---
APC_amount: 4215,38 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '19963'
abstract:
- lang: eng
  text: The acquisition of cellular identity requires large-scale alterations in cellular
    state. The noncanonical proteasome activator PSME3 is known to regulate diverse
    cellular processes, but its importance for differentiation remains unclear. Here,
    we demonstrate that PSME3 binds dynamically to highly active promoters over the
    course of differentiation. However, loss of PSME3 does not globally affect mRNA
    transcription. We find instead that PSME3 influences the levels of several adhesion-related
    proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility
    and myoblast differentiation in a proteasome-independent manner. Our findings
    reveal several new facets of PSME3 functionality and highlight its importance
    for the differentiation of myogenic cells.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: 'All proteomics analysis was done by the ISTA LSF Mass Spectrometry
  Service: Ewelina Dutkiewicz-Kopczynska processed the samples (digest and cleanup);
  Bella Bruszel optimized the acquisition methods, acquired the data, and performed
  all searches; and Armel Nicolas provided pre- and post-project consulting and post-processed
  the search results using a development version of their data analysis package, proteoCraft
  (publication pending). The authors would like to thank Saki for their clarity of
  thought and insight, as well as Dr. Lorenzo Puri and the members of his laboratory
  for invaluable discussions relating to the project. This research was further supported
  by the Lab Support Facility and the Imaging and Optics Facility of ISTA.'
article_number: e202503208
article_processing_charge: Yes
article_type: original
author:
- first_name: Kenneth D
  full_name: Kuhn, Kenneth D
  id: 7deed7e0-0133-11f0-8590-c4600b08d0f4
  last_name: Kuhn
- first_name: Ukrae H.
  full_name: Cho, Ukrae H.
  last_name: Cho
- first_name: Martin W
  full_name: Hetzer, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: Hetzer
  orcid: 0000-0002-2111-992X
citation:
  ama: Kuhn KD, Cho UH, Hetzer M. PSME3 regulates migration and differentiation of
    myoblasts. <i>Life Science Alliance</i>. 2025;8(9). doi:<a href="https://doi.org/10.26508/lsa.202503208">10.26508/lsa.202503208</a>
  apa: Kuhn, K. D., Cho, U. H., &#38; Hetzer, M. (2025). PSME3 regulates migration
    and differentiation of myoblasts. <i>Life Science Alliance</i>. Embo Press. <a
    href="https://doi.org/10.26508/lsa.202503208">https://doi.org/10.26508/lsa.202503208</a>
  chicago: Kuhn, Kenneth D, Ukrae H. Cho, and Martin Hetzer. “PSME3 Regulates Migration
    and Differentiation of Myoblasts.” <i>Life Science Alliance</i>. Embo Press, 2025.
    <a href="https://doi.org/10.26508/lsa.202503208">https://doi.org/10.26508/lsa.202503208</a>.
  ieee: K. D. Kuhn, U. H. Cho, and M. Hetzer, “PSME3 regulates migration and differentiation
    of myoblasts,” <i>Life Science Alliance</i>, vol. 8, no. 9. Embo Press, 2025.
  ista: Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation
    of myoblasts. Life Science Alliance. 8(9), e202503208.
  mla: Kuhn, Kenneth D., et al. “PSME3 Regulates Migration and Differentiation of
    Myoblasts.” <i>Life Science Alliance</i>, vol. 8, no. 9, e202503208, Embo Press,
    2025, doi:<a href="https://doi.org/10.26508/lsa.202503208">10.26508/lsa.202503208</a>.
  short: K.D. Kuhn, U.H. Cho, M. Hetzer, Life Science Alliance 8 (2025).
corr_author: '1'
date_created: 2025-07-06T22:01:22Z
date_published: 2025-09-01T00:00:00Z
date_updated: 2026-05-20T08:38:04Z
day: '01'
ddc:
- '570'
department:
- _id: MaHe
doi: 10.26508/lsa.202503208
external_id:
  isi:
  - '001511452100001'
  pmid:
  - '40537284'
file:
- access_level: open_access
  checksum: 591d47aa39fc969986c7d3b966890f5f
  content_type: application/pdf
  creator: dernst
  date_created: 2025-12-30T09:17:09Z
  date_updated: 2025-12-30T09:17:09Z
  file_id: '20904'
  file_name: 2025_LifeScienceAlliance_Kuhn.pdf
  file_size: 5471288
  relation: main_file
  success: 1
file_date_updated: 2025-12-30T09:17:09Z
has_accepted_license: '1'
intvolume: '         8'
isi: 1
issue: '9'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
  name: IST Austria Open Access Fund
publication: Life Science Alliance
publication_identifier:
  eissn:
  - 2575-1077
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: PSME3 regulates migration and differentiation of myoblasts
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2025'
...