@article{7293, abstract = {If proton exchange membrane fuel cells (PEMFC) are ever to succeed in sustainable energy landscape as a potential zero emission technology, it is inevitable to reduce electricity production cost associated mainly with its MEAs, cell hardware and gas storage units. We demonstrate a diverse strategy for achieving this target with a concomitant amplification of its specific energy and power, by rolling a thin graphene oxide (GO) based MEA alone into a tubular and air breathing architecture with internal fuel storage. The unique properties of GO being a barrier for molecular fuels and proton conducting to construct a GO based cylindrical MEA. This makes the tubular PEMFC ∼75 times lighter, featuring ∼37 and ∼92 times respectively, the power and energy per overall weight, making it a potential candidate for portable applications. The intrinsic electrochemical kinetics at the three-phase boundary are somewhat affected by the bending of the MEA, albeit at overall reduction in power production cost.}, author = {Thimmappa, Ravikumar and Chattanahalli Devendrachari, Mruthyunjayachari and Shafi, Shahid and Freunberger, Stefan Alexander and Ottakam Thotiyl, Musthafa}, issn = {0360-3199}, journal = {International Journal of Hydrogen Energy}, number = {47}, pages = {22305--22315}, publisher = {Elsevier}, title = {{Proton conducting hollow graphene oxide cylinder as molecular fuel barrier for tubular H2-air fuel cell}}, doi = {10.1016/j.ijhydene.2016.08.057}, volume = {41}, year = {2016}, } @article{7294, abstract = {Mesoporous nanocrystalline TiO2 and TiO2–V2O5 microspheres were prepared by non-hydrolytic sol–gel from TiCl4, VOCl3, and iPr2O at 110 °C without any solvent or additives. The samples were characterized by elemental analysis, X-ray diffraction, Raman spectroscopy, scanning electron microscopy, nitrogen physisorption, and impedance measurements. At low vanadium loadings, only TiO2 anatase was detected, and V2O5 scherbinaite was also detected at high vanadium loadings. The texture of the samples depended on the V loading, but all the samples appeared built of primary nanoparticles (≈10–20 nm in size) that aggregate to form mesoporous micron-sized spheres. The lithium insertion properties of these materials were evaluated by galvanostatic measurements taken using coin-type cells, in view of their application as electrode for rechargeable Li-ion batteries. The mesoporous TiO2 microspheres showed good performances, with a specific reversible capacity of 145 and 128 mAh g−1 at C/2 and C, respectively (C = 335.6 mA g−1), good coulombic efficiency, and a moderate capacity fade (6 %) from the 2nd to the 20th cycle at C/20. Although the addition of V effectively increased the electronic conductivity of the powders, the specific reversible capacity and cycling performances of the TiO2–V2O5 samples were only minimally improved for a 5 at% V loading and were lower at higher V loading.}, author = {Escamilla-Pérez, A. M. and Louvain, N. and Kaschowitz, M. and Freunberger, Stefan Alexander and Fontaine, O. and Boury, B. and Brun, N. and Mutin, P. H.}, issn = {0928-0707}, journal = {Journal of Sol-Gel Science and Technology}, number = {2}, pages = {270--278}, publisher = {Springer Nature}, title = {{Lithium insertion properties of mesoporous nanocrystalline TiO2 and TiO2–V2O5 microspheres prepared by non-hydrolytic sol–gel}}, doi = {10.1007/s10971-016-4037-9}, volume = {79}, year = {2016}, } @article{7295, abstract = {Redox ionic liquids consisting of ions bearing redox moieties are receiving increasing interest in electrochemical applications, as they associate electroactive properties with the classical properties of ionic liquids. Here, biredox ionic liquid electrolytes are described in which both anion and cation are functionalized with anthraquinone and 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO) groups, respectively. In-depth investigations based on crossed experimental and theoretical studies were carried out to elucidate how the bulkiness of ions bearing a redox moiety impacted electron and mass transfers, and accordingly the efficiency of electrochemical devices. The values of solvated radii of different redox ions, as well