---
OA_place: publisher
_id: '20212'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "I would also like to\r\nthank the Austrian Academy of Sciences for
  awarding me a 2-year DOC fellowship\r\n(DOC26253)."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Osvaldo
  full_name: Miranda, Osvaldo
  id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
  last_name: Miranda
  orcid: 0000-0001-6618-6889
citation:
  ama: Miranda O. Unraveling the role of Pten in cortical stem cell lineage progression
    using MADM. 2025. doi:<a href="https://doi.org/10.15479/AT-ISTA-20212">10.15479/AT-ISTA-20212</a>
  apa: Miranda, O. (2025). <i>Unraveling the role of Pten in cortical stem cell lineage
    progression using MADM</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT-ISTA-20212">https://doi.org/10.15479/AT-ISTA-20212</a>
  chicago: Miranda, Osvaldo. “Unraveling the Role of Pten in Cortical Stem Cell Lineage
    Progression Using MADM.” Institute of Science and Technology Austria, 2025. <a
    href="https://doi.org/10.15479/AT-ISTA-20212">https://doi.org/10.15479/AT-ISTA-20212</a>.
  ieee: O. Miranda, “Unraveling the role of Pten in cortical stem cell lineage progression
    using MADM,” Institute of Science and Technology Austria, 2025.
  ista: Miranda O. 2025. Unraveling the role of Pten in cortical stem cell lineage
    progression using MADM. Institute of Science and Technology Austria.
  mla: Miranda, Osvaldo. <i>Unraveling the Role of Pten in Cortical Stem Cell Lineage
    Progression Using MADM</i>. Institute of Science and Technology Austria, 2025,
    doi:<a href="https://doi.org/10.15479/AT-ISTA-20212">10.15479/AT-ISTA-20212</a>.
  short: O. Miranda, Unraveling the Role of Pten in Cortical Stem Cell Lineage Progression
    Using MADM, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-08-22T14:07:00Z
date_published: 2025-08-22T00:00:00Z
date_updated: 2026-04-14T08:16:57Z
day: '22'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SiHi
doi: 10.15479/AT-ISTA-20212
file:
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  date_updated: 2025-08-26T09:03:50Z
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  creator: omiranda
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  file_size: 28636240
  relation: main_file
file_date_updated: 2025-08-26T10:43:30Z
has_accepted_license: '1'
keyword:
- Pten
- mtor
- cortical development
- MADM
- Mapk
language:
- iso: eng
month: '08'
oa_version: Published Version
page: '119'
project:
- _id: 34c9fbcb-11ca-11ed-8bc3-98fa5658610d
  grant_number: '26253'
  name: Molecular Mechanisms Regulating Cortical Neural Stem Cell Lineage Progression
    and Astrocyte Development
publication_identifier:
  isbn:
  - 978-3-99078-063-3
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '17425'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
title: Unraveling the role of Pten in cortical stem cell lineage progression using
  MADM
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
OA_place: publisher
_id: '20737'
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "I also want to thank ISTA and the Austrian Science Fund FWF SFB
  F78 (F7805) for financially\r\nsupporting my research."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Raquel
  full_name: Casado Polanco, Raquel
  id: 15240fc1-dbcd-11ea-9d1d-ac5a786425fd
  last_name: Casado Polanco
  orcid: 0000-0001-8293-4568
citation:
  ama: Casado Polanco R. Role of NOTCH signaling in radial glial progenitor lineage
    progression. 2025. doi:<a href="https://doi.org/10.15479/AT-ISTA-20737">10.15479/AT-ISTA-20737</a>
  apa: Casado Polanco, R. (2025). <i>Role of NOTCH signaling in radial glial progenitor
    lineage progression</i>. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT-ISTA-20737">https://doi.org/10.15479/AT-ISTA-20737</a>
  chicago: Casado Polanco, Raquel. “Role of NOTCH Signaling in Radial Glial Progenitor
    Lineage Progression.” Institute of Science and Technology Austria, 2025. <a href="https://doi.org/10.15479/AT-ISTA-20737">https://doi.org/10.15479/AT-ISTA-20737</a>.
