---
_id: '11126'
abstract:
- lang: eng
text: Nuclear import of the two uracil-rich small nuclear ribonucleoprotein (U snRNP)
components U1A and U2B′′ is mediated by unusually long and complex nuclear localization
signals (NLSs). Here we investigate nuclear import of U1A and U2B′′ in vitro and
demonstrate that it occurs by an active, saturable process. Several lines of evidence
suggest that import of the two proteins occurs by an import mechanism different
to those characterized previously. No cross competition is seen with a variety
of previously studied NLSs. In contrast to import mediated by members of the importin-β
family of nucleocytoplasmic transport receptors, U1A/U2B′′ import is not inhibited
by either nonhydrolyzable guanosine triphosphate (GTP) analogues or by a mutant
of the GTPase Ran that is incapable of GTP hydrolysis. Adenosine triphosphate
is capable of supporting U1A and U2B′′ import, whereas neither nonhydrolyzable
adenosine triphosphate analogues nor GTP can do so. U1A and U2B′′ import in vitro
does not require the addition of soluble cytosolic proteins, but a factor or factors
required for U1A and U2B′′ import remains tightly associated with the nuclear
fraction of conventionally permeabilized cells. This activity can be solubilized
in the presence of elevated MgCl2. These data suggest that U1A and U2B′′ import
into the nucleus occurs by a hitherto uncharacterized mechanism.
article_processing_charge: No
article_type: original
author:
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
- first_name: Iain W.
full_name: Mattaj, Iain W.
last_name: Mattaj
citation:
ama: Hetzer M, Mattaj IW. An Atp-dependent, Ran-independent mechanism for nuclear
import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology.
2000;148(2):293-304. doi:10.1083/jcb.148.2.293
apa: Hetzer, M., & Mattaj, I. W. (2000). An Atp-dependent, Ran-independent mechanism
for nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell
Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.148.2.293
chicago: Hetzer, Martin, and Iain W. Mattaj. “An Atp-Dependent, Ran-Independent
Mechanism for Nuclear Import of the U1a and U2b′′ Spliceosome Proteins.” Journal
of Cell Biology. Rockefeller University Press, 2000. https://doi.org/10.1083/jcb.148.2.293.
ieee: M. Hetzer and I. W. Mattaj, “An Atp-dependent, Ran-independent mechanism for
nuclear import of the U1a and U2b′′ spliceosome proteins,” Journal of Cell
Biology, vol. 148, no. 2. Rockefeller University Press, pp. 293–304, 2000.
ista: Hetzer M, Mattaj IW. 2000. An Atp-dependent, Ran-independent mechanism for
nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology.
148(2), 293–304.
mla: Hetzer, Martin, and Iain W. Mattaj. “An Atp-Dependent, Ran-Independent Mechanism
for Nuclear Import of the U1a and U2b′′ Spliceosome Proteins.” Journal of Cell
Biology, vol. 148, no. 2, Rockefeller University Press, 2000, pp. 293–304,
doi:10.1083/jcb.148.2.293.
short: M. Hetzer, I.W. Mattaj, Journal of Cell Biology 148 (2000) 293–304.
date_created: 2022-04-07T07:57:49Z
date_published: 2000-01-24T00:00:00Z
date_updated: 2022-07-18T08:58:29Z
day: '24'
doi: 10.1083/jcb.148.2.293
extern: '1'
external_id:
pmid:
- '10648562'
fulldoi: https://doi.org/10.1083/jcb.148.2.293
intvolume: ' 148'
issue: '2'
keyword:
- Cell Biology
language:
- iso: eng
month: '01'
oa_version: None
page: 293-304
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
issn:
- 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and
U2b′′ spliceosome proteins
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 148
year: '2000'
...
---
_id: '11127'
abstract:
- lang: eng
text: Nuclear formation in Xenopus egg extracts requires cytosol and is inhibited
by GTPγS, indicating a requirement for GTPase activity. Nuclear envelope (NE)
vesicle fusion is extensively inhibited by GTPγS and two mutant forms of the Ran
GTPase, Q69L and T24N. Depletion of either Ran or RCC1, the exchange factor for
Ran, from the assembly reaction also inhibits this step of NE formation. Ran depletion
can be complemented by the addition of Ran loaded with either GTP or GDP but not
with GTPγS. RCC1 depletion is only complemented by RCC1 itself or by RanGTP. Thus,
generation of RanGTP by RCC1 and GTP hydrolysis by Ran are both required for the
extensive membrane fusion events that lead to NE formation.
article_processing_charge: No
article_type: original
author:
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
- first_name: Daniel
full_name: Bilbao-Cortés, Daniel
last_name: Bilbao-Cortés
- first_name: Tobias C
full_name: Walther, Tobias C
last_name: Walther
- first_name: Oliver J
full_name: Gruss, Oliver J
last_name: Gruss
- first_name: Iain W
full_name: Mattaj, Iain W
last_name: Mattaj
citation:
ama: Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. GTP hydrolysis
by Ran is required for nuclear envelope assembly. Molecular Cell. 2000;5(6):1013-1024.
doi:10.1016/s1097-2765(00)80266-x
apa: Hetzer, M., Bilbao-Cortés, D., Walther, T. C., Gruss, O. J., & Mattaj,
I. W. (2000). GTP hydrolysis by Ran is required for nuclear envelope assembly.
Molecular Cell. Elsevier. https://doi.org/10.1016/s1097-2765(00)80266-x
chicago: Hetzer, Martin, Daniel Bilbao-Cortés, Tobias C Walther, Oliver J Gruss,
and Iain W Mattaj. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly.”
Molecular Cell. Elsevier, 2000. https://doi.org/10.1016/s1097-2765(00)80266-x.
ieee: M. Hetzer, D. Bilbao-Cortés, T. C. Walther, O. J. Gruss, and I. W. Mattaj,
“GTP hydrolysis by Ran is required for nuclear envelope assembly,” Molecular
Cell, vol. 5, no. 6. Elsevier, pp. 1013–1024, 2000.
ista: Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. 2000. GTP hydrolysis
by Ran is required for nuclear envelope assembly. Molecular Cell. 5(6), 1013–1024.
mla: Hetzer, Martin, et al. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope
Assembly.” Molecular Cell, vol. 5, no. 6, Elsevier, 2000, pp. 1013–24,
doi:10.1016/s1097-2765(00)80266-x.
short: M. Hetzer, D. Bilbao-Cortés, T.C. Walther, O.J. Gruss, I.W. Mattaj, Molecular
Cell 5 (2000) 1013–1024.
date_created: 2022-04-07T07:57:59Z
date_published: 2000-06-01T00:00:00Z
date_updated: 2022-07-18T08:58:31Z
day: '01'
doi: 10.1016/s1097-2765(00)80266-x
extern: '1'
external_id:
pmid:
- '10911995'
fulldoi: https://doi.org/10.1016/s1097-2765(00)80266-x
intvolume: ' 5'
issue: '6'
keyword:
- Cell Biology
- Molecular Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/S1097-2765(00)80266-X
month: '06'
oa: 1
oa_version: Published Version
page: 1013-1024
pmid: 1
publication: Molecular Cell
publication_identifier:
issn:
- 1097-2765
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: GTP hydrolysis by Ran is required for nuclear envelope assembly
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 5
year: '2000'
...
---
_id: '11683'
abstract:
- lang: eng
text: The vertex connectivity κ of a graph is the smallest number of vertices whose
deletion separates the graph or makes it trivial. We present the fastest known
deterministic algorithm for finding the vertex connectivity and a corresponding
separator. The time for a digraph having n vertices and m edges is O(min{κ3 +
n, κn}m); for an undirected graph the term m can be replaced by κn. A randomized
algorithm finds κ with error probability 1/2 in time O(nm). If the vertices have
nonnegative weights the weighted vertex connectivity is found in time O(κ1nmlog(n2/m))
where κ1 ≤ m/n is the unweighted vertex connectivity or in expected time O(nmlog(n2/m))
with error probability 1/2. The main algorithm combines two previous vertex connectivity
algorithms and a generalization of the preflow-push algorithm of Hao and Orlin
(1994, J. Algorithms17, 424–446) that computes edge connectivity.
article_processing_charge: No
article_type: original
author:
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
- first_name: Satish
full_name: Rao, Satish
last_name: Rao
- first_name: Harold N.
full_name: Gabow, Harold N.
last_name: Gabow
citation:
ama: 'Henzinger M, Rao S, Gabow HN. Computing vertex connectivity: New bounds from
old techniques. Journal of Algorithms. 2000;34(2):222-250. doi:10.1006/jagm.1999.1055'
apa: 'Henzinger, M., Rao, S., & Gabow, H. N. (2000). Computing vertex connectivity:
New bounds from old techniques. Journal of Algorithms. Elsevier. https://doi.org/10.1006/jagm.1999.1055'
chicago: 'Henzinger, Monika, Satish Rao, and Harold N. Gabow. “Computing Vertex
Connectivity: New Bounds from Old Techniques.” Journal of Algorithms. Elsevier,
2000. https://doi.org/10.1006/jagm.1999.1055.'
