@article{18629, abstract = {We study a three-dimensional Gross-Pitaevskii equation that describes a static impurity in a dipolar Bose-Einstein condensate. Our focus is on the interplay between the shape of the impurity and the anisotropy of the medium manifested in the energy and the density of the system. Without external confinement, properties of the system are derived with basic analytical approaches. For a system in a harmonic trap, the model is investigated numerically, using the split-step Crank-Nicolson method. Our results demonstrate that the impurity self-energy is minimized when its shape more closely aligns with the anisotropic character of the bath; in particular a prolate deformed impurity aligned with the direction of the dipoles has the smallest self-energy for a repulsive impurity. Our work complements studies of impurities in Bose gases with zero-range interactions and paves the way for studies of dipolar polarons with a Gross-Pitaevskii equation.}, author = {Shukla, Neelam and Volosniev, Artem and Armstrong, Jeremy R.}, issn = {2469-9934}, journal = {Physical Review A}, number = {5}, publisher = {American Physical Society}, title = {{Anisotropic potential immersed in a dipolar Bose-Einstein condensate}}, doi = {10.1103/PhysRevA.110.053317}, volume = {110}, year = {2024}, } @article{18645, abstract = {Gene expression during natural and induced reprogramming is controlled by pioneer transcription factors that initiate transcription from closed chromatin. Nr5a2 is a key pioneer factor that regulates zygotic genome activation in totipotent embryos, pluripotency in embryonic stem cells and metabolism in adult tissues, but the mechanism of its pioneer activity remains poorly understood. Here, we present a cryo-electron microscopy structure of human NR5A2 bound to a nucleosome. The structure shows that the conserved carboxy-terminal extension (CTE) loop of the NR5A2 DNA-binding domain competes with a DNA minor groove anchor of the nucleosome and releases entry-exit site DNA. Mutational analysis showed that NR5A2 D159 of the CTE is dispensable for DNA binding but required for stable nucleosome association and persistent DNA ‘unwrapping’. These findings suggest that NR5A2 belongs to an emerging class of pioneer factors that can use DNA minor groove anchor competition to destabilize nucleosomes and facilitate gene expression during reprogramming.}, author = {Kobayashi, Wataru and Sappler, Anna H. and Bollschweiler, Daniel and Kümmecke, Maximilian and Basquin, Jérôme and Arslantas, Eda Nur and Ruangroengkulrith, Siwat and Hornberger, Renate and Duderstadt, Karl and Tachibana, Kikuë}, issn = {1545-9985}, journal = {Nature Structural & Molecular Biology}, pages = {757--766}, publisher = {Springer Nature}, title = {{Nucleosome-bound NR5A2 structure reveals pioneer factor mechanism by DNA minor groove anchor competition}}, doi = {10.1038/s41594-024-01239-0}, volume = {31}, year = {2024}, } @article{18651, abstract = {Embryo axis formation begins with the localized expression of biochemical signals, which organize cell movements and determine cell fate. A quail study finds that tissue contraction and resulting long-range changes in tissue tension restrict the area where these biochemical signals are expressed.}, author = {Hino, Naoya and Santos Fernandes Lasbarrères Camelo, Carolina and Heisenberg, Carl-Philipp J}, issn = {1879-0445}, journal = {Current Biology}, number = {24}, pages = {R1230--R1232}, publisher = {Elsevier}, title = {{Development: Turing mechanics}}, doi = {10.1016/j.cub.2024.10.065}, volume = {34}, year = {2024}, } @article{18652, abstract = {Over the last 70 years, information theory and coding has enabled communication technologies that have had an astounding impact on our lives. This is possible due to the match between encoding/decoding strategies and corresponding channel models. Traditional studies of channels have taken one of two extremes: Shannon-theoretic models are inherently average-case in which channel noise is governed by a memoryless stochastic process, whereas coding-theoretic (referred to as “Hamming”) models take a worst-case, adversarial, view of the noise. However, for several existing and emerging communication systems the Shannon/average-case view may be too optimistic, whereas the Hamming/worstcase view may be too pessimistic. This monograph takes up the challenge of studying adversarial channel models that lie between the Shannon and Hamming extremes.