[{"oa_version":"Preprint","day":"30","citation":{"ieee":"Z. Ge <i>et al.</i>, “MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis,” <i>bioRxiv</i>. .","ama":"Ge Z, Koczka L, Mazur E, et al. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.07.680881\">10.1101/2025.10.07.680881</a>","apa":"Ge, Z., Koczka, L., Mazur, E., Molnar, G., Vladimirtsev, D., Kassem, N., … Friml, J. (n.d.). MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.07.680881\">https://doi.org/10.1101/2025.10.07.680881</a>","short":"Z. Ge, L. Koczka, E. Mazur, G. Molnar, D. Vladimirtsev, N. Kassem, S. Ait Ikene, L. Fiedler, J. Friml, BioRxiv (n.d.).","chicago":"Ge, Zengxiang, Lilla Koczka, Ewa Mazur, Gergely Molnar, Dmitrii Vladimirtsev, Nada Kassem, Sara Ait Ikene, Lukas Fiedler, and Jiří Friml. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.07.680881\">https://doi.org/10.1101/2025.10.07.680881</a>.","mla":"Ge, Zengxiang, et al. “MAKR6 Integrates TMK and CAMEL/CANAR Signalling for Auxin Canalization in Arabidopsis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.07.680881\">10.1101/2025.10.07.680881</a>.","ista":"Ge Z, Koczka L, Mazur E, Molnar G, Vladimirtsev D, Kassem N, Ait Ikene S, Fiedler L, Friml J. MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.07.680881\">10.1101/2025.10.07.680881</a>."},"has_accepted_license":"1","publication":"bioRxiv","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"article_processing_charge":"No","type":"preprint","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"year":"2026","language":[{"iso":"eng"}],"publication_status":"submitted","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1101/2025.10.07.680881","date_created":"2026-06-13T16:57:07Z","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","OA_place":"repository","abstract":[{"lang":"eng","text":"Adaptive plant development is orchestrated, among others, by directional, intercellular transport of the phytohormone auxin. Self-organizing development, such as flexible vasculature formation, depends on so-called auxin canalization, manifested by the gradual formation of auxin transport channels through feedback between auxin signalling and transport. Herein, we identify MAKR6 as an important, novel component in this feedback. MAKR6 expression accumulates strongly in vascular cells and is tightly regulated by auxin via the Aux/IAA-ARF-WRKY23 transcriptional network. MAKR6 is required for auxin canalization-dependent processes, including leaf venation, vasculature regeneration, and de novo auxin channel formation from local auxin sources. Mechanistically, MAKR6 interacts with the PIN1 auxin transporter, modulating its trafficking and polarization. MAKR6 also associates with and integrates two key receptor-like kinase complexes involved in canalization, TMK1/4 and the CAMEL-CANAR. Together, our study establishes MAKR6 as a multifaceted regulator that couples transcriptional auxin signalling to PIN1 repolarization and coordinates multiple RLK-mediated signalling pathways during canalization. This provides mechanistic insights into auxin canalization and exemplifies a framework for exploring similar regulatory nodes in other developmental contexts."}],"fulldoi":"https://doi.org/10.1101/2025.10.07.680881","acknowledgement":"We would like to thank Dr. Yvon Jaillais (ENS, Lyon) for sharing MAKR2 materials. This research was supported by the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The research in the Friml group leading to these results was funded by the European Research Council (ERC): 101142681 CYNIPS; and the Austrian Science Fund (FWF): I 6123-B and P 37051-B. Ewa Mazur was supported by the National Science Centre (NCN), Poland, under the OPUS call in the WEAVE programme: 2021/43/I/NZ1/01835.","main_file_link":[{"url":"https://doi.org/10.1101/2025.10.07.680881","open_access":"1"}],"ddc":["580"],"author":[{"orcid":"0000-0001-9381-3577","first_name":"Zengxiang","full_name":"Ge, Zengxiang","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","last_name":"Ge"},{"first_name":"Lilla","full_name":"Koczka, Lilla","last_name":"Koczka"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"last_name":"Molnar","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","full_name":"Molnar, Gergely","first_name":"Gergely"},{"first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d"},{"last_name":"Kassem","full_name":"Kassem, Nada","first_name":"Nada"},{"id":"6a0bb896-6bad-11f1-9bef-906e9eb76034","last_name":"Ait Ikene","full_name":"Ait Ikene, Sara","first_name":"Sara"},{"first_name":"Lukas","last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986","full_name":"Fiedler, Lukas"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"}],"OA_type":"green","status":"public","title":"MAKR6 integrates TMK and CAMEL/CANAR signalling for auxin canalization in Arabidopsis","oa":1,"project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"},{"grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"month":"05","scopus_import":"1","date_published":"2026-05-30T00:00:00Z","_id":"21994","corr_author":"1","date_updated":"2026-06-19T07:14:01Z"},{"author":[{"id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich","full_name":"Kulich, Ivan","first_name":"Ivan"},{"first_name":"Dmitrii","last_name":"Vladimirtsev","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","full_name":"Vladimirtsev, Dmitrii"},{"last_name":"Randuch","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek","first_name":"Marek"},{"last_name":"Gao","full_name":"Gao, Shiqiang","first_name":"Shiqiang"},{"full_name":"Citterico, Matteo","last_name":"Citterico","first_name":"Matteo"},{"full_name":"Konrad, Kai R.","last_name":"Konrad","first_name":"Kai R."},{"first_name":"Georg","last_name":"Nagel","full_name":"Nagel, Georg"},{"first_name":"Michael","last_name":"Wrzaczek","full_name":"Wrzaczek, Michael"},{"first_name":"Léa","last_name":"Cascaro","full_name":"Cascaro, Léa"},{"last_name":"Vinet","full_name":"Vinet, Pauline","first_name":"Pauline"},{"first_name":"Pauline","last_name":"Durand","full_name":"Durand, Pauline"},{"last_name":"Asnacios","full_name":"Asnacios, Atef","first_name":"Atef"},{"full_name":"Verma, Lokesh","last_name":"Verma","first_name":"Lokesh"},{"last_name":"Bennett","full_name":"Bennett, Malcolm J.","first_name":"Malcolm J."},{"full_name":"Pandey, Bipin K.","last_name":"Pandey","first_name":"Bipin K."},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"OA_type":"green","pmid":1,"ddc":["580"],"abstract":[{"text":"Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil.","lang":"eng"}],"article_type":"original","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"acknowledgement":"We gratefully acknowledge the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) (both of ISTA) and the Hounsfield CT Facility (University of Nottingham) for support with imaging and the Growth Facility (IPMB) for plant cultivation. We thank M. Fendrych and his team for help with the microfluidics upgrades and J. Atkinson at the University of Nottingham MakerSpace for 3D printing of Arabidopsis mini-soil columns.