[{"external_id":{"pmid":["41990180"]},"publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"date_published":"2026-04-16T00:00:00Z","article_type":"original","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"file_date_updated":"2026-05-07T05:54:43Z","doi":"10.1126/science.adu8197","OA_place":"repository","page":"296-300","author":[{"last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","full_name":"Kulich, Ivan","first_name":"Ivan"},{"full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"full_name":"Randuch, Marek","first_name":"Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"full_name":"Gao, Shiqiang","first_name":"Shiqiang","last_name":"Gao"},{"last_name":"Citterico","first_name":"Matteo","full_name":"Citterico, Matteo"},{"first_name":"Kai R.","full_name":"Konrad, Kai R.","last_name":"Konrad"},{"first_name":"Georg","full_name":"Nagel, Georg","last_name":"Nagel"},{"last_name":"Wrzaczek","first_name":"Michael","full_name":"Wrzaczek, Michael"},{"full_name":"Cascaro, Léa","first_name":"Léa","last_name":"Cascaro"},{"full_name":"Vinet, Pauline","first_name":"Pauline","last_name":"Vinet"},{"first_name":"Pauline","full_name":"Durand, Pauline","last_name":"Durand"},{"last_name":"Asnacios","first_name":"Atef","full_name":"Asnacios, Atef"},{"full_name":"Verma, Lokesh","first_name":"Lokesh","last_name":"Verma"},{"first_name":"Malcolm J.","full_name":"Bennett, Malcolm J.","last_name":"Bennett"},{"full_name":"Pandey, Bipin K.","first_name":"Bipin K.","last_name":"Pandey"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"citation":{"ieee":"I. Kulich <i>et al.</i>, “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” <i>Science</i>, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300.","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>.","apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. 2026;392(6795):296-300. doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>","mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>.","ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300."},"quality_controlled":"1","publisher":"AAAS","status":"public","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"ddc":["580"],"date_created":"2026-04-26T22:01:47Z","day":"16","corr_author":"1","oa_version":"Accepted Version","language":[{"iso":"eng"}],"scopus_import":"1","_id":"21763","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1126/science.adu8197","volume":392,"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"oa":1,"publication_status":"published","year":"2026","abstract":[{"text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil.","lang":"eng"}],"related_material":{"record":[{"id":"23145","status":"public","relation":"dissertation_contains"}]},"title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","intvolume":"       392","has_accepted_license":"1","date_updated":"2026-10-22T09:15:44Z","month":"04","file":[{"file_size":6150733,"date_created":"2026-05-07T05:54:43Z","content_type":"application/pdf","checksum":"eb5b29247832ecdc53c8146da0509bbe","creator":"dernst","relation":"main_file","success":1,"date_updated":"2026-05-07T05:54:43Z","file_name":"2026_Science_Kulich_accepted.pdf","file_id":"21832","access_level":"open_access"}],"publication":"Science","issue":"6795","acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","pmid":1,"type":"journal_article","OA_type":"green","article_processing_charge":"No"},{"article_processing_charge":"No","type":"dissertation","supervisor":[{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří"}],"acknowledgement":"European Research Council (101142681 CYNIPS), Cyclic nucleotides as second messengers \r\nin plants, DOI: https://doi.org/10.3030/101142681 \r\nAustrian Science Fund (P 37051-B), Guanylate cyclase activity of TIR1/AFBs auxin receptors, \r\nDOI: https://doi.org/10.55776/P37051 ","month":"10","file":[{"creator":"mranduch","relation":"source_file","file_size":89544771,"date_created":"2026-10-20T09:17:28Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"4ace20ba4f1bb0f8dcc3f8eb45eb68fa","file_id":"23176","access_level":"closed","date_updated":"2026-10-20T11:48:37Z","file_name":"2026_Marek_Randuch_Thesis.docx"},{"date_updated":"2026-10-20T11:48:37Z","file_name":"2026_Marek_Randuch_Thesis.pdf","file_id":"23177","access_level":"closed","checksum":"fc630c7e8d795e7f13000cbb428424b3","content_type":"application/pdf","embargo":"2027-10-20","file_size":28321059,"date_created":"2026-10-20T09:17:20Z","embargo_to":"open_access","relation":"main_file","creator":"mranduch"}],"alternative_title":["ISTA Thesis"],"date_updated":"2026-10-22T09:15:44Z","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"12291"},{"relation":"part_of_dissertation","status":"public","id":"21763"},{"id":"23175","relation":"part_of_dissertation","status":"public"},{"id":"23174","status":"public","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"20656"}]},"has_accepted_license":"1","title":"Fast growth responses in plants: Growth regulation and phospho-proteomic ","year":"2026","license":"https://creativecommons.org/licenses/by-sa/4.0/","project":[{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"},{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681"}],"tmp":{"short":"CC BY-SA (4.0)","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","image":"/images/cc_by_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"publication_status":"published","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","fulldoi":"https://doi.org/10.15479/AT-ISTA-23145","oa_version":"Published Version","corr_author":"1","language":[{"iso":"eng"}],"_id":"23145","ddc":["580"],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"CampIT"},{"_id":"Bio"}],"doi_confirm":"1","day":"18","date_created":"2026-10-16T17:14:40Z","publisher":"Institute of Science and Technology Austria","status":"public","author":[{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek","full_name":"Randuch, Marek"}],"OA_place":"publisher","page":"289","citation":{"ama":"Randuch M. Fast growth responses in plants: Growth regulation and phospho-proteomic . 