---
OA_place: publisher
_id: '20212'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "I would also like to\r\nthank the Austrian Academy of Sciences for
  awarding me a 2-year DOC fellowship\r\n(DOC26253)."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Osvaldo
  full_name: Miranda, Osvaldo
  id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
  last_name: Miranda
  orcid: 0000-0001-6618-6889
citation:
  ama: Miranda O. Unraveling the role of Pten in cortical stem cell lineage progression
    using MADM. 2025. doi:<a href="https://doi.org/10.15479/AT-ISTA-20212">10.15479/AT-ISTA-20212</a>
  apa: Miranda, O. (2025). <i>Unraveling the role of Pten in cortical stem cell lineage
    progression using MADM</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT-ISTA-20212">https://doi.org/10.15479/AT-ISTA-20212</a>
  chicago: Miranda, Osvaldo. “Unraveling the Role of Pten in Cortical Stem Cell Lineage
    Progression Using MADM.” Institute of Science and Technology Austria, 2025. <a
    href="https://doi.org/10.15479/AT-ISTA-20212">https://doi.org/10.15479/AT-ISTA-20212</a>.
  ieee: O. Miranda, “Unraveling the role of Pten in cortical stem cell lineage progression
    using MADM,” Institute of Science and Technology Austria, 2025.
  ista: Miranda O. 2025. Unraveling the role of Pten in cortical stem cell lineage
    progression using MADM. Institute of Science and Technology Austria.
  mla: Miranda, Osvaldo. <i>Unraveling the Role of Pten in Cortical Stem Cell Lineage
    Progression Using MADM</i>. Institute of Science and Technology Austria, 2025,
    doi:<a href="https://doi.org/10.15479/AT-ISTA-20212">10.15479/AT-ISTA-20212</a>.
  short: O. Miranda, Unraveling the Role of Pten in Cortical Stem Cell Lineage Progression
    Using MADM, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-08-22T14:07:00Z
date_published: 2025-08-22T00:00:00Z
date_updated: 2026-04-14T08:16:57Z
day: '22'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SiHi
doi: 10.15479/AT-ISTA-20212
file:
- access_level: closed
  checksum: 3331f76bbef74ff4908e2d2c9262045c
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: omiranda
  date_created: 2025-08-26T09:03:50Z
  date_updated: 2025-08-26T09:03:50Z
  file_id: '20230'
  file_name: 2025_MirandaRomero_OsvaldoAntonio_Thesis.docx
  file_size: 32887334
  relation: source_file
- access_level: closed
  checksum: 02509d50cff8e35c5bcbf71e8d658176
  content_type: application/pdf
  creator: omiranda
  date_created: 2025-08-26T09:05:55Z
  date_updated: 2025-08-26T10:43:30Z
  embargo: 2026-08-26
  embargo_to: open_access
  file_id: '20231'
  file_name: 2025_MirandaRomero_OsvaldoAntonio_Thesis.pdf
  file_size: 28636240
  relation: main_file
file_date_updated: 2025-08-26T10:43:30Z
has_accepted_license: '1'
keyword:
- Pten
- mtor
- cortical development
- MADM
- Mapk
language:
- iso: eng
month: '08'
oa_version: Published Version
page: '119'
project:
- _id: 34c9fbcb-11ca-11ed-8bc3-98fa5658610d
  grant_number: '26253'
  name: Molecular Mechanisms Regulating Cortical Neural Stem Cell Lineage Progression
    and Astrocyte Development
publication_identifier:
  isbn:
  - 978-3-99078-063-3
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '17425'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
title: Unraveling the role of Pten in cortical stem cell lineage progression using
  MADM
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
_id: '17425'
abstract:
- lang: eng
  text: Mosaic Analysis with Double Markers (MADM) is a powerful genetic method typically
    used for lineage tracing and to disentangle cell autonomous and tissue-wide roles
    of candidate genes with single cell resolution. Given the relatively sparse labeling,
    depending on which of the 19 MADM chromosomes one chooses, the MADM approach represents
    the perfect opportunity for cell morphology analysis. Various MADM studies include
    reports of morphological anomalies and phenotypes in the central nervous system
    (CNS). MADM for any candidate gene can easily incorporate morphological analysis
    within the experimental workflow. Here, we describe the methods of morphological
    cell analysis which we developed in the course of diverse recent MADM studies.
