[{"day":"11","year":"2026","language":[{"iso":"eng"}],"date_updated":"2026-02-23T11:03:33Z","title":"The splicing paralogues SNRPB and SNRPN control differential metabolic states.","date_published":"2026-02-11T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.02.11.705284"}],"publication":"bioRxiv","article_processing_charge":"No","citation":{"short":"F. Polat Haas, A. Villalba Requena, P. Rusina, A. Gopalan, H. Fritz, A. Akhmetkaliyev, F. Ruehle, A. Einsiedel, A. Szczepinska, F. Kielisch, J.-X. Chen, S. Nguyen, T. Schmidlin, S. Hippenmeyer, M.F. Bailicata, C.I. Keller Valsecchi, BioRxiv (n.d.).","mla":"Polat Haas, Feyza, et al. “The Splicing Paralogues SNRPB and SNRPN Control Differential Metabolic States.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.02.11.705284\">10.64898/2026.02.11.705284</a>.","ama":"Polat Haas F, Villalba Requena A, Rusina P, et al. The splicing paralogues SNRPB and SNRPN control differential metabolic states. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.02.11.705284\">10.64898/2026.02.11.705284</a>","ista":"Polat Haas F, Villalba Requena A, Rusina P, Gopalan A, Fritz H, Akhmetkaliyev A, Ruehle F, Einsiedel A, Szczepinska A, Kielisch F, Chen J-X, Nguyen S, Schmidlin T, Hippenmeyer S, Bailicata MF, Keller Valsecchi CI. The splicing paralogues SNRPB and SNRPN control differential metabolic states. bioRxiv, <a href=\"https://doi.org/10.64898/2026.02.11.705284\">10.64898/2026.02.11.705284</a>.","apa":"Polat Haas, F., Villalba Requena, A., Rusina, P., Gopalan, A., Fritz, H., Akhmetkaliyev, A., … Keller Valsecchi, C. I. (n.d.). The splicing paralogues SNRPB and SNRPN control differential metabolic states. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.02.11.705284\">https://doi.org/10.64898/2026.02.11.705284</a>","chicago":"Polat Haas, Feyza, Ana Villalba Requena, Polina Rusina, Anusha Gopalan, Hector Fritz, Azamat Akhmetkaliyev, Frank Ruehle, et al. “The Splicing Paralogues SNRPB and SNRPN Control Differential Metabolic States.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.02.11.705284\">https://doi.org/10.64898/2026.02.11.705284</a>.","ieee":"F. Polat Haas <i>et al.</i>, “The splicing paralogues SNRPB and SNRPN control differential metabolic states.,” <i>bioRxiv</i>. ."},"_id":"21290","oa_version":"Preprint","OA_type":"green","status":"public","month":"02","type":"preprint","fulldoi":"https://doi.org/10.64898/2026.02.11.705284","abstract":[{"lang":"eng","text":"Gene duplication underlies evolutionary innovation, yet many paralogues remain highly similar, raising questions about their functional divergence and physiological relevance. The spliceosomal Sm core protein SNRPB and its mammalian-specific paralogue SNRPN share over 90% sequence identity, but their distinct expression patterns - SNRPB being ubiquitous and SNRPN confined to the brain - suggest specialized functions. Why mammals have two different spliceosomes has remained obscure. Here, we generated isogenic human cell lines expressing ectopically either SNRPB or SNRPN exclusively and found that SNRPN stabilizes transcripts involved in energy metabolism and mitochondrial function, leading to increased mitochondrial abundance and oxygen consumption. Despite similar spliceosomal interactomes, SNRPN more strongly associates with the PRMT5 methylosome complex and exhibits dynamic arginine methylation in its C-terminal region that is sensitive to translation inhibition and amino acid availability. The SNRPN-dependent transcriptome responds to translation inhibition by stabilizing long, intron-rich genes involved in amino acid and energy metabolism. Our findings reveal a nutrient-sensitive, methylation-dependent mechanism that differentiates the two paralogues. This suggests that SNRPN functions as a metabolic-specialized spliceosomal subunit thereby providing tissue-specific adaptation of RNA processing in mammals."}],"date_created":"2026-02-17T11:35:59Z","doi":"10.64898/2026.02.11.705284","publication_status":"submitted","oa":1,"acknowledgement":"We thank Oliver Mühlemann and Alex Hofer (University of Bern) for sharing SMG inhibitors\r\nand for their expertise in nonsense-mediated mRNA decay and Maria Hondele for critical\r\nreading of the manuscript draft. We also thank the IMB Genomics Core Facility for assistance\r\nwith library preparation and sequencing, Martin Möckel and the IMB Protein Production Core\r\nFacility for providing enzymes used in this work, Marton Gelleri together with the IMB\r\nMicroscopy Core Facility for support with microscopy and FRAP experiments, Jasmin Cartano\r\nfor proteomics sample processing and the IMB Flow Cytometry Core Facility for support. In\r\naddition, we thank the Imaging Core Facility (IMCF) and the FACS Core Facility at the\r\nBiozentrum, University of Basel, for technical assistance. CIKV acknowledges funding by the\r\nDeutsche Forschungsgemeinschaft (DFG, German Research Foundation) - Individual Grant\r\nProject no. 513744403, Scientific Network Grant Project no. 531902894, GRK2526 “Genevo”\r\n- Project no. 407023052”, GRK2859 (“4R”) - Project no. 491145305, Forschungsinitiative\r\nRheinland-Pfalz (ReALity), the EMBO Young Investigator Program (5795), institutional\r\nfunding from the Institute of Molecular Biology and funds from the Kanton Basel-Stadt and\r\nBasel-Land provided to the Biozentrum of the University Basel. J.H.G.F.G. was part of the\r\n‘Science of Healthy Ageing Research Programme’ (SHARP) initiative funded by RhinelandPalatinate’s Ministry of Science, Education and Culture. PR is funded by the Biozentrum PhD\r\nFellowships Program. MFB received financial support from the intramural High Potentials\r\nGrant program of the University Medical Center Mainz, Forschungsinitiative Rheinland-Pfalz\r\n(ReALity) and Stiftungen zugunsten der Medizinischen Fakultät der LMU Klinikum (26069).