as the related kinetic constants, were extracted from cyclic voltammetry experiments. Reformulating the basic relations of electron transfer theory (based on Marcus-Hush theory) evidenced that in such redox species, with an unsymmetrical located redox centre, the electron transfer was not governed by the overall size of the solvated redox species, but rather by the radius of the redox active subunit, which takes preferential orientation towards the surface, thus allowing higher kinetic constants than what classical theory would predict. This vision opens ample opportunities for biredox ILs as electrolytes in electrochemical devices.}, author = {Mourad, Eléonore and Coustan, Laura and Freunberger, Stefan Alexander and Mehdi, Ahmad and Vioux, André and Favier, Frédérique and Fontaine, Olivier}, issn = {0013-4686}, journal = {Electrochimica Acta}, number = {7}, pages = {513--523}, publisher = {Elsevier}, title = {{Biredox ionic liquids: Electrochemical investigation and impact of ion size on electron transfer}}, doi = {10.1016/j.electacta.2016.02.211}, volume = {206}, year = {2016}, } @article{7296, abstract = {Within the scope of developing a multi-physical model describing battery behavior during and after the mechanical load (accelerations, intrusions) of a vehicle’s high voltage battery, an internal short circuit model is of deep interest for a virtual hazard assessment. The internal short resistance and the size of the affected area must be known as a minimum for determining the released heat and, in consequence, the temperatures. The internal short resistance of purpose-built dummy pouch cells, filled with electrolyte-like solvent without conductive salt, has thus been measured in a given short area under various compressive loads. The resistances for different short scenarios obtained are analyzed and described in a mathematical form. Short circuit experiments with dummy cells using an external power source have also been carried out. This set-up allows the measurement of the temperature evolution at a known current and a determination of the actual short resistance. The post-mortem analysis of the samples shows a correlation between the maximum temperatures, released short heat and the separator melt diameter.}, author = {Volck, Theo and Sinz, Wolfgang and Gstrein, Gregor and Breitfuss, Christoph and Heindl, Simon and Steffan, Hermann and Freunberger, Stefan Alexander and Wilkening, Martin and Uitz, Marlena and Fink, Clemens and Geier, Alexander}, issn = {2313-0105}, journal = {Batteries}, number = {2}, publisher = {MDPI AG}, title = {{Method for determination of the internal short resistance and heat evolution at different mechanical loads of a Lithium ion battery cell based on dummy pouch cells}}, doi = {10.3390/batteries2020008}, volume = {2}, year = {2016}, } @article{7297, abstract = {Redox mediators facilitate the oxidation of the highly insulating discharge product in metal–oxygen batteries during recharge and offer opportunities to achieve high reversible capacities. Now a design principle for selecting redox mediators that can recharge the batteries more efficiently is suggested.}, author = {Freunberger, Stefan Alexander}, issn = {2058-7546}, journal = {Nature Energy}, number = {6}, publisher = {Springer Nature}, title = {{Batteries: Charging ahead rationally}}, doi = {10.1038/nenergy.2016.74}, volume = {1}, year = {2016}, } @article{7298, abstract = {Lithium-ion batteries are in widespread use in electric vehicles and hybrid vehicles. Besides features like energy density, cost, lifetime, and recyclability the safety of a battery system is of prime importance. The separator material impacts all these properties and requires therefore an informed selection. The interplay between the mechanical and electrochemical properties as key selection criteria is investigated. Mechanical properties were investigated using tensile and puncture penetration tests at abuse relevant conditions. To investigate the electrochemical performance in terms of effective conductivity a method based on impedance spectroscopy was introduced. This methodology is applied to evaluate ten commercial separators which allows for a trade-off analysis of mechanical versus electrochemical performance. Based on the results, and in combination with other factors, this offers an effective approach to select suitable separators for automotive applications.