---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21235'
abstract:
- lang: eng
text: The condensation of charged polymers is an important driver for the formation
of biomolecular condensates. Recent experiments suggest that this mechanism also
controls the clustering of eukaryotic chromosomes during the late stages of cell
division. In this process, interchromosome attraction is driven by the condensation
of cytoplasmic RNA and Ki-67, a charged intrinsically disordered protein that
coats the chromosomes as a brush. Attraction between chromosomes has been shown
to be specifically promoted by a localized charged patch on Ki-67, although the
physical mechanism remains unclear. To elucidate this process, we combine coarse-grained
simulations and analytical theory to study the RNA-mediated interaction between
charged polymer brushes on the chromosome surfaces. We show that the charged patch
on Ki-67 leads to interchromosome attraction via RNA bridging between the two
brushes, whereby the RNA preferentially interacts with the charged patches, leading
to stable, long-range forces. By contrast, if the brush is uniformly charged,
bridging is basically absent due to complete adsorption of RNA onto the brush.
Moreover, the RNA dynamics becomes caged in presence of the charged patch while
remaining diffusive with uniform charge. Our work sheds light on the physical
origin of chromosome clustering, while also suggesting a general mechanism for
cells to tune work production by biomolecular condensates via different charge
distributions.
acknowledgement: "This work was supported by the European Union’s Horizon 2020 research
and innovation programme (A.Š. and V.S., ERC grant Agreement No. 802960 to A.Š.,
I.P. and P.R.,\r\nMarie Skłodowska-Curie Grant Agreement No. 101034413), the German
Research Foundation (S.C-H. and A.H.-A., DFG Project No. 402723784 to S.C-H.), the
Vallee Scholarship\r\n(A.Š. and V.S.), the EMBO Young Investigator Programme (A.Š.),
and a Ph.D. fellowship from the Boehringer Ingelheim Fonds (A.H.-A.)."
article_number: '033010'
article_processing_charge: Yes
article_type: original
author:
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Paul
full_name: Robin, Paul
id: 48c58128-57b0-11ee-9095-dc28fd97fc1d
last_name: Robin
orcid: 0000-0002-5728-9189
- first_name: Ivan
full_name: Palaia, Ivan
id: 9c805cd2-4b75-11ec-a374-db6dd0ed57fa
last_name: Palaia
orcid: ' 0000-0002-8843-9485 '
- first_name: Alberto
full_name: Hernandez-Armendariz, Alberto
last_name: Hernandez-Armendariz
- first_name: Sara
full_name: Cuylen-Haering, Sara
last_name: Cuylen-Haering
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
citation:
ama: Sorichetti V, Robin P, Palaia I, Hernandez-Armendariz A, Cuylen-Haering S,
Šarić A. Charge distribution of the coating brush drives interchromosome attraction.
PRX Life. 2025;3(3). doi:10.1103/41fd-r847
apa: Sorichetti, V., Robin, P., Palaia, I., Hernandez-Armendariz, A., Cuylen-Haering,
S., & Šarić, A. (2025). Charge distribution of the coating brush drives interchromosome
attraction. PRX Life. American Physical Society. https://doi.org/10.1103/41fd-r847
chicago: Sorichetti, Valerio, Paul Robin, Ivan Palaia, Alberto Hernandez-Armendariz,
Sara Cuylen-Haering, and Anđela Šarić. “Charge Distribution of the Coating Brush
Drives Interchromosome Attraction.” PRX Life. American Physical Society,
2025. https://doi.org/10.1103/41fd-r847.
ieee: V. Sorichetti, P. Robin, I. Palaia, A. Hernandez-Armendariz, S. Cuylen-Haering,
and A. Šarić, “Charge distribution of the coating brush drives interchromosome
attraction,” PRX Life, vol. 3, no. 3. American Physical Society, 2025.
ista: Sorichetti V, Robin P, Palaia I, Hernandez-Armendariz A, Cuylen-Haering S,
Šarić A. 2025. Charge distribution of the coating brush drives interchromosome
attraction. PRX Life. 3(3), 033010.
mla: Sorichetti, Valerio, et al. “Charge Distribution of the Coating Brush Drives
Interchromosome Attraction.” PRX Life, vol. 3, no. 3, 033010, American
Physical Society, 2025, doi:10.1103/41fd-r847.