  ieee: R. Casado Polanco, “Role of NOTCH signaling in radial glial progenitor lineage
    progression,” Institute of Science and Technology Austria, 2025.
  ista: Casado Polanco R. 2025. Role of NOTCH signaling in radial glial progenitor
    lineage progression. Institute of Science and Technology Austria.
  mla: Casado Polanco, Raquel. <i>Role of NOTCH Signaling in Radial Glial Progenitor
    Lineage Progression</i>. Institute of Science and Technology Austria, 2025, doi:<a
    href="https://doi.org/10.15479/AT-ISTA-20737">10.15479/AT-ISTA-20737</a>.
  short: R. Casado Polanco, Role of NOTCH Signaling in Radial Glial Progenitor Lineage
    Progression, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-12-09T09:04:18Z
date_published: 2025-12-09T00:00:00Z
date_updated: 2026-04-14T08:16:58Z
day: '09'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SiHi
doi: 10.15479/AT-ISTA-20737
file:
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  date_updated: 2025-12-11T11:18:37Z
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  relation: source_file
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  creator: rcasadop
  date_created: 2025-12-11T09:28:04Z
  date_updated: 2025-12-11T09:28:04Z
  embargo: 2026-12-01
  embargo_to: open_access
  file_id: '20794'
  file_name: 2025_CasadoPolanco_Raquel_Thesis.pdf
  file_size: 6261874
  relation: main_file
file_date_updated: 2025-12-11T11:18:37Z
has_accepted_license: '1'
keyword:
- NOTCH
- radial glial progenitor
- lineage progression
- cortical development
language:
- iso: eng
month: '12'
oa_version: Published Version
page: '133'
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F7805
  name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
    Mechanisms of Neural Stem Cell Lineage Progression
publication_identifier:
  isbn:
  - 978-3-99078-072-5
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
status: public
supervisor:
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
title: Role of NOTCH signaling in radial glial progenitor lineage progression
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2025'
...
---
OA_place: publisher
_id: '9962'
abstract:
- lang: eng
  text: The brain is one of the largest and most complex organs and it is composed
    of billions of neurons that communicate together enabling e.g. consciousness.
    The cerebral cortex is the largest site of neural integration in the central nervous
    system. Concerted radial migration of newly born cortical projection neurons,
    from their birthplace to their final position, is a key step in the assembly of
    the cerebral cortex. The cellular and molecular mechanisms regulating radial neuronal
    migration in vivo are however still unclear. Recent evidence suggests that distinct
    signaling cues act cell-autonomously but differentially at certain steps during
    the overall migration process. Moreover, functional analysis of genetic mosaics
    (mutant neurons present in wild-type/heterozygote environment) using the MADM
    (Mosaic Analysis with Double Markers) analyses in comparison to global knockout
    also indicate a significant degree of non-cell-autonomous and/or community effects
    in the control of cortical neuron migration. The interactions of cell-intrinsic
    (cell-autonomous) and cell-extrinsic (non-cell-autonomous) components are largely
    unknown. In part of this thesis work we established a MADM-based experimental
    strategy for the quantitative analysis of cell-autonomous gene function versus
    non-cell-autonomous and/or community effects. The direct comparison of mutant
    neurons from the genetic mosaic (cell-autonomous) to mutant neurons in the conditional
    and/or global knockout (cell-autonomous + non-cell-autonomous) allows to quantitatively
    analyze non-cell-autonomous effects. Such analysis enable the high-resolution
    analysis of projection neuron migration dynamics in distinct environments with
    concomitant isolation of genomic and proteomic profiles. Using these experimental
    paradigms and in combination with computational modeling we show and characterize
    the nature of non-cell-autonomous effects to coordinate radial neuron migration.
