[{"pmid":1,"author":[{"full_name":"Vignolle, Anna","last_name":"Vignolle","first_name":"Anna"},{"first_name":"Martin","last_name":"Zehl","orcid":"0000-0001-9685-0373","full_name":"Zehl, Martin","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55"},{"last_name":"Garzón","full_name":"Garzón, Jaime Felipe Guerrero","first_name":"Jaime Felipe Guerrero"},{"full_name":"Schneider, Olha","last_name":"Schneider","first_name":"Olha"},{"first_name":"Johannes","full_name":"Gafriller, Johannes","last_name":"Gafriller"},{"full_name":"Grienke, Ulrike","last_name":"Grienke","first_name":"Ulrike"},{"first_name":"Rasmus H.","last_name":"Kirkegaard","full_name":"Kirkegaard, Rasmus H."},{"first_name":"Sergey B.","full_name":"Zotchev, Sergey B.","last_name":"Zotchev"}],"has_accepted_license":"1","article_processing_charge":"Yes","external_id":{"pmid":["42036976"]},"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":19,"date_published":"2026-04-01T00:00:00Z","OA_place":"publisher","date_updated":"2026-05-07T08:22:41Z","publisher":"Wiley","citation":{"short":"A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke, R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026).","ista":"Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial Biotechnology. 19(4), e70357.","apa":"Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke, U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial Biotechnology</i>. Wiley. <a href=\"https://doi.org/10.1111/1751-7915.70357\">https://doi.org/10.1111/1751-7915.70357</a>","mla":"Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>, vol. 19, no. 4, e70357, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/1751-7915.70357\">10.1111/1751-7915.70357</a>.","chicago":"Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider, Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev. “Identification and Characterisation of the Gene Cluster Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/1751-7915.70357\">https://doi.org/10.1111/1751-7915.70357</a>.","ieee":"A. Vignolle <i>et al.</i>, “Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,” <i>Microbial Biotechnology</i>, vol. 19, no. 4. Wiley, 2026.","ama":"Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial Biotechnology</i>. 2026;19(4). doi:<a href=\"https://doi.org/10.1111/1751-7915.70357\">10.1111/1751-7915.70357</a>"},"department":[{"_id":"MassSpec"}],"publication_identifier":{"eissn":["1751-7915"]},"status":"public","quality_controlled":"1","scopus_import":"1","OA_type":"gold","oa_version":"Published Version","issue":"4","day":"01","month":"04","ddc":["570"],"doi":"10.1111/1751-7915.70357","intvolume":"        19","_id":"21779","language":[{"iso":"eng"}],"date_created":"2026-05-03T22:01:37Z","DOAJ_listed":"1","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2026-05-07T08:21:06Z","license":"https://creativecommons.org/licenses/by/4.0/","acknowledgement":"This work was supported by the University of Vienna. The authors thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR Centre (both of the Faculty of Chemistry, University of Vienna and members of the Vienna Life Science Instruments) for assistance with data acquisition. Open Access funding provided by Universitat Wien. This work was supported by Universität Wien.","type":"journal_article","publication":"Microbial Biotechnology","abstract":[{"text":"Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action, targeting the biotin biosynthesis pathway. Despite being highly active against mycobacteria in vitro, its development as an anti-tubercular agent has been hindered due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis may yield new analogues with improved pharmacological properties. Here, we describe the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary metabolites targeting a different enzyme in the biotin biosynthesis pathway, and two genes for streptavidins, proteins that strongly bind and sequester biotin. The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous expression, which suggested that the fatty acid required for the formation of acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in the acidomycin BGC resulted in a significant decrease in its yield but did not abrogate the biosynthesis completely.","lang":"eng"}],"article_number":"e70357","article_type":"original","publication_status":"published","oa":1,"file":[{"date_created":"2026-05-07T08:21:06Z","file_name":"2026_MicrobialBiotechnology_Vignolle.pdf","content_type":"application/pdf","checksum":"8c8aa660cef5394167e06f187adbabf0","success":1,"relation":"main_file","file_size":575492,"access_level":"open_access","date_updated":"2026-05-07T08:21:06Z","creator":"dernst","file_id":"21835"}],"title":"Identification and characterisation of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin"},{"month":"04","day":"20","oa_version":"Published Version","OA_type":"gold","quality_controlled":"1","status":"public","publication_identifier":{"issn":["1664-302X"]},"department":[{"_id":"MassSpec"}],"date_updated":"2026-06-10T07:49:04Z","publisher":"Frontiers Media","citation":{"ieee":"I. Tocino-Márquez <i>et al.</i>, “Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments,” <i>Frontiers in Microbiology</i>, vol. 17. Frontiers Media, 2026.","chicago":"Tocino-Márquez, Inmaculada, Martin Zehl, Jovana Batajic, Joana Séneca, Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N. Sekurova, and Sergey B. Zotchev. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” <i>Frontiers in Microbiology</i>. Frontiers Media, 2026. <a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">https://doi.org/10.3389/fmicb.2026.1793713</a>.","mla":"Tocino-Márquez, Inmaculada, et al. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp. from Freshwater Sediments.” <i>Frontiers in Microbiology</i>, vol. 17, 1793713, Frontiers Media, 2026, doi:<a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">10.3389/fmicb.2026.1793713</a>.","ama":"Tocino-Márquez I, Zehl M, Batajic J, et al. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. <i>Frontiers in Microbiology</i>. 2026;17. doi:<a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">10.3389/fmicb.2026.1793713</a>","short":"I. Tocino-Márquez, M. Zehl, J. Batajic, J. Séneca, P. Pjevac, J. Murillo-Alba, J. Martín, O.N. Sekurova, S.B. Zotchev, Frontiers in Microbiology 17 (2026).","apa":"Tocino-Márquez, I., Zehl, M., Batajic, J., Séneca, J., Pjevac, P., Murillo-Alba, J., … Zotchev, S. B. (2026). Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. <i>Frontiers in Microbiology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fmicb.2026.1793713\">https://doi.org/10.3389/fmicb.2026.1793713</a>","ista":"Tocino-Márquez I, Zehl M, Batajic J, Séneca J, Pjevac P, Murillo-Alba J, Martín J, Sekurova ON, Zotchev SB. 2026. Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 17, 1793713."},"OA_place":"publisher","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":17,"date_published":"2026-04-20T00:00:00Z","external_id":{"pmid":["42088272"]},"article_processing_charge":"Yes","has_accepted_license":"1","pmid":1,"author":[{"full_name":"Tocino-Márquez, Inmaculada","last_name":"Tocino-Márquez","first_name":"Inmaculada"},{"orcid":"0000-0001-9685-0373","last_name":"Zehl","id":"8e016d5b-5d77-11f0-86d2-96cdb3922a55","full_name":"Zehl, Martin","first_name":"Martin"},{"first_name":"Jovana","last_name":"Batajic","full_name":"Batajic, Jovana"},{"last_name":"Séneca","full_name":"Séneca, Joana","first_name":"Joana"},{"last_name":"Pjevac","full_name":"Pjevac, Petra","first_name":"Petra"},{"last_name":"Murillo-Alba","full_name":"Murillo-Alba, José","first_name":"José"},{"last_name":"Martín","full_name":"Martín, Jesús","first_name":"Jesús"},{"first_name":"Olga N.","full_name":"Sekurova, Olga N.","last_name":"Sekurova"},{"first_name":"Sergey B.","full_name":"Zotchev, Sergey B.","last_name":"Zotchev"}],"title":"Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater sediments","file":[{"date_created":"2026-06-10T07:46:30Z","content_type":"application/pdf","checksum":"31fb6b98c8a6d4007cb21808c6d2d9e3","file_name":"2026_FrontiersMicrobiology_TocinoMarquez.pdf","file_size":3582644,"access_level":"open_access","relation":"main_file","success":1,"file_id":"21989","date_updated":"2026-06-10T07:46:30Z","creator":"dernst"}],"publication_status":"published","article_type":"original","oa":1,"article_number":"1793713","abstract":[{"lang":"eng","text":"Several Streptomyces strains were isolated from freshwater sediments collected in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses revealed biosynthetic gene clusters (BGCs) that may specify production of chemically diverse secondary metabolites. Various culture conditions were employed to induce metabolite production, and subsequent LC-MS analyses facilitated the identification of the produced compounds and their correlation with the corresponding BGCs. These analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic potential, revealing a diverse range of bioactive secondary metabolites, including siderophores, antibiotics, and other compounds with potential therapeutic applications. Genomes of two Streptomyces isolates, one of them representing a potentially new species, harbored several uncharacterized BGCs that may specify biosynthesis of novel secondary metabolites. Although targeted overexpression of pathway-specific regulators from these BGCs did not yield additional metabolites, whereas knockout experiments led to metabolic changes, presumably reflecting regulatory or compensatory interactions between multiple biosynthetic pathways. Continued exploration of these strains and their BGCs may lead to the discovery of new bioactive molecules with pharmaceutical and biotechnological applications."}],"publication":"Frontiers in Microbiology","acknowledgement":"The computational results of this work have been achieved using the Life Science Compute Cluster (LiSC) of the University of Vienna. We additionally thank Julia Ramesmayer for assistance during DNA extraction and sample preparation for long-read sequencing. Support from the Mass Spectrometry Centre of the Faculty of Chemistry, University of Vienna, is thankfully acknowledged.\r\nThe author(s) declared that financial support was received for this work and/or its publication. This work was supported by the University of Vienna via the Research Platform Secondary Metabolomes of Bacterial Communities (MetaBac). Open access funding provided by University of Vienna. ","type":"journal_article","file_date_updated":"2026-06-10T07:46:30Z","PlanS_conform":"1","date_created":"2026-06-08T08:34:10Z","DOAJ_listed":"1","language":[{"iso":"eng"}],"year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"21953","intvolume":"        17","doi":"10.3389/fmicb.2026.1793713","ddc":["572"]}]