[{"month":"04","quality_controlled":"1","file_date_updated":"2026-05-04T12:20:10Z","type":"journal_article","title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","status":"public","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"intvolume":"        45","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"has_accepted_license":"1","external_id":{"pmid":["41964955 "]},"OA_place":"publisher","_id":"21746","publication":"Cell Reports","related_material":{"record":[{"relation":"dissertation_contains","id":"22667","status":"public"}]},"day":"28","publisher":"Elsevier","publication_status":"published","OA_type":"gold","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2026-04-28T00:00:00Z","date_created":"2026-04-19T22:07:43Z","oa_version":"Published Version","fulldoi":"https://doi.org/10.1016/j.celrep.2026.117227","corr_author":"1","file":[{"success":1,"relation":"main_file","date_updated":"2026-05-04T12:20:10Z","creator":"dernst","date_created":"2026-05-04T12:20:10Z","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef","file_size":14925958,"access_level":"open_access","content_type":"application/pdf","file_id":"21795","file_name":"2026_CellReports_Vijatovic.pdf"}],"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"article_number":"117227","pmid":1,"PlanS_conform":"1","oa":1,"DOAJ_listed":"1","language":[{"iso":"eng"}],"author":[{"orcid":"0000-0002-5494-0941","full_name":"Vijatovic, David","first_name":"David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"full_name":"Toma, Florina Alexandra ","first_name":"Florina Alexandra ","last_name":"Toma","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e"},{"last_name":"Ignatyev","first_name":"Y","full_name":"Ignatyev, Y"},{"first_name":"Zoe P","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","last_name":"Harrington","full_name":"Harrington, Zoe P","orcid":"0009-0008-0158-4032"},{"first_name":"Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"full_name":"Smits, Matthijs Geert","first_name":"Matthijs Geert","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","last_name":"Smits"},{"first_name":"Marco","last_name":"Dalla Vecchia","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","full_name":"Dalla Vecchia, Marco"},{"first_name":"Alexandra J.","last_name":"Trevisan","full_name":"Trevisan, Alexandra J."},{"full_name":"Chapman, Phillip","first_name":"Phillip","last_name":"Chapman"},{"first_name":"Mara","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","last_name":"Julseth","full_name":"Julseth, Mara"},{"full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton","first_name":"Susan"},{"first_name":"Mariano I.","last_name":"Gabitto","full_name":"Gabitto, Mariano I."},{"full_name":"Dasen, Jeremy S.","first_name":"Jeremy S.","last_name":"Dasen"},{"full_name":"Bikoff, Jay B.","first_name":"Jay B.","last_name":"Bikoff"},{"full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","first_name":"Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","last_name":"Sweeney"}],"date_updated":"2026-09-16T07:33:54Z","citation":{"short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>.","chicago":"Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>.","apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>","ieee":"D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier, 2026."},"abstract":[{"lang":"eng","text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution."}],"year":"2026","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","scopus_import":"1","ddc":["570"],"doi":"10.1016/j.celrep.2026.117227","volume":45,"project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"},{"grant_number":"CZI01","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy"},{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"name":"Neuronal circuits in health and disease (Sweeney)","_id":"cf428362-b037-11f1-b015-8277a8a2f63d","grant_number":"COE16"}],"article_type":"original","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"issue":"4","article_processing_charge":"Yes"},{"doi":"10.1007/978-1-0716-5360-9_9","ddc":["570"],"abstract":[{"lang":"eng","text":"Adeno-associated viruses (AAVs) provide a versatile tool for labeling neurons in the Xenopus central nervous system across developmental stages. AAV-mediated transgene expression is long-lasting and stable, enabling robust labeling of neuronal populations without genomic integration and without the need to generate transgenic lines. The choice of AAV capsid and promoter can restrict expression to a population of interest, and depending on the route and timing of delivery, AAVs can selectively target either progenitor-derived neuronal cohorts or mature neuronal populations. Here, we have outlined two labeling strategies. First, intraventricular injections in tadpoles transduce neural progenitor cells lining the ventricular system, resulting in cohort-based labeling of neurons as they differentiate during development. Second, direct intraparenchymal injections in post-metamorphic frogs enable spatially restricted labeling and anterograde or retrograde tracing of connectivity within defined brain regions. Together, these approaches provide selective access to developing and mature neural circuits in X. laevis, supporting applications ranging from anatomical tracing to functional imaging and manipulation of neural circuits."