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-23145\">10.15479/AT-ISTA-23145</a>","mla":"Randuch, Marek. <i>Fast Growth Responses in Plants: Growth Regulation and Phospho-Proteomic </i>. Institute of Science and Technology Austria, 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-23145\">10.15479/AT-ISTA-23145</a>.","ista":"Randuch M. 2026. Fast growth responses in plants: Growth regulation and phospho-proteomic . Institute of Science and Technology Austria.","ieee":"M. Randuch, “Fast growth responses in plants: Growth regulation and phospho-proteomic ,” Institute of Science and Technology Austria, 2026.","short":"M. Randuch, Fast Growth Responses in Plants: Growth Regulation and Phospho-Proteomic , Institute of Science and Technology Austria, 2026.","chicago":"Randuch, Marek. “Fast Growth Responses in Plants: Growth Regulation and Phospho-Proteomic .” Institute of Science and Technology Austria, 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-23145\">https://doi.org/10.15479/AT-ISTA-23145</a>.","apa":"Randuch, M. (2026). <i>Fast growth responses in plants: Growth regulation and phospho-proteomic </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-23145\">https://doi.org/10.15479/AT-ISTA-23145</a>"},"degree_awarded":"PhD","file_date_updated":"2026-10-20T11:48:37Z","doi":"10.15479/AT-ISTA-23145","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"date_published":"2026-10-18T00:00:00Z","publication_identifier":{"issn":["2663-337X"]}},{"acknowledgement":"We would like to thank Dirk Becker (University of Wuerzburg) for his help in making the optogenetic constructs and for his comments on the manuscript. We also appreciate Rob Roelfsema (University of Wuerzburg) for his feedback on the manuscript. We sincerely thank the anonymous reviewers for the thoughtful and inspiring suggestions that helped broaden the discussion and identify important future research directions. Funding\r\nNational Key R&D Program of China, 2025YFA0923400 (RH)\r\n\r\nNational Natural Science Foundation of China (NSFC), W2531024 (RH); 32271501(NL)\r\n\r\nNSFC-Excellent Young Scientists Fund Program (overseas) (SH)\r\n\r\nNSFC-RFIS, ZB2025101410702 (RH)\r\n\r\nGuangdong Pearl River Talents Program, 2023ZT10N033\r\n\r\nChina Scholarship Council no. 202306510063 (HS)\r\n\r\nThe European Research Council grant 101142681 CYNIPS (JF)\r\n\r\nAustrian Science Fund grant FWF; P 37051-B (JF)\r\n\r\nGerman Research Foundation grant CRC 1644 (512328399; 512328399) (KK, ZH)\r\n\r\nERC Synergy project STARMORPH, reg. no. 101166880 (AP, ON)","article_processing_charge":"No","OA_type":"green","type":"preprint","title":"Ca2+ signature gates early auxin signaling in Arabidopsis roots","related_material":{"record":[{"id":"23145","relation":"dissertation_contains","status":"public"}]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.10.04.680446"}],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","abstract":[{"text":"Plants must continually balance growth with arrest, especially under stress. Auxin signaling acts as a central regulatory hub in this process, yet the mechanisms that dynamically tune early auxin signalling in real time remain unknown. Here, we used the light-gated, Ca2+-permeable Channelrhodopsin 2 variant XXM2.0 to optogenetically impose defined Ca2+ signatures on Arabidopsis root cells. Repetitive light activation triggered cytosolic Ca2+ signals that in turn suppressed auxin-induced membrane depolarization and Ca2+ transients. Moreover, prolonged optogenetic Ca2+ stimulation affects auxin-responsive transcriptional reprogramming. As phenotypic output, reversible inhibition of root growth by suppressing cell division and elongation was observed. We further identify a candidate CaM7–CNGC14 module that likely mediates Ca2+-dependent gating of early auxin signalling. Our study thus introduces a synthetic biology approach to decompose calcium–auxin crosstalk in plant cells, and demonstrates that optogenetically imposed cytosolic Ca2+ signals act as dynamic regulators of auxin susceptibility in roots.","lang":"eng"}],"year":"2026","publication":"bioRxiv","date_updated":"2026-10-22T09:15:44Z","month":"08","_id":"23175","corr_author":"1","oa_version":"Preprint","language":[{"iso":"eng"}],"day":"26","date_created":"2026-10-19T11:51:47Z","oa":1,"publication_status":"draft","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051"}],"fulldoi":"https://doi.org/10.1101/2025.10.04.680446","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JiFr"}],"biorxivid":1,"doi":"10.1101/2025.10.04.680446","external_id":{"biorxivid":["10.1101/2025.10.04.680446"]},"date_published":"2026-08-26T00:00:00Z","status":"public","citation":{"ista":"Song H, Baudon A, Freund M, Randuch M, Pěnčík A, Ondřej N, He Z, Kaufmann K, Li N, Gilliham M, Friml J, Hedrich R, Huang S. Ca2+ signature gates early auxin signaling in Arabidopsis roots. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.04.680446\">10.1101/2025.10.04.680446</a>.","mla":"Song, Huifang, et al. “Ca2+ Signature Gates Early Auxin Signaling in Arabidopsis Roots.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.04.680446\">10.1101/2025.10.04.680446</a>.","ama":"Song H, Baudon A, Freund M, et al. Ca2+ signature gates early auxin signaling in Arabidopsis roots. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.04.680446\">10.1101/2025.10.04.680446</a>","apa":"Song, H., Baudon, A., Freund, M., Randuch, M., Pěnčík, A., Ondřej, N., … Huang, S. (n.d.). Ca2+ signature gates early auxin signaling in Arabidopsis roots. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.04.680446\">https://doi.org/10.1101/2025.10.04.680446</a>","chicago":"Song, Huifang, Angel Baudon, Matthias Freund, Marek Randuch, Aleš Pěnčík, Novák Ondřej, Zhaohui He, et al. “Ca2+ Signature Gates Early Auxin Signaling in Arabidopsis Roots.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.04.680446\">https://doi.org/10.1101/2025.10.04.680446</a>.","short":"H. Song, A. Baudon, M. Freund, M. Randuch, A. Pěnčík, N. Ondřej, Z. He, K. Kaufmann, N. Li, M. Gilliham, J. Friml, R. Hedrich, S. Huang, BioRxiv (n.d.).","ieee":"H. Song <i>et al.</i>, “Ca2+ signature gates early auxin signaling in Arabidopsis roots,” <i>bioRxiv</i>. ."