[{"date_created":"2024-02-12T11:42:18Z","acknowledgement":"We thank Pere Rosselló for his contributions to the initial modeling of the presented sensing technique. This work was supported by Institute of Science and Technology Austria, and\r\nthe European Research Council under Grant No. 101087907 (ERC CoG QuHAMP).","article_type":"original","volume":6,"type":"journal_article","month":"02","supplementarymaterial":"no","ddc":["530"],"file":[{"success":1,"date_updated":"2024-02-12T11:46:50Z","access_level":"open_access","date_created":"2024-02-12T11:46:50Z","file_name":"2024_PhysicalRevResearch_Agafonova.pdf","creator":"dernst","content_type":"application/pdf","relation":"main_file","file_size":1437167,"file_id":"14981","checksum":"3a39ebffb24c1cc1dd0b547a726dc52d"}],"department":[{"_id":"OnHo"}],"arxiv":1,"language":[{"iso":"eng"}],"has_accepted_license":"1","corr_author":"1","OA_place":"publisher","file_date_updated":"2024-02-12T11:46:50Z","scopus_import":"1","publication_identifier":{"eissn":["2643-1564"]},"publisher":"American Physical Society","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"APC_amount":"2933,65 EUR","date_updated":"2026-09-17T09:13:08Z","das_tickbox":"0","title":"Zigzag optical cavity for sensing and controlling torsional motion","DOAJ_listed":"1","oa":1,"day":"05","fulldoi":"https://doi.org/10.1103/physrevresearch.6.013141","date_published":"2024-02-05T00:00:00Z","project":[{"name":"A quantum hybrid of atoms and milligram-scale pendulums: towards gravitational quantum mechanics","grant_number":"101087907","_id":"bdb2a702-d553-11ed-ba76-f12e3e5a3bc6"}],"oa_version":"Published Version","intvolume":"         6","abstract":[{"text":"Precision sensing and manipulation of milligram-scale mechanical oscillators has attracted growing interest in the fields of table-top explorations of gravity and tests of quantum mechanics at macroscopic scales. Torsional oscillators present an opportunity in this regard due to their remarked isolation from environmental noise. For torsional motion, an effective employment of optical cavities to enhance optomechanical interactions—as already established for linear oscillators—so far faced certain challenges. Here, we propose a concept for sensing and manipulating torsional motion, where exclusively the torsional rotations of a pendulum are mapped onto the path length of a single two-mirror optical cavity. The concept inherently alleviates many limitations of previous approaches. A proof-of-principle experiment is conducted with a rigidly controlled pendulum to explore the sensing aspects of the concept and to identify practical limitations in a potential state-of-the art setup. Based on this study, we anticipate development of precision torque sensors utilizing torsional pendulums that can support sensitivities below 10−19Nm/√Hz, while the motion of the pendulums are dominated by quantum radiation pressure noise at sub-microwatts of incoming laser power. These developments will provide horizons for experiments at the interface of quantum mechanics and gravity.","lang":"eng"}],"publication_status":"published","author":[{"last_name":"Agafonova","full_name":"Agafonova, Sofya","id":"09501ff6-dca7-11ea-a8ae-b3e0b9166e80","first_name":"Sofya","orcid":"0000-0003-0582-2946"},{"first_name":"Umang","last_name":"Mishra","full_name":"Mishra, Umang","id":"4328fa4c-f128-11eb-9611-c107b0fe4d51"},{"id":"2E054C4C-F248-11E8-B48F-1D18A9856A87","full_name":"Diorico, Fritz R","last_name":"Diorico","orcid":"0000-0002-4947-8924","first_name":"Fritz R"},{"last_name":"Hosten","full_name":"Hosten, Onur","id":"4C02D85E-F248-11E8-B48F-1D18A9856A87","first_name":"Onur","orcid":"0000-0002-2031-204X"}],"issue":"1","researchdata_availability":"no","quality_controlled":"1","doi":"10.1103/physrevresearch.6.013141","year":"2024","OA_type":"gold","_id":"14980","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"Yes","external_id":{"arxiv":["2306.12804"]},"citation":{"ama":"Agafonova S, Mishra U, Diorico FR, Hosten O. Zigzag optical cavity for sensing and controlling torsional motion. <i>Physical Review Research</i>. 2024;6(1). doi:<a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">10.1103/physrevresearch.6.013141</a>","chicago":"Agafonova, Sofia, Umang Mishra, Fritz R Diorico, and Onur Hosten. “Zigzag Optical Cavity for Sensing and Controlling Torsional Motion.” <i>Physical Review Research</i>. American Physical Society, 2024. <a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">https://doi.org/10.1103/physrevresearch.6.013141</a>.","mla":"Agafonova, Sofia, et al. “Zigzag Optical Cavity for Sensing and Controlling Torsional Motion.” <i>Physical Review Research</i>, vol. 6, no. 1, 013141, American Physical Society, 2024, doi:<a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">10.1103/physrevresearch.6.013141</a>.","short":"S. Agafonova, U. Mishra, F.R. Diorico, O. Hosten, Physical Review Research 6 (2024).","apa":"Agafonova, S., Mishra, U., Diorico, F. R., &#38; Hosten, O. (2024). Zigzag optical cavity for sensing and controlling torsional motion. <i>Physical Review Research</i>. American Physical Society. <a href=\"https://doi.org/10.1103/physrevresearch.6.013141\">https://doi.org/10.1103/physrevresearch.6.013141</a>","ieee":"S. Agafonova, U. Mishra, F. R. Diorico, and O. Hosten, “Zigzag optical cavity for sensing and controlling torsional motion,” <i>Physical Review Research</i>, vol. 6, no. 1. American Physical Society, 2024.","ista":"Agafonova S, Mishra U, Diorico FR, Hosten O. 2024. Zigzag optical cavity for sensing and controlling torsional motion. Physical Review Research. 6(1), 013141."},"article_number":"013141","publication":"Physical Review Research"},{"day":"01","oa":1,"das_tickbox":"0","title":"NMR and single-molecule FRET insights into fast protein motions and their relation to function","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-17T09:44:33Z","publication_identifier":{"eissn":["1936-1238"],"issn":["1936-122X"]},"publisher":"Annual Reviews","has_accepted_license":"1","corr_author":"1","scopus_import":"1","OA_place":"publisher","file_date_updated":"2025-01-27T13:44:59Z","file":[{"content_type":"application/pdf","relation":"main_file","file_size":3025589,"file_id":"18911","checksum":"c90861542ae3f9147939030d5bafed3c","date_updated":"2025-01-27T13:44:59Z","success":1,"date_created":"2025-01-27T13:44:59Z","access_level":"open_access","file_name":"2024_AnnualReviews_Schanda.pdf","creator":"dernst"}],"department":[{"_id":"PaSc"}],"language":[{"iso":"eng"}],"page":"247-273","pmid":1,"date_created":"2025-01-27T13:40:34Z","article_type":"original","volume":53,"acknowledgement":"G.H. is the incumbent of the Hilda Pomeraniec Memorial Professorial Chair. He has been partially funded by the European Research Council under the European Union's Horizon 2020 research and innovation program (grant 742637, SMALLOSTERY), by National Science Foundation–US-Israel Binational Science Foundation grant 2021700, and by an Israel Science Foundation Breakthrough grant (1924/22). P.S. acknowledges funding from the Austrian Science Fund (project “AlloSpace,” I05812) and intramural funding from the Institute of Science and Technology Austria.","type":"journal_article","ddc":["570"],"month":"07","supplementarymaterial":"no","publication":"Annual Review of Biophysics","article_processing_charge":"No","external_id":{"pmid":["38346243"],"isi":["001278237500012"]},"citation":{"ama":"Schanda P, Haran G. NMR and single-molecule FRET insights into fast protein motions and their relation to function. <i>Annual Review of Biophysics</i>. 2024;53:247-273. doi:<a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">10.1146/annurev-biophys-070323-022428</a>","apa":"Schanda, P., &#38; Haran, G. (2024). NMR and single-molecule FRET insights into fast protein motions and their relation to function. <i>Annual Review of Biophysics</i>. Annual Reviews. <a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">https://doi.org/10.1146/annurev-biophys-070323-022428</a>","ieee":"P. Schanda and G. Haran, “NMR and single-molecule FRET insights into fast protein motions and their relation to function,” <i>Annual Review of Biophysics</i>, vol. 53. Annual Reviews, pp. 247–273, 2024.","ista":"Schanda P, Haran G. 2024. NMR and single-molecule FRET insights into fast protein motions and their relation to function. Annual Review of Biophysics. 53, 247–273.","mla":"Schanda, Paul, and Gilad Haran. “NMR and Single-Molecule FRET Insights into Fast Protein Motions and Their Relation to Function.” <i>Annual Review of Biophysics</i>, vol. 53, Annual Reviews, 2024, pp. 247–73, doi:<a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">10.1146/annurev-biophys-070323-022428</a>.","chicago":"Schanda, Paul, and Gilad Haran. “NMR and Single-Molecule FRET Insights into Fast Protein Motions and Their Relation to Function.” <i>Annual Review of Biophysics</i>. Annual Reviews, 2024. <a href=\"https://doi.org/10.1146/annurev-biophys-070323-022428\">https://doi.org/10.1146/annurev-biophys-070323-022428</a>.","short":"P. Schanda, G. Haran, Annual Review of Biophysics 53 (2024) 247–273."},"OA_type":"hybrid","_id":"18910","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","year":"2024","quality_controlled":"1","doi":"10.1146/annurev-biophys-070323-022428","researchdata_availability":"no","intvolume":"        53","abstract":[{"text":"Proteins often undergo large-scale conformational transitions, in which secondary and tertiary structure elements (loops, helices, and domains) change their structures or their positions with respect to each other. Simple considerations suggest that such dynamics should be relatively fast, but the functional cycles of many proteins are often relatively slow. Sophisticated experimental methods are starting to tackle this dichotomy and shed light on the contribution of large-scale conformational dynamics to protein function. In this review, we focus on the contribution of single-molecule Förster resonance energy transfer and nuclear magnetic resonance (NMR) spectroscopies to the study of conformational dynamics. We briefly describe the state of the art in each of these techniques and then point out their similarities and differences, as well as the relative strengths and weaknesses of each. Several case studies, in which the connection between fast conformational dynamics and slower function has been demonstrated, are then introduced and discussed. These examples include both enzymes and large protein machines, some of which have been studied by both NMR and fluorescence spectroscopies.","lang":"eng"}],"author":[{"id":"7B541462-FAF6-11E9-A490-E8DFE5697425","full_name":"Schanda, Paul","last_name":"Schanda","orcid":"0000-0002-9350-7606","first_name":"Paul"},{"first_name":"Gilad","last_name":"Haran","full_name":"Haran, Gilad"}],"publication_status":"published","fulldoi":"https://doi.org/10.1146/annurev-biophys-070323-022428","oa_version":"Published Version","project":[{"grant_number":"I05812","name":"AlloSpace. The emergence and mechanisms of allostery","_id":"eb9c82eb-77a9-11ec-83b8-aadd536561cf"}],"isi":1,"date_published":"2024-07-01T00:00:00Z"},{"_id":"15117","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2024","publication":"Science","citation":{"short":"D.H. Vandael, P.M. Jonas, Science 383 (2024) eadg6757.","mla":"Vandael, David H., and Peter M. Jonas. “Structure, Biophysics, and Circuit Function of a ‘Giant’ Cortical Presynaptic Terminal.” <i>Science</i>, vol. 383, no. 6687, AAAS, 2024, p. eadg6757, doi:<a href=\"https://doi.org/10.1126/science.adg6757\">10.1126/science.adg6757</a>.","chicago":"Vandael, David H, and Peter M Jonas. “Structure, Biophysics, and Circuit Function of a ‘Giant’ Cortical Presynaptic Terminal.” <i>Science</i>. AAAS, 2024. <a href=\"https://doi.org/10.1126/science.adg6757\">https://doi.org/10.1126/science.adg6757</a>.","ista":"Vandael DH, Jonas PM. 2024. Structure, biophysics, and circuit function of a ‘giant’ cortical presynaptic terminal. Science. 383(6687), eadg6757.","ieee":"D. H. Vandael and P. M. Jonas, “Structure, biophysics, and circuit function of a ‘giant’ cortical presynaptic terminal,” <i>Science</i>, vol. 383, no. 6687. AAAS, p. eadg6757, 2024.","apa":"Vandael, D. H., &#38; Jonas, P. M. (2024). Structure, biophysics, and circuit function of a “giant” cortical presynaptic terminal. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adg6757\">https://doi.org/10.1126/science.adg6757</a>","ama":"Vandael DH, Jonas PM. Structure, biophysics, and circuit function of a “giant” cortical presynaptic terminal. <i>Science</i>. 