[{"file_date_updated":"2024-01-22T13:41:41Z","intvolume":"       187","oa_version":"Published Version","publication":"Cell","date_updated":"2026-10-15T08:06:16Z","file":[{"file_name":"2024_Cell_Kuhn.pdf","creator":"dernst","success":1,"file_id":"14874","date_updated":"2024-01-22T13:41:41Z","date_created":"2024-01-22T13:41:41Z","content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"06fd236a9ee0b46ccb05f44695bfc34b","file_size":13194060}],"isi":1,"oa":1,"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"19395"}]},"das_tickbox":"1","department":[{"_id":"JiFr"}],"article_type":"original","date_created":"2024-01-17T12:45:40Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","dataavailabilitystatement":"Phosphoproteome data are available in the ProteomeXchange PRIDE database: PXD038369 and PXD038363. Transcriptome data are available in the NCBI Sequence Read Archive: PRJNA881051. Data underlying RAF-like kinase phylogeny is deposited at the iTOL: https://itol.embl.de/shared/dolfweijers. This paper analyzes existing, publicly available data phospho-proteome data. The accession number for the datasets are listed in the key resources table.\r\n\r\nCode for the AuxPhos app and for MATlab code for analysis of cytoplasmic streaming are available at Github: https://github.com/WeijersLab/AuxPhos and https://github.com/jorissprakel/RAF_track_analysis.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead author upon request.","month":"01","ec_funded":1,"_id":"14826","status":"public","issue":"1","project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"RNA-directed DNA methylation in plant development","grant_number":"P29988","_id":"262EF96E-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"publisher":"Elsevier","quality_controlled":"1","title":"RAF-like protein kinases mediate a deeply conserved, rapid auxin response","abstract":[{"lang":"eng","text":"The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants and algae. The nuclear auxin pathway mediates gene expression and controls growth and development in land plants, but this pathway is absent from algal sister groups. Several components of rapid responses have been identified in Arabidopsis, but it is unknown if these are part of a conserved mechanism. We recently identified a fast, proteome-wide phosphorylation response to auxin. Here, we show that this response occurs across 5 land plant and algal species and converges on a core group of shared targets. We found conserved rapid physiological responses to auxin in the same species and identified rapidly accelerated fibrosarcoma (RAF)-like protein kinases as central mediators of auxin-triggered phosphorylation across species. Genetic analysis connects this kinase to both auxin-triggered protein phosphorylation and rapid cellular response, thus identifying an ancient mechanism for fast auxin responses in the green lineage."}],"acknowledgement":"We are grateful to Asuka Shitaku and Eri Koide for generating and sharing the Marchantia PRAF-mCitrine line and Peng-Cheng Wang for sharing the Arabidopsis raf mutant. We are grateful to our team members for discussions and helpful advice. This work was supported by funding from the Netherlands Organization for Scientific Research (NWO): VICI grant 865.14.001 and ENW-KLEIN OCENW.KLEIN.027 grants to D.W.; VENI grant VI.VENI.212.003 to A.K.; the European Research Council AdG DIRNDL (contract number 833867) to D.W.; CoG CATCH to J.S.; StG CELLONGATE (contract 803048) to M.F.; and AdG ETAP (contract 742985) to J.F.; MEXT KAKENHI grant number JP19H05675 to T.K.; JSPS KAKENHI grant number JP20H03275 to R.N.; Takeda Science Foundation to R.N.; and the Austrian Science Fund (FWF, P29988) to J.F.","researchdata_availability":"yes","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"year":"2024","volume":187,"publication_status":"published","citation":{"ista":"Kuhn A, Roosjen M, Mutte S, Dubey SM, Carrillo Carrasco VP, Boeren S, Monzer A, Koehorst J, Kohchi T, Nishihama R, Fendrych M, Sprakel J, Friml J, Weijers D. 2024. RAF-like protein kinases mediate a deeply conserved, rapid auxin response. Cell. 187(1), 130–148.e17.","mla":"Kuhn, Andre, et al. “RAF-like Protein Kinases Mediate a Deeply Conserved, Rapid Auxin Response.” <i>Cell</i>, vol. 187, no. 1, Elsevier, 2024, p. 130–148.e17, doi:<a href=\"https://doi.org/10.1016/j.cell.2023.11.021\">10.1016/j.cell.2023.11.021</a>.","ama":"Kuhn A, Roosjen M, Mutte S, et al. RAF-like protein kinases mediate a deeply conserved, rapid auxin response. <i>Cell</i>. 2024;187(1):130-148.e17. doi:<a href=\"https://doi.org/10.1016/j.cell.2023.11.021\">10.1016/j.cell.2023.11.021</a>","apa":"Kuhn, A., Roosjen, M., Mutte, S., Dubey, S. M., Carrillo Carrasco, V. P., Boeren, S., … Weijers, D. (2024). RAF-like protein kinases mediate a deeply conserved, rapid auxin response. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2023.11.021\">https://doi.org/10.1016/j.cell.2023.11.021</a>","chicago":"Kuhn, Andre, Mark Roosjen, Sumanth Mutte, Shiv Mani Dubey, Vanessa Polet Carrillo Carrasco, Sjef Boeren, Aline Monzer, et al. “RAF-like Protein Kinases Mediate a Deeply Conserved, Rapid Auxin Response.” <i>Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cell.2023.11.021\">https://doi.org/10.1016/j.cell.2023.11.021</a>.","ieee":"A. Kuhn <i>et al.</i>, “RAF-like protein kinases mediate a deeply conserved, rapid auxin response,” <i>Cell</i>, vol. 187, no. 1. Elsevier, p. 130–148.e17, 2024.","short":"A. Kuhn, M. Roosjen, S. Mutte, S.M. Dubey, V.P. Carrillo Carrasco, S. Boeren, A. Monzer, J. Koehorst, T. Kohchi, R. Nishihama, M. Fendrych, J. Sprakel, J. Friml, D. Weijers, Cell 187 (2024) 130–148.e17."