\r\nInstruments in the IMB core facilities were supported by funds from the DFG: Laser Scanning\r\nConfocal (Leica Stellaris 8 Falcon, funded by the DFG - Project #497669232), Orbitrap Astral system (funded by the DFG - Project #524805621) and BD LSRFortessa SOPR is funded by\r\nthe DFG - Project #210253511.\r\n","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Polat Haas","first_name":"Feyza","full_name":"Polat Haas, Feyza"},{"orcid":"0000-0002-5615-5277","first_name":"Ana","full_name":"Villalba Requena, Ana","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","last_name":"Villalba Requena"},{"full_name":"Rusina, Polina","first_name":"Polina","last_name":"Rusina"},{"last_name":"Gopalan","full_name":"Gopalan, Anusha","first_name":"Anusha"},{"full_name":"Fritz, Hector","first_name":"Hector","last_name":"Fritz"},{"last_name":"Akhmetkaliyev","first_name":"Azamat","full_name":"Akhmetkaliyev, Azamat"},{"last_name":"Ruehle","full_name":"Ruehle, Frank","first_name":"Frank"},{"first_name":"Anna","full_name":"Einsiedel, Anna","last_name":"Einsiedel"},{"last_name":"Szczepinska","full_name":"Szczepinska, Anna","first_name":"Anna"},{"last_name":"Kielisch","first_name":"Fridolin","full_name":"Kielisch, Fridolin"},{"last_name":"Chen","first_name":"Jia-Xuan","full_name":"Chen, Jia-Xuan"},{"first_name":"Susanne","full_name":"Nguyen, Susanne","last_name":"Nguyen"},{"last_name":"Schmidlin","full_name":"Schmidlin, Thierry","first_name":"Thierry"},{"last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","first_name":"Simon"},{"full_name":"Bailicata, M. Felicia","first_name":"M. Felicia","last_name":"Bailicata"},{"last_name":"Keller Valsecchi","first_name":"Claudia Isabelle","full_name":"Keller Valsecchi, Claudia Isabelle"}],"department":[{"_id":"SiHi"}],"OA_place":"repository"},{"date_published":"2026-05-05T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.64898/2026.05.01.722172"}],"title":"Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner","citation":{"chicago":"Villalba Requena, Ana, Robert J Beattie, Florian Pauler, Carmen Streicher, Osvaldo Miranda, Thomas Krausgruber, Martin Senekowitsch, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>.","ieee":"A. Villalba Requena <i>et al.</i>, “Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner,” <i>bioRxiv</i>. .","short":"A. Villalba Requena, R.J. Beattie, F. Pauler, C. Streicher, O. Miranda, T. Krausgruber, M. Senekowitsch, M. Farlik, C. Bock, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","mla":"Villalba Requena, Ana, et al. “Mtor/Rptor Function Globally Prevents Cortical Microcephaly and Cell-Autonomously Promotes Postnatal Neuron Survival in Cell Type Specific Manner.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","ama":"Villalba Requena A, Beattie RJ, Pauler F, et al. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>","ista":"Villalba Requena A, Beattie RJ, Pauler F, Streicher C, Miranda O, Krausgruber T, Senekowitsch M, Farlik M, Bock C, Rülicke T, Hippenmeyer S. Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722172\">10.64898/2026.05.01.722172</a>.","apa":"Villalba Requena, A., Beattie, R. J., Pauler, F., Streicher, C., Miranda, O., Krausgruber, T., … Hippenmeyer, S. (n.d.). Mtor/Rptor function globally prevents cortical microcephaly and cell-autonomously promotes postnatal neuron survival in cell type specific manner. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722172\">https://doi.org/10.64898/2026.05.01.722172</a>"},"OA_type":"green","type":"preprint","month":"05","status":"public","year":"2026","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"MassSpec"},{"_id":"Bio"}],"date_updated":"2026-06-16T08:45:25Z","project":[{"name":"Molecular Mechanisms Regulating Gliogenesis in the Neocortex","_id":"264E56E2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"M02416"},{"grant_number":"618444","call_identifier":"FP7","_id":"25D61E48-B435-11E9-9278-68D0E5697425","name":"Molecular Mechanisms of Cerebral Cortex Development"},{"name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"725780"}],"acknowledgement":"We thank A. Heger (IST Austria Preclinical Facility), A. Sommer (VBCF GmbH, NGS Unit), and A.\r\nNicolas (IST Austria Lab Support Facility / Mass Spectrometry Facility) for technical support; K. Ferencak,\r\nI. Aykara, P. Hirschfeld, E. Fisher, S. Laukoter, L. Andersen for initial experiments and/or assistance; and\r\nall members of the Hippenmeyer lab for discussion. This research was supported by the Scientific Service\r\nUnits (SSU) of IST Austria through resources provided by the Imaging and Optics- (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). R.B. received support from FWF Meitner-Programm (M 2416). This\r\nwork was also supported by IST Austria institutional funds; the People Programme (Marie Curie Actions)\r\nof the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement\r\nNo 618444 to S.H., and the European Research Council (ERC) under the European Union’s Horizon 2020\r\nresearch and innovation programme (grant agreement No 725780 LinPro) to