[{"month":"01","OA_place":"publisher","acknowledgement":"This work was supported by the Basic Science Center Project of National Natural Science Foundation of China (32388201) to K.C and the National Natural Science Foundation of China (31970331) to L.X. We thank Dr. Zhuang Lu, Dr. Bin Han and Ms. Jingquan Li (Plant Science Facility of the Institute of Botany, Chinese Academy of Sciences) for their technical assistance in LC-MS/MS assay, small molecule compound analysis and the subcellular localization assay, respectively. We thank Dr. Wei Luo and Dr. Dongfeng Liu for helpful discussions.","_id":"21158","publication_status":"published","pmid":1,"scopus_import":"1","doi":"10.1038/s41467-025-67734-0","abstract":[{"text":"Vernalization-regulated flowering is vital for wheat yield and geographical distribution, and the diversity of flowering time genes is essential for the breeding of climate-resilient varieties. Sugars have long been recognized in regulating flowering; however, the intrinsic connection between carbohydrate metabolism and vernalization response remains largely unexplored. Here, we identify a fructose 1,6-bisphosphate aldolase (FBA) encoding gene, HtL1/FBA10, as a modulator of heading time variation based on a genome-wide association study utilizing wheat core germplasm collections. Evolutionary analysis shows a decrease in the proportion of haplotype-2 of HtL1, which is linked to delayed flowering, in Chinese and American wheat varieties compared to landraces. Vernalization reduces HtL1/FBA10 phosphorylation levels and  increases  its O-GlcNAcylation, which in turn enhances its enzymatic activity and facilitates VERNALIZATION 1 (VRN1) transcription by regulating histone acetylation at the VRN1 locus. Our findings provide mechanistic insights into the interplay between glucose metabolism and the epigenetic regulation of vernalization in winter wheat.","lang":"eng"}],"PlanS_conform":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":17,"title":"O-GlcNAc and phosphorylation modifications on HtL1/FBA10 regulate wheat vernalization for flowering","publication":"Nature Communications","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"file":[{"access_level":"open_access","file_size":4685882,"file_name":"2026_NatureComm_Yang.pdf","checksum":"9ae170ec70ba1ab56b6f1ffe67d1de7f","date_created":"2026-02-12T14:33:14Z","relation":"main_file","date_updated":"2026-02-12T14:33:14Z","content_type":"application/pdf","creator":"dernst","success":1,"file_id":"21223"}],"oa":1,"oa_version":"Published Version","ddc":["580"],"year":"2026","has_accepted_license":"1","intvolume":"        17","OA_type":"gold","type":"journal_article","language":[{"iso":"eng"}],"file_date_updated":"2026-02-12T14:33:14Z","DOAJ_listed":"1","quality_controlled":"1","date_published":"2026-01-27T00:00:00Z","date_updated":"2026-02-12T14:34:24Z","citation":{"apa":"Yang, P., Liu, Y., Dong, Q., Miao, Y., Zhang, J., Xu, S., … Chong, K. (2026). O-GlcNAc and phosphorylation modifications on HtL1/FBA10 regulate wheat vernalization for flowering. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-67734-0\">https://doi.org/10.1038/s41467-025-67734-0</a>","chicago":"Yang, Pengfang, Yangyang Liu, Qi Dong, Yuting Miao, Jianlong Zhang, Shujuan Xu, Hong Zhao, et al. “O-GlcNAc and Phosphorylation Modifications on HtL1/FBA10 Regulate Wheat Vernalization for Flowering.