---
OA_place: publisher
OA_type: hybrid
_id: '18807'
abstract:
- lang: eng
  text: Developing tissues interpret dynamic changes in morphogen activity to generate
    cell type diversity. To quantitatively study bone morphogenetic protein (BMP)
    signaling dynamics in the mouse neural tube, we developed an embryonic stem cell
    differentiation system tailored for growing tissues. Differentiating cells form
    striking self-organized patterns of dorsal neural tube cell types driven by sequential
    phases of BMP signaling that are observed both in vitro and in vivo. Data-driven
    biophysical modeling showed that these dynamics result from coupling fast negative
    feedback with slow positive regulation of signaling by the specification of an
    endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling
    in space, we identify a BMP signaling relay that operates in time. This mechanism
    allows for a rapid initial concentration-sensitive response that is robustly terminated,
    thereby regulating balanced sequential cell type generation. Our study provides
    an experimental and theoretical framework to understand how signaling dynamics
    are exploited in developing tissues.
acknowledgement: We thank A. Miller and N. Papalopulu for reagents and J. Briscoe
  for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European
  Research Council under Horizon Europe, grant 101044579; and the Austrian Science
  Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft
  für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was
  supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral
  Fellowship (ALTF 343-2022).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Stefanie
  full_name: Rus, Stefanie
  id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
  last_name: Rus
  orcid: 0000-0001-8703-1093
- first_name: David
  full_name: Brückner, David
  id: e1e86031-6537-11eb-953a-f7ab92be508d
  last_name: Brückner
  orcid: 0000-0001-7205-2975
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Martina
  full_name: Greunz, Martina
  id: 48A59534-F248-11E8-B48F-1D18A9856A87
  last_name: Greunz
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
citation:
  ama: Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in
    the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental
    Cell</i>. 2025;60(4):567-580. doi:<a href="https://doi.org/10.1016/j.devcel.2024.10.024">10.1016/j.devcel.2024.10.024</a>
  apa: Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E.
    B., &#38; Kicheva, A. (2025). Self-organized pattern formation in the developing
    mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.devcel.2024.10.024">https://doi.org/10.1016/j.devcel.2024.10.024</a>
  chicago: Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack
    Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation
    in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental
    Cell</i>. Elsevier, 2025. <a href="https://doi.org/10.1016/j.devcel.2024.10.024">https://doi.org/10.1016/j.devcel.2024.10.024</a>.
  ieee: S. Rus <i>et al.</i>, “Self-organized pattern formation in the developing
    mouse neural tube by a temporal relay of BMP signaling,” <i>Developmental Cell</i>,
    vol. 60, no. 4. Elsevier, pp. 567–580, 2025.
  ista: Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva
    A. 2025. Self-organized pattern formation in the developing mouse neural tube
    by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.
  mla: Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse
    Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>,
    vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:<a href="https://doi.org/10.1016/j.devcel.2024.10.024">10.1016/j.devcel.2024.10.024</a>.
  short: S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo,
    A. Kicheva, Developmental Cell 60 (2025) 567–580.
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: RNA-seq data has been deposited at GEO and are publicly
  available as of the date of publication. Accession numbers are listed in the key
  resources table. Code for the quantification of fluorescence intensity (FI) profiles
  in differentiated cells on stencils, as well as for model simulations, is available
  on GitHub (https://github.com/dbrueckner/NeuralTubeColonies, https://doi.org/10.5281/zenodo.13335390
date_created: 2025-01-09T11:25:47Z
date_published: 2025-02-24T00:00:00Z
date_updated: 2026-10-07T22:31:21Z
day: '24'
ddc:
- '570'
department:
- _id: AnKi
- _id: EdHa
- _id: NanoFab
doi: 10.1016/j.devcel.2024.10.024
external_id:
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  - '001434279000001'
  pmid:
  - '39603235'
file:
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  date_created: 2025-04-16T10:54:07Z
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has_accepted_license: '1'
intvolume: '        60'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 567-580
pmid: 1
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
  grant_number: '101044579'
  name: Mechanisms of tissue size regulation in spinal cord development
- _id: 059DF620-7A3F-11EA-A408-12923DDC885E
  grant_number: F7802
  name: Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen
    control of growth and pattern in the spinal cord
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
  grant_number: SC19-011
  name: The regulatory logic of pattern formation in the vertebrate dorsal neural
    tube
publication: Developmental Cell
publication_identifier:
  issn:
  - 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
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researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Self-organized pattern formation in the developing mouse neural tube by a temporal
  relay of BMP signaling
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 60
year: '2025'
...