}],"acknowledgement":"This work was supported by a Horizon Europe ERC Starting Grant Number 101041551 (L.B.S. and D.V.), FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.), NSF IOS Grant Number 2110086 (L.B.S. and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B (L.B.S.), and Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S. and D.V.).","year":"2026","scopus_import":"1","citation":{"short":"D. Vijatovic, L.B. Sweeney, in:, C.W. Beck (Ed.), Xenopus, Springer, 2026, pp. 219–229.","mla":"Vijatovic, David, and Lora B. Sweeney. “Adeno-Associated Virus (AAV) for Tracking Neural Development and Connectivity in Xenopus.” <i>Xenopus</i>, edited by Caroline W. Beck, vol. 3049, Springer, 2026, pp. 219–29, doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">10.1007/978-1-0716-5360-9_9</a>.","chicago":"Vijatovic, David, and Lora B. Sweeney. “Adeno-Associated Virus (AAV) for Tracking Neural Development and Connectivity in Xenopus.” In <i>Xenopus</i>, edited by Caroline W. Beck, 3049:219–29. MIMB. Springer, 2026. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">https://doi.org/10.1007/978-1-0716-5360-9_9</a>.","apa":"Vijatovic, D., &#38; Sweeney, L. B. (2026). Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus. In C. W. Beck (Ed.), <i>Xenopus</i> (Vol. 3049, pp. 219–229). Springer. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">https://doi.org/10.1007/978-1-0716-5360-9_9</a>","ama":"Vijatovic D, Sweeney LB. Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus. In: Beck CW, ed. <i>Xenopus</i>. Vol 3049. MIMB. Springer; 2026:219-229. doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_9\">10.1007/978-1-0716-5360-9_9</a>","ista":"Vijatovic D, Sweeney LB. 2026.Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus. In: Xenopus. Methods in Molecular Biology, vol. 3049, 219–229.","ieee":"D. Vijatovic and L. B. Sweeney, “Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus,” in <i>Xenopus</i>, vol. 3049, C. W. Beck, Ed. Springer, 2026, pp. 219–229."},"alternative_title":["Methods in Molecular Biology"],"main_file_link":[{"url":"https://doi.org/10.1007/978-1-0716-5360-9_9","open_access":"1"}],"article_processing_charge":"Yes (in subscription journal)","department":[{"_id":"LoSw"}],"project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"},{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity"},{"grant_number":"COE16","_id":"cf428362-b037-11f1-b015-8277a8a2f63d","name":"Neuronal circuits in health and disease (Sweeney)"},{"grant_number":"3(GG016346-01)","_id":"34a02c70-11ca-11ed-8bc3-fbfd2c86c88f","name":"Development of Viral Vectors for Amphibian Gene Delivery and Manipulation"}],"volume":3049,"publication_identifier":{"eissn":["1940-6029"]},"file":[{"checksum":"ea5af1374dd6820830773e0e96535ac0","file_size":852042,"date_created":"2026-09-17T08:00:45Z","date_updated":"2026-09-17T08:00:45Z","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_id":"22939","file_name":"2026_MIMB_Vijatovic2.pdf","success":1,"relation":"main_file"}],"editor":[{"last_name":"Beck","first_name":"Caroline W.","full_name":"Beck, Caroline W."}],"fulldoi":"https://doi.org/10.1007/978-1-0716-5360-9_9","oa_version":"Published Version","researchdata_availability":"no","corr_author":"1","series_title":"MIMB","supplementarymaterial":"no","author":[{"orcid":"0000-0002-5494-0941","full_name":"Vijatovic, David","first_name":"David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger"}],"date_updated":"2026-09-17T08:03:32Z","language":[{"iso":"eng"}],"oa":1,"pmid":1,"page":"219-229","day":"02","_id":"22926","publication":"Xenopus","OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2026-09-02T00:00:00Z","date_created":"2026-09-13T22:01:56Z","OA_type":"hybrid","publisher":"Springer","publication_status":"published","status":"public","file_date_updated":"2026-09-17T08:00:45Z","das_tickbox":"0","type":"book_chapter","title":"Adeno-Associated Virus (AAV) for tracking neural development and connectivity in Xenopus","quality_controlled":"1","month":"09","external_id":{"pmid":["42681226"]},"has_accepted_license":"1","intvolume":"      3049","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"}},{"das_tickbox":"0","type":"book_chapter","file_date_updated":"2026-09-17T07:28:53Z","title":"Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections","status":"public","quality_controlled":"1","month":"09","external_id":{"pmid":["42681228"]},"has_accepted_license":"1","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"intvolume":"      3049","keyword":["mRNA FISH","Hybridization chain reaction","In situ hybridization","Fluorescence microscopy"],"page":"245-259","day":"02","OA_place":"publisher","_id":"22925","publication":"Xenopus","OA_type":"hybrid","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2026-09-02T00:00:00Z","date_created":"2026-09-13T22:01:55Z","publisher":"Springer","publication_status":"published","editor":[{"full_name":"Beck, Caroline W.","last_name":"Beck","first_name":"Caroline W."