short: V. Sorichetti, P. Robin, I. Palaia, A. Hernandez-Armendariz, S. Cuylen-Haering,
A. Šarić, PRX Life 3 (2025).
corr_author: '1'
date_created: 2026-02-16T14:50:32Z
date_published: 2025-08-11T00:00:00Z
date_updated: 2026-02-17T11:16:26Z
day: '11'
ddc:
- '570'
department:
- _id: AnSa
- _id: EdHa
doi: 10.1103/41fd-r847
ec_funded: 1
file:
- access_level: open_access
checksum: 1702b9bdbfd902a7c08aa4f1479b390d
content_type: application/pdf
creator: dernst
date_created: 2026-02-17T11:12:30Z
date_updated: 2026-02-17T11:12:30Z
file_id: '21287'
file_name: 2025_PRXLife_Sorichetti.pdf
file_size: 3732843
relation: main_file
success: 1
file_date_updated: 2026-02-17T11:12:30Z
has_accepted_license: '1'
intvolume: ' 3'
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
call_identifier: H2020
grant_number: '802960'
name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
name: EMBO Young Investigator Program - Andela Saric
publication: PRX Life
publication_identifier:
eissn:
- 2835-8279
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
status: public
title: Charge distribution of the coating brush drives interchromosome attraction
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21256'
abstract:
- lang: eng
text: Collagen IV is one of the main components of the basement membrane, a layer
of material that lines the majority of tissues in multicellular organisms. Collagen
IV molecules assemble into networks, providing stiffness and elasticity to tissues
and informing cell and organ shape, especially during development. In this work,
we develop two coarse-grained models for collagen IV molecules that retain biochemical
bond specificity and coarse grain at different length scales. Through molecular-dynamics
simulations, we test the assembly and mechanics of the resulting networks and
measure their response to strain in terms of stress, microscopic alignment, and
bond dynamics. Within the basement membrane, collagen IV networks rearrange by
molecule turnover, which affects tissue organization and can be linked with enzyme
activity. Here we explore network rearrangements via bond remodeling, the process
of breaking and remaking of bonds between network molecules. We then investigate
the effects of active (enzymatic) bond remodeling. We find that this nonequilibrium
remodeling allows a network to keep its integrity under strain, while relaxing
fully over a variety of timescales, a dynamic response that is unavailable to
networks undergoing equilibrium remodeling.
acknowledgement: This work received funding from the European Research Council under
the European Union's Horizon 2020 research and innovation program through Grant
Agreement No. 802960 (B.M., V.S., I.P., and A.Š.), the European Union's Horizon
2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement
No. 101034413 (I.P.), the NOMIS Foundation (F.P.-V.), the National Centre for the
Replacement, Refinement and Reduction of Animals in Research Grant No. NC/T002425/1
(N.K.), Leverhulme Trust project Grant No. RPG-2020-068 (N.K.), MRC Fellowship No.
MR/W027437/1 (Y.M.), a Lister Institute Research Prize (Y.M.) and EMBO Young Investigator
Programme (Y.M. and A.Š.).
article_number: '033019'
article_processing_charge: Yes
article_type: original
author:
- first_name: Billie
full_name: Meadowcroft, Billie
id: a4725fd6-932b-11ed-81e2-c098c7f37ae1
last_name: Meadowcroft
orcid: 0000-0003-3441-1337
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Eryk
full_name: Ratajczyk, Eryk
last_name: Ratajczyk
- first_name: Fernanda L
full_name: Perez Verdugo, Fernanda L
id: 4ecec223-9070-11ef-a0a9-bc76077bea8d
last_name: Perez Verdugo
- first_name: Nargess
full_name: Khalilgharibi, Nargess
last_name: Khalilgharibi
- first_name: Yanlan
full_name: Mao, Yanlan
last_name: Mao
- first_name: Ivan
full_name: Palaia, Ivan
id: 9c805cd2-4b75-11ec-a374-db6dd0ed57fa
last_name: Palaia
orcid: ' 0000-0002-8843-9485 '
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
citation:
ama: Meadowcroft B, Sorichetti V, Ratajczyk E, et al. Nonequilibrium remodeling
of collagen IV networks in Silico. PRX Life. 2025;3. doi:10.1103/gdd5-rnh7
apa: Meadowcroft, B., Sorichetti, V., Ratajczyk, E., Perez Verdugo, F. L., Khalilgharibi,
N., Mao, Y., … Šarić, A. (2025). Nonequilibrium remodeling of collagen IV networks
in Silico. PRX Life. American Physical Society. https://doi.org/10.1103/gdd5-rnh7
chicago: Meadowcroft, Billie, Valerio Sorichetti, Eryk Ratajczyk, Fernanda L Perez
Verdugo, Nargess Khalilgharibi, Yanlan Mao, Ivan Palaia, and Anđela Šarić. “Nonequilibrium
Remodeling of Collagen IV Networks in Silico.” PRX Life. American Physical
Society, 2025. https://doi.org/10.1103/gdd5-rnh7.
