---
OA_place: repository
OA_type: green
_id: '21994'
abstract:
- lang: eng
text: Adaptive plant development is orchestrated, among others, by directional,
intercellular transport of the phytohormone auxin. Self-organizing development,
such as flexible vasculature formation, depends on so-called auxin canalization,
manifested by the gradual formation of auxin transport channels through feedback
between auxin signalling and transport. Herein, we identify MAKR6 as an important,
novel component in this feedback. MAKR6 expression accumulates strongly in vascular
cells and is tightly regulated by auxin via the Aux/IAA-ARF-WRKY23 transcriptional
network. MAKR6 is required for auxin canalization-dependent processes, including
leaf venation, vasculature regeneration, and de novo auxin channel formation from
local auxin sources. Mechanistically, MAKR6 interacts with the PIN1 auxin transporter,
modulating its trafficking and polarization. MAKR6 also associates with and integrates
two key receptor-like kinase complexes involved in canalization, TMK1/4 and the
CAMEL-CANAR. Together, our study establishes MAKR6 as a multifaceted regulator
that couples transcriptional auxin signalling to PIN1 repolarization and coordinates
multiple RLK-mediated signalling pathways during canalization. This provides mechanistic
insights into auxin canalization and exemplifies a framework for exploring similar
regulatory nodes in other developmental contexts.
acknowledgement: 'We would like to thank Dr. Yvon Jaillais (ENS, Lyon) for sharing
MAKR2 materials. This research was supported by the Scientific Service Units (SSU)
of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the
Lab Support Facility (LSF). The research in the Friml group leading to these results
was funded by the European Research Council (ERC): 101142681 CYNIPS; and the Austrian
Science Fund (FWF): I 6123-B and P 37051-B. Ewa Mazur was supported by the National
Science Centre (NCN), Poland, under the OPUS call in the WEAVE programme: 2021/43/I/NZ1/01835.'
article_processing_charge: No
author:
- first_name: Zengxiang
full_name: Ge, Zengxiang
id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8
last_name: Ge
orcid: 0000-0001-9381-3577
- first_name: Lilla
full_name: Koczka, Lilla
last_name: Koczka
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Dmitrii
full_name: Vladimirtsev, Dmitrii
id: 60466724-5355-11ee-ae5a-fa55e8f99c3d
last_name: Vladimirtsev
- first_name: Nada
full_name: Kassem, Nada
last_name: Kassem
- first_name: Sara
full_name: Ait Ikene, Sara
id: 6a0bb896-6bad-11f1-9bef-906e9eb76034
last_name: Ait Ikene
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Ge Z, Koczka L, Mazur E, et al. MAKR6 integrates TMK and CAMEL/CANAR signalling
for auxin canalization in Arabidopsis. bioRxiv. doi:10.1101/2025.10.07.680881
apa: Ge, Z., Koczka, L., Mazur, E., Molnar, G., Vladimirtsev, D., Kassem, N., …
Friml, J. (n.d.). MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization
in Arabidopsis. bioRxiv. https://doi.org/10.1101/2025.10.07.680881
chicago: Ge, Zengxiang, Lilla Koczka, Ewa Mazur, Gergely Molnar, Dmitrii Vladimirtsev,
Nada Kassem, Sara Ait Ikene, Lukas Fiedler, and Jiří Friml. “MAKR6 Integrates
TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” BioRxiv,
n.d. https://doi.org/10.1101/2025.10.07.680881.
ieee: Z. Ge et al., “MAKR6 integrates TMK and CAMEL/CANAR signalling for
auxin canalization in Arabidopsis,” bioRxiv. .
ista: Ge Z, Koczka L, Mazur E, Molnar G, Vladimirtsev D, Kassem N, Ait Ikene S,
Fiedler L, Friml J. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin
canalization in Arabidopsis. bioRxiv, 10.1101/2025.10.07.680881.
mla: Ge, Zengxiang, et al. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for
Auxin Canalization in Arabidopsis.” BioRxiv, doi:10.1101/2025.10.07.680881.