2024;383(6687):eadg6757. doi:<a href=\"https://doi.org/10.1126/science.adg6757\">10.1126/science.adg6757</a>"},"article_processing_charge":"No","external_id":{"isi":["001216996700015"],"pmid":["38452088"]},"issue":"6687","publication_status":"published","author":[{"full_name":"Vandael, David H","id":"3AE48E0A-F248-11E8-B48F-1D18A9856A87","last_name":"Vandael","orcid":"0000-0001-7577-1676","first_name":"David H"},{"first_name":"Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M"}],"abstract":[{"text":"The hippocampal mossy fiber synapse, formed between axons of dentate gyrus granule cells and dendrites of CA3 pyramidal neurons, is a key synapse in the trisynaptic circuitry of the hippocampus. Because of its comparatively large size, this synapse is accessible to direct presynaptic recording, allowing a rigorous investigation of the biophysical mechanisms of synaptic transmission and plasticity. Furthermore, because of its placement in the very center of the hippocampal memory circuit, this synapse seems to be critically involved in several higher network functions, such as learning, memory, pattern separation, and pattern completion. Recent work based on new technologies in both nanoanatomy and nanophysiology, including presynaptic patch-clamp recording, paired recording, super-resolution light microscopy, and freeze-fracture and “flash-and-freeze” electron microscopy, has provided new insights into the structure, biophysics, and network function of this intriguing synapse. This brings us one step closer to answering a fundamental question in neuroscience: how basic synaptic properties shape higher network computations.","lang":"eng"}],"intvolume":"       383","oa_version":"None","fulldoi":"https://doi.org/10.1126/science.adg6757","project":[{"_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","grant_number":"692692"},{"name":"Synaptic communication in neuronal microcircuits","grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","grant_number":"P36232","name":"Mechanisms of GABA release in hippocampal circuits"}],"isi":1,"date_published":"2024-03-08T00:00:00Z","quality_controlled":"1","doi":"10.1126/science.adg6757","researchdata_availability":"no","title":"Structure, biophysics, and circuit function of a \"giant\" cortical presynaptic terminal","das_tickbox":"0","date_updated":"2026-09-17T09:49:03Z","day":"08","department":[{"_id":"PeJo"}],"language":[{"iso":"eng"}],"type":"journal_article","supplementarymaterial":"no","month":"03","page":"eadg6757","date_created":"2024-03-17T23:00:57Z","pmid":1,"article_type":"review","volume":383,"acknowledgement":"We thank previous students, postdocs, and collaborators, particularly J. Geiger, and (in alphabetical order) H. Alle, J. Bischofberger, C. Borges-Merjane, D. Engel, M. Frotscher, S. Hallermann, M. Heckmann, S. Jamrichova, O. Kim, L. Li, K. Lichter, P. Lin, J. Lübke, Y. Okamoto, C. Pawlu, C. Schmidt-Hieber, N. Spruston, and N. Vyleta for their outstanding experimental contributions. We also thank P. Castillo, J. Geiger, T. Sakaba, S. Siegert, T. Vogels, and J. Watson for critically reading the manuscript, E. Kralli-Beller for text editing, and J. Malikovic and L. Slomianka for useful discussions. We apologize that, due to space constraints, not all relevant papers could be cited.\r\nThis project was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement 692692, AdG “GIANTSYN”) and the Fonds zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein Award; P 36232-B, stand-alone grant), both to P.J.","publisher":"AAAS","ec_funded":1,"publication_identifier":{"eissn":["1095-9203"]},"corr_author":"1","scopus_import":"1"},{"year":"2024","_id":"18058","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","citation":{"ieee":"J. Watson, S. Arroyo-Urea, and J. García-Nafría, “DNA Cloning,” in <i>Handbook of Molecular Biotechnology</i>, 1st ed., D. Liu, Ed. Boca Raton: CRC Press, 2024, pp. 66–72.","apa":"Watson, J., Arroyo-Urea, S., &#38; García-Nafría, J. (2024). DNA Cloning. In D. Liu (Ed.), <i>Handbook of Molecular Biotechnology</i> (1st ed., pp. 66–72). Boca Raton: CRC Press. <a href=\"https://doi.org/10.1201/9781003055211-8\">https://doi.org/10.1201/9781003055211-8</a>","ista":"Watson J, Arroyo-Urea S, García-Nafría J. 2024.DNA Cloning. In: Handbook of Molecular Biotechnology. , 66–72.","mla":"Watson, Jake, et al. “DNA Cloning.” <i>Handbook of Molecular Biotechnology</i>, edited by Dongyou Liu, 1st ed., CRC Press, 2024, pp. 66–72, doi:<a href=\"https://doi.org/10.1201/9781003055211-8\">10.1201/9781003055211-8</a>.","chicago":"Watson, Jake, Sandra Arroyo-Urea, and Javier García-Nafría. “DNA Cloning.” In <i>Handbook of Molecular Biotechnology</i>, edited by Dongyou Liu, 1st ed., 66–72. Boca Raton: CRC Press, 2024. <a href=\"https://doi.org/10.1201/9781003055211-8\">https://doi.org/10.1201/9781003055211-8</a>.","short":"J. Watson, S. Arroyo-Urea, J. García-Nafría, in:, D. Liu (Ed.), Handbook of Molecular Biotechnology, 1st ed., CRC Press, Boca Raton, 2024, pp. 66–72.","ama":"Watson J, Arroyo-Urea S, García-Nafría J. DNA Cloning. In: Liu D, ed. <i>Handbook of Molecular Biotechnology</i>. 1st ed. Boca Raton: CRC Press; 2024:66-72. doi:<a href=\"https://doi.org/10.1201/9781003055211-8\">10.1201/9781003055211-8</a>"},"article_processing_charge":"No","publication":"Handbook of Molecular Biotechnology","date_published":"2024-09-05T00:00:00Z","fulldoi":"https://doi.org/10.1201/9781003055211-8","oa_version":"None","publication_status":"published","author":[{"orcid":"0000-0002-8698-3823","first_name":"Jake","full_name":"Watson, Jake","id":"63836096-4690-11EA-BD4E-32803DDC885E","last_name":"Watson"},{"full_name":"Arroyo-Urea, Sandra","last_name":"Arroyo-Urea","first_name":"Sandra"},{"first_name":"Javier","last_name":"García-Nafría","full_name":"García-Nafría, Javier"}],"abstract":[{"text":"DNA cloning is a core technique in biomedical and biotechnological research and is used to assemble and modify DNA fragments at will. While DNA cloning has traditionally relied on restriction enzymes, recent homology-based methods offer improved protocols together with seamless and directional assembly of desired products, overcoming the main disadvantages of restriction enzyme DNA cloning. This chapter provides a historical perspective on DNA cloning, presents a detailed discussion on state-of-the-art in vitro and in vivo homology-based methodologies, covering the basics of how to perform all major plasmid modifications (sub-cloning, site-directed mutagenesis, insertions, and deletions), and gives examples of how to apply these techniques for complex DNA cloning projects.","lang":"eng"}],"researchdata_availability":"no","quality_controlled":"1","doi":"10.1201/9781003055211-8","date_updated":"2026-09-17T09:58:21Z","place":"Boca Raton","title":"DNA Cloning","das_tickbox":"0","editor":[{"first_name":"Dongyou","last_name":"Liu","full_name":"Liu, Dongyou"}],"day":"05","month":"09","type":"book_chapter","supplementarymaterial":"no","edition":"1","date_created":"2024-09-11T10:40:36Z","page":"66-72","department":[{"_id":"PeJo"}],"language":[{"iso":"eng"}],"scopus_import":"1","publisher":"CRC Press","publication_identifier":{"eisbn":["9781003055211"]}},{"publication_status":"published","author":[{"full_name":"Lauwereyns, Jan","last_name":"Lauwereyns","first_name":"Jan"},{"first_name":"Jeffrey","last_name":"Bajramovic","full_name":"Bajramovic, Jeffrey"},{"first_name":"Bettina","last_name":"Bert","full_name":"Bert, Bettina"},{"first_name":"Samuel","full_name":"Camenzind, Samuel","last_name":"Camenzind"},{"last_name":"De Kock","full_name":"De Kock, Joery","first_name":"Joery"},{"full_name":"Elezović, Alisa","last_name":"Elezović","first_name":"Alisa"},{"first_name":"Sevilay","last_name":"Erden","full_name":"Erden, Sevilay"},{"last_name":"Gonzalez-Uarquin","full_name":"Gonzalez-Uarquin, Fernando","first_name":"Fernando"},{"full_name":"Ulman, Yesim Isil","last_name":"Ulman","first_name":"Yesim Isil"},{"last_name":"Hoffmann","full_name":"Hoffmann, Orsolya Ivett","first_name":"Orsolya Ivett"},{"first_name":"Maria","full_name":"Kitsara, Maria","last_name":"Kitsara"},{"first_name":"Nikolaos","last_name":"Kostomitsopoulos","full_name":"Kostomitsopoulos, Nikolaos"},{"first_name":"Winfried","last_name":"Neuhaus","full_name":"Neuhaus, Winfried"},{"last_name":"Petit-Demouliere","full_name":"Petit-Demouliere, Benoit","first_name":"Benoit"},{"first_name":"Simone","last_name":"Pollo","full_name":"Pollo, Simone"},{"first_name":"Brígida","last_name":"Riso","full_name":"Riso, Brígida"},{"full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","last_name":"Schober","first_name":"Sophie"},{"last_name":"Sotiropoulos","full_name":"Sotiropoulos, Athanassia","first_name":"Athanassia"},{"first_name":"Aurélie","full_name":"Thomas, Aurélie","last_name":"Thomas"},{"first_name":"Augusto","full_name":"Vitale, Augusto","last_name":"Vitale"},{"first_name":"Doris","last_name":"Wilflingseder","full_name":"Wilflingseder, Doris"},{"last_name":"Ahluwalia","full_name":"Ahluwalia, Arti","first_name":"Arti"}],"intvolume":"        53","abstract":[{"text":"Many scientific breakthroughs have depended on animal research, yet the ethical concerns surrounding the use of animals in experimentation have long prompted discussions about humane treatment and responsible scientific practice. First articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction, and Refinement have gained widespread recognition as basic guidelines for animal research. Over time, the 3Rs have transcended the research community, influencing policy decisions, animal welfare advocacy and public perception of animal experimentation. Despite their broad acceptance, interpretations of the 3Rs vary substantially, shaping statutory frameworks at various levels, with both technical and practical impacts.","lang":"eng"}],"date_published":"2024-12-01T00:00:00Z","fulldoi":"https://doi.org/10.1038/s41684-024-01476-2","isi":1,"oa_version":"Published Version","doi":"10.1038/s41684-024-01476-2","quality_controlled":"1","researchdata_availability":"no","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"18587","OA_type":"hybrid","year":"2024","publication":"Lab Animal","citation":{"short":"J. Lauwereyns, J. Bajramovic, B. Bert, S. Camenzind, J. De Kock, A. Elezović, S. Erden, F. Gonzalez-Uarquin, Y.I. Ulman, O.I. Hoffmann, M. Kitsara, N. Kostomitsopoulos, W. Neuhaus, B. Petit-Demouliere, S. Pollo, B. Riso, S. Schober, A. Sotiropoulos, A. Thomas, A. Vitale, D. Wilflingseder, A. Ahluwalia, Lab Animal 53 (2024) 347–350.","mla":"Lauwereyns, Jan, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>, vol. 53, Springer Nature, 2024, pp. 347–50, doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>.","chicago":"Lauwereyns, Jan, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery De Kock, Alisa Elezović, Sevilay Erden, et al. “Toward a Common Interpretation of the 3Rs Principles in Animal Research.” <i>Lab Animal</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>.","apa":"Lauwereyns, J., Bajramovic, J., Bert, B., Camenzind, S., De Kock, J., Elezović, A., … Ahluwalia, A. (2024). Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41684-024-01476-2\">https://doi.org/10.1038/s41684-024-01476-2</a>","ieee":"J. Lauwereyns <i>et al.</i>, “Toward a common interpretation of the 3Rs principles in animal research,” <i>Lab Animal</i>, vol. 53. Springer Nature, pp. 347–350, 2024.","ista":"Lauwereyns J, Bajramovic J, Bert B, Camenzind S, De Kock J, Elezović A, Erden S, Gonzalez-Uarquin F, Ulman YI, Hoffmann OI, Kitsara M, Kostomitsopoulos N, Neuhaus W, Petit-Demouliere B, Pollo S, Riso B, Schober S, Sotiropoulos A, Thomas A, Vitale A, Wilflingseder D, Ahluwalia A. 2024. Toward a common interpretation of the 3Rs principles in animal research. Lab Animal. 53, 347–350.","ama":"Lauwereyns J, Bajramovic J, Bert B, et al. Toward a common interpretation of the 3Rs principles in animal research. <i>Lab Animal</i>. 2024;53:347-350. doi:<a href=\"https://doi.org/10.1038/s41684-024-01476-2\">10.1038/s41684-024-01476-2</a>"},"external_id":{"isi":["001355264100001"],"pmid":["39548348"]},"article_processing_charge":"No","language":[{"iso":"eng"}],"department":[{"_id":"PreCl"}],"file":[{"file_name":"2024_LabAnimal_Lauwereyns.pdf","creator":"dernst","success":1,"date_updated":"2024-12-03T14:07:04Z","date_created":"2024-12-03T14:07:04Z","access_level":"open_access","file_id":"18614","file_size":967252,"checksum":"67fc140f761581a291591f075e49b88d","content_type":"application/pdf","relation":"main_file"}],"month":"12","type":"journal_article","ddc":["570"],"supplementarymaterial":"no","acknowledgement":"This publication is based upon work from the Ethics Crossover Group within the COST Action IMPROVE (“3Rs concepts to improve the quality of biomedical science”), CA21139, supported by COST (European Cooperation in Science and Technology). We acknowledge the input and advice from Dr. Susanna Louhimies.","article_type":"letter_note","volume":53,"date_created":"2024-11-24T23:01:49Z","page":"347-350","pmid":1,"publisher":"Springer Nature","publication_identifier":{"eissn":["1548-4475"],"issn":["0093-7355"]},"file_date_updated":"2024-12-03T14:07:04Z","OA_place":"publisher","scopus_import":"1","has_accepted_license":"1","title":"Toward a common interpretation of the 3Rs principles in animal research","das_tickbox":"0","date_updated":"2026-09-17T10:29:32Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"day":"01","oa":1},{"abstract":[{"text":"Light-driven rotary molecular motors are among the most promising classes of responsive molecular machines and take advantage of their intrinsic chirality which governs unidirectional rotation. As a consequence of their dynamic function, they receive considerable interest in the areas of supramolecular chemistry, asymmetric catalysis and responsive materials. Among the emerging classes of responsive photochromic molecules, multistate first-generation molecular motors driven by benign visible light remain unexplored, which limits the exploitation of the full potential of these mechanical light-powered systems. Herein, we describe a series of all-visible-light-driven first-generation molecular motors based on the salicylidene Schiff base functionality. Remarkable redshifts up to 100 nm in absorption are achieved compared to conventional first-generation motor structures. Taking advantage of all-visible-light-driven multistate motor scaffolds, adaptive behaviour is found as well, and potential application in multistate photoluminescence is demonstrated. These functional visible-light-responsive motors will likely stimulate the design and synthesis of more sophisticated nanomachinery with a myriad of future applications in powering dynamic systems.","lang":"eng"}],"intvolume":"        15","publication_status":"published","author":[{"first_name":"Sven","last_name":"Van Vliet","full_name":"Van Vliet, Sven"},{"id":"639f0526-27c9-11ee-95a6-966cd7f102d8","full_name":"Sheng, Jinyu","last_name":"Sheng","first_name":"Jinyu"},{"last_name":"Stindt","full_name":"Stindt, Charlotte N.","first_name":"Charlotte N."},{"full_name":"Feringa, Ben L.","last_name":"Feringa","first_name":"Ben L."