    This chapter will specifically focus on methods to quantify aspects of the morphology
    of neurons and astrocytes within the CNS, but these methods can broadly be applied
    to any MADM-labeled cells throughout the entire organism. We will cover two analyses—soma
    volume and dendrite characterization—of physical characteristics of pyramidal
    neurons in the somatosensory cortex, and two analyses—volume and Sholl analysis—of
    astrocyte morphology.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank all Hippenmeyer lab members for support and discussions.
  This work was supported by the Scientific Service Units (SSU) at ISTA through resources
  provided by the Imaging & Optics Facility (IOF). O.A.M was a recipient of a DOC
  Fellowship (26253) of the Austrian Academy of Sciences. This work was supported
  by ISTA institutional funds, and The Austrian Science Fund Special Research Programmes
  (FWF SFB F78 Neuro Stem Modulation) to S.H.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Osvaldo
  full_name: Miranda, Osvaldo
  id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
  last_name: Miranda
  orcid: 0000-0001-6618-6889
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: 'Miranda O, Cheung GT, Hippenmeyer S. Morphological Analysis of Neurons and
    Glia Using Mosaic Analysis with Double Markers. In: Toyooka K, ed. <i>Neuronal
    Morphogenesis</i>. Vol 2831. 1st ed. MIMB. New York, NY: Springer Nature; 2024:283-299.
    doi:<a href="https://doi.org/10.1007/978-1-0716-3969-6_19">10.1007/978-1-0716-3969-6_19</a>'
  apa: 'Miranda, O., Cheung, G. T., &#38; Hippenmeyer, S. (2024). Morphological Analysis
    of Neurons and Glia Using Mosaic Analysis with Double Markers. In K. Toyooka (Ed.),
    <i>Neuronal Morphogenesis</i> (1st ed., Vol. 2831, pp. 283–299). New York, NY:
    Springer Nature. <a href="https://doi.org/10.1007/978-1-0716-3969-6_19">https://doi.org/10.1007/978-1-0716-3969-6_19</a>'
  chicago: 'Miranda, Osvaldo, Giselle T Cheung, and Simon Hippenmeyer. “Morphological
    Analysis of Neurons and Glia Using Mosaic Analysis with Double Markers.” In <i>Neuronal
    Morphogenesis</i>, edited by Kazuhito Toyooka, 1st ed., 2831:283–99. MIMB. New
    York, NY: Springer Nature, 2024. <a href="https://doi.org/10.1007/978-1-0716-3969-6_19">https://doi.org/10.1007/978-1-0716-3969-6_19</a>.'
  ieee: 'O. Miranda, G. T. Cheung, and S. Hippenmeyer, “Morphological Analysis of
    Neurons and Glia Using Mosaic Analysis with Double Markers,” in <i>Neuronal Morphogenesis</i>,
    1st ed., vol. 2831, K. Toyooka, Ed. New York, NY: Springer Nature, 2024, pp. 283–299.'
  ista: 'Miranda O, Cheung GT, Hippenmeyer S. 2024.Morphological Analysis of Neurons
    and Glia Using Mosaic Analysis with Double Markers. In: Neuronal Morphogenesis.