}, author = {Dey, Bikash Kumar and Jaggi, Sidharth and Langberg, Michael and Sarwate, Anand D. and Zhang, Yihan}, issn = {1567-2328}, journal = {Foundations and Trends in Communications and Information Theory}, number = {3-4}, pages = {300--588}, publisher = {Now Publishers}, title = {{Codes for adversaries: Between worst-case and average-case jamming}}, doi = {10.1561/0100000112}, volume = {21}, year = {2024}, } @article{18653, abstract = {Charge sensing is a sensitive technique for probing quantum devices, of particular importance for spin-qubit readout. To achieve good readout sensitivities, the proximity of the charge sensor to the device to be measured is a necessity. However, this proximity also means that the operation of the device affects, in turn, the sensor tuning and ultimately the readout sensitivity. We present an approach for compensating for this crosstalk effect allowing for the gate voltages of the measured device to be swept in a 1-V × 1-V window while maintaining a sensor configuration chosen by a Bayesian optimizer. Our algorithm will hopefully be a major contribution to the suite of fully automated solutions required for the operation of large quantum device architectures.}, author = {Hickie, Joseph and Van Straaten, Barnaby and Fedele, Federico and Jirovec, Daniel and Ballabio, Andrea and Chrastina, Daniel and Isella, Giovanni and Katsaros, Georgios and Ares, Natalia}, issn = {2331-7019}, journal = {Physical Review Applied}, number = {6}, publisher = {American Physical Society}, title = {{Automated long-range compensation of an rf quantum dot sensor}}, doi = {10.1103/PhysRevApplied.22.064026}, volume = {22}, year = {2024}, } @article{18654, abstract = {We compute the rotational anisotropy of the free energy of 𝛼−RuCl3 in an external magnetic field. This quantity, known as the magnetotropic susceptibility, 𝑘, relates to the second derivative of the free energy with respect to the angle of rotation. We have used approximation-free, auxiliary-field quantum Monte Carlo simulations for a realistic model of 𝛼−RuCl3 and optimized the path integral to alleviate the negative sign problem. This allows us to reach temperatures down to 30K—an energy scale below the dominant Kitaev coupling. We demonstrate that the magnetotropic spin susceptibility in this model of 𝛼−RuCl3 displays scaling behavior 𝑘=𝑇⁢𝑓⁡(𝐵/𝑇) at high temperatures. Once the uniform susceptibility departs from the Curie law (i.e., at the energy scale of the exchange interactions), it appears to transition to an emergent scalinglike behavior, characterized by a different function 𝑓 at lower temperatures, stemming from the locality of torque fluctuations. We observe a remarkable numerical match between experiment and simulations and we also find qualitative agreement with the pure Kitaev model. In comparison, for the XXZ Heisenberg Hamiltonian, the scaling 𝑘=𝑇⁢𝑓⁡(𝐵/𝑇) breaks down at a temperature scale where the uniform spin susceptibility deviates from the Curie law and never reemerges at low temperatures.}, author = {Sato, Toshihiro and Ramshaw, B. J. and Modic, Kimberly A and Assaad, Fakher F.