[{"abstract":[{"text":"Collagens are fundamental components of extracellular matrices, requiring precise intracellular post-translational modifications for proper function. Among the modifications, prolyl 4-hydroxylation is critical to stabilise the collagen triple helix. In humans, this reaction is mediated by collagen prolyl 4-hydroxylases (P4Hs). While humans possess three genes encoding these enzymes (P4H⍺s), Drosophila melanogaster harbour at least 26 candidates for collagen P4H⍺s despite its simple genome, and it is poorly understood which of them are actually working on collagen in the fly. In this study, we addressed this question by carrying out thorough bioinformatic and biochemical analyses. We demonstrate that among the 26 potential collagen P4H⍺s, PH4⍺EFB shares the highest homology with vertebrate collagen P4H⍺s. Furthermore, while collagen P4Hs and their substrates must exist in the same cells, our transcriptomic analyses at the tissue and single cell levels showed a global co-expression of PH4⍺EFB but not the other P4H⍺-related genes with the collagen IV genes. Moreover, expression of PH4⍺EFB during embryogenesis was found to precede that of collagen IV, presumably enabling efficient collagen modification by PH4⍺EFB. Finally, biochemical assays confirm that PH4⍺EFB binds collagen, supporting its direct role in collagen IV modification. Collectively, we identify PH4⍺EFB as the primary and potentially constitutive prolyl 4-hydroxylase responsible for collagen IV biosynthesis in Drosophila. Our findings highlight the remarkably simple nature of Drosophila collagen IV biosynthesis, which may serve as a blueprint for defining the minimal requirements for collagen engineering.","lang":"eng"}],"date_created":"2025-09-28T22:01:26Z","file":[{"date_updated":"2026-01-05T13:09:01Z","creator":"dernst","access_level":"open_access","relation":"main_file","file_id":"20948","file_size":5844254,"checksum":"764257db41865d19daec1935788f72d7","success":1,"content_type":"application/pdf","date_created":"2026-01-05T13:09:01Z","file_name":"2025_MatrixBiology_Ishikawa.pdf"}],"oa":1,"type":"journal_article","quality_controlled":"1","status":"public","year":"2025","doi":"10.1016/j.matbio.2025.09.002","author":[{"full_name":"Ishikawa, Yoshihiro","last_name":"Ishikawa","first_name":"Yoshihiro"},{"last_name":"Toups","first_name":"Melissa A","orcid":"0000-0002-9752-7380","full_name":"Toups, Melissa A","id":"4E099E4E-F248-11E8-B48F-1D18A9856A87"},{"id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","full_name":"Elkrewi, Marwan N","first_name":"Marwan N","last_name":"Elkrewi","orcid":"0000-0002-5328-7231"},{"last_name":"Zajac","first_name":"Allison L.","full_name":"Zajac, Allison L."},{"first_name":"Sally","last_name":"Horne-Badovinac","full_name":"Horne-Badovinac, Sally"},{"full_name":"Matsubayashi, Yutaka","first_name":"Yutaka","last_name":"Matsubayashi"}],"project":[{"grant_number":"F8810","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","name":"The highjacking of meiosis for asexual reproduction"}],"page":"101-113","scopus_import":"1","day":"01","article_type":"original","PlanS_conform":"1","ddc":["570"],"month":"11","title":"Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila collagen IV","license":"https://creativecommons.org/licenses/by/4.0/","publication":"Matrix Biology","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"       141","file_date_updated":"2026-01-05T13:09:01Z","isi":1,"acknowledgement":"This project was supported by the All May See Foundation 7031,182 to YI, the Louisiana Board of Regents Support Fund: Research Competitiveness Subprogram to MAT, Austrian science fund (FWF) as part of the SFB Meiosis consortium FWF SFB F88-10 to Beatriz Vicoso (supported ME), American Heart Association 16POST2726018 and American Cancer Society 132,123-PF-18–025–01-CSM postdoctoral fellowships to ALZ, National Institutes of Health R01 GM136961 and R35 GM148485 to SH-B, and the Academy of Medical Sciences/the Wellcome Trust/ the Government Department of Business, Energy and Industrial Strategy/the British Heart Foundation/Diabetes UK Springboard Award SBF008\\1115 to YM. \r\nComputational analyses of single-nucleus transcriptome data were performed on the high performance computer (HPC) at Bournemouth University, the HPC at Institute of Science and Technology Austria, and the high-performance computational resources provided by the Louisiana Optical Network Infrastructure (http://www.loni.org). The authors are grateful to the researchers who published the transcriptome datasets [48,49,52,55] that became the essential bases for this study, to FlyBase for curating the datasets in an easily accessible format, and the Drosophila Genomics Resource Center (DGRC), supported by NIH grant 2P40OD010949, for providing the D17 cell line used in this research. The authors thank Kristian Koski (University of Oulu, Finland) for crucial advice on the domain structure of collagen P4H⍺s, and Ryusuke Niwa and Ryo Hoshino (University of Tsukuba, Japan) for helpful discussions on SP.","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"publisher":"Springer Nature","publication_status":"published","oa_version":"Published Version","date_published":"2025-11-01T00:00:00Z","volume":141,"external_id":{"isi":["001583892100002"],"pmid":["40946811"]},"department":[{"_id":"BeVi"}],"pmid":1,"citation":{"chicago":"Ishikawa, Yoshihiro, Melissa A Toups, Marwan N Elkrewi, Allison L. Zajac, Sally Horne-Badovinac, and Yutaka Matsubayashi. “Evidence for the Major Role of PH4⍺EFB in the Prolyl 4-Hydroxylation of Drosophila Collagen IV.” <i>Matrix Biology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1016/j.matbio.2025.09.002\">https://doi.org/10.1016/j.matbio.2025.09.002</a>.","ieee":"Y. Ishikawa, M. A. Toups, M. N. Elkrewi, A. L. Zajac, S. Horne-Badovinac, and Y. Matsubayashi, “Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila collagen IV,” <i>Matrix Biology</i>, vol. 141, no. 11. Springer Nature, pp. 101–113, 2025.","apa":"Ishikawa, Y., Toups, M. A., Elkrewi, M. N., Zajac, A. L., Horne-Badovinac, S., &#38; Matsubayashi, Y. (2025). Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila collagen IV. <i>Matrix Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1016/j.matbio.2025.09.002\">https://doi.org/10.1016/j.matbio.2025.09.002</a>","ama":"Ishikawa Y, Toups MA, Elkrewi MN, Zajac AL, Horne-Badovinac S, Matsubayashi Y. Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila collagen IV. <i>Matrix Biology</i>. 2025;141(11):101-113. doi:<a href=\"https://doi.org/10.1016/j.matbio.2025.09.002\">10.1016/j.matbio.2025.09.002</a>","mla":"Ishikawa, Yoshihiro, et al. “Evidence for the Major Role of PH4⍺EFB in the Prolyl 4-Hydroxylation of Drosophila Collagen IV.” <i>Matrix Biology</i>, vol. 141, no. 11, Springer Nature, 2025, pp. 101–13, doi:<a href=\"https://doi.org/10.1016/j.matbio.2025.09.002\">10.1016/j.matbio.2025.09.002</a>.","ista":"Ishikawa Y, Toups MA, Elkrewi MN, Zajac AL, Horne-Badovinac S, Matsubayashi Y. 2025. Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila collagen IV. Matrix Biology. 141(11), 101–113.","short":"Y. Ishikawa, M.A. Toups, M.N. Elkrewi, A.L. Zajac, S. Horne-Badovinac, Y. Matsubayashi, Matrix Biology 141 (2025) 101–113."},"publication_identifier":{"eissn":["1569-1802"],"issn":["0945-053X"]},"has_accepted_license":"1","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","issue":"11","article_processing_charge":"Yes (in subscription journal)","date_updated":"2026-01-05T13:09:08Z","_id":"20404"},{"_id":"20796","date_updated":"2025-12-15T09:28:37Z","article_processing_charge":"Yes","publication_identifier":{"eissn":["2041-1723"]},"citation":{"apa":"Paouneskou, D., Baudrimont, A., Kelemen, R. K., Elkrewi, M. N., Graf, A., Moukbel Ali Aldawla, S., … Jantsch, V. (2025). BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery is important for genome integrity. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-65420-9\">https://doi.org/10.1038/s41467-025-65420-9</a>","mla":"Paouneskou, Dimitra, et al. “BAF-1–VRK-1 Mediated Release of Meiotic Chromosomes from the Nuclear Periphery Is Important for Genome Integrity.” <i>Nature Communications</i>, vol. 16, 10446, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-65420-9\">10.1038/s41467-025-65420-9</a>.","ama":"Paouneskou D, Baudrimont A, Kelemen RK, et al. BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery is important for genome integrity. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-65420-9\">10.1038/s41467-025-65420-9</a>","ista":"Paouneskou D, Baudrimont A, Kelemen RK, Elkrewi MN, Graf A, Moukbel Ali Aldawla S, Kölbl C, Tiemann-Boege I, Vicoso B, Jantsch V. 2025. BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery is important for genome integrity. Nature Communications. 16, 10446.","short":"D. Paouneskou, A. Baudrimont, R.K. Kelemen, M.N. Elkrewi, A. Graf, S. Moukbel Ali Aldawla, C. Kölbl, I. Tiemann-Boege, B. Vicoso, V. Jantsch, Nature Communications 16 (2025).","chicago":"Paouneskou, Dimitra, Antoine Baudrimont, Réka K Kelemen, Marwan N Elkrewi, Angela Graf, Shehab Moukbel Ali Aldawla, Claudia Kölbl, Irene Tiemann-Boege, Beatriz Vicoso, and Verena Jantsch. “BAF-1–VRK-1 Mediated Release of Meiotic Chromosomes from the Nuclear Periphery Is Important for Genome Integrity.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-65420-9\">https://doi.org/10.1038/s41467-025-65420-9</a>.","ieee":"D. Paouneskou <i>et al.</i>, “BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery is important for genome integrity,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025."},"pmid":1,"department":[{"_id":"BeVi"}],"external_id":{"pmid":["41290579"]},"OA_type":"gold","OA_place":"publisher","language":[{"iso":"eng"}],"has_accepted_license":"1","publication_status":"published","publisher":"Springer Nature","date_published":"2025-11-25T00:00:00Z","volume":16,"oa_version":"Published Version","file_date_updated":"2025-12-15T09:25:51Z","intvolume":"        16","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Nature Communications","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"acknowledgement":"We are grateful to Monique Zetka, Nicola Silva, and Yumi Kim, Needhi Bhalla, George Krohne and Rueyling Lin for providing reagents; Scott Kennedy for sharing the multiplexed FISH library; and members of the Max Perutz Labs’ BioOptics facility (Irmgard Fischer, Josef Gotzmann, Thomas Peterbauer, Clara Bodner, and Nick Wedige) for training and support in image acquisition. We also thank the members of the NGS facility at the Vienna Biocenter. This work was funded by the Austrian Science Fund (FWF) SFB projects F 8805-B (VJ), https://doi.org/10.55776/F88, F 8809-B (ITB), and F8810-B (BV). We are also grateful to members of the V. Jantsch laboratory for helpful discussions. Some strains were provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health Office of Research Infrastructure Programs (P40OD010440).","ddc":["570"],"PlanS_conform":"1","title":"BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery is important for genome integrity","month":"11","day":"25","DOAJ_listed":"1","scopus_import":"1","author":[{"full_name":"Paouneskou, Dimitra","last_name":"Paouneskou","first_name":"Dimitra"},{"first_name":"Antoine","last_name":"Baudrimont","full_name":"Baudrimont, Antoine"},{"full_name":"Kelemen, Réka K","id":"48D3F8DE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8489-9281","last_name":"Kelemen","first_name":"Réka K"},{"full_name":"Elkrewi, Marwan N","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","orcid":"0000-0002-5328-7231","first_name":"Marwan N","last_name":"Elkrewi"},{"full_name":"Graf, Angela","first_name":"Angela","last_name":"Graf"},{"first_name":"Shehab","last_name":"Moukbel Ali Aldawla","full_name":"Moukbel Ali Aldawla, Shehab"},{"first_name":"Claudia","last_name":"Kölbl","full_name":"Kölbl, Claudia"},{"full_name":"Tiemann-Boege, Irene","last_name":"Tiemann-Boege","first_name":"Irene"},{"full_name":"Vicoso, Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz"},{"full_name":"Jantsch, Verena","last_name":"Jantsch","first_name":"Verena"}],"project":[{"name":"The highjacking of meiosis for asexual reproduction","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","grant_number":"F8810"}],"year":"2025","doi":"10.1038/s41467-025-65420-9","article_type":"original","article_number":"10446","status":"public","quality_controlled":"1","date_created":"2025-12-11T10:45:06Z","abstract":[{"text":"Rapid prophase chromosome movements ensure faithful alignment of the parental homologous chromosomes and successful synapsis formation during meiosis. These movements are driven by cytoplasmic forces transmitted to the nuclear periphery, where chromosome ends are attached through transmembrane proteins. During many developmental stages a specific genome architecture with chromatin nuclear periphery contacts mediates specific gene expression. Whether chromatin is removed from the nuclear periphery as a consequence of chromosome motions or by a specific mechanism is not fully understood. Here, we identify a mechanism to remove chromatin from the nuclear periphery through vaccinia related kinase (VRK-1)–dependent phosphorylation of Barrier to Autointegration Factor 1 (BAF-1) in Caenorhabditis elegans early prophase of meiosis. Interfering with chromatin removal delays chromosome pairing, impairs synapsis, produces oocytes with abnormal chromosomes and elevated apoptosis. Long read sequencing reveals deletions and duplications in offspring lacking VRK-1 underscoring the importance of the BAF-1–VRK-1 module in preserving genome stability in gametes during rapid chromosome movements.","lang":"eng"}],"type":"journal_article","file":[{"success":1,"file_name":"2025_NatureComm_Paouneskou.pdf","date_created":"2025-12-15T09:25:51Z","content_type":"application/pdf","file_id":"20823","checksum":"a952f7ea050242b79008540de49a0e61","file_size":8096309,"creator":"dernst","access_level":"open_access","date_updated":"2025-12-15T09:25:51Z","relation":"main_file"}],"oa":1},{"article_type":"original","article_number":"msaf085","DOAJ_listed":"1","day":"01","scopus_import":"1","author":[{"first_name":"Vincent K","last_name":"Bett","full_name":"Bett, Vincent K","id":"57854184-AAE0-11E9-8D04-98D6E5697425"},{"id":"2b681148-eed5-11eb-b81b-ae229e8620f8","full_name":"Trejo Arellano, Minerva S","first_name":"Minerva S","last_name":"Trejo Arellano","orcid":"0000-0002-1982-3475"},{"full_name":"Vicoso, Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz"}],"project":[{"name":"Sex chromosomes in evolution and development","grant_number":"PAT 8748323","_id":"8ed82125-16d5-11f0-9cad-fbcae312235b"},{"_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","grant_number":"F8810","name":"The highjacking of meiosis for asexual reproduction"}],"doi":"10.1093/molbev/msaf085","year":"2025","title":"Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana","month":"05","related_material":{"record":[{"relation":"dissertation_contains","status":"private","id":"20444"},{"relation":"dissertation_contains","status":"public","id":"20449"}],"link":[{"relation":"software","url":"https://github.com/vkb25/Chromatin-landscape-in-Artemia-franciscana.git"}]},"ddc":["570"],"type":"journal_article","file":[{"file_name":"2025_MBE_Bett.pdf","content_type":"application/pdf","date_created":"2025-05-28T09:34:36Z","success":1,"checksum":"6c14b03f94b4aadf8869be2c4366d077","file_size":1282772,"file_id":"19756","relation":"main_file","creator":"dernst","access_level":"open_access","date_updated":"2025-05-28T09:34:36Z"}],"oa":1,"acknowledged_ssus":[{"_id":"ScienComp"}],"date_created":"2025-05-25T22:16:56Z","abstract":[{"lang":"eng","text":"The males and females of the brine shrimp Artemia franciscana are highly dimorphic, and this dimorphism is associated with substantial sex-biased gene expression in heads and gonads. How these sex-specific patterns of expression are regulated at the molecular level is unknown. A. franciscana also has differentiated ZW sex chromosomes, with complete dosage compensation, but the molecular mechanism through which compensation is achieved is unknown. Here, we conducted CUT&TAG assays targeting 7 post-translational histone modifications (H3K27me3, H3K9me2, H3K9me3, H3K36me3, H3K27ac, H3K4me3, and H4K16ac) in heads and gonads of A. franciscana, allowing us to divide the genome into 12 chromatin states. We further defined functional chromatin signatures for all genes, which were correlated with transcript level abundances. Differences in the occupancy of the profiled epigenetic marks between sexes were associated with differential gene expression between males and females. Finally, we found a significant enrichment of the permissive H4K16ac histone mark in the Z-specific region in both tissues of females but not males, supporting the role of this histone mark in mediating dosage compensation of the Z chromosome."