\r\nThis project received funding from the European Research Council (ERC; 101142681 CYNIPS) and the Austrian Science Fund (FWF; P 37051-B). I.K. was cofunded by the European Union, Horizon Europe, project MOLIPEC, ID 101087030 and CSF project 25-16449S. L.V. and B.K.P. acknowledge funding from UK Research and Innovation (UKRI) Frontiers Research (EP/Y036697/1). M.J.B. acknowledges funding from ERC SYNERGY (grant 101118769 HYDROSENSING). The study was partially supported by the Université Paris Cité, Idex ANR-18-IDEX-0001, funded by the French Government through its “Investments for the Future” program and also by the projects “Mecha-Nuc” ANR-20-CE13-0025-03 and “scEm-bryoMech” ANR-21-CE13-0046. P.D. acknowledges support by Human Frontier Science Program Organization grant 2022-RG107. P.V. acknowledges support provided by “Programme blanc” of the Graduate School BIOSPHERA, Université Paris-Saclay. Phytohormonal analysis was performed using the service laboratory funded by Toward Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the European Regional Development Fund (ERDF) program Johannes Amos Comenius. This research was funded in whole or in part by the Austrian Science Fund (P 37051-B) and UK Research and Innovation (EP/Y036697/1), cOAlition S organizations, and by the European Research Council (101142681 CYNIPS, 101118769 HYDROSENSING); as required, the author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.","file":[{"date_updated":"2026-05-07T05:54:43Z","success":1,"file_size":6150733,"checksum":"eb5b29247832ecdc53c8146da0509bbe","file_id":"21832","access_level":"open_access","content_type":"application/pdf","creator":"dernst","date_created":"2026-05-07T05:54:43Z","file_name":"2026_Science_Kulich_accepted.pdf","relation":"main_file"}],"fulldoi":"https://doi.org/10.1126/science.adu8197","date_created":"2026-04-26T22:01:47Z","OA_place":"repository","date_updated":"2026-05-07T06:20:07Z","quality_controlled":"1","corr_author":"1","date_published":"2026-04-16T00:00:00Z","scopus_import":"1","_id":"21763","month":"04","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"title":"Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation","status":"public","volume":392,"file_date_updated":"2026-05-07T05:54:43Z","page":"296-300","oa":1,"external_id":{"pmid":["41990180"]},"oa_version":"Accepted Version","day":"16","intvolume":"       392","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"doi":"10.1126/science.adu8197","publisher":"AAAS","type":"journal_article","issue":"6795","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"year":"2026","has_accepted_license":"1","article_processing_charge":"No","publication":"Science","citation":{"ista":"Kulich I, Vladimirtsev D, Randuch M, Gao S, Citterico M, Konrad KR, Nagel G, Wrzaczek M, Cascaro L, Vinet P, Durand P, Asnacios A, Verma L, Bennett MJ, Pandey BK, Friml J. 2026. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. Science. 392(6795), 296–300.","short":"I. Kulich, D. Vladimirtsev, M. Randuch, S. Gao, M. Citterico, K.R. Konrad, G. Nagel, M. Wrzaczek, L. Cascaro, P. Vinet, P. Durand, A. Asnacios, L. Verma, M.J. Bennett, B.K. Pandey, J. Friml, Science 392 (2026) 296–300.","apa":"Kulich, I., Vladimirtsev, D., Randuch, M., Gao, S., Citterico, M., Konrad, K. R., … Friml, J. (2026). Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>","chicago":"Kulich, Ivan, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/science.adu8197\">https://doi.org/10.1126/science.adu8197</a>.","ama":"Kulich I, Vladimirtsev D, Randuch M, et al. Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation. <i>Science</i>. 2026;392(6795):296-300. doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>","ieee":"I. Kulich <i>et al.</i>, “Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation,” <i>Science</i>, vol. 392, no. 6795. AAAS, pp. 296–300, 2026.","mla":"Kulich, Ivan, et al. “Calcium-Triggered Apoplastic ROS Bursts Balance Gravity and Mechanical Signals for Soil Navigation.” <i>Science</i>, vol. 392, no. 6795, AAAS, 2026, pp. 296–300, doi:<a href=\"https://doi.org/10.1126/science.adu8197\">10.1126/science.adu8197</a>."}},{"OA_type":"gold","author":[{"first_name":"Mingyue","full_name":"Li, Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","last_name":"Li"},{"full_name":"Chodasiewicz, Monika","last_name":"Chodasiewicz","first_name":"Monika"},{"first_name":"Malavika","last_name":"Muraleedharan","full_name":"Muraleedharan, Malavika"},{"first_name":"Israel M.","last_name":"Lopez","full_name":"Lopez, Israel M."},{"first_name":"Michal","full_name":"Gorka, Michal","last_name":"Gorka"},{"first_name":"Olga","full_name":"Kerber, Olga","last_name":"Kerber"},{"last_name":"Alotaibi","full_name":"Alotaibi, Saqer S.","first_name":"Saqer S."},{"first_name":"Andrew D.L.","full_name":"Nelson, Andrew D.L.","last_name":"Nelson"},{"first_name":"Rene","last_name":"Lenobel","full_name":"Lenobel, Rene"},{"full_name":"Friedecká, Jaroslava","last_name":"Friedecká","first_name":"Jaroslava"},{"first_name":"Aleksandra","last_name":"Skirycz","full_name":"Skirycz, Aleksandra"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"}],"pmid":1,"ddc":["580"],"fulldoi":"https://doi.org/10.1126/sciadv.aea7828","file":[{"date_updated":"2026-06-02T14:33:55Z","success":1,"checksum":"75b8ef2db078652c750e34e9cd98a808","file_size":2014452,"access_level":"open_access","file_id":"21941","creator":"dernst","content_type":"application/pdf","date_created":"2026-06-02T14:33:55Z","file_name":"2026_ScienceAdv_Li2.pdf","relation":"main_file"}],"acknowledgement":" We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid and K. Jaworski (Nicolaus Copernicus University) for the GST-­HpAC1 plasmid. We also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5 and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute of Science and Technology Austria for their contributions,\r\nincluding consultation on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis, and absolute quantification. This project is supported by the European\r\nResearch Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram (101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe acknowledge the generous support of the Taif University Researchers Supporting\r\nProject: TURSP-­HC2022/02 and Max-Planck-Society to A.S. ","article_type":"original","publication_identifier":{"eissn":["2375-2548"]},"article_number":"aea7828","abstract":[{"lang":"eng","text":"Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved in diverse signaling pathways across both animals and plants. While the role of 3′,5′-cAMP has been extensively characterized, the biological significance of its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling systems in Arabidopsis thaliana, with different enzymatic origins and largely distinct downstream effects. In vitro enzymatic assays show that plant adenylate cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct yet partially overlapping metabolic, proteomic, and transcriptional response: 2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while 3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis. Our findings establish the existence of dual cAMP signaling systems in plants, each with specialized functions and provide insights into the complex regulatory networks governing plant physiology."