[{"publication":"Journal of Integrative Plant Biology","status":"public","publication_status":"epub_ahead","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"oa_version":"Published Version","day":"10","date_updated":"2026-07-13T14:26:31Z","date_published":"2026-06-10T00:00:00Z","article_processing_charge":"Yes (via OA deal)","title":"Auxin response and PIN‐mediated transport in chlorophyte algae","ddc":["580"],"researchdata_availability":"no","abstract":[{"lang":"eng","text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage."}],"citation":{"apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>","ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. 2026. doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>, jipb. 70309, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>.","ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309.","chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>.","ieee":"A. Smoljan <i>et al.</i>, “Auxin response and PIN‐mediated transport in chlorophyte algae,” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026."},"pmid":1,"article_type":"original","project":[{"name":"Cyclic nucleotides as second messengers in plants","grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Wiley","oa":1,"publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"OA_place":"publisher","quality_controlled":"1","month":"06","type":"journal_article","external_id":{"pmid":["42271607"]},"license":"https://creativecommons.org/licenses/by/4.0/","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_number":"jipb.70309","year":"2026","das_tickbox":"0","OA_type":"hybrid","corr_author":"1","main_file_link":[{"url":"https://doi.org/10.1111/jipb.70309","open_access":"1"}],"language":[{"iso":"eng"}],"acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","scopus_import":"1","_id":"22301","has_accepted_license":"1","doi":"10.1111/jipb.70309","PlanS_conform":"1","author":[{"first_name":"Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","full_name":"Smoljan, Adrijana"},{"full_name":"Koutnik‐Abele, Sarah","last_name":"Koutnik‐Abele","first_name":"Sarah"},{"first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev"},{"first_name":"Petr","full_name":"Klíma, Petr","last_name":"Klíma"},{"last_name":"Bírošíková","full_name":"Bírošíková, Anita","first_name":"Anita"},{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","full_name":"Zhang, Yuzhou","first_name":"Yuzhou","orcid":"0000-0003-2627-6956"},{"first_name":"Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack"},{"first_name":"Maximilian","full_name":"Schuster, Maximilian","last_name":"Schuster","id":"37e65def-d415-11eb-ae59-a7b67be103db"},{"first_name":"Katarina","last_name":"Kurtović","full_name":"Kurtović, Katarina"},{"first_name":"Ulrich Z.","last_name":"Hammes","full_name":"Hammes, Ulrich Z."},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"supplementarymaterial":"yes","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"date_created":"2026-07-13T10:44:55Z"},{"das_tickbox":"1","year":"2026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_type":"closed access","language":[{"iso":"eng"}],"scopus_import":"1","acknowledgement":"The authors sincerely thank Dr. Shutang Tan for experimental support and Dr. Barbara Kloeckener Gruissem for critical reading and constructive advice on the manuscript. This study was supported by the European Research Council Advanced Grant (ETAP-742985 to H.T. and J.F.), by the Ministry of Science and Technology (grant 112-2636-B-005-001- to K.-J.L.), and by the Ministry of Education (grant MOE-109-YSFAG-0006-001-P1 to K.-J.L.).","issue":"3","doi":"10.1111/pce.70295","_id":"20818","date_created":"2025-12-14T23:02:05Z","department":[{"_id":"JiFr"}],"supplementarymaterial":"yes","author":[{"orcid":"0000-0001-6152-6637","first_name":"Han","full_name":"Tang, Han","id":"19BDF720-25A0-11EA-AC6E-928F3DDC885E","last_name":"Tang"},{"full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan","first_name":"Adrijana"},{"full_name":"Zou, Minxia","last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia"},{"full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","orcid":"0000-0003-2627-6956","first_name":"Yuzhou"},{"first_name":"Kuan Ju","last_name":"Lu","full_name":"Lu, Kuan Ju"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"publication_status":"published","status":"public","publication":"Plant Cell and Environment","article_processing_charge":"No","date_published":"2026-03-01T00:00:00Z","date_updated":"2026-07-27T10:27:47Z","day":"01","oa_version":"None","researchdata_availability":"upon request","title":"The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha","abstract":[{"lang":"eng","text":"This study demonstrates that Marchantia non-canonical PINs are predominantly localized to the plasma membrane, with MpPINX and MpPINW exhibiting asymmetric distribution.\r\nA newly identified miniW domain within the MpPINW hydrophilic loop governs subcellular trafficking and asymmetric PM localization of non-canonical PINs in Marchantia."}],"dataavailabilitystatement":"The data that support the findings of this study are available from the corresponding author upon reasonable request.","ec_funded":1,"citation":{"mla":"Tang, Han, et al. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” <i>Plant Cell and Environment</i>, vol. 49, no. 3, Wiley, 2026, pp. 1505–08, doi:<a href=\"https://doi.org/10.1111/pce.70295\">10.1111/pce.70295</a>.","short":"H. Tang, A. Smoljan, M. Zou, Y. Zhang, K.J. Lu, J. Friml, Plant Cell and Environment 49 (2026) 1505–1508.","ieee":"H. Tang, A. Smoljan, M. Zou, Y. Zhang, K. J. Lu, and J. Friml, “The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha,” <i>Plant Cell and Environment</i>, vol. 49, no. 3. Wiley, pp. 1505–1508, 2026.","ista":"Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. 2026. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. Plant Cell and Environment. 49(3), 1505–1508.","chicago":"Tang, Han, Adrijana Smoljan, Minxia Zou, Yuzhou Zhang, Kuan Ju Lu, and Jiří Friml. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical PINs in Marchantia Polymorpha.” <i>Plant Cell and Environment</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/pce.70295\">https://doi.org/10.1111/pce.70295</a>.","apa":"Tang, H., Smoljan, A., Zou, M., Zhang, Y., Lu, K. J., &#38; Friml, J. (2026). The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. <i>Plant Cell and Environment</i>. Wiley. <a href=\"https://doi.org/10.1111/pce.70295\">https://doi.org/10.1111/pce.70295</a>","ama":"Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. The miniW domain directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha. <i>Plant Cell and Environment</i>. 2026;49(3):1505-1508. doi:<a href=\"https://doi.org/10.1111/pce.70295\">10.1111/pce.70295</a>"},"pmid":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"article_type":"comment","intvolume":"        49","publication_identifier":{"issn":["0140-7791"],"eissn":["1365-3040"]},"publisher":"Wiley","volume":49,"type":"journal_article","external_id":{"pmid":["41340422"]},"month":"03","page":"1505-1508","quality_controlled":"1"},{"isi":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2025","OA_type":"hybrid","file_date_updated":"2025-04-16T08:03:36Z","language":[{"iso":"eng"}],"acknowledgement":"This work was supported by funding from the Czech Science Foundation project no. 20-13587S to JP and SV, Charles University Grant Agency projects no. 289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge the Imaging Facility of the Institute of Experimental Botany AS CR supported by the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large RI project LM2023050. Computational resources were provided by the e-INFRA CZ project (ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna Retzer and Jan Martinek for help with western blots, Anna Kampová for help with phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated by Univerzita Karlova, as part of the Wiley - CzechELib agreement.","issue":"3","scopus_import":"1","_id":"19423","has_accepted_license":"1","doi":"10.1111/nph.70019","author":[{"full_name":"Kurtović, K","last_name":"Kurtović","first_name":"K"},{"first_name":"S","full_name":"Vosolsobě, S","last_name":"Vosolsobě"},{"last_name":"Nedvěd","full_name":"Nedvěd, D","first_name":"D"},{"first_name":"K","full_name":"Müller, K","last_name":"Müller"},{"first_name":"PI","full_name":"Dobrev, PI","last_name":"Dobrev"},{"full_name":"Schmidt, V","last_name":"Schmidt","first_name":"V"},{"first_name":"P","last_name":"Piszczek","full_name":"Piszczek, P"},{"first_name":"A","last_name":"Kuhn","full_name":"Kuhn, A"},{"full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan","first_name":"Adrijana"},{"first_name":"TJ","last_name":"Fisher","full_name":"Fisher, TJ"},{"last_name":"Weijers","full_name":"Weijers, D","first_name":"D"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"JL","full_name":"Bowman, JL","last_name":"Bowman"},{"full_name":"Petrášek, J","last_name":"Petrášek","first_name":"J"}],"date_created":"2025-03-19T09:45:11Z","department":[{"_id":"JiFr"}],"publication":"New Phytologist","status":"public","publication_status":"published","day":"01","date_updated":"2025-09-30T11:11:18Z","oa_version":"Published Version","article_processing_charge":"Yes (via OA deal)","date_published":"2025-05-01T00:00:00Z","ddc":["580"],"title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","abstract":[{"text":"Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii.\r\nHere, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.","lang":"eng"}],"file":[{"date_updated":"2025-04-16T08:03:36Z","access_level":"open_access","file_size":12841729,"relation":"main_file","success":1,"creator":"dernst","date_created":"2025-04-16T08:03:36Z","content_type":"application/pdf","file_name":"2025_NewPhytologist_Kurtovic.pdf","checksum":"861c9bf47e7a7766ed03e6d85bd4f6dc","file_id":"19571"}],"citation":{"ama":"Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>. 2025;246(3):1066-1083. doi:<a href=\"https://doi.org/10.1111/nph.70019\">10.1111/nph.70019</a>","apa":"Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V., … Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.70019\">https://doi.org/10.1111/nph.70019</a>","ieee":"K. Kurtović <i>et al.