}],"status":"public","quality_controlled":"1","corr_author":"1","OA_place":"publisher","language":[{"iso":"eng"}],"OA_type":"gold","has_accepted_license":"1","publication_identifier":{"eissn":["1537-1719"],"issn":["0737-4038"]},"citation":{"ieee":"V. K. Bett, M. S. Trejo Arellano, and B. Vicoso, “Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana,” <i>Molecular Biology and Evolution</i>, vol. 42, no. 5. Oxford University Press, 2025.","chicago":"Bett, Vincent K, Minerva S Trejo Arellano, and Beatriz Vicoso. “Chromatin Landscape Is Associated with Sex-Biased Expression and Drosophila-like Dosage Compensation of the Z Chromosome in Artemia Franciscana.” <i>Molecular Biology and Evolution</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/molbev/msaf085\">https://doi.org/10.1093/molbev/msaf085</a>.","short":"V.K. Bett, M.S. Trejo Arellano, B. Vicoso, Molecular Biology and Evolution 42 (2025).","ista":"Bett VK, Trejo Arellano MS, Vicoso B. 2025. Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana. Molecular Biology and Evolution. 42(5), msaf085.","ama":"Bett VK, Trejo Arellano MS, Vicoso B. Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana. <i>Molecular Biology and Evolution</i>. 2025;42(5). doi:<a href=\"https://doi.org/10.1093/molbev/msaf085\">10.1093/molbev/msaf085</a>","mla":"Bett, Vincent K., et al. “Chromatin Landscape Is Associated with Sex-Biased Expression and Drosophila-like Dosage Compensation of the Z Chromosome in Artemia Franciscana.” <i>Molecular Biology and Evolution</i>, vol. 42, no. 5, msaf085, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/molbev/msaf085\">10.1093/molbev/msaf085</a>.","apa":"Bett, V. K., Trejo Arellano, M. S., &#38; Vicoso, B. (2025). Chromatin landscape is associated with sex-biased expression and Drosophila-like dosage compensation of the Z chromosome in Artemia franciscana. <i>Molecular Biology and Evolution</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/molbev/msaf085\">https://doi.org/10.1093/molbev/msaf085</a>"},"pmid":1,"department":[{"_id":"BeVi"},{"_id":"DaZi"}],"external_id":{"pmid":["40202086"],"isi":["001483460200001"]},"_id":"19735","date_updated":"2026-04-07T12:28:15Z","article_processing_charge":"Yes","issue":"5","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"acknowledgement":"We thank the Vicoso lab for their help in maintaining Artemia and for their valuable feedback and suggestions. We thank Marwan Elkrewi for his useful technical advice and discussions. We are also grateful to the Scientific Unit at ISTA Austria for computational resources and assistance. This work was supported by Austrian science fund (FWF) grants PAT8748323 and SFB F88-10 (as part of the SFB Meiosis consortium https://sfbmeiosis.org) to BV and Swedish Research Council (Vetenskapsrådet, grant number 2020-06424) to MSTA.","isi":1,"intvolume":"        42","file_date_updated":"2025-05-28T09:34:36Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication":"Molecular Biology and Evolution","volume":42,"date_published":"2025-05-01T00:00:00Z","oa_version":"Published Version","publication_status":"published","publisher":"Oxford University Press"},{"ddc":["576"],"related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"19735"},{"id":"15009","relation":"part_of_dissertation","status":"public"}]},"degree_awarded":"PhD","month":"10","title":"Evolution and regulation of the Z chromosome","project":[{"name":"Sex chromosomes in evolution and development","grant_number":"PAT 8748323","_id":"8ed82125-16d5-11f0-9cad-fbcae312235b"},{"name":"The highjacking of meiosis for asexual reproduction","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","grant_number":"F8810"}],"author":[{"full_name":"Bett, Vincent K","id":"57854184-AAE0-11E9-8D04-98D6E5697425","first_name":"Vincent K","last_name":"Bett"}],"doi":"10.15479/AT-ISTA-20449","year":"2025","day":"10","page":"114","corr_author":"1","status":"public","date_created":"2025-10-11T08:18:51Z","file":[{"date_updated":"2025-10-20T13:32:29Z","access_level":"closed","creator":"vbett","relation":"main_file","file_id":"20507","file_size":18507283,"checksum":"26905c22bca417198a733d792d8ce422","embargo":"2026-06-01","content_type":"application/pdf","embargo_to":"open_access","date_created":"2025-10-20T13:32:29Z","file_name":"2025_Bett_Vincent_Thesis.pdf"},{"file_size":17163921,"checksum":"6a09a8d126d3628bfd8a35202735f267","file_id":"20508","relation":"source_file","date_updated":"2025-11-06T12:42:09Z","access_level":"closed","creator":"vbett","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2025-10-20T13:35:34Z","file_name":"2025_Bett_Vincent_Thesis.docx"}],"acknowledged_ssus":[{"_id":"ScienComp"}],"type":"dissertation","date_updated":"2026-04-07T12:28:15Z","article_processing_charge":"No","_id":"20449","citation":{"chicago":"Bett, Vincent K. “Evolution and Regulation of the Z Chromosome.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20449\">https://doi.org/10.15479/AT-ISTA-20449</a>.","ieee":"V. K. Bett, “Evolution and regulation of the Z chromosome,” Institute of Science and Technology Austria, 2025.","mla":"Bett, Vincent K. <i>Evolution and Regulation of the Z Chromosome</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20449\">10.15479/AT-ISTA-20449</a>.","ama":"Bett VK. Evolution and regulation of the Z chromosome. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20449\">10.15479/AT-ISTA-20449</a>","ista":"Bett VK. 2025. Evolution and regulation of the Z chromosome. Institute of Science and Technology Austria.","short":"V.K. Bett, Evolution and Regulation of the Z Chromosome, Institute of Science and Technology Austria, 2025.","apa":"Bett, V. K. (2025). <i>Evolution and regulation of the Z chromosome</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20449\">https://doi.org/10.15479/AT-ISTA-20449</a>"},"publication_identifier":{"issn":["2663-337X"]},"department":[{"_id":"GradSch"},{"_id":"BeVi"}],"language":[{"iso":"eng"}],"OA_place":"publisher","has_accepted_license":"1","publication_status":"published","publisher":"Institute of Science and Technology Austria","oa_version":"Published Version","date_published":"2025-10-10T00:00:00Z","file_date_updated":"2025-11-06T12:42:09Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","alternative_title":["ISTA Thesis"],"supervisor":[{"last_name":"Vicoso","first_name":"Beatriz","orcid":"0000-0002-4579-8306","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","full_name":"Vicoso, Beatriz"}],"acknowledgement":"This work was supported by the Austrian Science Fund (FWF) through grants PAT8748323\r\nand SFB F88-10 awarded to Professor Beatriz Vicoso.","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"}},{"has_accepted_license":"1","language":[{"iso":"eng"}],"OA_place":"publisher","department":[{"_id":"GradSch"},{"_id":"BeVi"}],"publication_identifier":{"isbn":["9783990780534"],"eissn":["2663-337X"]},"citation":{"short":"M.N. Elkrewi, Evolution of Sex Chromosomes, Sex Determination and Asexuality in Artemia Brine Shrimp, Institute of Science and Technology Austria, 2025.","ista":"Elkrewi MN. 2025. Evolution of sex chromosomes, sex determination and asexuality in Artemia brine shrimp. Institute of Science and Technology Austria.","ama":"Elkrewi MN. Evolution of sex chromosomes, sex determination and asexuality in Artemia brine shrimp. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19386\">10.15479/AT-ISTA-19386</a>","mla":"Elkrewi, Marwan N. <i>Evolution of Sex Chromosomes, Sex Determination and Asexuality in Artemia Brine Shrimp</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19386\">10.15479/AT-ISTA-19386</a>.","apa":"Elkrewi, M. N. (2025). <i>Evolution of sex chromosomes, sex determination and asexuality in Artemia brine shrimp</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19386\">https://doi.org/10.15479/AT-ISTA-19386</a>","ieee":"M. N. Elkrewi, “Evolution of sex chromosomes, sex determination and asexuality in Artemia brine shrimp,” Institute of Science and Technology Austria, 2025.","chicago":"Elkrewi, Marwan N. “Evolution of Sex Chromosomes, Sex Determination and Asexuality in Artemia Brine Shrimp.