[{"oa_version":"Published Version","intvolume":"       640","pmid":1,"date_created":"2025-03-19T09:44:39Z","date_updated":"2026-04-28T13:42:45Z","page":"1011-1016","day":"24","publication_status":"published","_id":"19421","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","quality_controlled":"1","doi":"10.1038/s41586-025-08669-w","publisher":"Springer Nature","file_date_updated":"2025-08-05T12:29:35Z","has_accepted_license":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"19478"}],"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/updating-the-textbook/"}]},"project":[{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"article_type":"original","publication":"Nature","department":[{"_id":"JiFr"}],"oa":1,"PlanS_conform":"1","date_published":"2025-04-24T00:00:00Z","external_id":{"pmid":["40044868"],"isi":["001437493900001"]},"language":[{"iso":"eng"}],"volume":640,"abstract":[{"lang":"eng","text":"The phytohormone auxin (Aux) is a principal endogenous developmental signal in plants. It mediates transcriptional reprogramming by a well-established canonical signalling mechanism. TIR1/AFB auxin receptors are F-box subunits of an ubiquitin ligase complex; after auxin perception, they associate with Aux/IAA transcriptional repressors and ubiquitinate them for degradation, thus enabling the activation of auxin response factor (ARF) transcription factors1,2,3. Here we revise this paradigm by showing that without TIR1 adenylate cyclase (AC) activity4, auxin-induced degradation of Aux/IAAs is not sufficient to mediate the transcriptional auxin response. Abolishing the TIR1 AC activity does not affect auxin-induced degradation of Aux/IAAs but renders TIR1 non-functional in mediating transcriptional reprogramming and auxin-regulated development, including shoot, root, root hair growth and lateral root formation. Transgenic plants show that local cAMP production in the vicinity of the Aux/IAA–ARF complex by unrelated AC enzymes bypasses the need for auxin perception and is sufficient to induce ARF-mediated transcription. These discoveries revise the canonical model of auxin signalling and establish TIR1/AFB-produced cAMP as a second messenger essential for transcriptional reprograming."}],"year":"2025","article_processing_charge":"Yes (via OA deal)","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"isi":1,"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"corr_author":"1","OA_type":"hybrid","file":[{"access_level":"open_access","file_size":13549245,"file_id":"20132","file_name":"2025_Nature_Chen.pdf","relation":"main_file","content_type":"application/pdf","success":1,"creator":"dernst","date_updated":"2025-08-05T12:29:35Z","checksum":"f5f18081003e7a1b8e372ecb7da82e7d","date_created":"2025-08-05T12:29:35Z"}],"month":"04","citation":{"ista":"Chen H, Qi L, Zou M, Lu M, Kwiatkowski M, Pei Y, Jaworski K, Friml J. 2025. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. 640, 1011–1016.","ama":"Chen H, Qi L, Zou M, et al. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. <i>Nature</i>. 2025;640:1011-1016. doi:<a href=\"https://doi.org/10.1038/s41586-025-08669-w\">10.1038/s41586-025-08669-w</a>","chicago":"Chen, Huihuang, Linlin Qi, Minxia Zou, Mengting Lu, M Kwiatkowski, Yuanrong Pei, K Jaworski, and Jiří Friml. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-08669-w\">https://doi.org/10.1038/s41586-025-08669-w</a>.","short":"H. Chen, L. Qi, M. Zou, M. Lu, M. Kwiatkowski, Y. Pei, K. Jaworski, J. Friml, Nature 640 (2025) 1011–1016.","mla":"Chen, Huihuang, et al. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” <i>Nature</i>, vol. 640, Springer Nature, 2025, pp. 1011–16, doi:<a href=\"https://doi.org/10.1038/s41586-025-08669-w\">10.1038/s41586-025-08669-w</a>.","apa":"Chen, H., Qi, L., Zou, M., Lu, M., Kwiatkowski, M., Pei, Y., … Friml, J. (2025). TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-08669-w\">https://doi.org/10.1038/s41586-025-08669-w</a>","ieee":"H. Chen <i>et al.</i>, “TIR1-produced cAMP as a second messenger in transcriptional auxin signalling,” <i>Nature</i>, vol. 640. Springer Nature, pp. 1011–1016, 2025."},"license":"https://creativecommons.org/licenses/by/4.0/","title":"TIR1-produced cAMP as a second messenger in transcriptional auxin signalling","OA_place":"publisher","ddc":["580"],"type":"journal_article","acknowledgement":"We are grateful to J. Callis and H.