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-025-67734-0\">https://doi.org/10.1038/s41467-025-67734-0</a>.","short":"P. Yang, Y. Liu, Q. Dong, Y. Miao, J. Zhang, S. Xu, H. Zhao, Y. Niu, X. Zhang, Y. Xu, Z. Guo, L. Xing, K. Chong, Nature Communications 17 (2026).","mla":"Yang, Pengfang, et al. “O-GlcNAc and Phosphorylation Modifications on HtL1/FBA10 Regulate Wheat Vernalization for Flowering.” <i>Nature Communications</i>, vol. 17, 999, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-025-67734-0\">10.1038/s41467-025-67734-0</a>.","ama":"Yang P, Liu Y, Dong Q, et al. O-GlcNAc and phosphorylation modifications on HtL1/FBA10 regulate wheat vernalization for flowering. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-025-67734-0\">10.1038/s41467-025-67734-0</a>","ista":"Yang P, Liu Y, Dong Q, Miao Y, Zhang J, Xu S, Zhao H, Niu Y, Zhang X, Xu Y, Guo Z, Xing L, Chong K. 2026. O-GlcNAc and phosphorylation modifications on HtL1/FBA10 regulate wheat vernalization for flowering. Nature Communications. 17, 999.","ieee":"P. Yang <i>et al.</i>, “O-GlcNAc and phosphorylation modifications on HtL1/FBA10 regulate wheat vernalization for flowering,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026."},"date_created":"2026-02-08T23:02:48Z","department":[{"_id":"XiFe"}],"day":"27","article_number":"999","status":"public","publication_identifier":{"eissn":["2041-1723"]},"article_processing_charge":"Yes","external_id":{"pmid":["41455723"]},"publisher":"Springer Nature","article_type":"original","author":[{"first_name":"Pengfang","last_name":"Yang","full_name":"Yang, Pengfang"},{"full_name":"Liu, Yangyang","first_name":"Yangyang","last_name":"Liu"},{"full_name":"Dong, Qi","last_name":"Dong","first_name":"Qi"},{"last_name":"Miao","first_name":"Yuting","full_name":"Miao, Yuting"},{"last_name":"Zhang","first_name":"Jianlong","full_name":"Zhang, Jianlong"},{"full_name":"Xu, Shujuan","first_name":"Shujuan","id":"9724dd9d-f591-11ee-bd51-e97ed0652286","last_name":"Xu"},{"full_name":"Zhao, Hong","first_name":"Hong","last_name":"Zhao"},{"last_name":"Niu","first_name":"Yuda","full_name":"Niu, Yuda"},{"full_name":"Zhang, Xueyong","first_name":"Xueyong","last_name":"Zhang"},{"full_name":"Xu, Yunyuan","last_name":"Xu","first_name":"Yunyuan"},{"full_name":"Guo, Zifeng","first_name":"Zifeng","last_name":"Guo"},{"last_name":"Xing","first_name":"Lijing","full_name":"Xing, Lijing"},{"full_name":"Chong, Kang","first_name":"Kang","last_name":"Chong"}]},{"quality_controlled":"1","file_date_updated":"2025-12-29T13:40:32Z","date_published":"2025-05-29T00:00:00Z","citation":{"short":"J. Walker, J. Zhang, Y. Liu, S. Xu, Y. Yu, M. Vickers, W. Ouyang, J. Tálas, L. Dolan, K. Nakajima, X. Feng, Cell 188 (2025) 2890–2906.e14.","mla":"Walker, James, et al. “Extensive N4 Cytosine Methylation Is Essential for Marchantia Sperm Function.” <i>Cell</i>, vol. 188, no. 11, Elsevier, 2025, p. 2890–2906.e14, doi:<a href=\"https://doi.org/10.1016/j.cell.2025.03.014\">10.1016/j.cell.2025.03.014</a>.","apa":"Walker, J., Zhang, J., Liu, Y., Xu, S., Yu, Y., Vickers, M., … Feng, X. (2025). Extensive N4 cytosine methylation is essential for Marchantia sperm function. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2025.03.014\">https://doi.org/10.1016/j.cell.2025.03.014</a>","chicago":"Walker, James, Jingyi Zhang, Yalin Liu, Shujuan Xu, Yiming Yu, Martin Vickers, Weizhi Ouyang, et al. “Extensive N4 Cytosine Methylation Is Essential for Marchantia Sperm Function.” <i>Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.cell.2025.03.014\">https://doi.org/10.1016/j.cell.2025.03.014</a>.","ieee":"J. Walker <i>et al.