S.H.","department":[{"_id":"SiHi"}],"tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"abstract":[{"text":"The generation of faithful cell-type diversity and correct projection neuron numbers is essential for cerebral cortex development. Corticogenesis is however susceptible to genetic interference of critical signaling pathways, including mutations in Mtor/Rptor that lead to microcephaly. How the loss of Rptor/mTORC1 function affects cortical developmental programs, at single cell level, is still unknown. Here, we utilized Mosaic Analysis with Double Markers (MADM) technology to probe Rptor gene function upon sparse single cell- or global tissue-wide ablation. We found that tissue-wide effects drive the etiology of cortical microcephaly upon loss of Rptor, rather than deficits in projection neuron genesis. Conversely, Rptor function is cell-autonomously required for postnatal projection neuron survival in a highly cell-type-specific manner. Collectively, our results suggest that the fine balance of precise cell-type-specific cell-autonomous Rptor/mTORC1 function in concert with non-cell-autonomous tissue-wide effects is essential for the development of a properly-sized cerebral cortex with accurate projection neuron diversity.","lang":"eng"}],"fulldoi":"https://doi.org/10.64898/2026.05.01.722172","doi":"10.64898/2026.05.01.722172","publication_status":"submitted","publication":"bioRxiv","oa_version":"Preprint","_id":"21962","article_processing_charge":"No","day":"05","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"author":[{"id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","last_name":"Villalba Requena","first_name":"Ana","orcid":"0000-0002-5615-5277","full_name":"Villalba Requena, Ana"},{"orcid":"0000-0002-8483-8753","first_name":"Robert J","full_name":"Beattie, Robert J","id":"2E26DF60-F248-11E8-B48F-1D18A9856A87","last_name":"Beattie"},{"last_name":"Pauler","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","full_name":"Pauler, Florian","first_name":"Florian","orcid":"0000-0002-7462-0048"},{"full_name":"Streicher, Carmen","first_name":"Carmen","last_name":"Streicher","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Miranda","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425","full_name":"Miranda, Osvaldo","first_name":"Osvaldo","orcid":"0000-0001-6618-6889"},{"full_name":"Krausgruber, Thomas","first_name":"Thomas","last_name":"Krausgruber"},{"last_name":"Senekowitsch","full_name":"Senekowitsch, Martin","first_name":"Martin"},{"full_name":"Farlik, Matthias","first_name":"Matthias","last_name":"Farlik"},{"last_name":"Bock","first_name":"Christoph","full_name":"Bock, Christoph"},{"last_name":"Rülicke","first_name":"Thomas","full_name":"Rülicke, Thomas"},{"orcid":"0000-0003-2279-1061","first_name":"Simon","full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer"}],"ddc":["570"],"OA_place":"repository","date_created":"2026-06-09T08:08:18Z","oa":1,"has_accepted_license":"1"},{"year":"2026","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"date_updated":"2026-06-16T08:57:20Z","main_file_link":[{"url":"https://doi.org/10.64898/2026.05.01.722191","open_access":"1"}],"date_published":"2026-05-05T00:00:00Z","title":"Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production","citation":{"mla":"Miranda, Osvaldo, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","short":"O. Miranda, X. Contreras, F. Pauler, A. Davaatseren, N. Amberg, C. Streicher, A. Villalba Requena, A.-M. Heger, C. Marie, B.A. Hassan, T. Rülicke, S. Hippenmeyer, BioRxiv (n.d.).","apa":"Miranda, O., Contreras, X., Pauler, F., Davaatseren, A., Amberg, N., Streicher, C., … Hippenmeyer, S. (n.d.). Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>","ama":"Miranda O, Contreras X, Pauler F, et al. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>","ista":"Miranda O, Contreras X, Pauler F, Davaatseren A, Amberg N, Streicher C, Villalba Requena A, Heger A-M, Marie C, Hassan BA, Rülicke T, Hippenmeyer S. Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production. bioRxiv, <a href=\"https://doi.org/10.64898/2026.05.01.722191\">10.64898/2026.05.01.722191</a>.","ieee":"O. Miranda <i>et al.</i>, “Pten orchestrates neurogenic radial glia lineage progression and tunes neocortical astrocyte production,” <i>bioRxiv</i>. .","chicago":"Miranda, Osvaldo, Ximena Contreras, Florian Pauler, Amarbayasgalan Davaatseren, Nicole Amberg, Carmen Streicher, Ana Villalba Requena, et al. “Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.05.01.722191\">https://doi.org/10.64898/2026.05.01.722191</a>."},"OA_type":"green","month":"05","type":"preprint","status":"public","tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"abstract":[{"text":"The cerebral cortex consists of immense numbers of neuronal and glial cell-types derived from radial glial progenitor (RGP) cells. How RGPs generate appropriate quantities of distinct cortical cell-types to safeguard a brain of correct size, is not well understood. However, genetic aberration in human, including mutations in PTEN, lead to cortical malformation such as macrocephaly, albeit with unknown etiology. Here we utilized Mosaic Analysis with Double Markers (MADM)-based clonal analysis and single cell phenotyping to decipher the role of Pten in neurogenic and gliogenic RGP lineage progression during cortical ontogeny. While neurogenic RGP lineage progression and projection neuron production was moderately altered in the absence of Pten, cortical astrocyte production was drastically increased. Through genetic epistasis experiments we show that the loss of Pten uncouples astrocyte generation from essential growth factor signaling hubs, funneling into MAPK. Collectively, our results suggest that Pten regulates RGP lineage progression with distinct sequential functions in cortical projection neurogenesis and astrocyte production to ensure the emergence of a correctly-sized cerebral cortex.","lang":"eng"}],"fulldoi":"https://doi.org/10.64898/2026.05.01.722191","doi":"10.64898/2026.05.01.722191","publication_status":"submitted","acknowledgement":"We thank Kay-Uwe Wagner (Wayne State University) for generously sharing Jak1/2–flox mouse lines; A.