---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20530'
abstract:
- lang: eng
  text: Cells must coordinate DNA segregation with cytokinesis to ensure that each
    daughter cell inherits a complete genome. Here, we explore how DNA segregation
    and division are mechanistically coupled in archaeal relatives of eukaryotes,
    which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we
    first describe the series of sequential changes in DNA organization that accompany
    cell division in Sulfolobus, which computational modeling shows likely aid genome
    segregation. Through a perturbation analysis we identify a regulatory checkpoint
    which ensures that the compaction of the genome into two spatially segregated
    nucleoids only occurs once cells have assembled a division ring—which also defines
    the axis of DNA segregation. Finally, we show that DNA compaction and segregation
    depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence
    leads to bridging DNA. Taken together, these data show how regulatory checkpoints
    like those operating in eukaryotes aid high-fidelity division in an archaeon.
acknowledgement: We thank Matthew Kenneth for his assistance with live cell imaging.
  We thank Arthur Charles-Orszag and Dyche Mullins for generously gifting the SegA
  and SegB antibodies, and Sonja-Verena Albers for gifting the CdvA-HA overexpression
  plasmid. We thank the Light Microscopy and Flow Cytometry facilities at the MRC-LMB,
  and all the core staff at the MRC-LMB for their support. We thank all members of
  the Baum lab for helpful discussions. We would like to thank Magdalena Lechowska,
  Gautam Dey, Laura Downie, and Iva Tolic for critical reading of the manuscript.
  J.P. was supported by the Medical Research Council—Laboratory of Molecular Biology
  (MC_UP_1201/27). A.C. was funded by an EMBO Postdoctoral fellowship (ALTF_1041-2021),
  a Marie Sklodowska-Curie Individual Fellowship (101068523) provided by UKRI and
  by the Wellcome Trust (222460/Z/21/Z). B.H. was supported by Wellcome Trust (203276/A/16/Z).
  Y.-W.K. was supported by an EMBO postdoctoral fellowship (ALTF 903-2021) and by
  the Medical Research Council—Laboratory of Molecular Biology (MC_UP_1201/27); S.F.
  was supported by the Wellcome Trust (222460/Z/21/Z); B.B. received support from
  the MRC LMB, the Wellcome Trust (203276/Z/16/Z) and (222460/Z/21/Z), the VW Foundation
  (94933), and from the Gordon and Betty Moore Foundation’s Symbiosis in Aquatic Systems
  Initiative (9346). V.S. and A.Š. acknowledge funding from the European Research
  Council under the European Union’s Horizon 2020 research and innovation programme
  (grant no.802960 to A.Š.), the Vallee Scholarship, and the EMBO Young Investigator
  Programme (A.Š.). The collaborative work of A.Š.’s and B.B. teams was also supported
  by a Moore–Simons Project on the Origin of the Eukaryotic Cell, Simons Foundation
  735929LPI.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Joe
  full_name: Parham, Joe
  last_name: Parham
- first_name: Valerio
  full_name: Sorichetti, Valerio
  id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
  last_name: Sorichetti
  orcid: 0000-0002-9645-6576
- first_name: Alice
  full_name: Cezanne, Alice
  last_name: Cezanne
- first_name: Sherman
  full_name: Foo, Sherman
  last_name: Foo
- first_name: Yin Wei
  full_name: Kuo, Yin Wei
  last_name: Kuo
- first_name: Baukje
  full_name: Hoogenberg, Baukje
  last_name: Hoogenberg
- first_name: Arthur
  full_name: Radoux-Mergault, Arthur
  last_name: Radoux-Mergault
- first_name: Eloise
  full_name: Mawdesley, Eloise
  last_name: Mawdesley
- first_name: Lydia Daniels
  full_name: Gatward, Lydia Daniels
  last_name: Gatward
- first_name: Jerome
  full_name: Boulanger, Jerome
  last_name: Boulanger
- first_name: Ulrike
  full_name: Schulze, Ulrike
  last_name: Schulze
- first_name: Anđela
  full_name: Šarić, Anđela
  id: bf63d406-f056-11eb-b41d-f263a6566d8b
  last_name: Šarić
  orcid: 0000-0002-7854-2139
- first_name: Buzz
  full_name: Baum, Buzz
  last_name: Baum
citation:
  ama: Parham J, Sorichetti V, Cezanne A, et al. Temporal and spatial coordination
    of DNA segregation and cell division in an archaeon. <i>Proceedings of the National
    Academy of Sciences</i>. 2025;122(42):e2513939122. doi:<a href="https://doi.org/10.1073/pnas.2513939122">10.1073/pnas.2513939122</a>
  apa: Parham, J., Sorichetti, V., Cezanne, A., Foo, S., Kuo, Y. W., Hoogenberg, B.,
    … Baum, B. (2025). Temporal and spatial coordination of DNA segregation and cell
    division in an archaeon. <i>Proceedings of the National Academy of Sciences</i>.
