---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21483'
abstract:
- lang: eng
  text: 'Embryogenesis in the model plant Arabidopsis thaliana provides a framework
    for understanding how cell polarity and patterning coordinate with hormonal signalling
    to establish the plant body plan. Following fertilisation, the zygote divides
    asymmetrically to generate apical and basal lineages, establishing the apical–basal
    axis that defines future shoot and root poles. Genetic and molecular analyses
    of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless
    revealed that localised auxin biosynthesis, directional transport and downstream
    transcriptional responses are central to apical–basal axis establishment and organ
    initiation. The main components of this regulation are polarly localised PIN auxin
    transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX
    transcription factors. Advances in microscopy have transformed the study of Arabidopsis
    embryogenesis: fluorescence-compatible clearing reagents and three-dimensional
    reconstructions now permit quantitative analyses of cell geometry, division orientation,
    and cytoskeletal dynamics. Live ovule imaging setups with confocal laser scanning
    and multiphoton microscopes enable real-time observation of embryo development,
    while laser-assisted cell ablation can be used to probe cell-to-cell communication
    and fate plasticity. Together, these methodological breakthroughs position Arabidopsis
    embryos as a prime model for dissecting the chemical and biophysical cues that
    shape plant development.'
acknowledgement: The authors would like to acknowledge the many colleagues whose valuable
  contributions to the field could not be included in this review due to space limitations
  and reference constraints. Open Access funding provided by Institute of Science
  and Technology Austria/KEMÖ.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: David
  full_name: Babic, David
  id: db566d23-f6e0-11ea-865d-e6f270e968e7
  last_name: Babic
- first_name: Milan
  full_name: Zupunski, Milan
  id: f6a21fce-573e-11f0-a150-a8d96aee2539
  last_name: Zupunski
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Babic D, Zupunski M, Friml J. Imaging and genetic toolbox to study Arabidopsis
    embryogenesis. <i>New Phytologist</i>. 2026;250(3):1483-1491. doi:<a href="https://doi.org/10.1111/nph.71072">10.1111/nph.71072</a>
  apa: Babic, D., Zupunski, M., &#38; Friml, J. (2026). Imaging and genetic toolbox
    to study Arabidopsis embryogenesis. <i>New Phytologist</i>. Wiley. <a href="https://doi.org/10.1111/nph.71072">https://doi.org/10.1111/nph.71072</a>
  chicago: Babic, David, Milan Zupunski, and Jiří Friml. “Imaging and Genetic Toolbox
    to Study Arabidopsis Embryogenesis.” <i>New Phytologist</i>. Wiley, 2026. <a href="https://doi.org/10.1111/nph.71072">https://doi.org/10.1111/nph.71072</a>.
  ieee: D. Babic, M. Zupunski, and J. Friml, “Imaging and genetic toolbox to study
    Arabidopsis embryogenesis,” <i>New Phytologist</i>, vol. 250, no. 3. Wiley, pp.
    1483–1491, 2026.
  ista: Babic D, Zupunski M, Friml J. 2026. Imaging and genetic toolbox to study Arabidopsis
    embryogenesis. New Phytologist. 250(3), 1483–1491.
  mla: Babic, David, et al. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.”
    <i>New Phytologist</i>, vol. 250, no. 3, Wiley, 2026, pp. 1483–91, doi:<a href="https://doi.org/10.1111/nph.71072">10.1111/nph.71072</a>.
  short: D. Babic, M. Zupunski, J. Friml, New Phytologist 250 (2026) 1483–1491.