}, issn = {2469-9969}, journal = {Physical Review B}, number = {20}, publisher = {American Physical Society}, title = {{Scale-invariant magnetic anisotropy in α-RuCl3: A quantum Monte Carlo study}}, doi = {10.1103/PhysRevB.110.L201114}, volume = {110}, year = {2024}, } @article{18655, abstract = {Let Qd be the d-dimensional binary hypercube. We say that P={v1,…,vk} is an increasing path of length k−1 in Qd, if for every i∈[k−1] the edge vivi+1 is obtained by switching some zero coordinate in vi to a one coordinate in vi+1. Form a random subgraph Qdp by retaining each edge in E(Qd) independently with probability p. We show that there is a phase transition with respect to the length of a longest increasing path around p=ed. Let α be a constant and let p=αd. When αe, whp there is a path of length d−2 in Qdp, and in fact, whether it is of length d−2,d−1, or d depends on whether the all-zero and all-one vertices percolate or not.}, author = {Anastos, Michael and Diskin, Sahar and Elboim, Dor and Krivelevich, Michael}, issn = {1083-589X}, journal = {Electronic Communications in Probability}, publisher = {Duke University Press}, title = {{Climbing up a random subgraph of the hypercube}}, doi = {10.1214/24-ECP639}, volume = {29}, year = {2024}, } @unpublished{18673, abstract = {Motivated by applications to crystalline materials, we generalize the merge tree and the related barcode of a filtered complex to the periodic setting in Euclidean space. They are invariant under isometries, changing bases, and indeed changing lattices. In addition, we prove stability under perturbations and provide an algorithm that under mild geometric conditions typically satisfied by crystalline materials takes O((n+m)logn) time, in which n and m are the numbers of vertices and edges in the quotient complex, respectively. }, author = {Edelsbrunner, Herbert and Heiss, Teresa}, booktitle = {arXiv}, title = {{Merge trees of periodic filtrations}}, doi = {10.48550/arXiv.2408.16575}, year = {2024}, } @phdthesis{18674, abstract = {Mapping the complex and dense arrangement of cells and their connectivity in brain tissue requires volumetric imaging at nanoscale spatial resolution. While light microscopy excels at visualizing specific molecules and individual cells, achieving dense, synapse-level circuit reconstruction has not been possible with any light microscopy technique. Thus, the goal of my work was to develop image and data analysis pipelines for brain tissue visualization and reconstruction with light microscopy. To achieve dense circuit reconstruction with single-synapse resolution, I developed both conventional and deep-learning-based synapse detection algorithms, as well as connectivity analysis pipelines that integrate synapse detection with volumetric segmentation of brain tissue.}, author = {Lyudchik, Julia}, isbn = { 978-3-99078-051-0}, issn = {2663-337X}, pages = {217}, publisher = {Institute of Science and Technology Austria}, title = {{Image analysis for brain tissue reconstruction with super-resolution light microscopy}}, doi = {10.15479/at:ista:18674}, year = {2024}, } @article{18704, abstract = {Clathrin-mediated endocytosis is the main pathway used by eukaryotic cells to take up extracellular material, but the dominant physical mechanisms driving this process are still elusive. Recently, several high-resolution imaging techniques have been used on different cell lines to measure the geometrical properties of clathrin-coated pits over their whole lifetime. Here, we first show that the combination of all datasets with the recently introduced cooperative curvature model defines a consensus pathway, which is characterized by a flat-to-curved transition at finite area, followed by linear growth and subsequent saturation of curvature. We then apply an energetic model for the composite of the plasma membrane and clathrin coat to this consensus pathway to show that the dominant mechanism for invagination could be coat stiffening, which might originate from cooperative interactions between the different clathrin molecules and progressively drives the system toward its intrinsic curvature. Our theory predicts that two length scales determine the invagination pathway, namely the patch size at which the flat-to-curved transition occurs and the final pit radius.}, author = {Frey, Felix F and Schwarz, Ulrich S.