ieee: B. Meadowcroft et al., “Nonequilibrium remodeling of collagen IV networks
in Silico,” PRX Life, vol. 3. American Physical Society, 2025.
ista: Meadowcroft B, Sorichetti V, Ratajczyk E, Perez Verdugo FL, Khalilgharibi
N, Mao Y, Palaia I, Šarić A. 2025. Nonequilibrium remodeling of collagen IV networks
in Silico. PRX Life. 3, 033019.
mla: Meadowcroft, Billie, et al. “Nonequilibrium Remodeling of Collagen IV Networks
in Silico.” PRX Life, vol. 3, 033019, American Physical Society, 2025,
doi:10.1103/gdd5-rnh7.
short: B. Meadowcroft, V. Sorichetti, E. Ratajczyk, F.L. Perez Verdugo, N. Khalilgharibi,
Y. Mao, I. Palaia, A. Šarić, PRX Life 3 (2025).
corr_author: '1'
date_created: 2026-02-16T15:55:03Z
date_published: 2025-09-05T00:00:00Z
date_updated: 2026-02-17T13:37:38Z
day: '05'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1103/gdd5-rnh7
ec_funded: 1
file:
- access_level: open_access
checksum: 04cae5231d97e533145c493880fadbd9
content_type: application/pdf
creator: dernst
date_created: 2026-02-17T13:36:01Z
date_updated: 2026-02-17T13:36:01Z
file_id: '21308'
file_name: 2025_PRXLife_Meadowcroft.pdf
file_size: 2277704
relation: main_file
success: 1
file_date_updated: 2026-02-17T13:36:01Z
has_accepted_license: '1'
intvolume: ' 3'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
call_identifier: H2020
grant_number: '802960'
name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
name: EMBO Young Investigator Program - Andela Saric
publication: PRX Life
publication_identifier:
eissn:
- 2835-8279
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
status: public
title: Nonequilibrium remodeling of collagen IV networks in Silico
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20530'
abstract:
- lang: eng
text: Cells must coordinate DNA segregation with cytokinesis to ensure that each
daughter cell inherits a complete genome. Here, we explore how DNA segregation
and division are mechanistically coupled in archaeal relatives of eukaryotes,
which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we
first describe the series of sequential changes in DNA organization that accompany
cell division in Sulfolobus, which computational modeling shows likely aid genome
segregation. Through a perturbation analysis we identify a regulatory checkpoint
which ensures that the compaction of the genome into two spatially segregated
nucleoids only occurs once cells have assembled a division ring—which also defines
the axis of DNA segregation. Finally, we show that DNA compaction and segregation
depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence
leads to bridging DNA. Taken together, these data show how regulatory checkpoints
like those operating in eukaryotes aid high-fidelity division in an archaeon.
acknowledgement: We thank Matthew Kenneth for his assistance with live cell imaging.
We thank Arthur Charles-Orszag and Dyche Mullins for generously gifting the SegA
and SegB antibodies, and Sonja-Verena Albers for gifting the CdvA-HA overexpression
plasmid. We thank the Light Microscopy and Flow Cytometry facilities at the MRC-LMB,
and all the core staff at the MRC-LMB for their support. We thank all members of
the Baum lab for helpful discussions. We would like to thank Magdalena Lechowska,
Gautam Dey, Laura Downie, and Iva Tolic for critical reading of the manuscript.
J.P. was supported by the Medical Research Council—Laboratory of Molecular Biology
(MC_UP_1201/27). A.C. was funded by an EMBO Postdoctoral fellowship (ALTF_1041-2021),
a Marie Sklodowska-Curie Individual Fellowship (101068523) provided by UKRI and
by the Wellcome Trust (222460/Z/21/Z). B.H. was supported by Wellcome Trust (203276/A/16/Z).
Y.-W.K. was supported by an EMBO postdoctoral fellowship (ALTF 903-2021) and by
the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27); S.F.