}],"isi":1,"fulldoi":"https://doi.org/10.1038/s41467-024-50587-4","oa_version":"Published Version","date_published":"2024-07-31T00:00:00Z","doi":"10.1038/s41467-024-50587-4","quality_controlled":"1","researchdata_availability":"yes","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"17409","year":"2024","article_number":"6461","publication":"Nature Communications","external_id":{"pmid":["39085193"],"isi":["001282384300020"]},"article_processing_charge":"Yes","citation":{"chicago":"Van Vliet, Sven, Jinyu Sheng, Charlotte N. Stindt, and Ben L. Feringa. “All-Visible-Light-Driven Salicylidene Schiff-Base-Functionalized Artificial Molecular Motors.” <i>Nature Communications</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41467-024-50587-4\">https://doi.org/10.1038/s41467-024-50587-4</a>.","mla":"Van Vliet, Sven, et al. “All-Visible-Light-Driven Salicylidene Schiff-Base-Functionalized Artificial Molecular Motors.” <i>Nature Communications</i>, vol. 15, 6461, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s41467-024-50587-4\">10.1038/s41467-024-50587-4</a>.","short":"S. Van Vliet, J. Sheng, C.N. Stindt, B.L. Feringa, Nature Communications 15 (2024).","ieee":"S. Van Vliet, J. Sheng, C. N. Stindt, and B. L. Feringa, “All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors,” <i>Nature Communications</i>, vol. 15. Springer Nature, 2024.","apa":"Van Vliet, S., Sheng, J., Stindt, C. N., &#38; Feringa, B. L. (2024). All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-024-50587-4\">https://doi.org/10.1038/s41467-024-50587-4</a>","ista":"Van Vliet S, Sheng J, Stindt CN, Feringa BL. 2024. All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors. Nature Communications. 15, 6461.","ama":"Van Vliet S, Sheng J, Stindt CN, Feringa BL. All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors. <i>Nature Communications</i>. 2024;15. doi:<a href=\"https://doi.org/10.1038/s41467-024-50587-4\">10.1038/s41467-024-50587-4</a>"},"dataavailabilitystatement":"Data that support the findings of this study are available within the Supplementary Information. All data are available from the corresponding author upon request. The XYZ coordinates of the DFT-optimized structures are available as source data. Source data are provided with this paper.","file":[{"content_type":"application/pdf","relation":"main_file","file_size":1355696,"file_id":"17418","checksum":"4aca2ea30fe529947cb2f4c7fc2af2df","date_updated":"2024-08-12T08:23:10Z","success":1,"access_level":"open_access","date_created":"2024-08-12T08:23:10Z","file_name":"2024_NatureComm_vanVliet.pdf","creator":"dernst"}],"language":[{"iso":"eng"}],"department":[{"_id":"RaKl"}],"acknowledgement":"Financial support from the Netherlands Organization for Scientific Research (NWO-CW), the Dutch Ministry of Education, Culture and Science (Gravitation program No.024.001.035), the China Scholarship Council (CSC PhD Fellowship No.201808330459 to J. S.). We thank Dr. Youxin Fu, Dr. Alexander Ryabchun, Dr. Wojciech Danowski, Dr. Jianyu Zhang and Yahan Shan from University of Groningen for their help to this project and fruitful discussions.","article_type":"original","volume":15,"date_created":"2024-08-11T22:01:11Z","pmid":1,"month":"07","ddc":["540"],"supplementarymaterial":"yes","type":"journal_article","publication_identifier":{"eissn":["2041-1723"]},"publisher":"Springer Nature","has_accepted_license":"1","scopus_import":"1","file_date_updated":"2024-08-12T08:23:10Z","das_tickbox":"1","title":"All-visible-light-driven salicylidene schiff-base-functionalized artificial molecular motors","DOAJ_listed":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-17T10:46:04Z","day":"31","oa":1},{"file":[{"checksum":"7ecb0892051f6ed8f6a6f25baa47543a","file_id":"17107","file_size":2363206,"relation":"main_file","content_type":"application/pdf","creator":"dernst","file_name":"2024_AngChemieInt_Sheng.pdf","date_created":"2024-06-03T10:33:17Z","access_level":"open_access","success":1,"date_updated":"2024-06-03T10:33:17Z"}],"dataavailabilitystatement":"The data that support the findings of this study are available in the supplementary material of this article.","department":[{"_id":"RaKl"}],"language":[{"iso":"eng"}],"date_created":"2024-06-03T09:00:01Z","pmid":1,"volume":63,"acknowledgement":"This work was supported from the following sources: China Scholarship Council (CSC PhD Fellowship No. 201808330459 to J.S.), the Ministry of Education, Culture and Science of the Netherlands (Gravitation Program No. 024.001.035 to BLF), Financial support from The Netherlands Organization for Scientific Research (NWO-CW), the European Research Council (ERC; advanced Grant No. 694345 to B.L.F.). PRIN (SHERPA 2020 No. H45F21003430001) and PRIN (HySTAR 2022 No. H53D23004720006) and Lombardy Region for “Enhancing Photosynthesis” grant (2021-2023 No. H45F21002830007). W.D. is grateful for financial support from Marie Skłodowska-Curie Actions (Individual Fellowship No. 101027639). P.C. is grateful for the financial support provided by the PRELUDIUM grant from the National Science Center Poland (Reg. No: 2023/49/N/ST5/01864).","article_type":"original","month":"03","ddc":["540"],"supplementarymaterial":"yes","type":"journal_article","publication_identifier":{"eissn":["1521-3773"],"issn":["1433-7851"]},"publisher":"Wiley","has_accepted_license":"1","scopus_import":"1","file_date_updated":"2024-06-03T10:33:17Z","das_tickbox":"1","title":"Orthogonal photoswitching in a porous organic framework","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-17T10:32:51Z","day":"26","oa":1,"abstract":[{"text":"The development of photoresponsive systems with non-invasive orthogonal control by distinct wavelengths of light is still in its infancy. In particular, the design of photochemically triggered-orthogonal systems integrated into solid materials that enable multiple dynamic control over their properties remains a longstanding challenge. Here, we report the orthogonal and reversible control of two types of photoswitches in an integrated solid porous framework, that is, visible-light responsive o-fluoroazobenzene and nitro-spiropyran motifs. The properties of the constructed material can be selectively controlled by different wavelengths of light thus generating four distinct states providing a basis for dynamic multifunctional materials. Solid-state NMR spectroscopy demonstrated the selective transformation of the azobenzene switch in the bulk, which in turn modulates N2 and CO2 adsorption.","lang":"eng"}],"intvolume":"        63","author":[{"first_name":"Jinyu","id":"639f0526-27c9-11ee-95a6-966cd7f102d8","full_name":"Sheng, Jinyu","last_name":"Sheng"},{"last_name":"Perego","full_name":"Perego, Jacopo","first_name":"Jacopo"},{"full_name":"Bracco, Silvia","last_name":"Bracco","first_name":"Silvia"},{"full_name":"Cieciórski, Piotr","last_name":"Cieciórski","first_name":"Piotr"},{"first_name":"Wojciech","last_name":"Danowski","full_name":"Danowski, Wojciech"},{"full_name":"Comotti, Angiolina","last_name":"Comotti","first_name":"Angiolina"},{"first_name":"Ben L.","full_name":"Feringa, Ben L.","last_name":"Feringa"}],"issue":"23","publication_status":"published","fulldoi":"https://doi.org/10.1002/anie.202404878","oa_version":"Published Version","date_published":"2024-03-26T00:00:00Z","quality_controlled":"1","doi":"10.1002/anie.202404878","researchdata_availability":"yes","_id":"17105","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","year":"2024","article_number":"e202404878","publication":"Angewandte Chemie International Edition","article_processing_charge":"Yes (in subscription journal)","external_id":{"pmid":["38530132"]},"citation":{"ama":"Sheng J, Perego J, Bracco S, et al. Orthogonal photoswitching in a porous organic framework. <i>Angewandte Chemie International Edition</i>. 2024;63(23). doi:<a href=\"https://doi.org/10.1002/anie.202404878\">10.1002/anie.202404878</a>","chicago":"Sheng, Jinyu, Jacopo Perego, Silvia Bracco, Piotr Cieciórski, Wojciech Danowski, Angiolina Comotti, and Ben L. Feringa. “Orthogonal Photoswitching in a Porous Organic Framework.” <i>Angewandte Chemie International Edition</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/anie.202404878\">https://doi.org/10.1002/anie.202404878</a>.","mla":"Sheng, Jinyu, et al. “Orthogonal Photoswitching in a Porous Organic Framework.” <i>Angewandte Chemie International Edition</i>, vol. 63, no. 23, e202404878, Wiley, 2024, doi:<a href=\"https://doi.org/10.1002/anie.202404878\">10.1002/anie.202404878</a>.","short":"J. Sheng, J. Perego, S. Bracco, P. Cieciórski, W. Danowski, A. Comotti, B.L. Feringa, Angewandte Chemie International Edition 63 (2024).","apa":"Sheng, J., Perego, J., Bracco, S., Cieciórski, P., Danowski, W., Comotti, A., &#38; Feringa, B. L. (2024). Orthogonal photoswitching in a porous organic framework. <i>Angewandte Chemie International Edition</i>. Wiley. <a href=\"https://doi.org/10.1002/anie.202404878\">https://doi.org/10.1002/anie.202404878</a>","ieee":"J. Sheng <i>et al.</i>, “Orthogonal photoswitching in a porous organic framework,” <i>Angewandte Chemie International Edition</i>, vol. 63, no. 23. Wiley, 2024.","ista":"Sheng J, Perego J, Bracco S, Cieciórski P, Danowski W, Comotti A, Feringa BL. 2024. Orthogonal photoswitching in a porous organic framework. Angewandte Chemie International Edition. 63(23), e202404878."}},{"file":[{"success":1,"date_updated":"2025-01-09T09:38:51Z","date_created":"2025-01-09T09:38:51Z","access_level":"open_access","file_name":"2024_Aggregate_Shan.pdf","creator":"dernst","content_type":"application/pdf","relation":"main_file","file_size":2299084,"file_id":"18804","checksum":"1e79dc81d0edf0b441ef661a1890bbf5"}],"language":[{"iso":"eng"}],"department":[{"_id":"RaKl"}],"article_type":"original","volume":5,"acknowledgement":"This work was supported by the National Natural Science Foundation of China (grant nos. 22220102004, 22025503), Shanghai Municipal Science and Technology Major Project (grant no. 2018SHZDZX03), the Innovation Program of Shanghai Municipal Education Commission (2023ZKZD40), the Fundamental Research Funds for the Central Universities, the Program of Introducing Talents of Discipline to Universities (grant no. B16017), Science and Technology Commission of Shanghai Municipality (grant no. 21JC1401700), and the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study (grant no. SN-ZJU-SIAS-006), China Scholarship Council (CSC PhD Fellowship No. 202006745016 to Yahan Shan). The authors gratefully acknowledge financial support from the Dutch Ministry of Education, Culture and Science (gravitation program no. 024.001.035 to Ben L. Feringa). The authors thank Dr. Youxin Fu and Dr. Alexander Ryabchun for the help with fluorescence quantum yield measurement, Cristina Nitu for the help with photoisomerzation quantum yield measurement, Prof. Wesley R. Browne for the help with fluorescence lifetime measurement, and Dr. Jianyu Zhang for fruitful discussion and revising the manuscript.","date_created":"2024-05-26T22:00:58Z","supplementarymaterial":"yes","month":"10","type":"journal_article","ddc":["540"],"publication_identifier":{"eissn":["2692-4560"],"issn":["2766-8541"]},"publisher":"Wiley","has_accepted_license":"1","file_date_updated":"2025-01-09T09:38:51Z","OA_place":"publisher","scopus_import":"1","das_tickbox":"0","title":"Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-17T11:01:40Z","day":"01","oa":1,"intvolume":"         5","abstract":[{"text":"Photoisomerization and photoluminescence are two distinct energy dissipation pathways in light-driven molecular motors. The photoisomerization properties of discrete molecular motors have been well established in solution, but their photoluminescent properties have been rarely reported—especially in aggregates. Here, it is shown that an overcrowded alkene-based molecular motor exhibits distinct dynamic properties in solution and aggregate states, for example, gel and solid states. Despite the poor emissive properties of molecular motors in solution, a bright emission is observed in the aggregate states, including in gel and the crystalline solid. The emission wavelength is highly dependent on the nature of the supramolecular packing and order in the aggregates. As a result, the fluorescent color can be readily tuned reversibly via mechanical grinding and vapor fuming, which provides a new platform for developing multi-stimuli functional materials.","lang":"eng"}],"publication_status":"published","issue":"5","author":[{"first_name":"Yahan","last_name":"Shan","full_name":"Shan, Yahan"},{"full_name":"Sheng, Jinyu","id":"639f0526-27c9-11ee-95a6-966cd7f102d8","last_name":"Sheng","first_name":"Jinyu"},{"first_name":"Qi","full_name":"Zhang, Qi","last_name":"Zhang"},{"last_name":"Stuart","full_name":"Stuart, Marc C.A.","first_name":"Marc C.A."},{"last_name":"Qu","full_name":"Qu, Da Hui","first_name":"Da Hui"},{"first_name":"Ben L.","full_name":"Feringa, Ben L.","last_name":"Feringa"}],"fulldoi":"https://doi.org/10.1002/agt2.584","oa_version":"Published Version","date_published":"2024-10-01T00:00:00Z","doi":"10.1002/agt2.584","quality_controlled":"1","researchdata_availability":"no","OA_type":"gold","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"17054","year":"2024","article_number":"e584","publication":"Aggregate","article_processing_charge":"Yes","citation":{"apa":"Shan, Y., Sheng, J., Zhang, Q., Stuart, M. C. A., Qu, D. H., &#38; Feringa, B. L. (2024). Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates. <i>Aggregate</i>. Wiley. <a href=\"https://doi.org/10.1002/agt2.584\">https://doi.org/10.1002/agt2.584</a>","ieee":"Y. Shan, J. Sheng, Q. Zhang, M. C. A. Stuart, D. H. Qu, and B. L. Feringa, “Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates,” <i>Aggregate</i>, vol. 5, no. 5. Wiley, 2024.","ista":"Shan Y, Sheng J, Zhang Q, Stuart MCA, Qu DH, Feringa BL. 2024. Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates. Aggregate. 5(5), e584.","short":"Y. Shan, J. Sheng, Q. Zhang, M.C.A. Stuart, D.H. Qu, B.L. Feringa, Aggregate 5 (2024).","mla":"Shan, Yahan, et al. “Multi-State Photoluminescent Properties of an Overcrowded Alkene-Based Molecular Motor in Aggregates.” <i>Aggregate</i>, vol. 5, no. 5, e584, Wiley, 2024, doi:<a href=\"https://doi.org/10.1002/agt2.584\">10.1002/agt2.584</a>.","chicago":"Shan, Yahan, Jinyu Sheng, Qi Zhang, Marc C.A. Stuart, Da Hui Qu, and Ben L. Feringa. “Multi-State Photoluminescent Properties of an Overcrowded Alkene-Based Molecular Motor in Aggregates.” <i>Aggregate</i>. Wiley, 2024. <a href=\"https://doi.org/10.1002/agt2.584\">https://doi.org/10.1002/agt2.584</a>.","ama":"Shan Y, Sheng J, Zhang Q, Stuart MCA, Qu DH, Feringa BL. Multi-state photoluminescent properties of an overcrowded alkene-based molecular motor in aggregates. <i>Aggregate</i>. 