[{"abstract":[{"lang":"eng","text":"Bacterial ion fluxes are involved in the generation of energy, transport, and motility. As such, bacterial electrophysiology is fundamentally important for the bacterial life cycle, but it is often neglected and consequently, by and large, not understood. Arguably, the two main reasons for this are the complexity of measuring relevant variables in small cells with a cell envelope that contains the cell wall and the fact that, in a unicellular organism, relevant variables become intertwined in a nontrivial manner. To help give bacterial electrophysiology studies a firm footing, in this review, we go back to basics. We look first at the biophysics of bacterial membrane potential, and then at the approaches and models developed mostly for the study of neurons and eukaryotic mitochondria. We discuss their applicability to bacterial cells. Finally, we connect bacterial membrane potential with other relevant (electro)physiological variables and summarize methods that can be used to both measure and influence bacterial electrophysiology."}],"file":[{"access_level":"open_access","relation":"main_file","date_created":"2024-07-29T10:56:01Z","file_name":"2024_AnnualReviewBiophys_Lo.pdf","checksum":"e0505553b3cee624fa865f0cc5a99ecc","content_type":"application/pdf","success":1,"creator":"dernst","file_id":"17339","file_size":1276645,"date_updated":"2024-07-29T10:56:01Z"}],"doi":"10.1146/annurev-biophys-030822-032215","ddc":["570"],"oa_version":"Published Version","publisher":"Annual Reviews","article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"intvolume":"        53","citation":{"ista":"Lo WC, Krasnopeeva E, Pilizota T. 2024. Bacterial Electrophysiology. Annual Review of Biophysics. 53, 487–510.","short":"W.C. Lo, E. Krasnopeeva, T. Pilizota, Annual Review of Biophysics 53 (2024) 487–510.","chicago":"Lo, Wei Chang, Ekaterina Krasnopeeva, and Teuta Pilizota. “Bacterial Electrophysiology.” <i>Annual Review of Biophysics</i>. Annual Reviews, 2024. <a href=\"https://doi.org/10.1146/annurev-biophys-030822-032215\">https://doi.org/10.1146/annurev-biophys-030822-032215</a>.","ama":"Lo WC, Krasnopeeva E, Pilizota T. Bacterial Electrophysiology. <i>Annual Review of Biophysics</i>. 2024;53:487-510. doi:<a href=\"https://doi.org/10.1146/annurev-biophys-030822-032215\">10.1146/annurev-biophys-030822-032215</a>","ieee":"W. C. Lo, E. Krasnopeeva, and T. Pilizota, “Bacterial Electrophysiology,” <i>Annual Review of Biophysics</i>, vol. 53. Annual Reviews, pp. 487–510, 2024.","apa":"Lo, W. C., Krasnopeeva, E., &#38; Pilizota, T. (2024). Bacterial Electrophysiology. <i>Annual Review of Biophysics</i>. Annual Reviews. <a href=\"https://doi.org/10.1146/annurev-biophys-030822-032215\">https://doi.org/10.1146/annurev-biophys-030822-032215</a>","mla":"Lo, Wei Chang, et al. “Bacterial Electrophysiology.” <i>Annual Review of Biophysics</i>, vol. 53, Annual Reviews, 2024, pp. 487–510, doi:<a href=\"https://doi.org/10.1146/annurev-biophys-030822-032215\">10.1146/annurev-biophys-030822-032215</a>."},"author":[{"last_name":"Lo","full_name":"Lo, Wei Chang","first_name":"Wei Chang"},{"last_name":"Krasnopeeva","full_name":"Krasnopeeva, Ekaterina","id":"1F1EE44A-BF83-11EA-B3C1-BB9CC619BF3A","first_name":"Ekaterina"},{"last_name":"Pilizota","full_name":"Pilizota, Teuta","first_name":"Teuta"}],"year":"2024","date_published":"2024-07-01T00:00:00Z","pmid":1,"page":"487-510","oa":1,"volume":53,"quality_controlled":"1","publication_identifier":{"issn":["1936-122X"],"eissn":["1936-1238"]},"acknowledgement":"We would like to thank all members of the Pilizota lab, as well as Calin Guet, Orkun Soyer, Munehiro Asally, Peter Swain, and in particular Matt Scott and Ariel Amir, for their support, comments, and useful discussions. T.P. and W.-C.L. were supported by the Leverhulme Trust, grant RPG-2019-187, and T.P. is supported by EPSRC Fellowship EP/V03264X/1. E.K. was supported by a European Molecular Biology Organization Long-Term Postdoctoral Fellowship, ALTF 44-2021.","external_id":{"isi":["001278237500021"],"pmid":["38382113"]},"scopus_import":"1","month":"07","_id":"17325","file_date_updated":"2024-07-29T10:56:01Z","publication":"Annual Review of Biophysics","title":"Bacterial Electrophysiology","license":"https://creativecommons.org/licenses/by/4.0/","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","isi":1,"date_updated":"2025-09-08T08:34:43Z","project":[{"name":"Bacterial cytoplasm glass transition: passive physiological switch or active survival strategy","_id":"eb872896-77a9-11ec-83b8-f59a38ec17f8","grant_number":"ALTF 44-2021"}],"date_created":"2024-07-28T22:01:09Z","day":"01","publication_status":"published","article_type":"original","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"CaGu"}]}]