was supported by the Wellcome Trust (222460/Z/21/Z); B.B. received support from
the MRC LMB, the Wellcome Trust (203276/Z/16/Z) and (222460/Z/21/Z), the VW Foundation
(94933), and from the Gordon and Betty Moore Foundation’s Symbiosis in Aquatic Systems
Initiative (9346). V.S. and A.Š. acknowledge funding from the European Research
Council under the European Union’s Horizon 2020 research and innovation programme
(grant no.802960 to A.Š.), the Vallee Scholarship, and the EMBO Young Investigator
Programme (A.Š.). The collaborative work of A.Š.’s and B.B. teams was also supported
by a Moore–Simons Project on the Origin of the Eukaryotic Cell, Simons Foundation
735929LPI.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Joe
full_name: Parham, Joe
last_name: Parham
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Alice
full_name: Cezanne, Alice
last_name: Cezanne
- first_name: Sherman
full_name: Foo, Sherman
last_name: Foo
- first_name: Yin Wei
full_name: Kuo, Yin Wei
last_name: Kuo
- first_name: Baukje
full_name: Hoogenberg, Baukje
last_name: Hoogenberg
- first_name: Arthur
full_name: Radoux-Mergault, Arthur
last_name: Radoux-Mergault
- first_name: Eloise
full_name: Mawdesley, Eloise
last_name: Mawdesley
- first_name: Lydia Daniels
full_name: Gatward, Lydia Daniels
last_name: Gatward
- first_name: Jerome
full_name: Boulanger, Jerome
last_name: Boulanger
- first_name: Ulrike
full_name: Schulze, Ulrike
last_name: Schulze
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
- first_name: Buzz
full_name: Baum, Buzz
last_name: Baum
citation:
ama: Parham J, Sorichetti V, Cezanne A, et al. Temporal and spatial coordination
of DNA segregation and cell division in an archaeon. Proceedings of the National
Academy of Sciences. 2025;122(42):e2513939122. doi:10.1073/pnas.2513939122
apa: Parham, J., Sorichetti, V., Cezanne, A., Foo, S., Kuo, Y. W., Hoogenberg, B.,
… Baum, B. (2025). Temporal and spatial coordination of DNA segregation and cell
division in an archaeon. Proceedings of the National Academy of Sciences.
National Academy of Sciences. https://doi.org/10.1073/pnas.2513939122
chicago: Parham, Joe, Valerio Sorichetti, Alice Cezanne, Sherman Foo, Yin Wei Kuo,
Baukje Hoogenberg, Arthur Radoux-Mergault, et al. “Temporal and Spatial Coordination
of DNA Segregation and Cell Division in an Archaeon.” Proceedings of the National
Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2513939122.
ieee: J. Parham et al., “Temporal and spatial coordination of DNA segregation
and cell division in an archaeon,” Proceedings of the National Academy of Sciences,
vol. 122, no. 42. National Academy of Sciences, p. e2513939122, 2025.
ista: Parham J, Sorichetti V, Cezanne A, Foo S, Kuo YW, Hoogenberg B, Radoux-Mergault
A, Mawdesley E, Gatward LD, Boulanger J, Schulze U, Šarić A, Baum B. 2025. Temporal
and spatial coordination of DNA segregation and cell division in an archaeon.
Proceedings of the National Academy of Sciences. 122(42), e2513939122.
mla: Parham, Joe, et al. “Temporal and Spatial Coordination of DNA Segregation and
Cell Division in an Archaeon.” Proceedings of the National Academy of Sciences,
vol. 122, no. 42, National Academy of Sciences, 2025, p. e2513939122, doi:10.1073/pnas.2513939122.
short: J. Parham, V. Sorichetti, A. Cezanne, S. Foo, Y.W. Kuo, B. Hoogenberg, A.
Radoux-Mergault, E. Mawdesley, L.D. Gatward, J. Boulanger, U. Schulze, A. Šarić,
B. Baum, Proceedings of the National Academy of Sciences 122 (2025) e2513939122.
date_created: 2025-10-26T23:01:33Z
date_published: 2025-10-21T00:00:00Z
date_updated: 2026-02-16T12:32:31Z
day: '21'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1073/pnas.2513939122
ec_funded: 1
external_id:
isi:
- '001620648600001'
pmid:
- '41091768'
file:
- access_level: open_access
checksum: 3555d51f438d2e356039a9b697eac3ee
content_type: application/pdf
creator: dernst
date_created: 2025-10-27T08:12:59Z
date_updated: 2025-10-27T08:12:59Z
file_id: '20543'
file_name: 2025_PNAS_Parham.pdf
file_size: 2649194
relation: main_file
success: 1
file_date_updated: 2025-10-27T08:12:59Z
has_accepted_license: '1'
intvolume: ' 122'
isi: 1
issue: '42'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: e2513939122
pmid: 1
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
call_identifier: H2020
grant_number: '802960'
name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
name: EMBO Young Investigator Program - Andela Saric
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Temporal and spatial coordination of DNA segregation and cell division in an
archaeon
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 122
year: '2025'
...
---
DOAJ_listed: '1'
_id: '17050'
abstract:
- lang: eng
text: The process of polymer condensation, i.e., the formation of bonds between
reactive end groups, is ubiquitous in both industry and biology. Here we study
generic systems undergoing polymer condensation in competition with cyclization.