},"author":[{"first_name":"Huifang","full_name":"Song, Huifang","last_name":"Song"},{"first_name":"Angel","full_name":"Baudon, Angel","last_name":"Baudon"},{"last_name":"Freund","full_name":"Freund, Matthias","first_name":"Matthias"},{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek","full_name":"Randuch, Marek"},{"first_name":"Aleš","full_name":"Pěnčík, Aleš","last_name":"Pěnčík"},{"full_name":"Ondřej, Novák","first_name":"Novák","last_name":"Ondřej"},{"last_name":"He","full_name":"He, Zhaohui","first_name":"Zhaohui"},{"first_name":"Kerstin","full_name":"Kaufmann, Kerstin","last_name":"Kaufmann"},{"first_name":"Nan","full_name":"Li, Nan","last_name":"Li"},{"first_name":"Matthew","full_name":"Gilliham, Matthew","last_name":"Gilliham"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"},{"last_name":"Hedrich","first_name":"Rainer","full_name":"Hedrich, Rainer"},{"first_name":"Shouguang","full_name":"Huang, Shouguang","last_name":"Huang"}],"OA_place":"repository"},{"issue":"4","acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","article_number":"koaf006","pmid":1,"type":"journal_article","OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","year":"2025","abstract":[{"text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. ","lang":"eng"}],"related_material":{"record":[{"id":"18837","status":"public","relation":"research_data"},{"status":"public","relation":"earlier_version","id":"18689"}]},"title":"Super-resolution expansion microscopy in plant roots","intvolume":"        37","has_accepted_license":"1","date_updated":"2026-10-20T09:19:12Z","file":[{"content_type":"application/pdf","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","date_created":"2025-07-31T07:03:43Z","file_size":53904111,"relation":"main_file","success":1,"creator":"dernst","file_name":"2025_PlantCell_Gallei.pdf","date_updated":"2025-07-31T07:03:43Z","access_level":"open_access","file_id":"20092"}],"month":"04","publication":"The Plant Cell","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"ec_funded":1,"ddc":["580"],"day":"01","date_created":"2025-02-05T06:52:06Z","corr_author":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","scopus_import":"1","_id":"19003","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1093/plcell/koaf006","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":37,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742985"},{"call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"},{"grant_number":"754411","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF 679-2018","_id":"269B5B22-B435-11E9-9278-68D0E5697425","name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","grant_number":"26137"},{"grant_number":"W1232-B24","name":"Molecular Drug Targets","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"publication_status":"published","oa":1,"publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"external_id":{"isi":["001462763100001"],"pmid":["39792900"]},"date_published":"2025-04-01T00:00:00Z","article_type":"original","PlanS_conform":"1","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"doi":"10.1093/plcell/koaf006","file_date_updated":"2025-07-31T07:03:43Z","author":[{"orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","first_name":"Michelle C"},{"last_name":"Truckenbrodt","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M","first_name":"Sven M"},{"full_name":"Kreuzinger, Caroline","first_name":"Caroline","last_name":"Kreuzinger","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Syamala","full_name":"Inumella, Syamala","orcid":"0009-0002-5890-120X","id":"F8660870-D756-11E9-98C5-34DFE5697425","last_name":"Inumella"},{"full_name":"Vistunou, Vitali","first_name":"Vitali","last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81"},{"first_name":"Christoph M","full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer"},{"orcid":"0000-0002-7667-6854","last_name":"Tavakoli","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","full_name":"Tavakoli, Mojtaba","first_name":"Mojtaba"},{"last_name":"Agudelo Duenas","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","full_name":"Agudelo Duenas, Nathalie","first_name":"Nathalie"},{"full_name":"Vorlaufer, Jakob","first_name":"Jakob","last_name":"Vorlaufer","id":"937696FA-C996-11E9-8C7C-CF13E6697425","orcid":"0009-0000-7590-3501"},{"orcid":"0000-0003-0201-2315","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","last_name":"Jahr","first_name":"Wiebke","full_name":"Jahr, Wiebke"},{"full_name":"Randuch, Marek","first_name":"Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Alexander J","full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson"},{"full_name":"Benková, Eva","first_name":"Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","orcid":"0000-0001-8559-3973","first_name":"Johann G","full_name":"Danzl, Johann G"}],"OA_place":"publisher","isi":1,"citation":{"apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>The Plant Cell</i>, vol. 37, no. 4. Oxford University Press, 2025.","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. 2025;37(4). doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>"},"quality_controlled":"1","publisher":"Oxford University Press","status":"public"},{"_id":"20656","oa_version":"Published Version","language":[{"iso":"eng"}],"corr_author":"1","date_created":"2025-11-19T09:44:31Z","day":"30","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"ec_funded":1,"ddc":["580"],"oa":1,"publication_status":"published","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"grant_number":"ALTF 985-2016","_id":"26060676-B435-11E9-9278-68D0E5697425","name":"Cell surface receptor complexes for auxin signaling in plants"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"}],"volume":188,"fulldoi":"https://doi.org/10.1016/j.cell.2025.08.026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JiFr"},{"_id":"XiFe"}],"file_date_updated":"2025-11-24T10:55:18Z","doi":"10.1016/j.cell.2025.08.026","article_type":"original","PlanS_conform":"1","publication_identifier":{"issn":["0092-8674"]},"external_id":{"isi":["001616077900005"],"pmid":["41043433"]},"date_published":"2025-10-30T00:00:00Z","status":"public","publisher":"Cell Press","citation":{"ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. <i>Cell</i>. 2025;188(22):6138-6150.e17. doi:<a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">10.1016/j.cell.2025.08.026</a>","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Kulich I, Jaillais Y, Friml J. 2025. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell. 188(22), 6138–6150.e17.","mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>Cell</i>, vol. 188, no. 22, Cell Press, 2025, p. 6138–6150.e17, doi:<a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">10.1016/j.cell.2025.08.026</a>.","short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, I. Kulich, Y. Jaillais, J. Friml, Cell 188 (2025) 6138–6150.e17.","ieee":"L. Rodriguez Solovey <i>et al.</i>, “ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism,” <i>Cell</i>, vol. 188, no. 22. Cell Press, p. 6138–6150.e17, 2025.","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (2025). ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">https://doi.org/10.1016/j.cell.2025.08.026</a>","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>Cell</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.cell.2025.08.026\">https://doi.org/10.1016/j.cell.2025.08.026</a>."