ieee: 'M. Henzinger, S. Rao, and H. N. Gabow, “Computing vertex connectivity: New
bounds from old techniques,” Journal of Algorithms, vol. 34, no. 2. Elsevier,
pp. 222–250, 2000.'
ista: 'Henzinger M, Rao S, Gabow HN. 2000. Computing vertex connectivity: New bounds
from old techniques. Journal of Algorithms. 34(2), 222–250.'
mla: 'Henzinger, Monika, et al. “Computing Vertex Connectivity: New Bounds from
Old Techniques.” Journal of Algorithms, vol. 34, no. 2, Elsevier, 2000,
pp. 222–50, doi:10.1006/jagm.1999.1055.'
short: M. Henzinger, S. Rao, H.N. Gabow, Journal of Algorithms 34 (2000) 222–250.
date_created: 2022-07-28T08:56:10Z
date_published: 2000-02-01T00:00:00Z
date_updated: 2024-11-04T11:42:08Z
day: '01'
doi: 10.1006/jagm.1999.1055
extern: '1'
fulldoi: https://doi.org/10.1006/jagm.1999.1055
intvolume: ' 34'
issue: '2'
keyword:
- Computational Theory and Mathematics
- Computational Mathematics
- Control and Optimization
language:
- iso: eng
month: '02'
oa_version: None
page: 222-250
publication: Journal of Algorithms
publication_identifier:
issn:
- 0196-6774
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Computing vertex connectivity: New bounds from old techniques'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 34
year: '2000'
...
---
_id: '11685'
abstract:
- lang: eng
text: We consider the problem of sampling URLs uniformly at random from the Web.
A tool for sampling URLs uniformly can be used to estimate various properties
of Web pages, such as the fraction of pages in various Internet domains or written
in various languages. Moreover, uniform URL sampling can be used to determine
the sizes of various search engines relative to the entire Web. In this paper,
we consider sampling approaches based on random walks of the Web graph. In particular,
we suggest ways of improving sampling based on random walks to make the samples
closer to uniform. We suggest a natural test bed based on random graphs for testing
the effectiveness of our procedures. We then use our sampling approach to estimate
the distribution of pages over various Internet domains and to estimate the coverage
of various search engine indexes.
article_processing_charge: No
article_type: original
author:
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
- first_name: Allan
full_name: Heydon, Allan
last_name: Heydon
- first_name: Michael
full_name: Mitzenmacher, Michael
last_name: Mitzenmacher
- first_name: Marc
full_name: Najork, Marc
last_name: Najork
citation:
ama: Henzinger M, Heydon A, Mitzenmacher M, Najork M. On near-uniform URL sampling.
Computer Networks. 2000;33(1-6):295-308. doi:10.1016/s1389-1286(00)00055-4
apa: Henzinger, M., Heydon, A., Mitzenmacher, M., & Najork, M. (2000). On near-uniform
URL sampling. Computer Networks. Elsevier. https://doi.org/10.1016/s1389-1286(00)00055-4
chicago: Henzinger, Monika, Allan Heydon, Michael Mitzenmacher, and Marc Najork.
“On Near-Uniform URL Sampling.” Computer Networks. Elsevier, 2000. https://doi.org/10.1016/s1389-1286(00)00055-4.
ieee: M. Henzinger, A. Heydon, M. Mitzenmacher, and M. Najork, “On near-uniform
URL sampling,” Computer Networks, vol. 33, no. 1–6. Elsevier, pp. 295–308,
2000.
ista: Henzinger M, Heydon A, Mitzenmacher M, Najork M. 2000. On near-uniform URL
sampling. Computer Networks. 33(1–6), 295–308.
mla: Henzinger, Monika, et al. “On Near-Uniform URL Sampling.” Computer Networks,
vol. 33, no. 1–6, Elsevier, 2000, pp. 295–308, doi:10.1016/s1389-1286(00)00055-4.
short: M. Henzinger, A. Heydon, M. Mitzenmacher, M. Najork, Computer Networks 33
(2000) 295–308.
date_created: 2022-07-28T15:11:53Z
date_published: 2000-06-01T00:00:00Z
date_updated: 2024-11-06T08:11:55Z
day: '01'
doi: 10.1016/s1389-1286(00)00055-4
extern: '1'
fulldoi: https://doi.org/10.1016/s1389-1286(00)00055-4
intvolume: ' 33'
issue: 1-6
keyword:
- URL sampling
- Random walks
- Internet domain distribution
- Search engine size
language:
- iso: eng
month: '06'
oa_version: None
page: 295-308
publication: Computer Networks
publication_identifier:
issn:
- 1389-1286
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: On near-uniform URL sampling
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 33
year: '2000'
...
---
_id: '11694'
abstract:
- lang: eng
text: We consider exploration problems where a robot has to construct a complete
map of an unknown environment. We assume that the environment is modeled by a
directed, strongly connected graph. The robot's task is to visit all nodes and
edges of the graph using the minimum number R of edge traversals. Deng and Papadimitriou
[Proceedings of the 31st Symposium on the Foundations of Computer Science, 1990,
pp. 356-361] showed an upper bound for R ofd O(d)m and Koutsoupias (reported by
Deng and Papadimitriou) gave a lower bound of Ω≠(d2m), where m is the number of
edges in the graph and d is the minimum number of edges that have to be added
to make the graph Eulerian. We give the 1rst subexponential algorithm for this
exploration problem, which achieves an upper bound of dO(logd)m. We also show
a matching lower bound of d≠(logd)m for our algorithm. Additionally, we give lower
bounds of 2≠(d)m, respectively, d≠(logd)m for various other natural exploration
algorithms.
acknowledgement: We thank Prabhakar Raghavan for bringing to our attention the literature
on the s-t connectivity problem. We also thank an anonymous referee for many helpful
comments which improved the presentation of the paper.
article_processing_charge: No
article_type: original
author:
- first_name: Susanne
full_name: Albers, Susanne
last_name: Albers
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
citation:
ama: Albers S, Henzinger M. Exploring unknown environments. SIAM Journal on Computing.
2000;29(4):1164-1188. doi:10.1137/s009753979732428x
apa: 'Albers, S., & Henzinger, M. (2000). Exploring unknown environments. SIAM
Journal on Computing. El Paso, TX, United States: Society for Industrial and
Applied Mathematics. https://doi.org/10.1137/s009753979732428x'
chicago: Albers, Susanne, and Monika Henzinger. “Exploring Unknown Environments.”
SIAM Journal on Computing. Society for Industrial and Applied Mathematics,
2000. https://doi.org/10.1137/s009753979732428x.
ieee: S. Albers and M. Henzinger, “Exploring unknown environments,” SIAM Journal
on Computing, vol. 29, no. 4. Society for Industrial and Applied Mathematics,
pp. 1164–1188, 2000.
ista: Albers S, Henzinger M. 2000. Exploring unknown environments. SIAM Journal
on Computing. 29(4), 1164–1188.
mla: Albers, Susanne, and Monika Henzinger. “Exploring Unknown Environments.” SIAM
Journal on Computing, vol. 29, no. 4, Society for Industrial and Applied Mathematics,
2000, pp. 1164–88, doi:10.1137/s009753979732428x.
short: S. Albers, M. Henzinger, SIAM Journal on Computing 29 (2000) 1164–1188.
conference:
end_date: 1997-05-06
location: El Paso, TX, United States
name: 'STOC97: 29th Annual Symposium on Theory of Computing'
start_date: 1997-05-04
date_created: 2022-07-29T09:04:36Z
date_published: 2000-07-01T00:00:00Z
date_updated: 2024-11-06T12:09:07Z
day: '01'
doi: 10.1137/s009753979732428x
extern: '1'
fulldoi: https://doi.org/10.1137/s009753979732428x
intvolume: ' 29'
issue: '4'
keyword:
- directed graph
- exploration algorithm
language:
- iso: eng
month: '07'
oa_version: None
page: 1164-1188
publication: SIAM Journal on Computing
publication_identifier:
eissn:
- 1095-7111
issn:
- 0097-5397
publication_status: published
publisher: Society for Industrial and Applied Mathematics
quality_controlled: '1'
scopus_import: '1'
status: public
title: Exploring unknown environments
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2000'
...
