[{"pmid":1,"type":"journal_article","quality_controlled":"1","project":[{"call_identifier":"H2020","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","grant_number":"802960"},{"name":"EMBO Young Investigator Program - Andela Saric","_id":"349b6ff1-11ca-11ed-8bc3-f006047c2eeb"}],"OA_type":"hybrid","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","citation":{"apa":"Parham, J., Sorichetti, V., Cezanne, A., Foo, S., Kuo, Y. W., Hoogenberg, B., … Baum, B. (2025). Temporal and spatial coordination of DNA segregation and cell division in an archaeon. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2513939122\">https://doi.org/10.1073/pnas.2513939122</a>","ieee":"J. Parham <i>et al.</i>, “Temporal and spatial coordination of DNA segregation and cell division in an archaeon,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 42. National Academy of Sciences, p. e2513939122, 2025.","chicago":"Parham, Joe, Valerio Sorichetti, Alice Cezanne, Sherman Foo, Yin Wei Kuo, Baukje Hoogenberg, Arthur Radoux-Mergault, et al. “Temporal and Spatial Coordination of DNA Segregation and Cell Division in an Archaeon.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2513939122\">https://doi.org/10.1073/pnas.2513939122</a>.","short":"J. Parham, V. Sorichetti, A. Cezanne, S. Foo, Y.W. Kuo, B. Hoogenberg, A. Radoux-Mergault, E. Mawdesley, L.D. Gatward, J. Boulanger, U. Schulze, A. Šarić, B. Baum, Proceedings of the National Academy of Sciences 122 (2025) e2513939122.","ama":"Parham J, Sorichetti V, Cezanne A, et al. Temporal and spatial coordination of DNA segregation and cell division in an archaeon. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(42):e2513939122. doi:<a href=\"https://doi.org/10.1073/pnas.2513939122\">10.1073/pnas.2513939122</a>","ista":"Parham J, Sorichetti V, Cezanne A, Foo S, Kuo YW, Hoogenberg B, Radoux-Mergault A, Mawdesley E, Gatward LD, Boulanger J, Schulze U, Šarić A, Baum B. 2025. Temporal and spatial coordination of DNA segregation and cell division in an archaeon. Proceedings of the National Academy of Sciences. 122(42), e2513939122.","mla":"Parham, Joe, et al. “Temporal and Spatial Coordination of DNA Segregation and Cell Division in an Archaeon.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 42, National Academy of Sciences, 2025, p. e2513939122, doi:<a href=\"https://doi.org/10.1073/pnas.2513939122\">10.1073/pnas.2513939122</a>."},"issue":"42","PlanS_conform":"1","publication_identifier":{"eissn":["1091-6490"]},"has_accepted_license":"1","acknowledgement":"We thank Matthew Kenneth for his assistance with live cell imaging. We thank Arthur Charles-Orszag and Dyche Mullins for generously gifting the SegA and SegB antibodies, and Sonja-Verena Albers for gifting the CdvA-HA overexpression plasmid. We thank the Light Microscopy and Flow Cytometry facilities at the MRC-LMB, and all the core staff at the MRC-LMB for their support. We thank all members of the Baum lab for helpful discussions. We would like to thank Magdalena Lechowska, Gautam Dey, Laura Downie, and Iva Tolic for critical reading of the manuscript. J.P. was supported by the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27). A.C. was funded by an EMBO Postdoctoral fellowship (ALTF_1041-2021), a Marie Sklodowska-Curie Individual Fellowship (101068523) provided by UKRI and by the Wellcome Trust (222460/Z/21/Z). B.H. was supported by Wellcome Trust (203276/A/16/Z). Y.-W.K. was supported by an EMBO postdoctoral fellowship (ALTF 903-2021) and by the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27); S.F. was supported by the Wellcome Trust (222460/Z/21/Z); B.B. received support from the MRC LMB, the Wellcome Trust (203276/Z/16/Z) and (222460/Z/21/Z), the VW Foundation (94933), and from the Gordon and Betty Moore Foundation’s Symbiosis in Aquatic Systems Initiative (9346). V.S. and A.Š. acknowledge funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (grant no.802960 to A.Š.), the Vallee Scholarship, and the EMBO Young Investigator Programme (A.Š.). The collaborative work of A.