[{"department":[{"_id":"SiHi"},{"_id":"LoSw"}],"status":"public","title":"Lineage origin of spinal cord cell type diversity","type":"preprint","month":"02","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"_id":"21291","citation":{"chicago":"Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B. Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>.","mla":"Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>.","ama":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>","ieee":"S. A. Gobeil <i>et al.</i>, “Lineage origin of spinal cord cell type diversity,” <i>bioRxiv</i>. .","apa":"Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher, C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell type diversity. <i>bioRxiv</i>. <a href=\"https://doi.org/10.64898/2026.02.12.705305\">https://doi.org/10.64898/2026.02.12.705305</a>","ista":"Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity. bioRxiv, <a href=\"https://doi.org/10.64898/2026.02.12.705305\">10.64898/2026.02.12.705305</a>.","short":"S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher, G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.)."},"date_updated":"2026-04-14T08:16:55Z","year":"2026","acknowledgement":"We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko, and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB consortium for comments on\r\nthe manuscript. We are also grateful for the technical support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA). In addition, we thank our funding sources for providing\r\nthe resources to do these experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.); Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C. by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S. and G.C. were\r\nadditionally supported by institutional funds from the ISTA and the University of Exeter,\r\nrespectively. ","publication":"bioRxiv","article_processing_charge":"No","date_published":"2026-02-16T00:00:00Z","doi":"10.64898/2026.02.12.705305","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"abstract":[{"lang":"eng","text":"The complexity and specificity of movement in vertebrates is driven by a rich diversity of spinal motor and interneuron cell types. During development, eleven spinal cord progenitor domains generate an equivalent number of cardinal neuron types. How progenitor domains, individual progenitors, and post-mitotic diversity relate is still unknown. We performed high-resolution, single-progenitor cell lineage tracing in the embryonic mouse spinal cord using mosaic analysis with double markers (MADM). Our quantitative study of lineage progression revealed that spinal cord progenitors undergo highly variable numbers of proliferative, neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons migrate radially over large distances, span the dorsoventral axis, and even cross the midline, demonstrating striking bilaterality. Molecular and morphometric analysis indicate high levels of progenitor multipotency, with an individual progenitor capable of producing several molecularly and morphologically distinct neuron types, as well as astrocytes. These findings redefine spinal cord development as a process in which lineage variability — rather than rigid progenitor identity — drives the generation of cellular diversity."}],"corr_author":"1","main_file_link":[{"url":"https://doi.org/10.64898/2026.02.12.705305","open_access":"1"}],"date_created":"2026-02-17T11:36:20Z","oa_version":"Preprint","project":[{"name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","grant_number":"101041551"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression","_id":"059F6AB4-7A3F-11EA-A408-12923DDC885E","grant_number":"F7805"}],"ddc":["570"],"has_accepted_license":"1","OA_type":"green","publication_status":"submitted","author":[{"id":"2f3e9efb-eb24-11ec-86b2-88efb11d59fa","full_name":"Gobeil, Sophie A","first_name":"Sophie A","last_name":"Gobeil"},{"id":"8cfb7412-10a7-11f1-add1-82b44e6418f2","last_name":"Da Silveira Neto","first_name":"Francisco","full_name":"Da Silveira Neto, Francisco"},{"last_name":"Silvestrelli","first_name":"Giulia","full_name":"Silvestrelli, Giulia","id":"12632ae8-799e-11ef-94a2-e5a3b5ef49e9"},{"id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","last_name":"Smits","first_name":"Matthijs Geert","full_name":"Smits, Matthijs