}],"file":[{"relation":"main_file","success":1,"file_id":"22938","file_name":"2026_MIMB_Vijatovic.pdf","content_type":"application/pdf","creator":"dernst","access_level":"open_access","date_created":"2026-09-17T07:28:53Z","date_updated":"2026-09-17T07:28:53Z","file_size":1004024,"checksum":"e7a075a9ee1b91d5824f8f2b9b1f3c9b"}],"publication_identifier":{"eissn":["1940-6029"]},"fulldoi":"https://doi.org/10.1007/978-1-0716-5360-9_11","researchdata_availability":"no","corr_author":"1","oa_version":"Published Version","series_title":"MIMB","language":[{"iso":"eng"}],"supplementarymaterial":"no","date_updated":"2026-09-17T07:31:16Z","author":[{"orcid":"0000-0002-5494-0941","full_name":"Vijatovic, David","first_name":"David","id":"cf391e77-ec3c-11ea-a124-d69323410b58","last_name":"Vijatovic"},{"full_name":"Papadopoulos, Stavros","id":"40606b92-f128-11eb-9611-bf66a98cfa5c","last_name":"Papadopoulos","first_name":"Stavros"},{"first_name":"Marco","last_name":"Dalla Vecchia","id":"02a7a869-ff06-11ed-a87f-86649d6077e5","full_name":"Dalla Vecchia, Marco"},{"last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger"}],"pmid":1,"oa":1,"acknowledgement":"This work was supported by a Horizon Europe ERC Starting Grant\r\nNumber 101041551 (L.B.S., D.V., S.P.), Special Research Program\r\n(SFB) of the Austrian Science Fund (FWF) F7814-B (L.B.S., S.P.),\r\nAustrian Science Fund (FWF) 10.55776/COE16 (L.B.S.), FTI\r\nStrategy Lower Austria Dissertation Grant Number FT121-D-046\r\n(D.V.), and Austrian Academy of Sciences DOC Fellowship 27229\r\n(S.P.). We also thank the Imaging & Optics and Scientific Computing Facilities at ISTA for their support in developing the quantification pipeline.","abstract":[{"text":"Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed detection of mRNA with subcellular spatial resolution. It employs fluorophore-tagged DNA hairpins that self-assemble on target-bound probe pairs, amplifying the signal without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated image analysis pipeline that enables per-cell quantification of probe expression and co-localization. This method provides a robust and quantitative approach for visualizing gene expression patterns in situ.","lang":"eng"}],"year":"2026","scopus_import":"1","ddc":["570"],"doi":"10.1007/978-1-0716-5360-9_11","citation":{"ieee":"D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, and L. B. Sweeney, “Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections,” in <i>Xenopus</i>, vol. 3049, C. W. Beck, Ed. Springer, 2026, pp. 245–259.","apa":"Vijatovic, D., Papadopoulos, S., Dalla Vecchia, M., &#38; Sweeney, L. B. (2026). Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections. In C. W. Beck (Ed.), <i>Xenopus</i> (Vol. 3049, pp. 245–259). Springer. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">https://doi.org/10.1007/978-1-0716-5360-9_11</a>","ista":"Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. 2026.Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections. In: Xenopus. Methods in Molecular Biology, vol. 3049, 245–259.","ama":"Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections. In: Beck CW, ed. <i>Xenopus</i>. Vol 3049. MIMB. Springer; 2026:245-259. doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">10.1007/978-1-0716-5360-9_11</a>","chicago":"Vijatovic, David, Stavros Papadopoulos, Marco Dalla Vecchia, and Lora B. Sweeney. “Fluorescent in Situ MRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections.” In <i>Xenopus</i>, edited by Caroline W. Beck, 3049:245–59. MIMB. Springer, 2026. <a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">https://doi.org/10.1007/978-1-0716-5360-9_11</a>.","short":"D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, L.B. Sweeney, in:, C.W. Beck (Ed.), Xenopus, Springer, 2026, pp. 245–259.","mla":"Vijatovic, David, et al. “Fluorescent in Situ MRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections.” <i>Xenopus</i>, edited by Caroline W. Beck, vol. 3049, Springer, 2026, pp. 245–59, doi:<a href=\"https://doi.org/10.1007/978-1-0716-5360-9_11\">10.1007/978-1-0716-5360-9_11</a>."},"alternative_title":["Methods in Molecular Biology"],"article_processing_charge":"No","volume":3049,"project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e"},{"_id":"cf428362-b037-11f1-b015-8277a8a2f63d","grant_number":"COE16","name":"Neuronal circuits in health and disease (Sweeney)"},{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"},{"_id":"907b765e-16d5-11f0-9cad-fef108a945b1","grant_number":"27229","name":"A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis"}],"department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"IAS"}]}]