    Methods in Molecular Biology, vol. 2831, 283–299.'
  mla: Miranda, Osvaldo, et al. “Morphological Analysis of Neurons and Glia Using
    Mosaic Analysis with Double Markers.” <i>Neuronal Morphogenesis</i>, edited by
    Kazuhito Toyooka, 1st ed., vol. 2831, Springer Nature, 2024, pp. 283–99, doi:<a
    href="https://doi.org/10.1007/978-1-0716-3969-6_19">10.1007/978-1-0716-3969-6_19</a>.
  short: O. Miranda, G.T. Cheung, S. Hippenmeyer, in:, K. Toyooka (Ed.), Neuronal
    Morphogenesis, 1st ed., Springer Nature, New York, NY, 2024, pp. 283–299.
corr_author: '1'
date_created: 2024-08-13T12:16:41Z
date_published: 2024-08-13T00:00:00Z
date_updated: 2026-04-07T12:32:35Z
day: '13'
department:
- _id: GradSch
- _id: SiHi
doi: 10.1007/978-1-0716-3969-6_19
edition: '1'
editor:
- first_name: Kazuhito
  full_name: Toyooka, Kazuhito
  last_name: Toyooka
external_id:
  pmid:
  - '39134857'
intvolume: '      2831'
language:
- iso: eng
month: '08'
oa_version: None
page: 283-299
place: New York, NY
pmid: 1
project:
- _id: 34c9fbcb-11ca-11ed-8bc3-98fa5658610d
  grant_number: '26253'
  name: Molecular Mechanisms Regulating Cortical Neural Stem Cell Lineage Progression
    and Astrocyte Development
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F7805
  name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
    Mechanisms of Neural Stem Cell Lineage Progression
publication: Neuronal Morphogenesis
publication_identifier:
  eisbn:
  - '9781071639696'
  eissn:
  - 1940-6029
  isbn:
  - '9781071639689'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  record:
  - id: '20212'
    relation: dissertation_contains
    status: public
scopus_import: '1'
series_title: MIMB
status: public
title: Morphological Analysis of Neurons and Glia Using Mosaic Analysis with Double
  Markers
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2831
year: '2024'
...
---
OA_place: publisher
OA_type: green
_id: '6995'
abstract:
- lang: eng
  text: Human brain organoids represent a powerful tool for the study of human neurological
    diseases particularly those that impact brain growth and structure. However, many
    neurological diseases lack obvious anatomical abnormalities, yet significantly
    impact neural network functions, raising the question of whether organoids possess
    sufficient neural network architecture and complexity to model these conditions.
    Here, we explore the network level functions of brain organoids using calcium
    sensor imaging and extracellular recording approaches that together reveal the
    existence of complex oscillatory network behaviors reminiscent of intact brain
    preparations. We further demonstrate strikingly abnormal epileptiform network
    activity in organoids derived from a Rett Syndrome patient despite only modest
    anatomical differences from isogenically matched controls, and rescue with an
    unconventional neuromodulatory drug Pifithrin-α. Together, these findings provide
    an essential foundation for the utilization of human brain organoids to study
    intact and disordered human brain network formation and illustrate their utility
    in therapeutic discovery.
acknowledgement: We thank S. Butler, T. Carmichael and members of the laboratory of
  B.G.N. for helpful discussions and comments on the manuscript; N. Vishlaghi and
  F. Turcios-Hernandez for technical assistance, and J. Lee, S.-K. Lee, H. Shinagawa
  and K. Yoshikawa for valuable reagents. We also thank the UCLA Eli and Edythe Broad
  Stem Cell Research Center (BSCRC) and Intellectual and Developmental Disabilities
  Research Center microscopy cores for access to imaging facilities. This work was
  supported by grants from the California Institute for Regenerative Medicine (CIRM)
  (DISC1-08819 to B.G.N.), the National Institute of Health (R01NS089817, R01DA051897
  and P50HD103557 to B.G.N.; K08NS119747 to R.A.S.; K99HD096105 to M.W.; R01MH123922,
  R01MH121521 and P50HD103557 to M.J.G.; R01GM099134 to K.P.; R01NS103788 to W.E.L.;
  R01NS088571 to J.M.P.; R01NS030549 and R01AG050474 to I.M.), and research awards
  from the UCLA Jonsson Comprehensive Cancer Center and BSCRC Ablon Scholars Program
  (to B.G.N.), the BSCRC Innovation Program (to B.G.N., K.P. and W.E.L.), the UCLA
  BSCRC Steffy Brain Aging Research Fund (to B.G.N. and W.E.L.) and the UCLA Clinical
  and Translational Science Institute (to B.G.N.), Paul Allen Family Foundation Frontiers
  Group (to K.P. and W.E.L.), the March of Dimes Foundation (to W.E.L.) and the Simons
  Foundation Autism Research Initiative Bridge to Independence Program (to R.A.S.