    National Academy of Sciences. <a href="https://doi.org/10.1073/pnas.2513939122">https://doi.org/10.1073/pnas.2513939122</a>
  chicago: Parham, Joe, Valerio Sorichetti, Alice Cezanne, Sherman Foo, Yin Wei Kuo,
    Baukje Hoogenberg, Arthur Radoux-Mergault, et al. “Temporal and Spatial Coordination
    of DNA Segregation and Cell Division in an Archaeon.” <i>Proceedings of the National
    Academy of Sciences</i>. National Academy of Sciences, 2025. <a href="https://doi.org/10.1073/pnas.2513939122">https://doi.org/10.1073/pnas.2513939122</a>.
  ieee: J. Parham <i>et al.</i>, “Temporal and spatial coordination of DNA segregation
    and cell division in an archaeon,” <i>Proceedings of the National Academy of Sciences</i>,
    vol. 122, no. 42. National Academy of Sciences, p. e2513939122, 2025.
  ista: Parham J, Sorichetti V, Cezanne A, Foo S, Kuo YW, Hoogenberg B, Radoux-Mergault
    A, Mawdesley E, Gatward LD, Boulanger J, Schulze U, Šarić A, Baum B. 2025. Temporal
    and spatial coordination of DNA segregation and cell division in an archaeon.
    Proceedings of the National Academy of Sciences. 122(42), e2513939122.
  mla: Parham, Joe, et al. “Temporal and Spatial Coordination of DNA Segregation and
    Cell Division in an Archaeon.” <i>Proceedings of the National Academy of Sciences</i>,
    vol. 122, no. 42, National Academy of Sciences, 2025, p. e2513939122, doi:<a href="https://doi.org/10.1073/pnas.2513939122">10.1073/pnas.2513939122</a>.
  short: J. Parham, V. Sorichetti, A. Cezanne, S. Foo, Y.W. Kuo, B. Hoogenberg, A.
    Radoux-Mergault, E. Mawdesley, L.D. Gatward, J. Boulanger, U. Schulze, A. Šarić,
    B. Baum, Proceedings of the National Academy of Sciences 122 (2025) e2513939122.
date_created: 2025-10-26T23:01:33Z
date_published: 2025-10-21T00:00:00Z
date_updated: 2026-02-16T12:32:31Z
day: '21'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1073/pnas.2513939122
ec_funded: 1
external_id:
  isi:
  - '001620648600001'
  pmid:
  - '41091768'
file:
- access_level: open_access
  checksum: 3555d51f438d2e356039a9b697eac3ee
  content_type: application/pdf
  creator: dernst
  date_created: 2025-10-27T08:12:59Z
  date_updated: 2025-10-27T08:12:59Z
  file_id: '20543'
  file_name: 2025_PNAS_Parham.pdf
  file_size: 2649194
  relation: main_file
  success: 1
file_date_updated: 2025-10-27T08:12:59Z
has_accepted_license: '1'
intvolume: '       122'
isi: 1
issue: '42'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: e2513939122
pmid: 1
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
  call_identifier: H2020
  grant_number: '802960'
  name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
  name: EMBO Young Investigator Program - Andela Saric
publication: Proceedings of the National Academy of Sciences
publication_identifier:
  eissn:
  - 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Temporal and spatial coordination of DNA segregation and cell division in an
  archaeon
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 122
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21235'
abstract:
- lang: eng
  text: The condensation of charged polymers is an important driver for the formation
    of biomolecular condensates. Recent experiments suggest that this mechanism also
    controls the clustering of eukaryotic chromosomes during the late stages of cell
    division. In this process, interchromosome attraction is driven by the condensation
    of cytoplasmic RNA and Ki-67, a charged intrinsically disordered protein that
    coats the chromosomes as a brush. Attraction between chromosomes has been shown
    to be specifically promoted by a localized charged patch on Ki-67, although the
    physical mechanism remains unclear. To elucidate this process, we combine coarse-grained
    simulations and analytical theory to study the RNA-mediated interaction between
    charged polymer brushes on the chromosome surfaces. We show that the charged patch
    on Ki-67 leads to interchromosome attraction via RNA bridging between the two
    brushes, whereby the RNA preferentially interacts with the charged patches, leading
    to stable, long-range forces. By contrast, if the brush is uniformly charged,
    bridging is basically absent due to complete adsorption of RNA onto the brush.