}, author = {Plaimer, Martin and Breitfuß, Christoph and Sinz, Wolfgang and Heindl, Simon F. and Ellersdorfer, Christian and Steffan, Hermann and Wilkening, Martin and Hennige, Volker and Tatschl, Reinhard and Geier, Alexander and Schramm, Christian and Freunberger, Stefan Alexander}, issn = {0378-7753}, journal = {Journal of Power Sources}, number = {2}, pages = {702--710}, publisher = {Elsevier}, title = {{Evaluating the trade-off between mechanical and electrochemical performance of separators for lithium-ion batteries: Methodology and application}}, doi = {10.1016/j.jpowsour.2015.12.047}, volume = {306}, year = {2016}, } @article{7599, abstract = {Normal leaf margin development is important for leaf morphogenesis and contributes to diverse leaf shapes in higher plants. We here show the crucial roles of an atypical type II phosphatidylinositol 4-kinase, PI4Kγ5, in Arabidopsis leaf margin development. PI4Kγ5 presents a dynamics expression pattern along with leaf development and a T-DNA mutant lacking PI4Kγ5, pi4kγ5–1, presents serrated leaves, which is resulted from the accelerated cell division and increased auxin concentration at serration tips. Studies revealed that PI4Kγ5 interacts with and phosphorylates a membrane-bound NAC transcription factor, ANAC078. Previous studies demonstrated that membrane-bound transcription factors regulate gene transcription by undergoing proteolytic process to translocate into nucleus, and ANAC078 undergoes proteolysis by cleaving off the transmembrane region and carboxyl terminal. Western blot analysis indeed showed that ANAC078 deleting of carboxyl terminal is significantly reduced in pi4kγ5–1, indicating that PI4Kγ5 is important for the cleavage of ANAC078. This is consistent with the subcellular localization observation showing that fluorescence by GFP-ANAC078 is detected at plasma membrane but not nucleus in pi4kγ5–1 mutant and that expression of ANAC078 deleting of carboxyl terminal, driven by PI4Kγ5 promoter, could rescue the leaf serration defects of pi4kγ5–1. Further analysis showed that ANAC078 suppresses the auxin synthesis by directly binding and regulating the expression of auxin synthesis-related genes. These results indicate that PI4Kγ5 interacts with ANAC078 to negatively regulate auxin synthesis and hence influences cell proliferation and leaf development, providing informative clues for the regulation of in situ auxin synthesis and cell division, as well as the cleavage and functional mechanism of membrane-bound transcription factors.}, author = {Tang, Yong and Zhao, Chun-Yan and Tan, Shutang and Xue, Hong-Wei}, issn = {1553-7404}, journal = {PLOS Genetics}, number = {8}, publisher = {Public Library of Science}, title = {{Arabidopsis type II phosphatidylinositol 4-kinase PI4Kγ5 regulates auxin biosynthesis and leaf margin development through interacting with membrane-bound transcription factor ANAC078}}, doi = {10.1371/journal.pgen.1006252}, volume = {12}, year = {2016}, } @misc{9862, author = {Roux, Camille and Fraisse, Christelle and Romiguier, Jonathan and Anciaux, Youann and Galtier, Nicolas and Bierne, Nicolas}, publisher = {Public Library of Science}, title = {{Simulation study to test the robustness of ABC in face of recent times of divergence}}, doi = {10.1371/journal.pbio.2000234.s016}, year = {2016}, } @misc{9863, author = {Roux, Camille and Fraisse, Christelle and Romiguier, Jonathan and Anciaux, Youann and Galtier, Nicolas and Bierne, Nicolas}, publisher = {Public Library of Science}, title = {{Accessions of surveyed individuals, geographic locations and summary statistics}}, doi = {10.1371/journal.pbio.2000234.s017}, year = {2016}, } @misc{9864, abstract = {Viral capsids are structurally constrained by interactions among the amino acids (AAs) of their constituent proteins. Therefore, epistasis is expected to evolve among physically interacting sites and to influence the rates of substitution. To study the evolution of epistasis, we focused on the major structural protein of the ϕX174 phage family