---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '22301'
abstract:
- lang: eng
text: Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth
and development in land plants, but its physiological role in chlorophyte algae
remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella
sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent
manner. Low IAA concentrations promoted growth by accelerating the onset of cell
division without affecting cell size, whereas higher concentrations inhibited
proliferation. Radiotracer assays showed that all three species take up and release
IAA across the plasma membrane through a combination of passive diffusion and
energy-dependent, saturable processes. Competition by excess unlabeled natural
and synthetic auxins further supported the presence of carrier-mediated transport
with broad substrate recognition. Phylogenetic analyses identified potential PIN-like
auxin exporters in chlorophytes and other non-plant eukaryotes, and structural
modeling supported conservation of the overall PIN fold and predicted auxin-binding
residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured
cells, and Arabidopsis thaliana did not support a role for these proteins in directional
auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic
reticulum and showed limited or no transport activity in heterologous systems.
Together, these findings indicate that auxin responsiveness and basic cellular
auxin transport predate canonical PIN-mediated directional auxin export, which
appears to be a later innovation of the streptophyte lineage.
acknowledged_ssus:
- _id: Bio
- _id: NanoFab
acknowledgement: Research in the Friml group was supported by the European Research
Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian
Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from
the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional
support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG,
German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics
Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support
with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with
microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB
CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided
by Institute of Science and Technology Austria.
article_number: jipb.70309
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Sarah
full_name: Koutnik‐Abele, Sarah
last_name: Koutnik‐Abele
- first_name: Dmitrii
full_name: Vladimirtsev, Dmitrii
id: 60466724-5355-11ee-ae5a-fa55e8f99c3d
last_name: Vladimirtsev
- first_name: Petr
full_name: Klíma, Petr
last_name: Klíma
- first_name: Anita
full_name: Bírošíková, Anita
last_name: Bírošíková
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Maximilian
full_name: Schuster, Maximilian
id: 37e65def-d415-11eb-ae59-a7b67be103db
last_name: Schuster
- first_name: Katarina
full_name: Kurtović, Katarina
last_name: Kurtović
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated
transport in chlorophyte algae. Journal of Integrative Plant Biology. 2026.
doi:10.1111/jipb.70309
apa: Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A.,
Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte
algae. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.70309
chicago: Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma,
Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated
Transport in Chlorophyte Algae.” Journal of Integrative Plant Biology.
Wiley, 2026. https://doi.org/10.1111/jipb.70309.
ieee: A. Smoljan et al., “Auxin response and PIN‐mediated transport in chlorophyte
algae,” Journal of Integrative Plant Biology. Wiley, 2026.
ista: Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y,
Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin
response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative
Plant Biology., jipb. 70309.
mla: Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte
Algae.” Journal of Integrative Plant Biology, jipb. 70309, Wiley, 2026,
doi:10.1111/jipb.70309.
short: A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y.
Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml,
Journal of Integrative Plant Biology (2026).
corr_author: '1'
das_tickbox: '0'
date_created: 2026-07-13T10:44:55Z
date_published: 2026-06-10T00:00:00Z
date_updated: 2026-07-13T14:26:31Z
day: '10'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
- _id: NanoFab
- _id: Bio
doi: 10.1111/jipb.70309
external_id:
pmid:
- '42271607'
fulldoi: https://doi.org/10.1111/jipb.70309
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.70309
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Auxin response and PIN‐mediated transport in chlorophyte algae
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_type: closed access
_id: '20818'
abstract:
- lang: eng
text: "This study demonstrates that Marchantia non-canonical PINs are predominantly
localized to the plasma membrane, with MpPINX and MpPINW exhibiting asymmetric
distribution.\r\nA newly identified miniW domain within the MpPINW hydrophilic
loop governs subcellular trafficking and asymmetric PM localization of non-canonical
PINs in Marchantia."
acknowledgement: The authors sincerely thank Dr. Shutang Tan for experimental support
and Dr. Barbara Kloeckener Gruissem for critical reading and constructive advice
on the manuscript. This study was supported by the European Research Council Advanced
Grant (ETAP-742985 to H.T. and J.F.), by the Ministry of Science and Technology
(grant 112-2636-B-005-001- to K.-J.L.), and by the Ministry of Education (grant
MOE-109-YSFAG-0006-001-P1 to K.-J.L.).
article_processing_charge: No
article_type: comment
author:
- first_name: Han
full_name: Tang, Han
id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
last_name: Tang
orcid: 0000-0001-6152-6637
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Minxia
full_name: Zou, Minxia
id: 5c243f41-03f3-11ec-841c-96faf48a7ef9
last_name: Zou
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Kuan Ju
full_name: Lu, Kuan Ju
last_name: Lu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. The miniW domain directs
polarized membrane localization of non-canonical PINs in Marchantia polymorpha.