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19386\">https://doi.org/10.15479/AT-ISTA-19386</a>."},"article_processing_charge":"No","date_updated":"2026-04-16T12:20:41Z","_id":"19386","OA_embargo":"12","alternative_title":["ISTA Thesis"],"acknowledgement":"My PhD work was funded by the Austrian science fund (FWF), as part of the SFB Meiosis consortium (https://sfbmeiosis.org/, grant ID FWF SFB F88-10).","supervisor":[{"last_name":"Vicoso","first_name":"Beatriz","orcid":"0000-0002-4579-8306","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","full_name":"Vicoso, Beatriz"}],"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","file_date_updated":"2026-03-26T23:30:03Z","oa_version":"Published Version","date_published":"2025-03-14T00:00:00Z","publisher":"Institute of Science and Technology Austria","publication_status":"published","doi":"10.15479/AT-ISTA-19386","year":"2025","project":[{"name":"The highjacking of meiosis for asexual reproduction","grant_number":"F8810","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396"}],"author":[{"full_name":"Elkrewi, Marwan N","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","orcid":"0000-0002-5328-7231","first_name":"Marwan N","last_name":"Elkrewi"}],"page":"170","day":"14","month":"03","title":"Evolution of sex chromosomes, sex determination and asexuality in Artemia brine shrimp","ddc":["570","576"],"degree_awarded":"PhD","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"12248"},{"relation":"part_of_dissertation","status":"public","id":"10167"},{"relation":"part_of_dissertation","status":"public","id":"10767"},{"relation":"part_of_dissertation","status":"public","id":"15009"},{"status":"public","relation":"part_of_dissertation","id":"14613"},{"relation":"part_of_dissertation","status":"public","id":"17890"}]},"oa":1,"acknowledged_ssus":[{"_id":"ScienComp"}],"file":[{"checksum":"5549a8216c07e4c39281648912d72246","file_size":25019680,"file_id":"19462","relation":"source_file","creator":"melkrewi","access_level":"closed","date_updated":"2026-03-26T23:30:03Z","file_name":"Thesis_Marwan_Elkrewi.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2025-03-26T07:06:56Z","embargo_to":"open_access"},{"file_id":"19463","checksum":"aed2ba9965aa89b3414deae1ae9f4321","file_size":17294844,"access_level":"open_access","creator":"melkrewi","date_updated":"2026-03-26T23:30:03Z","relation":"main_file","embargo":"2026-03-26","file_name":"Thesis_Marwan_Elkrewi.pdf","date_created":"2025-03-26T07:06:22Z","content_type":"application/pdf"}],"type":"dissertation","abstract":[{"text":"Crustaceans are a large group of arthropods with a great diversity of species and\r\ndifferent types of sex determination systems and reproductive modes (Subramoniam, 2017).\r\nThis makes them a great model for exploring the evolution of sex chromosomes and sexual\r\ndimorphism and investigating the evolutionary mechanisms driving and maintaining the\r\ndiversity of reproductive systems. Within this taxon, Brine shrimp of the genus Artemia, a\r\nbranchiopod crustacean, are well suited for such explorations, as they have both highly\r\ndimorphic traits and closely related sexual and asexual species. Although brine shrimp are\r\nknown to have ZW sex chromosomes (Bowen, 1963; Parraguez et al., 2009), the sex\r\nchromosomes are still not well characterized at the genomic level, the sex-determination gene\r\nis unknown, and it is still unclear whether the same sex chromosomes as shared by the\r\ndifferent species.\r\nThe first part of this thesis was to characterize the Z and W chromosomes in Artemia\r\nusing an array of methods, from generating multiple chromosome and contig level genome\r\nassemblies to identifying W-linked scaffolds and transcripts in multiple species using k-mer\r\nbased approaches.\r\nThe second part tackles the conservation of the cell type specific regulatory pathways\r\nin the female reproductive system between Artemia and Drosophila, and the expression of the\r\nZ-specific region throughout meiosis using single-nucleus RNA-seq data. Our results show\r\nthat germline cells lack dosage compensation, with a subset of cells showing evidence of\r\nextreme repression of the Z chromosome.\r\nWith multiple sexual species and several asexual lineages of parthenogenetic females\r\nthat produce rare males at low frequencies, Brine shrimp present the perfect opportunity to\r\nexplore the transition to asexuality and shed light on the prerequisites and repercussions of\r\nthe form of modified meiosis maintaining the asexual lineages. The last chapter is an\r\ninvestigation of the molecular pathways involved in asexual reproduction in Artemia using\r\nnewly generated single nucleus RNAseq and WGS data and previously published data. ","lang":"eng"}],"date_created":"2025-03-11T12:54:31Z","status":"public","corr_author":"1"},{"file":[{"file_name":"readme.txt.txt","date_created":"2023-12-22T13:54:21Z","content_type":"text/plain","success":1,"relation":"main_file","access_level":"open_access","creator":"melkrewi","date_updated":"2023-12-22T13:54:21Z","checksum":"bdaf1392867786634ec5466d528c36ca","file_size":847,"file_id":"14707"},{"success":1,"file_name":"data_artemia_franciscana_genome.zip","content_type":"application/x-zip-compressed","date_created":"2023-12-22T14:14:06Z","file_id":"14708","checksum":"973e1cbdab923a71709782177980829f","file_size":343632753,"access_level":"open_access","creator":"melkrewi","date_updated":"2023-12-22T14:14:06Z","relation":"main_file"}],"oa":1,"keyword":["sex chromosome evolution","genome assembly","dosage compensation"],"type":"research_data","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"abstract":[{"lang":"eng","text":"Since the commercialization of brine shrimp (genus Artemia) in the 1950s, this lineage, and in particular the model species Artemia franciscana, has been the subject of extensive research. However, our understanding of the genetic mechanisms underlying various aspects of their reproductive biology, including sex determination, are still lacking. This is partly due to the scarcity of genomic resources for Artemia species and crustaceans in general. Here, we present a chromosome-level genome assembly of Artemia franciscana (Kellogg 1906), from the Great Salt Lake, USA. The genome is 1GB, and the majority of the genome (81%) is scaffolded into 21 linkage groups using a previously published high-density linkage map. We performed coverage and FST analyses using male and female genomic and transcriptomic reads to quantify the extent of differentiation between the Z and W chromosomes. Additionally, we quantified the expression levels in male and female heads and gonads and found further evidence for dosage compensation in this species."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2023-12-22T13:40:48Z","file_date_updated":"2023-12-22T14:14:06Z","oa_version":"Published Version","status":"public","date_published":"2024-01-02T00:00:00Z","publisher":"Institute of Science and Technology Austria","corr_author":"1","contributor":[{"last_name":"Bett","first_name":"Vincent K","contributor_type":"researcher","id":"57854184-AAE0-11E9-8D04-98D6E5697425"},{"id":"2A0848E2-F248-11E8-B48F-1D18A9856A87","contributor_type":"project_member","first_name":"Ariana","last_name":"Macon"},{"id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","contributor_type":"supervisor","orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz"},{"first_name":"Marwan N","last_name":"Elkrewi","orcid":"0000-0002-5328-7231","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","contributor_type":"researcher"}],"has_accepted_license":"1","year":"2024","doi":"10.15479/AT:ISTA:14705","author":[{"orcid":"0000-0002-5328-7231","last_name":"Elkrewi","first_name":"Marwan N","full_name":"Elkrewi, Marwan N","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425"}],"project":[{"name":"The highjacking of meiosis for asexual reproduction","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","grant_number":"F8810"}],"department":[{"_id":"GradSch"},{"_id":"BeVi"}],"day":"02","citation":{"apa":"Elkrewi, M. N. (2024). Data from “Chromosome-level assembly of Artemia franciscana sheds light on sex-chromosome differentiation.