}],"OA_place":"publisher","date_created":"2026-05-24T22:01:31Z","corr_author":"1","quality_controlled":"1","DOAJ_listed":"1","date_updated":"2026-06-02T14:36:41Z","_id":"21914","scopus_import":"1","date_published":"2026-05-08T00:00:00Z","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"month":"05","oa":1,"file_date_updated":"2026-06-02T14:33:55Z","status":"public","volume":12,"title":"Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants","external_id":{"pmid":["42102187"]},"day":"08","oa_version":"Published Version","PlanS_conform":"1","intvolume":"        12","publisher":"AAAS","doi":"10.1126/sciadv.aea7828","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JiFr"}],"issue":"19","year":"2026","type":"journal_article","publication":"Science Advances","article_processing_charge":"Yes","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","citation":{"ista":"Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 12(19), aea7828.","mla":"Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>, vol. 12, no. 19, aea7828, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>.","ieee":"M. Li <i>et al.</i>, “Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants,” <i>Science Advances</i>, vol. 12, no. 19. AAAS, 2026.","ama":"Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. 2026;12(19). doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>","apa":"Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber, O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>","chicago":"Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>.","short":"M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber, S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml, Science Advances 12 (2026)."}},{"date_updated":"2026-07-13T14:26:31Z","quality_controlled":"1","corr_author":"1","date_published":"2026-06-10T00:00:00Z","scopus_import":"1","_id":"22301","month":"06","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"title":"Auxin response and PIN‐mediated transport in chlorophyte algae","status":"public","oa":1,"supplementarymaterial":"yes","author":[{"first_name":"Adrijana","last_name":"Smoljan","id":"cced8a85-223e-11ed-af04-b0596c55053b","full_name":"Smoljan, Adrijana"},{"last_name":"Koutnik‐Abele","full_name":"Koutnik‐Abele, Sarah","first_name":"Sarah"},{"first_name":"Dmitrii","full_name":"Vladimirtsev, Dmitrii","id":"60466724-5355-11ee-ae5a-fa55e8f99c3d","last_name":"Vladimirtsev"},{"last_name":"Klíma","full_name":"Klíma, Petr","first_name":"Petr"},{"full_name":"Bírošíková, Anita","last_name":"Bírošíková","first_name":"Anita"},{"orcid":"0000-0003-2627-6956","first_name":"Yuzhou","full_name":"Zhang, Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","orcid":"0000-0001-5145-4609","first_name":"Jack"},{"full_name":"Schuster, Maximilian","id":"37e65def-d415-11eb-ae59-a7b67be103db","last_name":"Schuster","first_name":"Maximilian"},{"full_name":"Kurtović, Katarina","last_name":"Kurtović","first_name":"Katarina"},{"first_name":"Ulrich Z.","full_name":"Hammes, Ulrich Z.","last_name":"Hammes"},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"OA_type":"hybrid","ddc":["580"],"pmid":1,"main_file_link":[{"url":"https://doi.org/10.1111/jipb.70309","open_access":"1"}],"researchdata_availability":"no","abstract":[{"text":"Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage.","lang":"eng"}],"publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"article_number":"jipb.70309","article_type":"original","das_tickbox":"0","acknowledgement":"Research in the Friml group was supported by the European Research Council (ERC) under grant agreement No. 101142681 (CYNIPS), and by the Austrian Science Fund (FWF) through projects I 6123-B and P 37051-B. A DOC Fellowship from the Austrian Academy of Sciences (ÖAW; PR.C0102.1.F.1023.A.2) provided additional support. Work was partly supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under grant HA 3468/8-1. We thank the Imaging and Optics Facility (IOF) at the Institute of Science and Technology Austria (ISTA) for support with confocal imaging, and the Nanofabrication Facility at ISTA for assistance with microfluidic device fabrication. We also acknowledge the microscopy service of IFIEB CAS, supported by MEYS CR (LM2023050 Czech-BioImaging). Open Access funding provided by Institute of Science and Technology Austria.","fulldoi":"https://doi.org/10.1111/jipb.70309","OA_place":"publisher","date_created":"2026-07-13T10:44:55Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"publication_status":"epub_ahead","acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"doi":"10.1111/jipb.70309","publisher":"Wiley","type":"journal_article","department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"NanoFab"},{"_id":"Bio"}],"year":"2026","has_accepted_license":"1","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"article_processing_charge":"Yes (via OA deal)","publication":"Journal of Integrative Plant Biology","citation":{"ama":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, et al. Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. 2026. doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>","ieee":"A. Smoljan <i>et al.</i>, “Auxin response and PIN‐mediated transport in chlorophyte algae,” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026.","apa":"Smoljan, A., Koutnik‐Abele, S., Vladimirtsev, D., Klíma, P., Bírošíková, A., Zhang, Y., … Friml, J. (2026). Auxin response and PIN‐mediated transport in chlorophyte algae. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>","chicago":"Smoljan, Adrijana, Sarah Koutnik‐Abele, Dmitrii Vladimirtsev, Petr Klíma, Anita Bírošíková, Yuzhou Zhang, Jack Merrin, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/jipb.70309\">https://doi.org/10.1111/jipb.70309</a>.","short":"A. Smoljan, S. Koutnik‐Abele, D. Vladimirtsev, P. Klíma, A. Bírošíková, Y. Zhang, J. Merrin, M. Schuster, K. Kurtović, U.Z. Hammes, J. Petrášek, J. Friml, Journal of Integrative Plant Biology (2026).","mla":"Smoljan, Adrijana, et al. “Auxin Response and PIN‐mediated Transport in Chlorophyte Algae.” <i>Journal of Integrative Plant Biology</i>, jipb. 70309, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/jipb.70309\">10.1111/jipb.70309</a>.","ista":"Smoljan A, Koutnik‐Abele S, Vladimirtsev D, Klíma P, Bírošíková A, Zhang Y, Merrin J, Schuster M, Kurtović K, Hammes UZ, Petrášek J, Friml J. 2026. Auxin response and PIN‐mediated transport in chlorophyte algae. Journal of Integrative Plant Biology., jipb. 70309."},"external_id":{"pmid":["42271607"]},"PlanS_conform":"1","day":"10","oa_version":"Published Version"},{"supplementarymaterial":"no","author":[{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"OA_type":"hybrid","researchdata_availability":"no","ddc":["580"],"pmid":1,"publication_identifier":{"eissn":["1878-4372"],"issn":["1360-1385"]},"article_type":"review","abstract":[{"lang":"eng","text":"The canonical mechanism by which the phytohormone auxin regulates transcription has been one of the cornerstones of plant signaling. The recent unexpected discovery of cyclic AMP (cAMP) as a second messenger in this pathway has revised its foundations while leaving many open questions and gaps in our understanding; these will be discussed in this forum article."