\r\nSommer (VBCF GmbH, NGS Unit) for technical support; N. Kim, V. Mick, S. Schnabl, S. Gobeil, and L.\r\nAndersen for technical assistance; all members of the Hippenmeyer lab for discussion and B. Novitch for\r\ncomments on earlier versions of the manuscript. This research was supported by the Scientific Service Units\r\n(SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF), Lab Support-\r\n(LSF) and Preclinical Facilities (PCF). O.A.M received support from the Austrian Academy of Sciences\r\nÖAW (DOC 186584), and N.A. from FWF Elise Richter Program (Grant V1041T). This work was also\r\nsupported by IST Austria institutional funds; FWF SFB F78 (Neuro Stem Modulation) to S.H., and the\r\nEuropean Research Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogramme (grant agreement No 725780 LinPro) to S.H.","project":[{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805"},{"name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"725780"}],"department":[{"_id":"SiHi"},{"_id":"PreCl"},{"_id":"GradSch"}],"day":"05","language":[{"iso":"eng"}],"publication":"bioRxiv","_id":"21963","oa_version":"Preprint","article_processing_charge":"No","corr_author":"1","date_created":"2026-06-09T08:08:53Z","oa":1,"has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"author":[{"last_name":"Miranda","id":"862A3C56-A8BF-11E9-B4FA-D9E3E5697425","full_name":"Miranda, Osvaldo","orcid":"0000-0001-6618-6889","first_name":"Osvaldo"},{"full_name":"Contreras, Ximena","first_name":"Ximena","last_name":"Contreras","id":"475990FE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Pauler","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","full_name":"Pauler, Florian","orcid":"0000-0002-7462-0048","first_name":"Florian"},{"last_name":"Davaatseren","id":"70ADC922-B424-11E9-99E3-BA18E6697425","full_name":"Davaatseren, Amarbayasgalan","first_name":"Amarbayasgalan"},{"id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg","first_name":"Nicole","orcid":"0000-0002-3183-8207","full_name":"Amberg, Nicole"},{"full_name":"Streicher, Carmen","first_name":"Carmen","last_name":"Streicher","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87"},{"id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","last_name":"Villalba Requena","orcid":"0000-0002-5615-5277","first_name":"Ana","full_name":"Villalba Requena, Ana"},{"full_name":"Heger, Anna-Magdalena","first_name":"Anna-Magdalena","last_name":"Heger","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Marie, Corentine","first_name":"Corentine","last_name":"Marie"},{"full_name":"Hassan, Bassem A.","first_name":"Bassem A.","last_name":"Hassan"},{"last_name":"Rülicke","full_name":"Rülicke, Thomas","first_name":"Thomas"},{"last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","first_name":"Simon","orcid":"0000-0003-2279-1061"}],"ddc":["570"],"OA_place":"repository"},{"date_created":"2026-08-16T22:01:43Z","DOAJ_listed":"1","PlanS_conform":"1","quality_controlled":"1","has_accepted_license":"1","oa":1,"author":[{"last_name":"Varela Martínez","id":"a69b5985-8829-11f0-8fc2-d0af58f64471","full_name":"Varela Martínez, Irene","first_name":"Irene"},{"id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","last_name":"Villalba Requena","first_name":"Ana","orcid":"0000-0002-5615-5277","full_name":"Villalba Requena, Ana"},{"full_name":"García-Marqués, Jorge","first_name":"Jorge","last_name":"García-Marqués"},{"last_name":"Aguilera","first_name":"Alfonso","full_name":"Aguilera, Alfonso"},{"full_name":"Castro, Diogo S.","first_name":"Diogo S.","last_name":"Castro"},{"last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","first_name":"Simon","orcid":"0000-0003-2279-1061"},{"last_name":"Nieto","first_name":"Marta","full_name":"Nieto, Marta"}],"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","OA_place":"publisher","ddc":["570"],"file":[{"relation":"main_file","date_created":"2026-08-20T05:39:32Z","file_name":"2026_ScienceAdv_VarelaMartinez.pdf","file_id":"22738","success":1,"date_updated":"2026-08-20T05:39:32Z","content_type":"application/pdf","file_size":3056744,"access_level":"open_access","checksum":"487c3703387080e8f3c4675d67763f0e","creator":"dernst"}],"day":"07","das_tickbox":"1","language":[{"iso":"eng"}],"article_processing_charge":"Yes","_id":"22714","publisher":"AAAS","oa_version":"Published Version","publication":"Science Advances","researchdata_availability":"yes","doi":"10.1126/sciadv.adw5487","scopus_import":"1","fulldoi":"https://doi.org/10.1126/sciadv.adw5487","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"text":"Radial glial progenitors (RGPs) generate all projection neurons (PNs) in the cerebral cortex through incompletely understood processes. We combined Mosaic Analysis with Double Markers at embryonic stages (E)12.5 and E13.5 with early postnatal callosal