Plant Cell and Environment. 2026;49(3):1505-1508. doi:10.1111/pce.70295
apa: Tang, H., Smoljan, A., Zou, M., Zhang, Y., Lu, K. J., & Friml, J. (2026).
The miniW domain directs polarized membrane localization of non-canonical PINs
in Marchantia polymorpha. Plant Cell and Environment. Wiley. https://doi.org/10.1111/pce.70295
chicago: Tang, Han, Adrijana Smoljan, Minxia Zou, Yuzhou Zhang, Kuan Ju Lu, and
Jiří Friml. “The MiniW Domain Directs Polarized Membrane Localization of Non-Canonical
PINs in Marchantia Polymorpha.” Plant Cell and Environment. Wiley, 2026.
https://doi.org/10.1111/pce.70295.
ieee: H. Tang, A. Smoljan, M. Zou, Y. Zhang, K. J. Lu, and J. Friml, “The miniW
domain directs polarized membrane localization of non-canonical PINs in Marchantia
polymorpha,” Plant Cell and Environment, vol. 49, no. 3. Wiley, pp. 1505–1508,
2026.
ista: Tang H, Smoljan A, Zou M, Zhang Y, Lu KJ, Friml J. 2026. The miniW domain
directs polarized membrane localization of non-canonical PINs in Marchantia polymorpha.
Plant Cell and Environment. 49(3), 1505–1508.
mla: Tang, Han, et al. “The MiniW Domain Directs Polarized Membrane Localization
of Non-Canonical PINs in Marchantia Polymorpha.” Plant Cell and Environment,
vol. 49, no. 3, Wiley, 2026, pp. 1505–08, doi:10.1111/pce.70295.
short: H. Tang, A. Smoljan, M. Zou, Y. Zhang, K.J. Lu, J. Friml, Plant Cell and
Environment 49 (2026) 1505–1508.
das_tickbox: '1'
dataavailabilitystatement: The data that support the findings of this study are available
from the corresponding author upon reasonable request.
date_created: 2025-12-14T23:02:05Z
date_published: 2026-03-01T00:00:00Z
date_updated: 2026-07-27T10:27:47Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/pce.70295
ec_funded: 1
external_id:
pmid:
- '41340422'
fulldoi: https://doi.org/10.1111/pce.70295
intvolume: ' 49'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 1505-1508
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Cell and Environment
publication_identifier:
eissn:
- 1365-3040
issn:
- 0140-7791
publication_status: published
publisher: Wiley
quality_controlled: '1'
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: The miniW domain directs polarized membrane localization of non-canonical PINs
in Marchantia polymorpha
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 49
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '19423'
abstract:
- lang: eng
text: "Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic
roles in land plants, but its function and transport mechanisms in algae remain
poorly understood. We therefore aimed to explore the role of IAA in a complex,
streptophyte algae Chara braunii.\r\nHere, we described novel responses of C.
braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa
and CbPINc. We determined their localization in C. braunii using epitope-specific
antibodies and tested their function in heterologous land plant models. Further,
using phosphoproteomic analysis, we identified IAA-induced phosphorylation events.\r\nThe
thallus regeneration assay showed that IAA promotes thallus elongation and side
branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence
on the plasma membrane of vegetative and generative cells of C. braunii. However,
functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin
transport, whereas CbPINc does not. The IAA is effective in the acceleration of
cytoplasmic streaming and the phosphorylation of evolutionary conserved targets
such as homolog of RAF-like kinase.\r\nThese findings suggest that, although canonical
PIN-mediated auxin transport mechanisms might not be fully conserved in Chara,
IAA is involved in morphogenesis and fast signaling processes."
acknowledgement: 'This work was supported by funding from the Czech Science Foundation
project no. 20-13587S to JP and SV, Charles University Grant Agency projects no.