---
APC_amount: 804 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18601'
abstract:
- lang: eng
  text: "Geometrically controlled stem cell differentiation promotes reproducible
    pattern formation. Here, we present a protocol to fabricate elastomeric stencils
    for patterned stem cell differentiation. We describe procedures for using photolithography
    to produce molds, followed by molding polydimethylsiloxane (PDMS) to obtain stencils
    with through holes. We then provide instructions for culturing cells on stencils
    and, finally, removing stencils to allow colony growth and cell migration. This
    approach yields reproducible two-dimensional organoids tailored for quantitative
    studies of growth and pattern formation.\r\nFor complete details on the use and
    execution of this protocol, please refer to Lehr et al.1"
acknowledged_ssus:
- _id: NanoFab
acknowledgement: We thank the nanofabrication facility at ISTA for technical assistance.
  Work in the A.K. lab is supported by ISTA, the European Research Council under Horizon
  Europe (grant 101044579), and the Austrian Science Fund (FWF) (grant https://doi.org/10.55776/F78).
  S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H.
  fellowship SC19-011.
article_number: '103187'
article_processing_charge: Yes
article_type: original
author:
- first_name: Stefanie
  full_name: Rus, Stefanie
  id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
  last_name: Rus
  orcid: 0000-0001-8703-1093
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Monika Aleksandra
  full_name: Kulig, Monika Aleksandra
  id: 3331f5ae-e896-11ec-af79-eeb79769bcb7
  last_name: Kulig
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
citation:
  ama: Rus S, Merrin J, Kulig MA, Minchington T, Kicheva A. Protocol for fabricating
    elastomeric stencils for patterned stem cell differentiation. <i>STAR Protocols</i>.
    2024;5(4). doi:<a href="https://doi.org/10.1016/j.xpro.2024.103187">10.1016/j.xpro.2024.103187</a>
  apa: Rus, S., Merrin, J., Kulig, M. A., Minchington, T., &#38; Kicheva, A. (2024).
    Protocol for fabricating elastomeric stencils for patterned stem cell differentiation.
    <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2024.103187">https://doi.org/10.1016/j.xpro.2024.103187</a>
  chicago: Rus, Stefanie, Jack Merrin, Monika Aleksandra Kulig, Thomas Minchington,
    and Anna Kicheva. “Protocol for Fabricating Elastomeric Stencils for Patterned
    Stem Cell Differentiation.” <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2024.103187">https://doi.org/10.1016/j.xpro.2024.103187</a>.
  ieee: S. Rus, J. Merrin, M. A. Kulig, T. Minchington, and A. Kicheva, “Protocol
    for fabricating elastomeric stencils for patterned stem cell differentiation,”
    <i>STAR Protocols</i>, vol. 5, no. 4. Elsevier, 2024.
  ista: Rus S, Merrin J, Kulig MA, Minchington T, Kicheva A. 2024. Protocol for fabricating
    elastomeric stencils for patterned stem cell differentiation. STAR Protocols.
    5(4), 103187.
  mla: Rus, Stefanie, et al. “Protocol for Fabricating Elastomeric Stencils for Patterned
    Stem Cell Differentiation.” <i>STAR Protocols</i>, vol. 5, no. 4, 103187, Elsevier,
    2024, doi:<a href="https://doi.org/10.1016/j.xpro.2024.103187">10.1016/j.xpro.2024.103187</a>.
  short: S. Rus, J. Merrin, M.A. Kulig, T. Minchington, A. Kicheva, STAR Protocols
    5 (2024).
