"The cerebral cortex is arguably the most complex organ in humans. The cortical architecture is characterized by a remarkable diversity of neuronal and glial cell types that make up its neuronal circuits. Following a precise temporally ordered program, radial glia progenitor (RGP) cells generate all cortical excitatory projection neurons and glial cell-types. Cortical excitatory projection neurons are produced either directly or via intermediate progenitors, through indirect neurogenesis. How the extensive cortical cell-type diversity is generated during cortex development remains, however, a fundamental open question. How do RGPs quantitatively and qualitatively generate all the neocortical neurons? How does direct and indirect neurogenesis contribute to the establishment of neuronal and lineage heterogeneity? Whether RGPs represent a homogeneous and/or multipotent progenitor population, or if RGPs consist of heterogeneous groups is currently also not known. In this review, we will summarize the latest findings that contributed to a deeper insight into the above key questions.","lang":"eng"}],"OA_type":"hybrid"}]