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:14705\">https://doi.org/10.15479/AT:ISTA:14705</a>","ista":"Elkrewi MN. 2024. Data from ‘Chromosome-level assembly of Artemia franciscana sheds light on sex-chromosome differentiation’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:14705\">10.15479/AT:ISTA:14705</a>.","short":"M.N. Elkrewi, (2024).","mla":"Elkrewi, Marwan N. <i>Data from “Chromosome-Level Assembly of Artemia Franciscana Sheds Light on Sex-Chromosome Differentiation.”</i> Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:14705\">10.15479/AT:ISTA:14705</a>.","ama":"Elkrewi MN. Data from “Chromosome-level assembly of Artemia franciscana sheds light on sex-chromosome differentiation.” 2024. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:14705\">10.15479/AT:ISTA:14705</a>","ieee":"M. N. Elkrewi, “Data from ‘Chromosome-level assembly of Artemia franciscana sheds light on sex-chromosome differentiation.’” Institute of Science and Technology Austria, 2024.","chicago":"Elkrewi, Marwan N. “Data from ‘Chromosome-Level Assembly of Artemia Franciscana Sheds Light on Sex-Chromosome Differentiation.’” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/AT:ISTA:14705\">https://doi.org/10.15479/AT:ISTA:14705</a>."},"month":"01","article_processing_charge":"No","date_updated":"2025-09-04T12:05:42Z","title":"Data from \"Chromosome-level assembly of Artemia franciscana sheds light on sex-chromosome differentiation\"","_id":"14705","ddc":["576"],"related_material":{"record":[{"id":"15009","status":"public","relation":"used_in_publication"}]}},{"type":"research_data","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"acknowledged_ssus":[{"_id":"ScienComp"}],"file":[{"file_size":2465,"checksum":"26b5d41b3103f4284dd97d56e370a5b6","file_id":"17394","relation":"main_file","date_updated":"2024-08-05T22:24:18Z","creator":"melkrewi","access_level":"open_access","content_type":"text/plain","date_created":"2024-08-05T22:24:18Z","file_name":"README.txt","success":1},{"file_size":2526735400,"checksum":"95adab5e36148015da313505e3910707","file_id":"17395","relation":"main_file","date_updated":"2024-08-05T23:28:52Z","access_level":"open_access","creator":"melkrewi","content_type":"application/x-zip-compressed","date_created":"2024-08-05T23:28:52Z","file_name":"Data_artemia_single_nucleus_atlas.zip","success":1}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2024-08-05T23:28:52Z","date_created":"2024-08-02T07:27:45Z","abstract":[{"text":"This is the supplementary data for the paper titled \"Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome\", where we described the generation and analysis of single-nucleus expression and chromatin-accessibility data from the female reproductive system of Artemia franciscana. We compared our dataset to the published Drosophila single-nucleus data (over 400 million years of divergence) and highlighted the extreme conservation of several of the molecular pathways of oogenesis and meiosis. We found evidence of global transcriptional quiescence and chromatin condensation in late germ cells, highlighting the conserved role of this repressive stage in arthropod oogenesis. Additionally, we explored the expression patterns of the ZW sex chromosomes during oogenesis. Our data shows that the Z-chromosome is consistently downregulated in germline cells. While this is partly driven by a lack of dosage compensation in the germline, a subset of cells show stronger repression of the Z chromosome.","lang":"eng"}],"status":"public","date_published":"2024-08-05T00:00:00Z","oa_version":"Published Version","corr_author":"1","publisher":"Institute of Science and Technology Austria","has_accepted_license":"1","department":[{"_id":"GradSch"},{"_id":"BeVi"}],"day":"05","citation":{"ieee":"M. N. Elkrewi and B. Vicoso, “Data for: ‘Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome.’” Institute of Science and Technology Austria, 2024.","chicago":"Elkrewi, Marwan N, and Beatriz Vicoso. “Data for: ‘Single-Nucleus Atlas of the Artemia Female Reproductive System Suggests Germline Repression of the Z Chromosome.’” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/AT:ISTA:17362\">https://doi.org/10.15479/AT:ISTA:17362</a>.","ista":"Elkrewi MN, Vicoso B. 2024. Data for: ‘Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:17362\">10.15479/AT:ISTA:17362</a>.","short":"M.N. Elkrewi, B. Vicoso, (2024).","mla":"Elkrewi, Marwan N., and Beatriz Vicoso. <i>Data for: “Single-Nucleus Atlas of the Artemia Female Reproductive System Suggests Germline Repression of the Z Chromosome.”</i> Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:17362\">10.15479/AT:ISTA:17362</a>.","ama":"Elkrewi MN, Vicoso B. Data for: “Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome.” 2024. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:17362\">10.15479/AT:ISTA:17362</a>","apa":"Elkrewi, M. N., &#38; Vicoso, B. (2024). Data for: “Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:17362\">https://doi.org/10.15479/AT:ISTA:17362</a>"},"doi":"10.15479/AT:ISTA:17362","year":"2024","author":[{"orcid":"0000-0002-5328-7231","last_name":"Elkrewi","first_name":"Marwan N","full_name":"Elkrewi, Marwan N","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425"},{"full_name":"Vicoso, Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz"}],"project":[{"name":"The highjacking of meiosis for asexual reproduction","grant_number":"F8810","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396"}],"title":"Data for: \"Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome\"","_id":"17362","article_processing_charge":"No","month":"08","date_updated":"2026-04-16T12:20:41Z","related_material":{"record":[{"id":"17890","status":"public","relation":"used_in_publication"}]},"ddc":["576"]},{"day":"20","page":"105","author":[{"orcid":"0000-0002-8489-9281","first_name":"Réka K","last_name":"Kelemen","id":"48D3F8DE-F248-11E8-B48F-1D18A9856A87","full_name":"Kelemen, Réka K"}],"project":[{"name":"Prevalence and Influence of Sexual Antagonism on Genome Evolution","call_identifier":"H2020","grant_number":"715257","_id":"250BDE62-B435-11E9-9278-68D0E5697425"},{"name":"The highjacking of meiosis for asexual reproduction","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","grant_number":"F8810"}],"doi":"10.15479/at:ista:17119","year":"2024","degree_awarded":"PhD","related_material":{"record":[{"id":"542","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"10767"}]},"ddc":["576"],"title":"Characterizing the sequence and expression evolution of the t-haplotype, a model meiotic driver","month":"06","date_created":"2024-06-07T16:14:13Z","abstract":[{"lang":"eng","text":"Genomes are shaped by natural selection at the level of the organism, as genomic variants that\r\nhave a beneficial effect on the viability or fecundity of their carriers are on average expected\r\nto be passed on to more offspring than less beneficial alleles. However, selection also favors\r\ngenomic variants that drive their own transmission to the next generation above the mendelian\r\nexpectation of 50 percent in heterozygotes, even if these self-promoting variants are less\r\nbeneficial to the organism than other variants at the same locus. Such variants, called meiotic\r\ndrivers, are found in diverse taxa, and often impose fitness costs on their host organisms. As\r\nmeiotic drivers often require multiple genes and sequences for transmission ratio distortion,\r\nthey are often found in regions of low recombination, such as inversions, which prevent their\r\nrecombination with the non-driving homologous regions. Reduced recombination rates are\r\nexpected to lead to the accumulation of deleterious mutations, which may affect hundreds\r\nof genes trapped in the inversions of meiotic drivers. Although the observed fitness costs of\r\nself-promoting haplotypes are thought to possibly reflect sequence degeneration, no study has\r\nsystematically investigated the level of degeneration on a meiotic driver. Further, the low\r\nrates of recombination between driving and non-driving haplotypes have limited the power of\r\ntraditional genetic studies in uncovering the gene content of meiotic drivers, and made the\r\nthe identification of the genes causing transmission ratio distortion difficult.