}],"fulldoi":"https://doi.org/10.1016/j.tplants.2025.10.018","file":[{"date_updated":"2026-07-27T08:26:40Z","success":1,"file_size":432792,"checksum":"e60e903fb4b3e917dba763976ec652cd","file_id":"22413","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_name":"2026_TrendsPlantScience_Friml.pdf","relation":"main_file","date_created":"2026-07-27T08:26:40Z"}],"das_tickbox":"0","acknowledgement":"I apologize to colleagues whose relevant work I was unable to cite due to space limitations. This work was funded by the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; 37051-B). I thank Drs Huihuang Chen, Yuanrong Pei, Jason Reed, Linlin Qi, and Dolf Weijers for inspiration and critical input.","OA_place":"publisher","date_created":"2025-12-02T16:29:22Z","corr_author":"1","quality_controlled":"1","date_updated":"2026-07-27T08:27:07Z","scopus_import":"1","date_published":"2026-02-01T00:00:00Z","_id":"20725","project":[{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"month":"02","volume":31,"status":"public","title":"Role of cAMP in TIR1/AFB auxin signaling: Open issues","oa":1,"page":"136-138","file_date_updated":"2026-07-27T08:26:40Z","external_id":{"pmid":["41249070"]},"day":"01","oa_version":"Published Version","PlanS_conform":"1","intvolume":"        31","language":[{"iso":"eng"}],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Elsevier","doi":"10.1016/j.tplants.2025.10.018","type":"journal_article","year":"2026","issue":"2","department":[{"_id":"JiFr"}],"has_accepted_license":"1","publication":"Trends in Plant Science","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"article_processing_charge":"Yes (via OA deal)","citation":{"ama":"Friml J. Role of cAMP in TIR1/AFB auxin signaling: Open issues. <i>Trends in Plant Science</i>. 2026;31(2):136-138. doi:<a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">10.1016/j.tplants.2025.10.018</a>","ieee":"J. Friml, “Role of cAMP in TIR1/AFB auxin signaling: Open issues,” <i>Trends in Plant Science</i>, vol. 31, no. 2. Elsevier, pp. 136–138, 2026.","apa":"Friml, J. (2026). Role of cAMP in TIR1/AFB auxin signaling: Open issues. <i>Trends in Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">https://doi.org/10.1016/j.tplants.2025.10.018</a>","chicago":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” <i>Trends in Plant Science</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">https://doi.org/10.1016/j.tplants.2025.10.018</a>.","short":"J. Friml, Trends in Plant Science 31 (2026) 136–138.","mla":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” <i>Trends in Plant Science</i>, vol. 31, no. 2, Elsevier, 2026, pp. 136–38, doi:<a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">10.1016/j.tplants.2025.10.018</a>.","ista":"Friml J. 2026. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 31(2), 136–138."}},{"_id":"22315","date_published":"2026-07-09T00:00:00Z","scopus_import":"1","date_updated":"2026-08-04T09:22:49Z","quality_controlled":"1","dataavailabilitystatement":"All data are available in the manuscript or the supplementary materials. The raw RNA-seq data have been deposited in the NCBI Gene Expression Omnibus (GEO) under accession number GSE315473. Microbiome sequencing data have been deposited in the Sequence Read Archive (SRA) under BioProject number PRJNA1397137. Materials are available upon request from the corresponding author.","title":"Roots navigate around decay regions by sensing local pH gradients","status":"public","volume":393,"month":"07","project":[{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"pmid":1,"researchdata_availability":"yes","author":[{"first_name":"Zhulatai","full_name":"Bao, Zhulatai","last_name":"Bao"},{"last_name":"Wang","full_name":"Wang, Huihui","first_name":"Huihui"},{"full_name":"Zhang, Ai","last_name":"Zhang","first_name":"Ai"},{"full_name":"Gao, Ruxi","last_name":"Gao","first_name":"Ruxi"},{"last_name":"Gu","full_name":"Gu, Wen","first_name":"Wen"},{"full_name":"Fan, Ni","last_name":"Fan","first_name":"Ni"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Zhang, Yuzhou","last_name":"Zhang","first_name":"Yuzhou"}],"OA_type":"closed access","supplementarymaterial":"yes","date_created":"2026-07-13T14:57:10Z","das_tickbox":"1","acknowledgement":"We are grateful to H. Guo and L. Liu (Department of Biology, Southern University of Science and Technology) for providing the rgf1/2/3, rgi1/2/3/4, tpst-1, and pepr1/2 lines. We thank K.-h. Liu (College of Life Science, Northwest A&F University) for generously providing the ABA biosensor nlsABACUS2-400n. We also thank J. Li and J. Chang (School of Life Sciences, Lanzhou University) for providing the ahk2-5/cre1-2, ahp1/2/3, arr16/arr17, and pTCSn::GFP lines. Our thanks further extend to D. Qian, also from the School of Life Sciences at Lanzhou University, for sharing Arabidopsis line pTUB6::mCherry-TUB6. We are grateful to Y. Zhao (CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences) for providing nced3/5, snrk2.2/2.3/2.6, and pyl duodecuple mutants. We also acknowledge the Teaching and Research Core Facility at the College of Life Sciences, Northwest A&F University, particularly N. Fan, for their invaluable technical assistance. We also thank Life Science Research Core Services (LSRCS), Northwest A&F University, for helping with characterization, including CLSM (X. Liu). Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China JYB2025XDXM706 (Y.Z.); Qin Chuangyuan High-level Innovation and Entrepreneurship Talent Program QCYRCXM-2022-237 (Y.Z.); Fundamental Research Funds for the Central Universities K20200168 (Y.Z.); National Natural Science Foundation of China 32570375 (Y.Z.); National Natural Science Foundation of China 32400699 (A.Z.); European Research Council (ERC, CYNIPS) 101142681 (J.F.); Austrian Science Fund (FWF): P 37051-B (J.F.).","fulldoi":"https://doi.org/10.1126/science.adw6568","related_material":{"link":[{"url":"https://ista.ac.at/en/news/roots-steer-clear-of-plant-rot/","description":"News on ISTA website","relation":"press_release"}]},"abstract":[{"lang":"eng","text":"Plant tropisms enable roots to navigate complex soils by responding to directional environmental cues. Biological decay, although central to nutrient cycling, also creates microbially active and potentially hostile niches. In this work, we identified “saprotropism,” a previously unrecognized growth response that enables roots to actively bend away from decaying plant-derived matter. Fungal-driven microbial decomposition released organic acids and formed stable pH gradients in surrounding soil, allowing roots to pinpoint decay without direct contact. Root epidermal cells sensed this acidic gradient through the root meristem growth factor peptide-receptor module, converting external pH asymmetry into asymmetric abscisic acid (ABA) distribution. ABA asymmetry drove microtubule reorganization, which was decoded into decay-avoidant root bending. Together, these findings establish microbial decay–derived chemical gradients as an instructive signal for root navigation and expand the framework of microbe-soil-plant communication."}],"article_number":"eadw6568","article_type":"original","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"year":"2026","department":[{"_id":"JiFr"}],"issue":"6807","type":"journal_article","doi":"10.1126/science.adw6568","publisher":"American Association for the Advancement of Science","language":[{"iso":"eng"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_status":"published","citation":{"ista":"Bao Z, Wang H, Zhang A, Gao R, Gu W, Fan N, Friml J, Zhang Y. 2026. Roots navigate around decay regions by sensing local pH gradients. Science. 