  and M.J.G.). R.A.S. was also supported by the UCLA/NINDS Translational Neuroscience
  Training Grant (R25NS065723), a Research and Training Fellowship from the American
  Epilepsy Society, a Taking Flight Award from CURE Epilepsy and a Clinician Scientist
  training award from the UCLA BSCRC. J.E.B. was supported by the UCLA BSCRC Rose
  Hills Foundation Graduate Scholarship Training Program. M.W. was supported by postdoctoral
  training awards provided by the UCLA BSCRC and the Uehara Memorial Foundation. O.A.M.
  and A.K. were supported in part by the UCLA-California State University Northridge
  CIRM-Bridges training program (EDUC2-08411). We also acknowledge the support of
  the IDDRC Cells, Circuits and Systems Analysis, Microscopy and Genetics and Genomics
  Cores of the Semel Institute of Neuroscience at UCLA, which are supported by the
  NICHD (U54HD087101 and P50HD10355701). We lastly acknowledge support from a Quantitative
  and Computational Biosciences Collaboratory Postdoctoral Fellowship to S.M. and
  the Quantitative and Computational Biosciences Collaboratory community, directed
  by M. Pellegrini.
article_processing_charge: No
article_type: review
author:
- first_name: Ranmal A.
  full_name: Samarasinghe, Ranmal A.
  last_name: Samarasinghe
- first_name: Osvaldo
  full_name: Miranda, Osvaldo
  id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
  last_name: Miranda
  orcid: 0000-0001-6618-6889
- first_name: Jessie E.
  full_name: Buth, Jessie E.
  last_name: Buth
- first_name: Simon
  full_name: Mitchell, Simon
  last_name: Mitchell
- first_name: Isabella
  full_name: Ferando, Isabella
  last_name: Ferando
- first_name: Momoko
  full_name: Watanabe, Momoko
  last_name: Watanabe
- first_name: Arinnae
  full_name: Kurdian, Arinnae
  last_name: Kurdian
- first_name: Peyman
  full_name: Golshani, Peyman
  last_name: Golshani
- first_name: Kathrin
  full_name: Plath, Kathrin
  last_name: Plath
- first_name: William E.
  full_name: Lowry, William E.
  last_name: Lowry
- first_name: Jack M.
  full_name: Parent, Jack M.
  last_name: Parent
- first_name: Istvan
  full_name: Mody, Istvan
  last_name: Mody
- first_name: Bennett G.
  full_name: Novitch, Bennett G.
  last_name: Novitch
citation:
  ama: Samarasinghe RA, Miranda O, Buth JE, et al. Identification of neural oscillations
    and epileptiform changes in human brain organoids. <i>Nature Neuroscience</i>.
    2021;24:32. doi:<a href="https://doi.org/10.1038/s41593-021-00906-5">10.1038/s41593-021-00906-5</a>
  apa: Samarasinghe, R. A., Miranda, O., Buth, J. E., Mitchell, S., Ferando, I., Watanabe,
    M., … Novitch, B. G. (2021). Identification of neural oscillations and epileptiform
    changes in human brain organoids. <i>Nature Neuroscience</i>. Springer Nature.
    <a href="https://doi.org/10.1038/s41593-021-00906-5">https://doi.org/10.1038/s41593-021-00906-5</a>
  chicago: Samarasinghe, Ranmal A., Osvaldo Miranda, Jessie E. Buth, Simon Mitchell,
    Isabella Ferando, Momoko Watanabe, Arinnae Kurdian, et al. “Identification of
    Neural Oscillations and Epileptiform Changes in Human Brain Organoids.” <i>Nature
    Neuroscience</i>. Springer Nature, 2021. <a href="https://doi.org/10.1038/s41593-021-00906-5">https://doi.org/10.1038/s41593-021-00906-5</a>.
  ieee: R. A. Samarasinghe <i>et al.</i>, “Identification of neural oscillations and
    epileptiform changes in human brain organoids,” <i>Nature Neuroscience</i>, vol.