},"isi":1,"quality_controlled":"1","page":"6138-6150.e17","author":[{"first_name":"Lesia","full_name":"Rodriguez Solovey, Lesia","orcid":"0000-0002-7244-7237","id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey"},{"last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986","full_name":"Fiedler, Lukas","first_name":"Lukas"},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","last_name":"Zou","first_name":"Minxia","full_name":"Zou, Minxia"},{"last_name":"Giannini","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","full_name":"Giannini, Caterina","first_name":"Caterina"},{"last_name":"Monzer","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline","first_name":"Aline"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek","full_name":"Randuch, Marek"},{"first_name":"Yongfan","full_name":"Yu, Yongfan","last_name":"Yu"},{"orcid":"0000-0003-4783-1752","last_name":"Gelová","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","full_name":"Gelová, Zuzana","first_name":"Zuzana"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten","orcid":"0000-0001-7241-2328","first_name":"Inge","full_name":"Verstraeten, Inge"},{"last_name":"Hajny","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub","first_name":"Jakub"},{"full_name":"Chen, Meng","first_name":"Meng","last_name":"Chen"},{"orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","first_name":"Shutang","full_name":"Tan, Shutang"},{"orcid":"0000-0001-8295-2926","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Hörmayer","first_name":"Lukas","full_name":"Hörmayer, Lukas"},{"full_name":"Li, Lanxin","first_name":"Lanxin","last_name":"Li","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5607-272X"},{"last_name":"Marques-Bueno","first_name":"Maria Mar","full_name":"Marques-Bueno, Maria Mar"},{"first_name":"Zainab","full_name":"Quddoos, Zainab","id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466","last_name":"Quddoos"},{"full_name":"Molnar, Gergely","first_name":"Gergely","last_name":"Molnar","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87"},{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","first_name":"Ivan","full_name":"Kulich, Ivan"},{"first_name":"Yvon","full_name":"Jaillais, Yvon","last_name":"Jaillais"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"OA_place":"publisher","acknowledgement":"We gratefully acknowledge Tongda Xu for experimental, material, and conceptual support. We thank William Gray for providing material, Nataliia Gnyliukh and Ema Cervenova for help with manuscript preparation, and Julia Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at the Institute of Science and Technology Austria (ISTA) for their excellent service and assistance. The research leading to these results has received funding from the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; I 6123-B) to J.F., and Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by the FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985-2016). S.T. was supported by the National Natural Science Foundation of China (32321001, 32570366). The work of J.H. was supported by the project JG_2024_003 implemented within the Palacký University Young Researcher Grant.","issue":"22","article_processing_charge":"Yes (via OA deal)","OA_type":"hybrid","type":"journal_article","pmid":1,"title":"ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism","intvolume":"       188","has_accepted_license":"1","related_material":{"record":[{"id":"19399","relation":"earlier_version","status":"public"},{"status":"public","relation":"dissertation_contains","id":"23145"}]},"abstract":[{"text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a prerequisite for (1) self-organizing processes, including vascular tissue formation, and (2) directional growth responses such as gravitropism. Here, we identify a mechanism by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1) receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably for other self-organizing developmental phenomena.","lang":"eng"}],"year":"2025","publication":"Cell","date_updated":"2026-10-22T09:15:44Z","month":"10","file":[{"creator":"dernst","relation":"main_file","success":1,"file_size":17825465,"date_created":"2025-11-24T10:55:18Z","content_type":"application/pdf","checksum":"8ac396a0806ad7f2e4e7a0c1eed712ce","file_id":"20679","access_level":"open_access","date_updated":"2025-11-24T10:55:18Z","file_name":"2025_Cell_Rodriguez.pdf"}]},{"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"publication_status":"draft","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","fulldoi":"https://doi.org/10.1101/2025.10.17.683082","language":[{"iso":"eng"}],"corr_author":"1","oa_version":"Preprint","_id":"23174","day":"17","date_created":"2026-10-19T11:44:48Z","status":"public","author":[{"full_name":"Randuch, Marek","first_name":"Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"full_name":"Kulich, Ivan","first_name":"Ivan","last_name":"Kulich","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii","first_name":"Dmitrii"},{"full_name":"Huang, Shouguang","first_name":"Shouguang","last_name":"Huang"},{"full_name":"Hedrich, Rainer","first_name":"Rainer","last_name":"Hedrich"},{"first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"OA_place":"repository","citation":{"ama":"Randuch M, Kulich I, Vladimirtsev D, Huang S, Hedrich R, Friml J. Cytosolic Ca2+ as a universal signal for rapid root growth regulation. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.17.683082\">10.1101/2025.10.17.683082</a>","mla":"Randuch, Marek, et al. “Cytosolic Ca2+ as a Universal Signal for Rapid Root Growth Regulation.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.17.683082\">10.1101/2025.10.17.683082</a>.","ista":"Randuch M, Kulich I, Vladimirtsev D, Huang S, Hedrich R, Friml J. Cytosolic Ca2+ as a universal signal for rapid root growth regulation. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.17.683082\">10.1101/2025.10.17.683082</a>.","ieee":"M. Randuch, I. Kulich, D. Vladimirtsev, S. Huang, R. Hedrich, and J. Friml, “Cytosolic Ca2+ as a universal signal for rapid root growth regulation,” <i>bioRxiv</i>. .","short":"M. Randuch, I. Kulich, D. Vladimirtsev, S. Huang, R. Hedrich, J. Friml, BioRxiv (n.d.).","chicago":"Randuch, Marek, Ivan Kulich, Dmitrii Vladimirtsev, Shouguang Huang, Rainer Hedrich, and Jiří Friml. “Cytosolic Ca2+ as a Universal Signal for Rapid Root Growth Regulation.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.17.683082\">https://doi.org/10.1101/2025.10.17.683082</a>.","apa":"Randuch, M., Kulich, I., Vladimirtsev, D., Huang, S., Hedrich, R., &#38; Friml, J. (n.d.). Cytosolic Ca2+ as a universal signal for rapid root growth regulation. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.17.683082\">https://doi.org/10.1101/2025.10.17.683082</a>"},"doi":"10.1101/2025.10.17.683082","biorxivid":1,"department":[{"_id":"JiFr"}],"date_published":"2025-10-17T00:00:00Z","external_id":{"biorxivid":["10.1101/2025.10.17.683082"]},"OA_type":"green","article_processing_charge":"No","type":"preprint","acknowledgement":"We gratefully acknowledge Dr. Andrea Bleckmann, creator of pBLAX000 vector and Dr. Shiqiang Gao for donation of template used in XXM-ER cloning. This research was supported by the Laboratory Support Facility, the Plant Facility, the IT Facility and the Imaging and Optical Facility of the Institute of Science and Technology Austria. The graphical elements used in Fig. 5 were adapted and modified from Servier, https://smart.servier.com, and are used under CC BY 3.0 licence. The authors acknowledge the use of AI-tools (Microsoft Copilot, Gemini) for language refinement. All content was critically reviewed by authors.\r\n\r\nFunder Information Declared\r\nEuropean Research Council, 101142681 CYNIPS\r\nAustrian Science Fund, P 37051-B","month":"10","date_updated":"2026-10-22T09:15:44Z","publication":"bioRxiv","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"23145"}]},"title":"Cytosolic Ca2+ as a universal signal for rapid root growth regulation","year":"2025","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.10.17.683082"}],"abstract":[{"text":"Roots must continuously adapt their growth and have evolved the ability to rapidly respond to diverse environmental and hormonal cues, including the phytohormone auxin. Auxin-induced cellular responses involve membrane depolarization, cytosolic calcium (Ca²⁺) elevation, and extracellular pH increase, but how these processes lead to rapid growth regulation remains unclear. Here, we show that cytosolic Ca²⁺ acts as a universal signal integrating diverse cues for rapid regulation of root growth. Using live imaging, microfluidics, and optogenetics in Arabidopsis, we demonstrate that a swift rise in cytosolic Ca²⁺ is the primary target of auxin signaling affecting growth inhibition. Disruption of Ca²⁺ influx abolishes these responses, whereas light-gated Ca²⁺ influx from the apoplast or endoplasmic reticulum stores inhibits growth. Multiple unrelated stimuli—including auxin, extracellular ATP, RALF peptides, and hydrogen peroxide—converge on this Ca²⁺-dependent mechanism. Cytosolic Ca²⁺ elevation thus represents a necessary and sufficient step for rapid growth inhibition, revealing a unifying principle of root signaling.","lang":"eng"}]},{"acknowledgement":"We thank W. Gray for providing material; N. Gnyliukh and E. Cervenova for help with manuscript preparation; J. Schmid for help with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science facilities at ISTA for their excellent service and assistance. The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program grant agreement No 742985 and Austrian Science Fund (FWF): I3630-775 B25 to J.F; National Natural Science Foundation of China (Grant 32130010, 31422008), start-up funds from FAFU to T.X., Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013. L.R. was supported by FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 985- 2016). S.T. was supported by the National Natural Science Foundation of China (32321001).","das_tickbox":"1","type":"preprint","article_processing_charge":"No","OA_type":"green","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2022.11.30.518503"}],"abstract":[{"text":"Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a key prerequisite for (i) self-organizing processes including vascular tissue formation and (ii) directional growth responses such as gravitropism. Here we identify a mechanism, by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface ABP1-TMK1 receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE auxin receptor ABL3. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably also for other self-organizing developmental phenomena.","lang":"eng"}],"year":"2025","title":"ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism","related_material":{"record":[{"id":"19395","relation":"dissertation_contains","status":"public"},{"id":"20656","status":"public","relation":"later_version"},{"id":"20364","status":"public","relation":"dissertation_contains"}]},"publication":"bioRxiv","month":"02","date_updated":"2026-10-27T23:31:08Z","date_created":"2025-03-13T08:36:48Z","day":"20","ec_funded":1,"ddc":["580"],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"_id":"19399","corr_author":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","fulldoi":"https://doi.org/10.1101/2022.11.30.518503","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"draft","oa":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742985"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I03630"},{"_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734"},{"name":"Cell surface receptor complexes for auxin signaling in plants","_id":"26060676-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 985-2016"}],"date_published":"2025-02-20T00:00:00Z","doi":"10.1101/2022.11.30.518503","department":[{"_id":"JiFr"},{"_id":"XiFe"}],"citation":{"mla":"Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2022.11.30.518503\">10.1101/2022.11.30.518503</a>.","ista":"Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Xu T, Kulich I, Jaillais Y, Friml J. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. bioRxiv, <a href=\"https://doi.org/10.1101/2022.11.30.518503\">10.1101/2022.11.30.518503</a>.","ama":"Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2022.11.30.518503\">10.1101/2022.11.30.518503</a>","chicago":"Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini, Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2022.11.30.518503\">https://doi.org/10.1101/2022.11.30.518503</a>.","apa":"Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev, D., … Friml, J. (n.d.). ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2022.11.30.518503\">https://doi.org/10.1101/2022.11.30.518503</a>","ieee":"L. Rodriguez Solovey <i>et al.</i>, “ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated auxin fluxes for root gravitropism,” <i>bioRxiv</i>. .","short":"L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev, M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer, L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, T. Xu, I. Kulich, Y. Jaillais, J. Friml, BioRxiv (n.d.)."