short: Z. Ge, L. Koczka, E. Mazur, G. Molnar, D. Vladimirtsev, N. Kassem, S. Ait
Ikene, L. Fiedler, J. Friml, BioRxiv (n.d.).
corr_author: '1'
date_created: 2026-06-13T16:57:07Z
date_published: 2026-05-30T00:00:00Z
date_updated: 2026-06-19T07:14:01Z
day: '30'
ddc:
- '580'
department:
- _id: GradSch
- _id: JiFr
doi: 10.1101/2025.10.07.680881
fulldoi: https://doi.org/10.1101/2025.10.07.680881
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.10.07.680881
month: '05'
oa: 1
oa_version: Preprint
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: bioRxiv
publication_status: submitted
scopus_import: '1'
status: public
title: MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20187'
abstract:
- lang: eng
text: Very long-chain fatty acids (VLCFAs), being constituents of different types
of lipids, are critical factors in plant development, presumably due to their
impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic
reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase
1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems
(esm) mutant, a viable kcr1 allele presumably affecting surface properties of
the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that
impacts VLCFAs. The kcr1-2 plants show severe defects during different stages
of development, which all correlate with defects in polar localization and subcellular
trafficking of PIN auxin transporters and resulting asymmetric auxin distribution.
Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests
that KCR1 plays a role in delineating boundaries around meristematic and specialized
differentiating tissues, including root and shoot meristems, initiating lateral
roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced
VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful
tool to study VLCFA roles in plant development and highlights VLCFAs as critical
developmental factors at the interface of cell polarity and tissue development.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy
(especially Vanessa Zheden for technical assistance) and Life Science (in particular
Dorota Jaworska) facilities at ISTA for their continuous support. Authors would
like to thank Michelle Gallei for advice during the generation of the transgenic
lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help
and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and
root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski
for advice on abiotic stress experiments. We would like to thank Dolf Weijers for
the SOSEKI (SOK) marker line seeds. This work has benefited from the support of
IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian
Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province
(20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant
Sciences-SPS (ANR-17-EUR-0007)."
article_number: e70396
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: David
full_name: Babic, David
id: db566d23-f6e0-11ea-865d-e6f270e968e7
last_name: Babic
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Frédérique
full_name: Tellier, Frédérique
last_name: Tellier
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Hana
full_name: Rakusova, Hana
id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
last_name: Rakusova
- first_name: Petr
full_name: Valošek, Petr
id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
last_name: Valošek
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jean Denis
full_name: Faure, Jean Denis
last_name: Faure
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty
acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396
apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml,
J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis
auxin-mediated embryonic and post-embryonic development. Plant Journal.
Wiley. https://doi.org/10.1111/tpj.70396
chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier,
Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty
Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic
Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396.
ieee: D. Babic et al., “Biosynthesis of very long-chain fatty acids is required
for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant
Journal, vol. 123, no. 3. Wiley, 2025.
ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek
P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain
fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 123(3), e70396.
mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required
for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant
Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396.
short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova,
P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).
corr_author: '1'
date_created: 2025-08-17T22:01:36Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-04-07T11:52:02Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
- _id: GradSch
doi: 10.1111/tpj.70396
external_id:
isi:
- '001547884300001'
pmid:
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file:
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checksum: 1cdc3341d2d23101abca72521f1f23cb
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creator: dernst
date_created: 2025-09-01T14:09:31Z
date_updated: 2025-09-01T14:09:31Z
file_id: '20264'
file_name: 2025_PlantJournal_Babic.pdf
file_size: 5791111
relation: main_file
success: 1
file_date_updated: 2025-09-01T14:09:31Z
fulldoi: https://doi.org/10.1111/tpj.70396
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intvolume: ' 123'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: Plant Journal
publication_identifier:
eissn:
- 1365-313X
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
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relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated
embryonic and post-embryonic development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 123
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20656'
abstract:
- lang: eng
text: Phytohormone auxin and its directional transport mediate much of the remarkably
plastic development of higher plants. Positive feedback between auxin signaling
and transport is a prerequisite for (1) self-organizing processes, including vascular
tissue formation, and (2) directional growth responses such as gravitropism. Here,
we identify a mechanism by which auxin signaling directly targets PIN auxin transporters.
Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1)
receptor module, auxin rapidly induces phosphorylation and thus stabilization
of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation
of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of
PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin
flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly
with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback
between cell-surface auxin signaling and PIN-mediated polar auxin transport is
fundamental for robust root gravitropism and presumably for other self-organizing
developmental phenomena.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We gratefully acknowledge Tongda Xu for experimental, material, and
conceptual support. We thank William Gray for providing material, Nataliia Gnyliukh
and Ema Cervenova for help with manuscript preparation, and Julia Schmid for help
with cloning. We thank Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions
and support with phospho-proteomic analyses. We thank the Bioimaging and Life Science
facilities at the Institute of Science and Technology Austria (ISTA) for their excellent
service and assistance. The research leading to these results has received funding
from the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF;
I 6123-B) to J.F., and Y.J. was funded by ERC no. 3363360-APPL under FP/2007-2013.
L.R. was supported by the FP7-PEOPLE-2011-COFUND ISTFELLOW program (IC1023FELL01)
and the European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship
(ALTF 985-2016). S.T. was supported by the National Natural Science Foundation of
China (32321001, 32570366). The work of J.H. was supported by the project JG_2024_003
implemented within the Palacký University Young Researcher Grant.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Dmitrii
full_name: Vladimirtsev, Dmitrii
id: 60466724-5355-11ee-ae5a-fa55e8f99c3d
last_name: Vladimirtsev
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Yongfan
full_name: Yu, Yongfan
last_name: Yu
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Meng
full_name: Chen, Meng
last_name: Chen
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Maria Mar
full_name: Marques-Bueno, Maria Mar
last_name: Marques-Bueno
- first_name: Zainab
full_name: Quddoos, Zainab
id: 32ff3c64-04a0-11f0-a50f-d0c45bfac466
last_name: Quddoos
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Yvon
full_name: Jaillais, Yvon
last_name: Jaillais
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin
signaling targets PIN2-mediated auxin fluxes for root gravitropism. Cell.
2025;188(22):6138-6150.e17. doi:10.1016/j.cell.2025.08.026
apa: Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev,
D., … Friml, J. (2025). ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated
auxin fluxes for root gravitropism. Cell. Elsevier. https://doi.org/10.1016/j.cell.2025.08.026
chicago: Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini,
Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface
Auxin Signaling Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell.
Elsevier, 2025. https://doi.org/10.1016/j.cell.2025.08.026.
ieee: L. Rodriguez Solovey et al., “ABP1/ABL3-TMK1 cell-surface auxin signaling
targets PIN2-mediated auxin fluxes for root gravitropism,” Cell, vol. 188,
no. 22. Elsevier, p. 6138–6150.e17, 2025.
ista: Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev
D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer
L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Kulich I, Jaillais Y, Friml J.
2025. ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin
fluxes for root gravitropism. Cell. 188(22), 6138–6150.e17.
mla: Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling
Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” Cell, vol. 188,
no. 22, Elsevier, 2025, p. 6138–6150.e17, doi:10.1016/j.cell.2025.08.026.
short: L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev,
M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer,
L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, I. Kulich, Y. Jaillais, J. Friml,
Cell 188 (2025) 6138–6150.e17.