393(6807), eadw6568.","mla":"Bao, Zhulatai, et al. “Roots Navigate around Decay Regions by Sensing Local PH Gradients.” <i>Science</i>, vol. 393, no. 6807, eadw6568, American Association for the Advancement of Science, 2026, doi:<a href=\"https://doi.org/10.1126/science.adw6568\">10.1126/science.adw6568</a>.","ama":"Bao Z, Wang H, Zhang A, et al. Roots navigate around decay regions by sensing local pH gradients. <i>Science</i>. 2026;393(6807). doi:<a href=\"https://doi.org/10.1126/science.adw6568\">10.1126/science.adw6568</a>","ieee":"Z. Bao <i>et al.</i>, “Roots navigate around decay regions by sensing local pH gradients,” <i>Science</i>, vol. 393, no. 6807. American Association for the Advancement of Science, 2026.","short":"Z. Bao, H. Wang, A. Zhang, R. Gao, W. Gu, N. Fan, J. Friml, Y. Zhang, Science 393 (2026).","chicago":"Bao, Zhulatai, Huihui Wang, Ai Zhang, Ruxi Gao, Wen Gu, Ni Fan, Jiří Friml, and Yuzhou Zhang. “Roots Navigate around Decay Regions by Sensing Local PH Gradients.” <i>Science</i>. American Association for the Advancement of Science, 2026. <a href=\"https://doi.org/10.1126/science.adw6568\">https://doi.org/10.1126/science.adw6568</a>.","apa":"Bao, Z., Wang, H., Zhang, A., Gao, R., Gu, W., Fan, N., … Zhang, Y. (2026). Roots navigate around decay regions by sensing local pH gradients. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.adw6568\">https://doi.org/10.1126/science.adw6568</a>"},"article_processing_charge":"No","publication":"Science","external_id":{"pmid":["42424472"]},"intvolume":"       393","day":"09","oa_version":"None"},{"type":"journal_article","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"year":"2025","issue":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","language":[{"iso":"eng"}],"doi":"10.1038/s44383-025-00002-8","publisher":"Springer Nature","citation":{"ista":"Monzer A, Friml J. 2025. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. npj Science of Plants. 1(1), 2.","short":"A. Monzer, J. Friml, Npj Science of Plants 1 (2025) 2.","chicago":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” <i>Npj Science of Plants</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s44383-025-00002-8\">https://doi.org/10.1038/s44383-025-00002-8</a>.","apa":"Monzer, A., &#38; Friml, J. (2025). Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. <i>Npj Science of Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s44383-025-00002-8\">https://doi.org/10.1038/s44383-025-00002-8</a>","ieee":"A. Monzer and J. Friml, “Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.,” <i>npj Science of Plants</i>, vol. 1, no. 1. Springer Nature, p. 2, 2025.","ama":"Monzer A, Friml J. Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. <i>npj Science of Plants</i>. 2025;1(1):2. doi:<a href=\"https://doi.org/10.1038/s44383-025-00002-8\">10.1038/s44383-025-00002-8</a>","mla":"Monzer, Aline, and Jiří Friml. “Historical and Mechanistic Perspective on ABP1-TMK1-Mediated Cell Surface Auxin Signaling.” <i>Npj Science of Plants</i>, vol. 1, no. 1, Springer Nature, 2025, p. 2, doi:<a href=\"https://doi.org/10.1038/s44383-025-00002-8\">10.1038/s44383-025-00002-8</a>."},"has_accepted_license":"1","article_processing_charge":"Yes (in subscription journal)","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"publication":"npj Science of Plants","external_id":{"pmid":["40630787"]},"intvolume":"         1","day":"01","oa_version":"Published Version","date_published":"2025-07-01T00:00:00Z","_id":"21136","date_updated":"2026-02-10T09:39:20Z","corr_author":"1","quality_controlled":"1","title":"Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling.","status":"public","volume":1,"file_date_updated":"2026-02-10T09:35:43Z","page":"2","oa":1,"month":"07","project":[{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"},{"_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants"}],"ddc":["580"],"pmid":1,"OA_type":"hybrid","author":[{"first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","last_name":"Monzer","full_name":"Monzer, Aline"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2026-02-03T13:03:53Z","OA_place":"publisher","abstract":[{"text":"The plant hormone auxin regulates growth and development through at least two distinct signaling pathways. The nuclear pathway, involving TIR1/AFB receptors, mediates transcription; whereas the cell surface ABP1-TMK1 auxin perception triggers global ultrafast phosphorylation response. Here, we revisit the rich history of the disputed ABP1 auxin receptor, highlighting recent findings of the involvement of TMKs and other molecular components and focusing on their role in auxin canalization-mediated development.","lang":"eng"}],"publication_identifier":{"eissn":["3005-1401"]},"article_type":"original","acknowledgement":"We gratefully acknowledge the funding by the Austrian Science Fund (FWF; I 6123-B and P 37051-B) and the European Research Council (ERC; 101142681 CYNIPS).We would like to thank Lukas Fiedler for his significant input and thoughtful revision of this manuscript.","file":[{"success":1,"file_size":974106,"checksum":"6c190faacf0e3bef98311dc8a12132d4","date_updated":"2026-02-10T09:35:43Z","file_name":"2025_NPJSciencePlants_Monzer.pdf","relation":"main_file","date_created":"2026-02-10T09:35:43Z","access_level":"open_access","file_id":"21208","content_type":"application/pdf","creator":"dernst"}],"fulldoi":"https://doi.org/10.1038/s44383-025-00002-8"},{"citation":{"ieee":"H. Chen <i>et al.</i>, “TIR1-produced cAMP as a second messenger in transcriptional auxin signalling,” <i>Nature</i>, vol. 640. Springer Nature, pp. 1011–1016, 2025.","ama":"Chen H, Qi L, Zou M, et al. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. <i>Nature</i>. 2025;640:1011-1016. doi:<a href=\"https://doi.org/10.1038/s41586-025-08669-w\">10.1038/s41586-025-08669-w</a>","short":"H. Chen, L. Qi, M. Zou, M. Lu, M. Kwiatkowski, Y. Pei, K. Jaworski, J. Friml, Nature 640 (2025) 1011–1016.","chicago":"Chen, Huihuang, Linlin Qi, Minxia Zou, Mengting Lu, M Kwiatkowski, Yuanrong Pei, K Jaworski, and Jiří Friml. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” <i>Nature</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41586-025-08669-w\">https://doi.org/10.1038/s41586-025-08669-w</a>.","apa":"Chen, H., Qi, L., Zou, M., Lu, M., Kwiatkowski, M., Pei, Y., … Friml, J. (2025). TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-025-08669-w\">https://doi.org/10.1038/s41586-025-08669-w</a>","mla":"Chen, Huihuang, et al. “TIR1-Produced CAMP as a Second Messenger in Transcriptional Auxin Signalling.” <i>Nature</i>, vol. 640, Springer Nature, 2025, pp. 1011–16, doi:<a href=\"https://doi.org/10.1038/s41586-025-08669-w\">10.1038/s41586-025-08669-w</a>.","ista":"Chen H, Qi L, Zou M, Lu M, Kwiatkowski M, Pei Y, Jaworski K, Friml J. 2025. TIR1-produced cAMP as a second messenger in transcriptional auxin signalling. Nature. 640, 1011–1016."