Geert"},{"id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher","full_name":"Streicher, Carmen","first_name":"Carmen"},{"orcid":"0000-0001-8457-2572","id":"471195F6-F248-11E8-B48F-1D18A9856A87","first_name":"Giselle T","full_name":"Cheung, Giselle T","last_name":"Cheung"},{"first_name":"Simon","full_name":"Hippenmeyer, Simon","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","id":"37B36620-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"repository","oa":1,"day":"16","language":[{"iso":"eng"}]},{"citation":{"apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>","ieee":"D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier, 2026.","short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227.","chicago":"Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>.","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>.","ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>"},"file_date_updated":"2026-05-04T12:20:10Z","date_updated":"2026-05-04T12:27:06Z","article_type":"original","issue":"4","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"quality_controlled":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"21746","publisher":"Elsevier","type":"journal_article","month":"04","status":"public","title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","publication_status":"published","OA_type":"gold","has_accepted_license":"1","author":[{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","last_name":"Vijatovic","first_name":"David","full_name":"Vijatovic, David"},{"last_name":"Toma","first_name":"Florina Alexandra ","full_name":"Toma, Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e"},{"last_name":"Ignatyev","full_name":"Ignatyev, Y","first_name":"Y"},{"id":"a8144562-32c9-11ee-b5ce-d9800628bda2","orcid":"0009-0008-0158-4032","last_name":"Harrington","full_name":"Harrington, Zoe P","first_name":"Zoe P"},{"last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105"},{"last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"last_name":"Smits","full_name":"Smits, Matthijs Geert","first_name":"Matthijs Geert","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0"},{"last_name":"Dalla Vecchia","first_name":"Marco","full_name":"Dalla Vecchia, Marco","id":"02a7a869-ff06-11ed-a87f-86649d6077e5"},{"last_name":"Trevisan","first_name":"Alexandra J.","full_name":"Trevisan, Alexandra J."},{"first_name":"Phillip","full_name":"Chapman, Phillip","last_name":"Chapman"},{"id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","last_name":"Julseth","first_name":"Mara","full_name":"Julseth, Mara"},{"first_name":"Susan","full_name":"Brenner-Morton, Susan","last_name":"Brenner-Morton"},{"first_name":"Mariano I.","full_name":"Gabitto, Mariano I.","last_name":"Gabitto"},{"full_name":"Dasen, Jeremy S.","first_name":"Jeremy S.","last_name":"Dasen"},{"first_name":"Jay B.","full_name":"Bikoff, Jay B.","last_name":"Bikoff"},{"full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425"}],"scopus_import":"1","external_id":{"pmid":["41964955 "]},"language":[{"iso":"eng"}],"day":"28","oa":1,"publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"OA_place":"publisher","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"file_id":"21795","success":1,"relation":"main_file","file_size":14925958,"date_created":"2026-05-04T12:20:10Z","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef","creator":"dernst","access_level":"open_access","content_type":"application/pdf","file_name":"2026_CellReports_Vijatovic.pdf","date_updated":"2026-05-04T12:20:10Z"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"doi":"10.1016/j.celrep.2026.117227","publication":"Cell Reports","article_processing_charge":"Yes","date_published":"2026-04-28T00:00:00Z","abstract":[{"lang":"eng","text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution."}],"year":"2026","intvolume":"        45","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","article_number":"117227","volume":45,"oa_version":"Published Version","ddc":["570"],"DOAJ_listed":"1","project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814"},{"grant_number":"CZI01","name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"},{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046"}],"corr_author":"1","PlanS_conform":"1","date_created":"2026-04-19T22:07:43Z"},{"year":"2025","acknowledgement":"We would like to thank the members of the Sweeney Lab for discussion and support; Andrey\r\nBydanov for technical assistance with single-cell sequencing processing; and Jay Bikoff,\r\nNikos Konstantinides, Maria Tosches, and Graziana Gatto for comments on the manuscript. \r\nThis research was supported by: Horizon Europe ERC Starting Grant 101041551 (L.B.S,\r\nY.I., S.P.); Special Research Program (SFB) of the Austrian Science Fund (FWF) F7814-B\r\n(L.B.S., S.P., E.M.T); Austrian Science Fund (FWF) 10.55776/COE16 (L.B.S., Y.I., E.M.T.);\r\nAustrian Academy of Sciences DOC Fellowship 27229 (S.P.); ERC Advanced Grant 742046\r\n(E.M.T.); NIH award R24 OD031956 (L.P.); and in part by the Intramural Research\r\nProgram of the National Institutes of Health (NIH) through 1ZIA NS003153 to A.J.L.