Using a generalized Smoluchowski theory, molecular dynamics simulations and experiments
with DNA and ATP-consuming T4 ligase, we find that this system displays a transition,
from a ring-dominated regime with finite-length chains at infinite time to a linear-polymers-dominated
one with chains that keep growing in time. Finally, we show that fluids prepared
close to the transition may have widely different compositions and rheology at
large condensation times.
acknowledgement: D.M. acknowledges the support of the Royal Society via a University
Research Fellowship. This project has received support from the European Research
Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Program
(Grant Agreement No. 947918 to D.M. and No. 677532 to M.L.). The authors acknowledge
insightful discussions with Daan Noordermeer and Antonio Valdes, who also kindly
gifted us with the 1288 plasmid.
article_number: '023189'
article_processing_charge: Yes
article_type: original
arxiv: 1
author:
- first_name: Maria
full_name: Panoukidou, Maria
last_name: Panoukidou
- first_name: Simon
full_name: Weir, Simon
last_name: Weir
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Yair Gutierrez
full_name: Fosado, Yair Gutierrez
last_name: Fosado
- first_name: Martin
full_name: Lenz, Martin
last_name: Lenz
- first_name: Davide
full_name: Michieletto, Davide
last_name: Michieletto
citation:
ama: Panoukidou M, Weir S, Sorichetti V, Fosado YG, Lenz M, Michieletto D. Runaway
transition in irreversible polymer condensation with cyclization. Physical
Review Research. 2024;6(2). doi:10.1103/PhysRevResearch.6.023189
apa: Panoukidou, M., Weir, S., Sorichetti, V., Fosado, Y. G., Lenz, M., & Michieletto,
D. (2024). Runaway transition in irreversible polymer condensation with cyclization.
Physical Review Research. American Physical Society. https://doi.org/10.1103/PhysRevResearch.6.023189
chicago: Panoukidou, Maria, Simon Weir, Valerio Sorichetti, Yair Gutierrez Fosado,
Martin Lenz, and Davide Michieletto. “Runaway Transition in Irreversible Polymer
Condensation with Cyclization.” Physical Review Research. American Physical
Society, 2024. https://doi.org/10.1103/PhysRevResearch.6.023189.
ieee: M. Panoukidou, S. Weir, V. Sorichetti, Y. G. Fosado, M. Lenz, and D. Michieletto,
“Runaway transition in irreversible polymer condensation with cyclization,” Physical
Review Research, vol. 6, no. 2. American Physical Society, 2024.
ista: Panoukidou M, Weir S, Sorichetti V, Fosado YG, Lenz M, Michieletto D. 2024.
Runaway transition in irreversible polymer condensation with cyclization. Physical
Review Research. 6(2), 023189.
mla: Panoukidou, Maria, et al. “Runaway Transition in Irreversible Polymer Condensation
with Cyclization.” Physical Review Research, vol. 6, no. 2, 023189, American
Physical Society, 2024, doi:10.1103/PhysRevResearch.6.023189.
short: M. Panoukidou, S. Weir, V. Sorichetti, Y.G. Fosado, M. Lenz, D. Michieletto,
Physical Review Research 6 (2024).
date_created: 2024-05-26T22:00:58Z
date_published: 2024-05-01T00:00:00Z
date_updated: 2025-05-14T09:32:40Z
day: '01'
ddc:
- '530'
department:
- _id: AnSa
doi: 10.1103/PhysRevResearch.6.023189
external_id:
arxiv:
- '2210.14010'
file:
- access_level: open_access
checksum: 63a962d49ef1e21a3367d265784df14b
content_type: application/pdf
creator: dernst
date_created: 2024-05-27T06:37:01Z
date_updated: 2024-05-27T06:37:01Z
file_id: '17055'
file_name: 2024_PhysicalReviewResearch_Panoukidou.pdf
file_size: 1409416
relation: main_file
success: 1
file_date_updated: 2024-05-27T06:37:01Z
has_accepted_license: '1'
intvolume: ' 6'
issue: '2'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
publication: Physical Review Research
publication_identifier:
eissn:
- 2643-1564
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Runaway transition in irreversible polymer condensation with cyclization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2024'
...
---
_id: '18072'
abstract:
- lang: eng
text: The individualization of chromosomes during early mitosis and their clustering
upon exit from cell division are two key transitions that ensure efficient segregation
of eukaryotic chromosomes. Both processes are regulated by the surfactant-like
protein Ki-67, but how Ki-67 achieves these diametric functions has remained unknown.