}, issn = {2470-0053}, journal = {Physical Review E}, number = {6}, publisher = {American Physical Society}, title = {{Coat stiffening can explain invagination of clathrin-coated membranes}}, doi = {10.1103/PhysRevE.110.064403}, volume = {110}, year = {2024}, } @article{18708, abstract = {This study investigates the response of Indian summer monsoon (ISM) precipitation to CO2 removal, with a specific focus on regional and subseasonal variations. Following CO2 removal, monsoon circulation weakens throughout the summer owing to the reduced large-scale meridional temperature gradient around India. Weakened monsoon circulation decreases the local-scale thermodynamic stability within India, following monsoon-onset periods. While the frequency of synoptic-scale ISM low-pressure systems (LPSs) decreases overall, the lower thermodynamic stability causes the LPSs to form and resultantly shift west and south from their typical paths, last longer and move more quickly zonally during August and September. Changes in these rain-producing processes induce distinct regional (Western Ghats, south-central-east India, and Tamil Nadu) and subseasonal precipitation responses. Also, extreme precipitation exhibits similar patterns, but is more strongly affected by changes in LPS. Our results suggest that reliable future projections of regional hydroclimate change require a more accurate understanding of multi-scale precipitation processes.}, author = {Paik, Seungmok and Kim, Daehyun and An, Soon Il and Oh, Hyoeun and Shin, Jongsoo and Goswami, Bidyut B and Min, Seung Ki and Mondal, Sanjit Kumar}, issn = {2397-3722}, journal = {npj Climate and Atmospheric Science}, publisher = {Springer Nature}, title = {{Exploring causes of distinct regional and subseasonal Indian summer monsoon precipitation responses to CO2 removal}}, doi = {10.1038/s41612-024-00858-0}, volume = {7}, year = {2024}, } @article{18709, abstract = {We measure the mass distribution of main-sequence (MS) companions to hot subdwarf B stars (sdBs) in post-common envelope binaries (PCEBs). We carried out a spectroscopic survey of 14 eclipsing systems ("HW Vir binaries") with orbital periods of 3.8 < Porb < 12 hr, resulting in a well-understood selection function and a near-complete sample of HW Vir binaries with G < 16. We constrain companion masses from the radial velocity curves of the sdB stars. The companion mass distribution peaks at MMS ≈ 0.15 M⊙ and drops off at MMS > 0.2 M⊙, with only two systems hosting companions above the fully convective limit. There is no correlation between Porb and MMS within the sample. A similar drop-off in the companion mass distribution of white dwarf (WD) + MS PCEBs has been attributed to disrupted magnetic braking (MB) below the fully convective limit. We compare the sdB companion mass distribution to predictions of binary evolution simulations with a range of MB laws. Because sdBs have short lifetimes compared to WDs, explaining the lack of higher-mass MS companions to sdBs with disrupted MB requires MB to be boosted by a factor of 20–100 relative to MB laws inferred from the rotation evolution of single stars. We speculate that such boosting may be a result of irradiation-driven enhancement of the MS stars' winds. An alternative possibility is that common envelope evolution favors low-mass companions in short-period orbits, but the existence of massive WD companions to sdBs with similar periods disfavors this scenario.}, author = {Blomberg, Lisa and El-Badry, Kareem and Breivik, Katelyn and Caiazzo, Ilaria and Nagarajan, Pranav and Rodriguez, Antonio and Van Roestel, Jan and Vanderbosch, Zachary P. and Yamaguchi, Natsuko}, issn = {0004-6280}, journal = {Publications of the Astronomical Society of the Pacific}, number = {12}, publisher = {IOP Publishing}, title = {{The companion mass distribution of post common envelope hot subdwarf binaries: Evidence for boosted and disrupted magnetic braking?