\r\nThe contributions of the NIH author are considered Works of the United States\r\nGovernment. The findings and conclusions presented in this paper are those of\r\nthe authors and do not necessarily reflect the views of the NIH or the U.S. Department\r\nof Health and Human Services. ","date_published":"2025-10-11T00:00:00Z","publication":"bioRxiv","article_processing_charge":"No","doi":"10.1101/2025.10.09.680955","abstract":[{"text":"Vertebrates display remarkable diversity of sensorimotor behaviors, each adapted to distinct ecological and survival demands. This diversity raises fundamental questions about the evolutionary origin of motor control: do conserved spinal circuits underlie these behaviors, and how have they diverged across species. Recent studies detail spinal cell-type architecture in mammals but comparable, high-resolution atlases of the non-mammalian spinal cord are lacking. Here, we compare spinal cord cell types between fish, frogs, mice and humans, spanning ∼450 million years of evolution. Across species, we define highly conserved programs of cell type specification that segregate spinal neurons into nearly identical cardinal classes during development. This contrasts with adult stages, when spinal cell-type composition selectively diverges for excitatory neuron subpopulations. Using spatial transcriptomics, we localize this species divergence to the superficial, dorsal spinal cord, where variant neuropeptide expression defines mammalian-specific cell types. The most dorsal spinal cord thus emerges as a recently evolved hub for sensory integration in mammals, a neospinal cord analogous to the neocortex.</jats:p>","lang":"eng"}],"department":[{"_id":"LoSw"},{"_id":"ScienComp"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.10.09.680955"}],"corr_author":"1","title":"Innovations in spinal cord cell type heterogeneity across vertebrate evolution","date_created":"2026-05-27T06:54:04Z","status":"public","type":"preprint","month":"10","oa_version":"Preprint","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"project":[{"grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits"},{"grant_number":"27229","name":"A Tale of Two Circuits: Rostrocaudal spinal cord patterning during the swim-to-limb transition of Xenopus metamorphosis","_id":"907b765e-16d5-11f0-9cad-fef108a945b1"}],"_id":"21920","citation":{"chicago":"Ignatyev, Yuri, Stavros Papadopoulos, Mateja Soretić, Jake Yeung, Tzi-Yang Lin, Elly M Tanaka, Leonid Peshkin, Ariel J Levine, Mariano I Gabitto, and Lora B. Sweeney. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.09.680955\">https://doi.org/10.1101/2025.10.09.680955</a>.","ama":"Ignatyev Y, Papadopoulos S, Soretić M, et al. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.09.680955\">10.1101/2025.10.09.680955</a>","mla":"Ignatyev, Yuri, et al. “Innovations in Spinal Cord Cell Type Heterogeneity across Vertebrate Evolution.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.09.680955\">10.1101/2025.10.09.680955</a>.","apa":"Ignatyev, Y., Papadopoulos, S., Soretić, M., Yeung, J., Lin, T.-Y., Tanaka, E. M., … Sweeney, L. B. (n.d.). Innovations in spinal cord cell type heterogeneity across vertebrate evolution. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.09.680955\">https://doi.org/10.1101/2025.10.09.680955</a>","ieee":"Y. Ignatyev <i>et al.</i>, “Innovations in spinal cord cell type heterogeneity across vertebrate evolution,” <i>bioRxiv</i>. .","short":"Y. Ignatyev, S. Papadopoulos, M. Soretić, J. Yeung, T.-Y. Lin, E.M. Tanaka, L. Peshkin, A.J. Levine, M.I. Gabitto, L.B. Sweeney, BioRxiv (n.d.).","ista":"Ignatyev Y, Papadopoulos S, Soretić M, Yeung J, Lin T-Y, Tanaka EM, Peshkin L, Levine AJ, Gabitto MI, Sweeney LB. Innovations in spinal cord cell type heterogeneity across vertebrate evolution. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.09.680955\">10.1101/2025.10.09.680955</a>."},"publication_status":"submitted","OA_type":"green","date_updated":"2026-05-27T07:25:41Z","author":[{"last_name":"Ignatyev","full_name":"Ignatyev, Yuri","first_name":"Yuri"},{"first_name":"Stavros","full_name":"Papadopoulos, Stavros","last_name":"Papadopoulos","id":"40606b92-f128-11eb-9611-bf66a98cfa5c"},{"last_name":"Soretić","full_name":"Soretić, Mateja","first_name":"Mateja"},{"first_name":"Jake","full_name":"Yeung, Jake","last_name":"Yeung","orcid":"0000-0003-1732-1559","id":"123012b2-db30-11eb-b4d8-a35840c0551b"},{"full_name":"Lin, Tzi-Yang","first_name":"Tzi-Yang","last_name":"Lin"},{"full_name":"Tanaka, Elly M","first_name":"Elly M","last_name":"Tanaka"},{"full_name":"Peshkin, Leonid","first_name":"Leonid","last_name":"Peshkin"},{"last_name":"Levine","first_name":"Ariel J","full_name":"Levine, Ariel J"},{"first_name":"Mariano I","full_name":"Gabitto, Mariano I","last_name":"Gabitto"},{"last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","OA_place":"repository","oa":1,"day":"11","language":[{"iso":"eng"}]},{"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"doi":"10.1016/j.devcel.2024.10.025","date_published":"2025-03-10T00:00:00Z","publication":"Developmental Cell","article_processing_charge":"Yes (via OA deal)","abstract":[{"lang":"eng","text":"Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although successful in mammals, AAVs have never been shown to transduce amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and identified at least two AAV serotypes per species that transduce neurons. In developing amphibians, AAVs labeled groups of neurons generated at the same time during development. In the mature brain, AAVrg retrogradely traced long-range projections. Our study introduces AAVs as a tool for amphibian research, establishes a generalizable workflow for AAV screening in new species, and expands opportunities for cross-species comparisons of nervous system development, function, and evolution."}],"intvolume":"        60","year":"2025","acknowledgement":"We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion, and support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru (Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination; our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC, and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.); FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.); EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).","oa_version":"Published Version","volume":60,"ddc":["570"],"project":[{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046"},{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814"}],"corr_author":"1","date_created":"2024-02-20T09:20:32Z","OA_type":"hybrid","publication_status":"published","has_accepted_license":"1","author":[{"last_name":"Jaeger","full_name":"Jaeger, Eliza C.B.","first_name":"Eliza C.B."},{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","full_name":"Vijatovic, David","first_name":"David","last_name":"Vijatovic"},{"full_name":"Deryckere, Astrid","first_name":"Astrid","last_name":"Deryckere"},{"full_name":"Zorin, Nikol","first_name":"Nikol","last_name":"Zorin"},{"last_name":"Nguyen","full_name":"Nguyen, Akemi L.","first_name":"Akemi L."},{"id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","last_name":"Ivanian","first_name":"Georgiy","full_name":"Ivanian, Georgiy"},{"first_name":"Jamie","full_name":"Woych, Jamie","last_name":"Woych"},{"last_name":"Arnold","first_name":"Rebecca C","full_name":"Arnold, Rebecca C","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f"},{"full_name":"Ortega Gurrola, Alonso","first_name":"Alonso","last_name":"Ortega Gurrola"},{"last_name":"Shvartsman","full_name":"Shvartsman, Arik","first_name":"Arik"},{"full_name":"Barbieri, Francesca","first_name":"Francesca","last_name":"Barbieri","id":"a9492887-8972-11ed-ae7b-bfae10998254"},{"full_name":"Toma, Florina-Alexandra","first_name":"Florina-Alexandra","last_name":"Toma","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5"},{"first_name":"Gary J.","full_name":"Gorbsky, Gary J.","last_name":"Gorbsky"},{"last_name":"Horb","full_name":"Horb, Marko E.","first_name":"Marko E."},{"last_name":"Cline","full_name":"Cline, Hollis T.","first_name":"Hollis T."},{"last_name":"Shay","full_name":"Shay, Timothy F.","first_name":"Timothy F."