Here, we report that Ki-67 radically switches from a chromosome repellent to a
chromosome attractant during anaphase in human cells. We show that Ki-67 dephosphorylation
during mitotic exit and the simultaneous exposure of a conserved basic patch induce
the RNA-dependent formation of a liquid-like condensed phase on the chromosome
surface. Experiments and coarse-grained simulations support a model in which the
coalescence of chromosome surfaces, driven by co-condensation of Ki-67 and RNA,
promotes clustering of chromosomes. Our study reveals how the switch of Ki-67
from a surfactant to a liquid-like condensed phase can generate mechanical forces
during genome segregation that are required for re-establishing nuclear-cytoplasmic
compartmentalization after mitosis.
acknowledgement: We thank Daniel W. Gerlich for providing cell lines, the EMBL Advanced
Light Microscopy Facility (ALMF) for support, Christian H. Haering and Thomas Quail
for input on the manuscript, and Martina Dees for cloning several Ki-67 constructs.
This work was supported by the German Research Foundation (DFG project number 402723784)
and the Human Frontier Science Program (CDA00045/2019). A.H.-A. and A.B. have received
PhD fellowships from the Boehringer Ingelheim Fonds, V.S. and A.Š. were supported
by the European Research Council (ERC) under the European Union’s Horizon 2020 research
and innovation programme (grant no. 802960), and Y.H. was supported by a fellowship
from the EMBL interdisciplinary Postdoc (EIPOD) program (Marie Sklodowska-Curie
Actions, COFUND grant agreement 664726).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Alberto
full_name: Hernandez-Armendariz, Alberto
last_name: Hernandez-Armendariz
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Yuki
full_name: Hayashi, Yuki
last_name: Hayashi
- first_name: Zuzana
full_name: Koskova, Zuzana
last_name: Koskova
- first_name: Andreas
full_name: Brunner, Andreas
last_name: Brunner
- first_name: Jan
full_name: Ellenberg, Jan
last_name: Ellenberg
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
- first_name: Sara
full_name: Cuylen-Haering, Sara
last_name: Cuylen-Haering
citation:
ama: Hernandez-Armendariz A, Sorichetti V, Hayashi Y, et al. A liquid-like coat
mediates chromosome clustering during mitotic exit. Molecular Cell. 2024;84(17):P3254-3270.E9.
doi:10.1016/j.molcel.2024.07.022
apa: Hernandez-Armendariz, A., Sorichetti, V., Hayashi, Y., Koskova, Z., Brunner,
A., Ellenberg, J., … Cuylen-Haering, S. (2024). A liquid-like coat mediates chromosome
clustering during mitotic exit. Molecular Cell. Cell Press. https://doi.org/10.1016/j.molcel.2024.07.022
chicago: Hernandez-Armendariz, Alberto, Valerio Sorichetti, Yuki Hayashi, Zuzana
Koskova, Andreas Brunner, Jan Ellenberg, Anđela Šarić, and Sara Cuylen-Haering.
“A Liquid-like Coat Mediates Chromosome Clustering during Mitotic Exit.” Molecular
Cell. Cell Press, 2024. https://doi.org/10.1016/j.molcel.2024.07.022.
ieee: A. Hernandez-Armendariz et al., “A liquid-like coat mediates chromosome
clustering during mitotic exit,” Molecular Cell, vol. 84, no. 17. Cell
Press, p. P3254–3270.E9, 2024.
ista: Hernandez-Armendariz A, Sorichetti V, Hayashi Y, Koskova Z, Brunner A, Ellenberg
J, Šarić A, Cuylen-Haering S. 2024. A liquid-like coat mediates chromosome clustering
during mitotic exit. Molecular Cell. 84(17), P3254–3270.E9.
mla: Hernandez-Armendariz, Alberto, et al. “A Liquid-like Coat Mediates Chromosome
Clustering during Mitotic Exit.” Molecular Cell, vol. 84, no. 17, Cell
Press, 2024, p. P3254–3270.E9, doi:10.1016/j.molcel.2024.07.022.
short: A. Hernandez-Armendariz, V. Sorichetti, Y. Hayashi, Z. Koskova, A. Brunner,
J. Ellenberg, A. Šarić, S. Cuylen-Haering, Molecular Cell 84 (2024) P3254–3270.E9.
date_created: 2024-09-15T22:01:41Z
date_published: 2024-09-05T00:00:00Z
date_updated: 2025-09-08T09:23:02Z
day: '05'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1016/j.molcel.2024.07.022
ec_funded: 1
external_id:
isi:
- '001309051100001'
pmid:
- '39153474'
file:
- access_level: open_access
checksum: 3f360e0287b8ec79fb2b8b02b5070360
content_type: application/pdf
creator: dernst
date_created: 2024-09-16T07:38:38Z
date_updated: 2024-09-16T07:38:38Z
file_id: '18075'
file_name: 2024_MolecularCell_HernandezArmendariz.pdf
file_size: 11654644
relation: main_file
success: 1
file_date_updated: 2024-09-16T07:38:38Z
has_accepted_license: '1'
intvolume: ' 84'
isi: 1
issue: '17'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: P3254-3270.E9
pmid: 1
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
call_identifier: H2020
grant_number: '802960'
name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
publication: Molecular Cell
publication_identifier:
eissn:
- 1097-4164
issn:
- 1097-2765
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: A liquid-like coat mediates chromosome clustering during mitotic exit
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 84
year: '2024'
...