},"doi":"10.1016/j.cell.2023.11.021","external_id":{"isi":["001152705700001"],"pmid":["38128538"]},"pmid":1,"date_published":"2024-01-04T00:00:00Z","language":[{"iso":"eng"}],"keyword":["General Biochemistry","Genetics and Molecular Biology"],"ddc":["580"],"fulldoi":"https://doi.org/10.1016/j.cell.2023.11.021","has_accepted_license":"1","day":"04","article_processing_charge":"Yes (in subscription journal)","type":"journal_article","supplementarymaterial":"yes","page":"130-148.e17","author":[{"last_name":"Kuhn","first_name":"Andre","full_name":"Kuhn, Andre"},{"full_name":"Roosjen, Mark","first_name":"Mark","last_name":"Roosjen"},{"last_name":"Mutte","full_name":"Mutte, Sumanth","first_name":"Sumanth"},{"first_name":"Shiv Mani","full_name":"Dubey, Shiv Mani","last_name":"Dubey"},{"last_name":"Carrillo Carrasco","full_name":"Carrillo Carrasco, Vanessa Polet","first_name":"Vanessa Polet"},{"full_name":"Boeren, Sjef","first_name":"Sjef","last_name":"Boeren"},{"full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","first_name":"Aline","last_name":"Monzer"},{"first_name":"Jasper","full_name":"Koehorst, Jasper","last_name":"Koehorst"},{"last_name":"Kohchi","full_name":"Kohchi, Takayuki","first_name":"Takayuki"},{"first_name":"Ryuichi","full_name":"Nishihama, Ryuichi","last_name":"Nishihama"},{"first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas","orcid":"0000-0002-9767-8699","last_name":"Fendrych"},{"full_name":"Sprakel, Joris","first_name":"Joris","last_name":"Sprakel"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"},{"last_name":"Weijers","full_name":"Weijers, Dolf","first_name":"Dolf"}],"publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"scopus_import":"1"},{"fulldoi":"https://doi.org/10.1093/plcell/koad319","ddc":["580"],"has_accepted_license":"1","language":[{"iso":"eng"}],"external_id":{"pmid":["38124479"]},"date_published":"2024-04-01T00:00:00Z","pmid":1,"author":[{"first_name":"Jan W.","full_name":"Huebbers, Jan W.","last_name":"Huebbers"},{"first_name":"George A.","full_name":"Caldarescu, George A.","last_name":"Caldarescu"},{"last_name":"Kubátová","first_name":"Zdeňka","full_name":"Kubátová, Zdeňka"},{"full_name":"Sabol, Peter","first_name":"Peter","last_name":"Sabol"},{"last_name":"Levecque","first_name":"Sophie C.J.","full_name":"Levecque, Sophie C.J."},{"full_name":"Kuhn, Hannah","first_name":"Hannah","last_name":"Kuhn"},{"full_name":"Kulich, Ivan","first_name":"Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","last_name":"Kulich"},{"last_name":"Reinstädler","first_name":"Anja","full_name":"Reinstädler, Anja"},{"first_name":"Kim","full_name":"Büttgen, Kim","last_name":"Büttgen"},{"first_name":"Alba","full_name":"Manga-Robles, Alba","last_name":"Manga-Robles"},{"first_name":"Hugo","full_name":"Mélida, Hugo","last_name":"Mélida"},{"full_name":"Pauly, Markus","first_name":"Markus","last_name":"Pauly"},{"last_name":"Panstruga","full_name":"Panstruga, Ralph","first_name":"Ralph"},{"last_name":"Žárský","full_name":"Žárský, Viktor","first_name":"Viktor"}],"scopus_import":"1","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"supplementarymaterial":"yes","page":"1007-1035","article_processing_charge":"Yes (in subscription journal)","type":"journal_article","day":"01","month":"04","dataavailabilitystatement":"All relevant data can be found within the manuscript and its supporting materials.","_id":"15319","article_type":"original","department":[{"_id":"JiFr"}],"date_created":"2024-04-14T22:01:02Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa":1,"date_updated":"2026-10-15T08:09:23Z","file":[{"access_level":"open_access","content_type":"application/pdf","date_created":"2024-04-17T06:57:54Z","checksum":"f9994d2e4748feec24c992b39871aba1","file_size":2866098,"relation":"main_file","creator":"dernst","file_name":"2024_PlantCell_Huebbers.pdf","date_updated":"2024-04-17T06:57:54Z","file_id":"15326","success":1}],"das_tickbox":"1","intvolume":"        36","file_date_updated":"2024-04-17T06:57:54Z","oa_version":"Published Version","publication":"Plant Cell","doi":"10.1093/plcell/koad319","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"volume":36,"year":"2024","researchdata_availability":"yes (supplementary data)","citation":{"apa":"Huebbers, J. W., Caldarescu, G. A., Kubátová, Z., Sabol, P., Levecque, S. C. J., Kuhn, H., … Žárský, V. (2024). Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. <i>Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koad319\">https://doi.org/10.1093/plcell/koad319</a>","chicago":"Huebbers, Jan W., George A. Caldarescu, Zdeňka Kubátová, Peter Sabol, Sophie C.J. Levecque, Hannah Kuhn, Ivan Kulich, et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” <i>Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koad319\">https://doi.org/10.1093/plcell/koad319</a>.","ieee":"J. W. Huebbers <i>et al.</i>, “Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew,” <i>Plant Cell</i>, vol. 36, no. 4. Oxford University Press, pp. 1007–1035, 2024.","short":"J.W. Huebbers, G.A. Caldarescu, Z. Kubátová, P. Sabol, S.C.J. Levecque, H. Kuhn, I. Kulich, A. Reinstädler, K. Büttgen, A. Manga-Robles, H. Mélida, M. Pauly, R. Panstruga, V. Žárský, Plant Cell 36 (2024) 1007–1035.","ista":"Huebbers JW, Caldarescu GA, Kubátová Z, Sabol P, Levecque SCJ, Kuhn H, Kulich I, Reinstädler A, Büttgen K, Manga-Robles A, Mélida H, Pauly M, Panstruga R, Žárský V. 2024. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. Plant Cell. 36(4), 1007–1035.","mla":"Huebbers, Jan W., et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” <i>Plant Cell</i>, vol. 36, no. 4, Oxford University Press, 2024, pp. 1007–35, doi:<a href=\"https://doi.org/10.1093/plcell/koad319\">10.1093/plcell/koad319</a>.","ama":"Huebbers JW, Caldarescu GA, Kubátová Z, et al. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. <i>Plant Cell</i>. 2024;36(4):1007-1035. doi:<a href=\"https://doi.org/10.1093/plcell/koad319\">10.1093/plcell/koad319</a>"},"publication_status":"published","title":"Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew","abstract":[{"lang":"eng","text":"Exocyst component of 70-kDa (EXO70) proteins are constituents of the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) proteins are plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap of Arabidopsis thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants regarding the biogenesis of leaf trichome secondary cell walls. Biochemical and Fourier transform infrared spectroscopic analyses corroborated deficiencies in the composition of trichome cell walls in these mutants. Transgenic lines expressing fluorophore-tagged EXO70H4 and MLO exhibited extensive colocalization of these proteins. Furthermore, mCherry-EXO70H4 mislocalized in trichomes of the mlo triple mutant and, vice versa, MLO6-GFP mislocalized in trichomes of the exo70H4 mutant. Expression of GFP-marked PMR4 callose synthase, a known cargo of EXO70H4-dependent exocytosis, revealed reduced cell wall delivery of GFP-PMR4 in trichomes of mlo triple mutant plants. In vivo protein–protein interaction assays in plant and yeast cells uncovered isoform-preferential interactions between EXO70.2 subfamily members and MLO proteins. Finally, exo70H4 and mlo6 mutants, when combined, showed synergistically enhanced resistance to powdery mildew attack. Taken together, our data point to an isoform-specific interplay of EXO70 and MLO proteins in the modulation of trichome cell wall biogenesis and powdery mildew susceptibility."}],"publisher":"Oxford University Press","issue":"4","status":"public","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","quality_controlled":"1"},{"language":[{"iso":"eng"}],"pmid":1,"external_id":{"pmid":["38988072"]},"date_published":"2024-11-11T00:00:00Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.xplc.2024.101039","ddc":["580"],"type":"journal_article","article_processing_charge":"Yes","day":"11","publication_identifier":{"eissn":["2590-3462"]},"scopus_import":"1","author":[{"last_name":"Das","full_name":"Das, Shubhajit","id":"b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be","first_name":"Shubhajit"},{"first_name":"Martijn","full_name":"De Roij, Martijn","last_name":"De Roij"},{"last_name":"Bellows","first_name":"Simon","full_name":"Bellows, Simon"},{"first_name":"Melissa Dipp","full_name":"Alvarez, Melissa Dipp","last_name":"Alvarez"},{"last_name":"Mutte","full_name":"Mutte, Sumanth","first_name":"Sumanth"},{"last_name":"Kohlen","full_name":"Kohlen, Wouter","first_name":"Wouter"},{"full_name":"Farcot, Etienne","first_name":"Etienne","last_name":"Farcot"},{"full_name":"Weijers, Dolf","first_name":"Dolf","last_name":"Weijers"},{"full_name":"Borst, Jan Willem","first_name":"Jan Willem","last_name":"Borst"}],"article_number":"101039","supplementarymaterial":"yes","das_tickbox":"0","oa":1,"file":[{"file_id":"18811","date_updated":"2025-01-09T12:25:32Z","success":1,"file_name":"2024_PlantCommunications_Das.pdf","creator":"dernst","file_size":4970540,"checksum":"38cabc1042ac7fb70e6c4c510eba88fc","relation":"main_file","access_level":"open_access","content_type":"application/pdf","date_created":"2025-01-09T12:25:32Z"}],"date_updated":"2026-10-15T08:12:01Z","oa_version":"Published Version","publication":"Plant Communications","intvolume":"         5","file_date_updated":"2025-01-09T12:25:32Z","DOAJ_listed":"1","_id":"17436","month":"11","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-08-18T22:01:04Z","article_type":"original","department":[{"_id":"JiFr"}],"acknowledgement":"We are grateful to Iris Nieuwland and Neri van Laar for experimental support. No conflict of interest declared.\r\nThis work was supported by the Netherlands Organisation for Scientific Research, the Netherlands (grants ALWOP.402 and OCENW.M20.031 to J.W.B.) and the Human Frontiers Research Program (grant RGP0015/2022 to D.W.).","abstract":[{"text":"The auxin signaling molecule controls a variety of growth and developmental processes in land plants. Auxin regulates gene expression through a nuclear auxin signaling pathway (NAP) consisting of the ubiquitin ligase auxin receptor TIR1/AFB, its Aux/IAA degradation substrate, and DNA-binding ARF transcription factors. Although extensive qualitative understanding of the pathway and its interactions has been obtained, mostly by studying the flowering plant Arabidopsis thaliana, it remains unknown how these translate to quantitative system behavior in vivo, a problem that is confounded by the large NAP gene families in most species. Here, we used the minimal NAP of the liverwort Marchantia polymorpha to quantitatively map NAP protein accumulation and dynamics in vivo through the use of knockin fluorescent fusion proteins. Beyond revealing the dynamic native accumulation profile of the entire NAP protein network, we discovered that the two central ARFs, MpARF1 and MpARF2, are proteasomally degraded. This auxin-independent degradation tunes ARF protein stoichiometry to favor gene activation, thereby reprogramming auxin response during the developmental progression. Thus, quantitative analysis of the entire NAP has enabled us to identify ARF degradation and the stoichiometries of activator and repressor ARFs as a potential mechanism for controlling gemma germination.","lang":"eng"}],"title":"Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination","quality_controlled":"1","publisher":"Elsevier","issue":"11","status":"public","doi":"10.1016/j.xplc.2024.101039","OA_type":"gold","OA_place":"publisher","citation":{"ama":"Das S, De Roij M, Bellows S, et al. Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination. <i>Plant Communications</i>. 2024;5(11). doi:<a href=\"https://doi.org/10.1016/j.xplc.2024.101039\">10.1016/j.xplc.2024.101039</a>","mla":"Das, Shubhajit, et al. “Quantitative Imaging Reveals the Role of MpARF Proteasomal Degradation during Gemma Germination.” <i>Plant Communications</i>, vol. 5, no. 11, 101039, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.xplc.2024.101039\">10.1016/j.xplc.2024.101039</a>.","ista":"Das S, De Roij M, Bellows S, Alvarez MD, Mutte S, Kohlen W, Farcot E, Weijers D, Borst JW. 2024. Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination. Plant Communications. 5(11), 101039.","short":"S. Das, M. De Roij, S. Bellows, M.D. Alvarez, S. Mutte, W. Kohlen, E. Farcot, D. Weijers, J.W. Borst, Plant Communications 5 (2024).","ieee":"S. Das <i>et al.</i>, “Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination,” <i>Plant Communications</i>, vol. 5, no. 11. Elsevier, 2024.","chicago":"Das, Shubhajit, Martijn De Roij, Simon Bellows, Melissa Dipp Alvarez, Sumanth Mutte, Wouter Kohlen, Etienne Farcot, Dolf Weijers, and Jan Willem Borst. “Quantitative Imaging Reveals the Role of MpARF Proteasomal Degradation during Gemma Germination.” <i>Plant Communications</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.xplc.2024.101039\">https://doi.org/10.1016/j.xplc.2024.101039</a>.","apa":"Das, S., De Roij, M., Bellows, S., Alvarez, M. D., Mutte, S., Kohlen, W., … Borst, J. W. (2024). Quantitative imaging reveals the role of MpARF proteasomal degradation during gemma germination. <i>Plant Communications</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.xplc.2024.101039\">https://doi.org/10.1016/j.xplc.2024.101039</a>"},"publication_status":"published","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"researchdata_availability":"no","volume":5,"year":"2024"},{"doi":"10.1016/j.devcel.2024.09.020","OA_type":"hybrid","citation":{"mla":"Janacek, DP, et al. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” <i>Developmental Cell</i>, vol. 59, no. 14, Elsevier, 2024, pp. S1534-5807(24)00569-0, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">10.1016/j.devcel.2024.09.020</a>.","ama":"Janacek D, Kolb M, Schulz L, et al. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. <i>Developmental Cell</i>. 2024;59(14):S1534-5807(24)00569-0. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">10.1016/j.devcel.2024.09.020</a>","ista":"Janacek D, Kolb M, Schulz L, Mergner J, Kuster B, Glanc M, Friml J, Ten Tusscher K, Schwechheimer C, Hammes U. 2024. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. 59(14), S1534-5807(24)00569–0.","ieee":"D. Janacek <i>et al.</i>, “Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport,” <i>Developmental Cell</i>, vol. 59, no. 14. Elsevier, pp. S1534-5807(24)00569–0, 2024.","short":"D. Janacek, M. Kolb, L. Schulz, J. Mergner, B. Kuster, M. Glanc, J. Friml, K. Ten Tusscher, C. Schwechheimer, U. Hammes, Developmental Cell 59 (2024) S1534-5807(24)00569–0.","apa":"Janacek, D., Kolb, M., Schulz, L., Mergner, J., Kuster, B., Glanc, M., … Hammes, U. (2024). Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">https://doi.org/10.1016/j.devcel.2024.09.020</a>","chicago":"Janacek, DP, M Kolb, L Schulz, J Mergner, B Kuster, Matous Glanc, Jiří Friml, K Ten Tusscher, C Schwechheimer, and UZ Hammes. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">https://doi.org/10.1016/j.devcel.2024.09.020</a>."},"OA_place":"publisher","publication_status":"published","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"researchdata_availability":"yes","year":"2024","volume":59,"acknowledgement":"This work was funded by DFG3468/6-1, DFG3468/6-3, and SFB924 to U.Z.H. We thank Angela Alkofer and Helene Prunkl for excellent technical assistance and Xenopus maintenance. Christian Luschnig is acknowledged for sharing unpublished results and valuable discussions.","title":"Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport","abstract":[{"lang":"eng","text":"The phytohormone auxin is polarly transported in plants by PIN-FORMED (PIN) transporters and controls virtually all growth and developmental processes. Canonical PINs possess a long, largely disordered cytosolic loop. Auxin transport by canonical PINs is activated by loop phosphorylation by certain kinases. The structure of the PIN transmembrane domains was recently determined, their transport properties remained poorly characterized, and the role of the loop in the transport process was unclear. Here, we determined the quantitative kinetic parameters of auxin transport mediated by Arabidopsis PINs to mathematically model auxin distribution in roots and to test these predictions in vivo. Using chimeras between transmembrane and loop domains of different PINs, we demonstrate a strong correlation between transport parameters and physiological output, indicating that the loop domain is not only required to activate PIN-mediated auxin transport, but it has an additional role in the transport process by a currently unknown mechanism."}],"quality_controlled":"1","publisher":"Elsevier","status":"public","issue":"14","_id":"18465","month":"12","dataavailabilitystatement":"The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD044850 (user name: reviewer_pxd044850@ebi.ac.uk, password: 3xBdgLSX).59\r\n\r\nThis paper does not report original codes.