@article{20818,
  abstract     = {This study demonstrates that Marchantia non-canonical PINs are predominantly localized to the plasma membrane, with MpPINX and MpPINW exhibiting asymmetric distribution.
A newly identified miniW domain within the MpPINW hydrophilic loop governs subcellular trafficking and asymmetric PM localization of non-canonical PINs in Marchantia.},
  author       = {Tang, Han and Smoljan, Adrijana and Zou, Minxia and Zhang, Yuzhou and Lu, Kuan Ju and Friml, Jiří},
  issn         = {1365-3040},
  journal      = {Plant Cell and Environment},
  publisher    = {Wiley},
  title        = {{The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha}},
  doi          = {10.1111/pce.70295},
  year         = {2025},
}

@article{19423,
  abstract     = {Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.
Here, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.
The thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.
These findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.},
  author       = {Kurtović, K and Vosolsobě, S and Nedvěd, D and Müller, K and Dobrev, PI and Schmidt, V and Piszczek, P and Kuhn, A and Smoljan, Adrijana and Fisher, TJ and Weijers, D and Friml, Jiří and Bowman, JL and Petrášek, J},
  issn         = {1469-8137},
  journal      = {New Phytologist},
  number       = {3},
  pages        = {1066--1083},
  publisher    = {Wiley},
  title        = {{The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii}},
  doi          = {10.1111/nph.70019},
  volume       = {246},
  year         = {2025},
}

@article{20187,
  abstract     = {Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development.},
  author       = {Babic, David and Abualia, Rashed and Fiedler, Lukas and Qi, Linlin and Tellier, Frédérique and Smoljan, Adrijana and Rakusova, Hana and Valošek, Petr and Han, Huibin and Benková, Eva and Faure, Jean Denis and Friml, Jiří},
  issn         = {1365-313X},
  journal      = {Plant Journal},
  number       = {3},
  publisher    = {Wiley},
  title        = {{Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development}},
  doi          = {10.1111/tpj.70396},
  volume       = {123},
  year         = {2025},
}

@article{19728,
  abstract     = {Root system integrates multiple environmental cues, chiefly gravity and soil humidity, to anchor plants in soil and forage for water. While the mechanism of auxin-mediated root gravitropism is comparably well-understood, the root’s capability to grow toward moist soil for water uptake and drought avoidance, termed root hydrotropism, remains largely mysterious. Here, we provide key insights into the mechanism of hydrotropic growth and assign a role to the master regulator of hydrotropism, MIZU-KUSSEI 1 (MIZ1). We show that efficient hydrotropism requires the attenuation of antagonistically acting gravitropism, which is inhibited under drought conditions. Drought stress interferes with subcellular trafficking and the lateral mobility of PIN auxin transporters, which are polarly localized at the root cell plasma membranes. This leads to defects in PIN2 polarity and gravity-induced polarization of PIN3, ultimately inhibiting gravity-induced auxin redistribution and root bending. The miz1 mutant is defective in all these regulations, and in support of MIZ1’s action on PINs, pin mutations rescue the hydrotropic defects in the miz1 mutant. These observations identify a mechanism for how drought via MIZ1 attenuates gravitropism to promote root hydrotropism for efficient water foraging under drought conditions.},
  author       = {Zhang, Yuzhou and Bao, Zhulatai and Smoljan, Adrijana and Liu, Yifan and Wang, Huihui and Friml, Jiří},
  issn         = {1091-6490},
  journal      = {Proceedings of the National Academy of Sciences},
  number       = {20},
  publisher    = {National Academy of Sciences},
  title        = {{Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism}},
  doi          = {10.1073/pnas.2427315122},
  volume       = {122},
  year         = {2025},
}