corr_author: '1'
date_created: 2025-11-19T09:44:31Z
date_published: 2025-10-30T00:00:00Z
date_updated: 2026-07-06T12:51:13Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
- _id: XiFe
doi: 10.1016/j.cell.2025.08.026
ec_funded: 1
external_id:
isi:
- '001616077900005'
pmid:
- '41043433'
file:
- access_level: open_access
checksum: 8ac396a0806ad7f2e4e7a0c1eed712ce
content_type: application/pdf
creator: dernst
date_created: 2025-11-24T10:55:18Z
date_updated: 2025-11-24T10:55:18Z
file_id: '20679'
file_name: 2025_Cell_Rodriguez.pdf
file_size: 17825465
relation: main_file
success: 1
file_date_updated: 2025-11-24T10:55:18Z
fulldoi: https://doi.org/10.1016/j.cell.2025.08.026
has_accepted_license: '1'
intvolume: ' 188'
isi: 1
issue: '22'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 6138-6150.e17
pmid: 1
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 26060676-B435-11E9-9278-68D0E5697425
grant_number: ALTF 985-2016
name: Cell surface receptor complexes for auxin signaling in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Cell
publication_identifier:
issn:
- 0092-8674
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '19399'
relation: earlier_version
status: public
status: public
title: ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes
for root gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 188
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '19399'
abstract:
- lang: eng
text: Phytohormone auxin and its directional transport mediate much of the remarkably
plastic development of higher plants. Positive feedback between auxin signaling
and transport is a key prerequisite for (i) self-organizing processes including
vascular tissue formation and (ii) directional growth responses such as gravitropism.
Here we identify a mechanism, by which auxin signaling directly targets PIN auxin
transporters. Via the cell-surface ABP1-TMK1 receptor module, auxin rapidly induces
phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial
auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces
TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower
root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of
TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE
auxin receptor ABL3. Such positive feedback between cell-surface auxin signaling
and PIN-mediated polar auxin transport is fundamental for robust root gravitropism
and presumably also for other self-organizing developmental phenomena.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We thank W. Gray for providing material; N. Gnyliukh and E. Cervenova
for help with manuscript preparation; J. Schmid for help with cloning. We thank
Dolf Weijers, Mark Roosjen, and Andre Kuhn for discussions and support with phospho-proteomic
analyses. We thank the Bioimaging and Life Science facilities at ISTA for their
excellent service and assistance. The research leading to these results has received
funding from the European Research Council (ERC) under the European Union’s Horizon
2020 research and innovation program grant agreement No 742985 and Austrian Science
Fund (FWF): I3630-775 B25 to J.F; National Natural Science Foundation of China (Grant
32130010, 31422008), start-up funds from FAFU to T.X., Y.J. was funded by ERC no.
3363360-APPL under FP/2007-2013. L.R. was supported by FP7-PEOPLE-2011-COFUND ISTFELLOW
program (IC1023FELL01) and the European Molecular Biology Organization (EMBO) long-term
postdoctoral fellowship (ALTF 985- 2016). S.T. was supported by the National Natural
Science Foundation of China (32321001).'
article_processing_charge: No
author:
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Dmitrii
full_name: Vladimirtsev, Dmitrii
id: 60466724-5355-11ee-ae5a-fa55e8f99c3d
last_name: Vladimirtsev
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Yongfan
full_name: Yu, Yongfan
last_name: Yu
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Meng
full_name: Chen, Meng
last_name: Chen
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Maria Mar
full_name: Marques-Bueno, Maria Mar
last_name: Marques-Bueno
- first_name: Zainab
full_name: Quddoos, Zainab
id: 32ff3c64-04a0-11f0-a50f-d0c45bfac466
last_name: Quddoos
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Yvon
full_name: Jaillais, Yvon
last_name: Jaillais
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rodriguez Solovey L, Fiedler L, Zou M, et al. ABP1/ABL3-TMK1 cell-surface auxin
signaling directly targets PIN2-mediated auxin fluxes for root gravitropism. bioRxiv.
doi:10.1101/2022.11.30.518503
apa: Rodriguez Solovey, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Vladimirtsev,
D., … Friml, J. (n.d.). ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets
PIN2-mediated auxin fluxes for root gravitropism. bioRxiv. https://doi.org/10.1101/2022.11.30.518503
chicago: Rodriguez Solovey, Lesia, Lukas Fiedler, Minxia Zou, Caterina Giannini,
Aline Monzer, Dmitrii Vladimirtsev, Marek Randuch, et al. “ABP1/ABL3-TMK1 Cell-Surface
Auxin Signaling Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.”