    24. Springer Nature, p. 32, 2021.
  ista: Samarasinghe RA, Miranda O, Buth JE, Mitchell S, Ferando I, Watanabe M, Kurdian
    A, Golshani P, Plath K, Lowry WE, Parent JM, Mody I, Novitch BG. 2021. Identification
    of neural oscillations and epileptiform changes in human brain organoids. Nature
    Neuroscience. 24, 32.
  mla: Samarasinghe, Ranmal A., et al. “Identification of Neural Oscillations and
    Epileptiform Changes in Human Brain Organoids.” <i>Nature Neuroscience</i>, vol.
    24, Springer Nature, 2021, p. 32, doi:<a href="https://doi.org/10.1038/s41593-021-00906-5">10.1038/s41593-021-00906-5</a>.
  short: R.A. Samarasinghe, O. Miranda, J.E. Buth, S. Mitchell, I. Ferando, M. Watanabe,
    A. Kurdian, P. Golshani, K. Plath, W.E. Lowry, J.M. Parent, I. Mody, B.G. Novitch,
    Nature Neuroscience 24 (2021) 32.
date_created: 2019-11-10T11:23:58Z
date_published: 2021-08-23T00:00:00Z
date_updated: 2025-07-09T09:00:12Z
day: '23'
department:
- _id: GradSch
- _id: SiHi
doi: 10.1038/s41593-021-00906-5
external_id:
  isi:
  - '000687516300001'
  pmid:
  - '34426698 '
intvolume: '        24'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/820183
month: '08'
oa: 1
oa_version: Preprint
page: '32'
pmid: 1
publication: Nature Neuroscience
publication_identifier:
  eissn:
  - 1546-1726
  issn:
  - 1097-6256
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Identification of neural oscillations and epileptiform changes in human brain
  organoids
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2021'
...
---
_id: '7358'
abstract:
- lang: eng
  text: Telencephalic organoids generated from human pluripotent stem cells (hPSCs)
    are emerging as an effective system to study the distinct features of the developing
    human brain and the underlying causes of many neurological disorders. While progress
    in organoid technology has been steadily advancing, many challenges remain including
    rampant batch-to-batch and cell line-to-cell line variability and irreproducibility.
    Here, we demonstrate that a major contributor to successful cortical organoid
    production is the manner in which hPSCs are maintained prior to differentiation.
    Optimal results were achieved using fibroblast-feeder-supported hPSCs compared
    to feeder-independent cells, related to differences in their transcriptomic states.
    Feeder-supported hPSCs display elevated activation of diverse TGFβ superfamily
    signaling pathways and increased expression of genes associated with naïve pluripotency.
    We further identify combinations of TGFβ-related growth factors that are necessary
    and together sufficient to impart broad telencephalic organoid competency to feeder-free
    hPSCs and enable reproducible formation of brain structures suitable for disease
    modeling.
article_processing_charge: No
author:
- first_name: Momoko
  full_name: Watanabe, Momoko
  last_name: Watanabe
- first_name: Jillian R.
  full_name: Haney, Jillian R.
  last_name: Haney
- first_name: Neda
  full_name: Vishlaghi, Neda
  last_name: Vishlaghi
- first_name: Felix
  full_name: Turcios, Felix
  last_name: Turcios
- first_name: Jessie E.
  full_name: Buth, Jessie E.
  last_name: Buth
- first_name: Wen
  full_name: Gu, Wen
  last_name: Gu
- first_name: Amanda J.
  full_name: Collier, Amanda J.
  last_name: Collier
- first_name: Osvaldo
  full_name: Miranda, Osvaldo
  id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
  last_name: Miranda
  orcid: 0000-0001-6618-6889
- first_name: Di
  full_name: Chen, Di
  last_name: Chen
- first_name: Shan
  full_name: Sabri, Shan
  last_name: Sabri
- first_name: Amander T.