Š.’s and B.B. teams was also supported by a Moore–Simons Project on the Origin of the Eukaryotic Cell, Simons Foundation 735929LPI.","file_date_updated":"2025-10-27T08:12:59Z","volume":122,"license":"https://creativecommons.org/licenses/by/4.0/","file":[{"content_type":"application/pdf","checksum":"3555d51f438d2e356039a9b697eac3ee","file_name":"2025_PNAS_Parham.pdf","success":1,"creator":"dernst","file_id":"20543","relation":"main_file","access_level":"open_access","date_updated":"2025-10-27T08:12:59Z","file_size":2649194,"date_created":"2025-10-27T08:12:59Z"}],"status":"public","ec_funded":1,"_id":"20530","author":[{"full_name":"Parham, Joe","last_name":"Parham","first_name":"Joe"},{"last_name":"Sorichetti","full_name":"Sorichetti, Valerio","orcid":"0000-0002-9645-6576","id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b","first_name":"Valerio"},{"full_name":"Cezanne, Alice","last_name":"Cezanne","first_name":"Alice"},{"last_name":"Foo","full_name":"Foo, Sherman","first_name":"Sherman"},{"full_name":"Kuo, Yin Wei","last_name":"Kuo","first_name":"Yin Wei"},{"first_name":"Baukje","full_name":"Hoogenberg, Baukje","last_name":"Hoogenberg"},{"full_name":"Radoux-Mergault, Arthur","last_name":"Radoux-Mergault","first_name":"Arthur"},{"first_name":"Eloise","full_name":"Mawdesley, Eloise","last_name":"Mawdesley"},{"last_name":"Gatward","full_name":"Gatward, Lydia Daniels","first_name":"Lydia Daniels"},{"first_name":"Jerome","last_name":"Boulanger","full_name":"Boulanger, Jerome"},{"last_name":"Schulze","full_name":"Schulze, Ulrike","first_name":"Ulrike"},{"last_name":"Šarić","full_name":"Šarić, Anđela","orcid":"0000-0002-7854-2139","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","first_name":"Anđela"},{"full_name":"Baum, Buzz","last_name":"Baum","first_name":"Buzz"}],"article_processing_charge":"Yes (in subscription journal)","publisher":"National Academy of Sciences","day":"21","year":"2025","date_published":"2025-10-21T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"AnSa"}],"oa":1,"intvolume":"       122","isi":1,"ddc":["570"],"month":"10","publication":"Proceedings of the National Academy of Sciences","page":"e2513939122","date_created":"2025-10-26T23:01:33Z","doi":"10.1073/pnas.2513939122","OA_place":"publisher","external_id":{"pmid":["41091768"],"isi":["001620648600001"]},"title":"Temporal and spatial coordination of DNA segregation and cell division in an archaeon","abstract":[{"lang":"eng","text":"Cells must coordinate DNA segregation with cytokinesis to ensure that each daughter cell inherits a complete genome. Here, we explore how DNA segregation and division are mechanistically coupled in archaeal relatives of eukaryotes, which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we first describe the series of sequential changes in DNA organization that accompany cell division in Sulfolobus, which computational modeling shows likely aid genome segregation. Through a perturbation analysis we identify a regulatory checkpoint which ensures that the compaction of the genome into two spatially segregated nucleoids only occurs once cells have assembled a division ring—which also defines the axis of DNA segregation. Finally, we show that DNA compaction and segregation depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence leads to bridging DNA. Taken together, these data show how regulatory checkpoints like those operating in eukaryotes aid high-fidelity division in an archaeon."