},"author":[{"full_name":"Rodriguez Solovey, Lesia","first_name":"Lesia","orcid":"0000-0002-7244-7237","last_name":"Rodriguez Solovey","id":"3922B506-F248-11E8-B48F-1D18A9856A87"},{"id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler","first_name":"Lukas","full_name":"Fiedler, Lukas"},{"last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","first_name":"Minxia"},{"first_name":"Caterina","full_name":"Giannini, Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","last_name":"Giannini"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","first_name":"Aline","full_name":"Monzer, Aline"},{"id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev","first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii"},{"last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek","first_name":"Marek"},{"last_name":"Yu","full_name":"Yu, Yongfan","first_name":"Yongfan"},{"orcid":"0000-0003-4783-1752","last_name":"Gelová","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","full_name":"Gelová, Zuzana","first_name":"Zuzana"},{"last_name":"Verstraeten","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","first_name":"Inge"},{"full_name":"Hajny, Jakub","first_name":"Jakub","last_name":"Hajny","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195"},{"last_name":"Chen","full_name":"Chen, Meng","first_name":"Meng"},{"orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","first_name":"Shutang","full_name":"Tan, Shutang"},{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Hörmayer","orcid":"0000-0001-8295-2926","first_name":"Lukas","full_name":"Hörmayer, Lukas"},{"last_name":"Li","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5607-272X","full_name":"Li, Lanxin","first_name":"Lanxin"},{"last_name":"Marques-Bueno","full_name":"Marques-Bueno, Maria Mar","first_name":"Maria Mar"},{"first_name":"Zainab","full_name":"Quddoos, Zainab","id":"32ff3c64-04a0-11f0-a50f-d0c45bfac466","last_name":"Quddoos"},{"full_name":"Molnar, Gergely","first_name":"Gergely","last_name":"Molnar","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Tongda","full_name":"Xu, Tongda","last_name":"Xu"},{"first_name":"Ivan","full_name":"Kulich, Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich"},{"last_name":"Jaillais","full_name":"Jaillais, Yvon","first_name":"Yvon"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"OA_place":"repository","status":"public"},{"title":"Super-resolution expansion microscopy in plant roots","related_material":{"record":[{"id":"19003","relation":"later_version","status":"public"},{"id":"18681","relation":"dissertation_contains","status":"public"}]},"license":"https://creativecommons.org/licenses/by-nc/4.0/","abstract":[{"text":"Multiplexed fluorescence microscopy imaging is widely used in biomedical applications. However, simultaneous imaging of multiple fluorophores can result in spectral leaks and overlapping, which greatly degrades image quality and subsequent analysis. Existing popular spectral unmixing methods are mainly based on computational intensive linear models and the performance is heavily dependent on the reference spectra, which may greatly preclude its further applications. In this paper, we propose a deep learning-based blindly spectral unmixing method, termed AutoUnmix, to imitate the physical spectral mixing process. A tranfer learning framework is further devised to allow our AutoUnmix adapting to a variety of imaging systems without retraining the network. Our proposed method has demonstrated real-time unmixing capabilities, surpassing existing methods by up to 100-fold in terms of unmixing speed. We further validate the reconstruction performance on both synthetic datasets and biological samples. The unmixing results of AutoUnmix achieve a highest SSIM of 0.99 in both three- and four-color imaging, with nearly up to 20% higher than other popular unmixing methods. Due to the desirable property of data independency and superior blind unmixing performance, we believe AutoUnmix is a powerful tool to study the interaction process of different organelles labeled by multiple fluorophores.","lang":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.02.21.581330"}],"year":"2024","publication":"bioRxiv","month":"02","researchdata_availability":"no","date_updated":"2026-10-02T11:27:43Z","supplementarymaterial":"no","das_tickbox":"0","acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\n\r\nThis project has received funding from the Austrian Science Fund (FWF): I 3630-B25 (J.G.D) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 742985, J.F.). It has also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385. S.T. has received funding as an ISTplus Fellow from the European Union’s Horizon 2020 Research and Innovation Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from an EMBO Long-Term Fellowship (grant number ALTF 679-2018). It has further received funding from the Austrian Science Fund (FWF) grant DK W1232 (M.T, N.A-D., J.G.D). W.J. received funding via a Human Frontier Science Program postdoctoral fellowship LT000557/2018.\r\n\r\nThe funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","article_processing_charge":"No","type":"preprint","doi":"10.1101/2024.02.21.581330","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"date_published":"2024-02-21T00:00:00Z","status":"public","citation":{"short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, BioRxiv (n.d.).","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>bioRxiv</i>. .","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (n.d.). Super-resolution expansion microscopy in plant roots. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.02.21.581330\">https://doi.org/10.1101/2024.02.21.581330</a>","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.02.21.581330\">https://doi.org/10.1101/2024.02.21.581330</a>.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. Super-resolution expansion microscopy in plant roots. bioRxiv, <a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>.","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>."