tracing to dissect RGP lineage progression. We find that multipotent RGPs generate all extra-telencephalic (ET) and intra-telencephalic (IT) PNs via parallel sublineages that emerge simultaneously at neurogenesis onset. ET-PN production progresses exclusively via small, self-consuming lineages; IT-PN lineages feature RGPs generating large translaminar outputs. The early emergence of IT-PN–fated RGPs, coinciding with a switch to direct neurogenesis, contributes to the stereotyped population-level progression of the multipotent lineage. We also identify POU3F transcription factors as candidate regulators of IT-PN fate via noncanonical mitotic chromatin binding. The results support a model whereby IT- and ET-PNs arise from an early bifurcation and parallel specification within the multipotent RGP lineage.","lang":"eng"}],"external_id":{"pmid":["42555737"]},"publication_status":"published","acknowledgement":"We thank M. caouyette for the plasmid construction for Pou3f1overexpression; d. Pinto-Benito for valuable assistance with shRnA validation in n2A cells andqPcR experiments; c. Varela-Martínez for help with the code for graphical analysis; allmembers from the nieto’s lab for comment on the manuscript, specially to F. Martín for theinsightful discussions; J. c. Oliveros and J. A. García from the computational service of the cnBfor help with the analysis of RnAseq dataset; c. O. Sorzano for help with statistical analysis; andA. Oña and the service of Advance Optical Microscopy of the cnB for technical advice.Funding: i.V.-M. holds a fellowship funded by MciciU (PRe-2018-083376) and 2023 eMBOscientific exchange grant 10214. the work was funded by grants to M.n. (Pid2020-112831GB- i00 and Pid2023-146322nB- i00 by Mcin/Aei/10.13039/501100011033 and by“eRdF A way of making europe”).","department":[{"_id":"SiHi"}],"issue":"32","year":"2026","page":"eadw5487","volume":12,"publication_identifier":{"eissn":["2375-2548"]},"supplementarymaterial":"yes","date_updated":"2026-08-20T05:45:28Z","file_date_updated":"2026-08-20T05:39:32Z","citation":{"mla":"Varela Martínez, Irene, et al. “Early Fate Diversification of Radial Glial Progenitors during Corticogenesis.” <i>Science Advances</i>, vol. 12, no. 32, AAAS, 2026, p. eadw5487, doi:<a href=\"https://doi.org/10.1126/sciadv.adw5487\">10.1126/sciadv.adw5487</a>.","short":"I. Varela Martínez, A. Villalba Requena, J. García-Marqués, A. Aguilera, D.S. Castro, S. Hippenmeyer, M. Nieto, Science Advances 12 (2026) eadw5487.","ista":"Varela Martínez I, Villalba Requena A, García-Marqués J, Aguilera A, Castro DS, Hippenmeyer S, Nieto M. 2026. Early fate diversification of radial glial progenitors during corticogenesis. Science Advances. 12(32), eadw5487.","ama":"Varela Martínez I, Villalba Requena A, García-Marqués J, et al. Early fate diversification of radial glial progenitors during corticogenesis. <i>Science Advances</i>. 2026;12(32):eadw5487. doi:<a href=\"https://doi.org/10.1126/sciadv.adw5487\">10.1126/sciadv.adw5487</a>","apa":"Varela Martínez, I., Villalba Requena, A., García-Marqués, J., Aguilera, A., Castro, D. S., Hippenmeyer, S., &#38; Nieto, M. (2026). Early fate diversification of radial glial progenitors during corticogenesis. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.adw5487\">https://doi.org/10.1126/sciadv.adw5487</a>","ieee":"I. Varela Martínez <i>et al.</i>, “Early fate diversification of radial glial progenitors during corticogenesis,” <i>Science Advances</i>, vol. 12, no. 32. AAAS, p. eadw5487, 2026.","chicago":"Varela Martínez, Irene, Ana Villalba Requena, Jorge García-Marqués, Alfonso Aguilera, Diogo S. Castro, Simon Hippenmeyer, and Marta Nieto. “Early Fate Diversification of Radial Glial Progenitors during Corticogenesis.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.adw5487\">https://doi.org/10.1126/sciadv.adw5487</a>."},"intvolume":"        12","dataavailabilitystatement":"All data and code needed to evaluate and reproduce the results in the paper are present in the paper and/or the Supplementary Materials. Source data underlying all figures (including the clonal atlas) are provided in the Supplementary Materials. RnA-seq analysis code has been deposited in Zenodo (dOi: 10.5281/zenodo.14609057). this study did not generate new materials.","title":"Early fate diversification of radial glial progenitors during corticogenesis","date_published":"2026-08-07T00:00:00Z","status":"public","type":"journal_article","month":"08","OA_type":"gold"},{"day":"01","language":[{"iso":"eng"}],"publication":"Current Opinion in Neurobiology","_id":"19718","oa_version":"Published Version","publisher":"Elsevier","article_processing_charge":"Yes (via OA deal)","corr_author":"1","article_number":"103046","PlanS_conform":"1","date_created":"2025-05-20T10:20:09Z","oa":1,"has_accepted_license":"1","quality_controlled":"1","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Pipicelli, Fabrizia","first_name":"Fabrizia","last_name":"Pipicelli","id":"649134fd-d012-11ed-8f82-db1e5050f9ba"},{"last_name":"Villalba Requena","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","full_name":"Villalba