---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21746'
abstract:
- lang: eng
  text: As vertebrates transitioned from water to land, locomotion shifted from undulatory
    swimming to limb-based movement. How spinal circuits and their cell types evolved
    to support this transition remains unclear. We leverage frog metamorphosis, which
    recapitulates this transition within a single organism, to define how spinal circuits
    generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture
    is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons.
    As limbs develop and their movement complexifies, spinal circuits expand in neuron
    number and subtype diversity. This expansion is most pronounced for V1 inhibitory
    neurons, which increase ∼70-fold and diversify into transcriptionally distinct
    subtypes. Disrupting transcription factors defining emerging motor and V1 populations
    reveals molecular segregation between swim and limb circuits, highlighting the
    role of subtype diversity in motor coordination. A multifold increase in inhibitory
    neuron diversity thus underlies the tail-to-limb locomotor transition, providing
    a framework for spinal circuit adaptation during vertebrate evolution.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de
  Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript.
  We are also grateful to Tom Jessell and Chris Kintner for their scientific insight
  and mentorship during the conception of this project. It would also have not been
  possible without the technical support of the Aquatics and Imaging and Optics Facility
  support teams (ISTA). We thank Martin Estermann for preparing the initial draft
  of the graphical abstract and Niki Barolini for the final version. In addition,
  we thank our funding sources for providing the resources to do these experiments:
  GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon
  Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special
  Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.),
  Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858
  (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH
  grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC)
  (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472
  (to Z.H.), and Project A.L.S. (to S.B.-M.).'
article_number: '117227'
article_processing_charge: Yes
article_type: original
author:
- first_name: David
  full_name: Vijatovic, David
  id: cf391e77-ec3c-11ea-a124-d69323410b58
  last_name: Vijatovic
  orcid: 0000-0002-5494-0941
- first_name: 'Florina Alexandra '
  full_name: 'Toma, Florina Alexandra '
  id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
  last_name: Toma
- first_name: Y
  full_name: Ignatyev, Y
  last_name: Ignatyev
- first_name: Zoe P
  full_name: Harrington, Zoe P
  id: a8144562-32c9-11ee-b5ce-d9800628bda2
  last_name: Harrington
  orcid: 0009-0008-0158-4032
- first_name: Christoph M
  full_name: Sommer, Christoph M
  id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
  last_name: Sommer
  orcid: 0000-0003-1216-9105
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Matthijs Geert
  full_name: Smits, Matthijs Geert
  id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
  last_name: Smits
- first_name: Marco
  full_name: Dalla Vecchia, Marco
  id: 02a7a869-ff06-11ed-a87f-86649d6077e5
  last_name: Dalla Vecchia
- first_name: Alexandra J.
  full_name: Trevisan, Alexandra J.
  last_name: Trevisan
- first_name: Phillip
  full_name: Chapman, Phillip
  last_name: Chapman
- first_name: Mara
  full_name: Julseth, Mara
  id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
  last_name: Julseth
- first_name: Susan
  full_name: Brenner-Morton, Susan
  last_name: Brenner-Morton
- first_name: Mariano I.
  full_name: Gabitto, Mariano I.
  last_name: Gabitto
- first_name: Jeremy S.
  full_name: Dasen, Jeremy S.
  last_name: Dasen
- first_name: Jay B.
  full_name: Bikoff, Jay B.
  last_name: Bikoff
- first_name: Lora Beatrice Jaeger
  full_name: Sweeney, Lora Beatrice Jaeger
  id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
  last_name: Sweeney
  orcid: 0000-0001-9242-5601
citation:
  ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory
    cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a
    href="https://doi.org/10.1016/j.celrep.2026.117227">10.1016/j.celrep.2026.117227</a>
  apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M.,
    Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory
    cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a
    href="https://doi.org/10.1016/j.celrep.2026.117227">https://doi.org/10.1016/j.celrep.2026.117227</a>
  chicago: Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington,
    Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold
    Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell
    Reports</i>. Elsevier, 2026. <a href="https://doi.org/10.1016/j.celrep.2026.117227">https://doi.org/10.1016/j.celrep.2026.117227</a>.
  ieee: D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell
    types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier,
    2026.
  ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits
    MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto
    MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory
    cell types with emergence of limb movement. Cell Reports. 45(4), 117227.
  mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types
    with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227,
    Elsevier, 2026, doi:<a href="https://doi.org/10.1016/j.celrep.2026.117227">10.1016/j.celrep.2026.117227</a>.
  short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild,
    M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton,
    M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).
corr_author: '1'
date_created: 2026-04-19T22:07:43Z
date_published: 2026-04-28T00:00:00Z
date_updated: 2026-09-16T07:33:54Z
day: '28'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1016/j.celrep.2026.117227
external_id:
  pmid:
  - '41964955 '
file:
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  date_created: 2026-05-04T12:20:10Z
  date_updated: 2026-05-04T12:20:10Z
  file_id: '21795'
  file_name: 2026_CellReports_Vijatovic.pdf
  file_size: 14925958
  relation: main_file
  success: 1
file_date_updated: 2026-05-04T12:20:10Z
fulldoi: https://doi.org/10.1016/j.celrep.2026.117227
has_accepted_license: '1'
intvolume: '        45'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
  grant_number: '101041551'
  name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
  grant_number: F7814
  name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
    transition: cell type to connection diversity'
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
  grant_number: CZI01
  name: Tools for automation and feedback microscopy
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
  grant_number: FTI21-D-046
  name: Development of V1 interneuron diversity during swim-to-walk transition of
    Xenopus metamorphosis
- _id: cf428362-b037-11f1-b015-8277a8a2f63d
  grant_number: COE16
  name: Neuronal circuits in health and disease (Sweeney)
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '22667'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Multifold increase in spinal inhibitory cell types with emergence of limb movement
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '22926'
abstract:
- lang: eng
  text: Adeno-associated viruses (AAVs) provide a versatile tool for labeling neurons
    in the Xenopus central nervous system across developmental stages. AAV-mediated
    transgene expression is long-lasting and stable, enabling robust labeling of neuronal
    populations without genomic integration and without the need to generate transgenic
    lines. The choice of AAV capsid and promoter can restrict expression to a population
    of interest, and depending on the route and timing of delivery, AAVs can selectively
    target either progenitor-derived neuronal cohorts or mature neuronal populations.
    Here, we have outlined two labeling strategies. First, intraventricular injections
    in tadpoles transduce neural progenitor cells lining the ventricular system, resulting
    in cohort-based labeling of neurons as they differentiate during development.
    Second, direct intraparenchymal injections in post-metamorphic frogs enable spatially
    restricted labeling and anterograde or retrograde tracing of connectivity within
    defined brain regions. Together, these approaches provide selective access to
    developing and mature neural circuits in X. laevis, supporting applications ranging
    from anatomical tracing to functional imaging and manipulation of neural circuits.
acknowledgement: This work was supported by a Horizon Europe ERC Starting Grant Number
  101041551 (L.B.S. and D.V.), FTI Strategy Lower Austria Dissertation Grant Number
  FT121-D-046 (D.V.), NSF IOS Grant Number 2110086 (L.B.S. and D.V.), Special Research
  Program (SFB) of the Austrian Science Fund (FWF) F7814-B (L.B.S.), and Austrian
  Science Fund (FWF) 10.55776/COE16 (L.B.S. and D.V.).
alternative_title:
- Methods in Molecular Biology
article_processing_charge: Yes (in subscription journal)
author:
- first_name: David
  full_name: Vijatovic, David
  id: cf391e77-ec3c-11ea-a124-d69323410b58
  last_name: Vijatovic
  orcid: 0000-0002-5494-0941
- first_name: Lora Beatrice Jaeger
  full_name: Sweeney, Lora Beatrice Jaeger
  id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
  last_name: Sweeney
  orcid: 0000-0001-9242-5601
citation:
  ama: 'Vijatovic D, Sweeney LB. Adeno-Associated Virus (AAV) for tracking neural
    development and connectivity in Xenopus. In: Beck CW, ed. <i>Xenopus</i>. Vol
    3049. MIMB. Springer; 2026:219-229. doi:<a href="https://doi.org/10.1007/978-1-0716-5360-9_9">10.1007/978-1-0716-5360-9_9</a>'
  apa: Vijatovic, D., &#38; Sweeney, L. B. (2026). Adeno-Associated Virus (AAV) for
    tracking neural development and connectivity in Xenopus. In C. W. Beck (Ed.),
    <i>Xenopus</i> (Vol. 3049, pp. 219–229). Springer. <a href="https://doi.org/10.1007/978-1-0716-5360-9_9">https://doi.org/10.1007/978-1-0716-5360-9_9</a>
  chicago: Vijatovic, David, and Lora B. Sweeney. “Adeno-Associated Virus (AAV) for
    Tracking Neural Development and Connectivity in Xenopus.” In <i>Xenopus</i>, edited
    by Caroline W. Beck, 3049:219–29. MIMB. Springer, 2026. <a href="https://doi.org/10.1007/978-1-0716-5360-9_9">https://doi.org/10.1007/978-1-0716-5360-9_9</a>.