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: This study did not generate datasets or code.
date_created: 2024-12-01T23:01:53Z
date_published: 2024-12-20T00:00:00Z
date_updated: 2026-10-07T22:31:21Z
day: '20'
ddc:
- '570'
department:
- _id: AnKi
- _id: NanoFab
doi: 10.1016/j.xpro.2024.103187
external_id:
  pmid:
  - '39602310'
file:
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  checksum: 0c61a6f9978608a103865905e06f4581
  content_type: application/pdf
  creator: dernst
  date_created: 2024-12-03T10:53:23Z
  date_updated: 2024-12-03T10:53:23Z
  file_id: '18610'
  file_name: 2024_STARProtoc_Lehr.pdf
  file_size: 4989169
  relation: main_file
  success: 1
file_date_updated: 2024-12-03T10:53:23Z
fulldoi: https://doi.org/10.1016/j.xpro.2024.103187
has_accepted_license: '1'
intvolume: '         5'
issue: '4'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
  grant_number: '101044579'
  name: Mechanisms of tissue size regulation in spinal cord development
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
  grant_number: SC19-011
  name: The regulatory logic of pattern formation in the vertebrate dorsal neural
    tube
publication: STAR Protocols
publication_identifier:
  eissn:
  - 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '19763'
    relation: dissertation_contains
    status: public
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: Protocol for fabricating elastomeric stencils for patterned stem cell differentiation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 5
year: '2024'
...
---
_id: '13136'
abstract:
- lang: eng
  text: Despite its fundamental importance for development, the question of how organs
    achieve their correct size and shape is poorly understood. This complex process
    requires coordination between the generation of cell mass and the morphogenetic
    mechanisms that sculpt tissues. These processes are regulated by morphogen signalling
    pathways and mechanical forces. Yet, in many systems, it is unclear how biochemical
    and mechanical signalling are quantitatively interpreted to determine the behaviours
    of individual cells and how they contribute to growth and morphogenesis at the
    tissue scale. In this review, we discuss the development of the vertebrate neural
    tube and somites as an example of the state of knowledge, as well as the challenges
    in understanding the mechanisms of tissue size control in vertebrate organogenesis.
    We highlight how the recent advances in stem cell differentiation and organoid
    approaches can be harnessed to provide new insights into this question.
acknowledgement: 'We thank J. Briscoe for comments on the manuscript. Work in the
  AK lab is supported by ISTA, the European Research Council under Horizon Europe:
  grant 101044579, and Austrian Science Fund (FWF): F78 (Stem Cell Modulation). SR
  is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship
  SC19-011.'
article_number: '100459'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Stefanie
  full_name: Rus, Stefanie
  id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
  last_name: Rus
  orcid: 0000-0001-8703-1093
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
citation:
  ama: Minchington T, Rus S, Kicheva A. Control of tissue dimensions in the developing
    neural tube and somites. <i>Current Opinion in Systems Biology</i>. 2023;35. doi:<a
    href="https://doi.org/10.1016/j.coisb.2023.100459">10.1016/j.coisb.2023.100459</a>
  apa: Minchington, T., Rus, S., &#38; Kicheva, A. (2023). Control of tissue dimensions
    in the developing neural tube and somites. <i>Current Opinion in Systems Biology</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.coisb.2023.100459">https://doi.org/10.1016/j.coisb.2023.100459</a>
  chicago: Minchington, Thomas, Stefanie Rus, and Anna Kicheva. “Control of Tissue
    Dimensions in the Developing Neural Tube and Somites.” <i>Current Opinion in Systems
    Biology</i>. Elsevier, 2023. <a href="https://doi.org/10.1016/j.coisb.2023.100459">https://doi.org/10.1016/j.coisb.2023.100459</a>.
  ieee: T. Minchington, S. Rus, and A. Kicheva, “Control of tissue dimensions in the
    developing neural tube and somites,” <i>Current Opinion in Systems Biology</i>,
    vol. 35. Elsevier, 2023.
  ista: Minchington T, Rus S, Kicheva A. 2023. Control of tissue dimensions in the
    developing neural tube and somites. Current Opinion in Systems Biology. 35, 100459.
  mla: Minchington, Thomas, et al. “Control of Tissue Dimensions in the Developing
    Neural Tube and Somites.” <i>Current Opinion in Systems Biology</i>, vol. 35,
    100459, Elsevier, 2023, doi:<a href="https://doi.org/10.1016/j.coisb.2023.100459">10.1016/j.coisb.2023.100459</a>.
  short: T. Minchington, S. Rus, A. Kicheva, Current Opinion in Systems Biology 35
    (2023).