Requena, Ana","first_name":"Ana","orcid":"0000-0002-5615-5277"},{"id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer","first_name":"Simon","orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon"}],"isi":1,"file":[{"file_name":"2025_CurrentOpNeurobiology_Pipicelli.pdf","date_created":"2025-12-30T08:25:49Z","relation":"main_file","date_updated":"2025-12-30T08:25:49Z","content_type":"application/pdf","success":1,"file_id":"20894","access_level":"open_access","file_size":1592649,"creator":"dernst","checksum":"05bacb4acbe6275d43e873dec9ba1d52"}],"ddc":["570"],"article_type":"original","OA_place":"publisher","publication_identifier":{"issn":["0959-4388"]},"volume":93,"year":"2025","file_date_updated":"2025-12-30T08:25:49Z","date_updated":"2025-12-30T10:54:14Z","date_published":"2025-08-01T00:00:00Z","title":"How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex","intvolume":"        93","citation":{"short":"F. Pipicelli, A. Villalba Requena, S. Hippenmeyer, Current Opinion in Neurobiology 93 (2025).","mla":"Pipicelli, Fabrizia, et al. “How Radial Glia Progenitor Lineages Generate Cell-Type Diversity in the Developing Cerebral Cortex.” <i>Current Opinion in Neurobiology</i>, vol. 93, 103046, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.conb.2025.103046\">10.1016/j.conb.2025.103046</a>.","apa":"Pipicelli, F., Villalba Requena, A., &#38; Hippenmeyer, S. (2025). How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex. <i>Current Opinion in Neurobiology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.conb.2025.103046\">https://doi.org/10.1016/j.conb.2025.103046</a>","ista":"Pipicelli F, Villalba Requena A, Hippenmeyer S. 2025. How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex. Current Opinion in Neurobiology. 93, 103046.","ama":"Pipicelli F, Villalba Requena A, Hippenmeyer S. How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex. <i>Current Opinion in Neurobiology</i>. 2025;93. doi:<a href=\"https://doi.org/10.1016/j.conb.2025.103046\">10.1016/j.conb.2025.103046</a>","chicago":"Pipicelli, Fabrizia, Ana Villalba Requena, and Simon Hippenmeyer. “How Radial Glia Progenitor Lineages Generate Cell-Type Diversity in the Developing Cerebral Cortex.” <i>Current Opinion in Neurobiology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.conb.2025.103046\">https://doi.org/10.1016/j.conb.2025.103046</a>.","ieee":"F. Pipicelli, A. Villalba Requena, and S. Hippenmeyer, “How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex,” <i>Current Opinion in Neurobiology</i>, vol. 93. Elsevier, 2025."},"OA_type":"hybrid","type":"journal_article","month":"08","status":"public","abstract":[{"lang":"eng","text":"The cerebral cortex is arguably the most complex organ in humans. The cortical architecture is characterized by a remarkable diversity of neuronal and glial cell types that make up its neuronal circuits. Following a precise temporally ordered program, radial glia progenitor (RGP) cells generate all cortical excitatory projection neurons and glial cell-types. Cortical excitatory projection neurons are produced either directly or via intermediate progenitors, through indirect neurogenesis. How the extensive cortical cell-type diversity is generated during cortex development remains, however, a fundamental open question. How do RGPs quantitatively and qualitatively generate all the neocortical neurons? How does direct and indirect neurogenesis contribute to the establishment of neuronal and lineage heterogeneity? Whether RGPs represent a homogeneous and/or multipotent progenitor population, or if RGPs consist of heterogeneous groups is currently also not known. In this review, we will summarize the latest findings that contributed to a deeper insight into the above key questions."}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"fulldoi":"https://doi.org/10.1016/j.conb.2025.103046","scopus_import":"1","doi":"10.1016/j.conb.2025.103046","publication_status":"published","external_id":{"pmid":["40383049"],"isi":["001496227000001"]},"project":[{"_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805","name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression"},{"name":"Role of cell lineage in generating cell-type diversity in developing neocortex’","grant_number":"ALTF 994-2023","_id":"7c084566-9f16-11ee-852c-c88a1dbbf1cf"}],"acknowledgement":"We wish to thank all members of the Hippenmeyer laboratory at ISTA for exciting discussions on the subject of this review. We apologize to colleagues whose work we could not cite and/or discuss in the frame of the available space. Work in the Hippenmeyer laboratory on the discussed topic is supported by ISTA institutional funds, an EMBO LTF (ALTF 994–2023) to F.P, and FWF SFB F78 to S.H.","department":[{"_id":"SiHi"}]},{"acknowledgement":"We thank M. Caouyette for the plasmid construction for Pou3f1 overexpression; C. Varela747 Martínez for help with the code for graphical analysis; all members from the Nieto’s lab for\r\ncomment on the manuscript, specially to F. Martín for the insightful discussions;J.C. Oliveros\r\nand J.A. García from the computational service of the CNB for help with the analysis of\r\nRNAseq dataset, C.O. Sorzano for the help with statistical analysis, and the service of\r\nAdvance Optical Microscopy of the CNB for their technical advice.\r\nI.V.M holds a fellowship funded by MCICIU (PRE-2018-083376), the work was funded by\r\nPID2020-112831GB-I00 funded by MCIN/AEI /10.13039/501100011033.