}}, doi = {10.1088/1538-3873/ad94a2}, volume = {136}, year = {2024}, } @misc{18716, abstract = {Data for publication 10.1039/d4cp03727h}, author = {Hrast, Mateja}, publisher = {Zenodo}, title = {{Data for: Ab initio Auger spectrum of the ultrafast dissociating 2p3/2−1σ* resonance in HCl}}, doi = {10.5281/ZENODO.13833474}, year = {2024}, } @inproceedings{18755, abstract = {A universalthresholdizer (UT), constructed from a threshold fully homomorphic encryption by Boneh et. al , Crypto 2018, is a general framework for universally thresholdizing many cryptographic schemes. However, their framework is insufficient to construct strongly secure threshold schemes, such as threshold signatures and threshold public-key encryption, etc. In this paper, we strengthen the security definition for a universal thresholdizer and propose a scheme which satisfies our stronger security notion. Our UT scheme is an improvement of Boneh et. al ’s construction at the level of threshold fully homomorphic encryption using a key homomorphic pseudorandom function. We apply our strongly secure UT scheme to construct strongly secure threshold signatures and threshold public-key encryption.}, author = {Ebrahimi, Ehsan and Yadav, Anshu}, booktitle = {30th International Conference on the Theory and Application of Cryptology and Information Security}, isbn = {9789819608904}, issn = {1611-3349}, location = {Kolkata, India}, pages = {207--239}, publisher = {Springer Nature}, title = {{Strongly secure universal thresholdizer}}, doi = {10.1007/978-981-96-0891-1_7}, volume = {15486}, year = {2024}, } @inproceedings{18756, abstract = {The evasive LWE assumption, proposed by Wee [Eurocrypt’22 Wee] for constructing a lattice-based optimal broadcast encryption, has shown to be a powerful assumption, adopted by subsequent works to construct advanced primitives ranging from ABE variants to obfuscation for null circuits. However, a closer look reveals significant differences among the precise assumption statements involved in different works, leading to the fundamental question of how these assumptions compare to each other. In this work, we initiate a more systematic study on evasive LWE assumptions: (i) Based on the standard LWE assumption, we construct simple counterexamples against three private-coin evasive LWE variants, used in [Crypto’22 Tsabary, Asiacrypt’22 VWW, Crypto’23 ARYY] respectively, showing that these assumptions are unlikely to hold. (ii) Based on existing evasive LWE variants and our counterexamples, we propose and define three classes of plausible evasive LWE assumptions, suitably capturing all existing variants for which we are not aware of non-obfuscation-based counterexamples. (iii) We show that under our assumption formulations, the security proofs of [Asiacrypt’22 VWW] and [Crypto’23 ARYY] can be recovered, and we reason why the security proof of [Crypto’22 Tsabary] is also plausibly repairable using an appropriate evasive LWE assumption.}, author = {Brzuska, Chris and Ünal, Akin and Woo, Ivy K.Y.}, booktitle = {30th International Conference on the Theory and Application of Cryptology and Information Security}, isbn = {9789819608935}, issn = {1611-3349}, location = {Kolkata, India}, pages = {418--449}, publisher = {Springer Nature}, title = {{Evasive LWE assumptions: Definitions, classes, and counterexamples}}, doi = {10.1007/978-981-96-0894-2_14}, volume = {15487}, year = {2024}, } @article{18757, abstract = {Segmentation is a critical data processing step in many applications of cryo-electron tomography. Downstream analyses, such as subtomogram averaging, are often based on segmentation results, and are thus critically dependent on the availability of open-source software for accurate as well as high-throughput tomogram segmentation. There is a need for more user-friendly, flexible, and comprehensive segmentation software that offers an insightful overview of all steps involved in preparing automated segmentations. Here, we present Ais: a dedicated tomogram segmentation package that is geared towards both high performance and accessibility, available on GitHub. In this report, we demonstrate two common processing steps that can be greatly accelerated with Ais: particle picking for subtomogram averaging, and generating many-feature segmentations of cellular architecture based on in situ tomography data. Featuring comprehensive annotation, segmentation, and rendering functionality, as well as an open repository for trained models at aiscryoet.org, we hope that Ais will help accelerate research and dissemination of data involving cryoET.