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","date_created":"2024-10-23T08:41:27Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","department":[{"_id":"JiFr"}],"das_tickbox":"1","oa":1,"date_updated":"2026-10-15T08:13:11Z","file":[{"checksum":"34423ee9fb4e30334f3572eddf1da2ae","file_size":3675955,"relation":"main_file","access_level":"open_access","date_created":"2025-01-13T09:20:15Z","content_type":"application/pdf","success":1,"date_updated":"2025-01-13T09:20:15Z","file_id":"18835","file_name":"2024_DevelopmentalCell_Janacek.pdf","creator":"dernst"}],"isi":1,"publication":"Developmental Cell","oa_version":"Published Version","intvolume":"        59","file_date_updated":"2025-01-13T09:20:15Z","scopus_import":"1","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"author":[{"first_name":"DP","full_name":"Janacek, DP","last_name":"Janacek"},{"first_name":"M","full_name":"Kolb, M","last_name":"Kolb"},{"first_name":"L","full_name":"Schulz, L","last_name":"Schulz"},{"full_name":"Mergner, J","first_name":"J","last_name":"Mergner"},{"last_name":"Kuster","full_name":"Kuster, B","first_name":"B"},{"orcid":"0000-0003-0619-7783","last_name":"Glanc","first_name":"Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","full_name":"Glanc, Matous"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"first_name":"K","full_name":"Ten Tusscher, K","last_name":"Ten Tusscher"},{"last_name":"Schwechheimer","first_name":"C","full_name":"Schwechheimer, C"},{"last_name":"Hammes","full_name":"Hammes, UZ","first_name":"UZ"}],"page":"S1534-5807(24)00569-0","supplementarymaterial":"yes","type":"journal_article","article_processing_charge":"Yes (in subscription journal)","day":"16","has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.devcel.2024.09.020","ddc":["570"],"language":[{"iso":"eng"}],"date_published":"2024-12-16T00:00:00Z","external_id":{"pmid":["39413780"],"isi":["001390774300001"]},"pmid":1},{"doi":"10.1038/s41467-024-54240-y","OA_type":"gold","publication_status":"published","citation":{"chicago":"Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated Plant Development.” <i>Nature Communications</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41467-024-54240-y\">https://doi.org/10.1038/s41467-024-54240-y</a>.","apa":"Luschnig, C., &#38; Friml, J. (2024). Over 25 years of decrypting PIN-mediated plant development. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-024-54240-y\">https://doi.org/10.1038/s41467-024-54240-y</a>","short":"C. Luschnig, J. Friml, Nature Communications 15 (2024).","ieee":"C. Luschnig and J. Friml, “Over 25 years of decrypting PIN-mediated plant development,” <i>Nature Communications</i>, vol. 15. Springer Nature, 2024.","ista":"Luschnig C, Friml J. 2024. Over 25 years of decrypting PIN-mediated plant development. Nature Communications. 15, 9904.","ama":"Luschnig C, Friml J. Over 25 years of decrypting PIN-mediated plant development. <i>Nature Communications</i>. 2024;15. doi:<a href=\"https://doi.org/10.1038/s41467-024-54240-y\">10.1038/s41467-024-54240-y</a>","mla":"Luschnig, Christian, and Jiří Friml. “Over 25 Years of Decrypting PIN-Mediated Plant Development.” <i>Nature Communications</i>, vol. 15, 9904, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s41467-024-54240-y\">10.1038/s41467-024-54240-y</a>."},"OA_place":"publisher","volume":15,"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","researchdata_availability":"no","acknowledgement":"We gratefully acknowledge Leo Gälweiler for authorizing his PIN1 story. We would like to thank Yuanrong Pei for invaluable help with preparing figures. Work in the lab of C.L. is supported by grants from the Austrian Science Fund (PAT 8419423) and by the Gesellschaft für Forschungsförderung Niederösterreich m.b.H. (FTI19-008). The lab of J.F. is supported by the Austrian Science Fund (I 6123-B and P 37051-B).","title":"Over 25 years of decrypting PIN-mediated plant development","abstract":[{"lang":"eng","text":"Identification of PIN exporters for auxin, the major coordinative signal in plants, some 25 years ago, signifies a landmark in our understanding of plant-specific mechanisms underlying development and adaptation. Auxin is directionally transported throughout the plant body; a unique feature already envisioned by Darwin and solidified by PINs’ discovery and characterization. The PIN-based auxin distribution network with its complex regulations of PIN expression, localization and activity turned out to underlie a remarkable multitude of developmental processes and represents means to integrate endogenous and environmental signals. Given the recent anniversary, we here summarize past and current developments in this exciting field."}],"quality_controlled":"1","status":"public","publisher":"Springer Nature","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","grant_number":"P37051","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"_id":"18582","DOAJ_listed":"1","month":"12","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-11-24T23:01:48Z","department":[{"_id":"JiFr"}],"article_type":"original","das_tickbox":"0","date_updated":"2026-10-15T08:10:07Z","isi":1,"file":[{"file_name":"2024_NatureComm_Luschnig.pdf","creator":"dernst","file_id":"18615","date_updated":"2024-12-03T14:10:54Z","success":1,"access_level":"open_access","content_type":"application/pdf","date_created":"2024-12-03T14:10:54Z","file_size":1426555,"checksum":"3a31af06f52100d287f1e9d9c2aa1d40","relation":"main_file"}],"oa":1,"oa_version":"Published Version","publication":"Nature Communications","file_date_updated":"2024-12-03T14:10:54Z","intvolume":"        15","publication_identifier":{"eissn":["2041-1723"]},"scopus_import":"1","author":[{"last_name":"Luschnig","first_name":"Christian","full_name":"Luschnig, Christian"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"corr_author":"1","article_number":"9904","supplementarymaterial":"no","type":"journal_article","article_processing_charge":"Yes","day":"01","has_accepted_license":"1","ddc":["580"],"fulldoi":"https://doi.org/10.1038/s41467-024-54240-y","language":[{"iso":"eng"}],"external_id":{"pmid":["39548100"],"isi":["001356232600004"]},"pmid":1,"date_published":"2024-12-01T00:00:00Z"},{"acknowledgement":"We thank the Cryo-EM Center of the University of Science and Technology of China for the EM facility support, Y. Yin (Iowa State University, Ames, IA, USA) for providing the anti-BES1 antibody, Y. Gao and all other staff members for their technical support on cryo-EM data collection, S. Vanneste (VIB, Ghent University, Ghent, Belgium) for useful discussions, and M. De Cock for help in preparing the manuscript.