\r\n","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Varela-Martínez, I","first_name":"I","last_name":"Varela-Martínez"},{"orcid":"0000-0002-5615-5277","first_name":"Ana","full_name":"Villalba Requena, Ana","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","last_name":"Villalba Requena"},{"first_name":"J.","full_name":"Garcia-Marqués, J.","last_name":"Garcia-Marqués"},{"last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","first_name":"Simon"},{"first_name":"M.","full_name":"Nieto, M.","last_name":"Nieto"}],"department":[{"_id":"SiHi"}],"OA_place":"repository","fulldoi":"https://doi.org/10.1101/2025.05.07.652665","abstract":[{"lang":"eng","text":"Radial glial progenitors (RGPs) generate all projection neurons (PNs) in the cerebral cortex through incompletely understood processes. Herein, we combine Mosaic Analysis with Double Markers (MADM)-based clonal analysis at embryonic days 12.5 and 13.5 with early postnatal callosal tracing to reveal a lineage progression that challenges the inside-outside model of cortical development and the conventional view of an invariable sequence of asymmetric neurogenic divisions. Our data demonstrate that early multipotent RGPs generate all extra-telencephalic (ET) and intra-telencephalic (IT) PNs across all layers through parallel sublineages and the random specification, during the earliest neurogenic divisions, of fate-restricted daughter RGPs. While the neuronal production of the parental multipotent RGPs consists of small ET-PN or IT-PN outputs, fate-restricted RGPs produce larger translaminar outputs spanning deep and upper layers of only IT-PNs, the predominant mammalian PN subtype. We further show that the emergence of IT-PN fate-restricted RGPs also leads to quantitatively and temporally stereotyped neurogenesis population-wise."}],"date_created":"2025-05-20T10:19:29Z","doi":"10.1101/2025.05.07.652665","publication_status":"submitted","oa":1,"title":"Early emergence of projection-subtype fate-restricted radial glial progenitors orchestrates neocortical neurogenesis","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.07.652665"}],"date_published":"2025-05-07T00:00:00Z","publication":"bioRxiv","article_processing_charge":"No","citation":{"mla":"Varela-Martínez, I., et al. “Early Emergence of Projection-Subtype Fate-Restricted Radial Glial Progenitors Orchestrates Neocortical Neurogenesis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.05.07.652665\">10.1101/2025.05.07.652665</a>.","short":"I. Varela-Martínez, A. Villalba Requena, J. Garcia-Marqués, S. Hippenmeyer, M. Nieto, BioRxiv (n.d.).","ama":"Varela-Martínez I, Villalba Requena A, Garcia-Marqués J, Hippenmeyer S, Nieto M. Early emergence of projection-subtype fate-restricted radial glial progenitors orchestrates neocortical neurogenesis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.05.07.652665\">10.1101/2025.05.07.652665</a>","apa":"Varela-Martínez, I., Villalba Requena, A., Garcia-Marqués, J., Hippenmeyer, S., &#38; Nieto, M. (n.d.). Early emergence of projection-subtype fate-restricted radial glial progenitors orchestrates neocortical neurogenesis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.05.07.652665\">https://doi.org/10.1101/2025.05.07.652665</a>","ista":"Varela-Martínez I, Villalba Requena A, Garcia-Marqués J, Hippenmeyer S, Nieto M. Early emergence of projection-subtype fate-restricted radial glial progenitors orchestrates neocortical neurogenesis. bioRxiv, <a href=\"https://doi.org/10.1101/2025.05.07.652665\">10.1101/2025.05.07.652665</a>.","ieee":"I. Varela-Martínez, A. Villalba Requena, J. Garcia-Marqués, S. Hippenmeyer, and M. Nieto, “Early emergence of projection-subtype fate-restricted radial glial progenitors orchestrates neocortical neurogenesis,” <i>bioRxiv</i>. .","chicago":"Varela-Martínez, I, Ana Villalba Requena, J. Garcia-Marqués, Simon Hippenmeyer, and M. Nieto. “Early Emergence of Projection-Subtype Fate-Restricted Radial Glial Progenitors Orchestrates Neocortical Neurogenesis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.05.07.652665\">https://doi.org/10.1101/2025.05.07.652665</a>."},"_id":"19717","oa_version":"Preprint","OA_type":"green","status":"public","type":"preprint","month":"05","day":"07","year":"2025","language":[{"iso":"eng"}],"date_updated":"2026-08-13T07:16:48Z"},{"language":[{"iso":"eng"}],"day":"01","corr_author":"1","oa_version":"Published Version","_id":"12542","publisher":"Elsevier","article_processing_charge":"No","publication":"Neuron","quality_controlled":"1","oa":1,"date_created":"2023-02-12T23:00:58Z","ddc":["570"],"article_type":"letter_note","OA_place":"publisher","isi":1,"author":[{"last_name":"Villalba Requena","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","full_name":"Villalba Requena, Ana","first_name":"Ana","orcid":"0000-0002-5615-5277"},{"last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","first_name":"Simon"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"date_updated":"2026-06-18T17:27:44Z","page":"291-293","year":"2023","issue":"3","publication_identifier":{"eissn":["1097-4199"]},"volume":111,"month":"02","type":"journal_article","status":"public","OA_type":"free access","intvolume":"       111","citation":{"chicago":"Villalba Requena, Ana, and Simon Hippenmeyer. “Going Back in Time with TEMPO.” <i>Neuron</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.neuron.2023.01.006\">https://doi.org/10.1016/j.neuron.2023.01.006</a>.","ieee":"A. Villalba Requena and S. Hippenmeyer, “Going back in time with TEMPO,” <i>Neuron</i>, vol. 111, no. 3. Elsevier, pp. 291–293, 2023.","ama":"Villalba Requena A, Hippenmeyer S. Going back in time with TEMPO. <i>Neuron</i>. 2023;111(3):291-293. doi:<a href=\"https://doi.org/10.1016/j.neuron.2023.01.006\">10.1016/j.neuron.2023.01.006</a>","ista":"Villalba Requena A, Hippenmeyer S. 2023. Going back in time with TEMPO. Neuron. 