289523 to KK and no. 393422 to VS, a DOC fellowship of the Austrian Academy of Sciences
to AS, and the Austrian Science Fund (FWF): I 6123-B to JF. The authors acknowledge
the Imaging Facility of the Institute of Experimental Botany AS CR supported by
the MEYS CR (LM2023050 Czech-BioImaging), the Czech Academy of Sciences and IEB
AS CR, and Viničná Microscopy Core Facility cofinanced by the Czech-BioImaging large
RI project LM2023050. Computational resources were provided by the e-INFRA CZ project
(ID:90254), supported by the MEYS CR. The authors would like to thank Ilse Foissner
and Margit Höftberger for discussing details of immunostaining protocol, Katarzyna
Retzer and Jan Martinek for help with western blots, Anna Kampová for help with
phosphoproteome sampling, Anja Holzhausen and MadLAnd for providing Chara braunii
strain S276, and Roman Skokan for valuable discussion. Open access publishing facilitated
by Univerzita Karlova, as part of the Wiley - CzechELib agreement.'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: K
full_name: Kurtović, K
last_name: Kurtović
- first_name: S
full_name: Vosolsobě, S
last_name: Vosolsobě
- first_name: D
full_name: Nedvěd, D
last_name: Nedvěd
- first_name: K
full_name: Müller, K
last_name: Müller
- first_name: PI
full_name: Dobrev, PI
last_name: Dobrev
- first_name: V
full_name: Schmidt, V
last_name: Schmidt
- first_name: P
full_name: Piszczek, P
last_name: Piszczek
- first_name: A
full_name: Kuhn, A
last_name: Kuhn
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: TJ
full_name: Fisher, TJ
last_name: Fisher
- first_name: D
full_name: Weijers, D
last_name: Weijers
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: JL
full_name: Bowman, JL
last_name: Bowman
- first_name: J
full_name: Petrášek, J
last_name: Petrášek
citation:
ama: Kurtović K, Vosolsobě S, Nedvěd D, et al. The role of indole-3-acetic acid
and characterization of PIN transporters in complex streptophyte alga Chara braunii.
New Phytologist. 2025;246(3):1066-1083. doi:10.1111/nph.70019
apa: Kurtović, K., Vosolsobě, S., Nedvěd, D., Müller, K., Dobrev, P., Schmidt, V.,
… Petrášek, J. (2025). The role of indole-3-acetic acid and characterization of
PIN transporters in complex streptophyte alga Chara braunii. New Phytologist.
Wiley. https://doi.org/10.1111/nph.70019
chicago: Kurtović, K, S Vosolsobě, D Nedvěd, K Müller, PI Dobrev, V Schmidt, P Piszczek,
et al. “The Role of Indole-3-Acetic Acid and Characterization of PIN Transporters
in Complex Streptophyte Alga Chara Braunii.” New Phytologist. Wiley, 2025.
https://doi.org/10.1111/nph.70019.
ieee: K. Kurtović et al., “The role of indole-3-acetic acid and characterization
of PIN transporters in complex streptophyte alga Chara braunii,” New Phytologist,
vol. 246, no. 3. Wiley, pp. 1066–1083, 2025.
ista: Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev P, Schmidt V, Piszczek
P, Kuhn A, Smoljan A, Fisher T, Weijers D, Friml J, Bowman J, Petrášek J. 2025.
The role of indole-3-acetic acid and characterization of PIN transporters in complex
streptophyte alga Chara braunii. New Phytologist. 246(3), 1066–1083.
mla: Kurtović, K., et al. “The Role of Indole-3-Acetic Acid and Characterization
of PIN Transporters in Complex Streptophyte Alga Chara Braunii.” New Phytologist,
vol. 246, no. 3, Wiley, 2025, pp. 1066–83, doi:10.1111/nph.70019.
short: K. Kurtović, S. Vosolsobě, D. Nedvěd, K. Müller, P. Dobrev, V. Schmidt, P.
Piszczek, A. Kuhn, A. Smoljan, T. Fisher, D. Weijers, J. Friml, J. Bowman, J.
Petrášek, New Phytologist 246 (2025) 1066–1083.
date_created: 2025-03-19T09:45:11Z
date_published: 2025-05-01T00:00:00Z
date_updated: 2025-09-30T11:11:18Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.70019
external_id:
isi:
- '001438711600001'
pmid:
- '40047465'
file:
- access_level: open_access
checksum: 861c9bf47e7a7766ed03e6d85bd4f6dc
content_type: application/pdf
creator: dernst
date_created: 2025-04-16T08:03:36Z
date_updated: 2025-04-16T08:03:36Z
file_id: '19571'
file_name: 2025_NewPhytologist_Kurtovic.pdf
file_size: 12841729
relation: main_file
success: 1
file_date_updated: 2025-04-16T08:03:36Z
fulldoi: https://doi.org/10.1111/nph.70019
has_accepted_license: '1'
intvolume: ' 246'
isi: 1
issue: '3'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1066-1083
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: New Phytologist
publication_identifier:
issn:
- 1469-8137
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: The role of indole-3-acetic acid and characterization of PIN transporters in
complex streptophyte alga Chara braunii
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 246
year: '2025'
...