},"OA_place":"repository","author":[{"first_name":"Michelle C","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei"},{"last_name":"Truckenbrodt","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M","first_name":"Sven M"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","last_name":"Kreuzinger","first_name":"Caroline","full_name":"Kreuzinger, Caroline"},{"orcid":"0009-0002-5890-120X","last_name":"Inumella","id":"F8660870-D756-11E9-98C5-34DFE5697425","full_name":"Inumella, Syamala","first_name":"Syamala"},{"id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","last_name":"Vistunou","first_name":"Vitali","full_name":"Vistunou, Vitali"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M"},{"last_name":"Tavakoli","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba","first_name":"Mojtaba"},{"full_name":"Agudelo Duenas, Nathalie","first_name":"Nathalie","last_name":"Agudelo Duenas","id":"40E7F008-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0009-0000-7590-3501","last_name":"Vorlaufer","id":"937696FA-C996-11E9-8C7C-CF13E6697425","full_name":"Vorlaufer, Jakob","first_name":"Jakob"},{"orcid":"0000-0003-0201-2315","last_name":"Jahr","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","first_name":"Wiebke"},{"last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek","first_name":"Marek"},{"full_name":"Johnson, Alexander J","first_name":"Alexander J","last_name":"Johnson","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843"},{"orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva"},{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Danzl, Johann G","first_name":"Johann G","last_name":"Danzl","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973"}],"_id":"18689","corr_author":"1","oa_version":"Preprint","language":[{"iso":"eng"}],"day":"21","date_created":"2024-12-19T12:28:00Z","ec_funded":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"oa":1,"publication_status":"draft","tmp":{"short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"},{"grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020"},{"call_identifier":"FWF","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","grant_number":"W1232-B24"},{"_id":"269B5B22-B435-11E9-9278-68D0E5697425","name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","grant_number":"ALTF 679-2018"}],"fulldoi":"https://doi.org/10.1101/2024.02.21.581330","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"pmid":1,"type":"journal_article","article_processing_charge":"Yes","issue":"5","acknowledgement":"The authors wish to acknowledge Dr. Daniel van Damme for mRuby3/pDONRP2rP3 and Prof. Qi-Jun Chen for sharing plasmids used for CRISPR-Cas9 mutagenesis. This work was supported by the Austrian Science Fund (FWF): I 3630-B25.","das_tickbox":"1","supplementarymaterial":"yes","article_number":"114195","date_updated":"2026-10-20T09:19:12Z","researchdata_availability":"upon request","file":[{"file_id":"15387","access_level":"open_access","date_updated":"2024-05-13T12:11:22Z","file_name":"2024_CellReports_Adamowski.pdf","relation":"main_file","success":1,"creator":"dernst","checksum":"a06bb85be4fc765c51554d27ee2da802","content_type":"application/pdf","file_size":5698598,"date_created":"2024-05-13T12:11:22Z"}],"month":"05","publication":"Cell Reports","year":"2024","abstract":[{"text":"Clathrin-mediated endocytosis (CME) is an essential process of cargo uptake operating in all eukaryotes. In animals and yeast, BAR-SH3 domain proteins, endophilins and amphiphysins, function at the conclusion of CME to recruit factors for vesicle scission and uncoating. Arabidopsis thaliana contains the BAR-SH3 domain proteins SH3P1–SH3P3, but their role is poorly understood. Here, we identify SH3Ps as functional homologs of endophilin/amphiphysin. SH3P1–SH3P3 bind to discrete foci at the plasma membrane (PM), and SH3P2 recruits late to a subset of clathrin-coated pits. The SH3P2 PM recruitment pattern is nearly identical to its interactor, a putative uncoating factor, AUXILIN-LIKE1. Notably, SH3P1–SH3P3 are required for most of AUXILIN-LIKE1 recruitment to the PM. This indicates a plant-specific modification of CME, where BAR-SH3 proteins recruit auxilin-like uncoating factors rather than the uncoating phosphatases, synaptojanins. SH3P1–SH3P3 act redundantly in overall CME with the plant-specific endocytic adaptor TPLATE complex but not due to an SH3 domain in its TASH3 subunit.","lang":"eng"}],"intvolume":"        43","title":"SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis","has_accepted_license":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","fulldoi":"https://doi.org/10.1016/j.celrep.2024.114195","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"dataavailabilitystatement":"All data reported in this paper will be shared by the lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this work paper is available from the lead contact upon request.","volume":43,"project":[{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"publication_status":"published","oa":1,"ddc":["580"],"date_created":"2024-05-12T22:01:01Z","day":"28","corr_author":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","scopus_import":"1","_id":"15374","author":[{"first_name":"Maciek","full_name":"Adamowski, Maciek","orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","last_name":"Adamowski"},{"id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","last_name":"Randuch","first_name":"Marek","full_name":"Randuch, Marek"},{"first_name":"Ivana","full_name":"Matijevic, Ivana","id":"83c17ce3-15b2-11ec-abd3-f486545870bd","last_name":"Matijevic"},{"full_name":"Narasimhan, Madhumitha","first_name":"Madhumitha","orcid":"0000-0002-8600-0671","last_name":"Narasimhan","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří"}],"isi":1,"citation":{"ieee":"M. Adamowski, M. Randuch, I. Matijevic, M. Narasimhan, and J. Friml, “SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis,” <i>Cell Reports</i>, vol. 43, no. 5. Cell Press, 2024.","short":"M. Adamowski, M. Randuch, I. Matijevic, M. Narasimhan, J. Friml, Cell Reports 43 (2024).","chicago":"Adamowski, Maciek, Marek Randuch, Ivana Matijevic, Madhumitha Narasimhan, and Jiří Friml. “SH3Ps Recruit Auxilin-like Vesicle Uncoating Factors for Clathrin-Mediated Endocytosis.” <i>Cell Reports</i>. Cell Press, 2024. <a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">https://doi.org/10.1016/j.celrep.2024.114195</a>.","apa":"Adamowski, M., Randuch, M., Matijevic, I., Narasimhan, M., &#38; Friml, J. (2024). SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">https://doi.org/10.1016/j.celrep.2024.114195</a>","ama":"Adamowski M, Randuch M, Matijevic I, Narasimhan M, Friml J. SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. <i>Cell Reports</i>. 2024;43(5). doi:<a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">10.1016/j.celrep.2024.114195</a>","mla":"Adamowski, Maciek, et al. “SH3Ps Recruit Auxilin-like Vesicle Uncoating Factors for Clathrin-Mediated Endocytosis.” <i>Cell Reports</i>, vol. 43, no. 5, 114195, Cell Press, 2024, doi:<a href=\"https://doi.org/10.1016/j.celrep.2024.114195\">10.1016/j.celrep.2024.114195</a>.","ista":"Adamowski M, Randuch M, Matijevic I, Narasimhan M, Friml J. 2024. SH3Ps recruit auxilin-like vesicle uncoating factors for clathrin-mediated endocytosis. Cell Reports. 