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- _id: EM-Fac
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- _id: LifeSc
acknowledgement: We thank all members of the Heisenberg, Henkes, and Hannezo groups
for their support. We are also grateful to the Imaging and Optics, Scientific Computing,
Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their
technical assistance and support. Numerical simulations were performed using the
computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary
Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided
by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020
research and innovation programme (grant agreement No. 665385). This work was supported
by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to
C.-P.H.
article_processing_charge: No
author:
- first_name: Suyash
full_name: Naik, Suyash
id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
last_name: Naik
orcid: 0000-0001-8421-5508
citation:
ama: Naik S. Data associated with Keratins coordinate tissue spreading . 2026. doi:10.15479/AT-ISTA-21137
apa: Naik, S. (2026). Data associated with Keratins coordinate tissue spreading
. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-21137
chicago: Naik, Suyash. “Data Associated with Keratins Coordinate Tissue Spreading
.” Institute of Science and Technology Austria, 2026. https://doi.org/10.15479/AT-ISTA-21137.
ieee: S. Naik, “Data associated with Keratins coordinate tissue spreading .” Institute
of Science and Technology Austria, 2026.
ista: Naik S. 2026. Data associated with Keratins coordinate tissue spreading ,
Institute of Science and Technology Austria, 10.15479/AT-ISTA-21137.
mla: Naik, Suyash. Data Associated with Keratins Coordinate Tissue Spreading
. Institute of Science and Technology Austria, 2026, doi:10.15479/AT-ISTA-21137.
short: S. Naik, (2026).
contributor:
- contributor_type: researcher
first_name: Yann-Edwin
last_name: Keta
- contributor_type: supervisor
first_name: 'Silke '
last_name: Henkes
- contributor_type: supervisor
first_name: Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- contributor_type: supervisor
first_name: Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
corr_author: '1'
date_created: 2026-02-04T16:38:02Z
date_published: 2026-03-24T00:00:00Z
date_updated: 2026-03-24T08:32:00Z
day: '24'
department:
- _id: GradSch
- _id: CaHe
- _id: EdHa
doi: 10.15479/AT-ISTA-21137
ec_funded: 1
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date_created: 2026-03-16T11:51:10Z
date_updated: 2026-03-16T11:51:10Z
description: 'Python3 library written in C++20 to integrate vertex models. Please
read the readme at https://github.com/yketa/cells/blob/main/README.md for detailed
instructions for installation and usage of the code in this repository. '
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call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 8f060199-16d5-11f0-9cad-f3253b266c46
grant_number: PAT 5044023
name: Keratins in epithelial tissue spreading
- _id: 252C3B08-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1250-B20
name: Nano-Analytics of Cellular Systems
publisher: Institute of Science and Technology Austria
status: public
title: 'Data associated with Keratins coordinate tissue spreading '
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image: /images/cc_by_sa.png
legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode
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BY-SA 4.0)
short: CC BY-SA (4.0)
type: research_data
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...
---
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_id: '20441'
abstract:
- lang: eng
text: "Epithelial spreading plays a pivotal role in the development of organisms
especially those\r\nsuch as zebrafish which require the epithelial enveloping
layer (EVL) to spread to cover the\r\nsubstantial yolk surface during gastrulation.
Epiboly requires the transition of the epithelium\r\nwith cuboidal cells to form
a thin, flat squamous epithelial sheet. During this transition, the\r\ncells show
tissue-scale mechanosensation with mechanisms such as direct mechanical control\r\nover
the axis of cell division.\r\nCytoskeletal intermediate filaments play a crucial
role in vertebrate cells, not only facilitating\r\nmechanical stability but also
helping facilitate the mechanosensitive response of the cell.\r\nMechanosenstivity
displayed by intermediate filaments is due not just to their interesting\r\nphysical
properties but also to their interactions with other cytoskeletal elements such
as actin\r\nand microtubules. Keratin is the predominant intermediate filament
expressed in the EVL.\r\nIt expresses concomitantly with the gastrulation movements
of the developing embryo. Our\r\nwork focuses on understanding the role and dynamics
of the keratin cytoskeletal network in\r\nmodulating the physical aspects of EVL
spreading. We demonstrated with the combination of\r\nphysical characterisation
and manipulations of the EVL, utilising a variety of biophysical tools\r\nand
microscopy, the mechanistic role of keratin in tissue spreading.\r\nGenerating
novel genetic morphants and mutants, we probe the effect that the loss of the\r\nkeratin
network has on the physiology of the epithelium and the developing embryo. We\r\nshow
that the changing organisation of the keratin network is important for changing
EVL\r\nphysical properties as the stress imposed on the EVL increases during epiboly.