    Furthermore, this thesis discusses recent developments in neurodevelopment with
    focus on neuronal polarization and non-cell-autonomous mechanisms in neuronal
    migration.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Andi H
  full_name: Hansen, Andi H
  id: 38853E16-F248-11E8-B48F-1D18A9856A87
  last_name: Hansen
citation:
  ama: Hansen AH. Cell-autonomous gene function and non-cell-autonomous effects in
    radial projection neuron migration. 2021. doi:<a href="https://doi.org/10.15479/at:ista:9962">10.15479/at:ista:9962</a>
  apa: Hansen, A. H. (2021). <i>Cell-autonomous gene function and non-cell-autonomous
    effects in radial projection neuron migration</i>. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/at:ista:9962">https://doi.org/10.15479/at:ista:9962</a>
  chicago: Hansen, Andi H. “Cell-Autonomous Gene Function and Non-Cell-Autonomous
    Effects in Radial Projection Neuron Migration.” Institute of Science and Technology
    Austria, 2021. <a href="https://doi.org/10.15479/at:ista:9962">https://doi.org/10.15479/at:ista:9962</a>.
  ieee: A. H. Hansen, “Cell-autonomous gene function and non-cell-autonomous effects
    in radial projection neuron migration,” Institute of Science and Technology Austria,
    2021.
  ista: Hansen AH. 2021. Cell-autonomous gene function and non-cell-autonomous effects
    in radial projection neuron migration. Institute of Science and Technology Austria.
  mla: Hansen, Andi H. <i>Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects
    in Radial Projection Neuron Migration</i>. Institute of Science and Technology
    Austria, 2021, doi:<a href="https://doi.org/10.15479/at:ista:9962">10.15479/at:ista:9962</a>.
  short: A.H. Hansen, Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects
    in Radial Projection Neuron Migration, Institute of Science and Technology Austria,
    2021.
corr_author: '1'
date_created: 2021-08-29T12:36:50Z
date_published: 2021-09-02T00:00:00Z
date_updated: 2026-04-08T07:19:09Z
day: '02'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: SiHi
doi: 10.15479/at:ista:9962
file:
- access_level: closed
  checksum: 66b56f5b988b233dc66a4f4b4fb2cdfe
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: ahansen
  date_created: 2021-08-30T09:17:39Z
  date_updated: 2022-09-03T22:30:04Z
  embargo_to: open_access
  file_id: '9971'
  file_name: Thesis_Hansen.docx
  file_size: 10629190
  relation: source_file
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  checksum: 204fa40321a1c6289b68c473634c4bf3
  content_type: application/pdf
  creator: ahansen
  date_created: 2021-08-30T09:29:44Z
  date_updated: 2022-09-03T22:30:04Z
  embargo: 2022-09-02
  file_id: '9972'
  file_name: Thesis_Hansen_PDFA-1a.pdf
  file_size: 13457469
  relation: main_file
file_date_updated: 2022-09-03T22:30:04Z
has_accepted_license: '1'
keyword:
- Neuronal migration
- Non-cell-autonomous
- Cell-autonomous
- Neurodevelopmental disease
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: '182'
project:
- _id: 2625A13E-B435-11E9-9278-68D0E5697425
  grant_number: '24812'
  name: Molecular mechanisms of radial neuronal migration
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '8569'
    relation: part_of_dissertation
    status: public
  - id: '960'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
title: Cell-autonomous gene function and non-cell-autonomous effects in radial projection
  neuron migration
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2021'
...