},{"first_name":"Darcy B.","full_name":"Kelley, Darcy B.","last_name":"Kelley"},{"full_name":"Yamaguchi, Ayako","first_name":"Ayako","last_name":"Yamaguchi"},{"last_name":"Shein-Idelson","full_name":"Shein-Idelson, Mark","first_name":"Mark"},{"full_name":"Tosches, Maria Antonietta","first_name":"Maria Antonietta","last_name":"Tosches"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger"}],"scopus_import":"1","external_id":{"isi":["001444798600001"],"pmid":["39603234"]},"language":[{"iso":"eng"}],"day":"10","OA_place":"publisher","pmid":1,"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"oa":1,"file":[{"date_created":"2025-06-04T05:43:27Z","relation":"main_file","file_size":11936258,"file_id":"19790","success":1,"content_type":"application/pdf","file_name":"2025_DevelopmentalCell_Jaeger.pdf","access_level":"open_access","date_updated":"2025-06-04T05:43:27Z","creator":"dernst","checksum":"a83a4cb58f5941096d3ad91ca0172594"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"quality_controlled":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"_id":"15016","isi":1,"publisher":"Elsevier","month":"03","type":"journal_article","title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians","status":"public","page":"794-812.e6","citation":{"chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>.","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. 2025;60(5):794-812.e6. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>","mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>, vol. 60, no. 5, Elsevier, 2025, p. 794–812.e6, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>.","ieee":"E. C. B. Jaeger <i>et al.</i>, “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” <i>Developmental Cell</i>, vol. 60, no. 5. Elsevier, p. 794–812.e6, 2025.","apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>","ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6."},"file_date_updated":"2025-06-04T05:43:27Z","date_updated":"2025-09-30T10:00:55Z","article_type":"original","issue":"5"},{"citation":{"short":"D. Vijatovic, F.A. Toma, Z.P. Harrington, C.M. Sommer, R. Hauschild, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, BioRxiv (n.d.).","ista":"Vijatovic D, Toma FA, Harrington ZP, Sommer CM, Hauschild R, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. bioRxiv, <a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","apa":"Vijatovic, D., Toma, F. A., Harrington, Z. P., Sommer, C. M., Hauschild, R., Trevisan, A. J., … Sweeney, L. B. (n.d.). Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>","ieee":"D. Vijatovic <i>et al.</i>, “Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis,” <i>bioRxiv</i>. .","mla":"Vijatovic, David, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>.","ama":"Vijatovic D, Toma FA, Harrington ZP, et al. Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.09.20.614050\">10.1101/2024.09.20.614050</a>","chicago":"Vijatovic, David, Florina Alexandra  Toma, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Alexandra J. Trevisan, Phillip Chapman, et al. “Spinal Neuron Diversity Scales Exponentially with Swim-to-Limb Transformation during Frog Metamorphosis.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.09.20.614050\">https://doi.org/10.1101/2024.09.20.614050</a>."},"publication_status":"submitted","OA_type":"green","date_updated":"2025-05-14T11:40:13Z","author":[{"first_name":"David","full_name":"Vijatovic, David","last_name":"Vijatovic","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","full_name":"Toma, Florina Alexandra ","first_name":"Florina Alexandra ","last_name":"Toma"},{"first_name":"Zoe P","full_name":"Harrington, Zoe P","last_name":"Harrington","orcid":"0009-0008-0158-4032","id":"a8144562-32c9-11ee-b5ce-d9800628bda2"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"last_name":"Trevisan","first_name":"Alexandra J.","full_name":"Trevisan, Alexandra J."},{"first_name":"Phillip","full_name":"Chapman, Phillip","last_name":"Chapman"},{"full_name":"Julseth, Mara","first_name":"Mara","last_name":"Julseth","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1"},{"full_name":"Brenner-Morton, Susan","first_name":"Susan","last_name":"Brenner-Morton"},{"first_name":"Mariano I.","full_name":"Gabitto, Mariano I.","last_name":"Gabitto"},{"full_name":"Dasen, Jeremy S.","first_name":"Jeremy S.","last_name":"Dasen"},{"last_name":"Bikoff","full_name":"Bikoff, Jay B.","first_name":"Jay B."