BioRxiv, n.d. https://doi.org/10.1101/2022.11.30.518503.
ieee: L. Rodriguez Solovey et al., “ABP1/ABL3-TMK1 cell-surface auxin signaling
directly targets PIN2-mediated auxin fluxes for root gravitropism,” bioRxiv.
.
ista: Rodriguez Solovey L, Fiedler L, Zou M, Giannini C, Monzer A, Vladimirtsev
D, Randuch M, Yu Y, Gelová Z, Verstraeten I, Hajny J, Chen M, Tan S, Hörmayer
L, Li L, Marques-Bueno MM, Quddoos Z, Molnar G, Xu T, Kulich I, Jaillais Y, Friml
J. ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated
auxin fluxes for root gravitropism. bioRxiv, 10.1101/2022.11.30.518503.
mla: Rodriguez Solovey, Lesia, et al. “ABP1/ABL3-TMK1 Cell-Surface Auxin Signaling
Directly Targets PIN2-Mediated Auxin Fluxes for Root Gravitropism.” BioRxiv,
doi:10.1101/2022.11.30.518503.
short: L. Rodriguez Solovey, L. Fiedler, M. Zou, C. Giannini, A. Monzer, D. Vladimirtsev,
M. Randuch, Y. Yu, Z. Gelová, I. Verstraeten, J. Hajny, M. Chen, S. Tan, L. Hörmayer,
L. Li, M.M. Marques-Bueno, Z. Quddoos, G. Molnar, T. Xu, I. Kulich, Y. Jaillais,
J. Friml, BioRxiv (n.d.).
corr_author: '1'
das_tickbox: '1'
date_created: 2025-03-13T08:36:48Z
date_published: 2025-02-20T00:00:00Z
date_updated: 2026-08-14T09:33:45Z
day: '20'
ddc:
- '580'
department:
- _id: JiFr
- _id: XiFe
doi: 10.1101/2022.11.30.518503
ec_funded: 1
fulldoi: https://doi.org/10.1101/2022.11.30.518503
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2022.11.30.518503
month: '02'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 26060676-B435-11E9-9278-68D0E5697425
grant_number: ALTF 985-2016
name: Cell surface receptor complexes for auxin signaling in plants
publication: bioRxiv
publication_status: draft
related_material:
record:
- id: '20656'
relation: later_version
status: public
- id: '20364'
relation: dissertation_contains
status: public
- id: '19395'
relation: dissertation_contains
status: public
status: public
title: ABP1/ABL3-TMK1 cell-surface auxin signaling directly targets PIN2-mediated
auxin fluxes for root gravitropism
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
_id: '14447'
abstract:
- lang: eng
text: "Auxin belongs among major phytohormones and governs multiple aspects of plant
growth and development. The establishment of auxin concentration gradients, determines,
among other processes, plant organ positioning and growth responses to environmental
stimuli.\r\nHerein we report the synthesis of new NBD- or DNS-labelled IAA derivatives
and the elucidation of their biological activity, fluorescence properties and
subcellular accumulation patterns in planta. These novel compounds did not show
auxin-like activity, but instead antagonized physiological auxin effects. The
DNS-labelled derivatives FL5 and FL6 showed strong anti-auxin activity in roots
and hypocotyls, which also occurred at the level of gene transcription as confirmed
by quantitative PCR analysis. The auxin antagonism of our derivatives was further
demonstrated in vitro using an SPR-based binding assay. The NBD-labelled compound
FL4 with the best fluorescence properties proved to be unsuitable to study auxin
accumulation patterns in planta. On the other hand, the strongest anti-auxin activity
possessing compounds FL5 and FL6 could be useful to study binding mechanisms to
auxin receptors and for manipulations of auxin-regulated processes."