  ieee: D. Vijatovic and L. B. Sweeney, “Adeno-Associated Virus (AAV) for tracking
    neural development and connectivity in Xenopus,” in <i>Xenopus</i>, vol. 3049,
    C. W. Beck, Ed. Springer, 2026, pp. 219–229.
  ista: 'Vijatovic D, Sweeney LB. 2026.Adeno-Associated Virus (AAV) for tracking neural
    development and connectivity in Xenopus. In: Xenopus. Methods in Molecular Biology,
    vol. 3049, 219–229.'
  mla: Vijatovic, David, and Lora B. Sweeney. “Adeno-Associated Virus (AAV) for Tracking
    Neural Development and Connectivity in Xenopus.” <i>Xenopus</i>, edited by Caroline
    W. Beck, vol. 3049, Springer, 2026, pp. 219–29, doi:<a href="https://doi.org/10.1007/978-1-0716-5360-9_9">10.1007/978-1-0716-5360-9_9</a>.
  short: D. Vijatovic, L.B. Sweeney, in:, C.W. Beck (Ed.), Xenopus, Springer, 2026,
    pp. 219–229.
corr_author: '1'
das_tickbox: '0'
date_created: 2026-09-13T22:01:56Z
date_published: 2026-09-02T00:00:00Z
date_updated: 2026-09-17T08:03:32Z
day: '02'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1007/978-1-0716-5360-9_9
editor:
- first_name: Caroline W.
  full_name: Beck, Caroline W.
  last_name: Beck
external_id:
  pmid:
  - '42681226'
file:
- access_level: open_access
  checksum: ea5af1374dd6820830773e0e96535ac0
  content_type: application/pdf
  creator: dernst
  date_created: 2026-09-17T08:00:45Z
  date_updated: 2026-09-17T08:00:45Z
  file_id: '22939'
  file_name: 2026_MIMB_Vijatovic2.pdf
  file_size: 852042
  relation: main_file
  success: 1
file_date_updated: 2026-09-17T08:00:45Z
fulldoi: https://doi.org/10.1007/978-1-0716-5360-9_9
has_accepted_license: '1'
intvolume: '      3049'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1007/978-1-0716-5360-9_9
month: '09'
oa: 1
oa_version: Published Version
page: 219-229
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
  grant_number: '101041551'
  name: Development and Evolution of Tetrapod Motor Circuits
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
  grant_number: FTI21-D-046
  name: Development of V1 interneuron diversity during swim-to-walk transition of
    Xenopus metamorphosis
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
  grant_number: F7814
  name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
    transition: cell type to connection diversity'
- _id: cf428362-b037-11f1-b015-8277a8a2f63d
  grant_number: COE16
  name: Neuronal circuits in health and disease (Sweeney)
- _id: 34a02c70-11ca-11ed-8bc3-fbfd2c86c88f
  grant_number: 3(GG016346-01)
  name: Development of Viral Vectors for Amphibian Gene Delivery and Manipulation
publication: Xenopus
publication_identifier:
  eissn:
  - 1940-6029
publication_status: published
publisher: Springer
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
series_title: MIMB
status: public
supplementarymaterial: no
title: Adeno-Associated Virus (AAV) for tracking neural development and connectivity
  in Xenopus
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3049
year: '2026'
...
---
OA_place: publisher
OA_type: hybrid
_id: '22925'
abstract:
- lang: eng
  text: Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed
    detection of mRNA with subcellular spatial resolution. It employs fluorophore-tagged
    DNA hairpins that self-assemble on target-bound probe pairs, amplifying the signal
    without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence
    in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated
    image analysis pipeline that enables per-cell quantification of probe expression
    and co-localization. This method provides a robust and quantitative approach for
    visualizing gene expression patterns in situ.
acknowledgement: "This work was supported by a Horizon Europe ERC Starting Grant\r\nNumber
  101041551 (L.B.S., D.V., S.P.), Special Research Program\r\n(SFB) of the Austrian
  Science Fund (FWF) F7814-B (L.B.S., S.P.),\r\nAustrian Science Fund (FWF) 10.55776/COE16
  (L.B.S.), FTI\r\nStrategy Lower Austria Dissertation Grant Number FT121-D-046\r\n(D.V.),
  and Austrian Academy of Sciences DOC Fellowship 27229\r\n(S.P.). We also thank the
  Imaging & Optics and Scientific Computing Facilities at ISTA for their support in
  developing the quantification pipeline."