---
_id: '11770'
abstract:
- lang: eng
text: 'We compare several algorithms for identifying mirrored hosts on the World
Wide Web. The algorithms operate on the basis of URL strings and linkage data:
the type of information about Web pages easily available from Web proxies and
crawlers. Identification of mirrored hosts can improve Web-based information retrieval
in several ways: first, by identifying mirrored hosts, search engines can avoid
storing and returning duplicate documents. Second, several new information retrieval
techniques for the Web make inferences based on the explicit links among hypertext
documents—mirroring perturbs their graph model and degrades performance. Third,
mirroring information can be used to redirect users to alternate mirror sites
to compensate for various failures, and can thus improve the performance of Web
browsers and proxies. We evaluated four classes of “top-down” algorithms for detecting
mirrored host pairs (that is, algorithms that are based on page attributes such
as URL, IP address, and hyperlinks between pages, and not on the page content)
on a collection of 140 million URLs (on 230,000 hosts) and their associated connectivity
information. Our best approach is one which combines five algorithms and achieved
a precision of 0.57 for a recall of 0.86 considering 100,000 ranked host pairs.'
article_processing_charge: No
article_type: original
author:
- first_name: Krishna
full_name: Bharat, Krishna
last_name: Bharat
- first_name: Andrei
full_name: Broder, Andrei
last_name: Broder
- first_name: Jeffrey
full_name: Dean, Jeffrey
last_name: Dean
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
citation:
ama: Bharat K, Broder A, Dean J, Henzinger M. A comparison of techniques to find
mirrored hosts on the WWW. Journal of the American Society for Information
Science. 2000;51(12):1114-1122. doi:10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0
apa: Bharat, K., Broder, A., Dean, J., & Henzinger, M. (2000). A comparison
of techniques to find mirrored hosts on the WWW. Journal of the American Society
for Information Science. Wiley. https://doi.org/10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0
chicago: Bharat, Krishna, Andrei Broder, Jeffrey Dean, and Monika Henzinger. “A
Comparison of Techniques to Find Mirrored Hosts on the WWW.” Journal of the
American Society for Information Science. Wiley, 2000. https://doi.org/10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0.
ieee: K. Bharat, A. Broder, J. Dean, and M. Henzinger, “A comparison of techniques
to find mirrored hosts on the WWW,” Journal of the American Society for Information
Science, vol. 51, no. 12. Wiley, pp. 1114–1122, 2000.
ista: Bharat K, Broder A, Dean J, Henzinger M. 2000. A comparison of techniques
to find mirrored hosts on the WWW. Journal of the American Society for Information
Science. 51(12), 1114–1122.
mla: Bharat, Krishna, et al. “A Comparison of Techniques to Find Mirrored Hosts
on the WWW.” Journal of the American Society for Information Science, vol.
51, no. 12, Wiley, 2000, pp. 1114–22, doi:10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0.
short: K. Bharat, A. Broder, J. Dean, M. Henzinger, Journal of the American Society
for Information Science 51 (2000) 1114–1122.
date_created: 2022-08-08T12:57:37Z
date_published: 2000-10-01T00:00:00Z
date_updated: 2024-11-06T08:14:37Z
day: '01'
doi: 10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0
extern: '1'
fulldoi: https://doi.org/10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0
intvolume: ' 51'
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1002/1097-4571(2000)9999:9999<::aid-asi1025>3.0.co;2-0
month: '10'
oa: 1
oa_version: Published Version
page: 1114-1122
publication: Journal of the American Society for Information Science
publication_identifier:
issn:
- 0002-8231
- 1097-4571
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: A comparison of techniques to find mirrored hosts on the WWW
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 51
year: '2000'
...
---
_id: '11802'
abstract:
- lang: eng
text: In this paper we survey algorithmic aspects of Web information retrieval.
As an example, we discuss ranking of search engine results using connectivity
analysis.
alternative_title:
- LNCS
article_processing_charge: No
author:
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
citation:
ama: 'Henzinger M. Web information retrieval - an algorithmic perspective. In: 8th
Annual European Symposium on Algorithms. Vol 1879. Springer Nature; 2000:1–8.
doi:10.1007/3-540-45253-2_1'
apa: 'Henzinger, M. (2000). Web information retrieval - an algorithmic perspective.
In 8th Annual European Symposium on Algorithms (Vol. 1879, pp. 1–8). Saarbrücken,
Germany: Springer Nature. https://doi.org/10.1007/3-540-45253-2_1'
chicago: Henzinger, Monika. “Web Information Retrieval - an Algorithmic Perspective.”
In 8th Annual European Symposium on Algorithms, 1879:1–8. Springer Nature,
2000. https://doi.org/10.1007/3-540-45253-2_1.
ieee: M. Henzinger, “Web information retrieval - an algorithmic perspective,” in
8th Annual European Symposium on Algorithms, Saarbrücken, Germany, 2000,
vol. 1879, pp. 1–8.
ista: 'Henzinger M. 2000. Web information retrieval - an algorithmic perspective.
8th Annual European Symposium on Algorithms. ESA: European Symposium on Algorithms,
LNCS, vol. 1879, 1–8.'
mla: Henzinger, Monika. “Web Information Retrieval - an Algorithmic Perspective.”
8th Annual European Symposium on Algorithms, vol. 1879, Springer Nature,
2000, pp. 1–8, doi:10.1007/3-540-45253-2_1.
short: M. Henzinger, in:, 8th Annual European Symposium on Algorithms, Springer
Nature, 2000, pp. 1–8.
conference:
end_date: 2000-09-08
location: Saarbrücken, Germany
name: 'ESA: European Symposium on Algorithms'
start_date: 2000-09-05
date_created: 2022-08-11T13:25:07Z
date_published: 2000-09-01T00:00:00Z
date_updated: 2024-11-06T11:55:46Z
day: '01'
doi: 10.1007/3-540-45253-2_1
extern: '1'
fulldoi: https://doi.org/10.1007/3-540-45253-2_1
intvolume: ' 1879'
language:
- iso: eng
month: '09'
oa_version: None
page: 1–8
publication: 8th Annual European Symposium on Algorithms
publication_identifier:
eisbn:
- '9783540452539'
eissn:
- 1611-3349
isbn:
- '9783540410041'
issn:
- 0302-9743
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Web information retrieval - an algorithmic perspective
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1879
year: '2000'
...
---
_id: '11893'
abstract:
- lang: eng
text: "We present fully dynamic algorithms for maintaining the biconnected components
in general and plane graphs.\r\n\r\nA fully dynamic algorithm maintains a graph
during a sequence of insertions and deletions of edges or isolated vertices. Let
m be the number of edges and n be the number of vertices in a graph. The time
per operation of the best deterministic algorithms is \U0001D442(\U0001D45B√)
in general graphs and O(log n) in plane graphs for fully dynamic connectivity
and O(min m2/3 ,n}) in general graphs and \U0001D442(\U0001D45B√) in plane graphs
for fully dynamic biconnectivity. We improve the later running times to \U0001D442(\U0001D45Alog\U0001D45B‾‾‾‾‾‾‾√)
in general graphs and O(log 2n ) in plane graphs. Our algorithm for general graphscan
also find the biconnected components of all vertices in time O(n)."
article_processing_charge: No
article_type: original
author:
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
citation:
ama: Henzinger M. Improved data structures for fully dynamic biconnectivity. SIAM
Journal on Computing. 2000;29(6):1761-1815. doi:10.1137/s0097539794263907
apa: Henzinger, M. (2000). Improved data structures for fully dynamic biconnectivity.
SIAM Journal on Computing. Society for Industrial & Applied Mathematics.
https://doi.org/10.1137/s0097539794263907
chicago: Henzinger, Monika. “Improved Data Structures for Fully Dynamic Biconnectivity.”
SIAM Journal on Computing. Society for Industrial & Applied Mathematics,
2000. https://doi.org/10.1137/s0097539794263907.
ieee: M. Henzinger, “Improved data structures for fully dynamic biconnectivity,”
SIAM Journal on Computing, vol. 29, no. 6. Society for Industrial &
Applied Mathematics, pp. 1761–1815, 2000.
ista: Henzinger M. 2000. Improved data structures for fully dynamic biconnectivity.
SIAM Journal on Computing. 29(6), 1761–1815.
mla: Henzinger, Monika. “Improved Data Structures for Fully Dynamic Biconnectivity.”
SIAM Journal on Computing, vol. 29, no. 6, Society for Industrial &
Applied Mathematics, 2000, pp. 1761–815, doi:10.1137/s0097539794263907.
short: M. Henzinger, SIAM Journal on Computing 29 (2000) 1761–1815.
date_created: 2022-08-17T08:45:41Z
date_published: 2000-11-01T00:00:00Z
date_updated: 2024-11-06T12:00:56Z
day: '01'
doi: 10.1137/s0097539794263907
extern: '1'
fulldoi: https://doi.org/10.1137/s0097539794263907
intvolume: ' 29'
issue: '6'
language:
- iso: eng
month: '11'
oa_version: None
page: 1761-1815
publication: SIAM Journal on Computing
publication_identifier:
eissn:
- 1095-7111
issn:
- 0097-5397
publication_status: published
publisher: Society for Industrial & Applied Mathematics
quality_controlled: '1'
scopus_import: '1'
status: public
title: Improved data structures for fully dynamic biconnectivity
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2000'
...