</i>, “Extensive N4 cytosine methylation is essential for Marchantia sperm function,” <i>Cell</i>, vol. 188, no. 11. Elsevier, p. 2890–2906.e14, 2025.","ista":"Walker J, Zhang J, Liu Y, Xu S, Yu Y, Vickers M, Ouyang W, Tálas J, Dolan L, Nakajima K, Feng X. 2025. Extensive N4 cytosine methylation is essential for Marchantia sperm function. Cell. 188(11), 2890–2906.e14.","ama":"Walker J, Zhang J, Liu Y, et al. Extensive N4 cytosine methylation is essential for Marchantia sperm function. <i>Cell</i>. 2025;188(11):2890-2906.e14. doi:<a href=\"https://doi.org/10.1016/j.cell.2025.03.014\">10.1016/j.cell.2025.03.014</a>"},"date_updated":"2026-04-28T13:36:51Z","intvolume":"       188","OA_type":"hybrid","project":[{"call_identifier":"H2020","name":"Establishment, modulation and inheritance of sexual lineage specific DNA methylation in plants","grant_number":"804981","_id":"bdb51a6e-d553-11ed-ba76-c2025f3d5725"}],"type":"journal_article","language":[{"iso":"eng"}],"article_type":"original","publisher":"Elsevier","external_id":{"pmid":["40209706"],"isi":["001504744800006"]},"author":[{"full_name":"Walker, James","last_name":"Walker","first_name":"James"},{"last_name":"Zhang","first_name":"Jingyi","full_name":"Zhang, Jingyi"},{"last_name":"Liu","first_name":"Yalin","full_name":"Liu, Yalin"},{"full_name":"Xu, Shujuan","last_name":"Xu","id":"9724dd9d-f591-11ee-bd51-e97ed0652286","first_name":"Shujuan"},{"full_name":"Yu, Yiming","orcid":"0000-0002-9919-7282","last_name":"Yu","id":"318e643b-8b61-11ed-b69e-aafa103ec8dd","first_name":"Yiming"},{"last_name":"Vickers","first_name":"Martin","full_name":"Vickers, Martin"},{"first_name":"Weizhi","last_name":"Ouyang","id":"fec73395-8b60-11ed-b69e-927fda99c743","full_name":"Ouyang, Weizhi"},{"first_name":"Judit","last_name":"Tálas","full_name":"Tálas, Judit"},{"first_name":"Liam","last_name":"Dolan","full_name":"Dolan, Liam"},{"last_name":"Nakajima","first_name":"Keiji","full_name":"Nakajima, Keiji"},{"orcid":"0000-0002-4008-1234","full_name":"Feng, Xiaoqi","first_name":"Xiaoqi","last_name":"Feng","id":"e0164712-22ee-11ed-b12a-d80fcdf35958"}],"department":[{"_id":"XiFe"}],"date_created":"2025-04-20T22:01:28Z","corr_author":"1","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/from-bacterial-immunity-to-plant-sex/"}]},"day":"29","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]},"status":"public","article_processing_charge":"Yes (via OA deal)","publication_status":"published","pmid":1,"page":"2890-2906.e14","_id":"19602","issue":"11","doi":"10.1016/j.cell.2025.03.014","scopus_import":"1","PlanS_conform":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"}],"abstract":[{"lang":"eng","text":"N4-methylcytosine (4mC) is an important DNA modification in prokaryotes, but its relevance and even its presence in eukaryotes have been mysterious. Here we show that spermatogenesis in the liverwort Marchantia polymorpha involves two waves of extensive DNA methylation reprogramming. First, 5-methylcytosine (5mC) expands from transposons to the entire genome. Notably, the second wave installs 4mC throughout genic regions, covering over 50% of CG sites in sperm. 4mC requires a methyltransferase (MpDN4MT1a) that is specifically expressed during late spermiogenesis. Deletion of MpDN4MT1a alters the sperm transcriptome, causes sperm swimming and fertility defects, and impairs post-fertilization development. Our results reveal extensive 4mC in a eukaryote, identify a family of eukaryotic methyltransferases, and elucidate the biological functions of 4mC in reproductive development, thereby expanding the repertoire of functional eukaryotic DNA modifications."