---
APC_amount: 5937,40 EUR
OA_place: publisher
OA_type: hybrid
_id: '19728'
abstract:
- lang: eng
text: Root system integrates multiple environmental cues, chiefly gravity and soil
humidity, to anchor plants in soil and forage for water. While the mechanism of
auxin-mediated root gravitropism is comparably well-understood, the root’s capability
to grow toward moist soil for water uptake and drought avoidance, termed root
hydrotropism, remains largely mysterious. Here, we provide key insights into the
mechanism of hydrotropic growth and assign a role to the master regulator of hydrotropism,
MIZU-KUSSEI 1 (MIZ1). We show that efficient hydrotropism requires the attenuation
of antagonistically acting gravitropism, which is inhibited under drought conditions.
Drought stress interferes with subcellular trafficking and the lateral mobility
of PIN auxin transporters, which are polarly localized at the root cell plasma
membranes. This leads to defects in PIN2 polarity and gravity-induced polarization
of PIN3, ultimately inhibiting gravity-induced auxin redistribution and root bending.
The miz1 mutant is defective in all these regulations, and in support of MIZ1’s
action on PINs, pin mutations rescue the hydrotropic defects in the miz1 mutant.
These observations identify a mechanism for how drought via MIZ1 attenuates gravitropism
to promote root hydrotropism for efficient water foraging under drought conditions.
acknowledgement: This work was supported by the European Union’s Horizon 2020 research
and innovation Programme (European Research Council grant agreement number 742985),
Austrian Science Fund (FWF, grant number I 3630-B25), (Institute of Science and
Technology Austria) Fellow program, the Qin Chuangyuan High-level Innovation and
Entrepreneurship Talent Program (QCYRCXM-2022-237), the Fundamental Research Funds
for Northwest A&F University and partly supported by the open funds of the State
Key Laboratory of Plant Environmental Resilience (SKLPERKF2416). We also thank the
Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F
University, particularly Dr. Ningjuan Fan for technical assistance.
article_number: e2427315122
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Zhulatai
full_name: Bao, Zhulatai
last_name: Bao
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Yifan
full_name: Liu, Yifan
last_name: Liu
- first_name: Huihui
full_name: Wang, Huihui
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. Foraging for water by MIZ1-mediated
antagonism between root gravitropism and hydrotropism. Proceedings of the National
Academy of Sciences. 2025;122(20). doi:10.1073/pnas.2427315122
apa: Zhang, Y., Bao, Z., Smoljan, A., Liu, Y., Wang, H., & Friml, J. (2025).
Foraging for water by MIZ1-mediated antagonism between root gravitropism and hydrotropism.
Proceedings of the National Academy of Sciences. National Academy of Sciences.
https://doi.org/10.1073/pnas.2427315122
chicago: Zhang, Yuzhou, Zhulatai Bao, Adrijana Smoljan, Yifan Liu, Huihui Wang,
and Jiří Friml. “Foraging for Water by MIZ1-Mediated Antagonism between Root Gravitropism
and Hydrotropism.” Proceedings of the National Academy of Sciences. National
Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2427315122.
ieee: Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, and J. Friml, “Foraging for
water by MIZ1-mediated antagonism between root gravitropism and hydrotropism,”
Proceedings of the National Academy of Sciences, vol. 122, no. 20. National
Academy of Sciences, 2025.
ista: Zhang Y, Bao Z, Smoljan A, Liu Y, Wang H, Friml J. 2025. Foraging for water
by MIZ1-mediated antagonism between root gravitropism and hydrotropism. Proceedings
of the National Academy of Sciences. 122(20), e2427315122.
mla: Zhang, Yuzhou, et al. “Foraging for Water by MIZ1-Mediated Antagonism between
Root Gravitropism and Hydrotropism.” Proceedings of the National Academy of
Sciences, vol. 122, no. 20, e2427315122, National Academy of Sciences, 2025,
doi:10.1073/pnas.2427315122.