}],"publication_status":"published","date_updated":"2026-02-16T12:32:31Z","scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}]},{"date_created":"2026-02-16T14:50:32Z","publication":"PRX Life","corr_author":"1","month":"08","ddc":["570"],"intvolume":"         3","oa":1,"language":[{"iso":"eng"}],"oa_version":"Published Version","publication_status":"published","date_updated":"2026-02-17T11:16:26Z","DOAJ_listed":"1","title":"Charge distribution of the coating brush drives interchromosome attraction","abstract":[{"text":"The condensation of charged polymers is an important driver for the formation of biomolecular condensates. Recent experiments suggest that this mechanism also controls the clustering of eukaryotic chromosomes during the late stages of cell division. In this process, interchromosome attraction is driven by the condensation of cytoplasmic RNA and Ki-67, a charged intrinsically disordered protein that coats the chromosomes as a brush. Attraction between chromosomes has been shown to be specifically promoted by a localized charged patch on Ki-67, although the physical mechanism remains unclear. To elucidate this process, we combine coarse-grained simulations and analytical theory to study the RNA-mediated interaction between charged polymer brushes on the chromosome surfaces. We show that the charged patch on Ki-67 leads to interchromosome attraction via RNA bridging between the two brushes, whereby the RNA preferentially interacts with the charged patches, leading to stable, long-range forces. By contrast, if the brush is uniformly charged, bridging is basically absent due to complete adsorption of RNA onto the brush. Moreover, the RNA dynamics becomes caged in presence of the charged patch while remaining diffusive with uniform charge. Our work sheds light on the physical origin of chromosome clustering, while also suggesting a general mechanism for cells to tune work production by biomolecular condensates via different charge distributions.","lang":"eng"}],"OA_place":"publisher","doi":"10.1103/41fd-r847","PlanS_conform":"1","has_accepted_license":"1","publication_identifier":{"eissn":["2835-8279"]},"article_number":"033010","issue":"3","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","OA_type":"gold","citation":{"ista":"Sorichetti V, Robin P, Palaia I, Hernandez-Armendariz A, Cuylen-Haering S, Šarić A. 2025. Charge distribution of the coating brush drives interchromosome attraction. PRX Life. 3(3), 033010.","ama":"Sorichetti V, Robin P, Palaia I, Hernandez-Armendariz A, Cuylen-Haering S, Šarić A. Charge distribution of the coating brush drives interchromosome attraction. <i>PRX Life</i>. 2025;3(3). doi:<a href=\"https://doi.org/10.1103/41fd-r847\">10.1103/41fd-r847</a>","apa":"Sorichetti, V., Robin, P., Palaia, I., Hernandez-Armendariz, A., Cuylen-Haering, S., &#38; Šarić, A. (2025). Charge distribution of the coating brush drives interchromosome attraction. <i>PRX Life</i>. American Physical Society. <a href=\"https://doi.org/10.1103/41fd-r847\">https://doi.org/10.1103/41fd-r847</a>","ieee":"V. Sorichetti, P. Robin, I. Palaia, A. Hernandez-Armendariz, S. Cuylen-Haering, and A. Šarić, “Charge distribution of the coating brush drives interchromosome attraction,” <i>PRX Life</i>, vol. 3, no. 3. American Physical Society, 2025.","chicago":"Sorichetti, Valerio, Paul Robin, Ivan Palaia, Alberto Hernandez-Armendariz, Sara Cuylen-Haering, and Anđela Šarić. “Charge Distribution of the Coating Brush Drives Interchromosome Attraction.” <i>PRX Life</i>. American Physical Society, 2025. <a href=\"https://doi.org/10.1103/41fd-r847\">https://doi.org/10.1103/41fd-r847</a>.","short":"V. Sorichetti, P. Robin, I. Palaia, A. Hernandez-Armendariz, S. Cuylen-Haering, A. Šarić, PRX Life 3 (2025).","mla":"Sorichetti, Valerio, et al. “Charge Distribution of the Coating Brush Drives Interchromosome Attraction.” <i>PRX Life</i>, vol. 3, no. 3, 033010, American Physical Society, 2025, doi:<a href=\"https://doi.org/10.1103/41fd-r847\">10.1103/41fd-r847</a>."