---
_id: '2325'
alternative_title:
- Contemporary Mathematics
author:
- first_name: Robert
full_name: Robert Seiringer
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
citation:
ama: 'Seiringer R. Inequalities for Schrödinger operators and applications to the
stability of matter problem . In: Vol 529. American Mathematical Society; 2000:53-72.
doi:10.1090/conm/529'
apa: Seiringer, R. (2000). Inequalities for Schrödinger operators and applications
to the stability of matter problem (Vol. 529, pp. 53–72). Presented at the Arizona
School of Analysis With Applications, American Mathematical Society. https://doi.org/10.1090/conm/529
chicago: Seiringer, Robert. “Inequalities for Schrödinger Operators and Applications
to the Stability of Matter Problem ,” 529:53–72. American Mathematical Society,
2000. https://doi.org/10.1090/conm/529.
ieee: R. Seiringer, “Inequalities for Schrödinger operators and applications to
the stability of matter problem ,” presented at the Arizona School of Analysis
With Applications, 2000, vol. 529, pp. 53–72.
ista: Seiringer R. 2000. Inequalities for Schrödinger operators and applications
to the stability of matter problem . Arizona School of Analysis With Applications,
Contemporary Mathematics, vol. 529, 53–72.
mla: Seiringer, Robert. Inequalities for Schrödinger Operators and Applications
to the Stability of Matter Problem . Vol. 529, American Mathematical Society,
2000, pp. 53–72, doi:10.1090/conm/529.
short: R. Seiringer, in:, American Mathematical Society, 2000, pp. 53–72.
conference:
name: Arizona School of Analysis With Applications
date_created: 2018-12-11T11:57:00Z
date_published: 2000-01-01T00:00:00Z
date_updated: 2021-01-12T06:56:47Z
day: '01'
doi: 10.1090/conm/529
extern: 1
fulldoi: https://doi.org/10.1090/conm/529
intvolume: ' 529'
month: '01'
page: 53 - 72
publication_status: published
publisher: American Mathematical Society
publist_id: '4602'
quality_controlled: 0
status: public
title: 'Inequalities for Schrödinger operators and applications to the stability of
matter problem '
type: conference
volume: 529
year: '2000'
...
---
_id: '2342'
abstract:
- lang: eng
text: In the theoretical description of recent experiments with dilute Bose gases
confined in external potentials the Gross-Pitaevskii equation plays an important
role. Its status as an approximation for the quantum mechanical many-body ground
state problem has recently been rigorously clarified. A summary of this work is
presented here.
article_processing_charge: No
arxiv: 1
author:
- first_name: Robert
full_name: Seiringer, Robert
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
- first_name: Élliott
full_name: Lieb, Élliott
last_name: Lieb
- first_name: Jakob
full_name: Yngvason, Jakob
last_name: Yngvason
citation:
ama: 'Seiringer R, Lieb É, Yngvason J. The ground state energy and density of interacting
bosons in a trap. In: Proceedings of the International Symposium on Quantum
Theory and Symmetries. World Scientific Publishing; 2000:101-110.'
apa: Seiringer, R., Lieb, É., & Yngvason, J. (2000). The ground state energy
and density of interacting bosons in a trap. In Proceedings of the International
Symposium on Quantum Theory and Symmetries (pp. 101–110). World Scientific
Publishing.
chicago: Seiringer, Robert, Élliott Lieb, and Jakob Yngvason. “The Ground State
Energy and Density of Interacting Bosons in a Trap.” In Proceedings of the
International Symposium on Quantum Theory and Symmetries, 101–10. World Scientific
Publishing, 2000.
ieee: R. Seiringer, É. Lieb, and J. Yngvason, “The ground state energy and density
of interacting bosons in a trap,” in Proceedings of the International Symposium
on Quantum Theory and Symmetries, 2000, pp. 101–110.
ista: 'Seiringer R, Lieb É, Yngvason J. 2000. The ground state energy and density
of interacting bosons in a trap. Proceedings of the International Symposium on
Quantum Theory and Symmetries. ISQTS: Quantum Theory and Symmetries, 101–110.'
mla: Seiringer, Robert, et al. “The Ground State Energy and Density of Interacting
Bosons in a Trap.” Proceedings of the International Symposium on Quantum Theory
and Symmetries, World Scientific Publishing, 2000, pp. 101–10.
short: R. Seiringer, É. Lieb, J. Yngvason, in:, Proceedings of the International
Symposium on Quantum Theory and Symmetries, World Scientific Publishing, 2000,
pp. 101–110.
conference:
name: 'ISQTS: Quantum Theory and Symmetries'
date_created: 2018-12-11T11:57:06Z
date_published: 2000-06-01T00:00:00Z
date_updated: 2023-05-03T13:00:35Z
day: '01'
extern: '1'
external_id:
arxiv:
- math-ph/9911026
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://arxiv.org/abs/math-ph/9911026
month: '06'
oa: 1
oa_version: None
page: 101 - 110
publication: Proceedings of the International Symposium on Quantum Theory and Symmetries
publication_identifier:
isbn:
- '9789810242374 '
publication_status: published
publisher: World Scientific Publishing
publist_id: '4584'
quality_controlled: '1'
status: public
title: The ground state energy and density of interacting bosons in a trap
type: conference
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
year: '2000'
...
---
_id: '2343'
abstract:
- lang: eng
text: We study the energy levels of a single particle in a homogeneous magnetic
field and in an axially symmetric external potential. For potentials that are
superharmonic off the central axis, we find a general 'pseudoconcave' ordering
of the ground state energies of the Hamiltonian restricted to the sectors with
fixed angular momentum. The physical applications include atoms and ions in strong
magnetic fields. There the energies are monotone increasing and concave in angular
momentum. In the case of a periodic chain of atoms, the pseudoconcavity extends
to the entire lowest band of Bloch functions.
author:
- first_name: Bernhard
full_name: Baumgartner, Bernhard
last_name: Baumgartner
- first_name: Robert
full_name: Robert Seiringer
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
citation:
ama: Baumgartner B, Seiringer R. On the ordering of energy levels in homogeneous
magnetic fields. Letters in Mathematical Physics. 2000;54(3):213-226. doi: 10.1023/A:1010978807635
apa: Baumgartner, B., & Seiringer, R. (2000). On the ordering of energy levels
in homogeneous magnetic fields. Letters in Mathematical Physics. Springer.
https://doi.org/ 10.1023/A:1010978807635
chicago: Baumgartner, Bernhard, and Robert Seiringer. “On the Ordering of Energy
Levels in Homogeneous Magnetic Fields.” Letters in Mathematical Physics.
Springer, 2000. https://doi.org/
10.1023/A:1010978807635.
ieee: B. Baumgartner and R. Seiringer, “On the ordering of energy levels in homogeneous
magnetic fields,” Letters in Mathematical Physics, vol. 54, no. 3. Springer,
pp. 213–226, 2000.
ista: Baumgartner B, Seiringer R. 2000. On the ordering of energy levels in homogeneous
magnetic fields. Letters in Mathematical Physics. 54(3), 213–226.
mla: Baumgartner, Bernhard, and Robert Seiringer. “On the Ordering of Energy Levels
in Homogeneous Magnetic Fields.” Letters in Mathematical Physics, vol.
54, no. 3, Springer, 2000, pp. 213–26, doi:
10.1023/A:1010978807635.
short: B. Baumgartner, R. Seiringer, Letters in Mathematical Physics 54 (2000) 213–226.
date_created: 2018-12-11T11:57:06Z
date_published: 2000-11-01T00:00:00Z
date_updated: 2021-01-12T06:56:54Z
day: '01'
doi: ' 10.1023/A:1010978807635'
extern: 1
fulldoi: https://doi.org/ 10.1023/A:1010978807635
intvolume: ' 54'
issue: '3'
main_file_link:
- open_access: '1'
url: http://arxiv.org/abs/math-ph/0011031
month: '11'
oa: 1
page: 213 - 226
publication: Letters in Mathematical Physics
publication_status: published
publisher: Springer
publist_id: '4582'
quality_controlled: 0
status: public
title: On the ordering of energy levels in homogeneous magnetic fields
type: journal_article
volume: 54
year: '2000'
...
---
_id: '2344'
abstract:
- lang: eng
text: The ground-state properties of interacting Bose gases in external potentials,
as considered in recent experiments, are usually described by means of the Gross-Pitaevskii
energy functional. We present here a rigorous proof of the asymptotic exactness
of this approximation for the ground-state energy and particle density of a dilute
Bose gas with a positive interaction.