corr_author: '1'
date_created: 2023-06-18T22:00:46Z
date_published: 2023-09-01T00:00:00Z
date_updated: 2026-10-07T22:31:20Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1016/j.coisb.2023.100459
file:
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  date_created: 2024-01-29T11:06:45Z
  date_updated: 2024-01-29T11:06:45Z
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  file_name: 2023_CurrOpSystBioloy_Minchington.pdf
  file_size: 598842
  relation: main_file
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file_date_updated: 2024-01-29T11:06:45Z
fulldoi: https://doi.org/10.1016/j.coisb.2023.100459
has_accepted_license: '1'
intvolume: '        35'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
  grant_number: '101044579'
  name: Mechanisms of tissue size regulation in spinal cord development
- _id: 059DF620-7A3F-11EA-A408-12923DDC885E
  grant_number: F7802
  name: Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen
    control of growth and pattern in the spinal cord
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
  grant_number: SC19-011
  name: The regulatory logic of pattern formation in the vertebrate dorsal neural
    tube
publication: Current Opinion in Systems Biology
publication_identifier:
  eissn:
  - 2452-3100
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
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  - id: '19763'
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    status: public
scopus_import: '1'
status: public
title: Control of tissue dimensions in the developing neural tube and somites
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
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type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 35
year: '2023'
...
---
_id: '12245'
abstract:
- lang: eng
  text: MicroRNAs (miRs) have an important role in tuning dynamic gene expression.
    However, the mechanism by which they are quantitatively controlled is unknown.
    We show that the amount of mature miR-9, a key regulator of neuronal development,
    increases during zebrafish neurogenesis in a sharp stepwise manner. We characterize
    the spatiotemporal profile of seven distinct microRNA primary transcripts (pri-mir)-9s
    that produce the same mature miR-9 and show that they are sequentially expressed
    during hindbrain neurogenesis. Expression of late-onset pri-mir-9-1 is added on
    to, rather than replacing, the expression of early onset pri-mir-9-4 and -9-5
    in single cells. CRISPR/Cas9 mutation of the late-onset pri-mir-9-1 prevents the
    developmental increase of mature miR-9, reduces late neuronal differentiation
    and fails to downregulate Her6 at late stages. Mathematical modelling shows that
    an adaptive network containing Her6 is insensitive to linear increases in miR-9
    but responds to stepwise increases of miR-9. We suggest that a sharp stepwise
    increase of mature miR-9 is created by sequential and additive temporal activation
    of distinct loci. This may be a strategy to overcome adaptation and facilitate
    a transition of Her6 to a new dynamic regime or steady state.
acknowledgement: "We are grateful to Dr Tom Pettini for the advice on smiFISH technique
  and Dr Laure Bally-Cuif for sharing plasmids. The authors also thank the Biological
  Services Facility, Bioimaging and Systems Microscopy Facilities of the University
  of Manchester for technical support.\r\nThis work was supported by a Wellcome Trust
  Senior Research Fellowship (090868/Z/09/Z) and a Wellcome Trust Investigator Award
  (224394/Z/21/Z) to N.P. and a Medical Research Council Career Development Award
  to C.S.M. (MR/V032534/1). J.B. was supported by a Wellcome Trust Four-Year PhD Studentship
  in Basic Science (219992/Z/19/Z). Open Access funding provided by The University
  of Manchester. Deposited in PMC for immediate release."
article_number: dev200474
article_processing_charge: No
article_type: original
author:
- first_name: Ximena
  full_name: Soto, Ximena
  last_name: Soto
- first_name: Joshua
  full_name: Burton, Joshua
  last_name: Burton
- first_name: Cerys S.
  full_name: Manning, Cerys S.
  last_name: Manning
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Robert
  full_name: Lea, Robert
  last_name: Lea
- first_name: Jessica
  full_name: Lee, Jessica
  last_name: Lee
- first_name: Jochen
  full_name: Kursawe, Jochen
  last_name: Kursawe
- first_name: Magnus
  full_name: Rattray, Magnus
  last_name: Rattray
- first_name: Nancy
  full_name: Papalopulu, Nancy
  last_name: Papalopulu
citation:
  ama: Soto X, Burton J, Manning CS, et al. Sequential and additive expression of
    miR-9 precursors control timing of neurogenesis. <i>Development</i>. 2022;149(19).