}],"OA_place":"publisher","month":"05","acknowledgement":"We thank Sir Richard Roberts (NEB) for the kind gift of anti-4mC antibodies. We are also grateful to the JIC Small Molecule Mass Spectrometry (Lionel Hill) and Chemistry (Martin Rejzek) platforms as well as the High Resolution Metabolomics Laboratory (Manfred Beckmann, Aberystwyth University) for their assistance with LC-MS. Additionally, we acknowledge the assistance of the JIC Bioimaging Facility and ISTA Imaging and Optics Facility for microscopy. Finally, we appreciate the High Performance Computing resources provided by the ISTA Scientific Computing Facility and Norwich BioScience Institute Partnership Computing Infrastructure. This work was funded by a Sainsbury Charitable Foundation studentship (J.W.), a UKRI-BBSRC Doctoral Training Partnerships studentship (BBT0087171 to J.T.), a European Research Council Starting Grant (“SexMeth” 804981 to J.W., S.X., and X.F.), two Biotechnology and Biological Sciences Research Council (BBSRC) grants (BBS0096201 and BBP0135111 to J.Z., M.V., and X.F.), an EMBO Long Term Fellowship (Y.L.), an ISTA Bridge Fellowship (S.X.), and ISTA core funding (Y.Y. and X.F.).","isi":1,"file":[{"content_type":"application/pdf","creator":"dernst","success":1,"file_id":"20871","relation":"main_file","date_updated":"2025-12-29T13:40:32Z","file_name":"2025_Cell_Walker.pdf","checksum":"0dcc2feb368dfe7c4890093366b2dacb","date_created":"2025-12-29T13:40:32Z","file_size":11622960,"access_level":"open_access"}],"oa_version":"Published Version","oa":1,"year":"2025","ddc":["570"],"has_accepted_license":"1","title":"Extensive N4 cytosine methylation is essential for Marchantia sperm function","volume":188,"ec_funded":1,"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication":"Cell"},{"year":"2024","oa":1,"oa_version":"Preprint","isi":1,"publication":"Science China Life Sciences","volume":67,"title":"Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat","abstract":[{"text":"Winter plants rely on vernalization, a crucial process for adapting to cold conditions and ensuring successful reproduction. However, understanding the role of histone modifications in guiding the vernalization process in winter wheat remains limited. In this study, we investigated the transcriptome and chromatin dynamics in the shoot apex throughout the life cycle of winter wheat in the field. Two core histone modifications, H3K27me3 and H3K36me3, exhibited opposite patterns on the key vernalization gene VERNALIZATION1 (VRN1), correlating with its induction during cold exposure. Moreover, the H3K36me3 level remained high at VRN1 after cold exposure, which may maintain its active state. Mutations in FERTILIZATION-INDEPENDENT ENDOSPERM (TaFIE) and SET DOMAIN GROUP 8/EARLY FLOWERING IN SHORT DAYS (TaSDG8/TaEFS), components of the writer complex for H3K27me3 and H3K36me3, respectively, affected flowering time. Intriguingly, VRN1 lost its high expression after the cold exposure memory in the absence of H3K36me3. During embryo development, VRN1 was silenced with the removal of active histone modifications in both winter and spring wheat, with selective restoration of H3K27me3 in winter wheat. The mutant of Tafie-cr-87, a component of H3K27me3 “writer” complex, did not influence the silence of VRN1 during embryo development, but rather attenuated the cold exposure requirement of winter wheat. Integrating gene expression with H3K27me3 and H3K36me3 patterns identified potential regulators of flowering. This study unveils distinct roles of H3K27me3 and H3K36me3 in controlling vernalization response, maintenance, and resetting in winter wheat.","lang":"eng"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2023.12.19.572364"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","scopus_import":"1","doi":"10.1007/s11427-024-2664-0","_id":"17285","publication_status":"published","page":"2251-2266","pmid":1,"acknowledgement":"We