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Élliott
full_name: Lieb, Élliott
last_name: Lieb
- first_name: Robert
full_name: Seiringer, Robert
id: 4AFD0470-F248-11E8-B48F-1D18A9856A87
last_name: Seiringer
orcid: 0000-0002-6781-0521
- first_name: Jakob
full_name: Yngvason, Jakob
last_name: Yngvason
citation:
ama: 'Lieb É, Seiringer R, Yngvason J. Bosons in a trap: A rigorous derivation of
the Gross-Pitaevskii energy functional. Physical Review A - Atomic, Molecular,
and Optical Physics. 2000;61(4):436021-4360213. doi:10.1103/PhysRevA.61.043602'
apa: 'Lieb, É., Seiringer, R., & Yngvason, J. (2000). Bosons in a trap: A rigorous
derivation of the Gross-Pitaevskii energy functional. Physical Review A - Atomic,
Molecular, and Optical Physics. American Physical Society. https://doi.org/10.1103/PhysRevA.61.043602'
chicago: 'Lieb, Élliott, Robert Seiringer, and Jakob Yngvason. “Bosons in a Trap:
A Rigorous Derivation of the Gross-Pitaevskii Energy Functional.” Physical
Review A - Atomic, Molecular, and Optical Physics. American Physical Society,
2000. https://doi.org/10.1103/PhysRevA.61.043602.'
ieee: 'É. Lieb, R. Seiringer, and J. Yngvason, “Bosons in a trap: A rigorous derivation
of the Gross-Pitaevskii energy functional,” Physical Review A - Atomic, Molecular,
and Optical Physics, vol. 61, no. 4. American Physical Society, pp. 436021–4360213,
2000.'
ista: 'Lieb É, Seiringer R, Yngvason J. 2000. Bosons in a trap: A rigorous derivation
of the Gross-Pitaevskii energy functional. Physical Review A - Atomic, Molecular,
and Optical Physics. 61(4), 436021–4360213.'
mla: 'Lieb, Élliott, et al. “Bosons in a Trap: A Rigorous Derivation of the Gross-Pitaevskii
Energy Functional.” Physical Review A - Atomic, Molecular, and Optical Physics,
vol. 61, no. 4, American Physical Society, 2000, pp. 436021–4360213, doi:10.1103/PhysRevA.61.043602.'
short: É. Lieb, R. Seiringer, J. Yngvason, Physical Review A - Atomic, Molecular,
and Optical Physics 61 (2000) 436021–4360213.
date_created: 2018-12-11T11:57:07Z
date_published: 2000-04-01T00:00:00Z
date_updated: 2023-05-03T12:47:08Z
day: '01'
doi: 10.1103/PhysRevA.61.043602
extern: '1'
external_id:
arxiv:
- math-ph/9908027
fulldoi: https://doi.org/10.1103/PhysRevA.61.043602
intvolume: ' 61'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://arxiv.org/abs/math-ph/9908027
month: '04'
oa: 1
oa_version: Published Version
page: 436021 - 4360213
publication: Physical Review A - Atomic, Molecular, and Optical Physics
publication_identifier:
issn:
- 0556-2791
publication_status: published
publisher: American Physical Society
publist_id: '4583'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Bosons in a trap: A rigorous derivation of the Gross-Pitaevskii energy functional'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 61
year: '2000'
...
---
_id: '2418'
abstract:
- lang: eng
text: For an absolutely continuous probability measure μ on Rd and a nonnegative
integer k, let sk(μ, 0) denote the probability that the convex hull of k+d+1 random
points which are i.i.d. according to μ contains the origin 0. For d and k given,
we determine a tight upper bound on sk(μ, 0), and we characterize the measures
in Rd which attain this bound. This result can be considered a continuous analogue
of the Upper Bound Theorem for the maximal number of faces of convex polytopes
with a given number of vertices. For our proof we introduce so-called h-functions,
continuous counterparts of h-vectors for simplicial convex polytopes.
article_processing_charge: No
author:
- first_name: Uli
full_name: Wagner, Uli
id: 36690CA2-F248-11E8-B48F-1D18A9856A87
last_name: Wagner
orcid: 0000-0002-1494-0568
- first_name: Emo
full_name: Welzl, Emo
last_name: Welzl
citation:
ama: 'Wagner U, Welzl E. Origin-embracing distributions or a continuous analogue
of the Upper Bound Theorem. In: Proceedings of the 16th Annual Symposium on
Computational Geometry. ACM; 2000:50-56. doi:10.1145/336154.336176'
apa: 'Wagner, U., & Welzl, E. (2000). Origin-embracing distributions or a continuous
analogue of the Upper Bound Theorem. In Proceedings of the 16th annual symposium
on Computational geometry (pp. 50–56). Clear Water Bay Kowloon, Hong Kong:
ACM. https://doi.org/10.1145/336154.336176'
chicago: Wagner, Uli, and Emo Welzl. “Origin-Embracing Distributions or a Continuous
Analogue of the Upper Bound Theorem.” In Proceedings of the 16th Annual Symposium
on Computational Geometry, 50–56. ACM, 2000. https://doi.org/10.1145/336154.336176.
ieee: U. Wagner and E. Welzl, “Origin-embracing distributions or a continuous analogue
of the Upper Bound Theorem,” in Proceedings of the 16th annual symposium on
Computational geometry, Clear Water Bay Kowloon, Hong Kong, 2000, pp. 50–56.
ista: 'Wagner U, Welzl E. 2000. Origin-embracing distributions or a continuous analogue
of the Upper Bound Theorem. Proceedings of the 16th annual symposium on Computational
geometry. SCG: Symposium on Computational Geometry, 50–56.'
mla: Wagner, Uli, and Emo Welzl. “Origin-Embracing Distributions or a Continuous
Analogue of the Upper Bound Theorem.” Proceedings of the 16th Annual Symposium
on Computational Geometry, ACM, 2000, pp. 50–56, doi:10.1145/336154.336176.
short: U. Wagner, E. Welzl, in:, Proceedings of the 16th Annual Symposium on Computational
Geometry, ACM, 2000, pp. 50–56.
conference:
end_date: 2000-04-14
location: Clear Water Bay Kowloon, Hong Kong
name: 'SCG: Symposium on Computational Geometry'
start_date: 2000-06-12
date_created: 2018-12-11T11:57:33Z
date_published: 2000-05-01T00:00:00Z
date_updated: 2023-05-03T12:41:02Z
day: '01'
doi: 10.1145/336154.336176
extern: '1'
fulldoi: https://doi.org/10.1145/336154.336176
language:
- iso: eng
month: '05'
oa_version: None
page: 50 - 56
publication: Proceedings of the 16th annual symposium on Computational geometry
publication_identifier:
isbn:
- '9781581132243'
publication_status: published
publisher: ACM
publist_id: '4507'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Origin-embracing distributions or a continuous analogue of the Upper Bound
Theorem
type: conference
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
year: '2000'
...
---
_id: '2494'
abstract:
- lang: eng
text: 'This chapter focuses on metabotropic glutamate receptors (mGluRs). These
receptors are linked to several intracellular signal transduction mechanisms via
G-protein and are implicated in diverse functions of the mammalian central nervous
system (CNS). These functions include mediation of slow excitatory and inhibitory
responses; regulation of calcium channels, potassium channels, and non-selective
cation channels; inhibition and facilitation of transmitter release; induction
of long-term potentiation and long-term depression; and regulation of neuronal
development. Functional diversity of the metabotropic glutamate receptor is reflected
in molecular diversity of receptor subtypes. The sites of action of mGluRs are
widely found throughout different membrane compartments of neuronal and glial
cells. The chapter reviews the differential subcellular localization of metabotropic
glutamate receptors in relation to transmitter release sites. Patterns of subcellular
localization of mGluRs are different among three subgroups: group I and III mGluRs
are mainly localized to somatodendritic and axonal domains of neurons, respectively,
while group II mGluRs are extensively localized to both domains as well as to
glial cell processes.'
alternative_title:
- Handbook of Chemical Neuroanatomy
article_processing_charge: No
author:
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Shigemoto R, Mizuno N. Chapter III Metabotropic glutamate receptors - immunocytochemical
and in situ hybridization analyses. In: Glutamate. Vol 18. Elsevier; 2000:63-98.
doi:10.1016/S0924-8196(00)80044-5'
apa: Shigemoto, R., & Mizuno, N. (2000). Chapter III Metabotropic glutamate
receptors - immunocytochemical and in situ hybridization analyses. In Glutamate
(Vol. 18, pp. 63–98). Elsevier. https://doi.org/10.1016/S0924-8196(00)80044-5
chicago: Shigemoto, Ryuichi, and Noboru Mizuno. “Chapter III Metabotropic Glutamate
Receptors - Immunocytochemical and in Situ Hybridization Analyses.” In Glutamate,
18:63–98. Elsevier, 2000. https://doi.org/10.1016/S0924-8196(00)80044-5.
ieee: R. Shigemoto and N. Mizuno, “Chapter III Metabotropic glutamate receptors
- immunocytochemical and in situ hybridization analyses,” in Glutamate,
vol. 18, Elsevier, 2000, pp. 63–98.
ista: 'Shigemoto R, Mizuno N. 2000.Chapter III Metabotropic glutamate receptors
- immunocytochemical and in situ hybridization analyses. In: Glutamate. Handbook
of Chemical Neuroanatomy, vol. 18, 63–98.'
mla: Shigemoto, Ryuichi, and Noboru Mizuno. “Chapter III Metabotropic Glutamate
Receptors - Immunocytochemical and in Situ Hybridization Analyses.” Glutamate,
vol. 18, Elsevier, 2000, pp. 63–98, doi:10.1016/S0924-8196(00)80044-5.
short: R. Shigemoto, N. Mizuno, in:, Glutamate, Elsevier, 2000, pp. 63–98.
date_created: 2018-12-11T11:58:00Z
date_published: 2000-01-01T00:00:00Z
date_updated: 2023-05-03T12:27:40Z
day: '01'
doi: 10.1016/S0924-8196(00)80044-5
extern: '1'
fulldoi: https://doi.org/10.1016/S0924-8196(00)80044-5
intvolume: ' 18'
language:
- iso: eng
month: '01'
oa_version: None
page: 63 - 98
publication: Glutamate
publication_identifier:
eissn:
- 0924-8196
publication_status: published
publisher: Elsevier
publist_id: '4407'
quality_controlled: '1'
status: public
title: Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ
hybridization analyses
type: book_chapter
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 18
year: '2000'
...