},"publication":"Nature","article_processing_charge":"Yes (via OA deal)","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","year":"2025","department":[{"_id":"JiFr"}],"isi":1,"type":"journal_article","publisher":"Springer Nature","doi":"10.1038/s41586-025-08669-w","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"publication_status":"published","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","intvolume":"       640","oa_version":"Published Version","day":"24","PlanS_conform":"1","external_id":{"isi":["001437493900001"],"pmid":["40044868"]},"oa":1,"file_date_updated":"2025-08-05T12:29:35Z","page":"1011-1016","volume":640,"status":"public","title":"TIR1-produced cAMP as a second messenger in transcriptional auxin signalling","project":[{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"month":"04","_id":"19421","date_published":"2025-04-24T00:00:00Z","quality_controlled":"1","corr_author":"1","date_updated":"2026-04-28T13:42:45Z","date_created":"2025-03-19T09:44:39Z","OA_place":"publisher","fulldoi":"https://doi.org/10.1038/s41586-025-08669-w","file":[{"content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_id":"20132","date_created":"2025-08-05T12:29:35Z","relation":"main_file","file_name":"2025_Nature_Chen.pdf","date_updated":"2025-08-05T12:29:35Z","file_size":13549245,"checksum":"f5f18081003e7a1b8e372ecb7da82e7d","success":1}],"related_material":{"record":[{"id":"19478","status":"public","relation":"dissertation_contains"}],"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/updating-the-textbook/","description":"News on ISTA website"}]},"acknowledgement":"We are grateful to J. Callis and H.-Q. Yang for sharing materials and to M. Estelle and S. Kepinski for inspiring discussions. This research was supported by the Laboratory Support Facility, the Plant Facility and the Imaging and Optics Facility of the Institute of Science and Technology Austria. This project has received funding from the European Research Council (101142681 CYNIPS) and Austrian Science Fund (P 37051-B). L.Q. was supported by the National Natural Science Foundation of China (grant no. 32470327). M.Z. was supported by the Interdisciplinary Project Committee of the Institute of Science and Technology Austria, and Y.P. was supported by an EMBO Postdoctoral Fellowship (ALTF 38-2023). Open access funding provided by Institute of Science and Technology (IST Austria).","article_type":"original","publication_identifier":{"eissn":["1476-4687"],"issn":["0028-0836"]},"abstract":[{"lang":"eng","text":"The phytohormone auxin (Aux) is a principal endogenous developmental signal in plants. It mediates transcriptional reprogramming by a well-established canonical signalling mechanism. TIR1/AFB auxin receptors are F-box subunits of an ubiquitin ligase complex; after auxin perception, they associate with Aux/IAA transcriptional repressors and ubiquitinate them for degradation, thus enabling the activation of auxin response factor (ARF) transcription factors1,2,3. Here we revise this paradigm by showing that without TIR1 adenylate cyclase (AC) activity4, auxin-induced degradation of Aux/IAAs is not sufficient to mediate the transcriptional auxin response. Abolishing the TIR1 AC activity does not affect auxin-induced degradation of Aux/IAAs but renders TIR1 non-functional in mediating transcriptional reprogramming and auxin-regulated development, including shoot, root, root hair growth and lateral root formation. Transgenic plants show that local cAMP production in the vicinity of the Aux/IAA–ARF complex by unrelated AC enzymes bypasses the need for auxin perception and is sufficient to induce ARF-mediated transcription. These discoveries revise the canonical model of auxin signalling and establish TIR1/AFB-produced cAMP as a second messenger essential for transcriptional reprograming."}],"ddc":["580"],"pmid":1,"author":[{"first_name":"Huihuang","id":"83c96512-15b2-11ec-abd3-b7eede36184f","last_name":"Chen","full_name":"Chen, Huihuang"},{"full_name":"Qi, Linlin","last_name":"Qi","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","orcid":"0000-0001-5187-8401","first_name":"Linlin"},{"last_name":"Zou","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","first_name":"Minxia"},{"first_name":"Mengting","last_name":"Lu","id":"a8198a14-1ffe-11ee-8b67-d2bdff9d9178","full_name":"Lu, Mengting"},{"last_name":"Kwiatkowski","full_name":"Kwiatkowski, M","first_name":"M"},{"first_name":"Yuanrong","last_name":"Pei","id":"98605edc-6ce7-11ee-95f3-cc16b866efcd","full_name":"Pei, Yuanrong"},{"full_name":"Jaworski, K","last_name":"Jaworski","first_name":"K"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"OA_type":"hybrid"},{"OA_place":"publisher","date_created":"2025-04-04T07:48:24Z","file":[{"date_updated":"2025-04-08T08:22:37Z","file_size":16344814,"checksum":"b154973663a1bba505683faab7ae5ead","file_id":"19526","access_level":"closed","creator":"hchen","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_name":"Thesis_0403_Huihuang.docx","relation":"source_file","date_created":"2025-04-08T08:00:07Z"},{"file_size":8482147,"checksum":"0099565f024388830c125ec17375c1a0","embargo_to":"local","date_updated":"2025-04-09T13:53:38Z","relation":"main_file","file_name":"Thesis_0406_PDFA_Huihuang_1.pdf","embargo":"2026-10-08","date_created":"2025-04-08T08:00:06Z","content_type":"application/pdf","creator":"hchen","file_id":"19527","access_level":"closed"}],"fulldoi":"https://doi.org/10.15479/AT-ISTA-19478","related_material":{"record":[{"relation":"part_of_dissertation","id":"19421","status":"public"},{"relation":"part_of_dissertation","status":"public","id":"13212"}]},"acknowledgement":"This project was funded by the European Research Council Advanced Grant (ETAP-742985),\r\nEuropean Research Council (ERC; 101142681 CYNIPS), Austrian Science Fund (FWF; P\r\n37051-B).","publication_identifier":{"issn":["2663-337X"]},"ddc":["580"],"author":[{"full_name":"Chen, Huihuang","last_name":"Chen","id":"83c96512-15b2-11ec-abd3-b7eede36184f","first_name":"Huihuang"}],"degree_awarded":"PhD","page":"118","file_date_updated":"2025-04-09T13:53:38Z","status":"public","title":"The cAMP second messenger in auxin signalling","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"},{"name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","grant_number":"101142681"}],"month":"04","_id":"19478","date_published":"2025-04-04T00:00:00Z","corr_author":"1","date_updated":"2026-07-06T12:58:58Z","supervisor":[{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"day":"04","oa_version":"Published Version","citation":{"ista":"Chen H. 2025. The cAMP second messenger in auxin signalling. Institute of Science and Technology Austria.","mla":"Chen, Huihuang. <i>The CAMP Second Messenger in Auxin Signalling</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19478\">10.15479/AT-ISTA-19478</a>.","ama":"Chen H. The cAMP second messenger in auxin signalling. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19478\">10.15479/AT-ISTA-19478</a>","ieee":"H. Chen, “The cAMP second messenger in auxin signalling,” Institute of Science and Technology Austria, 2025.","short":"H. Chen, The CAMP Second Messenger in Auxin Signalling, Institute of Science and Technology Austria, 2025.","chicago":"Chen, Huihuang. “The CAMP Second Messenger in Auxin Signalling.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19478\">https://doi.org/10.15479/AT-ISTA-19478</a>.","apa":"Chen, H. (2025). <i>The cAMP second messenger in auxin signalling</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19478\">https://doi.org/10.15479/AT-ISTA-19478</a>"},"article_processing_charge":"No","has_accepted_license":"1","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"year":"2025","type":"dissertation","publisher":"Institute of Science and Technology Austria","ec_funded":1,"doi":"10.15479/AT-ISTA-19478","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"alternative_title":["ISTA Thesis"],"language":[{"iso":"eng"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","publication_status":"published"},{"citation":{"ama":"Luschnig C, Friml J. Over 25 years of decrypting PIN-mediated plant development. <i>Nature Communications</i>. 