---
_id: '12705'
abstract:
- lang: eng
text: The elasticity of disordered and polydisperse polymer networks is a fundamental
problem of soft matter physics that is still open. Here, we self-assemble polymer
networks via simulations of a mixture of bivalent and tri- or tetravalent patchy
particles, which result in an exponential strand length distribution analogous
to that of experimental randomly cross-linked systems. After assembly, the network
connectivity and topology are frozen and the resulting system is characterized.
We find that the fractal structure of the network depends on the number density
at which the assembly has been carried out, but that systems with the same mean
valence and same assembly density have the same structural properties. Moreover,
we compute the long-time limit of the mean-squared displacement, also known as
the (squared) localization length, of the cross-links and of the middle monomers
of the strands, showing that the dynamics of long strands is well described by
the tube model. Finally, we find a relation connecting these two localization
lengths at high density and connect the cross-link localization length to the
shear modulus of the system.
acknowledgement: We thank Michael Lang for helpful discussions. We acknowledge financial
support from the European Research Council (ERC Consolidator Grant No. 681597, MIMIC)
and from LabEx NUMEV (Grant No. ANR-10-LABX-20) funded by the “Investissements d’Avenir”
French Government program, managed by the French National Research Agency (ANR).
W.K. is a senior member of the Institut Universitaire de France.
article_number: '074905'
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Andrea
full_name: Ninarello, Andrea
last_name: Ninarello
- first_name: José
full_name: Ruiz-Franco, José
last_name: Ruiz-Franco
- first_name: Virginie
full_name: Hugouvieux, Virginie
last_name: Hugouvieux
- first_name: Emanuela
full_name: Zaccarelli, Emanuela
last_name: Zaccarelli
- first_name: Cristian
full_name: Micheletti, Cristian
last_name: Micheletti
- first_name: Walter
full_name: Kob, Walter
last_name: Kob
- first_name: Lorenzo
full_name: Rovigatti, Lorenzo
last_name: Rovigatti
citation:
ama: Sorichetti V, Ninarello A, Ruiz-Franco J, et al. Structure and elasticity of
model disordered, polydisperse, and defect-free polymer networks. Journal of
Chemical Physics. 2023;158(7). doi:10.1063/5.0134271
apa: Sorichetti, V., Ninarello, A., Ruiz-Franco, J., Hugouvieux, V., Zaccarelli,
E., Micheletti, C., … Rovigatti, L. (2023). Structure and elasticity of model
disordered, polydisperse, and defect-free polymer networks. Journal of Chemical
Physics. American Institute of Physics. https://doi.org/10.1063/5.0134271
chicago: Sorichetti, Valerio, Andrea Ninarello, José Ruiz-Franco, Virginie Hugouvieux,
Emanuela Zaccarelli, Cristian Micheletti, Walter Kob, and Lorenzo Rovigatti. “Structure
and Elasticity of Model Disordered, Polydisperse, and Defect-Free Polymer Networks.”
Journal of Chemical Physics. American Institute of Physics, 2023. https://doi.org/10.1063/5.0134271.
ieee: V. Sorichetti et al., “Structure and elasticity of model disordered,
polydisperse, and defect-free polymer networks,” Journal of Chemical Physics,
vol. 158, no. 7. American Institute of Physics, 2023.
ista: Sorichetti V, Ninarello A, Ruiz-Franco J, Hugouvieux V, Zaccarelli E, Micheletti
C, Kob W, Rovigatti L. 2023. Structure and elasticity of model disordered, polydisperse,
and defect-free polymer networks. Journal of Chemical Physics. 158(7), 074905.
mla: Sorichetti, Valerio, et al. “Structure and Elasticity of Model Disordered,
Polydisperse, and Defect-Free Polymer Networks.” Journal of Chemical Physics,
vol. 158, no. 7, 074905, American Institute of Physics, 2023, doi:10.1063/5.0134271.