},"quality_controlled":"1","project":[{"call_identifier":"H2020","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","grant_number":"802960"},{"name":"IST-BRIDGE: International postdoctoral program","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","call_identifier":"H2020","grant_number":"101034413"},{"_id":"349b6ff1-11ca-11ed-8bc3-f006047c2eeb","name":"EMBO Young Investigator Program - Andela Saric"}],"type":"journal_article","date_published":"2025-08-11T00:00:00Z","year":"2025","day":"11","department":[{"_id":"AnSa"},{"_id":"EdHa"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b","first_name":"Valerio","orcid":"0000-0002-9645-6576","full_name":"Sorichetti, Valerio","last_name":"Sorichetti"},{"first_name":"Paul","id":"48c58128-57b0-11ee-9095-dc28fd97fc1d","orcid":"0000-0002-5728-9189","full_name":"Robin, Paul","last_name":"Robin"},{"last_name":"Palaia","full_name":"Palaia, Ivan","orcid":" 0000-0002-8843-9485 ","id":"9c805cd2-4b75-11ec-a374-db6dd0ed57fa","first_name":"Ivan"},{"last_name":"Hernandez-Armendariz","full_name":"Hernandez-Armendariz, Alberto","first_name":"Alberto"},{"first_name":"Sara","full_name":"Cuylen-Haering, Sara","last_name":"Cuylen-Haering"},{"orcid":"0000-0002-7854-2139","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","first_name":"Anđela","last_name":"Šarić","full_name":"Šarić, Anđela"}],"_id":"21235","article_processing_charge":"Yes","publisher":"American Physical Society","file_date_updated":"2026-02-17T11:12:30Z","volume":3,"ec_funded":1,"status":"public","file":[{"date_created":"2026-02-17T11:12:30Z","file_size":3732843,"access_level":"open_access","date_updated":"2026-02-17T11:12:30Z","file_id":"21287","relation":"main_file","content_type":"application/pdf","file_name":"2025_PRXLife_Sorichetti.pdf","checksum":"1702b9bdbfd902a7c08aa4f1479b390d","creator":"dernst","success":1}],"acknowledgement":"This work was supported by the European Union’s Horizon 2020 research and innovation programme (A.Š. and V.S., ERC grant Agreement No. 802960 to A.Š., I.P. and P.R.,\r\nMarie Skłodowska-Curie Grant Agreement No. 101034413), the German Research Foundation (S.C-H. and A.H.-A., DFG Project No. 402723784 to S.C-H.), the Vallee Scholarship\r\n(A.Š. and V.S.), the EMBO Young Investigator Programme (A.Š.), and a Ph.D. fellowship from the Boehringer Ingelheim Fonds (A.H.-A.)."},{"publication":"PRX Life","date_created":"2026-02-16T15:55:03Z","ddc":["570"],"month":"09","corr_author":"1","intvolume":"         3","oa":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"date_updated":"2026-02-17T13:37:38Z","publication_status":"published","abstract":[{"text":"Collagen IV is one of the main components of the basement membrane, a layer of material that lines the majority of tissues in multicellular organisms. Collagen IV molecules assemble into networks, providing stiffness and elasticity to tissues and informing cell and organ shape, especially during development. In this work, we develop two coarse-grained models for collagen IV molecules that retain biochemical bond specificity and coarse grain at different length scales. Through molecular-dynamics simulations, we test the assembly and mechanics of the resulting networks and measure their response to strain in terms of stress, microscopic alignment, and bond dynamics. Within the basement membrane, collagen IV networks rearrange by molecule turnover, which affects tissue organization and can be linked with enzyme activity. Here we explore network rearrangements via bond remodeling, the process of breaking and remaking of bonds between network molecules. We then investigate the effects of active (enzymatic) bond remodeling. We find that this nonequilibrium remodeling allows a network to keep its integrity under strain, while relaxing fully over a variety of timescales, a dynamic response that is unavailable to networks undergoing equilibrium remodeling.","lang":"eng"}],"title":"Nonequilibrium remodeling of collagen IV networks in Silico","DOAJ_listed":"1","doi":"10.1103/gdd5-rnh7","OA_place":"publisher","publication_identifier":{"eissn":["2835-8279"]},"has_accepted_license":"1","PlanS_conform":"1","article_number":"033019","citation":{"apa":"Meadowcroft, B., Sorichetti, V., Ratajczyk, E., Perez Verdugo, F. L., Khalilgharibi, N., Mao, Y., … Šarić, A. (2025). Nonequilibrium remodeling of collagen IV networks in Silico. <i>PRX Life</i>. American Physical Society. <a href=\"https://doi.org/10.1103/gdd5-rnh7\">https://doi.org/10.1103/gdd5-rnh7</a>","ieee":"B. Meadowcroft <i>et al.