2024;5(5). doi:<a href=\"https://doi.org/10.1002/agt2.584\">10.1002/agt2.584</a>"}},{"citation":{"ama":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, et al. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. 2024;52(6):326-333. doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>","short":"M. Kitsara, M. Smajlhodžić-Deljo, L. Gurbeta Pokvic, B. Bert, N. Bubalo, S. Erden, N.H. Franco, G. Chirico, J. Gómez Raja, F. Gonzalez-Uarquin, A. Lang, N. Linklater, S. Mojsova, I.A.S. Olsson, I. Sandvig, A. Schaffert, M. Schmit, S. Schober, B. Sevastre, D. Wilflingseder, A. Ahluwalia, W. Neuhaus, Alternatives to Laboratory Animals 52 (2024) 326–333.","mla":"Kitsara, Maria, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6, SAGE Publications, 2024, pp. 326–33, doi:<a href=\"https://doi.org/10.1177/02611929241286024\">10.1177/02611929241286024</a>.","chicago":"Kitsara, Maria, Merima Smajlhodžić-Deljo, Lejla Gurbeta Pokvic, Bettina Bert, Nataliia Bubalo, Sevilay Erden, Nuno Henrique Franco, et al. “Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE).” <i>Alternatives to Laboratory Animals</i>. SAGE Publications, 2024. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>.","ieee":"M. Kitsara <i>et al.</i>, “Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE),” <i>Alternatives to Laboratory Animals</i>, vol. 52, no. 6. SAGE Publications, pp. 326–333, 2024.","apa":"Kitsara, M., Smajlhodžić-Deljo, M., Gurbeta Pokvic, L., Bert, B., Bubalo, N., Erden, S., … Neuhaus, W. (2024). Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). <i>Alternatives to Laboratory Animals</i>. SAGE Publications. <a href=\"https://doi.org/10.1177/02611929241286024\">https://doi.org/10.1177/02611929241286024</a>","ista":"Kitsara M, Smajlhodžić-Deljo M, Gurbeta Pokvic L, Bert B, Bubalo N, Erden S, Franco NH, Chirico G, Gómez Raja J, Gonzalez-Uarquin F, Lang A, Linklater N, Mojsova S, Olsson IAS, Sandvig I, Schaffert A, Schmit M, Schober S, Sevastre B, Wilflingseder D, Ahluwalia A, Neuhaus W. 2024. Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE). Alternatives to Laboratory Animals. 52(6), 326–333."},"article_processing_charge":"No","external_id":{"isi":["001348633700007"],"pmid":["39333027"]},"publication":"Alternatives to Laboratory Animals","year":"2024","_id":"18310","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","OA_type":"closed access","researchdata_availability":"no","quality_controlled":"1","doi":"10.1177/02611929241286024","fulldoi":"https://doi.org/10.1177/02611929241286024","isi":1,"date_published":"2024-11-01T00:00:00Z","oa_version":"None","publication_status":"published","author":[{"first_name":"Maria","last_name":"Kitsara","full_name":"Kitsara, Maria"},{"full_name":"Smajlhodžić-Deljo, Merima","last_name":"Smajlhodžić-Deljo","first_name":"Merima"},{"full_name":"Gurbeta Pokvic, Lejla","last_name":"Gurbeta Pokvic","first_name":"Lejla"},{"last_name":"Bert","full_name":"Bert, Bettina","first_name":"Bettina"},{"full_name":"Bubalo, Nataliia","last_name":"Bubalo","first_name":"Nataliia"},{"full_name":"Erden, Sevilay","last_name":"Erden","first_name":"Sevilay"},{"first_name":"Nuno Henrique","full_name":"Franco, Nuno Henrique","last_name":"Franco"},{"full_name":"Chirico, Giuseppe","last_name":"Chirico","first_name":"Giuseppe"},{"full_name":"Gómez Raja, Jonathan","last_name":"Gómez Raja","first_name":"Jonathan"},{"full_name":"Gonzalez-Uarquin, Fernando","last_name":"Gonzalez-Uarquin","first_name":"Fernando"},{"first_name":"Annemarie","last_name":"Lang","full_name":"Lang, Annemarie"},{"first_name":"Nicole","last_name":"Linklater","full_name":"Linklater, Nicole"},{"last_name":"Mojsova","full_name":"Mojsova, Sandra","first_name":"Sandra"},{"full_name":"Olsson, I. Anna S.","last_name":"Olsson","first_name":"I. Anna S."},{"last_name":"Sandvig","full_name":"Sandvig, Ioanna","first_name":"Ioanna"},{"first_name":"Alexandra","last_name":"Schaffert","full_name":"Schaffert, Alexandra"},{"last_name":"Schmit","full_name":"Schmit, Marthe","first_name":"Marthe"},{"first_name":"Sophie","full_name":"Schober, Sophie","id":"80b0a0ef-4b9f-11ec-b119-8d9d94c4a1d8","last_name":"Schober"},{"first_name":"Bogdan","last_name":"Sevastre","full_name":"Sevastre, Bogdan"},{"full_name":"Wilflingseder, Doris","last_name":"Wilflingseder","first_name":"Doris"},{"last_name":"Ahluwalia","full_name":"Ahluwalia, Arti","first_name":"Arti"},{"last_name":"Neuhaus","full_name":"Neuhaus, Winfried","first_name":"Winfried"}],"issue":"6","intvolume":"        52","day":"01","date_updated":"2026-09-17T10:30:08Z","title":"Introducing the COST action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)","das_tickbox":"0","scopus_import":"1","publisher":"SAGE Publications","publication_identifier":{"eissn":["2632-3559"],"issn":["0261-1929"]},"supplementarymaterial":"no","type":"journal_article","month":"11","pmid":1,"date_created":"2024-10-13T22:01:51Z","page":"326-333","volume":52,"article_type":"original","department":[{"_id":"PreCl"}],"language":[{"iso":"eng"}]},{"citation":{"ama":"Bossmann L, Leopold NK, Mitrouskas DJ, Petrat SP. A note on the binding energy for Bosons in the mean-field limit. <i>Journal of Statistical Physics</i>. 2024;191(4). doi:<a href=\"https://doi.org/10.1007/s10955-024-03260-5\">10.1007/s10955-024-03260-5</a>","short":"L. Bossmann, N.K. Leopold, D.J. Mitrouskas, S.P. Petrat, Journal of Statistical Physics 191 (2024).","chicago":"Bossmann, Lea, Nikolai K Leopold, David Johannes Mitrouskas, and Sören P Petrat. “A Note on the Binding Energy for Bosons in the Mean-Field Limit.” <i>Journal of Statistical Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1007/s10955-024-03260-5\">https://doi.org/10.1007/s10955-024-03260-5</a>.","mla":"Bossmann, Lea, et al. “A Note on the Binding Energy for Bosons in the Mean-Field Limit.” <i>Journal of Statistical Physics</i>, vol. 191, no. 4, 48, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1007/s10955-024-03260-5\">10.1007/s10955-024-03260-5</a>.","ieee":"L. Bossmann, N. K. Leopold, D. J. Mitrouskas, and S. P. Petrat, “A note on the binding energy for Bosons in the mean-field limit,” <i>Journal of Statistical Physics</i>, vol. 191, no. 4. Springer Nature, 2024.","apa":"Bossmann, L., Leopold, N. K., Mitrouskas, D. J., &#38; Petrat, S. P. (2024). A note on the binding energy for Bosons in the mean-field limit. <i>Journal of Statistical Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s10955-024-03260-5\">https://doi.org/10.1007/s10955-024-03260-5</a>","ista":"Bossmann L, Leopold NK, Mitrouskas DJ, Petrat SP. 2024. A note on the binding energy for Bosons in the mean-field limit. Journal of Statistical Physics. 191(4), 48."},"external_id":{"arxiv":["2307.13115"],"isi":["001197663100002"]},"article_processing_charge":"Yes (via OA deal)","publication":"Journal of Statistical Physics","article_number":"48","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"15318","researchdata_availability":"not applicable","doi":"10.1007/s10955-024-03260-5","quality_controlled":"1","fulldoi":"https://doi.org/10.1007/s10955-024-03260-5","date_published":"2024-04-06T00:00:00Z","oa_version":"Published Version","isi":1,"publication_status":"published","issue":"4","author":[{"id":"A2E3BCBE-5FCC-11E9-AA4B-76F3E5697425","full_name":"Bossmann, Lea","last_name":"Bossmann","orcid":"0000-0002-6854-1343","first_name":"Lea"},{"full_name":"Leopold, Nikolai K","id":"4BC40BEC-F248-11E8-B48F-1D18A9856A87","last_name":"Leopold","orcid":"0000-0002-0495-6822","first_name":"Nikolai K"},{"last_name":"Mitrouskas","full_name":"Mitrouskas, David Johannes","id":"cbddacee-2b11-11eb-a02e-a2e14d04e52d","first_name":"David Johannes"},{"last_name":"Petrat","full_name":"Petrat, Sören P","id":"40AC02DC-F248-11E8-B48F-1D18A9856A87","first_name":"Sören P","orcid":"0000-0002-9166-5889"}],"intvolume":"       191","abstract":[{"lang":"eng","text":"We consider a gas of N weakly interacting bosons in the ground state. Such gases exhibit Bose–Einstein condensation. The binding energy is defined as the energy it takes to remove one particle from the gas. In this article, we prove an asymptotic expansion for the binding energy, and compute the first orders explicitly for the homogeneous gas. Our result addresses in particular a conjecture by Nam (Lett Math Phys 108(1):141–159, 2018), and provides an asymptotic expansion of the ionization energy of bosonic atoms."}],"oa":1,"day":"06","date_updated":"2026-09-17T11:05:18Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"A note on the binding energy for Bosons in the mean-field limit","das_tickbox":"1","file_date_updated":"2024-04-16T11:09:37Z","scopus_import":"1","has_accepted_license":"1","publisher":"Springer Nature","publication_identifier":{"eissn":["1572-9613"],"issn":["0022-4715"]},"type":"journal_article","month":"04","supplementarymaterial":"no","ddc":["510"],"acknowledgement":"It is a pleasure to thank Phan Thành Nam for helpful discussions on bosonic atoms. L.B. was supported by the German Research Foundation within the Munich Center of Quantum Science and Technology (EXC 2111). N.L. gratefully acknowledges support from the Swiss National Science Foundation through the NCCR SwissMap and funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant agreement No 101024712. S.P. acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project number 512258249.\r\nOpen Access funding enabled and organized by Projekt DEAL.","volume":191,"article_type":"original","date_created":"2024-04-14T22:01:02Z","language":[{"iso":"eng"}],"arxiv":1,"department":[{"_id":"RoSe"}],"file":[{"content_type":"application/pdf","relation":"main_file","file_id":"15325","file_size":398665,"checksum":"839242a9ec1c01158112de25f196e60d","date_updated":"2024-04-16T11:09:37Z","success":1,"date_created":"2024-04-16T11:09:37Z","access_level":"open_access","file_name":"2024_JourStatPhysics_Bossmann.pdf","creator":"dernst"}],"dataavailabilitystatement":"Data sharing not applicable to this article as no datasets were generated or analyzed during the current study."},{"doi":"10.1007/s10240-024-00150-0","quality_controlled":"1","researchdata_availability":"no","abstract":[{"lang":"eng","text":"We study the Fröhlich polaron model in R3, and prove a lower bound on its ground state energy as a function of the total momentum. The bound is asymptotically sharp at large coupling. In combination with a corresponding upper bound proved earlier (Mitrouskas et al. in Forum Math. Sigma 11:1–52, 2023), it shows that the energy is approximately parabolic below the continuum threshold, and that the polaron’s effective mass (defined as the semi-latus rectum of the\r\nparabola) is given by the celebrated Landau–Pekar formula. In particular, it diverges as α4 for large coupling constant α."}],"intvolume":"       140","author":[{"last_name":"Brooks","full_name":"Brooks, Morris","id":"B7ECF9FC-AA38-11E9-AC9A-0930E6697425","first_name":"Morris","orcid":"0000-0002-6249-0928"},{"first_name":"Robert","orcid":"0000-0002-6781-0521","last_name":"Seiringer","id":"4AFD0470-F248-11E8-B48F-1D18A9856A87","full_name":"Seiringer, Robert"}],"publication_status":"published","fulldoi":"https://doi.org/10.1007/s10240-024-00150-0","oa_version":"Published Version","isi":1,"date_published":"2024-12-01T00:00:00Z","publication":"Publications Mathematiques de l'Institut des Hautes Etudes Scientifiques","external_id":{"arxiv":["2211.03353"],"isi":["001297785000001"]},"article_processing_charge":"Yes (in subscription journal)","citation":{"ama":"Brooks M, Seiringer R. The Fröhlich polaron at strong coupling: Part II — Energy-momentum relation and effective mass. <i>Publications Mathematiques de l’Institut des Hautes Etudes Scientifiques</i>. 2024;140:271-309. doi:<a href=\"https://doi.org/10.1007/s10240-024-00150-0\">10.1007/s10240-024-00150-0</a>","ieee":"M. Brooks and R. Seiringer, “The Fröhlich polaron at strong coupling: Part II — Energy-momentum relation and effective mass,” <i>Publications Mathematiques de l’Institut des Hautes Etudes Scientifiques</i>, vol. 140. Springer Nature, pp. 271–309, 2024.","apa":"Brooks, M., &#38; Seiringer, R. (2024). The Fröhlich polaron at strong coupling: Part II — Energy-momentum relation and effective mass. <i>Publications Mathematiques de l’Institut Des Hautes Etudes Scientifiques</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s10240-024-00150-0\">https://doi.org/10.1007/s10240-024-00150-0</a>","ista":"Brooks M, Seiringer R. 2024. The Fröhlich polaron at strong coupling: Part II — Energy-momentum relation and effective mass. Publications Mathematiques de l’Institut des Hautes Etudes Scientifiques. 140, 271–309.","chicago":"Brooks, Morris, and Robert Seiringer. “The Fröhlich Polaron at Strong Coupling: Part II — Energy-Momentum Relation and Effective Mass.” <i>Publications Mathematiques de l’Institut Des Hautes Etudes Scientifiques</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1007/s10240-024-00150-0\">https://doi.org/10.1007/s10240-024-00150-0</a>.","mla":"Brooks, Morris, and Robert Seiringer. “The Fröhlich Polaron at Strong Coupling: Part II — Energy-Momentum Relation and Effective Mass.” <i>Publications Mathematiques de l’Institut Des Hautes Etudes Scientifiques</i>, vol. 140, Springer Nature, 2024, pp. 271–309, doi:<a href=\"https://doi.org/10.1007/s10240-024-00150-0\">10.1007/s10240-024-00150-0</a>.","short":"M. Brooks, R. Seiringer, Publications Mathematiques de l’Institut Des Hautes Etudes Scientifiques 140 (2024) 271–309."