acknowledgement: The authors would like to thank Karolína Kubiasová and Iñigo Saiz-Fernández
for valuable scientific discussions. Open access publishing supported by the National
Technical Library in Prague. This work was supported by the Palacký University Olomouc
Young Researcher Grant Competition (JG_2020_002), by the Internal Grant Agency of
Palacký University Olomouc (IGA_PrF_2023_016, IGA_PrF_2023_031), by the Ministry
of Education, Youth and Sports of the Czech Republic through the European Regional
Development Fund-Project Plants as a tool for sustainable global development (CZ.02.1.01/0.0/0.0/16_019/0000827)
and the project Support of mobility at Palacký University Olomouc II. (CZ.02.2.69/0.0/0.0/18_053/0016919).
The Biacore T200 SPR instrument was provided by the WISB Research Technology Facility
within the School of Life Sciences, University of Warwick.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Kristýna
full_name: Bieleszová, Kristýna
last_name: Bieleszová
- first_name: Pavel
full_name: Hladík, Pavel
last_name: Hladík
- first_name: Martin
full_name: Kubala, Martin
last_name: Kubala
- first_name: Richard
full_name: Napier, Richard
last_name: Napier
- first_name: Federica
full_name: Brunoni, Federica
last_name: Brunoni
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Ivan
full_name: Kulich, Ivan
id: 57a1567c-8314-11eb-9063-c9ddc3451a54
last_name: Kulich
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Karel
full_name: Doležal, Karel
last_name: Doležal
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Asta
full_name: Žukauskaitė, Asta
last_name: Žukauskaitė
citation:
ama: 'Bieleszová K, Hladík P, Kubala M, et al. New fluorescent auxin derivatives:
Anti-auxin activity and accumulation patterns in Arabidopsis thaliana. Plant
Growth Regulation. 2024;102:589-602. doi:10.1007/s10725-023-01083-0'
apa: 'Bieleszová, K., Hladík, P., Kubala, M., Napier, R., Brunoni, F., Gelová, Z.,
… Žukauskaitė, A. (2024). New fluorescent auxin derivatives: Anti-auxin activity
and accumulation patterns in Arabidopsis thaliana. Plant Growth Regulation.
Springer Nature. https://doi.org/10.1007/s10725-023-01083-0'
chicago: 'Bieleszová, Kristýna, Pavel Hladík, Martin Kubala, Richard Napier, Federica
Brunoni, Zuzana Gelová, Lukas Fiedler, et al. “New Fluorescent Auxin Derivatives:
Anti-Auxin Activity and Accumulation Patterns in Arabidopsis Thaliana.” Plant
Growth Regulation. Springer Nature, 2024. https://doi.org/10.1007/s10725-023-01083-0.'
ieee: 'K. Bieleszová et al., “New fluorescent auxin derivatives: Anti-auxin
activity and accumulation patterns in Arabidopsis thaliana,” Plant Growth Regulation,
vol. 102. Springer Nature, pp. 589–602, 2024.'
ista: 'Bieleszová K, Hladík P, Kubala M, Napier R, Brunoni F, Gelová Z, Fiedler
L, Kulich I, Strnad M, Doležal K, Novák O, Friml J, Žukauskaitė A. 2024. New fluorescent
auxin derivatives: Anti-auxin activity and accumulation patterns in Arabidopsis
thaliana. Plant Growth Regulation. 102, 589–602.'
mla: 'Bieleszová, Kristýna, et al. “New Fluorescent Auxin Derivatives: Anti-Auxin
Activity and Accumulation Patterns in Arabidopsis Thaliana.” Plant Growth Regulation,
vol. 102, Springer Nature, 2024, pp. 589–602, doi:10.1007/s10725-023-01083-0.'
short: K. Bieleszová, P. Hladík, M. Kubala, R. Napier, F. Brunoni, Z. Gelová, L.