</i>, “Nonequilibrium remodeling of collagen IV networks in Silico,” <i>PRX Life</i>, vol. 3. American Physical Society, 2025.","chicago":"Meadowcroft, Billie, Valerio Sorichetti, Eryk Ratajczyk, Fernanda L Perez Verdugo, Nargess Khalilgharibi, Yanlan Mao, Ivan Palaia, and Anđela Šarić. “Nonequilibrium Remodeling of Collagen IV Networks in Silico.” <i>PRX Life</i>. American Physical Society, 2025. <a href=\"https://doi.org/10.1103/gdd5-rnh7\">https://doi.org/10.1103/gdd5-rnh7</a>.","short":"B. Meadowcroft, V. Sorichetti, E. Ratajczyk, F.L. Perez Verdugo, N. Khalilgharibi, Y. Mao, I. Palaia, A. Šarić, PRX Life 3 (2025).","ista":"Meadowcroft B, Sorichetti V, Ratajczyk E, Perez Verdugo FL, Khalilgharibi N, Mao Y, Palaia I, Šarić A. 2025. Nonequilibrium remodeling of collagen IV networks in Silico. PRX Life. 3, 033019.","ama":"Meadowcroft B, Sorichetti V, Ratajczyk E, et al. Nonequilibrium remodeling of collagen IV networks in Silico. <i>PRX Life</i>. 2025;3. doi:<a href=\"https://doi.org/10.1103/gdd5-rnh7\">10.1103/gdd5-rnh7</a>","mla":"Meadowcroft, Billie, et al. “Nonequilibrium Remodeling of Collagen IV Networks in Silico.” <i>PRX Life</i>, vol. 3, 033019, American Physical Society, 2025, doi:<a href=\"https://doi.org/10.1103/gdd5-rnh7\">10.1103/gdd5-rnh7</a>."},"OA_type":"gold","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","type":"journal_article","project":[{"name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","call_identifier":"H2020","grant_number":"802960"},{"grant_number":"101034413","call_identifier":"H2020","name":"IST-BRIDGE: International postdoctoral program","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c"},{"_id":"349b6ff1-11ca-11ed-8bc3-f006047c2eeb","name":"EMBO Young Investigator Program - Andela Saric"}],"quality_controlled":"1","department":[{"_id":"AnSa"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"05","year":"2025","date_published":"2025-09-05T00:00:00Z","publisher":"American Physical Society","_id":"21256","author":[{"orcid":"0000-0003-3441-1337","id":"a4725fd6-932b-11ed-81e2-c098c7f37ae1","first_name":"Billie","last_name":"Meadowcroft","full_name":"Meadowcroft, Billie"},{"full_name":"Sorichetti, Valerio","last_name":"Sorichetti","id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b","first_name":"Valerio","orcid":"0000-0002-9645-6576"},{"first_name":"Eryk","last_name":"Ratajczyk","full_name":"Ratajczyk, Eryk"},{"id":"4ecec223-9070-11ef-a0a9-bc76077bea8d","first_name":"Fernanda L","last_name":"Perez Verdugo","full_name":"Perez Verdugo, Fernanda L"},{"first_name":"Nargess","last_name":"Khalilgharibi","full_name":"Khalilgharibi, Nargess"},{"full_name":"Mao, Yanlan","last_name":"Mao","first_name":"Yanlan"},{"full_name":"Palaia, Ivan","last_name":"Palaia","id":"9c805cd2-4b75-11ec-a374-db6dd0ed57fa","first_name":"Ivan","orcid":" 0000-0002-8843-9485 "},{"first_name":"Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","orcid":"0000-0002-7854-2139","full_name":"Šarić, Anđela","last_name":"Šarić"}],"article_processing_charge":"Yes","file":[{"access_level":"open_access","date_updated":"2026-02-17T13:36:01Z","date_created":"2026-02-17T13:36:01Z","file_size":2277704,"content_type":"application/pdf","file_name":"2025_PRXLife_Meadowcroft.pdf","checksum":"04cae5231d97e533145c493880fadbd9","success":1,"creator":"dernst","file_id":"21308","relation":"main_file"}],"status":"public","ec_funded":1,"file_date_updated":"2026-02-17T13:36:01Z","volume":3,"acknowledgement":"This work received funding from the European Research Council under the European Union's Horizon 2020 research and innovation program through Grant Agreement No. 802960 (B.M., V.S., I.P., and A.Š.), the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 101034413 (I.P.), the NOMIS Foundation (F.P.-V.), the National Centre for the Replacement, Refinement and Reduction of Animals in Research Grant No. NC/T002425/1 (N.K.), Leverhulme Trust project Grant No. RPG-2020-068 (N.K.), MRC Fellowship No. MR/W027437/1 (Y.M.), a Lister Institute Research Prize (Y.M.) and EMBO Young Investigator Programme (Y.M. and A.Š.)."}]