    Moreover, the RNA dynamics becomes caged in presence of the charged patch while
    remaining diffusive with uniform charge. Our work sheds light on the physical
    origin of chromosome clustering, while also suggesting a general mechanism for
    cells to tune work production by biomolecular condensates via different charge
    distributions.
acknowledgement: "This work was supported by the European Union’s Horizon 2020 research
  and innovation programme (A.Š. and V.S., ERC grant Agreement No. 802960 to A.Š.,
  I.P. and P.R.,\r\nMarie Skłodowska-Curie Grant Agreement No. 101034413), the German
  Research Foundation (S.C-H. and A.H.-A., DFG Project No. 402723784 to S.C-H.), the
  Vallee Scholarship\r\n(A.Š. and V.S.), the EMBO Young Investigator Programme (A.Š.),
  and a Ph.D. fellowship from the Boehringer Ingelheim Fonds (A.H.-A.)."
article_number: '033010'
article_processing_charge: Yes
article_type: original
author:
- first_name: Valerio
  full_name: Sorichetti, Valerio
  id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
  last_name: Sorichetti
  orcid: 0000-0002-9645-6576
- first_name: Paul
  full_name: Robin, Paul
  id: 48c58128-57b0-11ee-9095-dc28fd97fc1d
  last_name: Robin
  orcid: 0000-0002-5728-9189
- first_name: Ivan
  full_name: Palaia, Ivan
  id: 9c805cd2-4b75-11ec-a374-db6dd0ed57fa
  last_name: Palaia
  orcid: ' 0000-0002-8843-9485 '
- first_name: Alberto
  full_name: Hernandez-Armendariz, Alberto
  last_name: Hernandez-Armendariz
- first_name: Sara
  full_name: Cuylen-Haering, Sara
  last_name: Cuylen-Haering
- first_name: Anđela
  full_name: Šarić, Anđela
  id: bf63d406-f056-11eb-b41d-f263a6566d8b
  last_name: Šarić
  orcid: 0000-0002-7854-2139
citation:
  ama: Sorichetti V, Robin P, Palaia I, Hernandez-Armendariz A, Cuylen-Haering S,
    Šarić A. Charge distribution of the coating brush drives interchromosome attraction.
    <i>PRX Life</i>. 2025;3(3). doi:<a href="https://doi.org/10.1103/41fd-r847">10.1103/41fd-r847</a>
  apa: Sorichetti, V., Robin, P., Palaia, I., Hernandez-Armendariz, A., Cuylen-Haering,
    S., &#38; Šarić, A. (2025). Charge distribution of the coating brush drives interchromosome
    attraction. <i>PRX Life</i>. American Physical Society. <a href="https://doi.org/10.1103/41fd-r847">https://doi.org/10.1103/41fd-r847</a>
  chicago: Sorichetti, Valerio, Paul Robin, Ivan Palaia, Alberto Hernandez-Armendariz,
    Sara Cuylen-Haering, and Anđela Šarić. “Charge Distribution of the Coating Brush
    Drives Interchromosome Attraction.” <i>PRX Life</i>. American Physical Society,
    2025. <a href="https://doi.org/10.1103/41fd-r847">https://doi.org/10.1103/41fd-r847</a>.
  ieee: V. Sorichetti, P. Robin, I. Palaia, A. Hernandez-Armendariz, S. Cuylen-Haering,
    and A. Šarić, “Charge distribution of the coating brush drives interchromosome
    attraction,” <i>PRX Life</i>, vol. 3, no. 3. American Physical Society, 2025.
  ista: Sorichetti V, Robin P, Palaia I, Hernandez-Armendariz A, Cuylen-Haering S,
    Šarić A. 2025. Charge distribution of the coating brush drives interchromosome
    attraction. PRX Life. 3(3), 033010.