-Q. Yang for sharing materials and to M. Estelle and S. Kepinski for inspiring discussions. This research was supported by the Laboratory Support Facility, the Plant Facility and the Imaging and Optics Facility of the Institute of Science and Technology Austria. This project has received funding from the European Research Council (101142681 CYNIPS) and Austrian Science Fund (P 37051-B). L.Q. was supported by the National Natural Science Foundation of China (grant no. 32470327). M.Z. was supported by the Interdisciplinary Project Committee of the Institute of Science and Technology Austria, and Y.P. was supported by an EMBO Postdoctoral Fellowship (ALTF 38-2023). Open access funding provided by Institute of Science and Technology (IST Austria).","status":"public","author":[{"last_name":"Chen","first_name":"Huihuang","full_name":"Chen, Huihuang","id":"83c96512-15b2-11ec-abd3-b7eede36184f"},{"last_name":"Qi","first_name":"Linlin","full_name":"Qi, Linlin","orcid":"0000-0001-5187-8401","id":"44B04502-A9ED-11E9-B6FC-583AE6697425"},{"last_name":"Zou","first_name":"Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia"},{"full_name":"Lu, Mengting","id":"a8198a14-1ffe-11ee-8b67-d2bdff9d9178","first_name":"Mengting","last_name":"Lu"},{"last_name":"Kwiatkowski","first_name":"M","full_name":"Kwiatkowski, M"},{"full_name":"Pei, Yuanrong","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","last_name":"Pei","first_name":"Yuanrong"},{"full_name":"Jaworski, K","last_name":"Jaworski","first_name":"K"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"}]},{"publication_status":"published","day":"19","quality_controlled":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"19736","publisher":"Springer Nature","doi":"10.1038/s41580-025-00851-2","oa_version":"None","pmid":1,"article_number":"e113018","date_created":"2025-05-25T22:16:57Z","date_updated":"2025-09-30T12:41:30Z","scopus_import":"1","abstract":[{"lang":"eng","text":"The phytohormone auxin is a major signal coordinating growth and development in plants. The variety of its effects arises from its ability to form local auxin maxima and gradients within tissues, generated through directional cell-to-cell transport and elaborate metabolic control. These auxin distribution patterns instruct cells in a context-dependent manner to undergo predefined developmental transitions. In this Review, we discuss advances in auxin action at the level of homeostasis and signalling. We highlight key insights into the structural basis of PIN-mediated intercellular auxin transport and explore two novel non-transcriptional auxin signalling mechanisms: one involving intracellular Ca2+ transients and another involving cell-surface auxin perception that mediates global, ultrafast phosphorylation. Furthermore, we examine emerging evidence indicating the involvement of cyclic adenosine monophosphate as a second messenger in the transcriptional auxin response. Together, these recent developments in auxin research have profoundly deepened our understanding of the complex and diverse activities of auxin in plant growth and development."}],"article_processing_charge":"No","year":"2025","publication_identifier":{"issn":["1471-0072"],"eissn":["1471-0080"]},"isi":1,"publication":"Nature Reviews Molecular Cell Biology","department":[{"_id":"JiFr"}],"article_type":"review","date_published":"2025-05-19T00:00:00Z","external_id":{"isi":["001490500500001"],"pmid":["40389696"]},"language":[{"iso":"eng"}],"title":"Mechanisms of auxin action in plant growth and development","type":"journal_article","status":"public","author":[{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"first_name":"Yuanrong","last_name":"Pei","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","full_name":"Pei, Yuanrong"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"}],"corr_author":"1","OA_type":"closed access","citation":{"ama":"Vanneste S, Pei Y, Friml J. Mechanisms of auxin action in plant growth and development. <i>Nature Reviews Molecular Cell Biology</i>. 2025. doi:<a href=\"https://doi.org/10.1038/s41580-025-00851-2\">10.1038/s41580-025-00851-2</a>","short":"S. Vanneste, Y. Pei, J. Friml, Nature Reviews Molecular Cell Biology (2025).","apa":"Vanneste, S., Pei, Y., &#38; Friml, J. (2025). Mechanisms of auxin action in plant growth and development. <i>Nature Reviews Molecular Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41580-025-00851-2\">https://doi.org/10.1038/s41580-025-00851-2</a>","chicago":"Vanneste, Steffen, Yuanrong Pei, and Jiří Friml. “Mechanisms of Auxin Action in Plant Growth and Development.” <i>Nature Reviews Molecular Cell Biology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41580-025-00851-2\">https://doi.org/10.1038/s41580-025-00851-2</a>.","ieee":"S. Vanneste, Y. Pei, and J. Friml, “Mechanisms of auxin action in plant growth and development,” <i>Nature Reviews Molecular Cell Biology</i>. Springer Nature, 2025.","mla":"Vanneste, Steffen, et al. “Mechanisms of Auxin Action in Plant Growth and Development.” <i>Nature Reviews Molecular Cell Biology</i>, e113018, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41580-025-00851-2\">10.1038/s41580-025-00851-2</a>.","ista":"Vanneste S, Pei Y, Friml J. 2025. Mechanisms of auxin action in plant growth and development. Nature Reviews Molecular Cell Biology., e113018."},"month":"05"}]