2024;15. doi:<a href=\"https://doi.org/10.1038/s41467-024-54240-y\">10.1038/s41467-024-54240-y</a>","ieee":"C. Luschnig and J. Friml, “Over 25 years of decrypting PIN-mediated plant development,” <i>Nature Communications</i>, vol. 15. Springer Nature, 2024.","apa":"Luschnig, C., &#38; Friml, J. (2024). Over 25 years of decrypting PIN-mediated plant development. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-024-54240-y\">https://doi.org/10.1038/s41467-024-54240-y</a>","short":"C. Luschnig, J. Friml, Nature Communications 15 (2024).","chicago":"Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated Plant Development.” <i>Nature Communications</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41467-024-54240-y\">https://doi.org/10.1038/s41467-024-54240-y</a>.","mla":"Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated Plant Development.” <i>Nature Communications</i>, vol. 15, 9904, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s41467-024-54240-y\">10.1038/s41467-024-54240-y</a>.","ista":"Luschnig C, Friml J. 2024. Over 25 years of decrypting PIN-mediated plant development. Nature Communications. 15, 9904."},"has_accepted_license":"1","publication":"Nature Communications","article_processing_charge":"Yes","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"isi":1,"type":"journal_article","department":[{"_id":"JiFr"}],"year":"2024","publication_status":"published","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"publisher":"Springer Nature","doi":"10.1038/s41467-024-54240-y","intvolume":"        15","day":"01","oa_version":"Published Version","external_id":{"isi":["001356232600004"],"pmid":["39548100"]},"status":"public","volume":15,"title":"Over 25 years of decrypting PIN-mediated plant development","oa":1,"file_date_updated":"2024-12-03T14:10:54Z","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"},{"_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors"}],"month":"12","scopus_import":"1","date_published":"2024-12-01T00:00:00Z","_id":"18582","quality_controlled":"1","corr_author":"1","DOAJ_listed":"1","date_updated":"2025-09-08T14:53:48Z","OA_place":"publisher","date_created":"2024-11-24T23:01:48Z","article_type":"original","article_number":"9904","publication_identifier":{"eissn":["2041-1723"]},"abstract":[{"text":"Identification of PIN exporters for auxin, the major coordinative signal in plants, some 25 years ago, signifies a landmark in our understanding of plant-specific mechanisms underlying development and adaptation. Auxin is directionally transported throughout the plant body; a unique feature already envisioned by Darwin and solidified by PINs’ discovery and characterization. The PIN-based auxin distribution network with its complex regulations of PIN expression, localization and activity turned out to underlie a remarkable multitude of developmental processes and represents means to integrate endogenous and environmental signals. Given the recent anniversary, we here summarize past and current developments in this exciting field.","lang":"eng"}],"file":[{"date_updated":"2024-12-03T14:10:54Z","success":1,"file_size":1426555,"checksum":"3a31af06f52100d287f1e9d9c2aa1d40","access_level":"open_access","file_id":"18615","creator":"dernst","content_type":"application/pdf","date_created":"2024-12-03T14:10:54Z","file_name":"2024_NatureComm_Luschnig.pdf","relation":"main_file"}],"fulldoi":"https://doi.org/10.1038/s41467-024-54240-y","acknowledgement":"We gratefully acknowledge Leo Gälweiler for authorizing his PIN1 story. We would like to thank Yuanrong Pei for invaluable help with preparing figures. Work in the lab of C.L. is supported by grants from the Austrian Science Fund (PAT 8419423) and by the Gesellschaft für Forschungsförderung Niederösterreich m.b.H. (FTI19-008). The lab of J.F. is supported by the Austrian Science Fund (I 6123-B and P 37051-B).","pmid":1,"ddc":["580"],"OA_type":"gold","author":[{"first_name":"Christian","last_name":"Luschnig","full_name":"Luschnig, Christian"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}]},{"project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"month":"09","status":"public","volume":7,"title":"Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis","oa":1,"file_date_updated":"2024-09-17T09:44:29Z","quality_controlled":"1","date_updated":"2026-09-11T22:30:08Z","scopus_import":"1","date_published":"2024-09-04T00:00:00Z","_id":"18063","article_type":"original","article_number":"1085","publication_identifier":{"eissn":["2399-3642"]},"abstract":[{"text":"The developmental plasticity of the root system plays an essential role in the adaptation of plants to the environment. Among many other signals, auxin and its directional, intercellular transport are critical in regulating root growth and development. In particular, the PIN-FORMED2 (PIN2) auxin exporter acts as a key regulator of root gravitropic growth. Multiple regulators have been reported to be involved in PIN2-mediated root growth; however, our information remains incomplete. Here, we identified ROWY Bro1-domain proteins as important regulators of PIN2 sorting control. Genetic analysis revealed that Arabidopsis rowy1 single mutants and higher-order rowy1 rowy2 rowy3 triple mutants presented a wavy root growth phenotype. Cell biological experiments revealed that ROWY1 and PIN2 colocalized to the apical side of the plasma membrane in the root epidermis and that ROWYs are required for correct PM targeting of PIN2. In addition, ROWYs also affected PIN3 protein abundance in the stele, suggesting the potential involvement of additional PIN transporters as well as other proteins. A global transcriptome analysis revealed that ROWY genes are involved in the Fe2+ availability perception pathway. This work establishes ROWYs as important novel regulators of root gravitropic growth by connecting micronutrient availability to the proper subcellular targeting of PIN auxin transporters.","lang":"eng"}],"fulldoi":"https://doi.org/10.1038/s42003-024-06747-9","related_material":{"record":[{"id":"20117","status":"public","relation":"dissertation_contains"}]},"file":[{"date_created":"2024-09-17T09:44:29Z","file_name":"2024_CommBiology_Peng.pdf","relation":"main_file","file_id":"18084","access_level":"open_access","content_type":"application/pdf","creator":"dernst","success":1,"file_size":7718758,"checksum":"7d66af41c90e73d1b8a375eb652a9561","date_updated":"2024-09-17T09:44:29Z"}],"acknowledgement":"We thank Drs. Erika Isono (University of Constance), Grégory Vert (University of Toulouse), and Liwen Jiang (The Chinese University of Hong Kong) for kindly sharing published Arabidopsis lines; Dr. Yuzhou Zhang (ISTA) for help with molecular cloning, and Drs. Melinda Abas (BOKU), Eugenia Russinova (Ghent University), and Zhaojun Ding (Shandong University) for valuable discussions. This work was supported by grants to S.T. from the National Natural Science Foundation of China (32321001), the