111(3), 291–293.","apa":"Villalba Requena, A., &#38; Hippenmeyer, S. (2023). Going back in time with TEMPO. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2023.01.006\">https://doi.org/10.1016/j.neuron.2023.01.006</a>","short":"A. Villalba Requena, S. Hippenmeyer, Neuron 111 (2023) 291–293.","mla":"Villalba Requena, Ana, and Simon Hippenmeyer. “Going Back in Time with TEMPO.” <i>Neuron</i>, vol. 111, no. 3, Elsevier, 2023, pp. 291–93, doi:<a href=\"https://doi.org/10.1016/j.neuron.2023.01.006\">10.1016/j.neuron.2023.01.006</a>."},"date_published":"2023-02-01T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.neuron.2023.01.006"}],"title":"Going back in time with TEMPO","publication_status":"published","external_id":{"isi":["000994473300001"],"pmid":["36731425"]},"doi":"10.1016/j.neuron.2023.01.006","abstract":[{"text":"In this issue of Neuron, Espinosa-Medina et al.1 present the TEMPO (Temporal Encoding and Manipulation in a Predefined Order) system, which enables the marking and genetic manipulation of sequentially generated cell lineages in vertebrate species in vivo.","lang":"eng"}],"scopus_import":"1","fulldoi":"https://doi.org/10.1016/j.neuron.2023.01.006","department":[{"_id":"SiHi"}]},{"corr_author":"1","status":"public","type":"book_chapter","month":"08","title":"Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression","date_published":"2023-08-08T00:00:00Z","publication":"Neocortical Neurogenesis in Development and Evolution","article_processing_charge":"No","citation":{"ama":"Villalba Requena A, Amberg N, Hippenmeyer S. Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression. In: Huttner W, ed. <i>Neocortical Neurogenesis in Development and Evolution</i>. Wiley; 2023:169-191. doi:<a href=\"https://doi.org/10.1002/9781119860914.ch10\">10.1002/9781119860914.ch10</a>","apa":"Villalba Requena, A., Amberg, N., &#38; Hippenmeyer, S. (2023). Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression. In W. Huttner (Ed.), <i>Neocortical Neurogenesis in Development and Evolution</i> (pp. 169–191). Wiley. <a href=\"https://doi.org/10.1002/9781119860914.ch10\">https://doi.org/10.1002/9781119860914.ch10</a>","ista":"Villalba Requena A, Amberg N, Hippenmeyer S. 2023.Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression. In: Neocortical Neurogenesis in Development and Evolution. , 169–191.","mla":"Villalba Requena, Ana, et al. “Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression.” <i>Neocortical Neurogenesis in Development and Evolution</i>, edited by Wieland Huttner, Wiley, 2023, pp. 169–91, doi:<a href=\"https://doi.org/10.1002/9781119860914.ch10\">10.1002/9781119860914.ch10</a>.","short":"A. Villalba Requena, N. Amberg, S. Hippenmeyer, in:, W. Huttner (Ed.), Neocortical Neurogenesis in Development and Evolution, Wiley, 2023, pp. 169–191.","ieee":"A. Villalba Requena, N. Amberg, and S. Hippenmeyer, “Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression,” in <i>Neocortical Neurogenesis in Development and Evolution</i>, W. Huttner, Ed. Wiley, 2023, pp. 169–191.","chicago":"Villalba Requena, Ana, Nicole Amberg, and Simon Hippenmeyer. “Interplay of Cell‐autonomous Gene Function and Tissue‐wide Mechanisms Regulating Radial Glial Progenitor Lineage Progression.” In <i>Neocortical Neurogenesis in Development and Evolution</i>, edited by Wieland Huttner, 169–91. Wiley, 2023. <a href=\"https://doi.org/10.1002/9781119860914.ch10\">https://doi.org/10.1002/9781119860914.ch10</a>."},"_id":"14757","publisher":"Wiley","oa_version":"None","language":[{"iso":"eng"}],"date_updated":"2024-10-09T21:07:46Z","day":"08","publication_identifier":{"eisbn":["9781119860914"]},"year":"2023","page":"169-191","department":[{"_id":"SiHi"}],"editor":[{"last_name":"Huttner","full_name":"Huttner, Wieland","first_name":"Wieland"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Villalba Requena","id":"68cb85a0-39f7-11eb-9559-9aaab4f6a247","full_name":"Villalba Requena, Ana","orcid":"0000-0002-5615-5277","first_name":"Ana"},{"id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg","first_name":"Nicole","orcid":"0000-0002-3183-8207","full_name":"Amberg, Nicole"},{"first_name":"Simon","orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","last_name":"Hippenmeyer"}],"publication_status":"published","quality_controlled":"1","fulldoi":"https://doi.org/10.1002/9781119860914.ch10","scopus_import":"1","abstract":[{"text":"The cerebral cortex is comprised of a vast cell-type diversity sequentially generated by cortical progenitor cells. Faithful progenitor lineage progression requires the tight orchestration of distinct molecular and cellular mechanisms regulating proper progenitor proliferation behavior and differentiation. Correct execution of developmental programs involves a complex interplay of cell intrinsic and tissue-wide mechanisms. Many studies over the past decades have been able to determine a plethora of genes critically involved in cortical development. However, only a few made use of genetic paradigms with sparse and global gene deletion to probe cell-autonomous vs. tissue-wide contribution. In this chapter, we will elaborate on the importance of dissecting the cell-autonomous and tissue-wide mechanisms to gain a precise understanding of gene function during radial glial progenitor lineage progression.","lang":"eng"}],"date_created":"2024-01-08T13:16:36Z","doi":"10.1002/9781119860914.ch10"}]