  mla: Sorichetti, Valerio, et al. “Charge Distribution of the Coating Brush Drives
    Interchromosome Attraction.” <i>PRX Life</i>, vol. 3, no. 3, 033010, American
    Physical Society, 2025, doi:<a href="https://doi.org/10.1103/41fd-r847">10.1103/41fd-r847</a>.
  short: V. Sorichetti, P. Robin, I. Palaia, A. Hernandez-Armendariz, S. Cuylen-Haering,
    A. Šarić, PRX Life 3 (2025).
corr_author: '1'
date_created: 2026-02-16T14:50:32Z
date_published: 2025-08-11T00:00:00Z
date_updated: 2026-02-17T11:16:26Z
day: '11'
ddc:
- '570'
department:
- _id: AnSa
- _id: EdHa
doi: 10.1103/41fd-r847
ec_funded: 1
file:
- access_level: open_access
  checksum: 1702b9bdbfd902a7c08aa4f1479b390d
  content_type: application/pdf
  creator: dernst
  date_created: 2026-02-17T11:12:30Z
  date_updated: 2026-02-17T11:12:30Z
  file_id: '21287'
  file_name: 2025_PRXLife_Sorichetti.pdf
  file_size: 3732843
  relation: main_file
  success: 1
file_date_updated: 2026-02-17T11:12:30Z
has_accepted_license: '1'
intvolume: '         3'
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
  call_identifier: H2020
  grant_number: '802960'
  name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
  call_identifier: H2020
  grant_number: '101034413'
  name: 'IST-BRIDGE: International postdoctoral program'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
  name: EMBO Young Investigator Program - Andela Saric
publication: PRX Life
publication_identifier:
  eissn:
  - 2835-8279
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
status: public
title: Charge distribution of the coating brush drives interchromosome attraction
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21256'
abstract:
- lang: eng
  text: Collagen IV is one of the main components of the basement membrane, a layer
    of material that lines the majority of tissues in multicellular organisms. Collagen
    IV molecules assemble into networks, providing stiffness and elasticity to tissues
    and informing cell and organ shape, especially during development. In this work,
    we develop two coarse-grained models for collagen IV molecules that retain biochemical
    bond specificity and coarse grain at different length scales. Through molecular-dynamics
    simulations, we test the assembly and mechanics of the resulting networks and
    measure their response to strain in terms of stress, microscopic alignment, and
    bond dynamics. Within the basement membrane, collagen IV networks rearrange by
    molecule turnover, which affects tissue organization and can be linked with enzyme
    activity. Here we explore network rearrangements via bond remodeling, the process
    of breaking and remaking of bonds between network molecules. We then investigate
    the effects of active (enzymatic) bond remodeling. We find that this nonequilibrium
    remodeling allows a network to keep its integrity under strain, while relaxing
    fully over a variety of timescales, a dynamic response that is unavailable to
    networks undergoing equilibrium remodeling.
acknowledgement: This work received funding from the European Research Council under
  the European Union's Horizon 2020 research and innovation program through Grant
  Agreement No. 802960 (B.M., V.S., I.P., and A.Š.), the European Union's Horizon
  2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement
  No. 101034413 (I.P.), the NOMIS Foundation (F.P.-V.), the National Centre for the
  Replacement, Refinement and Reduction of Animals in Research Grant No. NC/T002425/1
  (N.K.), Leverhulme Trust project Grant No. RPG-2020-068 (N.K.), MRC Fellowship No.
  MR/W027437/1 (Y.M.), a Lister Institute Research Prize (Y.M.) and EMBO Young Investigator
  Programme (Y.M. and A.Š.).