</i>, “The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii,” <i>New Phytologist</i>, vol. 246, no. 3. Wiley, pp. 1066–1083, 2025.","chicago":"Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek, et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/nph.70019\">https://doi.org/10.1111/nph.70019</a>.","ista":"Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025. The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.","mla":"Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” <i>New Phytologist</i>, vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:<a href=\"https://doi.org/10.1111/nph.70019\">10.1111/nph.70019</a>.","short":"K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P. Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J. Petrášek, New Phytologist 246 (2025) 1066–1083."},"pmid":1,"project":[{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"article_type":"original","oa":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Wiley","volume":246,"intvolume":"       246","OA_place":"publisher","publication_identifier":{"issn":["1469-8137"]},"quality_controlled":"1","external_id":{"pmid":["40047465"],"isi":["001438711600001"]},"month":"05","type":"journal_article","page":"1066-1083"},{"_id":"19728","has_accepted_license":"1","doi":"10.1073/pnas.2427315122","acknowledgement":"This work was supported by the European Union’s Horizon 2020 research and innovation Programme (European Research Council grant agreement number 742985), Austrian Science Fund (FWF, grant number I 3630-B25), (Institute of Science and Technology Austria) Fellow program, the Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program (QCYRCXM-2022-237), the Fundamental Research Funds for Northwest A&F University and partly supported by the open funds of the State Key Laboratory of Plant Environmental Resilience (SKLPERKF2416). We also thank the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly Dr. Ningjuan Fan for technical assistance.","issue":"20","scopus_import":"1","author":[{"last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","first_name":"Yuzhou","orcid":"0000-0003-2627-6956"},{"first_name":"Zhulatai","last_name":"Bao","full_name":"Bao, Zhulatai"},{"first_name":"Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","full_name":"Smoljan, Adrijana"},{"full_name":"Liu, Yifan","last_name":"Liu","first_name":"Yifan"},{"full_name":"Wang, Huihui","last_name":"Wang","first_name":"Huihui"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2025-05-25T22:16:43Z","department":[{"_id":"JiFr"}],"isi":1,"article_number":"e2427315122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2025","related_material":{"link":[{"url":"https://ista.ac.at/en/news/how-roots-forage-for-water/","description":"News on ISTA website","relation":"press_release"}]},"language":[{"iso":"eng"}],"OA_type":"hybrid","corr_author":"1","file_date_updated":"2025-05-28T08:04:50Z","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I03630"}],"article_type":"original","file":[{"creator":"dernst","content_type":"application/pdf","date_created":"2025-05-28T08:04:50Z","file_name":"2025_PNAS_Zhang.pdf","checksum":"f70ff35054561b27a463ba279d1795dc","file_id":"19750","access_level":"open_access","file_size":8266672,"date_updated":"2025-05-28T08:04:50Z","relation":"main_file","success":1}],"pmid":1,"citation":{"ieee":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, and J. Friml, “Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 20. National Academy of Sciences, 2025.","ista":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. 2025. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings of the National Academy of Sciences. 122(20), e2427315122.","chicago":"Zhang, Yuzhou, Zhulatai Bao, Adrijana Smoljan, Yifan Liu, Huihui Wang, and Jiří Friml. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2427315122\">https://doi.org/10.1073/pnas.2427315122</a>.","mla":"Zhang, Yuzhou, et al. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism and Hydrotropism.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 20, e2427315122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2427315122\">10.1073/pnas.2427315122</a>.","short":"Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, J. Friml, Proceedings of the National Academy of Sciences 122 (2025).","ama":"Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(20). doi:<a href=\"https://doi.org/10.1073/pnas.2427315122\">10.1073/pnas.2427315122</a>","apa":"Zhang, Y., Bao, Z., Smoljan, A., Liu, Y., Wang, H., &#38; Friml, J. (2025). Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2427315122\">https://doi.org/10.1073/pnas.2427315122</a>"},"quality_controlled":"1","type":"journal_article","month":"05","external_id":{"pmid":["40372432"],"isi":["001496347500001"]},"volume":122,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"National Academy of Sciences","oa":1,"OA_place":"publisher","intvolume":"       122","publication_identifier":{"eissn":["1091-6490"]},"date_updated":"2026-05-20T08:34:21Z","day":"20","APC_amount":"5937,40 EUR","oa_version":"Published Version","date_published":"2025-05-20T00:00:00Z","article_processing_charge":"Yes (in subscription journal)","publication":"Proceedings of the National Academy of Sciences","publication_status":"published","status":"public","ec_funded":1,"abstract":[{"lang":"eng","text":"Root system integrates multiple environmental