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: David
  full_name: Vijatovic, David
  id: cf391e77-ec3c-11ea-a124-d69323410b58
  last_name: Vijatovic
  orcid: 0000-0002-5494-0941
- first_name: Stavros
  full_name: Papadopoulos, Stavros
  id: 40606b92-f128-11eb-9611-bf66a98cfa5c
  last_name: Papadopoulos
- first_name: Marco
  full_name: Dalla Vecchia, Marco
  id: 02a7a869-ff06-11ed-a87f-86649d6077e5
  last_name: Dalla Vecchia
- first_name: Lora Beatrice Jaeger
  full_name: Sweeney, Lora Beatrice Jaeger
  id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
  last_name: Sweeney
  orcid: 0000-0001-9242-5601
citation:
  ama: 'Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. Fluorescent in situ
    mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus
    cryosections. In: Beck CW, ed. <i>Xenopus</i>. Vol 3049. MIMB. Springer; 2026:245-259.
    doi:<a href="https://doi.org/10.1007/978-1-0716-5360-9_11">10.1007/978-1-0716-5360-9_11</a>'
  apa: Vijatovic, D., Papadopoulos, S., Dalla Vecchia, M., &#38; Sweeney, L. B. (2026).
    Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction
    (HCR) in Xenopus cryosections. In C. W. Beck (Ed.), <i>Xenopus</i> (Vol. 3049,
    pp. 245–259). Springer. <a href="https://doi.org/10.1007/978-1-0716-5360-9_11">https://doi.org/10.1007/978-1-0716-5360-9_11</a>
  chicago: Vijatovic, David, Stavros Papadopoulos, Marco Dalla Vecchia, and Lora B.
    Sweeney. “Fluorescent in Situ MRNA Hybridization (FISH) Using Hybridization Chain
    Reaction (HCR) in Xenopus Cryosections.” In <i>Xenopus</i>, edited by Caroline
    W. Beck, 3049:245–59. MIMB. Springer, 2026. <a href="https://doi.org/10.1007/978-1-0716-5360-9_11">https://doi.org/10.1007/978-1-0716-5360-9_11</a>.
  ieee: D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, and L. B. Sweeney, “Fluorescent
    in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in
    Xenopus cryosections,” in <i>Xenopus</i>, vol. 3049, C. W. Beck, Ed. Springer,
    2026, pp. 245–259.
  ista: 'Vijatovic D, Papadopoulos S, Dalla Vecchia M, Sweeney LB. 2026.Fluorescent
    in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in
    Xenopus cryosections. In: Xenopus. Methods in Molecular Biology, vol. 3049, 245–259.'
  mla: Vijatovic, David, et al. “Fluorescent in Situ MRNA Hybridization (FISH) Using
    Hybridization Chain Reaction (HCR) in Xenopus Cryosections.” <i>Xenopus</i>, edited
    by Caroline W. Beck, vol. 3049, Springer, 2026, pp. 245–59, doi:<a href="https://doi.org/10.1007/978-1-0716-5360-9_11">10.1007/978-1-0716-5360-9_11</a>.
  short: D. Vijatovic, S. Papadopoulos, M. Dalla Vecchia, L.B. Sweeney, in:, C.W.
    Beck (Ed.), Xenopus, Springer, 2026, pp. 245–259.
corr_author: '1'
das_tickbox: '0'
date_created: 2026-09-13T22:01:55Z
date_published: 2026-09-02T00:00:00Z
date_updated: 2026-09-17T07:31:16Z
day: '02'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: IAS
doi: 10.1007/978-1-0716-5360-9_11
editor:
- first_name: Caroline W.
  full_name: Beck, Caroline W.
  last_name: Beck
external_id:
  pmid:
  - '42681228'
file:
- access_level: open_access
  checksum: e7a075a9ee1b91d5824f8f2b9b1f3c9b
  content_type: application/pdf
  creator: dernst
  date_created: 2026-09-17T07:28:53Z
  date_updated: 2026-09-17T07:28:53Z
  file_id: '22938'
  file_name: 2026_MIMB_Vijatovic.pdf
  file_size: 1004024
  relation: main_file
  success: 1
file_date_updated: 2026-09-17T07:28:53Z
fulldoi: https://doi.org/10.1007/978-1-0716-5360-9_11
has_accepted_license: '1'
intvolume: '      3049'
keyword:
- mRNA FISH
- Hybridization chain reaction
- In situ hybridization
- Fluorescence microscopy
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 245-259
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
  grant_number: '101041551'
  name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
  grant_number: F7814
  name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
    transition: cell type to connection diversity'
- _id: cf428362-b037-11f1-b015-8277a8a2f63d
  grant_number: COE16
  name: Neuronal circuits in health and disease (Sweeney)
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
  grant_number: FTI21-D-046
  name: Development of V1 interneuron diversity during swim-to-walk transition of
    Xenopus metamorphosis
- _id: 907b765e-16d5-11f0-9cad-fef108a945b1
  grant_number: '27229'
  name: 'A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb
    transition of Xenopus metamorphosis'
publication: Xenopus
publication_identifier:
  eissn:
  - 1940-6029
publication_status: published
publisher: Springer
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
series_title: MIMB
status: public
supplementarymaterial: no
title: Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction
  (HCR) in Xenopus cryosections
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3049
year: '2026'
...