},"OA_type":"hybrid","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"17478","year":"2024","publication_identifier":{"issn":["0073-8301"],"eissn":["1618-1913"]},"publisher":"Springer Nature","has_accepted_license":"1","scopus_import":"1","OA_place":"publisher","file_date_updated":"2025-01-13T08:13:42Z","corr_author":"1","file":[{"content_type":"application/pdf","relation":"main_file","file_size":924342,"file_id":"18824","checksum":"af3becc50f7534c9409d3ff8b5c47ed6","success":1,"date_updated":"2025-01-13T08:13:42Z","access_level":"open_access","date_created":"2025-01-13T08:13:42Z","file_name":"2024_PublicMathIHES_Brooks.pdf","creator":"dernst"}],"language":[{"iso":"eng"}],"arxiv":1,"department":[{"_id":"RoSe"}],"volume":140,"article_type":"original","page":"271-309","date_created":"2024-09-01T22:01:08Z","supplementarymaterial":"yes","month":"12","ddc":["510"],"type":"journal_article","day":"01","oa":1,"das_tickbox":"0","title":"The Fröhlich polaron at strong coupling: Part II — Energy-momentum relation and effective mass","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-17T11:07:19Z"},{"oa":1,"editor":[{"first_name":"Angelo","full_name":"Bassi, Angelo","last_name":"Bassi"},{"first_name":"Sheldon","full_name":"Goldstein, Sheldon","last_name":"Goldstein"},{"full_name":"Tumulka, Roderich","last_name":"Tumulka","first_name":"Roderich"},{"full_name":"Zanghi, Nino","last_name":"Zanghi","first_name":"Nino"}],"day":"04","date_updated":"2026-09-17T11:08:01Z","title":"Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications","place":"Cham","das_tickbox":"0","OA_place":"repository","scopus_import":"1","alternative_title":["Fundamental Theories of Physics"],"publisher":"Springer Nature","publication_identifier":{"isbn":["9783031454332"],"eisbn":["9783031454349"],"eissn":["2365-6425"],"issn":["0168-1222"]},"month":"02","supplementarymaterial":"no","type":"book_chapter","date_created":"2025-01-29T10:30:08Z","page":"307-321","volume":215,"acknowledgement":"It is our pleasure to thank Marco Falconi, Nataša Pavlović, Peter Pickl, Robert Seiringer and Avy Soffer for the collaboration on the works [11, 13, 14, 21, 33, 39]. L.B. was supported by the German Research Foundation within the Munich Center of Quantum Science and Technology (EXC 2111). N.L. acknowledges support from the Swiss National Science Foundation through the NCCR SwissMap and funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 101024712. S.P. acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - project number 512258249.","main_file_link":[{"open_access":"1","url":"https://doi.org/10.48550/arXiv.2304.12910"}],"department":[{"_id":"RoSe"}],"arxiv":1,"language":[{"iso":"eng"}],"series_title":"FTPH","citation":{"ieee":"L. Bossmann, N. Leopold, D. J. Mitrouskas, and S. Petrat, “Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications,” in <i>Physics and the Nature of Reality</i>, vol. 215, A. Bassi, S. Goldstein, R. Tumulka, and N. Zanghi, Eds. Cham: Springer Nature, 2024, pp. 307–321.","ista":"Bossmann L, Leopold N, Mitrouskas DJ, Petrat S. 2024.Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications. In: Physics and the Nature of Reality. Fundamental Theories of Physics, vol. 215, 307–321.","apa":"Bossmann, L., Leopold, N., Mitrouskas, D. J., &#38; Petrat, S. (2024). Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications. In A. Bassi, S. Goldstein, R. Tumulka, &#38; N. Zanghi (Eds.), <i>Physics and the Nature of Reality</i> (Vol. 215, pp. 307–321). Cham: Springer Nature. <a href=\"https://doi.org/10.1007/978-3-031-45434-9_22\">https://doi.org/10.1007/978-3-031-45434-9_22</a>","chicago":"Bossmann, Lea, Nikolai Leopold, David Johannes Mitrouskas, and Sören Petrat. “Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications.” In <i>Physics and the Nature of Reality</i>, edited by Angelo Bassi, Sheldon Goldstein, Roderich Tumulka, and Nino Zanghi, 215:307–21. FTPH. Cham: Springer Nature, 2024. <a href=\"https://doi.org/10.1007/978-3-031-45434-9_22\">https://doi.org/10.1007/978-3-031-45434-9_22</a>.","mla":"Bossmann, Lea, et al. “Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications.” <i>Physics and the Nature of Reality</i>, edited by Angelo Bassi et al., vol. 215, Springer Nature, 2024, pp. 307–21, doi:<a href=\"https://doi.org/10.1007/978-3-031-45434-9_22\">10.1007/978-3-031-45434-9_22</a>.","short":"L. Bossmann, N. Leopold, D.J. Mitrouskas, S. Petrat, in:, A. Bassi, S. Goldstein, R. Tumulka, N. Zanghi (Eds.), Physics and the Nature of Reality, Springer Nature, Cham, 2024, pp. 307–321.","ama":"Bossmann L, Leopold N, Mitrouskas DJ, Petrat S. Asymptotic Analysis of the Weakly Interacting Bose Gas: A Collection of Recent Results and Applications. In: Bassi A, Goldstein S, Tumulka R, Zanghi N, eds. <i>Physics and the Nature of Reality</i>. Vol 215. FTPH. Cham: Springer Nature; 2024:307-321. doi:<a href=\"https://doi.org/10.1007/978-3-031-45434-9_22\">10.1007/978-3-031-45434-9_22</a>"},"article_processing_charge":"No","external_id":{"arxiv":["2304.12910"]},"publication":"Physics and the Nature of Reality","year":"2024","_id":"18948","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","OA_type":"green","researchdata_availability":"no","quality_controlled":"1","doi":"10.1007/978-3-031-45434-9_22","oa_version":"Preprint","fulldoi":"https://doi.org/10.1007/978-3-031-45434-9_22","date_published":"2024-02-04T00:00:00Z","publication_status":"published","author":[{"orcid":"0000-0002-6854-1343","first_name":"Lea","full_name":"Bossmann, Lea","id":"A2E3BCBE-5FCC-11E9-AA4B-76F3E5697425","last_name":"Bossmann"},{"last_name":"Leopold","full_name":"Leopold, Nikolai","first_name":"Nikolai"},{"first_name":"David Johannes","last_name":"Mitrouskas","full_name":"Mitrouskas, David Johannes","id":"cbddacee-2b11-11eb-a02e-a2e14d04e52d"},{"full_name":"Petrat, Sören","last_name":"Petrat","first_name":"Sören"}],"intvolume":"       215","abstract":[{"lang":"eng","text":"We consider a gas of N bosons with interactions in the mean-field scaling regime. We review a recent proof of the asymptotic expansion of its spectrum and eigenstates and two applications of this result, namely the derivation of an Edgeworth expansion for fluctuations of one-body operators and the computation of the binding energy of an inhomogeneous Bose gas to any order. Finally, we collect related results for the dynamics of the weakly interacting Bose gas and for the regularized Nelson model."}]},{"fulldoi":"https://doi.org/10.1212/NXI.0000000000200284","date_published":"2024-09-01T00:00:00Z","isi":1,"oa_version":"Published Version","project":[{"name":"LGI1 antibody-induced pathophysiology in synapses","grant_number":"I04638","_id":"05970B30-7A3F-11EA-A408-12923DDC885E"}],"intvolume":"        11","abstract":[{"text":"Autoantibodies against the protein leucine-rich glioma inactivated 1 (LGI1) cause the most\r\ncommon subtype of autoimmune encephalitis with predominant involvement of the limbic\r\nsystem, associated with seizures and memory deficits. LGI1 and its receptor ADAM22 are part\r\nof a transsynaptic protein complex that includes several proteins involved in presynaptic\r\nneurotransmitter release and postsynaptic glutamate sensing. Autoantibodies against LGI1\r\nincrease excitatory synaptic strength, but studies that genetically disrupt the LGI1-ADAM22\r\ncomplex report a reduction in postsynaptic glutamate receptor-mediated responses. Thus, the\r\nmechanisms underlying the increased synaptic strength induced by LGI1 autoantibodies remain elusive, and the contributions of presynaptic molecules to the LGI1-transsynaptic complex remain unclear. We therefore investigated the presynaptic mechanisms that mediate\r\nautoantibody-induced synaptic strengthening.","lang":"eng"}],"issue":"5","author":[{"full_name":"Ritzau-Jost, Andreas","last_name":"Ritzau-Jost","first_name":"Andreas"},{"first_name":"Felix","full_name":"Gsell, Felix","last_name":"Gsell"},{"first_name":"Josefine","last_name":"Sell","full_name":"Sell, Josefine"},{"first_name":"Stefan","full_name":"Sachs, Stefan","last_name":"Sachs"},{"id":"3786AB44-F248-11E8-B48F-1D18A9856A87","full_name":"Montanaro-Punzengruber, Jacqueline-Claire","last_name":"Montanaro-Punzengruber","first_name":"Jacqueline-Claire"},{"first_name":"Toni","full_name":"Kirmann, Toni","last_name":"Kirmann"},{"full_name":"Maaß, Sebastian","last_name":"Maaß","first_name":"Sebastian"},{"first_name":"Sarosh R.","full_name":"Irani, Sarosh R.","last_name":"Irani"},{"first_name":"Christian","full_name":"Werner, Christian","last_name":"Werner"},{"full_name":"Geis, Christian","last_name":"Geis","first_name":"Christian"},{"first_name":"Markus","last_name":"Sauer","full_name":"Sauer, Markus"},{"full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","orcid":"0000-0001-8761-9444","first_name":"Ryuichi"},{"first_name":"Stefan","full_name":"Hallermann, Stefan","last_name":"Hallermann"}],"publication_status":"published","researchdata_availability":"upon request","doi":"10.1212/NXI.0000000000200284","quality_controlled":"1","year":"2024","OA_type":"gold","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"17457","external_id":{"pmid":["39141878"],"isi":["001291908600001"]},"article_processing_charge":"Yes","citation":{"ama":"Ritzau-Jost A, Gsell F, Sell J, et al. LGI1 autoantibodies enhance synaptic transmission by presynaptic Kv1 loss and increased action potential broadening. <i>Neurology, Neuroimmunology &#38; Neuroinflammation</i>. 2024;11(5):e200284. doi:<a href=\"https://doi.org/10.1212/NXI.0000000000200284\">10.1212/NXI.0000000000200284</a>","chicago":"Ritzau-Jost, Andreas, Felix Gsell, Josefine Sell, Stefan Sachs, Jacqueline-Claire Montanaro-Punzengruber, Toni Kirmann, Sebastian Maaß, et al. “LGI1 Autoantibodies Enhance Synaptic Transmission by Presynaptic Kv1 Loss and Increased Action Potential Broadening.” <i>Neurology, Neuroimmunology &#38; Neuroinflammation</i>. Wolters Kluwer, 2024. <a href=\"https://doi.org/10.1212/NXI.0000000000200284\">https://doi.org/10.1212/NXI.0000000000200284</a>.","mla":"Ritzau-Jost, Andreas, et al. “LGI1 Autoantibodies Enhance Synaptic Transmission by Presynaptic Kv1 Loss and Increased Action Potential Broadening.” <i>Neurology, Neuroimmunology &#38; Neuroinflammation</i>, vol. 11, no. 5, Wolters Kluwer, 2024, p. e200284, doi:<a href=\"https://doi.org/10.1212/NXI.0000000000200284\">10.1212/NXI.0000000000200284</a>.","short":"A. Ritzau-Jost, F. Gsell, J. Sell, S. Sachs, J.-C. Montanaro-Punzengruber, T. Kirmann, S. Maaß, S.R. Irani, C. Werner, C. Geis, M. Sauer, R. Shigemoto, S. Hallermann, Neurology, Neuroimmunology &#38; Neuroinflammation 11 (2024) e200284.","ieee":"A. Ritzau-Jost <i>et al.</i>, “LGI1 autoantibodies enhance synaptic transmission by presynaptic Kv1 loss and increased action potential broadening,” <i>Neurology, Neuroimmunology &#38; Neuroinflammation</i>, vol. 11, no. 5. Wolters Kluwer, p. e200284, 2024.","ista":"Ritzau-Jost A, Gsell F, Sell J, Sachs S, Montanaro-Punzengruber J-C, Kirmann T, Maaß S, Irani SR, Werner C, Geis C, Sauer M, Shigemoto R, Hallermann S. 2024. LGI1 autoantibodies enhance synaptic transmission by presynaptic Kv1 loss and increased action potential broadening. Neurology, Neuroimmunology &#38; Neuroinflammation. 11(5), e200284.","apa":"Ritzau-Jost, A., Gsell, F., Sell, J., Sachs, S., Montanaro-Punzengruber, J.-C., Kirmann, T., … Hallermann, S. (2024). LGI1 autoantibodies enhance synaptic transmission by presynaptic Kv1 loss and increased action potential broadening. <i>Neurology, Neuroimmunology &#38; Neuroinflammation</i>. Wolters Kluwer. <a href=\"https://doi.org/10.1212/NXI.0000000000200284\">https://doi.org/10.1212/NXI.0000000000200284</a>"},"publication":"Neurology, Neuroimmunology & Neuroinflammation","acknowledgement":"The authors thank Claudia Sommer for expert technical assistance, the Electron Microscopy Facility of IST-Austria for resources, and Tereza Belinova in the Imaging and Optics Facility of IST-Austria for 3D reconstruction. ","volume":11,"article_type":"original","pmid":1,"page":"e200284","date_created":"2024-08-25T22:01:07Z","type":"journal_article","ddc":["570"],"supplementarymaterial":"yes","month":"09","file":[{"checksum":"1e6d1230e0387f72752e3268f5330c9e","file_size":855818,"file_id":"18815","relation":"main_file","content_type":"application/pdf","creator":"dernst","file_name":"2024_NeurologyNeuroimmNeuroinflamm_RitzauJost.pdf","date_created":"2025-01-09T13:42:42Z","access_level":"open_access","date_updated":"2025-01-09T13:42:42Z","success":1}],"dataavailabilitystatement":"Data that support the findings of this study are available from the corresponding author on reasonable request.","language":[{"iso":"eng"}],"department":[{"_id":"RySh"}],"has_accepted_license":"1","scopus_import":"1","OA_place":"publisher","file_date_updated":"2025-01-09T13:42:42Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"}],"publication_identifier":{"eissn":["2332-7812"]},"publisher":"Wolters Kluwer","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-17T11:27:41Z","das_tickbox":"1","DOAJ_listed":"1","title":"LGI1 autoantibodies enhance synaptic transmission by presynaptic Kv1 loss and increased action potential broadening","oa":1,"related_material":{"link":[{"relation":"earlier_version","url":"https://doi.org/10.1101/2023.10.04.560631"}]},"day":"01"},{"publication_identifier":{"issn":["1660-8933"],"eissn":["1660-8941"]},"publisher":"EMS Press","volume":20,"article_type":"original","acknowledgement":"The MFO and the workshop organizers would like to thank the National Science Foundation for supporting the participation of junior researchers in the workshop by the grant DMS-2230648, “US Junior Oberwolfach Fellows”.","date_created":"2025-01-29T13:09:28Z","page":"2247-2302","type":"journal_article","month":"04","supplementarymaterial":"no","language":[{"iso":"eng"}],"department":[{"_id":"RoSe"}],"day":"18","date_updated":"2026-09-17T11:09:16Z","das_tickbox":"0","title":"Many-body quantum systems","researchdata_availability":"no","doi":"10.4171/owr/2023/39","quality_controlled":"1","oa_version":"None","fulldoi":"https://doi.org/10.4171/owr/2023/39","date_published":"2024-04-18T00:00:00Z","intvolume":"        20","abstract":[{"text":"This workshop brought together experts on the analysis of quantum many-body problems and quantum statistical mechanics, with the goal of discussing the state-of-the-art of the field, recent developments as well as challenges for the future. The main topics of discussion concerned the equilibrium and dynamical behavior of (bosonic or fermionic) quantum gases, quantum spin systems, as well as quantum field theory models like the Nelson or Fröhlich model.","lang":"eng"}],"author":[{"first_name":"Christian","full_name":"Hainzl, Christian","last_name":"Hainzl"},{"last_name":"Schlein","full_name":"Schlein, Benjamin","first_name":"Benjamin"},{"first_name":"Robert","orcid":"0000-0002-6781-0521","last_name":"Seiringer","id":"4AFD0470-F248-11E8-B48F-1D18A9856A87","full_name":"Seiringer, Robert"},{"first_name":"Simone","last_name":"Warzel","full_name":"Warzel, Simone"}],"publication_status":"published","issue":"3","article_processing_charge":"No","citation":{"apa":"Hainzl, C., Schlein, B., Seiringer, R., &#38; Warzel, S. (2024). Many-body quantum systems. <i>Oberwolfach Reports</i>. EMS Press. <a href=\"https://doi.org/10.4171/owr/2023/39\">https://doi.org/10.4171/owr/2023/39</a>","ieee":"C. Hainzl, B. Schlein, R. Seiringer, and S. Warzel, “Many-body quantum systems,” <i>Oberwolfach Reports</i>, vol. 20, no. 3. EMS Press, pp. 2247–2302, 2024.","ista":"Hainzl C, Schlein B, Seiringer R, Warzel S. 2024. Many-body quantum systems. Oberwolfach Reports. 