  full_name: Clark, Amander T.
  last_name: Clark
- first_name: Kathrin
  full_name: Plath, Kathrin
  last_name: Plath
- first_name: Heather R.
  full_name: Christofk, Heather R.
  last_name: Christofk
- first_name: Michael J.
  full_name: Gandal, Michael J.
  last_name: Gandal
- first_name: Bennett G.
  full_name: Novitch, Bennett G.
  last_name: Novitch
citation:
  ama: Watanabe M, Haney JR, Vishlaghi N, et al. TGFβ superfamily signaling regulates
    the state of human stem cell pluripotency and competency to create telencephalic
    organoids. <i>bioRxiv</i>. 2019. doi:<a href="https://doi.org/10.1101/2019.12.13.875773">10.1101/2019.12.13.875773</a>
  apa: Watanabe, M., Haney, J. R., Vishlaghi, N., Turcios, F., Buth, J. E., Gu, W.,
    … Novitch, B. G. (2019). TGFβ superfamily signaling regulates the state of human
    stem cell pluripotency and competency to create telencephalic organoids. <i>bioRxiv</i>.
    Cold Spring Harbor Laboratory. <a href="https://doi.org/10.1101/2019.12.13.875773">https://doi.org/10.1101/2019.12.13.875773</a>
  chicago: Watanabe, Momoko, Jillian R. Haney, Neda Vishlaghi, Felix Turcios, Jessie
    E. Buth, Wen Gu, Amanda J. Collier, et al. “TGFβ Superfamily Signaling Regulates
    the State of Human Stem Cell Pluripotency and Competency to Create Telencephalic
    Organoids.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, 2019. <a href="https://doi.org/10.1101/2019.12.13.875773">https://doi.org/10.1101/2019.12.13.875773</a>.
  ieee: M. Watanabe <i>et al.</i>, “TGFβ superfamily signaling regulates the state
    of human stem cell pluripotency and competency to create telencephalic organoids,”
    <i>bioRxiv</i>. Cold Spring Harbor Laboratory, 2019.
  ista: Watanabe M, Haney JR, Vishlaghi N, Turcios F, Buth JE, Gu W, Collier AJ, Miranda
    O, Chen D, Sabri S, Clark AT, Plath K, Christofk HR, Gandal MJ, Novitch BG. 2019.
    TGFβ superfamily signaling regulates the state of human stem cell pluripotency
    and competency to create telencephalic organoids. bioRxiv, <a href="https://doi.org/10.1101/2019.12.13.875773">10.1101/2019.12.13.875773</a>.
  mla: Watanabe, Momoko, et al. “TGFβ Superfamily Signaling Regulates the State of
    Human Stem Cell Pluripotency and Competency to Create Telencephalic Organoids.”
    <i>BioRxiv</i>, Cold Spring Harbor Laboratory, 2019, doi:<a href="https://doi.org/10.1101/2019.12.13.875773">10.1101/2019.12.13.875773</a>.
  short: M. Watanabe, J.R. Haney, N. Vishlaghi, F. Turcios, J.E. Buth, W. Gu, A.J.
    Collier, O. Miranda, D. Chen, S. Sabri, A.T. Clark, K. Plath, H.R. Christofk,
    M.J. Gandal, B.G. Novitch, BioRxiv (2019).
date_created: 2020-01-23T09:53:40Z
date_published: 2019-12-13T00:00:00Z
date_updated: 2022-06-17T08:03:32Z
day: '13'
doi: 10.1101/2019.12.13.875773
extern: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/2019.12.13.875773
month: '12'
oa: 1
oa_version: Preprint
page: '75'
publication: bioRxiv
publication_status: published
publisher: Cold Spring Harbor Laboratory
status: public
title: TGFβ superfamily signaling regulates the state of human stem cell pluripotency
  and competency to create telencephalic organoids
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2019'
...