---
_id: '2598'
abstract:
- lang: eng
text: "There are many types of cerebellar ataxia, including ataxia due to congenital
or metabolic disorders and a paraneoplastic form in patients with gynecologic
cancer, breast cancer, lung cancer, or Hodgkin's disease.1 This paraneoplastic
syndrome is the only type of cerebellar ataxia associated with autoantibodies
against neuronal antigens. Often, the neuronal antigens are aberrantly expressed
by the tumor cells.2-4 The antineuronal autoantibodies are believed to cause cerebellar
ataxia, but this is unproved.5,6 In Hodgkin's disease, the lymphoma precedes the
ataxia by months to years in 80 percent of patients, and ataxia often occurs during
a prolonged complete remission.4 Among patients with this type of ataxia, 30 percent
have anti–Purkinje-cell antibodies, some of which have the features of the neuronal
antibody anti-Tr.4,7\r\n\r\nWe identified a new autoantibody in two patients with
severe cerebellar ataxia that developed while they were in remission from Hodgkin's
disease. The antibody reacts specifically with the metabotropic glutamate receptor
mGluR1 in mouse brain. Metabotropic glutamate receptors belong to a large family
of cell-surface receptors that transmit signals into the cell by coupling to guanine
nucleotide-binding proteins (G proteins) in the cytoplasm. Purified IgG from the
serum of both patients blocked the glutamate-stimulated formation of inositol
phosphates in Chinese-hamster-ovary (CHO) cells that expressed mGluR1α, and the
injection of IgG from serum or cerebrospinal fluid into the cerebellar subarachnoid
space of mice caused severe, reversible ataxia. These results indicate that antineuronal
autoantibodies can cause disease of the central nervous system by blocking neuronal
receptors."
acknowledgement: "Supported in part by the Ministry of Education, Science, and Culture
of Japan (Dr. Nakanishi and Dr. Shigemoto); CREST of Japan Science and Technology
Corporation (Dr. Shigemoto); the Life Sciences Foundation (Dr. De Zeeuw); NWO (Dr.
De Zeeuw and Dr. De Leeuw); and the Human Frontier Science Program (Dr. De Zeeuw
and Dr. Shigemoto).\r\nDrs. Sillevis Smitt and Kinoshita contributed equally to
the article. We are indebted to A. Aiba for providing mGluR1-deficient mice; to
T. Maruyama for providing human mGluR1-expressing CHO cells; to H. Jingami for helpful
discussion; to A. Uesugi for photographic assistance; to M. van den Bent and C.
Gaillard for clinical information on the patients; and to J. van der Burg, S.K.E.
Koekkoek, K.J. Reus, and C. Vermeer for technical assistance."
article_processing_charge: No
article_type: original
author:
- first_name: Peter
full_name: Sillevis Smitt, Peter
last_name: Sillevis Smitt
- first_name: Ayae
full_name: Kinoshita, Ayae
last_name: Kinoshita
- first_name: Bertie
full_name: De Leeuw, Bertie
last_name: De Leeuw
- first_name: Wiebe
full_name: Moll, Wiebe
last_name: Moll
- first_name: Michiel
full_name: Coesmans, Michiel
last_name: Coesmans
- first_name: Dick
full_name: Jaarsma, Dick
last_name: Jaarsma
- first_name: Sonja
full_name: Henzen Logmans, Sonja
last_name: Henzen Logmans
- first_name: Charles
full_name: Vecht, Charles
last_name: Vecht
- first_name: Chris
full_name: De Zeeuw, Chris
last_name: De Zeeuw
- first_name: Naotaka
full_name: Sekiyama, Naotaka
last_name: Sekiyama
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Sillevis Smitt P, Kinoshita A, De Leeuw B, et al. Paraneoplastic cerebellar
ataxia due to autoantibodies against a glutamate receptor. New England Journal
of Medicine. 2000;342(1):21-27. doi:10.1056/NEJM200001063420104
apa: Sillevis Smitt, P., Kinoshita, A., De Leeuw, B., Moll, W., Coesmans, M., Jaarsma,
D., … Shigemoto, R. (2000). Paraneoplastic cerebellar ataxia due to autoantibodies
against a glutamate receptor. New England Journal of Medicine. Massachussetts
Medical Society. https://doi.org/10.1056/NEJM200001063420104
chicago: Sillevis Smitt, Peter, Ayae Kinoshita, Bertie De Leeuw, Wiebe Moll, Michiel
Coesmans, Dick Jaarsma, Sonja Henzen Logmans, et al. “Paraneoplastic Cerebellar
Ataxia Due to Autoantibodies against a Glutamate Receptor.” New England Journal
of Medicine. Massachussetts Medical Society, 2000. https://doi.org/10.1056/NEJM200001063420104.
ieee: P. Sillevis Smitt et al., “Paraneoplastic cerebellar ataxia due to
autoantibodies against a glutamate receptor,” New England Journal of Medicine,
vol. 342, no. 1. Massachussetts Medical Society, pp. 21–27, 2000.
ista: Sillevis Smitt P, Kinoshita A, De Leeuw B, Moll W, Coesmans M, Jaarsma D,
Henzen Logmans S, Vecht C, De Zeeuw C, Sekiyama N, Nakanishi S, Shigemoto R. 2000.
Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor.
New England Journal of Medicine. 342(1), 21–27.
mla: Sillevis Smitt, Peter, et al. “Paraneoplastic Cerebellar Ataxia Due to Autoantibodies
against a Glutamate Receptor.” New England Journal of Medicine, vol. 342,
no. 1, Massachussetts Medical Society, 2000, pp. 21–27, doi:10.1056/NEJM200001063420104.
short: P. Sillevis Smitt, A. Kinoshita, B. De Leeuw, W. Moll, M. Coesmans, D. Jaarsma,
S. Henzen Logmans, C. Vecht, C. De Zeeuw, N. Sekiyama, S. Nakanishi, R. Shigemoto,
New England Journal of Medicine 342 (2000) 21–27.
date_created: 2018-12-11T11:58:35Z
date_published: 2000-01-06T00:00:00Z
date_updated: 2023-05-03T11:14:19Z
day: '06'
doi: 10.1056/NEJM200001063420104
extern: '1'
external_id:
pmid:
- '10620645'
fulldoi: https://doi.org/10.1056/NEJM200001063420104
intvolume: ' 342'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.nejm.org/doi/full/10.1056/nejm200001063420104
month: '01'
oa: 1
oa_version: None
page: 21 - 27
pmid: 1
publication: New England Journal of Medicine
publication_identifier:
issn:
- 0028-4793
publication_status: published
publisher: Massachussetts Medical Society
publist_id: '4300'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate
receptor
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 342
year: '2000'
...
---
_id: '2599'
abstract:
- lang: eng
text: 'The synaptic relationship between substance P (SP) and its receptor, i.e.,
neurokinin-1 receptor (NK1R), was examined in the striatum of the rat by confocal
laser-scanning microscopy and electron microscopy. For confocal laser-scanning
microscopy, triple-immunofluorescence histochemistry was performed to label NK1R,
SP, and vesicular acetylcholine transporter (a specific marker for cholinergic
neurons). In electron microscopic double- immunolabeling study, immunoreactivity
for NK1R was detected with the silver- intensified gold method, while immunoreactivity
for SP was detected with peroxidase immunohistochemistry. Simultaneous immunolabeling
of NK1R and SP revealed significant mismatch at the synaptic level: although some
SP- immunopositive axon terminals were in synaptic contact with NK1R- immunopositive
sites of plasma membrane, NK1R-immunoreactivity was observed at both synaptic
and non-synaptic sites of plasma membrane. Thus, SP released from the sites remote
from NK1Rs might diffuse in the extracellular fluid to act, as a paracrine neurotransmitter,
on NK1Rs distant from its releasing site. SP neurotransmission in the striatum
might occur not only synaptically but also extrasynaptically. The SP-NK1R system
might constitute an association system within the striatum.'
acknowledgement: The authors are grateful for the photographic help of Ms. Yue-Ping Yuan, Mr. Akira Uesugi, Ms. Keiko Oka-moto,
Mr. Nobuyuki Kobayashi, and Mr. Hideki Itabashi. The authors also express their
gratitudes for the support of Dr. Kajitaro Morita in Morita Clinic of Internal Medicine
and Pediatrics, Kadoma, Osaka, Japan
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Li, Jin
last_name: Li
- first_name: Dan
full_name: Wang, Dan
last_name: Wang
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. Relationship between
neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic
immunohistochemical study in the rat. Journal of Comparative Neurology.
2000;418(2):156-163. doi:10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z'
apa: 'Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N.