20(3), 2247–2302.","chicago":"Hainzl, Christian, Benjamin Schlein, Robert Seiringer, and Simone Warzel. “Many-Body Quantum Systems.” <i>Oberwolfach Reports</i>. EMS Press, 2024. <a href=\"https://doi.org/10.4171/owr/2023/39\">https://doi.org/10.4171/owr/2023/39</a>.","mla":"Hainzl, Christian, et al. “Many-Body Quantum Systems.” <i>Oberwolfach Reports</i>, vol. 20, no. 3, EMS Press, 2024, pp. 2247–302, doi:<a href=\"https://doi.org/10.4171/owr/2023/39\">10.4171/owr/2023/39</a>.","short":"C. Hainzl, B. Schlein, R. Seiringer, S. Warzel, Oberwolfach Reports 20 (2024) 2247–2302.","ama":"Hainzl C, Schlein B, Seiringer R, Warzel S. Many-body quantum systems. <i>Oberwolfach Reports</i>. 2024;20(3):2247-2302. doi:<a href=\"https://doi.org/10.4171/owr/2023/39\">10.4171/owr/2023/39</a>"},"publication":"Oberwolfach Reports","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"18958"},{"fulldoi":"https://doi.org/10.1016/j.bpj.2023.12.020","date_published":"2024-02-06T00:00:00Z","project":[{"name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","grant_number":"802960","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","call_identifier":"H2020"}],"isi":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Many cell functions require a concerted effort from multiple membrane proteins, for example, for signaling, cell division, and endocytosis. One contribution to their successful self-organization stems from the membrane deformations that these proteins induce. While the pairwise interaction potential of two membrane-deforming spheres has recently been measured, membrane-deformation-induced interactions have been predicted to be nonadditive, and hence their collective behavior cannot be deduced from this measurement. We here employ a colloidal model system consisting of adhesive spheres and giant unilamellar vesicles to test these predictions by measuring the interaction potential of the simplest case of three membrane-deforming, spherical particles. We quantify their interactions and arrangements and, for the first time, experimentally confirm and quantify the nonadditive nature of membrane-deformation-induced interactions. We furthermore conclude that there exist two favorable configurations on the membrane: (1) a linear and (2) a triangular arrangement of the three spheres. Using Monte Carlo simulations, we corroborate the experimentally observed energy minima and identify a lowering of the membrane deformation as the cause for the observed configurations. The high symmetry of the preferred arrangements for three particles suggests that arrangements of many membrane-deforming objects might follow simple rules."}],"intvolume":"       123","issue":"3","author":[{"last_name":"Azadbakht","full_name":"Azadbakht, Ali","first_name":"Ali"},{"last_name":"Meadowcroft","id":"a4725fd6-932b-11ed-81e2-c098c7f37ae1","full_name":"Meadowcroft, Billie","first_name":"Billie","orcid":"0000-0003-3441-1337"},{"first_name":"Juraj","full_name":"Majek, Juraj","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","last_name":"Majek"},{"full_name":"Šarić, Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","last_name":"Šarić","orcid":"0000-0002-7854-2139","first_name":"Anđela"},{"first_name":"Daniela J.","last_name":"Kraft","full_name":"Kraft, Daniela J."}],"publication_status":"published","researchdata_availability":"no","quality_controlled":"1","doi":"10.1016/j.bpj.2023.12.020","year":"2024","_id":"14844","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_processing_charge":"Yes (in subscription journal)","external_id":{"isi":["001185235900001"],"pmid":["38158654"]},"citation":{"apa":"Azadbakht, A., Meadowcroft, B., Majek, J., Šarić, A., &#38; Kraft, D. J. (2024). Nonadditivity in interactions between three membrane-wrapped colloidal spheres. <i>Biophysical Journal</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bpj.2023.12.020\">https://doi.org/10.1016/j.bpj.2023.12.020</a>","ista":"Azadbakht A, Meadowcroft B, Majek J, Šarić A, Kraft DJ. 2024. Nonadditivity in interactions between three membrane-wrapped colloidal spheres. Biophysical Journal. 123(3), 307–316.","ieee":"A. Azadbakht, B. Meadowcroft, J. Majek, A. Šarić, and D. J. Kraft, “Nonadditivity in interactions between three membrane-wrapped colloidal spheres,” <i>Biophysical Journal</i>, vol. 123, no. 3. Elsevier, pp. 307–316, 2024.","short":"A. Azadbakht, B. Meadowcroft, J. Majek, A. Šarić, D.J. Kraft, Biophysical Journal 123 (2024) 307–316.","mla":"Azadbakht, Ali, et al. “Nonadditivity in Interactions between Three Membrane-Wrapped Colloidal Spheres.” <i>Biophysical Journal</i>, vol. 123, no. 3, Elsevier, 2024, pp. 307–16, doi:<a href=\"https://doi.org/10.1016/j.bpj.2023.12.020\">10.1016/j.bpj.2023.12.020</a>.","chicago":"Azadbakht, Ali, Billie Meadowcroft, Juraj Majek, Anđela Šarić, and Daniela J. Kraft. “Nonadditivity in Interactions between Three Membrane-Wrapped Colloidal Spheres.” <i>Biophysical Journal</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.bpj.2023.12.020\">https://doi.org/10.1016/j.bpj.2023.12.020</a>.","ama":"Azadbakht A, Meadowcroft B, Majek J, Šarić A, Kraft DJ. Nonadditivity in interactions between three membrane-wrapped colloidal spheres. <i>Biophysical Journal</i>. 2024;123(3):307-316. doi:<a href=\"https://doi.org/10.1016/j.bpj.2023.12.020\">10.1016/j.bpj.2023.12.020</a>"},"publication":"Biophysical Journal","pmid":1,"page":"307-316","date_created":"2024-01-21T23:00:56Z","article_type":"original","acknowledgement":"We gratefully acknowledge useful discussions with Casper van der Wel, help by Yogesh Shelke with PAA coverslip preparation, and support by Rachel Doherty with particle functionalization. A.A. and D.J.K. would like to thank Timon Idema and George Dadunashvili for initial attempts to simulate the experimental system. D.J.K. would like to thank the physics department at Leiden University for funding the PhD position of A.A. B.M. and A.Š. acknowledge funding by the European Union’s Horizon 2020 research and innovation programme (ERC starting grant no. 802960).","volume":123,"type":"journal_article","supplementarymaterial":"no","ddc":["570"],"month":"02","file":[{"file_name":"2024_BiophysicalJournal_Azadbakht.pdf","creator":"dernst","success":1,"date_updated":"2024-07-16T12:09:55Z","date_created":"2024-07-16T12:09:55Z","access_level":"open_access","file_size":3189926,"file_id":"17266","checksum":"1c8fe1cf950394875b65b90da86428ff","content_type":"application/pdf","relation":"main_file"}],"department":[{"_id":"AnSa"}],"language":[{"iso":"eng"}],"has_accepted_license":"1","scopus_import":"1","file_date_updated":"2024-07-16T12:09:55Z","ec_funded":1,"publication_identifier":{"eissn":["1542-0086"],"issn":["0006-3495"]},"publisher":"Elsevier","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"date_updated":"2026-09-24T10:26:59Z","das_tickbox":"0","title":"Nonadditivity in interactions between three membrane-wrapped colloidal spheres","oa":1,"day":"06"},{"oa":1,"day":"13","related_material":{"record":[{"status":"public","id":"15027","relation":"research_data"}]},"date_updated":"2026-09-24T10:27:36Z","APC_amount":"5936,71 EUR","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"title":"Self-replication of Aβ42 aggregates occurs on small and isolated fibril sites","das_tickbox":"1","file_date_updated":"2024-02-26T08:20:00Z","OA_place":"publisher","scopus_import":"1","corr_author":"1","has_accepted_license":"1","publisher":"National Academy of Sciences","publication_identifier":{"eissn":["1091-6490"]},"ec_funded":1,"ddc":["570"],"type":"journal_article","month":"02","supplementarymaterial":"no","volume":121,"acknowledgement":"We acknowledge support from the Erasmus programme and the University College London Institute for the Physics of Living Systems (S.C., T.C.T.M., A.Š.), the Biotechnology and Biological Sciences Research Council (T.P.J.K.), the Engineering and Physical Sciences Research Council (D.F.), the European Research Council (T.P.J.K., S.L., D.F., and A.Š.), the Frances and Augustus Newman Foundation (T.P.J.K.), the Academy of Medical Sciences and Wellcome Trust (A.Š.), and the Royal Society (S.C. and A.Š.).","article_type":"original","date_created":"2024-02-18T23:01:00Z","pmid":1,"language":[{"iso":"eng"}],"department":[{"_id":"AnSa"}],"dataavailabilitystatement":"Aggregation data and the source code for the coarse-grained computer model have been deposited in Figshare (https://figshare.com/s/85798bba4ebc68d822ed)","file":[{"relation":"main_file","content_type":"application/pdf","checksum":"5aeb65bcc0dd829b1f9ab307c5031d4b","file_size":7699487,"file_id":"15026","date_created":"2024-02-26T08:20:00Z","access_level":"open_access","success":1,"date_updated":"2024-02-26T08:20:00Z","creator":"dernst","file_name":"2024_PNAS_Curk.pdf"}],"citation":{"apa":"Curk, S., Krausser, J., Meisl, G., Frenkel, D., Linse, S., Michaels, T. C. T., … Šarić, A. (2024). Self-replication of Aβ42 aggregates occurs on small and isolated fibril sites. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2220075121\">https://doi.org/10.1073/pnas.2220075121</a>","ieee":"S. Curk <i>et al.</i>, “Self-replication of Aβ42 aggregates occurs on small and isolated fibril sites,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 7. National Academy of Sciences, 2024.","ista":"Curk S, Krausser J, Meisl G, Frenkel D, Linse S, Michaels TCT, Knowles TPJ, Šarić A. 2024. Self-replication of Aβ42 aggregates occurs on small and isolated fibril sites. Proceedings of the National Academy of Sciences of the United States of America. 121(7), e2220075121.","chicago":"Curk, Samo, Johannes Krausser, Georg Meisl, Daan Frenkel, Sara Linse, Thomas C.T. Michaels, Tuomas P.J. Knowles, and Anđela Šarić. “Self-Replication of Aβ42 Aggregates Occurs on Small and Isolated Fibril Sites.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2024. <a href=\"https://doi.org/10.1073/pnas.2220075121\">https://doi.org/10.1073/pnas.2220075121</a>.","mla":"Curk, Samo, et al. “Self-Replication of Aβ42 Aggregates Occurs on Small and Isolated Fibril Sites.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 7, e2220075121, National Academy of Sciences, 2024, doi:<a href=\"https://doi.org/10.1073/pnas.2220075121\">10.1073/pnas.2220075121</a>.","short":"S. Curk, J. Krausser, G. Meisl, D. Frenkel, S. Linse, T.C.T. Michaels, T.P.J. Knowles, A. Šarić, Proceedings of the National Academy of Sciences of the United States of America 121 (2024).","ama":"Curk S, Krausser J, Meisl G, et al. Self-replication of Aβ42 aggregates occurs on small and isolated fibril sites. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2024;121(7). doi:<a href=\"https://doi.org/10.1073/pnas.2220075121\">10.1073/pnas.2220075121</a>"},"external_id":{"isi":["001169063600007"],"pmid":["38335256"]},"article_processing_charge":"Yes (in subscription journal)","publication":"Proceedings of the National Academy of Sciences of the United States of America","article_number":"e2220075121","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"15001","OA_type":"hybrid","researchdata_availability":"yes","doi":"10.1073/pnas.2220075121","quality_controlled":"1","fulldoi":"https://doi.org/10.1073/pnas.2220075121","oa_version":"Published Version","date_published":"2024-02-13T00:00:00Z","project":[{"call_identifier":"H2020","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","grant_number":"802960"}],"isi":1,"author":[{"first_name":"Samo","orcid":"0000-0001-6160-9766","last_name":"Curk","full_name":"Curk, Samo","id":"031eff0d-d481-11ee-8508-cd12a7a86e5b"},{"last_name":"Krausser","full_name":"Krausser, Johannes","first_name":"Johannes"},{"full_name":"Meisl, Georg","last_name":"Meisl","first_name":"Georg"},{"last_name":"Frenkel","full_name":"Frenkel, Daan","first_name":"Daan"},{"full_name":"Linse, Sara","last_name":"Linse","first_name":"Sara"},{"last_name":"Michaels","full_name":"Michaels, Thomas C.T.","first_name":"Thomas C.T."},{"full_name":"Knowles, Tuomas P.J.","last_name":"Knowles","first_name":"Tuomas P.J."},{"last_name":"Šarić","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","full_name":"Šarić, Anđela","first_name":"Anđela","orcid":"0000-0002-7854-2139"}],"publication_status":"published","issue":"7","abstract":[{"lang":"eng","text":"Self-replication of amyloid fibrils via secondary nucleation is an intriguing physicochemical phenomenon in which existing fibrils catalyze the formation of their own copies. The molecular events behind this fibril surface-mediated process remain largely inaccessible to current structural and imaging techniques. Using statistical mechanics, computer modeling, and chemical kinetics, we show that the catalytic structure of the fibril surface can be inferred from the aggregation behavior in the presence and absence of a fibril-binding inhibitor. We apply our approach to the case of Alzheimer’s A\r\n amyloid fibrils formed in the presence of proSP-C Brichos inhibitors. We find that self-replication of A\r\n fibrils occurs on small catalytic sites on the fibril surface, which are far apart from each other, and each of which can be covered by a single Brichos inhibitor."