thank Prof. Kang Chong from Institute of Botany, the Chinese Academy of Science for valuable comments, Dr. Haoran Li for the help with western blot of H3K36me3 in Tasdg8-cr lines. This research was supported by National Natural Science Foundation (31970529), Beijing Natural Science Foundation Outstanding Youth Project (JQ23026), National Key Research and Development Program of China (2021YFD1201500), and the Major Basic Research Program of Shandong Natural Science Foundation (ZR2019ZD15).","month":"10","OA_place":"repository","author":[{"first_name":"Xuemei","last_name":"Liu","full_name":"Liu, Xuemei"},{"full_name":"Deng, Min","first_name":"Min","last_name":"Deng"},{"full_name":"Shi, Bingxin","last_name":"Shi","first_name":"Bingxin"},{"full_name":"Zhu, Kehui","first_name":"Kehui","last_name":"Zhu"},{"full_name":"Chen, Jinchao","last_name":"Chen","first_name":"Jinchao"},{"full_name":"Xu, Shujuan","last_name":"Xu","id":"9724dd9d-f591-11ee-bd51-e97ed0652286","first_name":"Shujuan"},{"last_name":"Bie","first_name":"Xiaomin","full_name":"Bie, Xiaomin"},{"full_name":"Zhang, Xiansheng","first_name":"Xiansheng","last_name":"Zhang"},{"last_name":"Lin","first_name":"Xuelei","full_name":"Lin, Xuelei"},{"first_name":"Jun","last_name":"Xiao","full_name":"Xiao, Jun"}],"external_id":{"isi":["001268807700002"],"pmid":["38987431"]},"publisher":"Springer Nature","article_type":"original","publication_identifier":{"issn":["1674-7305"],"eissn":["1869-1889"]},"status":"public","article_processing_charge":"No","day":"01","date_created":"2024-07-21T22:01:02Z","department":[{"_id":"XiFe"}],"date_updated":"2025-09-08T08:15:08Z","citation":{"ieee":"X. Liu <i>et al.</i>, “Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat,” <i>Science China Life Sciences</i>, vol. 67. Springer Nature, pp. 2251–2266, 2024.","ama":"Liu X, Deng M, Shi B, et al. Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat. <i>Science China Life Sciences</i>. 2024;67:2251-2266. doi:<a href=\"https://doi.org/10.1007/s11427-024-2664-0\">10.1007/s11427-024-2664-0</a>","ista":"Liu X, Deng M, Shi B, Zhu K, Chen J, Xu S, Bie X, Zhang X, Lin X, Xiao J. 2024. Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat. Science China Life Sciences. 67, 2251–2266.","apa":"Liu, X., Deng, M., Shi, B., Zhu, K., Chen, J., Xu, S., … Xiao, J. (2024). Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat. <i>Science China Life Sciences</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s11427-024-2664-0\">https://doi.org/10.1007/s11427-024-2664-0</a>","chicago":"Liu, Xuemei, Min Deng, Bingxin Shi, Kehui Zhu, Jinchao Chen, Shujuan Xu, Xiaomin Bie, Xiansheng Zhang, Xuelei Lin, and Jun Xiao. “Distinct Roles of H3K27me3 and H3K36me3 in Vernalization Response, Maintenance, and Resetting in Winter Wheat.” <i>Science China Life Sciences</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1007/s11427-024-2664-0\">https://doi.org/10.1007/s11427-024-2664-0</a>.","short":"X. Liu, M. Deng, B. Shi, K. Zhu, J. Chen, S. Xu, X. Bie, X. Zhang, X. Lin, J. Xiao, Science China Life Sciences 67 (2024) 2251–2266.","mla":"Liu, Xuemei, et al. “Distinct Roles of H3K27me3 and H3K36me3 in Vernalization Response, Maintenance, and Resetting in Winter Wheat.” <i>Science China Life Sciences</i>, vol. 67, Springer Nature, 2024, pp. 2251–66, doi:<a href=\"https://doi.org/10.1007/s11427-024-2664-0\">10.1007/s11427-024-2664-0</a>."},"date_published":"2024-10-01T00:00:00Z","quality_controlled":"1","language":[{"iso":"eng"}],"OA_type":"green","type":"journal_article","intvolume":"        67"}]