cues, chiefly gravity and soil humidity, to anchor plants in soil and forage for water. While the mechanism of auxin-mediated root gravitropism is comparably well-understood, the root’s capability to grow toward moist soil for water uptake and drought avoidance, termed root hydrotropism, remains largely mysterious. Here, we provide key insights into the mechanism of hydrotropic growth and assign a role to the master regulator of hydrotropism, MIZU-KUSSEI 1 (MIZ1). We show that efficient hydrotropism requires the attenuation of antagonistically acting gravitropism, which is inhibited under drought conditions. Drought stress interferes with subcellular trafficking and the lateral mobility of PIN auxin transporters, which are polarly localized at the root cell plasma membranes. This leads to defects in PIN2 polarity and gravity-induced polarization of PIN3, ultimately inhibiting gravity-induced auxin redistribution and root bending. The miz1 mutant is defective in all these regulations, and in support of MIZ1’s action on PINs, pin mutations rescue the hydrotropic defects in the miz1 mutant. These observations identify a mechanism for how drought via MIZ1 attenuates gravitropism to promote root hydrotropism for efficient water foraging under drought conditions."}],"ddc":["580"],"title":"Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism"},{"ddc":["580"],"title":"Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development","abstract":[{"text":"Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development.","lang":"eng"}],"publication":"Plant Journal","publication_status":"published","status":"public","article_processing_charge":"Yes (via OA deal)","date_published":"2025-08-01T00:00:00Z","date_updated":"2026-04-07T11:52:02Z","day":"01","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"oa_version":"Published Version","OA_place":"publisher","intvolume":"       123","publication_identifier":{"eissn":["1365-313X"],"issn":["0960-7412"]},"oa":1,"tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"volume":123,"publisher":"Wiley","type":"journal_article","month":"08","external_id":{"pmid":["40782342"],"isi":["001547884300001"]},"quality_controlled":"1","file":[{"date_updated":"2025-09-01T14:09:31Z","access_level":"open_access","file_size":5791111,"relation":"main_file","success":1,"creator":"dernst","date_created":"2025-09-01T14:09:31Z","content_type":"application/pdf","file_name":"2025_PlantJournal_Babic.pdf","checksum":"1cdc3341d2d23101abca72521f1f23cb","file_id":"20264"}],"citation":{"ieee":"D. Babic <i>et al.</i>, “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” <i>Plant Journal</i>, vol. 123, no. 3. Wiley, 2025.","ista":"Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396.","chicago":"Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>.","mla":"Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>, vol. 123, no. 3, e70396, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>.","short":"D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).","ama":"Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. 2025;123(3). doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>","apa":"Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>"},"pmid":1,"project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"article_type":"original","file_date_updated":"2025-09-01T14:09:31Z","corr_author":"1","OA_type":"hybrid","language":[{"iso":"eng"}],"year":"2025","related_material":{"record":[{"relation":"dissertation_contains","id":"20362","status":"public"}]},"isi":1,"article_number":"e70396","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","PlanS_conform":"1","date_created":"2025-08-17T22:01:36Z","department":[{"_id":"EvBe"},{"_id":"JiFr"},{"_id":"GradSch"}],"author":[{"full_name":"Babic, David","last_name":"Babic","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","first_name":"David"},{"orcid":"0000-0002-9357-9415","first_name":"Rashed","full_name":"Abualia, Rashed","last_name":"Abualia","id":"4827E134-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Fiedler, Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986","last_name":"Fiedler","first_name":"Lukas"},{"full_name":"Qi, Linlin","last_name":"Qi","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","orcid":"0000-0001-5187-8401","first_name":"Linlin"},{"full_name":"Tellier, Frédérique","last_name":"Tellier","first_name":"Frédérique"},{"first_name":"Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan","full_name":"Smoljan, Adrijana"},{"first_name":"Hana","full_name":"Rakusova, Hana","last_name":"Rakusova","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA"},{"first_name":"Petr","full_name":"Valošek, Petr","id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87","last_name":"Valošek"},{"last_name":"Han","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin","first_name":"Huibin"},{"full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"},{"last_name":"Faure","full_name":"Faure, Jean Denis","first_name":"Jean Denis"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"scopus_import":"1","acknowledgement":"We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","issue":"3","has_accepted_license":"1","doi":"10.1111/tpj.70396","_id":"20187"}]