}],"intvolume":"       121"},{"has_accepted_license":"1","scopus_import":"1","file_date_updated":"2024-05-27T06:37:01Z","publication_identifier":{"eissn":["2643-1564"]},"publisher":"American Physical Society","article_type":"original","volume":6,"acknowledgement":"D.M. acknowledges the support of the Royal Society via a University Research Fellowship. This project has received support from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Program (Grant Agreement No. 947918 to D.M. and No. 677532 to M.L.). The authors acknowledge insightful discussions with Daan Noordermeer and Antonio Valdes, who also kindly gifted us with the 1288 plasmid.","date_created":"2024-05-26T22:00:58Z","ddc":["530"],"month":"05","supplementarymaterial":"no","type":"journal_article","file":[{"success":1,"date_updated":"2024-05-27T06:37:01Z","access_level":"open_access","date_created":"2024-05-27T06:37:01Z","file_name":"2024_PhysicalReviewResearch_Panoukidou.pdf","creator":"dernst","content_type":"application/pdf","relation":"main_file","file_id":"17055","file_size":1409416,"checksum":"63a962d49ef1e21a3367d265784df14b"}],"language":[{"iso":"eng"}],"arxiv":1,"department":[{"_id":"AnSa"}],"oa":1,"day":"01","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-24T10:26:36Z","das_tickbox":"0","DOAJ_listed":"1","title":"Runaway transition in irreversible polymer condensation with cyclization","researchdata_availability":"no","doi":"10.1103/PhysRevResearch.6.023189","quality_controlled":"1","fulldoi":"https://doi.org/10.1103/PhysRevResearch.6.023189","oa_version":"Published Version","date_published":"2024-05-01T00:00:00Z","abstract":[{"lang":"eng","text":"The process of polymer condensation, i.e., the formation of bonds between reactive end groups, is ubiquitous in both industry and biology. Here we study generic systems undergoing polymer condensation in competition with cyclization. Using a generalized Smoluchowski theory, molecular dynamics simulations and experiments with DNA and ATP-consuming T4 ligase, we find that this system displays a transition, from a ring-dominated regime with finite-length chains at infinite time to a linear-polymers-dominated one with chains that keep growing in time. Finally, we show that fluids prepared close to the transition may have widely different compositions and rheology at large condensation times."}],"intvolume":"         6","issue":"2","publication_status":"published","author":[{"last_name":"Panoukidou","full_name":"Panoukidou, Maria","first_name":"Maria"},{"last_name":"Weir","full_name":"Weir, Simon","first_name":"Simon"},{"orcid":"0000-0002-9645-6576","first_name":"Valerio","full_name":"Sorichetti, Valerio","id":"ef8a92cb-c7b6-11ec-8bea-e1fd5847bc5b","last_name":"Sorichetti"},{"last_name":"Fosado","full_name":"Fosado, Yair Gutierrez","first_name":"Yair Gutierrez"},{"full_name":"Lenz, Martin","last_name":"Lenz","first_name":"Martin"},{"first_name":"Davide","full_name":"Michieletto, Davide","last_name":"Michieletto"}],"external_id":{"arxiv":["2210.14010"]},"article_processing_charge":"Yes","citation":{"ista":"Panoukidou M, Weir S, Sorichetti V, Fosado YG, Lenz M, Michieletto D. 2024. Runaway transition in irreversible polymer condensation with cyclization. Physical Review Research. 6(2), 023189.","apa":"Panoukidou, M., Weir, S., Sorichetti, V., Fosado, Y. G., Lenz, M., &#38; Michieletto, D. (2024). Runaway transition in irreversible polymer condensation with cyclization. <i>Physical Review Research</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevResearch.6.023189\">https://doi.org/10.1103/PhysRevResearch.6.023189</a>","ieee":"M. Panoukidou, S. Weir, V. Sorichetti, Y. G. Fosado, M. Lenz, and D. Michieletto, “Runaway transition in irreversible polymer condensation with cyclization,” <i>Physical Review Research</i>, vol. 6, no. 2. American Physical Society, 2024.","chicago":"Panoukidou, Maria, Simon Weir, Valerio Sorichetti, Yair Gutierrez Fosado, Martin Lenz, and Davide Michieletto. “Runaway Transition in Irreversible Polymer Condensation with Cyclization.” <i>Physical Review Research</i>. American Physical Society, 2024. <a href=\"https://doi.org/10.1103/PhysRevResearch.6.023189\">https://doi.org/10.1103/PhysRevResearch.6.023189</a>.","mla":"Panoukidou, Maria, et al. “Runaway Transition in Irreversible Polymer Condensation with Cyclization.” <i>Physical Review Research</i>, vol. 6, no. 2, 023189, American Physical Society, 2024, doi:<a href=\"https://doi.org/10.1103/PhysRevResearch.6.023189\">10.1103/PhysRevResearch.6.023189</a>.","short":"M. Panoukidou, S. Weir, V. Sorichetti, Y.G. Fosado, M. Lenz, D. Michieletto, Physical Review Research 6 (2024).","ama":"Panoukidou M, Weir S, Sorichetti V, Fosado YG, Lenz M, Michieletto D. Runaway transition in irreversible polymer condensation with cyclization. <i>Physical Review Research</i>. 2024;6(2). doi:<a href=\"https://doi.org/10.1103/PhysRevResearch.6.023189\">10.1103/PhysRevResearch.6.023189</a>"},"article_number":"023189","publication":"Physical Review Research","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"17050"},{"year":"2024","status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"15116","external_id":{"pmid":["38451946"],"isi":["001206387400001"]},"article_processing_charge":"Yes (in subscription journal)","citation":{"apa":"Giubertoni, G., Feng, L., Klein, K., Giannetti, G., Rutten, L., Choi, Y., … Woutersen, S. (2024). Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2313162121\">https://doi.org/10.1073/pnas.2313162121</a>","ista":"Giubertoni G, Feng L, Klein K, Giannetti G, Rutten L, Choi Y, Van Der Net A, Castro-Linares G, Caporaletti F, Micha D, Hunger J, Deblais A, Bonn D, Sommerdijk N, Šarić A, Ilie IM, Koenderink GH, Woutersen S. 2024. Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration. Proceedings of the National Academy of Sciences of the United States of America. 121(11), e2313162121.","ieee":"G. Giubertoni <i>et al.</i>, “Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 11. National Academy of Sciences, 2024.","chicago":"Giubertoni, Giulia, Liru Feng, Kevin Klein, Guido Giannetti, Luco Rutten, Yeji Choi, Anouk Van Der Net, et al. “Elucidating the Role of Water in Collagen Self-Assembly by Isotopically Modulating Collagen Hydration.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2024. <a href=\"https://doi.org/10.1073/pnas.2313162121\">https://doi.org/10.1073/pnas.2313162121</a>.","mla":"Giubertoni, Giulia, et al. “Elucidating the Role of Water in Collagen Self-Assembly by Isotopically Modulating Collagen Hydration.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 11, e2313162121, National Academy of Sciences, 2024, doi:<a href=\"https://doi.org/10.1073/pnas.2313162121\">10.1073/pnas.2313162121</a>.","short":"G. Giubertoni, L. Feng, K. Klein, G. Giannetti, L. Rutten, Y. Choi, A. Van Der Net, G. Castro-Linares, F. Caporaletti, D. Micha, J. Hunger, A. Deblais, D. Bonn, N. Sommerdijk, A. Šarić, I.M. Ilie, G.H. Koenderink, S. Woutersen, Proceedings of the National Academy of Sciences of the United States of America 121 (2024).","ama":"Giubertoni G, Feng L, Klein K, et al. Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2024;121(11). doi:<a href=\"https://doi.org/10.1073/pnas.2313162121\">10.1073/pnas.2313162121</a>"},"article_number":"e2313162121","publication":"Proceedings of the National Academy of Sciences of the United States of America","fulldoi":"https://doi.org/10.1073/pnas.2313162121","isi":1,"oa_version":"Published Version","date_published":"2024-03-12T00:00:00Z","intvolume":"       121","abstract":[{"lang":"eng","text":"Water is known to play an important role in collagen self-assembly, but it is still largely unclear how water–collagen interactions influence the assembly process and determine the fibril network properties. Here, we use the H2O/D2O isotope effect on the hydrogen-bond strength in water to investigate the role of hydration in collagen self-assembly. We dissolve collagen in H2O and D2O and compare the growth kinetics and the structure of the collagen assemblies formed in these water isotopomers. Surprisingly, collagen assembly occurs ten times faster in D2O than in H2O, and collagen in D2O self-assembles into much thinner fibrils, that form a more inhomogeneous and softer network, with a fourfold reduction in elastic modulus when compared to H2O. Combining spectroscopic measurements with atomistic simulations, we show that collagen in D2O is less hydrated than in H2O. This partial dehydration lowers the enthalpic penalty for water removal and reorganization at the collagen–water interface, increasing the self-assembly rate and the number of nucleation centers, leading to thinner fibrils and a softer network. Coarse-grained simulations show that the acceleration in the initial nucleation rate can be reproduced by the enhancement of electrostatic interactions. These results show that water acts as a mediator between collagen monomers, by modulating their interactions so as to optimize the assembly process and, thus, the final network properties. We believe that isotopically modulating the hydration of proteins can be a valuable method to investigate the role of water in protein structural dynamics and protein self-assembly."}],"publication_status":"published","issue":"11","author":[{"first_name":"Giulia","full_name":"Giubertoni, Giulia","last_name":"Giubertoni"},{"first_name":"Liru","full_name":"Feng, Liru","last_name":"Feng"},{"full_name":"Klein, Kevin","last_name":"Klein","first_name":"Kevin"},{"first_name":"Guido","full_name":"Giannetti, Guido","last_name":"Giannetti"},{"first_name":"Luco","last_name":"Rutten","full_name":"Rutten, Luco"},{"first_name":"Yeji","full_name":"Choi, Yeji","last_name":"Choi"},{"first_name":"Anouk","full_name":"Van Der Net, Anouk","last_name":"Van Der Net"},{"first_name":"Gerard","last_name":"Castro-Linares","full_name":"Castro-Linares, Gerard"},{"first_name":"Federico","full_name":"Caporaletti, Federico","last_name":"Caporaletti"},{"first_name":"Dimitra","full_name":"Micha, Dimitra","last_name":"Micha"},{"last_name":"Hunger","full_name":"Hunger, Johannes","first_name":"Johannes"},{"last_name":"Deblais","full_name":"Deblais, Antoine","first_name":"Antoine"},{"first_name":"Daniel","full_name":"Bonn, Daniel","last_name":"Bonn"},{"last_name":"Sommerdijk","full_name":"Sommerdijk, Nico","first_name":"Nico"},{"orcid":"0000-0002-7854-2139","first_name":"Anđela","full_name":"Šarić, Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","last_name":"Šarić"},{"full_name":"Ilie, Ioana M.","last_name":"Ilie","first_name":"Ioana M."},{"first_name":"Gijsje H.","full_name":"Koenderink, Gijsje H.","last_name":"Koenderink"},{"last_name":"Woutersen","full_name":"Woutersen, Sander","first_name":"Sander"}],"researchdata_availability":"yes","doi":"10.1073/pnas.2313162121","quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2026-09-24T10:42:11Z","das_tickbox":"1","title":"Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration","oa":1,"related_material":{"record":[{"id":"15126","relation":"research_data","status":"public"}]},"day":"12","article_type":"original","volume":121,"acknowledgement":"We thank Dr. Steven Roeters (Aarhus University), Dr. Federica Burla, and Prof. Dr. Mischa Bonn (Institute for Polymer Research, Mainz, Germany) for the useful discussions. We thank Dr. Wim Roeterdink and Michiel Hilberts for technical support. G.H.K. acknowledges financial support by the “BaSyC Building a Synthetic Cell” Gravitation grant (024.003.019) of The Netherlands Ministry of Education, Culture and Science (OCW) and The Netherlands Organization for Scientific Research and from NWO grant OCENW.GROOT.2019.022. This work has received support from the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT, under Grant No. 2022K1A3A1A04062969. This publication is part of the project (with Project Number VI.Veni.212.240) of the research programme NWO Talent Programme Veni 2021, which is financed by the Dutch Research Council (NWO). I.M.I. acknowledges support from the Sectorplan Bèta & Techniek of the Dutch Government and the Dementia Research - Synapsis Foundation Switzerland. A.