\r\nThis work was supported by the National Natural Science Foundation of China (grants 31870732 and 32322041 to L.S., grant 31900885 to X.L., and grant 32321001 to L.S.); the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB37020103 to L.S.); the Natural Science Foundation of Anhui Province (grant 2008085MC90 to X.L. and grant 2008085J15 to L.S.); Fundamental Research Funds for the Central Universities (grant WK9100000031 to L.S.); USTC Research Funds of the Double First-Class Initiative (grant YD9100002004 to L.S. and grant YD9100002020 to X.L.); Research Foundation-Flanders (grant G002121N to E.R. and postdoctoral fellowships 12R7822N and 12R7819N to N.V.); and Chinese Scholarship Council predoctoral fellowships (Y.W. and H.Z.). L.S. is supported by an Outstanding Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from the Cyrus Tang Foundation.","title":"Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export","abstract":[{"lang":"eng","text":"Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling."}],"quality_controlled":"1","publisher":"American Association for the Advancement of Science","issue":"6689","status":"public","doi":"10.1126/science.adj4591","OA_type":"green","OA_place":"repository","citation":{"chicago":"Ying, Wei, Yaowei Wang, Hong Wei, Yongming Luo, Qian Ma, Heyuan Zhu, Hilde Janssens, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” <i>Science</i>. American Association for the Advancement of Science, 2024. <a href=\"https://doi.org/10.1126/science.adj4591\">https://doi.org/10.1126/science.adj4591</a>.","apa":"Ying, W., Wang, Y., Wei, H., Luo, Y., Ma, Q., Zhu, H., … Sun, L. (2024). Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.adj4591\">https://doi.org/10.1126/science.adj4591</a>","short":"W. Ying, Y. Wang, H. Wei, Y. Luo, Q. Ma, H. Zhu, H. Janssens, N. Vukašinović, M. Kvasnica, J.M. Winne, Y. Gao, S. Tan, J. Friml, X. Liu, E. Russinova, L. Sun, Science 383 (2024) eadj4591.","ieee":"W. Ying <i>et al.</i>, “Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export,” <i>Science</i>, vol. 383, no. 6689. American Association for the Advancement of Science, p. eadj4591, 2024.","ista":"Ying W, Wang Y, Wei H, Luo Y, Ma Q, Zhu H, Janssens H, Vukašinović N, Kvasnica M, Winne JM, Gao Y, Tan S, Friml J, Liu X, Russinova E, Sun L. 2024. Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. Science. 383(6689), eadj4591.","ama":"Ying W, Wang Y, Wei H, et al. Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. <i>Science</i>. 2024;383(6689):eadj4591. doi:<a href=\"https://doi.org/10.1126/science.adj4591\">10.1126/science.adj4591</a>","mla":"Ying, Wei, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” <i>Science</i>, vol. 383, no. 6689, American Association for the Advancement of Science, 2024, p. eadj4591, doi:<a href=\"https://doi.org/10.1126/science.adj4591\">10.1126/science.adj4591</a>."},"publication_status":"published","volume":383,"year":"2024","researchdata_availability":"yes","das_tickbox":"1","oa":1,"isi":1,"date_updated":"2026-10-15T08:08:30Z","publication":"Science","oa_version":"Submitted Version","intvolume":"       383","_id":"15251","month":"03","dataavailabilitystatement":"All data are available in the main text or the supplementary materials. Model coordinates and cryo-EM density maps for the WT ABCB19 structures in the substrate-unbound state, BL-bound state, BL and AMP-PNP-bound state, and the E529Q/E1174Q mutant ABCB19 structure in the ATP-bound state have been deposited in the Protein Data Bank with accession codes 8WOI, 8WOM, 8WOO, and 8WP0, respectively, and in the Electron Microscopy Data Bank with accession codes EMD-37690, EMD-37692, EMD-37694, and EMD-37705, respectively.","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-03-31T22:01:12Z","article_type":"original","department":[{"_id":"JiFr"}],"type":"journal_article","article_processing_charge":"No","day":"22","publication_identifier":{"eissn":["1095-9203"]},"scopus_import":"1","main_file_link":[{"open_access":"1","url":"http://hdl.handle.net/1854/LU-01HTMBK3P3PPNB73YFCMFXP3ZZ"}],"author":[{"full_name":"Ying, Wei","first_name":"Wei","last_name":"Ying"},{"last_name":"Wang","first_name":"Yaowei","full_name":"Wang, Yaowei"},{"first_name":"Hong","full_name":"Wei, Hong","last_name":"Wei"},{"last_name":"Luo","full_name":"Luo, Yongming","first_name":"Yongming"},{"last_name":"Ma","full_name":"Ma, Qian","first_name":"Qian"},{"last_name":"Zhu","full_name":"Zhu, Heyuan","first_name":"Heyuan"},{"first_name":"Hilde","full_name":"Janssens, Hilde","last_name":"Janssens"},{"first_name":"Nemanja","full_name":"Vukašinović, Nemanja","last_name":"Vukašinović"},{"full_name":"Kvasnica, Miroslav","first_name":"Miroslav","last_name":"Kvasnica"},{"first_name":"Johan M.","full_name":"Winne, Johan