---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21711'
abstract:
- lang: eng
  text: 'Background: Low-volume trapping columns are essential for sample enrichment,
    desalting, and injection profile focusing on nano-LC–MS-based proteomics. They
    enable higher sample loading, improve chromatographic performance, and protect
    the analytical column by removing salts and contaminants. Recently, monolithic
    trap columns with micropillar architecture have emerged as alternatives to conventionally
    packed traps. This study compares the performance of a packed and a micropillar
    monolithic trap column for the analysis of tryptic peptides. Methods: A tryptic
    digest of HeLa cell lysate was analyzed under identical LC–MS conditions using
    both trap types. Peptides were detected at 214 nm and analyzed by nano-ESI on
    a Q Exactive Plus Orbitrap. Data were searched against the human UniProt database
    (February 2023) using FragPipe v20.0, and statistical evaluation of MaxLFQ intensities
    was performed in Perseus using Welch’s t-test and clustering analysis. Results:
    Over 2500 proteins were identified with both setups. The packed trap column yielded
    more total peptides, particularly those with post-translational modifications
    and higher hydrophilicity, whereas the monolithic column favored peptides of intermediate
    hydrophobicity. Chromatographic profiles confirmed a slight reduction in the trapping
    efficiency of hydrophilic peptides by the monolithic trap. Conclusions: Trap column
    design significantly influences peptide recovery and proteome coverage.'
acknowledgement: 'The authors thank Gábor Tóth, Uppsala University, Sweden, and Armel
  Nicolas, Institute for Science and Technology Austria, for their support. This research
  was conducted during a student residency in Vienna under the auspices of OeAD. ZI:
  ICM-2016-03196.'
article_number: '10'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jadranka
  full_name: Miletić Vukajlović, Jadranka
  last_name: Miletić Vukajlović
- first_name: Bojana
  full_name: Ilić, Bojana
  last_name: Ilić
- first_name: Bella
  full_name: Bruszel, Bella
  id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
  last_name: Bruszel
- first_name: Tanja
  full_name: Panić-Janković, Tanja
  last_name: Panić-Janković
- first_name: Goran
  full_name: Mitulović, Goran
  last_name: Mitulović
citation:
  ama: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. Comparison
    of the trapping efficiency for tryptic peptides on particle-packed and micro-pillar
    trap columns for proteomics analyses. <i>Proteomes</i>. 2026;14(1). doi:<a href="https://doi.org/10.3390/proteomes14010010">10.3390/proteomes14010010</a>
  apa: Miletić Vukajlović, J., Ilić, B., Bruszel, B., Panić-Janković, T., &#38; Mitulović,
    G. (2026). Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses. <i>Proteomes</i>. MDPI.
    <a href="https://doi.org/10.3390/proteomes14010010">https://doi.org/10.3390/proteomes14010010</a>
  chicago: Miletić Vukajlović, Jadranka, Bojana Ilić, Bella Bruszel, Tanja Panić-Janković,
    and Goran Mitulović. “Comparison of the Trapping Efficiency for Tryptic Peptides
    on Particle-Packed and Micro-Pillar Trap Columns for Proteomics Analyses.” <i>Proteomes</i>.
    MDPI, 2026. <a href="https://doi.org/10.3390/proteomes14010010">https://doi.org/10.3390/proteomes14010010</a>.
  ieee: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, and G. Mitulović,
    “Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses,” <i>Proteomes</i>, vol.
    14, no. 1. MDPI, 2026.
  ista: Miletić Vukajlović J, Ilić B, Bruszel B, Panić-Janković T, Mitulović G. 2026.
    Comparison of the trapping efficiency for tryptic peptides on particle-packed
    and micro-pillar trap columns for proteomics analyses. Proteomes. 14(1), 10.
  mla: Miletić Vukajlović, Jadranka, et al. “Comparison of the Trapping Efficiency
    for Tryptic Peptides on Particle-Packed and Micro-Pillar Trap Columns for Proteomics
    Analyses.” <i>Proteomes</i>, vol. 14, no. 1, 10, MDPI, 2026, doi:<a href="https://doi.org/10.3390/proteomes14010010">10.3390/proteomes14010010</a>.
  short: J. Miletić Vukajlović, B. Ilić, B. Bruszel, T. Panić-Janković, G. Mitulović,
    Proteomes 14 (2026).