by, first, reconstructing the ancestral protein sequences of 18 species using a Bayesian statistical framework. The inferred ancestral reconstruction differed at eight AAs, for a total of 256 possible ancestral haplotypes. For each ancestral haplotype and the extant species, we estimated, in silico, the distribution of free energies and epistasis of the capsid structure. We found that free energy has not significantly increased but epistasis has. We decomposed epistasis up to fifth order and found that higher-order epistasis sometimes compensates pairwise interactions making the free energy seem additive. The dN/dS ratio is low, suggesting strong purifying selection, and that structure is under stabilizing selection. We synthesized phages carrying ancestral haplotypes of the coat protein gene and measured their fitness experimentally. Our findings indicate that stabilizing mutations can have higher fitness, and that fitness optima do not necessarily coincide with energy minima.}, author = {Fernandes Redondo, Rodrigo A and de Vladar, Harold and Włodarski, Tomasz and Bollback, Jonathan P}, publisher = {The Royal Society}, title = {{Data from evolutionary interplay between structure, energy and epistasis in the coat protein of the ϕX174 phage family}}, doi = {10.6084/m9.figshare.4315652.v1}, year = {2016}, } @misc{9866, author = {Zagórski, Marcin P and Burda, Zdzisław and Wacław, Bartłomiej}, publisher = {Public Library of Science}, title = {{ZIP-archived directory containing all data and computer programs}}, doi = {10.1371/journal.pcbi.1005218.s009}, year = {2016}, } @misc{9867, abstract = {In the beginning of our experiment, subjects were asked to read a few pages on their computer screens that would explain the rules of the subsequent game. Here, we provide these instructions, translated from German.}, author = {Hilbe, Christian and Hagel, Kristin and Milinski, Manfred}, publisher = {Public Library of Science}, title = {{Experimental game instructions}}, doi = {10.1371/journal.pone.0163867.s008}, year = {2016}, } @misc{9868, abstract = {The raw data file containing the experimental decisions of all our study subjects.}, author = {Hilbe, Christian and Hagel, Kristin and Milinski, Manfred}, publisher = {Public Library of Science}, title = {{Experimental data}}, doi = {10.1371/journal.pone.0163867.s009}, year = {2016}, } @misc{9869, abstract = {A lower bound on the error of a positional estimator with limited positional information is derived.}, author = {Hillenbrand, Patrick and Gerland, Ulrich and Tkačik, Gašper}, publisher = {Public Library of Science}, title = {{Error bound on an estimator of position}}, doi = {10.1371/journal.pone.0163628.s001}, year = {2016}, } @misc{9870, abstract = {The effect of noise in the input field on an Ising model is approximated. Furthermore, methods to compute positional information in an Ising model by transfer matrices and Monte Carlo sampling are outlined.}, author = {Hillenbrand, Patrick and Gerland, Ulrich and Tkačik, Gašper}, publisher = {Public Library of Science}, title = {{Computation of positional information in an Ising model}}, doi = {10.1371/journal.pone.0163628.s002}, year = {2016}, } @misc{9871, abstract = {The positional information in a discrete morphogen field with Gaussian noise is computed.}, author = {Hillenbrand, Patrick and Gerland, Ulrich and Tkačik, Gašper}, publisher = {Public Library of Science}, title = {{Computation of positional information in a discrete morphogen field}}, doi = {10.1371/journal.pone.0163628.s003}, year = {2016}, } @misc{9873, author = {Boehm, Alex and Arnoldini, Markus and Bergmiller, Tobias and Röösli, Thomas and Bigosch, Colette and Ackermann, Martin}, publisher = {Public Library of Science}, title = {{Quantification of the growth rate reduction as a consequence of age-specific mortality}}, doi = {10.1371/journal.pgen.1005974.s015}, year = {2016}, } @inbook{19990, abstract = {Visualizing molecular localization at high resolution contributes to understanding of their functions and roles in physiological and pathological conditions. Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is a powerful electron microscopy method to study high-resolution two-dimensional distribution of transmembrane proteins and their tightly associated proteins on platinum-carbon replica. During treatment with SDS, unfixed proteins and intracellular organelle are dissolved and integral membrane proteins captured and stabilized by carbon and platinum deposition are denatured, retaining most of their antigenicity, and exposed on exoplasmic and protoplasmic surfaces of lipid monolayers. The exposure of these antigens on the surface of replica facilitates the accessibility of antibodies and therefore provides higher labeling efficiency than those obtained with other immunoelectron microscopy techniques. In this chapter, we describe the protocols of SDS-FRL adapted for mammalian brain samples and an additional procedure for fluorescence-guided electron microscopy for replica immunolabeling.}, author = {Harada, Harumi and Shigemoto, Ryuichi}, booktitle = {Receptor and Ion Channel Detection in the Brain}, isbn = {9781493930630}, issn = {1940-6045}, pages = {233--245}, publisher = {Springer Nature}, title = {{High-Resolution Localization of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)}}, doi = {10.1007/978-1-4939-3064-7_17}, year = {2016}, } @article{1328, abstract = {Hole spins have gained considerable interest in the past few years due to their potential for fast electrically controlled qubits. Here, we study holes confined in Ge hut wires, a so-far unexplored type of nanostructure. Low-temperature magnetotransport measurements reveal a large anisotropy between the in-plane and out-of-plane g-factors of up to 18. Numerical simulations verify that this large anisotropy originates from a confined wave function of heavy-hole character. A light-hole admixture of less than 1% is estimated for the states of lowest energy, leading to a surprisingly large reduction of the out-of-plane g-factors compared with those for pure heavy holes. Given this tiny light-hole contribution, the spin lifetimes are expected to be very long, even in isotopically nonpurified samples.}, author = {Watzinger, Hannes and Kloeffel, Christoph and Vukusic, Lada and Rossell, Marta and Sessi, Violetta and Kukucka, Josip and Kirchschlager, Raimund and Lausecker, Elisabeth and Truhlar, Alisha and Glaser, Martin and Rastelli, Armando and Fuhrer, Andreas and Loss, Daniel and Katsaros, Georgios}, journal = {Nano Letters}, number = {11}, pages = {6879 -- 6885}, publisher = {American Chemical Society}, title = {{Heavy-hole states in germanium hut wires}}, doi = {10.1021/acs.nanolett.6b02715}, volume = {16}, year = {2016}, } @article{1346, abstract = {ATP production requires the establishment of an electrochemical proton gradient across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this proton gradient and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different systems and that ES9 induces inhibition of CME not because of its effect on cellular ATP, but rather due to its protonophore activity that leads to cytoplasm acidification. We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely used to block CME, displays similar properties, thus questioning its use as a specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification dramatically affects the dynamics and recruitment of clathrin and associated adaptors, and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma membrane.}, author = {Dejonghe, Wim and Kuenen, Sabine and Mylle, Evelien and Vasileva, Mina K and Keech, Olivier and Viotti, Corrado and Swerts, Jef and Fendrych, Matyas and Ortiz Morea, Fausto and Mishev, Kiril and Delang, Simon and Scholl, Stefan and Zarza, Xavier and Heilmann, Mareike and Kourelis, Jiorgos and Kasprowicz, Jaroslaw and Nguyen, Le and Drozdzecki, Andrzej and Van Houtte, Isabelle and Szatmári, Anna and Majda, Mateusz and Baisa, Gary and Bednarek, Sebastian and Robert, Stéphanie and Audenaert, Dominique and Testerink, Christa and Munnik, Teun and Van Damme, Daniël and Heilmann, Ingo and Schumacher, Karin and Winne, Johan and Friml, Jirí and Verstreken, Patrik and Russinova, Eugenia}, journal = {Nature Communications}, publisher = {Nature Publishing Group}, title = {{Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification}}, doi = {10.1038/ncomms11710}, volume = {7}, year = {2016}, }