(2000). Relationship between neurokinin-1 receptor and substance P in the striatum:
Light and electron microscopic immunohistochemical study in the rat. Journal
of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z'
chicago: 'Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura,
and Noboru Mizuno. “Relationship between Neurokinin-1 Receptor and Substance P
in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the
Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 2000. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z.'
ieee: 'J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “Relationship
between neurokinin-1 receptor and substance P in the striatum: Light and electron
microscopic immunohistochemical study in the rat,” Journal of Comparative Neurology,
vol. 418, no. 2. Wiley-Blackwell, pp. 156–163, 2000.'
ista: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. Relationship
between neurokinin-1 receptor and substance P in the striatum: Light and electron
microscopic immunohistochemical study in the rat. Journal of Comparative Neurology.
418(2), 156–163.'
mla: 'Li, Jin, et al. “Relationship between Neurokinin-1 Receptor and Substance
P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in
the Rat.” Journal of Comparative Neurology, vol. 418, no. 2, Wiley-Blackwell,
2000, pp. 156–63, doi:10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z.'
short: J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Journal of
Comparative Neurology 418 (2000) 156–163.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-03-06T00:00:00Z
date_updated: 2023-05-03T10:56:44Z
day: '06'
doi: 10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z
extern: '1'
external_id:
pmid:
- '10701441'
fulldoi: https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z
intvolume: ' 418'
issue: '2'
language:
- iso: eng
month: '03'
oa_version: None
page: 156 - 163
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4299'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relationship between neurokinin-1 receptor and substance P in the striatum:
Light and electron microscopic immunohistochemical study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 418
year: '2000'
...
---
_id: '2600'
abstract:
- lang: eng
text: 'The synaptic relationship between substance P (SP) and its receptor, i.e.
neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal
subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the
rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry
for NK1 and SP was performed. In electron microscopic double-immunolabeling study,
immunoreactivity for NK1R was detected with the silver-intensified gold method,
while immunoreactivity for SP was detected with peroxidase immunohistochemistry.
SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric)
contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo.
Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive
sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic
and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals
might not only act on NK1Rs facing the SP-containing axon terminals, but also
diffuse in the extracellular fluid for distances larger than the synaptic cleft
to act on NK1Rs at some distances from the synaptic sites. '
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Li, Jin
last_name: Li
- first_name: Dan
full_name: Wang, Dan
last_name: Wang
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. The relationship
between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
light and electron microscopic immunohistochemical study in the rat. Neuroscience
Research. 2000;36(4):327-334. doi:10.1016/S0168-0102(00)00095-X'
apa: 'Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N.
(2000). The relationship between neurokinin-1 receptor and substance P in the
medullary dorsal horn: A light and electron microscopic immunohistochemical study
in the rat. Neuroscience Research. Elsevier. https://doi.org/10.1016/S0168-0102(00)00095-X'
chicago: 'Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura,
and Noboru Mizuno. “The Relationship between Neurokinin-1 Receptor and Substance
P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical
Study in the Rat.” Neuroscience Research. Elsevier, 2000. https://doi.org/10.1016/S0168-0102(00)00095-X.'
ieee: 'J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “The relationship
between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
light and electron microscopic immunohistochemical study in the rat,” Neuroscience
Research, vol. 36, no. 4. Elsevier, pp. 327–334, 2000.'
ista: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. The relationship
between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
light and electron microscopic immunohistochemical study in the rat. Neuroscience
Research. 36(4), 327–334.'
mla: 'Li, Jin, et al. “The Relationship between Neurokinin-1 Receptor and Substance
P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical
Study in the Rat.” Neuroscience Research, vol. 36, no. 4, Elsevier, 2000,
pp. 327–34, doi:10.1016/S0168-0102(00)00095-X.'
short: J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Neuroscience
Research 36 (2000) 327–334.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-04-01T00:00:00Z
date_updated: 2023-05-03T10:47:06Z
day: '01'
doi: 10.1016/S0168-0102(00)00095-X
extern: '1'
external_id:
pmid:
- '10771111'
fulldoi: https://doi.org/10.1016/S0168-0102(00)00095-X
intvolume: ' 36'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 327 - 334
pmid: 1
publication: Neuroscience Research
publication_identifier:
issn:
- 0168-0102
publication_status: published
publisher: Elsevier
publist_id: '4298'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'The relationship between neurokinin-1 receptor and substance P in the medullary
dorsal horn: A light and electron microscopic immunohistochemical study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 36
year: '2000'
...
---
_id: '2601'
abstract:
- lang: eng
text: Targeted deletion of metabotropic glutamate receptor-subtype 1 (mGluR1) gene
can cause defects in development and function in the cerebellum. We introduced
the mGluR1α transgene into mGluR1-null mutant [mGluR1 (-/-)] mice with a Purkinje
cell (PC)-specific promoter. mGluR1-rescue mice showed normal cerebellar long-term
depression and regression of multiple climbing fiber innervation, events significantly
impaired in mGluR1 (-/-) mice. The impaired motor coordination was rescued by
this transgene, in a dose-dependent manner. We propose that mGluR1 in PCs is a
key molecule for normal synapse formation, synaptic plasticity, and motor control
in the cerebellum.
article_processing_charge: No
article_type: original
author:
- first_name: Taeko
full_name: Ichise, Taeko
last_name: Ichise
- first_name: Masanobu
full_name: Kano, Masanobu
last_name: Kano
- first_name: Kouichi
full_name: Hashimoto, Kouichi
last_name: Hashimoto
- first_name: Dai
full_name: Yanagihara, Dai
last_name: Yanagihara
- first_name: Kazuki
full_name: Nakao, Kazuki
last_name: Nakao
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Motoya
full_name: Katsuki, Motoya
last_name: Katsuki
- first_name: Atsu
full_name: Aiba, Atsu
last_name: Aiba
citation:
ama: Ichise T, Kano M, Hashimoto K, et al. mGluR1 in cerebellar Purkinje cells essential
for long-term depression, synapse elimination, and motor coordination. Science.
2000;288(5472):1832-1835. doi:10.1126/science.288.5472.1832
apa: Ichise, T., Kano, M., Hashimoto, K., Yanagihara, D., Nakao, K., Shigemoto,
R., … Aiba, A. (2000). mGluR1 in cerebellar Purkinje cells essential for long-term
depression, synapse elimination, and motor coordination. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.288.5472.1832
chicago: Ichise, Taeko, Masanobu Kano, Kouichi Hashimoto, Dai Yanagihara, Kazuki
Nakao, Ryuichi Shigemoto, Motoya Katsuki, and Atsu Aiba. “MGluR1 in Cerebellar
Purkinje Cells Essential for Long-Term Depression, Synapse Elimination, and Motor
Coordination.” Science. American Association for the Advancement of Science,
2000. https://doi.org/10.1126/science.288.5472.1832.
ieee: T. Ichise et al., “mGluR1 in cerebellar Purkinje cells essential for
long-term depression, synapse elimination, and motor coordination,” Science,
vol. 288, no. 5472. American Association for the Advancement of Science, pp. 1832–1835,
2000.
ista: Ichise T, Kano M, Hashimoto K, Yanagihara D, Nakao K, Shigemoto R, Katsuki
M, Aiba A. 2000. mGluR1 in cerebellar Purkinje cells essential for long-term depression,
synapse elimination, and motor coordination. Science. 288(5472), 1832–1835.
mla: Ichise, Taeko, et al. “MGluR1 in Cerebellar Purkinje Cells Essential for Long-Term
Depression, Synapse Elimination, and Motor Coordination.” Science, vol.
288, no. 5472, American Association for the Advancement of Science, 2000, pp.
1832–35, doi:10.1126/science.288.5472.1832.
short: T. Ichise, M. Kano, K. Hashimoto, D. Yanagihara, K. Nakao, R. Shigemoto,
M. Katsuki, A. Aiba, Science 288 (2000) 1832–1835.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-06-09T00:00:00Z
date_updated: 2023-05-03T09:53:38Z
day: '09'
doi: 10.1126/science.288.5472.1832
extern: '1'
external_id:
pmid:
- '10846166 '
fulldoi: https://doi.org/10.1126/science.288.5472.1832
intvolume: ' 288'
issue: '5472'
language:
- iso: eng
month: '06'
oa_version: None
page: 1832 - 1835
pmid: 1
publication: Science
publication_identifier:
issn:
- 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '4297'
quality_controlled: '1'
scopus_import: '1'
status: public
title: mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse
elimination, and motor coordination
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 288
year: '2000'
...