article_number: '033019'
article_processing_charge: Yes
article_type: original
author:
- first_name: Billie
  full_name: Meadowcroft, Billie
  id: a4725fd6-932b-11ed-81e2-c098c7f37ae1
  last_name: Meadowcroft
  orcid: 0000-0003-3441-1337
- first_name: Valerio
  full_name: Sorichetti, Valerio
  id: ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b
  last_name: Sorichetti
  orcid: 0000-0002-9645-6576
- first_name: Eryk
  full_name: Ratajczyk, Eryk
  last_name: Ratajczyk
- first_name: Fernanda L
  full_name: Perez Verdugo, Fernanda L
  id: 4ecec223-9070-11ef-a0a9-bc76077bea8d
  last_name: Perez Verdugo
- first_name: Nargess
  full_name: Khalilgharibi, Nargess
  last_name: Khalilgharibi
- first_name: Yanlan
  full_name: Mao, Yanlan
  last_name: Mao
- first_name: Ivan
  full_name: Palaia, Ivan
  id: 9c805cd2-4b75-11ec-a374-db6dd0ed57fa
  last_name: Palaia
  orcid: ' 0000-0002-8843-9485 '
- first_name: Anđela
  full_name: Šarić, Anđela
  id: bf63d406-f056-11eb-b41d-f263a6566d8b
  last_name: Šarić
  orcid: 0000-0002-7854-2139
citation:
  ama: Meadowcroft B, Sorichetti V, Ratajczyk E, et al. Nonequilibrium remodeling
    of collagen IV networks in Silico. <i>PRX Life</i>. 2025;3. doi:<a href="https://doi.org/10.1103/gdd5-rnh7">10.1103/gdd5-rnh7</a>
  apa: Meadowcroft, B., Sorichetti, V., Ratajczyk, E., Perez Verdugo, F. L., Khalilgharibi,
    N., Mao, Y., … Šarić, A. (2025). Nonequilibrium remodeling of collagen IV networks
    in Silico. <i>PRX Life</i>. American Physical Society. <a href="https://doi.org/10.1103/gdd5-rnh7">https://doi.org/10.1103/gdd5-rnh7</a>
  chicago: Meadowcroft, Billie, Valerio Sorichetti, Eryk Ratajczyk, Fernanda L Perez
    Verdugo, Nargess Khalilgharibi, Yanlan Mao, Ivan Palaia, and Anđela Šarić. “Nonequilibrium
    Remodeling of Collagen IV Networks in Silico.” <i>PRX Life</i>. American Physical
    Society, 2025. <a href="https://doi.org/10.1103/gdd5-rnh7">https://doi.org/10.1103/gdd5-rnh7</a>.
  ieee: B. Meadowcroft <i>et al.</i>, “Nonequilibrium remodeling of collagen IV networks
    in Silico,” <i>PRX Life</i>, vol. 3. American Physical Society, 2025.
  ista: Meadowcroft B, Sorichetti V, Ratajczyk E, Perez Verdugo FL, Khalilgharibi
    N, Mao Y, Palaia I, Šarić A. 2025. Nonequilibrium remodeling of collagen IV networks
    in Silico. PRX Life. 3, 033019.
  mla: Meadowcroft, Billie, et al. “Nonequilibrium Remodeling of Collagen IV Networks
    in Silico.” <i>PRX Life</i>, vol. 3, 033019, American Physical Society, 2025,
    doi:<a href="https://doi.org/10.1103/gdd5-rnh7">10.1103/gdd5-rnh7</a>.
  short: B. Meadowcroft, V. Sorichetti, E. Ratajczyk, F.L. Perez Verdugo, N. Khalilgharibi,
    Y. Mao, I. Palaia, A. Šarić, PRX Life 3 (2025).
corr_author: '1'
date_created: 2026-02-16T15:55:03Z
date_published: 2025-09-05T00:00:00Z
date_updated: 2026-02-17T13:37:38Z
day: '05'
ddc:
- '570'
department:
- _id: AnSa
doi: 10.1103/gdd5-rnh7
ec_funded: 1
file:
- access_level: open_access
  checksum: 04cae5231d97e533145c493880fadbd9
  content_type: application/pdf
  creator: dernst
  date_created: 2026-02-17T13:36:01Z
  date_updated: 2026-02-17T13:36:01Z
  file_id: '21308'
  file_name: 2025_PRXLife_Meadowcroft.pdf
  file_size: 2277704
  relation: main_file
  success: 1
file_date_updated: 2026-02-17T13:36:01Z
has_accepted_license: '1'
intvolume: '         3'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
  call_identifier: H2020
  grant_number: '802960'
  name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
  call_identifier: H2020
  grant_number: '101034413'
  name: 'IST-BRIDGE: International postdoctoral program'
- _id: 349b6ff1-11ca-11ed-8bc3-f006047c2eeb
  name: EMBO Young Investigator Program - Andela Saric
publication: PRX Life
publication_identifier:
  eissn:
  - 2835-8279
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
status: public
title: Nonequilibrium remodeling of collagen IV networks in Silico
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2025'
...