},{"orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","full_name":"Sweeney, Lora Beatrice Jaeger","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"OA_place":"repository","language":[{"iso":"eng"}],"day":"27","year":"2024","acknowledgement":"We would like to thank the members of the Sweeney Lab (especially Stavros Papadopoulos and\r\nSophie Gobeil) for their contributions to this project and, in addition to the lab, Graziana Gatto\r\nand Mario de Bono, for discussion, and support. We are also grateful to Tom Jessell and Chris\r\nKintner for their scientific insight and mentorship during the conception of this project. This\r\nproject would also not have been possible with the technical support of the Matthias Nowak,\r\nVerena Mayer and the Aquatics as well as the Imaging and Optics Facility support teams\r\n(ISTA). In addition, we thank our funding sources for providing the resources to do these\r\nexperiments: FTI Strategy Lower Austria Dissertation Grant Number FT121-D-046 (D.V.);\r\nHorizon Europe ERC Starting Grant Number 101041551 (L.B.S., F.A.T. and D.V); Special\r\nResearch Program (SFB) of the Austrian Science Fund (FWF) Project number F7814-B (L.B.S);\r\nNINDS 5R35NS116858 (J.S.D); CZI grant DAF2020-225401 (DOI): 10.37921/120055ratwvi\r\n(R.H.); NIH grant number R01NS123116 (J.B.B); American Lebanese Syrian Associated\r\nCharities (ALSAC) (J.B.B.); German Academic Exchange Service (DAAD) IFI Grant Number\r\n57515251-91853472 (Z.H.); and Project A.L.S. (S.B-M.). ","date_published":"2024-09-27T00:00:00Z","article_processing_charge":"No","publication":"bioRxiv","doi":"10.1101/2024.09.20.614050","acknowledged_ssus":[{"_id":"Bio"}],"abstract":[{"lang":"eng","text":"Vertebrates exhibit a wide range of motor behaviors, ranging from swimming to complex limb-based movements. Here we take advantage of frog metamorphosis, which captures a swim-to-limb-based movement transformation during the development of a single organism, to explore changes in the underlying spinal circuits. We find that the tadpole spinal cord contains small and largely homogeneous populations of motor neurons (MNs) and V1 interneurons (V1s) at early escape swimming stages. These neuronal populations only modestly increase in number and subtype heterogeneity with the emergence of free swimming. In contrast, during frog metamorphosis and the emergence of limb movement, there is a dramatic expansion of MN and V1 interneuron number and transcriptional heterogeneity, culminating in cohorts of neurons that exhibit striking molecular similarity to mammalian motor circuits. CRISPR/Cas9-mediated gene disruption of the limb MN and V1 determinants FoxP1 and Engrailed-1, respectively, results in severe but selective deficits in tail and limb function. Our work thus demonstrates that neural diversity scales exponentially with increasing behavioral complexity and illustrates striking evolutionary conservation in the molecular organization and function of motor circuits across species."}],"department":[{"_id":"LoSw"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"corr_author":"1","main_file_link":[{"url":"https://doi.org/10.1101/2024.09.20.614050","open_access":"1"}],"status":"public","date_created":"2025-04-07T08:48:28Z","title":"Spinal neuron diversity scales exponentially with swim-to-limb transformation during frog metamorphosis","month":"09","type":"preprint","oa_version":"Preprint","project":[{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046"},{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551"},{"name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","grant_number":"CZI01"}],"_id":"19520"},{"article_type":"original","issue":"40","citation":{"apa":"Kratsios, P., Zampieri, N., Carrillo, R., Mizumoto, K., Sweeney, L. B., &#38; Philippidou, P. (2024). Molecular and cellular mechanisms of motor circuit development. <i>The Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">https://doi.org/10.1523/JNEUROSCI.1238-24.2024</a>","ieee":"P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L. B. Sweeney, and P. Philippidou, “Molecular and cellular mechanisms of motor circuit development,” <i>The Journal of Neuroscience</i>, vol. 44, no. 40. Society for Neuroscience, 2024.","short":"P. Kratsios, N. Zampieri, R. Carrillo, K. Mizumoto, L.B. Sweeney, P. Philippidou, The Journal of Neuroscience 44 (2024).","ista":"Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P. 2024. Molecular and cellular mechanisms of motor circuit development. The Journal of Neuroscience. 