short: V. Sorichetti, A. Ninarello, J. Ruiz-Franco, V. Hugouvieux, E. Zaccarelli,
C. Micheletti, W. Kob, L. Rovigatti, Journal of Chemical Physics 158 (2023).
date_created: 2023-03-05T23:01:05Z
date_published: 2023-02-21T00:00:00Z
date_updated: 2023-10-03T11:31:51Z
day: '21'
department:
- _id: AnSa
doi: 10.1063/5.0134271
external_id:
arxiv:
- '2211.04810'
isi:
- '000936943800002'
pmid:
- '36813705'
intvolume: ' 158'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.2211.04810
month: '02'
oa: 1
oa_version: Preprint
pmid: 1
publication: Journal of Chemical Physics
publication_identifier:
eissn:
- 1089-7690
issn:
- 0021-9606
publication_status: published
publisher: American Institute of Physics
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structure and elasticity of model disordered, polydisperse, and defect-free
polymer networks
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 158
year: '2023'
...
---
_id: '14655'
abstract:
- lang: eng
text: The kinetics of the assembly of semiflexible filaments through end-to-end
annealing is key to the structure of the cytoskeleton, but is not understood.
We analyze this problem through scaling theory and simulations, and uncover a
regime where filaments’ ends find each other through bending fluctuations without
the need for the whole filament to diffuse. This results in a very substantial
speedup of assembly in physiological regimes, and could help with understanding
the dynamics of actin and intermediate filaments in biological processes such
as wound healing and cell division.
acknowledgement: The authors thank C´ecile Leduc and Duc-Quang Tran for invaluable
help with understanding the experimental behavior of intermediate filaments, and
Raphael Voituriez, Nicolas Levernier, and Alexander Grosberg for fruitful discussion
on the theoretical model. V. S. also thanks Davide Michieletto, Maria Panoukidou,
and Lorenzo Rovigatti for very helpful suggestions on the simulation model. M. L.
was supported by Marie Curie Integration Grant No. PCIG12-GA-2012-334053, “Investissements
d’Avenir” LabEx PALM (ANR-10-LABX- 0039-PALM), ANR Grants No. ANR-15-CE13-0004-03,
No. ANR-21-CE11-0004-02 and No. ANR-22-CE30-0024, as well as ERC Starting Grant
No. 677532. M.L.’s group belongs to the CNRS consortium AQV. Part of this work was
performed using HPC resources from GENCI–IDRIS (Grants No. 2020-A0090712066 and
No. 2021-A0110712066).
article_number: '228401'
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Valerio
full_name: Sorichetti, Valerio
id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
last_name: Sorichetti
orcid: 0000-0002-9645-6576
- first_name: Martin
full_name: Lenz, Martin
last_name: Lenz
citation:
ama: Sorichetti V, Lenz M. Transverse fluctuations control the assembly of semiflexible
filaments. Physical Review Letters. 2023;131(22). doi:10.1103/PhysRevLett.131.228401
apa: Sorichetti, V., & Lenz, M. (2023). Transverse fluctuations control the
assembly of semiflexible filaments. Physical Review Letters. American Physical
Society. https://doi.org/10.1103/PhysRevLett.131.228401
chicago: Sorichetti, Valerio, and Martin Lenz. “Transverse Fluctuations Control
the Assembly of Semiflexible Filaments.” Physical Review Letters. American
Physical Society, 2023. https://doi.org/10.1103/PhysRevLett.131.228401.
ieee: V. Sorichetti and M. Lenz, “Transverse fluctuations control the assembly of
semiflexible filaments,” Physical Review Letters, vol. 131, no. 22. American
Physical Society, 2023.
ista: Sorichetti V, Lenz M. 2023. Transverse fluctuations control the assembly of
semiflexible filaments. Physical Review Letters. 131(22), 228401.
mla: Sorichetti, Valerio, and Martin Lenz. “Transverse Fluctuations Control the
Assembly of Semiflexible Filaments.” Physical Review Letters, vol. 131,
no. 22, 228401, American Physical Society, 2023, doi:10.1103/PhysRevLett.131.228401.
short: V. Sorichetti, M. Lenz, Physical Review Letters 131 (2023).
corr_author: '1'
date_created: 2023-12-10T23:00:57Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2025-09-09T13:35:06Z
day: '01'
department:
- _id: AnSa
doi: 10.1103/PhysRevLett.131.228401
external_id:
arxiv:
- '2303.03088'
isi:
- '001163810200008'
pmid:
- '38101392'
intvolume: ' 131'
isi: 1
issue: '22'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.48550/arXiv.2303.03088
month: '12'
oa: 1
oa_version: Preprint
pmid: 1
publication: Physical Review Letters
publication_identifier:
eissn:
- 1079-7114
issn:
- 0031-9007
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transverse fluctuations control the assembly of semiflexible filaments
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 131
year: '2023'
...