}, author = {Last, Mart G.F. and Abendstein, Leoni and Voortman, Lenard M. and Sharp, Thomas H.}, issn = {2050-084X}, journal = {eLife}, publisher = {eLife Sciences Publications}, title = {{Streamlining segmentation of cryo-electron tomography datasets with Ais}}, doi = {10.7554/eLife.98552}, volume = {13}, year = {2024}, } @inproceedings{18758, abstract = {MaxCut is a classical NP-complete problem and a crucial building block in many combinatorial algorithms. The famous Edwards-Erdős bound states that any connected graph on n vertices with m edges contains a cut of size at least m/2+(n-1)/4. Crowston, Jones and Mnich [Algorithmica, 2015] showed that the MaxCut problem on simple connected graphs admits an FPT algorithm, where the parameter k is the difference between the desired cut size c and the lower bound given by the Edwards-Erdős bound. This was later improved by Etscheid and Mnich [Algorithmica, 2017] to run in parameterized linear time, i.e., f(k)⋅ O(m). We improve upon this result in two ways: Firstly, we extend the algorithm to work also for multigraphs (alternatively, graphs with positive integer weights). Secondly, we change the parameter; instead of the difference to the Edwards-Erdős bound, we use the difference to the Poljak-Turzík bound. The Poljak-Turzík bound states that any weighted graph G has a cut of size at least (w(G))/2+(w_MSF(G))/4, where w(G) denotes the total weight of G, and w_MSF(G) denotes the weight of its minimum spanning forest. In connected simple graphs the two bounds are equivalent, but for multigraphs the Poljak-Turzík bound can be larger and thus yield a smaller parameter k. Our algorithm also runs in parameterized linear time, i.e., f(k)⋅ O(m+n).}, author = {Lill, Jonas and Petrova, Kalina H and Weber, Simon}, booktitle = {19th International Symposium on Parameterized and Exact Computation}, isbn = {9783959773539}, issn = {1868-8969}, location = {Egham, United Kingdom}, publisher = {Schloss Dagstuhl - Leibniz-Zentrum für Informatik}, title = {{Linear-time MaxCut in multigraphs parameterized above the Poljak-Turzík bound}}, doi = {10.4230/LIPIcs.IPEC.2024.2}, volume = {321}, year = {2024}, } @article{18760, abstract = {With the remarkable sensitivity and resolution of JWST in the infrared, measuring rest-optical kinematics of galaxies at z > 5 has become possible for the first time. This study pilots a new method for measuring galaxy dynamics for highly multiplexed, unbiased samples by combining FRESCO NIRCam grism spectroscopy and JADES medium-band imaging. Here we present one of the first JWST kinematic measurements for a galaxy at z > 5. We find a significant velocity gradient, which, if interpreted as rotation, yields Vrot = 305 ± 70 km s−1, and we hence refer to this galaxy as Twister-z5. With a rest-frame optical effective radius of re = 2.25 kpc, the high rotation velocity in this galaxy is not due to a compact size, as may be expected in the early Universe, but rather to a high total mass, (math formula). This is a factor of roughly 10× higher than the stellar mass within re. We also observe that the radial Hα equivalent width profile and the specific star formation rate map from resolved stellar population modeling are centrally depressed by a factor of ∼1.5 from the center to re. Combined with the morphology of the line-emitting gas in comparison to the continuum, this centrally suppressed star formation is consistent with a star-forming disk surrounding a bulge growing inside out. While large, rapidly rotating disks are common to z ∼ 2, the existence of one after only 1 Gyr of cosmic time, shown for the first time in ionized gas, adds to the growing evidence that some galaxies matured earlier than expected in the history of the Universe.