44(40), e1238242024.","chicago":"Kratsios, Paschalis, Niccolò Zampieri, Robert Carrillo, Kota Mizumoto, Lora B. Sweeney, and Polyxeni Philippidou. “Molecular and Cellular Mechanisms of Motor Circuit Development.” <i>The Journal of Neuroscience</i>. Society for Neuroscience, 2024. <a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">https://doi.org/10.1523/JNEUROSCI.1238-24.2024</a>.","ama":"Kratsios P, Zampieri N, Carrillo R, Mizumoto K, Sweeney LB, Philippidou P. Molecular and cellular mechanisms of motor circuit development. <i>The Journal of Neuroscience</i>. 2024;44(40). doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">10.1523/JNEUROSCI.1238-24.2024</a>","mla":"Kratsios, Paschalis, et al. “Molecular and Cellular Mechanisms of Motor Circuit Development.” <i>The Journal of Neuroscience</i>, vol. 44, no. 40, e1238242024, Society for Neuroscience, 2024, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1238-24.2024\">10.1523/JNEUROSCI.1238-24.2024</a>."},"date_updated":"2026-01-05T14:01:26Z","publisher":"Society for Neuroscience","title":"Molecular and cellular mechanisms of motor circuit development","status":"public","type":"journal_article","month":"10","OA_embargo":"6 months","isi":1,"_id":"18305","quality_controlled":"1","department":[{"_id":"LoSw"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"OA_place":"publisher","oa":1,"publication_identifier":{"eissn":["1529-2401"]},"language":[{"iso":"eng"}],"day":"02","has_accepted_license":"1","publication_status":"published","OA_type":"hybrid","external_id":{"isi":["001335212200016"],"pmid":["39358025"]},"scopus_import":"1","author":[{"last_name":"Kratsios","first_name":"Paschalis","full_name":"Kratsios, Paschalis"},{"full_name":"Zampieri, Niccolò","first_name":"Niccolò","last_name":"Zampieri"},{"first_name":"Robert","full_name":"Carrillo, Robert","last_name":"Carrillo"},{"full_name":"Mizumoto, Kota","first_name":"Kota","last_name":"Mizumoto"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","orcid":"0000-0001-9242-5601","last_name":"Sweeney","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger"},{"last_name":"Philippidou","full_name":"Philippidou, Polyxeni","first_name":"Polyxeni"}],"main_file_link":[{"url":"https://doi.org/10.1523/JNEUROSCI.1238-24.2024","open_access":"1"}],"date_created":"2024-10-13T22:01:49Z","oa_version":"Published Version","volume":44,"article_number":"e1238242024","ddc":["570"],"project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551"}],"year":"2024","intvolume":"        44","acknowledgement":"Work in the authors’ labs is funded by the Helmholtz Association (N.Z.), National Institute of Neurological Disorders and Stroke (NINDS) R01NS116365 (P.K.), NINDS R01NS123439 and National Science Foundation IOS-2048080 (R.C.), NINDS R01NS114510 (P.P.), Natural Sciences and Engineering Research Council of Canada RGPIN-2021-03154 (K.M.) and Horizon Europe European Research Council Starting Grant Number 101041551 (L.B.S.). P.P. is the Weidenthal Family Designated Professor in Career Development.","article_processing_charge":"No","publication":"The Journal of Neuroscience","date_published":"2024-10-02T00:00:00Z","doi":"10.1523/JNEUROSCI.1238-24.2024","abstract":[{"lang":"eng","text":"Motor circuits represent the main output of the central nervous system and produce dynamic behaviors ranging from relatively simple rhythmic activities like swimming in fish and breathing in mammals to highly sophisticated dexterous movements in humans. Despite decades of research, the development and function of motor circuits remain poorly understood. Breakthroughs in the field recently provided new tools and tractable model systems that set the stage to discover the molecular mechanisms and circuit logic underlying motor control. Here, we describe recent advances from both vertebrate (mouse, frog) and invertebrate (nematode, fruit fly) systems on cellular and molecular mechanisms that enable motor circuits to develop and function and highlight conserved and divergent mechanisms necessary for motor circuit development."