\r\nAfter an introduction to meiotic drivers in Chapter 1, this thesis presents three studies that\r\nmake use of next generation sequencing data to characterize the sequence and expression\r\nevolution of genes on the t-haplotype, a large and ancient meiotic driver in house mice that is\r\ntransmitted to up to 100% of the offspring in males heterozygous for it. Chapter 2 presents\r\na comprehensive assessment of the t-haplotype’s sequence evolution, which shows signs of\r\nsequence degeneration counteracted by occasional recombination with the non-driving homolog\r\nover large parts of the meiotic driver, proposing an explanation for its long-term survival.\r\nChapter 3 investigates the sequence and expression evolution of genes on the t-haplotype,\r\nand finds widespread expression and copy number changes and signs of less efficient purifying\r\nselection compared to the genes on the non-driving homolog. Further, this chapter finds\r\ncandidates for involvment in drive: two positively selected genes on the t-haplotype, and\r\nthe discovery of a t-specific gene duplicate, which was gained from another chromosome,\r\nand which acquired novel sequence and testis-specific expression on the t-haplotype. Finally,\r\nChapter 4 provides unprecedented insights into the gene expression landscape in testes of\r\nt-carrier mice, using single nucleus sequencing. Cell-resolved RNA-sequencing allows the\r\ncomparison of expression in spermatids carrying or not carrying the t-haplotype as well as the\r\ntiming of t-haplotype-induced expression changes along spermatogenesis. This study shows\r\nthe timing of previously found drive-associated genes, and uncovers novel candidate genes and\r\nbiological processes that may underlie the complex biology of transmission ratio distortion of\r\nthe t-haplotype. Chapter 5 synthesizes the findings of the three studies, and discusses them in\r\nthe context of the current state of meiotic drive research."}],"type":"dissertation","keyword":["meiotic driver","neofunctionalization","single nucleus sequencing"],"file":[{"relation":"source_file","access_level":"closed","creator":"rkelemen","date_updated":"2025-01-10T23:30:10Z","checksum":"fab59146e3b3dc2e5d214576984a2a63","file_size":180557931,"file_id":"17121","file_name":"thesis.zip","content_type":"application/zip","date_created":"2024-06-07T16:09:17Z","embargo_to":"open_access"},{"date_created":"2024-07-10T08:00:20Z","content_type":"application/pdf","file_name":"thesis_to_archive.pdf","embargo":"2025-01-10","file_size":19405484,"checksum":"91cc4c25a792239e8a7688e8aec7c62a","file_id":"17213","relation":"main_file","date_updated":"2025-01-10T23:30:10Z","creator":"rkelemen","access_level":"open_access"}],"oa":1,"corr_author":"1","status":"public","publication_identifier":{"isbn":["978-3-99078-039-8"],"issn":["2663-337X"]},"citation":{"short":"R.K. Kelemen, Characterizing the Sequence and Expression Evolution of the T-Haplotype, a Model Meiotic Driver, Institute of Science and Technology Austria, 2024.","ista":"Kelemen RK. 2024. Characterizing the sequence and expression evolution of the t-haplotype, a model meiotic driver. Institute of Science and Technology Austria.","mla":"Kelemen, Réka K. <i>Characterizing the Sequence and Expression Evolution of the T-Haplotype, a Model Meiotic Driver</i>. Institute of Science and Technology Austria, 2024, doi:<a href=\"https://doi.org/10.15479/at:ista:17119\">10.15479/at:ista:17119</a>.","ama":"Kelemen RK. Characterizing the sequence and expression evolution of the t-haplotype, a model meiotic driver. 2024. doi:<a href=\"https://doi.org/10.15479/at:ista:17119\">10.15479/at:ista:17119</a>","apa":"Kelemen, R. K. (2024). <i>Characterizing the sequence and expression evolution of the t-haplotype, a model meiotic driver</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:17119\">https://doi.org/10.15479/at:ista:17119</a>","ieee":"R. K. Kelemen, “Characterizing the sequence and expression evolution of the t-haplotype, a model meiotic driver,” Institute of Science and Technology Austria, 2024.","chicago":"Kelemen, Réka K. “Characterizing the Sequence and Expression Evolution of the T-Haplotype, a Model Meiotic Driver.” Institute of Science and Technology Austria, 2024. <a href=\"https://doi.org/10.15479/at:ista:17119\">https://doi.org/10.15479/at:ista:17119</a>."},"department":[{"_id":"GradSch"},{"_id":"BeVi"}],"OA_place":"publisher","language":[{"iso":"eng"}],"has_accepted_license":"1","_id":"17119","date_updated":"2026-04-07T13:21:37Z","article_processing_charge":"No","file_date_updated":"2025-01-10T23:30:10Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"supervisor":[{"full_name":"Vicoso, Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4579-8306","first_name":"Beatriz","last_name":"Vicoso"}],"alternative_title":["ISTA Thesis"],"publication_status":"published","publisher":"Institute of Science and Technology Austria","date_published":"2024-06-20T00:00:00Z","ec_funded":1,"oa_version":"Published Version"},{"pmid":1,"department":[{"_id":"BeVi"}],"publication_identifier":{"eissn":["1553-7404"],"issn":["1553-7390"]},"citation":{"ieee":"M. N. Elkrewi and B. Vicoso, “Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome,” <i>PLoS Genetics</i>, vol. 20, no. 8. Public Library of Science, 2024.","chicago":"Elkrewi, Marwan N, and Beatriz Vicoso. “Single-Nucleus Atlas of the Artemia Female Reproductive System Suggests Germline Repression of the Z Chromosome.” <i>PLoS Genetics</i>. Public Library of Science, 2024. <a href=\"https://doi.org/10.1371/journal.pgen.1011376\">https://doi.org/10.1371/journal.pgen.1011376</a>.","apa":"Elkrewi, M. N., &#38; Vicoso, B. (2024). Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1011376\">https://doi.org/10.1371/journal.pgen.1011376</a>","ista":"Elkrewi MN, Vicoso B. 2024. Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome. PLoS Genetics. 20(8), e1011376.","short":"M.N. Elkrewi, B. Vicoso, PLoS Genetics 20 (2024).","mla":"Elkrewi, Marwan N., and Beatriz Vicoso. “Single-Nucleus Atlas of the Artemia Female Reproductive System Suggests Germline Repression of the Z Chromosome.” <i>PLoS Genetics</i>, vol. 20, no. 8, e1011376, Public Library of Science, 2024, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1011376\">10.1371/journal.pgen.1011376</a>.","ama":"Elkrewi MN, Vicoso B. Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome. <i>PLoS Genetics</i>. 2024;20(8). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1011376\">10.1371/journal.pgen.1011376</a>"},"external_id":{"isi":["001304090200001"],"pmid":["39213449"]},"has_accepted_license":"1","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"gold","issue":"8","_id":"17890","article_processing_charge":"Yes","date_updated":"2026-04-30T22:31:07Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"        20","file_date_updated":"2024-09-11T07:54:12Z","publication":"PLoS Genetics","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"isi":1,"acknowledgement":"We thank the Vicoso group for their valuable comments on the earlier draft of the manuscript. We would also like to thank the Vienna BioCenter Next Generation Sequencing (NGS) facility staff, and in particular, Thomas Grentzinger for his support with the handling and sequencing of the samples, the scientific computing unit at ISTA for the computational resources, Brittney Wick for the help with hosting our data on the UCSC Cell Browser, and Lora B. Sweeney for her valuable input at the different stages of the project.