Fiedler, I. Kulich, M. Strnad, K. Doležal, O. Novák, J. Friml, A. Žukauskaitė,
Plant Growth Regulation 102 (2024) 589–602.
date_created: 2023-10-22T22:01:15Z
date_published: 2024-04-01T00:00:00Z
date_updated: 2024-07-16T08:13:39Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1007/s10725-023-01083-0
external_id:
isi:
- '001084334300001'
file:
- access_level: open_access
checksum: e63271b1d57c1f03c1d993fd62cba59c
content_type: application/pdf
creator: dernst
date_created: 2024-07-16T08:13:24Z
date_updated: 2024-07-16T08:13:24Z
file_id: '17252'
file_name: 2024_PlantGrowthReg_Bieleszova.pdf
file_size: 2847929
relation: main_file
success: 1
file_date_updated: 2024-07-16T08:13:24Z
fulldoi: https://doi.org/10.1007/s10725-023-01083-0
has_accepted_license: '1'
intvolume: ' 102'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 589-602
publication: Plant Growth Regulation
publication_identifier:
eissn:
- 1573-5087
issn:
- 0167-6903
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'New fluorescent auxin derivatives: Anti-auxin activity and accumulation patterns
in Arabidopsis thaliana'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 102
year: '2024'
...
---
_id: '14313'
abstract:
- lang: eng
text: To respond to auxin, the chief orchestrator of their multicellularity, plants
evolved multiple receptor systems and signal transduction cascades. Despite decades
of research, however, we are still lacking a satisfactory synthesis of various
auxin signaling mechanisms. The chief discrepancy and historical controversy of
the field is that of rapid and slow auxin effects on plant physiology and development.
How is it possible that ions begin to trickle across the plasma membrane as soon
as auxin enters the cell, even though the best-characterized transcriptional auxin
pathway can take effect only after tens of minutes? Recently, unexpected progress
has been made in understanding this and other unknowns of auxin signaling. We
provide a perspective on these exciting developments and concepts whose general
applicability might have ramifications beyond auxin signaling.
acknowledgement: The opening quote is not intended to reflect any political views
of the authors. The authors by no means endorse the rhetoric of Donald Rumsfeld
or the 2003 invasion of Iraq by the United States. Nevertheless, Rumsfeld's quote
led to both public and academic debates on the concept of known and unknown unknowns,
which can be applied to the recent unexpected developments in the auxin signaling
field. We thank Linlin Qi and Huihuang Chen for their suggestions on figure presentation
and inspiring discussions of TIR1/AFB signaling. Finally, we thank Aroosa Hussain
for discussion of Greek mythology.
article_number: '102443'
article_processing_charge: No
article_type: review
author:
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Fiedler L, Friml J. Rapid auxin signaling: Unknowns old and new. Current
Opinion in Plant Biology. 2023;75(10). doi:10.1016/j.pbi.2023.102443'
apa: 'Fiedler, L., & Friml, J. (2023). Rapid auxin signaling: Unknowns old and
new. Current Opinion in Plant Biology. Elsevier. https://doi.org/10.1016/j.pbi.2023.102443'
chicago: 'Fiedler, Lukas, and Jiří Friml. “Rapid Auxin Signaling: Unknowns Old and
New.” Current Opinion in Plant Biology. Elsevier, 2023. https://doi.org/10.1016/j.pbi.2023.102443.'
ieee: 'L. Fiedler and J. Friml, “Rapid auxin signaling: Unknowns old and new,” Current
Opinion in Plant Biology, vol. 75, no. 10. Elsevier, 2023.'
ista: 'Fiedler L, Friml J. 2023. Rapid auxin signaling: Unknowns old and new. Current
Opinion in Plant Biology. 75(10), 102443.'
mla: 'Fiedler, Lukas, and Jiří Friml. “Rapid Auxin Signaling: Unknowns Old and New.”