Š. and K.K. acknowledge support from Royal Society and European Research Council Starting Grant. G. Giubertoni kindly thanks to the Care4Bones community and the Collagen Café community for reminding that we do not own the knowledge we create, but it is, rather, a collective resource intended for the advancement of human progress.","date_created":"2024-03-17T23:00:57Z","pmid":1,"type":"journal_article","supplementarymaterial":"no","ddc":["550"],"month":"03","dataavailabilitystatement":"Turbidity data; IR and 2D-IR data; TEM images; cryo-TEM images; CRM images; coarse grained simulations; molecular dynamics simulation data have been deposited in UvAauas.figshare 10.21942/uva.24829896","file":[{"date_created":"2024-03-19T10:22:42Z","access_level":"open_access","date_updated":"2024-03-19T10:22:42Z","success":1,"creator":"dernst","file_name":"2024_PNAS_Giubertoni.pdf","relation":"main_file","content_type":"application/pdf","checksum":"a3f7fdc29dd9f0a38952ab4e322b3a05","file_id":"15125","file_size":12952586}],"language":[{"iso":"eng"}],"department":[{"_id":"AnSa"}],"has_accepted_license":"1","scopus_import":"1","file_date_updated":"2024-03-19T10:22:42Z","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"publisher":"National Academy of Sciences"},{"fulldoi":"https://doi.org/10.1073/pnas.2409407121","isi":1,"oa_version":"Published Version","project":[{"call_identifier":"H2020","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines","grant_number":"802960"}],"date_published":"2024-10-29T00:00:00Z","issue":"44","publication_status":"published","author":[{"first_name":"Ethan","last_name":"Weiner","full_name":"Weiner, Ethan"},{"first_name":"Elizabeth","last_name":"Berryman","full_name":"Berryman, Elizabeth"},{"last_name":"Frey","id":"a0270b37-8f1a-11ec-95c7-8e710c59a4f3","full_name":"Frey, Felix F","first_name":"Felix F","orcid":"0000-0001-8501-6017"},{"first_name":"Ariadna González","full_name":"Solís, Ariadna González","last_name":"Solís"},{"full_name":"Leier, André","last_name":"Leier","first_name":"André"},{"last_name":"Lago","full_name":"Lago, Tatiana Marquez","first_name":"Tatiana Marquez"},{"orcid":"0000-0002-7854-2139","first_name":"Anđela","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","full_name":"Šarić, Anđela","last_name":"Šarić"},{"first_name":"Marisa S.","full_name":"Otegui, Marisa S.","last_name":"Otegui"}],"intvolume":"       121","abstract":[{"lang":"eng","text":"Multivesicular endosomes (MVEs) sequester membrane proteins destined for degradation within intralumenal vesicles (ILVs), a process mediated by the membrane-remodeling action of Endosomal Sorting Complex Required for Transport (ESCRT) proteins. In Arabidopsis, endosomal membrane constriction and scission are uncoupled, resulting in the formation of extensive concatenated ILV networks and enhancing cargo sequestration efficiency. Here, we used a combination of electron tomography, computer simulations, and mathematical modeling to address the questions of when concatenated ILV networks evolved in plants and what drives their formation. Through morphometric analyses of tomographic reconstructions of endosomes across yeast, algae, and various land plants, we have found that ILV concatenation is widespread within plant species, but only prevalent in seed plants, especially in flowering plants. Multiple budding sites that require the formation of pores in the limiting membrane were only identified in hornworts and seed plants, suggesting that this mechanism has evolved independently in both plant lineages. To identify the conditions under which these multiple budding sites can arise, we used particle-based molecular dynamics simulations and found that changes in ESCRT filament properties, such as filament curvature and membrane binding energy, can generate the membrane shapes observed in multiple budding sites. To understand the relationship between membrane budding activity and ILV network topology, we performed computational simulations and identified a set of membrane remodeling parameters that can recapitulate our tomographic datasets."}],"researchdata_availability":"yes","doi":"10.1073/pnas.2409407121","quality_controlled":"1","year":"2024","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","_id":"18526","OA_type":"hybrid","citation":{"ama":"Weiner E, Berryman E, Frey FF, et al. Endosomal membrane budding patterns in plants. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2024;121(44). doi:<a href=\"https://doi.org/10.1073/pnas.2409407121\">10.1073/pnas.2409407121</a>","ieee":"E. Weiner <i>et al.</i>, “Endosomal membrane budding patterns in plants,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 44. National Academy of Sciences, 2024.","apa":"Weiner, E., Berryman, E., Frey, F. F., Solís, A. G., Leier, A., Lago, T. M., … Otegui, M. S. (2024). Endosomal membrane budding patterns in plants. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2409407121\">https://doi.org/10.1073/pnas.2409407121</a>","ista":"Weiner E, Berryman E, Frey FF, Solís AG, Leier A, Lago TM, Šarić A, Otegui MS. 2024. Endosomal membrane budding patterns in plants. Proceedings of the National Academy of Sciences of the United States of America. 121(44), e2409407121.","mla":"Weiner, Ethan, et al. “Endosomal Membrane Budding Patterns in Plants.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 121, no. 44, e2409407121, National Academy of Sciences, 2024, doi:<a href=\"https://doi.org/10.1073/pnas.2409407121\">10.1073/pnas.2409407121</a>.","chicago":"Weiner, Ethan, Elizabeth Berryman, Felix F Frey, Ariadna González Solís, André Leier, Tatiana Marquez Lago, Anđela Šarić, and Marisa S. Otegui. “Endosomal Membrane Budding Patterns in Plants.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2024. <a href=\"https://doi.org/10.1073/pnas.2409407121\">https://doi.org/10.1073/pnas.2409407121</a>.","short":"E. Weiner, E. Berryman, F.F. Frey, A.G. Solís, A. Leier, T.M. Lago, A. Šarić, M.S. Otegui, Proceedings of the National Academy of Sciences of the United States of America 121 (2024)."},"external_id":{"pmid":["39441629"],"isi":["001349500800007"]},"article_processing_charge":"Yes (in subscription journal)","publication":"Proceedings of the National Academy of Sciences of the United States of America","article_number":"e2409407121","month":"10","type":"journal_article","supplementarymaterial":"yes","ddc":["570"],"acknowledgement":"We would like to thank Janice Pennington for her support with electron tomography data collection, Dr. Ingrid Jordon-Thaden, director of the Botany Garden and Greenhouse of University of Wisconsin Madison, for her invaluable assistance collecting plant materials, Dr. Marie Trest for providing Chara specimens, and Dr. Nicholas Keuler for his advice on statistical analyses. We thank Charlie Hamilton for exploring the initial computational model. This work was supported by grant NSF MCB 2114603 and NIH 1S10OD026769-01 to M.S.O. F.F acknowledges support as a NOMIS Fellow from the NOMIS Foundation. A.Š. acknowledges ERC Starting Grant “NEPA” 802960.","article_type":"original","volume":121,"date_created":"2024-11-10T23:01:59Z","pmid":1,"language":[{"iso":"eng"}],"department":[{"_id":"AnSa"}],"dataavailabilitystatement":"Electron tomogram files are deposited in EMPIAR under EMPIAR-12299 (54). All other data are included in the manuscript and/or supporting information","file":[{"success":1,"date_updated":"2024-11-11T09:35:15Z","access_level":"open_access","date_created":"2024-11-11T09:35:15Z","file_name":"2024_PNAS_Weiner.pdf","creator":"dernst","content_type":"application/pdf","relation":"main_file","file_size":5268074,"file_id":"18538","checksum":"21c82d2ab58ff99b2bd0489797be42e5"}],"scopus_import":"1","OA_place":"publisher","file_date_updated":"2024-11-11T09:35:15Z","has_accepted_license":"1","publisher":"National Academy of Sciences","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"ec_funded":1,"date_updated":"2026-09-24T10:41:24Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"title":"Endosomal membrane budding patterns in plants","das_tickbox":"1","oa":1,"day":"29"},{"publisher":"Copernicus Publications","publication_identifier":{"issn":["2699-0016"]},"file_date_updated":"2024-05-22T07:01:15Z","OA_place":"publisher","scopus_import":"1","corr_author":"1","acknowledged_ssus":[{"_id":"NMR"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"department":[{"_id":"PaSc"}],"file":[{"checksum":"80ea50114e428461ca9530d3bd5d89e4","file_size":6657865,"file_id":"15413","relation":"main_file","content_type":"application/pdf","creator":"dernst","file_name":"2024_MagneticResonance_Napoli.pdf","date_created":"2024-05-22T07:01:15Z","access_level":"open_access","date_updated":"2024-05-22T07:01:15Z","success":1}],"month":"04","type":"journal_article","ddc":["530"],"acknowledgement":"We thank Dominique Madern (IBS Grenoble) for providing the plasmid for MalDH and feedback on the article, Alicia Vallet for excellent support at the Grenoble NMR facility, and Petra Rovo and Margarita Valhondo at the IST Austria NMR Service Unit. We thank Dorothea Anrather in the mass spectrometry facility of Max Perutz Labs for the mass spectrometry analysis using the instruments of the Vienna BioCenter Core Facilities (VBCF). We are grateful to Jean-Pierre Andrieu (Plateforme Seq3A, IBS Grenoble) for the analysis of the amino acid composition of the in-house-prepared lysates. We are grateful to Rasmus Linser (Technical University Dortmund) for sharing a paper draft describing a similar study. This work was supported by the Austrian Science Fund (FWF; project number I5812-B). We thank Tobias Schubeis (Lyon) and the reviewers for constructive input.\r\nThis research has been supported by the Austrian Science Fund (grant no. I5812-B). Part of this work used the platforms of the Grenoble Instruct-ERIC center (ISBG; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for 40 Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). IBS acknowledges integration into the Interdisciplinary Research Institute of Grenoble (IRIG, 45 CEA). Charles-Adrien Arnaud was funded by GRAL.","volume":5,"article_type":"original","date_created":"2024-05-16T15:02:43Z","pmid":1,"page":"33-49","day":"19","related_material":{"record":[{"id":"22940","relation":"research_data","status":"public"}]},"oa":1,"title":"Deuteration of proteins boosted by cell lysates: High-resolution amide and Ha magic-angle-spinning (MAS) NMR without the reprotonation bottleneck","APC_amount":"1530 EUR","date_updated":"2026-09-24T10:36:36Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"doi":"10.5194/mr-5-33-2024","quality_controlled":"1","publication_status":"published","issue":"1","author":[{"first_name":"Federico","orcid":"0000-0002-9043-136X","last_name":"Napoli","id":"d42e08e7-f4fc-11eb-af0a-d71e26138f1b","full_name":"Napoli, Federico"},{"first_name":"Jia-Ying","last_name":"Guan","full_name":"Guan, Jia-Ying"},{"first_name":"Charles-Adrien","full_name":"Arnaud, Charles-Adrien","last_name":"Arnaud"},{"first_name":"Pavel","last_name":"Macek","full_name":"Macek, Pavel"},{"first_name":"Hugo","full_name":"Fraga, Hugo","last_name":"Fraga"},{"full_name":"Breyton, Cécile","last_name":"Breyton","first_name":"Cécile"},{"orcid":"0000-0002-9350-7606","first_name":"Paul","full_name":"Schanda, Paul","id":"7B541462-FAF6-11E9-A490-E8DFE5697425","last_name":"Schanda"}],"abstract":[{"lang":"eng","text":"Amide-proton-detected magic-angle-spinning NMR of deuterated proteins has become a main technique in NMR-based structural biology. In standard deuteration protocols that rely on D2O-based culture media, non-exchangeable amide sites remain deuterated, making these sites unobservable. Here we demonstrate that proteins produced with a H2O-based culture medium doped with deuterated cell lysate allow scientists to overcome this “reprotonation bottleneck” while retaining a high level of deuteration (ca. 80 %) and narrow linewidths. We quantified coherence lifetimes of several proteins prepared with this labeling pattern over a range of magic-angle-spinning (MAS) frequencies (40–100 kHz). We demonstrate that under commonly used conditions (50–60 kHz MAS), the amide 1H linewidths with our labeling approach are comparable to those of perdeuterated proteins and better than those of protonated samples at 100 kHz. For three proteins in the 33–50 kDa size range, many previously unobserved amides become visible. We report how to prepare the deuterated cell lysate for our approach from fractions of perdeuterated cultures which are usually discarded, and we show that such media can be used identically to commercial media. The residual protonation of Hα sites allows for well-resolved Hα-detected spectra and Hα resonance assignment, exemplified by the de novo assignment of 168 Hα sites in a 39 kDa protein. The approach based on this H2O/cell-lysate deuteration and MAS frequencies compatible with 1.3 or 1.9 mm rotors presents a strong sensitivity benefit over 0.7 mm 100 kHz MAS experiments."}],"intvolume":"         5","fulldoi":"https://doi.org/10.5194/mr-5-33-2024","oa_version":"Published Version","project":[{"_id":"eb9c82eb-77a9-11ec-83b8-aadd536561cf","name":"AlloSpace. The emergence and mechanisms of allostery","grant_number":"I05812"},{"name":"FWF Open Access Fund","call_identifier":"FWF","_id":"3AC91DDA-15DF-11EA-824D-93A3E7B544D1"}],"date_published":"2024-04-19T00:00:00Z","publication":"Magnetic Resonance","citation":{"short":"F. Napoli, J.-Y. Guan, C.-A. Arnaud, P. Macek, H. Fraga, C. Breyton, P. Schanda, Magnetic Resonance 5 (2024) 33–49.","chicago":"Napoli, Federico, Jia-Ying Guan, Charles-Adrien Arnaud, Pavel Macek, Hugo Fraga, Cécile Breyton, and Paul Schanda. “Deuteration of Proteins Boosted by Cell Lysates: High-Resolution Amide and Ha Magic-Angle-Spinning (MAS) NMR without the Reprotonation Bottleneck.” <i>Magnetic Resonance</i>. Copernicus Publications, 2024. <a href=\"https://doi.org/10.5194/mr-5-33-2024\">https://doi.org/10.5194/mr-5-33-2024</a>.","mla":"Napoli, Federico, et al. “Deuteration of Proteins Boosted by Cell Lysates: High-Resolution Amide and Ha Magic-Angle-Spinning (MAS) NMR without the Reprotonation Bottleneck.” <i>Magnetic Resonance</i>, vol. 5, no. 1, Copernicus Publications, 2024, pp. 33–49, doi:<a href=\"https://doi.org/10.5194/mr-5-33-2024\">10.5194/mr-5-33-2024</a>.","ista":"Napoli F, Guan J-Y, Arnaud C-A, Macek P, Fraga H, Breyton C, Schanda P. 2024. Deuteration of proteins boosted by cell lysates: High-resolution amide and Ha magic-angle-spinning (MAS) NMR without the reprotonation bottleneck. Magnetic Resonance. 5(1), 33–49.","apa":"Napoli, F., Guan, J.-Y., Arnaud, C.-A., Macek, P., Fraga, H., Breyton, C., &#38; Schanda, P. (2024). Deuteration of proteins boosted by cell lysates: High-resolution amide and Ha magic-angle-spinning (MAS) NMR without the reprotonation bottleneck. <i>Magnetic Resonance</i>. Copernicus Publications. <a href=\"https://doi.org/10.5194/mr-5-33-2024\">https://doi.org/10.5194/mr-5-33-2024</a>","ieee":"F. Napoli <i>et al.</i>, “Deuteration of proteins boosted by cell lysates: High-resolution amide and Ha magic-angle-spinning (MAS) NMR without the reprotonation bottleneck,” <i>Magnetic Resonance</i>, vol. 5, no. 1. Copernicus Publications, pp. 33–49, 2024.","ama":"Napoli F, Guan J-Y, Arnaud C-A, et al. Deuteration of proteins boosted by cell lysates: High-resolution amide and Ha magic-angle-spinning (MAS) NMR without the reprotonation bottleneck. <i>Magnetic Resonance</i>. 2024;5(1):33-49. doi:<a href=\"https://doi.org/10.5194/mr-5-33-2024\">10.5194/mr-5-33-2024</a>"},"external_id":{"pmid":["40384771"]},"article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","_id":"15401","OA_type":"gold","year":"2024"}]