\r\nThis research was funded by the Austrian science fund (FWF), as part of the SFB Meiosis consortium https://sfbmeiosis.org/, grant ID FWF SFB F88-10) to BV. ","APC_amount":"3145,39 EUR","publisher":"Public Library of Science","publication_status":"published","volume":20,"date_published":"2024-08-30T00:00:00Z","oa_version":"Published Version","scopus_import":"1","day":"30","DOAJ_listed":"1","doi":"10.1371/journal.pgen.1011376","year":"2024","project":[{"name":"FWF Open Access Fund","_id":"3AC91DDA-15DF-11EA-824D-93A3E7B544D1","call_identifier":"FWF"},{"grant_number":"F8810","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","name":"The highjacking of meiosis for asexual reproduction"}],"author":[{"full_name":"Elkrewi, Marwan N","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","orcid":"0000-0002-5328-7231","first_name":"Marwan N","last_name":"Elkrewi"},{"orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","full_name":"Vicoso, Beatriz"}],"article_type":"original","article_number":"e1011376","related_material":{"record":[{"status":"public","relation":"research_data","id":"17362"},{"status":"public","relation":"dissertation_contains","id":"19386"}],"link":[{"relation":"software","url":"https://github.com/Melkrewi/Artemia-snRNAseq-Project"}]},"ddc":["570"],"title":"Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome","month":"08","date_created":"2024-09-08T22:01:11Z","abstract":[{"lang":"eng","text":"Our understanding of the molecular pathways that regulate oogenesis and define cellular identity in the Arthropod female reproductive system and the extent of their conservation is currently very limited. This is due to the focus on model systems, including Drosophila and Daphnia, which do not reflect the observed diversity of morphologies, reproductive modes, and sex chromosome systems. We use single-nucleus RNA and ATAC sequencing to produce a comprehensive single nucleus atlas of the adult Artemia franciscana female reproductive system. We map our data to the Fly Cell Atlas single-nucleus dataset of the Drosophila melanogaster ovary, shedding light on the conserved regulatory programs between the two distantly related Arthropod species. We identify the major cell types known to be present in the Artemia ovary, including germ cells, follicle cells, and ovarian muscle cells. Additionally, we use the germ cells to explore gene regulation and expression of the Z chromosome during meiosis, highlighting its unique regulatory dynamics and allowing us to explore the presence of meiotic sex chromosome silencing in this group."}],"type":"journal_article","file":[{"relation":"main_file","access_level":"open_access","creator":"dernst","date_updated":"2024-09-11T07:54:12Z","checksum":"f5d96b9af57126fc1063e951440477d6","file_size":8962687,"file_id":"18056","file_name":"2024_PloSGenetics_Elkrewi.pdf","content_type":"application/pdf","date_created":"2024-09-11T07:54:12Z","success":1}],"acknowledged_ssus":[{"_id":"ScienComp"}],"oa":1,"corr_author":"1","status":"public","quality_controlled":"1"},{"day":"01","scopus_import":"1","project":[{"grant_number":"F8810","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","name":"The highjacking of meiosis for asexual reproduction"},{"name":"Mechanisms and Evolution of Reproductive Plasticity","_id":"ebb230e0-77a9-11ec-83b8-87a37e0241d3","grant_number":"ESP39 49461"}],"author":[{"full_name":"Lasne, Clementine","id":"02225f57-50d2-11eb-9ed8-8c92b9a34237","orcid":"0000-0002-1197-8616","first_name":"Clementine","last_name":"Lasne"},{"full_name":"Elkrewi, Marwan N","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","first_name":"Marwan N","last_name":"Elkrewi","orcid":"0000-0002-5328-7231"},{"orcid":"0000-0002-9752-7380","first_name":"Melissa A","last_name":"Toups","id":"4E099E4E-F248-11E8-B48F-1D18A9856A87","full_name":"Toups, Melissa A"},{"first_name":"Lorena Alexandra","last_name":"Layana Franco","orcid":"0000-0002-1253-6297","id":"02814589-eb8f-11eb-b029-a70074f3f18f","full_name":"Layana Franco, Lorena Alexandra"},{"full_name":"Macon, Ariana","id":"2A0848E2-F248-11E8-B48F-1D18A9856A87","last_name":"Macon","first_name":"Ariana"},{"orcid":"0000-0002-4579-8306","last_name":"Vicoso","first_name":"Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","full_name":"Vicoso, Beatriz"}],"doi":"10.1093/molbev/msad245","year":"2023","article_type":"original","article_number":"msad245","related_material":{"record":[{"id":"14614","status":"public","relation":"research_data"},{"id":"19386","relation":"dissertation_contains","status":"public"}],"link":[{"description":"News on ISTA webpage","url":"https://ista.ac.at/en/news/on-the-hunt/","relation":"press_release"}]},"ddc":["570"],"title":"The scorpionfly (Panorpa cognata) genome highlights conserved and derived features of the peculiar dipteran X chromosome","month":"12","date_created":"2023-11-27T16:14:37Z","abstract":[{"lang":"eng","text":"Many insects carry an ancient X chromosome - the Drosophila Muller element F - that likely predates their origin. Interestingly, the X has undergone turnover in multiple fly species (Diptera) after being conserved for more than 450 MY. The long evolutionary distance between Diptera and other sequenced insect clades makes it difficult to infer what could have contributed to this sudden increase in rate of turnover. Here, we produce the first genome and transcriptome of a long overlooked sister-order to Diptera: Mecoptera. We compare the scorpionfly Panorpa cognata X-chromosome gene content, expression, and structure, to that of several dipteran species as well as more distantly-related insect orders (Orthoptera and Blattodea). We find high conservation of gene content between the mecopteran X and the dipteran Muller F element, as well as several shared biological features, such as the presence of dosage compensation and a low amount of genetic diversity, consistent with a low recombination rate. However, the two homologous X chromosomes differ strikingly in their size and number of genes they carry. Our results therefore support a common ancestry of the mecopteran and ancestral dipteran X chromosomes, and suggest that Muller element F shrank in size and gene content after the split of Diptera and Mecoptera, which may have contributed to its turnover in dipteran insects."}],"type":"journal_article","keyword":["Genetics","Molecular Biology","Ecology","Evolution","Behavior and Systematics"],"oa":1,"acknowledged_ssus":[{"_id":"ScienComp"}],"file":[{"file_name":"2023_MolecularBioEvo_Lasne.pdf","content_type":"application/pdf","date_created":"2024-01-02T11:39:38Z","success":1,"relation":"main_file","creator":"dernst","access_level":"open_access","date_updated":"2024-01-02T11:39:38Z","checksum":"47c1c72fb499f26ea52d216b242208c8","file_size":8623505,"file_id":"14727"}],"corr_author":"1","status":"public","quality_controlled":"1","publication_identifier":{"eissn":["1537-1719"],"issn":["0737-4038"]},"citation":{"apa":"Lasne, C., Elkrewi, M. N., Toups, M. A., Layana Franco, L. A., Macon, A., &#38; Vicoso, B. (2023). The scorpionfly (Panorpa cognata) genome highlights conserved and derived features of the peculiar dipteran X chromosome. <i>Molecular Biology and Evolution</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/molbev/msad245\">https://doi.org/10.1093/molbev/msad245</a>","short":"C. Lasne, M.N. Elkrewi, M.A. Toups, L.A. Layana Franco, A. Macon, B. Vicoso, Molecular Biology and Evolution 40 (2023).","ista":"Lasne C, Elkrewi MN, Toups MA, Layana Franco LA, Macon A, Vicoso B. 2023. The scorpionfly (Panorpa cognata) genome highlights conserved and derived features of the peculiar dipteran X chromosome. Molecular Biology and Evolution. 40(12), msad245.","mla":"Lasne, Clementine, et al. “The Scorpionfly (Panorpa Cognata) Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.” <i>Molecular Biology and Evolution</i>, vol. 40, no. 12, msad245, Oxford University Press, 2023, doi:<a href=\"https://doi.org/10.1093/molbev/msad245\">10.1093/molbev/msad245</a>.","ama":"Lasne C, Elkrewi MN, Toups MA, Layana Franco LA, Macon A, Vicoso B. The scorpionfly (Panorpa cognata) genome highlights conserved and derived features of the peculiar dipteran X chromosome. <i>Molecular Biology and Evolution</i>. 2023;40(12). doi:<a href=\"https://doi.org/10.1093/molbev/msad245\">10.1093/molbev/msad245</a>","ieee":"C. Lasne, M. N. Elkrewi, M. A. Toups, L. A. Layana Franco, A. Macon, and B. Vicoso, “The scorpionfly (Panorpa cognata) genome highlights conserved and derived features of the peculiar dipteran X chromosome,” <i>Molecular Biology and Evolution</i>, vol. 40, no. 12. Oxford University Press, 2023.","chicago":"Lasne, Clementine, Marwan N Elkrewi, Melissa A Toups, Lorena Alexandra Layana Franco, Ariana Macon, and Beatriz Vicoso. “The Scorpionfly (Panorpa Cognata) Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.” <i>Molecular Biology and Evolution</i>. Oxford University Press, 2023. <a href=\"https://doi.org/10.1093/molbev/msad245\">https://doi.org/10.1093/molbev/msad245</a>."