short: Y. Zhang, Z. Bao, A. Smoljan, Y. Liu, H. Wang, J. Friml, Proceedings of the
National Academy of Sciences 122 (2025).
corr_author: '1'
date_created: 2025-05-25T22:16:43Z
date_published: 2025-05-20T00:00:00Z
date_updated: 2026-05-20T08:34:21Z
day: '20'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.2427315122
ec_funded: 1
external_id:
isi:
- '001496347500001'
pmid:
- '40372432'
file:
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checksum: f70ff35054561b27a463ba279d1795dc
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creator: dernst
date_created: 2025-05-28T08:04:50Z
date_updated: 2025-05-28T08:04:50Z
file_id: '19750'
file_name: 2025_PNAS_Zhang.pdf
file_size: 8266672
relation: main_file
success: 1
file_date_updated: 2025-05-28T08:04:50Z
fulldoi: https://doi.org/10.1073/pnas.2427315122
has_accepted_license: '1'
intvolume: ' 122'
isi: 1
issue: '20'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/how-roots-forage-for-water/
scopus_import: '1'
status: public
title: Foraging for water by MIZ1-mediated antagonism between root gravitropism and
hydrotropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 122
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20187'
abstract:
- lang: eng
text: Very long-chain fatty acids (VLCFAs), being constituents of different types
of lipids, are critical factors in plant development, presumably due to their
impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic
reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase
1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems
(esm) mutant, a viable kcr1 allele presumably affecting surface properties of
the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that
impacts VLCFAs. The kcr1-2 plants show severe defects during different stages
of development, which all correlate with defects in polar localization and subcellular
trafficking of PIN auxin transporters and resulting asymmetric auxin distribution.
Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests
that KCR1 plays a role in delineating boundaries around meristematic and specialized
differentiating tissues, including root and shoot meristems, initiating lateral
roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced
VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful
tool to study VLCFA roles in plant development and highlights VLCFAs as critical
developmental factors at the interface of cell polarity and tissue development.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy
(especially Vanessa Zheden for technical assistance) and Life Science (in particular
Dorota Jaworska) facilities at ISTA for their continuous support. Authors would
like to thank Michelle Gallei for advice during the generation of the transgenic
lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help
and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and
root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski
for advice on abiotic stress experiments. We would like to thank Dolf Weijers for
the SOSEKI (SOK) marker line seeds. This work has benefited from the support of
IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian
Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province
(20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant
Sciences-SPS (ANR-17-EUR-0007)."
article_number: e70396
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: David
full_name: Babic, David
id: db566d23-f6e0-11ea-865d-e6f270e968e7
last_name: Babic
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Frédérique
full_name: Tellier, Frédérique
last_name: Tellier
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Hana
full_name: Rakusova, Hana
id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
last_name: Rakusova
- first_name: Petr
full_name: Valošek, Petr
id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
last_name: Valošek
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jean Denis
full_name: Faure, Jean Denis
last_name: Faure
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty
acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396
apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml,
J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis
auxin-mediated embryonic and post-embryonic development. Plant Journal.
Wiley. https://doi.org/10.1111/tpj.70396
chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier,
Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty
Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic
Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396.
ieee: D. Babic et al., “Biosynthesis of very long-chain fatty acids is required
for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant
Journal, vol. 123, no. 3. Wiley, 2025.
ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek
P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain
fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 123(3), e70396.
mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required
for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant
Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396.
short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova,
P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).
corr_author: '1'
date_created: 2025-08-17T22:01:36Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-04-07T11:52:02Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
- _id: GradSch
doi: 10.1111/tpj.70396
external_id:
isi:
- '001547884300001'
pmid:
- '40782342'
file:
- access_level: open_access
checksum: 1cdc3341d2d23101abca72521f1f23cb
content_type: application/pdf
creator: dernst
date_created: 2025-09-01T14:09:31Z
date_updated: 2025-09-01T14:09:31Z
file_id: '20264'
file_name: 2025_PlantJournal_Babic.pdf
file_size: 5791111
relation: main_file
success: 1
file_date_updated: 2025-09-01T14:09:31Z
fulldoi: https://doi.org/10.1111/tpj.70396
has_accepted_license: '1'
intvolume: ' 123'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: Plant Journal
publication_identifier:
eissn:
- 1365-313X
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
- id: '20362'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated
embryonic and post-embryonic development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 123
year: '2025'
...