    doi:<a href="https://doi.org/10.1242/dev.200474">10.1242/dev.200474</a>
  apa: Soto, X., Burton, J., Manning, C. S., Minchington, T., Lea, R., Lee, J., …
    Papalopulu, N. (2022). Sequential and additive expression of miR-9 precursors
    control timing of neurogenesis. <i>Development</i>. Company of Biologists. <a
    href="https://doi.org/10.1242/dev.200474">https://doi.org/10.1242/dev.200474</a>
  chicago: Soto, Ximena, Joshua Burton, Cerys S. Manning, Thomas Minchington, Robert
    Lea, Jessica Lee, Jochen Kursawe, Magnus Rattray, and Nancy Papalopulu. “Sequential
    and Additive Expression of MiR-9 Precursors Control Timing of Neurogenesis.” <i>Development</i>.
    Company of Biologists, 2022. <a href="https://doi.org/10.1242/dev.200474">https://doi.org/10.1242/dev.200474</a>.
  ieee: X. Soto <i>et al.</i>, “Sequential and additive expression of miR-9 precursors
    control timing of neurogenesis,” <i>Development</i>, vol. 149, no. 19. Company
    of Biologists, 2022.
  ista: Soto X, Burton J, Manning CS, Minchington T, Lea R, Lee J, Kursawe J, Rattray
    M, Papalopulu N. 2022. Sequential and additive expression of miR-9 precursors
    control timing of neurogenesis. Development. 149(19), dev200474.
  mla: Soto, Ximena, et al. “Sequential and Additive Expression of MiR-9 Precursors
    Control Timing of Neurogenesis.” <i>Development</i>, vol. 149, no. 19, dev200474,
    Company of Biologists, 2022, doi:<a href="https://doi.org/10.1242/dev.200474">10.1242/dev.200474</a>.
  short: X. Soto, J. Burton, C.S. Manning, T. Minchington, R. Lea, J. Lee, J. Kursawe,
    M. Rattray, N. Papalopulu, Development 149 (2022).
date_created: 2023-01-16T09:53:17Z
date_published: 2022-10-01T00:00:00Z
date_updated: 2026-08-12T09:59:40Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.200474
external_id:
  isi:
  - '000918161000003'
  pmid:
  - '36189829'
file:
- access_level: open_access
  checksum: d7c29b74e9e4032308228cc704a30e88
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-30T08:35:44Z
  date_updated: 2023-01-30T08:35:44Z
  file_id: '12438'
  file_name: 2022_Development_Soto.pdf
  file_size: 9348839
  relation: main_file
  success: 1
file_date_updated: 2023-01-30T08:35:44Z
fulldoi: https://doi.org/10.1242/dev.200474
has_accepted_license: '1'
intvolume: '       149'
isi: 1
issue: '19'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
  eissn:
  - 1477-9129
  issn:
  - 0950-1991
publication_status: published
publisher: Company of Biologists
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: ' https://github.com/burtonjosh/StepwiseMir9'
scopus_import: '1'
status: public
title: Sequential and additive expression of miR-9 precursors control timing of neurogenesis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 149
year: '2022'
...
---
_id: '15262'
abstract:
- lang: eng
  text: The Hunchback (Hb) transcription factor is crucial for anterior-posterior
    patterning of the Drosophila embryo. The maternal hb mRNA acts as a paradigm for
    translational regulation due to its repression in the posterior of the embryo.
    However, little is known about the translatability of zygotically transcribed
    hb mRNAs. Here, we adapt the SunTag system, developed for imaging translation
    at single-mRNA resolution in tissue culture cells, to the Drosophila embryo to
    study the translation dynamics of zygotic hb mRNAs. Using single-molecule imaging
    in fixed and live embryos, we provide evidence for translational repression of
    zygotic SunTag-hb mRNAs. Whereas the proportion of SunTag-hb mRNAs translated
    is initially uniform, translation declines from the anterior over time until it
    becomes restricted to a posterior band in the expression domain. We discuss how
    regulated hb mRNA translation may help establish the sharp Hb expression boundary,
    which is a model for precision and noise during developmental patterning. Overall,
    our data show how use of the SunTag method on fixed and live embryos is a powerful
    combination for elucidating spatiotemporal regulation of mRNA translation in Drosophila.