}]},{"language":[{"iso":"eng"}],"day":"26","pmid":1,"publication_identifier":{"issn":["1662-5110"]},"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"date_updated":"2024-01-03T13:33:21Z","content_type":"application/pdf","file_name":"2023_FrontiersNeuralCircuits_Wilson.pdf","access_level":"open_access","creator":"dernst","checksum":"7efd06de284a28e91e97127611a9c3fd","date_created":"2024-01-03T13:33:21Z","file_size":6667157,"relation":"main_file","success":1,"file_id":"14729"}],"author":[{"id":"5230e794-15b2-11ec-abd3-e2d5335ebd1d","orcid":"0000-0001-6191-1367","last_name":"Wilson","full_name":"Wilson, Alexia C","first_name":"Alexia C"},{"orcid":"0000-0001-9242-5601","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","full_name":"Sweeney, Lora Beatrice Jaeger","last_name":"Sweeney"}],"external_id":{"pmid":["37180760"],"isi":["000984606200001"]},"scopus_import":"1","publication_status":"published","has_accepted_license":"1","ddc":["570"],"project":[{"_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae","name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551"}],"article_number":"1146449","oa_version":"Published Version","volume":17,"date_created":"2023-05-28T22:01:04Z","corr_author":"1","abstract":[{"text":"Vertebrate movement is orchestrated by spinal inter- and motor neurons that, together with sensory and cognitive input, produce dynamic motor behaviors. These behaviors vary from the simple undulatory swimming of fish and larval aquatic species to the highly coordinated running, reaching and grasping of mice, humans and other mammals. This variation raises the fundamental question of how spinal circuits have changed in register with motor behavior. In simple, undulatory fish, exemplified by the lamprey, two broad classes of interneurons shape motor neuron output: ipsilateral-projecting excitatory neurons, and commissural-projecting inhibitory neurons. An additional class of ipsilateral inhibitory neurons is required to generate escape swim behavior in larval zebrafish and tadpoles. In limbed vertebrates, a more complex spinal neuron composition is observed. In this review, we provide evidence that movement elaboration correlates with an increase and specialization of these three basic interneuron types into molecularly, anatomically, and functionally distinct subpopulations. We summarize recent work linking neuron types to movement-pattern generation across fish, amphibians, reptiles, birds and mammals.","lang":"eng"}],"doi":"10.3389/fncir.2023.1146449","article_processing_charge":"Yes","publication":"Frontiers in Neural Circuits","date_published":"2023-04-26T00:00:00Z","acknowledgement":"This work was supported by the ERC Starting grant, ERC-2021-STG #101041551.","year":"2023","intvolume":"        17","article_type":"original","date_updated":"2026-04-07T12:36:07Z","citation":{"ista":"Wilson AC, Sweeney LB. 2023. Spinal cords: Symphonies of interneurons across species. Frontiers in Neural Circuits. 17, 1146449.","short":"A.C. Wilson, L.B. Sweeney, Frontiers in Neural Circuits 17 (2023).","ieee":"A. C. Wilson and L. B. Sweeney, “Spinal cords: Symphonies of interneurons across species,” <i>Frontiers in Neural Circuits</i>, vol. 17. Frontiers, 2023.","apa":"Wilson, A. C., &#38; Sweeney, L. B. (2023). Spinal cords: Symphonies of interneurons across species. <i>Frontiers in Neural Circuits</i>. Frontiers. <a href=\"https://doi.org/10.3389/fncir.2023.1146449\">https://doi.org/10.3389/fncir.2023.1146449</a>","ama":"Wilson AC, Sweeney LB. Spinal cords: Symphonies of interneurons across species. <i>Frontiers in Neural Circuits</i>. 2023;17. doi:<a href=\"https://doi.org/10.3389/fncir.2023.1146449\">10.3389/fncir.2023.1146449</a>","mla":"Wilson, Alexia C., and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” <i>Frontiers in Neural Circuits</i>, vol. 17, 1146449, Frontiers, 2023, doi:<a href=\"https://doi.org/10.3389/fncir.2023.1146449\">10.3389/fncir.2023.1146449</a>.","chicago":"Wilson, Alexia C, and Lora B. Sweeney. “Spinal Cords: Symphonies of Interneurons across Species.” <i>Frontiers in Neural Circuits</i>. Frontiers, 2023. <a href=\"https://doi.org/10.3389/fncir.2023.1146449\">https://doi.org/10.3389/fncir.2023.1146449</a>."},"file_date_updated":"2024-01-03T13:33:21Z","_id":"13097","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"type":"journal_article","month":"04","title":"Spinal cords: Symphonies of interneurons across species","status":"public","publisher":"Frontiers","department":[{"_id":"LoSw"}],"related_material":{"record":[{"id":"20735","relation":"dissertation_contains","status":"public"}]},"quality_controlled":"1"}]