date_created: 2026-04-12T22:01:49Z
date_published: 2026-03-01T00:00:00Z
date_updated: 2026-05-04T10:36:21Z
day: '01'
ddc:
- '540'
department:
- _id: MassSpec
doi: 10.3390/proteomes14010010
external_id:
  pmid:
  - '41893725'
file:
- access_level: open_access
  checksum: 1e0c66bbf4b6e0be626a8639ea664b63
  content_type: application/pdf
  creator: dernst
  date_created: 2026-05-04T10:31:35Z
  date_updated: 2026-05-04T10:31:35Z
  file_id: '21790'
  file_name: 2026_Proteomes_Vukajlovic.pdf
  file_size: 1009723
  relation: main_file
  success: 1
file_date_updated: 2026-05-04T10:31:35Z
fulldoi: https://doi.org/10.3390/proteomes14010010
has_accepted_license: '1'
intvolume: '        14'
issue: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proteomes
publication_identifier:
  eissn:
  - 2227-7382
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Comparison of the trapping efficiency for tryptic peptides on particle-packed
  and micro-pillar trap columns for proteomics analyses
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18581'
abstract:
- lang: eng
  text: "Background: Human induced pluripotent stem cells represent a scalable source
    of youthful tissue progenitors and secretomes for regenerative therapies. The
    aim of our study was to investigate the potential of conditioned medium (CM) from
    hiPSC-mesenchymal progenitors (hiPSC-MPs) to stimulate osteogenic differentiation
    of human bone marrow-derived mesenchymal stromal cells (MSCs). We also investigated
    whether prolonged cultivation or osteogenic pre-differentiation of hiPSC-MPs could
    enhance the stimulatory activity of CM.\r\nMethods: MSCs were isolated from 13
    donors (age 20–90 years). CM derived from hiPSC-MPs was added to the MSC cultures
    and the effects on proliferation and osteogenic differentiation were examined
    after 14 days and 6 weeks. The stimulatory activity of hiPSC-MP-CM was compared
    with the activity of MSC-derived CM and with the activity of CM prepared from
    hiPSC-MPs pre-cultured in growth or osteogenic medium for 14 days. Comparative
    proteomic analysis of CM was performed to gain insight into the molecular components
    responsible for the stimulatory activity.\r\nResults: Primary bone marrow-derived
    MSC exhibited variability, with a tendency towards lower proliferation and tri-lineage
    differentiation in older donors. hiPSC-MP-CM increased the proliferation and alkaline
    phosphatase activity of MSC from several adult/aged donors after 14 days of continuous
    supplementation under osteogenic conditions. However, CM supplementation failed
    to improve the mineralization of MSC pellets after 6 weeks under osteogenic conditions.
    hiPSC-MP-CM showed greater enhancement of proliferation and ALP activity than
    CM derived from bone marrow-derived MSCs. Moreover, 14-day cultivation but not
    osteogenic pre-differentiation of hiPSC-MPs strongly enhanced CM stimulatory activity.
    Quantitative proteomic analysis of d14-CM revealed a distinct profile of components
    that formed a highly interconnected associations network with two clusters, one
    functionally associated with binding and organization of actin/cytoskeletal components
    and the other with structural constituents of the extracellular matrix, collagen,
    and growth factor binding. Several hub proteins were identified that were reported
    to have functions in cell-extracellular matrix interaction, osteogenic differentiation
    and development.\r\nConclusions: Our data show that hiPSC-MP-CM enhances early
    osteogenic differentiation of human bone marrow-derived MSCs and that prolonged
    cultivation of hiPSC-MPs enhances CM-stimulatory activity. Proteomic analysis
    of the upregulated protein components provides the basis for further optimization
    of hiPSC-MP-CM for bone regenerative therapies."
acknowledgement: "We thank the personnel of the Lorenz-Böhler-Unfallkrankenhaus for
  providing the human tissue waste for primary cell isolation and the New York Stem
  Cell Foundation Research Institute for providing the human induced pluripotent stem
  cell line 1013 A and its mesenchymal progenitors. We also thank all our colleagues
  at the Ludwig Boltzmann Institute for Traumatology for their suggestions and ongoing
  support of the project. InstaText writing tool (https://instatext.io) was used to
  edit the English language of the final manuscript.\r\nThis work has received funding
  from the European Union’s Horizon 2020 research and innovation program under the
  Marie Sklodowska-Curie actions (grant agreement No. 657716) and the Transforming
  European Industry call H2020-NMBP-TRIND-2020 (grant agreement No. 953134), as well
  as by the FFG Industrienahe Dissertation program (grant agreement No. 867803 and
  853056), the FEMtech Praktika program (grant agreement No. 852154, 868917 and 877951)
  and the Production of the Future program (grant agreement No. 877452)."