corr_author: '1'
das_tickbox: '0'
date_created: 2026-03-23T14:59:06Z
date_published: 2026-05-01T00:00:00Z
date_updated: 2026-07-27T11:47:24Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: GradSch
doi: 10.1111/nph.71072
external_id:
  pmid:
  - '41808651'
file:
- access_level: open_access
  checksum: 67513dde983631bed9613fdaadbbee06
  content_type: application/pdf
  creator: dernst
  date_created: 2026-07-27T11:46:25Z
  date_updated: 2026-07-27T11:46:25Z
  file_id: '22424'
  file_name: 2026_NewPhytologist_Babic.pdf
  file_size: 1708392
  relation: main_file
  success: 1
file_date_updated: 2026-07-27T11:46:25Z
fulldoi: https://doi.org/10.1111/nph.71072
has_accepted_license: '1'
intvolume: '       250'
issue: '3'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1483-1491
pmid: 1
publication: New Phytologist
publication_identifier:
  eissn:
  - 1469-8137
  issn:
  - 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: Imaging and genetic toolbox to study Arabidopsis embryogenesis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 250
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '22927'
abstract:
- lang: eng
  text: Auxin and calcium (Ca2+) are central plant signals that coordinate growth,
    development, and responses to environmental cues. Typically studied as largely
    parallel pathways, they are now increasingly recognized as interconnected networks
    whose activities converge at multiple regulatory levels. Recent advances in live
    imaging, biosensors, optogenetics, and structural approaches have revealed that
    auxin can rapidly trigger Ca2+ influx from the apoplast via TRANSPORT INHIBITOR
    RESPONSE1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB)-dependent signaling mechanisms and
    Ca2+ channels, presumably involving cyclic nucleotides produced by TIR1/AFB auxin
    receptors. Conversely, Ca2+ signals arising from mechanosensing, wounding, and
    environmental stress can modulate auxin transport, signaling, and transcriptional
    outputs. Together, these discoveries highlight a dynamic, reciprocal signaling
    axis where Ca2+ acts both downstream and upstream of auxin to shape growth, development,
    tropisms, and regeneration.
acknowledgement: The authors would like to acknowledge many colleagues whose valuable
  contributions to the field could not be included in this review due to space limitations
  and reference constraints.
article_processing_charge: No
article_type: original
author:
- first_name: Milan
  full_name: Zupunski, Milan
  id: f6a21fce-573e-11f0-a150-a8d96aee2539
  last_name: Zupunski
- first_name: Guido
  full_name: Grossmann, Guido
  last_name: Grossmann
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: 'Zupunski M, Grossmann G, Friml J. Decoding dialog: The auxin–calcium signaling
    crosstalk in plants. <i>Trends in Plant Science</i>. 2026. doi:<a href="https://doi.org/10.1016/j.tplants.2026.08.002">10.1016/j.tplants.2026.08.002</a>'
  apa: 'Zupunski, M., Grossmann, G., &#38; Friml, J. (2026). Decoding dialog: The
    auxin–calcium signaling crosstalk in plants. <i>Trends in Plant Science</i>. Cell
    Press. <a href="https://doi.org/10.1016/j.tplants.2026.08.002">https://doi.org/10.1016/j.tplants.2026.08.002</a>'
  chicago: 'Zupunski, Milan, Guido Grossmann, and Jiří Friml. “Decoding Dialog: The
    Auxin–Calcium Signaling Crosstalk in Plants.” <i>Trends in Plant Science</i>.
    Cell Press, 2026. <a href="https://doi.org/10.1016/j.tplants.2026.08.002">https://doi.org/10.1016/j.tplants.2026.08.002</a>.'
  ieee: 'M. Zupunski, G. Grossmann, and J. Friml, “Decoding dialog: The auxin–calcium
    signaling crosstalk in plants,” <i>Trends in Plant Science</i>. Cell Press, 2026.'
  ista: 'Zupunski M, Grossmann G, Friml J. 2026. Decoding dialog: The auxin–calcium
    signaling crosstalk in plants. Trends in Plant Science.'
  mla: 'Zupunski, Milan, et al. “Decoding Dialog: The Auxin–Calcium Signaling Crosstalk
    in Plants.” <i>Trends in Plant Science</i>, Cell Press, 2026, doi:<a href="https://doi.org/10.1016/j.tplants.2026.08.002">10.1016/j.tplants.2026.08.002</a>.'
  short: M. Zupunski, G. Grossmann, J. Friml, Trends in Plant Science (2026).
corr_author: '1'
das_tickbox: '0'
date_created: 2026-09-13T22:01:56Z
date_published: 2026-09-04T00:00:00Z
date_updated: 2026-09-17T07:42:27Z
day: '04'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2026.08.002
external_id:
  pmid:
  - '42692943'
fulldoi: https://doi.org/10.1016/j.tplants.2026.08.002
has_accepted_license: '1'
keyword:
- auxin
- calcium
- rapid signaling
- crosstalk
- growth regulation
- cyclic nucleotides
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.tplants.2026.08.002
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Trends in Plant Science
publication_identifier:
  eissn:
  - 1878-4372
  issn:
  - 1360-1385
publication_status: epub_ahead
publisher: Cell Press
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: 'Decoding dialog: The auxin–calcium signaling crosstalk in plants'
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...