article_number: dev196121.
article_processing_charge: No
article_type: original
author:
- first_name: Daisy J.
  full_name: Vinter, Daisy J.
  last_name: Vinter
- first_name: Caroline
  full_name: Hoppe, Caroline
  last_name: Hoppe
- first_name: Thomas
  full_name: Minchington, Thomas
  id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
  last_name: Minchington
- first_name: Catherine
  full_name: Sutcliffe, Catherine
  last_name: Sutcliffe
- first_name: Hilary L.
  full_name: Ashe, Hilary L.
  last_name: Ashe
citation:
  ama: Vinter DJ, Hoppe C, Minchington T, Sutcliffe C, Ashe HL. Dynamics of hunchback
    translation in real-time and at single-mRNA resolution in the Drosophila embryo.
    <i>Development</i>. 2021;148(18). doi:<a href="https://doi.org/10.1242/dev.196121">10.1242/dev.196121</a>
  apa: Vinter, D. J., Hoppe, C., Minchington, T., Sutcliffe, C., &#38; Ashe, H. L.
    (2021). Dynamics of hunchback translation in real-time and at single-mRNA resolution
    in the Drosophila embryo. <i>Development</i>. Company of Biologists. <a href="https://doi.org/10.1242/dev.196121">https://doi.org/10.1242/dev.196121</a>
  chicago: Vinter, Daisy J., Caroline Hoppe, Thomas Minchington, Catherine Sutcliffe,
    and Hilary L. Ashe. “Dynamics of Hunchback Translation in Real-Time and at Single-MRNA
    Resolution in the Drosophila Embryo.” <i>Development</i>. Company of Biologists,
    2021. <a href="https://doi.org/10.1242/dev.196121">https://doi.org/10.1242/dev.196121</a>.
  ieee: D. J. Vinter, C. Hoppe, T. Minchington, C. Sutcliffe, and H. L. Ashe, “Dynamics
    of hunchback translation in real-time and at single-mRNA resolution in the Drosophila
    embryo,” <i>Development</i>, vol. 148, no. 18. Company of Biologists, 2021.
  ista: Vinter DJ, Hoppe C, Minchington T, Sutcliffe C, Ashe HL. 2021. Dynamics of
    hunchback translation in real-time and at single-mRNA resolution in the Drosophila
    embryo. Development. 148(18), dev196121.
  mla: Vinter, Daisy J., et al. “Dynamics of Hunchback Translation in Real-Time and
    at Single-MRNA Resolution in the Drosophila Embryo.” <i>Development</i>, vol.
    148, no. 18, dev196121., Company of Biologists, 2021, doi:<a href="https://doi.org/10.1242/dev.196121">10.1242/dev.196121</a>.
  short: D.J. Vinter, C. Hoppe, T. Minchington, C. Sutcliffe, H.L. Ashe, Development
    148 (2021).
date_created: 2024-04-03T07:26:41Z
date_published: 2021-09-01T00:00:00Z
date_updated: 2026-08-12T09:59:03Z
day: '01'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1242/dev.196121
external_id:
  pmid:
  - '33722899 '
file:
- access_level: open_access
  checksum: 6d0533fe9c712448b3f9feb15e05ec4b
  content_type: application/pdf
  creator: dernst
  date_created: 2024-04-03T13:58:51Z
  date_updated: 2024-04-03T13:58:51Z
  file_id: '15290'
  file_name: 2021_CompanyBiologists_Vinter.pdf
  file_size: 16258500
  relation: main_file
  success: 1
file_date_updated: 2024-04-03T13:58:51Z
fulldoi: https://doi.org/10.1242/dev.196121
has_accepted_license: '1'
intvolume: '       148'
issue: '18'
keyword:
- Developmental Biology
- Molecular Biology
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Development
publication_identifier:
  eissn:
  - 1477-9129
  issn:
  - 0950-1991
publication_status: published
publisher: Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dynamics of hunchback translation in real-time and at single-mRNA resolution
  in the Drosophila embryo
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 148
year: '2021'
...