43(5), 114195."},"quality_controlled":"1","publisher":"Cell Press","status":"public","publication_identifier":{"eissn":["2211-1247"]},"external_id":{"pmid":["38717900"],"isi":["001240362800001"]},"date_published":"2024-05-28T00:00:00Z","article_type":"original","department":[{"_id":"JiFr"},{"_id":"MaLo"}],"doi":"10.1016/j.celrep.2024.114195","file_date_updated":"2024-05-13T12:11:22Z"},{"department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"EvBe"},{"_id":"EM-Fac"}],"doi":"10.1038/s41586-022-05187-x","file_date_updated":"2023-11-02T17:12:37Z","article_type":"original","external_id":{"isi":["000851357500002"],"pmid":["36071161"]},"publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"date_published":"2022-09-15T00:00:00Z","status":"public","publisher":"Springer Nature","citation":{"ieee":"J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 575–581, 2022.","short":"J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini, P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza, K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature 609 (2022) 575–581.","chicago":"Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>.","apa":"Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A., … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>","ama":"Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>","mla":"Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 575–81, doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>.","ista":"Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581."},"isi":1,"quality_controlled":"1","page":"575-581","author":[{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří"},{"orcid":"0000-0003-1286-7368","last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","first_name":"Michelle C"},{"id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","last_name":"Gelová","orcid":"0000-0003-4783-1752","first_name":"Zuzana","full_name":"Gelová, Zuzana"},{"orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","first_name":"Alexander J","full_name":"Johnson, Alexander J"},{"last_name":"Mazur","first_name":"Ewa","full_name":"Mazur, Ewa"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","first_name":"Aline","full_name":"Monzer, Aline"},{"first_name":"Lesia","full_name":"Rodriguez Solovey, Lesia","orcid":"0000-0002-7244-7237","id":"3922B506-F248-11E8-B48F-1D18A9856A87","last_name":"Rodriguez Solovey"},{"last_name":"Roosjen","first_name":"Mark","full_name":"Roosjen, Mark"},{"full_name":"Verstraeten, Inge","first_name":"Inge","orcid":"0000-0001-7241-2328","last_name":"Verstraeten","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Branka D.","full_name":"Živanović, Branka D.","last_name":"Živanović"},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","last_name":"Zou","first_name":"Minxia","full_name":"Zou, Minxia"},{"first_name":"Lukas","full_name":"Fiedler, Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler"},{"last_name":"Giannini","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","full_name":"Giannini, Caterina","first_name":"Caterina"},{"last_name":"Grones","full_name":"Grones, Peter","first_name":"Peter"},{"full_name":"Hrtyan, Mónika","first_name":"Mónika","last_name":"Hrtyan","id":"45A71A74-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Walter","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann"},{"first_name":"Andre","full_name":"Kuhn, Andre","last_name":"Kuhn"},{"orcid":"0000-0002-8600-0671","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","last_name":"Narasimhan","first_name":"Madhumitha","full_name":"Narasimhan, Madhumitha"},{"full_name":"Randuch, Marek","first_name":"Marek","last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"last_name":"Rýdza","full_name":"Rýdza, Nikola","first_name":"Nikola"},{"last_name":"Takahashi","full_name":"Takahashi, Koji","first_name":"Koji"},{"first_name":"Shutang","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan"},{"id":"e3736151-106c-11ec-b916-c2558e2762c6","last_name":"Teplova","first_name":"Anastasiia","full_name":"Teplova, Anastasiia"},{"last_name":"Kinoshita","full_name":"Kinoshita, Toshinori","first_name":"Toshinori"},{"full_name":"Weijers, Dolf","first_name":"Dolf","last_name":"Weijers"},{"full_name":"Rakusová, Hana","first_name":"Hana","last_name":"Rakusová"}],"_id":"12291","language":[{"iso":"eng"}],"oa_version":"Submitted Version","corr_author":"1","scopus_import":"1","date_created":"2023-01-16T10:04:48Z","day":"15","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"ec_funded":1,"ddc":["580"],"publication_status":"published","oa":1,"volume":609,"project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425","name":"RNA-directed DNA methylation in plant development","grant_number":"P29988"}],"fulldoi":"https://doi.org/10.1038/s41586-022-05187-x","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"ABP1–TMK auxin perception for global phosphorylation and auxin canalization","intvolume":"       609","has_accepted_license":"1","related_material":{"record":[{"id":"19395","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"23145"},{"id":"20364","relation":"dissertation_contains","status":"public"}]},"abstract":[{"text":"The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization.","lang":"eng"}],"year":"2022","publication":"Nature","date_updated":"2026-10-27T23:31:08Z","month":"09","file":[{"access_level":"open_access","file_id":"14483","file_name":"Friml Nature 2022_merged.pdf","date_updated":"2023-11-02T17:12:37Z","creator":"amally","success":1,"relation":"main_file","date_created":"2023-11-02T17:12:37Z","file_size":79774945,"checksum":"a6055c606aefb900bf62ae3e7d15f921","content_type":"application/pdf"}],"acknowledgement":"We acknowledge K. Kubiasová for excellent technical assistance, J. Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI; and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM (MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F. is grateful to R. Napier for many insightful suggestions and support. We thank all past and present members of the Friml group for their support and for other contributions to this effort to clarify the controversial role of ABP1 over the past seven years. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.); the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001 to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053 to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910).","issue":"7927","article_processing_charge":"No","type":"journal_article","pmid":1}]