Current Opinion in Plant Biology, vol. 75, no. 10, 102443, Elsevier, 2023,
doi:10.1016/j.pbi.2023.102443.'
short: L. Fiedler, J. Friml, Current Opinion in Plant Biology 75 (2023).
corr_author: '1'
date_created: 2023-09-10T22:01:11Z
date_published: 2023-10-01T00:00:00Z
date_updated: 2025-09-09T12:54:16Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2023.102443
external_id:
isi:
- '001080095300001'
pmid:
- '37666097'
file:
- access_level: open_access
checksum: 1c476c3414d2dfb0c85db0cb6cfd8a28
content_type: application/pdf
creator: amally
date_created: 2023-11-02T17:03:20Z
date_updated: 2023-11-02T17:03:20Z
file_id: '14482'
file_name: Fiedler CurrOpinOlantBiol 2023_revised.pdf
file_size: 737872
relation: main_file
success: 1
file_date_updated: 2023-11-02T17:03:20Z
fulldoi: https://doi.org/10.1016/j.pbi.2023.102443
has_accepted_license: '1'
intvolume: ' 75'
isi: 1
issue: '10'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Rapid auxin signaling: Unknowns old and new'
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 75
year: '2023'
...
---
_id: '12291'
abstract:
- lang: eng
text: The phytohormone auxin triggers transcriptional reprogramming through a well-characterized
perception machinery in the nucleus. By contrast, mechanisms that underlie fast
effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation
of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding
protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4.
Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds
auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its
plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required
for the auxin-induced ultrafast global phospho-response and for downstream processes
that include the activation of H+-ATPase and accelerated cytoplasmic streaming.
abp1 and tmk mutants cannot establish auxin-transporting channels and show defective
auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that
lacks the capacity to bind auxin is unable to complement these defects in abp1
mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface
signalling, which mediates the global phospho-response and auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We acknowledge K. Kubiasová for excellent technical assistance, J.
Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production
and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI;
and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the
Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM
(MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F.
is grateful to R. Napier for many insightful suggestions and support. We thank all
past and present members of the Friml group for their support and for other contributions
to this effort to clarify the controversial role of ABP1 over the past seven years.
The project received funding from the European Research Council (ERC) under the
European Union’s Horizon 2020 research and innovation program (grant agreement no.
742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.);
the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001
to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science
and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053
to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and
20H05910).
article_processing_charge: No
article_type: original
author:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Branka D.
full_name: Živanović, Branka D.
last_name: Živanović
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Peter
full_name: Grones, Peter
last_name: Grones
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Andre
full_name: Kuhn, Andre
last_name: Kuhn
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Nikola
full_name: Rýdza, Nikola
last_name: Rýdza
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Anastasiia
full_name: Teplova, Anastasiia
id: e3736151-106c-11ec-b916-c2558e2762c6
last_name: Teplova
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
citation:
ama: Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation
and auxin canalization. Nature. 2022;609(7927):575-581. doi:10.1038/s41586-022-05187-x
apa: Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A.,
… Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and
auxin canalization. Nature. Springer Nature. https://doi.org/10.1038/s41586-022-05187-x
chicago: Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa
Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception
for Global Phosphorylation and Auxin Canalization.” Nature. Springer Nature,
2022. https://doi.org/10.1038/s41586-022-05187-x.
ieee: J. Friml et al., “ABP1–TMK auxin perception for global phosphorylation
and auxin canalization,” Nature, vol. 609, no. 7927. Springer Nature, pp.
575–581, 2022.
ista: Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey
L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones
P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K,
Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception
for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.
mla: Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and
Auxin Canalization.” Nature, vol. 609, no. 7927, Springer Nature, 2022,
pp. 575–81, doi:10.1038/s41586-022-05187-x.
short: J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez
Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini,
P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza,
K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature
609 (2022) 575–581.
corr_author: '1'
date_created: 2023-01-16T10:04:48Z
date_published: 2022-09-15T00:00:00Z
date_updated: 2026-08-14T09:33:45Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: EvBe
- _id: EM-Fac
doi: 10.1038/s41586-022-05187-x
ec_funded: 1
external_id:
isi:
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pmid:
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language:
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month: '09'
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project:
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call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Nature
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title: ABP1–TMK auxin perception for global phosphorylation and auxin canalization
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 609
year: '2022'
...