}, author = {Nelson, Erica and Brammer, Gabriel and Giménez-Arteaga, Clara and Oesch, Pascal A. and Naidu, Rohan P. and Übler, Hannah and Matharu, Jasleen and Shapley, Alice E. and Whitaker, Katherine E. and Wisnioski, Emily and Förster Schreiber, Natascha M. and Smit, Renske and Van Dokkum, Pieter and Chisholm, John and Endsley, Ryan and Hartley, Abigail I. and Gibson, Justus and Giovinazzo, Emma and Illingworth, Garth and Labbe, Ivo and Maseda, Michael V. and Matthee, Jorryt J and Covelo Paz, Alba and Price, Sedona H. and Reddy, Naveen A. and Shivaei, Irene and Weibel, Andrea and Wuyts, Stijn and Xiao, Mengyuan and Alberts, Stacey and Baker, William M. and Bunker, Andrew J. and Cameron, Alex J. and Charlot, Stephane and Eisenstein, Daniel J. and De Graaff, Anna and Ji, Zhiyuan and Johnson, Benjamin D. and Jones, Gareth C. and Maiolino, Roberto and Robertson, Brant and Sandles, Lester and Suess, Katherine A. and Tacchella, Sandro and Williams, Christina C. and Witstok, Joris}, issn = {2041-8213}, journal = {Astrophysical Journal Letters}, number = {2}, publisher = {IOP Publishing}, title = {{Ionized gas kinematics with FRESCO: An extended, massive, rapidly rotating galaxy at z = 5.4}}, doi = {10.3847/2041-8213/ad7b17}, volume = {976}, year = {2024}, } @article{18761, abstract = {Termites, together with cockroaches, belong to the Blattodea. They possess an XX/XY sex determination system which has evolved from an XX/X0 system present in other Blattodean species, such as cockroaches and wood roaches. Little is currently known about the sex chromosomes of termites, their gene content, or their evolution. We here investigate the X chromosome of multiple termite species and compare them with the X chromosome of cockroaches using genomic and transcriptomic data. We find that the X chromosome of the termite Macrotermes natalensis is large and differentiated showing hall marks of sex chromosome evolution such as dosage compensation, while this does not seem to be the case in the other two termite species investigated here where sex chromosomes may be evolutionary younger. Furthermore, the X chromosome in M. natalensis is different from the X chromosome found in the cockroach Blattella germanica indicating that sex chromosome turn-over events may have happened during termite evolution.}, author = {Fraser, Roxanne and Moraa, Ruth and Djolai, Annika and Meisenheimer, Nils and Laube, Sophie and Vicoso, Beatriz and Huylmans, Ann K}, issn = {1759-6653}, journal = {Genome Biology and Evolution}, number = {12}, publisher = {Oxford University Press}, title = {{Evidence for a novel X chromosome in termites}}, doi = {10.1093/gbe/evae265}, volume = {16}, year = {2024}, } @article{18762, abstract = {Consider the random variable $\mathrm{Tr}( f_1(W)A_1\dots f_k(W)A_k)$ where $W$ is an $N\times N$ Hermitian Wigner matrix, $k\in\mathbb{N}$, and choose (possibly $N$-dependent) regular functions $f_1,\dots, f_k$ as well as bounded deterministic matrices $A_1,\dots,A_k$. We give a functional central limit theorem showing that the fluctuations around the expectation are Gaussian. Moreover, we determine the limiting covariance structure and give explicit error bounds in terms of the scaling of $f_1,\dots,f_k$ and the number of traceless matrices among $A_1,\dots,A_k$, thus extending the results of [Cipolloni, Erdős, Schröder 2023] to products of arbitrary length $k\geq2$. As an application, we consider the fluctuation of $\mathrm{Tr}(\mathrm{e}^{\mathrm{i} tW}A_1\mathrm{e}^{-\mathrm{i} tW}A_2)$ around its thermal value $\mathrm{Tr}(A_1)\mathrm{Tr}(A_2)$ when $t$ is large and give an explicit formula for the variance.}, author = {Reker, Jana}, issn = {1083-6489}, journal = {Electronic Journal of Probability}, publisher = {Institute of Mathematical Statistics}, title = {{Multi-point functional central limit theorem for Wigner matrices}}, doi = {10.1214/24-EJP1247}, volume = {29}, year = {2024}, }