---
_id: '2602'
abstract:
- lang: eng
text: Although presynaptic localization of mGluR7 is well established, the mechanism
by which the receptor may control Ca2+ channels in neurons is still unknown. We
show here that cultured cerebellar granule cells express native metabotropic glutamate
receptor type 7 (mGluR7) in neuritic processes, whereas transfected mGluR7 was
also expressed in cell bodies. This allowed us to study the effect of the transfected
receptor on somatic Ca2+ channels. In transfected neurons, mGuR7 selectively inhibited
P/Q-type Ca2+ channels. The effect was mimicked by GTPγS and blocked by pertussis
toxin (PTX) or a selective antibody raised against the G-protein αo subunit, indicating
the involvement of a G(o)-like protein. The mGuR7 effect did not display the characteristics
of a direct interaction between G-protein βγ subunits and the α1A Ca2+ channel
subunit, but was abolished by quenching βγ subunits with specific intracellular
peptides. Intracellular dialysis of G-protein βγ subunits did not mimic the action
of mGluR7, suggesting that both G-protein βγ and αo subunits were required to
mediate the effect. Inhibition of phospholipase C (PLC) blocked the inhibitory
action of mGluR7, suggesting that a coincident activation of PLC by the G-protein
βγ with αo subunits was required. The Ca2+ chelator BAPTA, as well as inhibition
of either the inositol trisphosphate (IP3) receptor or protein kinase C (PKC)
abolished the mGluR7 effect. Moreover, activation of native mGluR7 induced a PTX-dependent
IP3 formation. These results indicated that IP3-mediated intracellular Ca2+ release
was required for PKC-dependent inhibition of the Ca2+ channels. Possible control
of synaptic transmission by the present mechanisms is discussed.
acknowledgement: This work was supported by Centre National de la Recherche Scientifique
and grants from Association Française contre les Myopathies, Fondation pour la Recherche
Médicale, Bayer (France), and Hoechst-Marrion-Roussel (FRHMR1/9702). We thank J.
P. Pin and F. Ango for constructive discussion of this work. We also thank Dr. J.
Saugstad (Atlanta, GA) for the rat mGluR7a cDNA, J. M. Sabatier (Marseille, France)
for the synthesis of the 68 AA peptide, V. Homburger (Montpellier, France) for the
anti-Gαo antibody, and B. Mouillac (Montpellier, France) for the anti-cMyc monoclonal
antibody.
article_processing_charge: No
article_type: original
author:
- first_name: Julie
full_name: Perroy, Julie
last_name: Perroy
- first_name: Laurent
full_name: Prezèau, Laurent
last_name: Prezèau
- first_name: Michel
full_name: De Waard, Michel
last_name: De Waard
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Joël
full_name: Bockaërt, Joël
last_name: Bockaërt
- first_name: Laurent
full_name: Fagni, Laurent
last_name: Fagni
citation:
ama: Perroy J, Prezèau L, De Waard M, Shigemoto R, Bockaërt J, Fagni L. Selective
blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type
7 involves a phospholipase C pathway in neurons. Journal of Neuroscience.
2000;20(21):7896-7904. doi:10.1523/JNEUROSCI.20-21-07896.2000
apa: Perroy, J., Prezèau, L., De Waard, M., Shigemoto, R., Bockaërt, J., & Fagni,
L. (2000). Selective blockade of P/Q-type calcium channels by the metabotropic
glutamate receptor type 7 involves a phospholipase C pathway in neurons. Journal
of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000
chicago: Perroy, Julie, Laurent Prezèau, Michel De Waard, Ryuichi Shigemoto, Joël
Bockaërt, and Laurent Fagni. “Selective Blockade of P/Q-Type Calcium Channels
by the Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway
in Neurons.” Journal of Neuroscience. Society for Neuroscience, 2000. https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000.
ieee: J. Perroy, L. Prezèau, M. De Waard, R. Shigemoto, J. Bockaërt, and L. Fagni,
“Selective blockade of P/Q-type calcium channels by the metabotropic glutamate
receptor type 7 involves a phospholipase C pathway in neurons,” Journal of
Neuroscience, vol. 20, no. 21. Society for Neuroscience, pp. 7896–7904, 2000.
ista: Perroy J, Prezèau L, De Waard M, Shigemoto R, Bockaërt J, Fagni L. 2000. Selective
blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type
7 involves a phospholipase C pathway in neurons. Journal of Neuroscience. 20(21),
7896–7904.
mla: Perroy, Julie, et al. “Selective Blockade of P/Q-Type Calcium Channels by the
Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway in Neurons.”
Journal of Neuroscience, vol. 20, no. 21, Society for Neuroscience, 2000,
pp. 7896–904, doi:10.1523/JNEUROSCI.20-21-07896.2000.
short: J. Perroy, L. Prezèau, M. De Waard, R. Shigemoto, J. Bockaërt, L. Fagni,
Journal of Neuroscience 20 (2000) 7896–7904.
date_created: 2018-12-11T11:58:37Z
date_published: 2000-11-01T00:00:00Z
date_updated: 2023-05-03T09:48:17Z
day: '01'
doi: 10.1523/JNEUROSCI.20-21-07896.2000
extern: '1'
external_id:
pmid:
- '11050109'
fulldoi: https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000
intvolume: ' 20'
issue: '21'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6772734/
month: '11'
oa: 1
oa_version: Published Version
page: 7896 - 7904
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
issn:
- 0270-6474
publication_status: published
publisher: Society for Neuroscience
publist_id: '4296'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Selective blockade of P/Q-type calcium channels by the metabotropic glutamate
receptor type 7 involves a phospholipase C pathway in neurons
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 20
year: '2000'
...
---
_id: '2603'
abstract:
- lang: eng
text: Aggregation of neurotransmitter receptors at pre- and postsynaptic structures
is crucial for efficient neuronal communication. In contrast to the wealth of
information about postsynaptic specializations, little is known about the molecular
organization of presynaptic membrane proteins. We show here that the metabotropic
glutamate receptor mGluR7a, which localizes specifically to presynaptic active
zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous
systems induces coclustering dependent upon the extreme C terminus of mGluR7a
and the PDZ domain of PICK1. mGluR7a and PICK1 localize to excitatory synapses
in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic
sites, mGluR7aΔ3 lacking the PICK1 binding site targets to axons but does not
cluster. These results suggest that PICK1 is a component of the presynaptic machinery
involved in mGlUR7a aggregation and in modulation of glutamate neurotransmission.
acknowledgement: 'We thank Drs. S. Nakanishi, K. Moriyoshi, V. I. Gelfand, and P.
J. Conn for gifts of cDNAs and antibodies, A. S. Serpinskaya and H. Wu for excellent
preparation of neuron cultures, and H. J. Chung for help in immunoblots. This work
was supported by the Pew Chari-table Trust and National Institutes of Health grants
NS33184 and NS39286 (A. M. C.), K02MH01152 and NIDA DA10309 (P. W.), and a fellowship
from IPSEN Foundation (H. B.). '
article_processing_charge: No
article_type: original
author:
- first_name: Hélène
full_name: Boudin, Hélène
last_name: Boudin
- first_name: Andrew
full_name: Doan, Andrew
last_name: Doan
- first_name: Jun
full_name: Xia, Jun
last_name: Xia
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Richard
full_name: Huganir, Richard
last_name: Huganir
- first_name: Paul
full_name: Worley, Paul
last_name: Worley
- first_name: Ann
full_name: Craig, Ann
last_name: Craig
citation:
ama: Boudin H, Doan A, Xia J, et al. Presynaptic clustering of mGluR7a requires
the PICK1 PDZ domain binding site. Neuron. 2000;28(2):485-497. doi:10.1016/S0896-6273(00)00127-6
apa: Boudin, H., Doan, A., Xia, J., Shigemoto, R., Huganir, R., Worley, P., &
Craig, A. (2000). Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain
binding site. Neuron. Elsevier. https://doi.org/10.1016/S0896-6273(00)00127-6
chicago: Boudin, Hélène, Andrew Doan, Jun Xia, Ryuichi Shigemoto, Richard Huganir,
Paul Worley, and Ann Craig. “Presynaptic Clustering of MGluR7a Requires the PICK1
PDZ Domain Binding Site.” Neuron. Elsevier, 2000. https://doi.org/10.1016/S0896-6273(00)00127-6.
ieee: H. Boudin et al., “Presynaptic clustering of mGluR7a requires the PICK1
PDZ domain binding site,” Neuron, vol. 28, no. 2. Elsevier, pp. 485–497,
2000.
ista: Boudin H, Doan A, Xia J, Shigemoto R, Huganir R, Worley P, Craig A. 2000.
Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site.
Neuron. 28(2), 485–497.
mla: Boudin, Hélène, et al. “Presynaptic Clustering of MGluR7a Requires the PICK1
PDZ Domain Binding Site.” Neuron, vol. 28, no. 2, Elsevier, 2000, pp. 485–97,
doi:10.1016/S0896-6273(00)00127-6.
short: H. Boudin, A. Doan, J. Xia, R. Shigemoto, R. Huganir, P. Worley, A. Craig,
Neuron 28 (2000) 485–497.
date_created: 2018-12-11T11:58:37Z
date_published: 2000-11-01T00:00:00Z
date_updated: 2023-05-03T09:41:55Z
day: '01'
doi: 10.1016/S0896-6273(00)00127-6
extern: '1'
external_id:
pmid:
- '11144358'
fulldoi: https://doi.org/10.1016/S0896-6273(00)00127-6
intvolume: ' 28'
issue: '2'
language:
- iso: eng
month: '11'
oa_version: None
page: 485 - 497
pmid: 1
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
publist_id: '4295'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 28
year: '2000'
...