By modelling\r\nthe epithelium, we study how the mechanical heterogeneity in an
epithelium can feed back into\r\na mechanical loop to the maturation of the keratin
network and hence affect the mechanics\r\nof the epithelium. However, unlike what
would be predicted by the effect of intermediate\r\nfilaments in acting as a security
belt and increasing the resistance of the epithelium, we observe\r\nthat loss
of keratin leads to a delay in the EVL movement. Using both local aspirations
of the\r\nYSL and EVL ablations, we demonstrate the mechanistic facilitation of
actin mechanosensation\r\nin a keratin-dependent manner.\r\nFurthermore, using
chemical inhibitors of microtubule polymerisation, we provide insight into\r\nthe
mechanisms underlying the organisation and distribution of keratin. Interestingly,
the\r\nphenotype observed upon this loss of microtubules shows that keratins interact
with the nucleus\r\nthrough microtubular interactions. Together with these diverse
observations, we describe\r\nthe mechanosensory feedback between resilience and
that is critical for uniform and robust\r\nspreading of the epithelium."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "I would also like to thank the LSF and Cryo facility at ISTA, which
have been helpful in my\r\nexperiments. I would also like to acknowledge FWF, grant
DOI 10.55776/PAT5044023 and JKU Nanocell grant DOI \r\n10.55776/W1250 for providing
funding for my PhD research. EMBO and FWF for providing funding for travel grants
to attend conferences."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Suyash
full_name: Naik, Suyash
id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
last_name: Naik
orcid: 0000-0001-8421-5508
citation:
ama: Naik S. Keratins act as global coordinators of tissue spreading through mechanosensitive
feedback. 2025. doi:10.15479/AT-ISTA-20441
apa: Naik, S. (2025). Keratins act as global coordinators of tissue spreading
through mechanosensitive feedback. Institute of Science and Technology Austria.
https://doi.org/10.15479/AT-ISTA-20441
chicago: Naik, Suyash. “Keratins Act as Global Coordinators of Tissue Spreading
through Mechanosensitive Feedback.” Institute of Science and Technology Austria,
2025. https://doi.org/10.15479/AT-ISTA-20441.
ieee: S. Naik, “Keratins act as global coordinators of tissue spreading through
mechanosensitive feedback,” Institute of Science and Technology Austria, 2025.
ista: Naik S. 2025. Keratins act as global coordinators of tissue spreading through
mechanosensitive feedback. Institute of Science and Technology Austria.
mla: Naik, Suyash. Keratins Act as Global Coordinators of Tissue Spreading through
Mechanosensitive Feedback. Institute of Science and Technology Austria, 2025,
doi:10.15479/AT-ISTA-20441.
short: S. Naik, Keratins Act as Global Coordinators of Tissue Spreading through
Mechanosensitive Feedback, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-10-10T14:58:30Z
date_published: 2025-10-12T00:00:00Z
date_updated: 2026-04-07T11:58:57Z
day: '12'
ddc:
- '596'
- '597'
- '532'
degree_awarded: PhD
department:
- _id: GradSch
- _id: CaHe
- _id: EdHa
doi: 10.15479/AT-ISTA-20441
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month: '10'
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grant_number: PAT 5044023
name: Keratins in epithelial tissue spreading
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call_identifier: FWF
grant_number: W 1250-B20
name: Nano-Analytics of Cellular Systems
publication_identifier:
isbn:
- 978-3-99078-069-5
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
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relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
title: Keratins act as global coordinators of tissue spreading through mechanosensitive
feedback
tmp:
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name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC
BY-SA 4.0)
short: CC BY-SA (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...