USTC Research Funds of the Double First-Class Initiative (YD9100002016), the Research Funds from the Center for Advanced Interdisciplinary Science and Biomedicine of IHM, the Division of Life Sciences and Medicine, the University of Science and Technology of China (QYPY20220012), the Fundamental Research Funds for the Central Universities (WK9100000021), and start-up funding from the University of Science and Technology of China and the Chinese Academy of Sciences (GG9100007007, KY9100000026, KY9100000051, and KJ2070000079). J.S. was supported by the National Natural Science Foundation of China (31970181 and 32170342). J.F. was supported by Austrian Science Fund (FWF; projects I6123 and P37051-B).","date_created":"2024-09-15T22:01:38Z","author":[{"full_name":"Peng, Yakun","last_name":"Peng","first_name":"Yakun"},{"last_name":"Ji","full_name":"Ji, Kangkang","first_name":"Kangkang"},{"full_name":"Mao, Yanbo","last_name":"Mao","first_name":"Yanbo"},{"first_name":"Yiqun","full_name":"Wang, Yiqun","last_name":"Wang","id":"82F537F2-B517-11E9-84D7-6433E6697425"},{"last_name":"Korbei","full_name":"Korbei, Barbara","first_name":"Barbara"},{"full_name":"Luschnig, Christian","last_name":"Luschnig","first_name":"Christian"},{"full_name":"Shen, Jinbo","last_name":"Shen","first_name":"Jinbo"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"first_name":"Shutang","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan","full_name":"Tan, Shutang"}],"ddc":["570"],"pmid":1,"has_accepted_license":"1","publication":"Communications Biology","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"article_processing_charge":"Yes","citation":{"mla":"Peng, Yakun, et al. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth in Arabidopsis.” <i>Communications Biology</i>, vol. 7, 1085, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s42003-024-06747-9\">10.1038/s42003-024-06747-9</a>.","short":"Y. Peng, K. Ji, Y. Mao, Y. Wang, B. Korbei, C. Luschnig, J. Shen, E. Benková, J. Friml, S. Tan, Communications Biology 7 (2024).","chicago":"Peng, Yakun, Kangkang Ji, Yanbo Mao, Yiqun Wang, Barbara Korbei, Christian Luschnig, Jinbo Shen, Eva Benková, Jiří Friml, and Shutang Tan. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth in Arabidopsis.” <i>Communications Biology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s42003-024-06747-9\">https://doi.org/10.1038/s42003-024-06747-9</a>.","apa":"Peng, Y., Ji, K., Mao, Y., Wang, Y., Korbei, B., Luschnig, C., … Tan, S. (2024). Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-024-06747-9\">https://doi.org/10.1038/s42003-024-06747-9</a>","ieee":"Y. Peng <i>et al.</i>, “Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis,” <i>Communications Biology</i>, vol. 7. Springer Nature, 2024.","ama":"Peng Y, Ji K, Mao Y, et al. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. <i>Communications Biology</i>. 2024;7. doi:<a href=\"https://doi.org/10.1038/s42003-024-06747-9\">10.1038/s42003-024-06747-9</a>","ista":"Peng Y, Ji K, Mao Y, Wang Y, Korbei B, Luschnig C, Shen J, Benková E, Friml J, Tan S. 2024. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. Communications Biology. 7, 1085."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"publication_status":"published","publisher":"Springer Nature","doi":"10.1038/s42003-024-06747-9","isi":1,"type":"journal_article","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"year":"2024","day":"04","oa_version":"Published Version","intvolume":"         7","external_id":{"isi":["001306499600002"],"pmid":["39232040"]}},{"issue":"2","year":"2023","department":[{"_id":"JiFr"}],"type":"journal_article","isi":1,"doi":"10.1111/nph.19123","publisher":"Wiley","language":[{"iso":"eng"}],"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling and beyond. New Phytologist. 240(2), 489–495.","short":"L. Qi, J. Friml, New Phytologist 240 (2023) 489–495.","chicago":"Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling and Beyond.” <i>New Phytologist</i>. Wiley, 2023. <a href=\"https://doi.org/10.1111/nph.19123\">https://doi.org/10.1111/nph.19123</a>.","apa":"Qi, L., &#38; Friml, J. (2023). Tale of cAMP as a second messenger in auxin signaling and beyond. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.19123\">https://doi.org/10.1111/nph.19123</a>","ama":"Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond. <i>New Phytologist</i>. 2023;240(2):489-495. doi:<a href=\"https://doi.org/10.1111/nph.19123\">10.1111/nph.19123</a>","ieee":"L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling and beyond,” <i>New Phytologist</i>, vol. 240, no. 2. Wiley, pp. 489–495, 2023.","mla":"Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling and Beyond.” <i>New Phytologist</i>, vol. 240, no. 2, Wiley, 2023, pp. 489–95, doi:<a href=\"https://doi.org/10.1111/nph.19123\">10.1111/nph.19123</a>."},"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"article_processing_charge":"Yes (via OA deal)","publication":"New Phytologist","has_accepted_license":"1","external_id":{"pmid":["37434303"],"isi":["001026321500001"]},"intvolume":"       240","day":"01","oa_version":"Published Version","_id":"13266","date_published":"2023-10-01T00:00:00Z","scopus_import":"1","date_updated":"2024-10-22T12:50:00Z","corr_author":"1","quality_controlled":"1","file_date_updated":"2024-01-29T11:21:43Z","page":"489-495","oa":1,"title":"Tale of cAMP as a second messenger in auxin signaling and beyond","volume":240,"status":"public","month":"10","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"pmid":1,"ddc":["580"],"author":[{"id":"44B04502-A9ED-11E9-B6FC-583AE6697425","last_name":"Qi","full_name":"Qi, Linlin","first_name":"Linlin","orcid":"0000-0001-5187-8401"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"date_created":"2023-07-23T22:01:13Z","acknowledgement":"We gratefully acknowledge our brave colleagues, whose excellent efforts kept the plant cAMP research going in the last two decades. The authors were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.","file":[{"creator":"dernst","content_type":"application/pdf","access_level":"open_access","file_id":"14898","relation":"main_file","file_name":"2023_NewPhytologist_Qi.pdf","date_created":"2024-01-29T11:21:43Z","date_updated":"2024-01-29T11:21:43Z","file_size":974464,"checksum":"6d9bbd45b8e7bb3ceee2586d447bacb2","success":1}],"fulldoi":"https://doi.org/10.1111/nph.19123","abstract":[{"text":"The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger in many mammalian signaling pathways. However, its role in plants remains not well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors and the demonstration of its importance for canonical auxin signaling put plant cAMP research back into spotlight. This insight briefly summarizes the well-established cAMP signaling pathways in mammalian cells and describes the turbulent and controversial history of plant cAMP research highlighting the major progress and the unresolved points. We also briefly review the current paradigm of auxin signaling to provide a background for the discussion on the AC activity of TIR1/AFB auxin receptors and its potential role in transcriptional auxin signaling as well as impact of these discoveries on plant cAMP research in general.","lang":"eng"}],"article_type":"original","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]}}]