},"pmid":1,"department":[{"_id":"BeVi"}],"external_id":{"isi":["001122489000003"],"pmid":["37988296"]},"language":[{"iso":"eng"}],"has_accepted_license":"1","issue":"12","_id":"14613","date_updated":"2026-04-30T22:31:07Z","article_processing_charge":"Yes","file_date_updated":"2024-01-02T11:39:38Z","intvolume":"        40","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication":"Molecular Biology and Evolution","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"acknowledgement":"We thank the Vicoso lab for their assistance with specimen collection, and Tim Connallon for valuable comments and suggestions on earlier versions of the manuscript. Computational resources and support were provided by the Scientific Computing unit at the ISTA. This research was supported by grants from the Austrian Science Foundation to C.L.\r\n(FWF ESP 39), and to B.V. (FWF SFB F88-10).","isi":1,"publication_status":"published","publisher":"Oxford University Press","volume":40,"date_published":"2023-12-01T00:00:00Z","oa_version":"Published Version"},{"publication_status":"published","publisher":"Oxford University Press","ec_funded":1,"oa_version":"Published Version","date_published":"2022-10-01T00:00:00Z","volume":222,"publication":"Genetics","file_date_updated":"2023-01-30T08:59:58Z","intvolume":"       222","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 715257) and by the Austrian Science Foundation (FWF SFB F88-10).\r\nWe thank the Vicoso group for comments on the manuscript and the ISTA Scientific computing team and the Vienna Biocenter Sequencing facility for technical support.","isi":1,"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"issue":"2","date_updated":"2026-04-30T22:31:07Z","article_processing_charge":"No","_id":"12248","external_id":{"isi":["000850270300001"],"pmid":["35977389"]},"citation":{"apa":"Elkrewi, M. N., Khauratovich, U., Toups, M. A., Bett, V. K., Mrnjavac, A., Macon, A., … Vicoso, B. (2022). ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp. <i>Genetics</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/genetics/iyac123\">https://doi.org/10.1093/genetics/iyac123</a>","short":"M.N. Elkrewi, U. Khauratovich, M.A. Toups, V.K. Bett, A. Mrnjavac, A. Macon, C. Fraisse, L. Sax, A.K. Huylmans, F. Hontoria, B. Vicoso, Genetics 222 (2022).","ista":"Elkrewi MN, Khauratovich U, Toups MA, Bett VK, Mrnjavac A, Macon A, Fraisse C, Sax L, Huylmans AK, Hontoria F, Vicoso B. 2022. ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp. Genetics. 222(2), iyac123.","mla":"Elkrewi, Marwan N., et al. “ZW Sex-Chromosome Evolution and Contagious Parthenogenesis in Artemia Brine Shrimp.” <i>Genetics</i>, vol. 222, no. 2, iyac123, Oxford University Press, 2022, doi:<a href=\"https://doi.org/10.1093/genetics/iyac123\">10.1093/genetics/iyac123</a>.","ama":"Elkrewi MN, Khauratovich U, Toups MA, et al. ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp. <i>Genetics</i>. 2022;222(2). doi:<a href=\"https://doi.org/10.1093/genetics/iyac123\">10.1093/genetics/iyac123</a>","ieee":"M. N. Elkrewi <i>et al.</i>, “ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp,” <i>Genetics</i>, vol. 222, no. 2. Oxford University Press, 2022.","chicago":"Elkrewi, Marwan N, Uladzislava Khauratovich, Melissa A Toups, Vincent K Bett, Andrea Mrnjavac, Ariana Macon, Christelle Fraisse, et al. “ZW Sex-Chromosome Evolution and Contagious Parthenogenesis in Artemia Brine Shrimp.” <i>Genetics</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/genetics/iyac123\">https://doi.org/10.1093/genetics/iyac123</a>."},"publication_identifier":{"issn":["1943-2631"]},"pmid":1,"department":[{"_id":"BeVi"}],"language":[{"iso":"eng"}],"has_accepted_license":"1","corr_author":"1","quality_controlled":"1","status":"public","abstract":[{"text":"Eurasian brine shrimp (genus Artemia) have closely related sexual and asexual lineages of parthenogenetic females, which produce rare males at low frequencies. Although they are known to have ZW chromosomes, these are not well characterized, and it is unclear whether they are shared across the clade. Furthermore, the underlying genetic architecture of the transmission of asexuality, which can occur when rare males mate with closely related sexual females, is not well understood. We produced a chromosome-level assembly for the sexual Eurasian species Artemia sinica and characterized in detail the pair of sex chromosomes of this species. We combined this new assembly with short-read genomic data for the sexual species Artemia sp. Kazakhstan and several asexual lineages of Artemia parthenogenetica, allowing us to perform an in-depth characterization of sex-chromosome evolution across the genus. We identified a small differentiated region of the ZW pair that is shared by all sexual and asexual lineages, supporting the shared ancestry of the sex chromosomes. We also inferred that recombination suppression has spread to larger sections of the chromosome independently in the American and Eurasian lineages. Finally, we took advantage of a rare male, which we backcrossed to sexual females, to explore the genetic basis of asexuality. Our results suggest that parthenogenesis is likely partly controlled by a locus on the Z chromosome, highlighting the interplay between sex determination and asexuality.","lang":"eng"}],"date_created":"2023-01-16T09:56:10Z","keyword":["Genetics"],"file":[{"content_type":"application/pdf","date_created":"2023-01-30T08:59:58Z","file_name":"2022_Genetics_Elkrewi.pdf","success":1,"relation":"main_file","date_updated":"2023-01-30T08:59:58Z","creator":"dernst","access_level":"open_access","file_size":1347136,"checksum":"f79ff5383e882ea3f95f3da47a78029d","file_id":"12440"}],"oa":1,"acknowledged_ssus":[{"_id":"ScienComp"}],"type":"journal_article","ddc":["570"],"related_material":{"record":[{"id":"11653","status":"public","relation":"research_data"},{"id":"19386","status":"public","relation":"dissertation_contains"}]},"month":"10","title":"ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp","author":[{"last_name":"Elkrewi","first_name":"Marwan N","orcid":"0000-0002-5328-7231","id":"0B46FACA-A8E1-11E9-9BD3-79D1E5697425","full_name":"Elkrewi, Marwan N"},{"full_name":"Khauratovich, Uladzislava","id":"5eba06f4-97d8-11ed-9f8f-d826ebdd9434","first_name":"Uladzislava","last_name":"Khauratovich"},{"full_name":"Toups, Melissa A","id":"4E099E4E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9752-7380","first_name":"Melissa A","last_name":"Toups"},{"id":"57854184-AAE0-11E9-8D04-98D6E5697425","full_name":"Bett, Vincent K","last_name":"Bett","first_name":"Vincent K"},{"id":"353FAC84-AE61-11E9-8BFC-00D3E5697425","full_name":"Mrnjavac, Andrea","last_name":"Mrnjavac","first_name":"Andrea"},{"full_name":"Macon, Ariana","id":"2A0848E2-F248-11E8-B48F-1D18A9856A87","last_name":"Macon","first_name":"Ariana"},{"id":"32DF5794-F248-11E8-B48F-1D18A9856A87","full_name":"Fraisse, Christelle","last_name":"Fraisse","first_name":"Christelle","orcid":"0000-0001-8441-5075"},{"full_name":"Sax, Luca","id":"701c5602-97d8-11ed-96b5-b52773c70189","last_name":"Sax","first_name":"Luca"},{"first_name":"Ann K","last_name":"Huylmans","orcid":"0000-0001-8871-4961","id":"4C0A3874-F248-11E8-B48F-1D18A9856A87","full_name":"Huylmans, Ann K"},{"first_name":"Francisco","last_name":"Hontoria","full_name":"Hontoria, Francisco"},{"full_name":"Vicoso, Beatriz","id":"49E1C5C6-F248-11E8-B48F-1D18A9856A87","last_name":"Vicoso","first_name":"Beatriz","orcid":"0000-0002-4579-8306"}],"project":[{"name":"Prevalence and Influence of Sexual Antagonism on Genome Evolution","call_identifier":"H2020","grant_number":"715257","_id":"250BDE62-B435-11E9-9278-68D0E5697425"},{"name":"The highjacking of meiosis for asexual reproduction","_id":"34ae1506-11ca-11ed-8bc3-c14f4c474396","grant_number":"F8810"}],"year":"2022","doi":"10.1093/genetics/iyac123","day":"01","scopus_import":"1","article_number":"iyac123","article_type":"original"}]