---
OA_place: publisher
_id: '7902'
abstract:
- lang: eng
  text: "Mosaic genetic analysis has been widely used in different model organisms
    such as the fruit fly to study gene-function in a cell-autonomous or tissue-specific
    fashion. More recently, and less easily conducted, mosaic genetic analysis in
    mice has also been enabled with the ambition to shed light on human gene function
    and disease. These genetic tools are of particular interest, but not restricted
    to, the study of the brain. Notably, the MADM technology offers a genetic approach
    in mice to visualize and concomitantly manipulate small subsets of genetically
    defined cells at a clonal level and single cell resolution. MADM-based analysis
    has already advanced the study of genetic mechanisms regulating brain development
    and is expected that further MADM-based analysis of genetic alterations will continue
    to reveal important insights on the fundamental principles of development and
    disease to potentially assist in the development of new therapies or treatments.\r\nIn
    summary, this work completed and characterized the necessary genome-wide genetic
    tools to perform MADM-based analysis at single cell level of the vast majority
    of mouse genes in virtually any cell type and provided a protocol to perform lineage
    tracing using the novel MADM resource. Importantly, this work also explored and
    revealed novel aspects of biologically relevant events in an in vivo context,
    such as the chromosome-specific bias of chromatid sister segregation pattern,
    the generation of cell-type diversity in the cerebral cortex and in the cerebellum
    and finally, the relevance of the interplay between the cell-autonomous gene function
    and cell-non-autonomous (community) effects in radial glial progenitor lineage
    progression.\r\nThis work provides a foundation and opens the door to further
    elucidating the molecular mechanisms underlying neuronal diversity and astrocyte
    generation."
acknowledged_ssus:
- _id: PreCl
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Ximena
  full_name: Contreras, Ximena
  id: 475990FE-F248-11E8-B48F-1D18A9856A87
  last_name: Contreras
citation:
  ama: Contreras X. Genetic dissection of neural development in health and disease
    at single cell resolution. 2020. doi:<a href="https://doi.org/10.15479/AT:ISTA:7902">10.15479/AT:ISTA:7902</a>
  apa: Contreras, X. (2020). <i>Genetic dissection of neural development in health
    and disease at single cell resolution</i>. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/AT:ISTA:7902">https://doi.org/10.15479/AT:ISTA:7902</a>
  chicago: Contreras, Ximena. “Genetic Dissection of Neural Development in Health
    and Disease at Single Cell Resolution.” Institute of Science and Technology Austria,
    2020. <a href="https://doi.org/10.15479/AT:ISTA:7902">https://doi.org/10.15479/AT:ISTA:7902</a>.
  ieee: X. Contreras, “Genetic dissection of neural development in health and disease
    at single cell resolution,” Institute of Science and Technology Austria, 2020.
  ista: Contreras X. 2020. Genetic dissection of neural development in health and
    disease at single cell resolution. Institute of Science and Technology Austria.
  mla: Contreras, Ximena. <i>Genetic Dissection of Neural Development in Health and
    Disease at Single Cell Resolution</i>. Institute of Science and Technology Austria,
    2020, doi:<a href="https://doi.org/10.15479/AT:ISTA:7902">10.15479/AT:ISTA:7902</a>.
  short: X. Contreras, Genetic Dissection of Neural Development in Health and Disease
    at Single Cell Resolution, Institute of Science and Technology Austria, 2020.
corr_author: '1'
date_created: 2020-05-29T08:27:32Z
date_published: 2020-06-05T00:00:00Z
date_updated: 2026-04-16T09:52:49Z
day: '05'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: SiHi
doi: 10.15479/AT:ISTA:7902
ec_funded: 1
file:
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  date_updated: 2021-06-07T22:30:03Z
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  creator: xcontreras
  date_created: 2020-06-05T08:18:07Z
  date_updated: 2021-06-07T22:30:03Z
  embargo: 2021-06-06
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file_date_updated: 2021-06-07T22:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '214'
project:
- _id: 260018B0-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '725780'
  name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
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    relation: dissertation_contains
    status: public
  - id: '7815'
    relation: dissertation_contains
    status: public
  - id: '6830'
    relation: dissertation_contains
    status: public
status: public
supervisor:
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
title: Genetic dissection of neural development in health and disease at single cell
  resolution
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2020'
...