article_number: '434'
article_processing_charge: Yes
article_type: original
author:
- first_name: Darja
  full_name: Marolt Presen, Darja
  last_name: Marolt Presen
- first_name: Vanessa
  full_name: Goeschl, Vanessa
  last_name: Goeschl
- first_name: Dominik
  full_name: Hanetseder, Dominik
  last_name: Hanetseder
- first_name: Laura
  full_name: Ogrin, Laura
  last_name: Ogrin
- first_name: Alexandra Larissa
  full_name: Stetco, Alexandra Larissa
  last_name: Stetco
- first_name: Anja
  full_name: Tansek, Anja
  last_name: Tansek
- first_name: Laura
  full_name: Pozenel, Laura
  last_name: Pozenel
- first_name: Bella
  full_name: Bruszel, Bella
  id: 70abbbb3-88ea-11ec-8e0a-e8c939944834
  last_name: Bruszel
- first_name: Goran
  full_name: Mitulovic, Goran
  last_name: Mitulovic
- first_name: Johannes
  full_name: Oesterreicher, Johannes
  last_name: Oesterreicher
- first_name: Johannes
  full_name: Zipperle, Johannes
  last_name: Zipperle
- first_name: Barbara
  full_name: Schaedl, Barbara
  last_name: Schaedl
- first_name: Wolfgang
  full_name: Holnthoner, Wolfgang
  last_name: Holnthoner
- first_name: Johannes
  full_name: Grillari, Johannes
  last_name: Grillari
- first_name: Heinz
  full_name: Redl, Heinz
  last_name: Redl
citation:
  ama: Marolt Presen D, Goeschl V, Hanetseder D, et al. Prolonged cultivation enhances
    the stimulatory activity of hiPSC mesenchymal progenitor-derived conditioned medium.
    <i>Stem Cell Research and Therapy</i>. 2024;15. doi:<a href="https://doi.org/10.1186/s13287-024-03960-5">10.1186/s13287-024-03960-5</a>
  apa: Marolt Presen, D., Goeschl, V., Hanetseder, D., Ogrin, L., Stetco, A. L., Tansek,
    A., … Redl, H. (2024). Prolonged cultivation enhances the stimulatory activity
    of hiPSC mesenchymal progenitor-derived conditioned medium. <i>Stem Cell Research
    and Therapy</i>. Springer Nature. <a href="https://doi.org/10.1186/s13287-024-03960-5">https://doi.org/10.1186/s13287-024-03960-5</a>
  chicago: Marolt Presen, Darja, Vanessa Goeschl, Dominik Hanetseder, Laura Ogrin,
    Alexandra Larissa Stetco, Anja Tansek, Laura Pozenel, et al. “Prolonged Cultivation
    Enhances the Stimulatory Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned
    Medium.” <i>Stem Cell Research and Therapy</i>. Springer Nature, 2024. <a href="https://doi.org/10.1186/s13287-024-03960-5">https://doi.org/10.1186/s13287-024-03960-5</a>.
  ieee: D. Marolt Presen <i>et al.</i>, “Prolonged cultivation enhances the stimulatory
    activity of hiPSC mesenchymal progenitor-derived conditioned medium,” <i>Stem
    Cell Research and Therapy</i>, vol. 15. Springer Nature, 2024.
  ista: Marolt Presen D, Goeschl V, Hanetseder D, Ogrin L, Stetco AL, Tansek A, Pozenel
    L, Bruszel B, Mitulovic G, Oesterreicher J, Zipperle J, Schaedl B, Holnthoner
    W, Grillari J, Redl H. 2024. Prolonged cultivation enhances the stimulatory activity
    of hiPSC mesenchymal progenitor-derived conditioned medium. Stem Cell Research
    and Therapy. 15, 434.
  mla: Marolt Presen, Darja, et al. “Prolonged Cultivation Enhances the Stimulatory
    Activity of HiPSC Mesenchymal Progenitor-Derived Conditioned Medium.” <i>Stem
    Cell Research and Therapy</i>, vol. 15, 434, Springer Nature, 2024, doi:<a href="https://doi.org/10.1186/s13287-024-03960-5">10.1186/s13287-024-03960-5</a>.
  short: D. Marolt Presen, V. Goeschl, D. Hanetseder, L. Ogrin, A.L. Stetco, A. Tansek,
    L. Pozenel, B. Bruszel, G. Mitulovic, J. Oesterreicher, J. Zipperle, B. Schaedl,
    W. Holnthoner, J. Grillari, H. Redl, Stem Cell Research and Therapy 15 (2024).
date_created: 2024-11-24T23:01:47Z
date_published: 2024-12-01T00:00:00Z
date_updated: 2025-09-09T11:41:12Z
day: '01'
ddc:
- '570'
department:
- _id: LifeSc
doi: 10.1186/s13287-024-03960-5
external_id:
  isi:
  - '001356479400001'
  pmid:
  - '39551765'
file:
- access_level: open_access
  checksum: 91edba8edde30d781dce89fdd5cadc39
  content_type: application/pdf
  creator: dernst
  date_created: 2024-12-10T08:28:17Z
  date_updated: 2024-12-10T08:28:17Z
  file_id: '18641'
  file_name: 2024_StemCellResearch_Presen.pdf
  file_size: 6690494
  relation: main_file
  success: 1
file_date_updated: 2024-12-10T08:28:17Z
fulldoi: https://doi.org/10.1186/s13287-024-03960-5
has_accepted_license: '1'
intvolume: '        15'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Stem Cell Research and Therapy
publication_identifier:
  eissn:
  - 1757-6512
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Prolonged cultivation enhances the stimulatory activity of hiPSC mesenchymal
  progenitor-derived conditioned medium
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2024'
...