M.","last_name":"Winne"},{"first_name":"Yongxiang","full_name":"Gao, Yongxiang","last_name":"Gao"},{"last_name":"Tan","orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"full_name":"Liu, Xin","first_name":"Xin","last_name":"Liu"},{"full_name":"Russinova, Eugenia","first_name":"Eugenia","last_name":"Russinova"},{"first_name":"Linfeng","full_name":"Sun, Linfeng","last_name":"Sun"}],"page":"eadj4591","supplementarymaterial":"yes","language":[{"iso":"eng"}],"pmid":1,"date_published":"2024-03-22T00:00:00Z","external_id":{"pmid":["38513023"],"isi":["001252955200028"]},"fulldoi":"https://doi.org/10.1126/science.adj4591"},{"month":"12","_id":"17377","department":[{"_id":"JiFr"}],"article_type":"original","date_created":"2024-08-04T22:01:22Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","isi":1,"file":[{"checksum":"89a83fe25b9ec1aad8e22d134a342c09","file_size":277636,"relation":"main_file","access_level":"open_access","content_type":"application/pdf","date_created":"2025-11-24T15:12:50Z","date_updated":"2025-11-24T15:12:50Z","file_id":"20691","success":1,"file_name":"2024_TrendsPlantScience_Wojcikowska_submittedversion.pdf","creator":"dernst"}],"date_updated":"2026-10-15T08:27:18Z","oa":1,"das_tickbox":"0","file_date_updated":"2025-11-24T15:12:50Z","intvolume":"        29","oa_version":"Submitted Version","publication":"Trends in Plant Science","OA_type":"green","doi":"10.1016/j.tplants.2024.07.008","volume":29,"researchdata_availability":"no","year":"2024","publication_status":"published","OA_place":"repository","citation":{"apa":"Wójcikowska, B., Friml, J., &#38; Mazur, E. (2024). BiAux, a newly discovered compound triggering auxin signaling. <i>Trends in Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">https://doi.org/10.1016/j.tplants.2024.07.008</a>","chicago":"Wójcikowska, Barbara, Jiří Friml, and Ewa Mazur. “BiAux, a Newly Discovered Compound Triggering Auxin Signaling.” <i>Trends in Plant Science</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">https://doi.org/10.1016/j.tplants.2024.07.008</a>.","ieee":"B. Wójcikowska, J. Friml, and E. Mazur, “BiAux, a newly discovered compound triggering auxin signaling,” <i>Trends in Plant Science</i>, vol. 29, no. 12. Elsevier, pp. 1279–1281, 2024.","short":"B. Wójcikowska, J. Friml, E. Mazur, Trends in Plant Science 29 (2024) 1279–1281.","ista":"Wójcikowska B, Friml J, Mazur E. 2024. BiAux, a newly discovered compound triggering auxin signaling. Trends in Plant Science. 29(12), 1279–1281.","mla":"Wójcikowska, Barbara, et al. “BiAux, a Newly Discovered Compound Triggering Auxin Signaling.” <i>Trends in Plant Science</i>, vol. 29, no. 12, Elsevier, 2024, pp. 1279–81, doi:<a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">10.1016/j.tplants.2024.07.008</a>.","ama":"Wójcikowska B, Friml J, Mazur E. BiAux, a newly discovered compound triggering auxin signaling. <i>Trends in Plant Science</i>. 2024;29(12):1279-1281. doi:<a href=\"https://doi.org/10.1016/j.tplants.2024.07.008\">10.1016/j.tplants.2024.07.008</a>"},"title":"BiAux, a newly discovered compound triggering auxin signaling","abstract":[{"lang":"eng","text":"Lateral root (LR) formation, that is vital for plant development, is one of many auxin-modulated processes, but the underlying regulatory mechanism is not yet fully known. Recently, \r\nGonzález-García et al. discovered the BiAux compound and showed that it is involved in LR development via regulating specific auxin coreceptors."}],"acknowledgement":"This work was supported by funding from the National Science Centre, Poland, under the OPUS call in the Weave program and the Austrian Science Fund, Austria project (FWF)/OPUS – Peptide receptor complexes for auxin canalization and regeneration in Arabidopsis (grant \r\n2021/43/I/NZ1/01835) to E.M., and grant I 6123-B to J.F.","status":"public","issue":"12","publisher":"Elsevier","project":[{"grant_number":"I06123","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"quality_controlled":"1","ddc":["580"],"fulldoi":"https://doi.org/10.1016/j.tplants.2024.07.008","has_accepted_license":"1","language":[{"iso":"eng"}],"date_published":"2024-12-01T00:00:00Z","external_id":{"isi":["001375335200001"],"pmid":["39079770"]},"pmid":1,"author":[{"last_name":"Wójcikowska","first_name":"Barbara","full_name":"Wójcikowska, Barbara"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"}],"publication_identifier":{"issn":["1360-1385"]},"scopus_import":"1","supplementarymaterial":"no","corr_author":"1","page":"1279-1281","article_processing_charge":"No","type":"journal_article","day":"01"},{"fulldoi":"https://doi.org/10.1007/978-1-0716-3778-4_21","external_id":{"pmid":["38656499"]},"pmid":1,"date_published":"2024-04-25T00:00:00Z","alternative_title":["Methods in Molecular Biology"],"language":[{"iso":"eng"}],"page":"305-313","supplementarymaterial":"no","publication_identifier":{"isbn":["9781071637777"],"eissn":["1940-6029"]},"scopus_import":"1","author":[{"last_name":"Jayasree","first_name":"Aswathy","full_name":"Jayasree, Aswathy"},{"last_name":"Salava","first_name":"Hymavathi","full_name":"Salava, Hymavathi"},{"first_name":"Tomasz","full_name":"Nodzynski, Tomasz","last_name":"Nodzynski"},{"id":"055b7938-0b72-11ef-94eb-d14136011bb5","first_name":"Thula","full_name":"Sravankumar, Thula","last_name":"Sravankumar","orcid":"0000-0001-6925-6950"}],"day":"25","type":"book_chapter","article_processing_charge":"No","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-05-05T22:01:04Z","department":[{"_id":"JiFr"}],"editor":[{"full_name":"Maghuly, Fatemeh","first_name":"Fatemeh","last_name":"Maghuly"}],"_id":"15361","month":"04","oa_version":"None","publication":"Plant Functional Genomics","intvolume":"      2787","das_tickbox":"0","date_updated":"2026-10-15T08:09:43Z","citation":{"short":"A. Jayasree, H. Salava, T. Nodzynski, T. Sravankumar, in:, F. Maghuly (Ed.), Plant Functional Genomics, Springer Nature, 2024, pp. 305–313.","ieee":"A. Jayasree, H. Salava, T. Nodzynski, and T. Sravankumar, “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf,” in <i>Plant Functional Genomics</i>, vol. 2787, F. Maghuly, Ed. Springer Nature, 2024, pp. 305–313.","chicago":"Jayasree, Aswathy, Hymavathi Salava, Tomasz Nodzynski, and Thula Sravankumar. “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” In <i>Plant Functional Genomics</i>, edited by Fatemeh Maghuly, 2787:305–13. MIMB. Springer Nature, 2024. <a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">https://doi.org/10.1007/978-1-0716-3778-4_21</a>.","apa":"Jayasree, A., Salava, H., Nodzynski, T., &#38; Sravankumar, T. (2024). Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In F. Maghuly (Ed.), <i>Plant Functional Genomics</i> (Vol. 2787, pp. 305–313). Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">https://doi.org/10.1007/978-1-0716-3778-4_21</a>","ama":"Jayasree A, Salava H, Nodzynski T, Sravankumar T. Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Maghuly F, ed. <i>Plant Functional Genomics</i>. Vol 2787. MIMB. Springer Nature; 2024:305-313. doi:<a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">10.1007/978-1-0716-3778-4_21</a>","mla":"Jayasree, Aswathy, et al. “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” <i>Plant Functional Genomics</i>, edited by Fatemeh Maghuly, vol. 2787, Springer Nature, 2024, pp. 305–13, doi:<a href=\"https://doi.org/10.1007/978-1-0716-3778-4_21\">10.1007/978-1-0716-3778-4_21</a>.","ista":"Jayasree A, Salava H, Nodzynski T, Sravankumar T. 2024.Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf. In: Plant Functional Genomics. Methods in Molecular Biology, vol. 2787, 305–313."},"publication_status":"published","volume":2787,"researchdata_availability":"no","year":"2024","series_title":"MIMB","doi":"10.1007/978-1-0716-3778-4_21","quality_controlled":"1","status":"public","publisher":"Springer Nature","acknowledgement":"Special thanks to Dr. Marta Zwiewka for the support. Thanks to the Czech Science Foundation GA 20-20860Y for financial aid and support of A.S.S., respectively. Thanks go to Core Facility Cellular Imaging (CELLIM), and Plant Sciences Core Facility of CEITEC Masaryk University is acknowledged for the technical support.","title":"Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf","abstract":[{"lang":"eng","text":"Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented."}]},{"article_processing_charge":"No","type":"journal_article","day":"01","author":[{"last_name":"Hörmayer","orcid":"0000-0001-8295-2926","full_name":"Hörmayer, Lukas","first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"publication_identifier":{"eissn":["1748-7838"],"issn":["1001-0602"]},"scopus_import":"1","supplementarymaterial":"no","page":"761-762","corr_author":"1","language":[{"iso":"eng"}],"pmid":1,"external_id":{"isi":["001326684200001"],"pmid":["39354142"]},"date_published":"2024-11-01T00:00:00Z","fulldoi":"https://doi.org/10.1038/s41422-024-01035-x","abstract":[{"text":"Local wound signaling in plants informs the surrounding tissues about an injury and initiates the regeneration process. In a recent paper published in Cell, Yang and colleagues show the involvement of a single Pep family member from tomato in wound signaling and how exogenous application of this regeneration factor enhances transformation efficiency in crops.","lang":"eng"}],"title":"Feeling the danger: Local wound signaling in plants","publisher":"Springer Nature","status":"public","quality_controlled":"1","OA_type":"closed access","doi":"10.1038/s41422-024-01035-x","volume":34,"researchdata_availability":"no","year":"2024","publication_status":"published","citation":{"ista":"Hörmayer L, Friml J. 2024. Feeling the danger: Local wound signaling in plants. Cell Research. 34, 761–762.","ama":"Hörmayer L, Friml J. Feeling the danger: Local wound signaling in plants. <i>Cell Research</i>. 2024;34:761-762. doi:<a href=\"https://doi.org/10.1038/s41422-024-01035-x\">10.1038/s41422-024-01035-x</a>","mla":"Hörmayer, Lukas, and Jiří Friml. “Feeling the Danger: Local Wound Signaling in Plants.” <i>Cell Research</i>, vol. 34, Springer Nature, 2024, pp. 761–62, doi:<a href=\"https://doi.org/10.1038/s41422-024-01035-x\">10.1038/s41422-024-01035-x</a>.","chicago":"Hörmayer, Lukas, and Jiří Friml. “Feeling the Danger: Local Wound Signaling in Plants.” <i>Cell Research</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41422-024-01035-x\">https://doi.org/10.1038/s41422-024-01035-x</a>.","apa":"Hörmayer, L., &#38; Friml, J. (2024). Feeling the danger: Local wound signaling in plants. <i>Cell Research</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41422-024-01035-x\">https://doi.org/10.1038/s41422-024-01035-x</a>","short":"L. Hörmayer, J. Friml, Cell Research 34 (2024) 761–762.","ieee":"L. Hörmayer and J. Friml, “Feeling the danger: Local wound signaling in plants,” <i>Cell Research</i>, vol. 34. Springer Nature, pp. 761–762, 2024."},"date_updated":"2026-10-15T08:23:58Z","isi":1,"das_tickbox":"0","intvolume":"        34","publication":"Cell Research","oa_version":"None","month":"11","_id":"18311","article_type":"original","department":[{"_id":"JiFr"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-10-13T22:01:51Z"},{"language":[{"iso":"eng"}],"keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"pmid":1,"date_published":"2024-03-05T00:00:00Z","external_id":{"pmid":["38441122"]},"fulldoi":"https://doi.org/10.7554/elife.91523","ddc":["580"],"has_accepted_license":"1","article_processing_charge":"Yes","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"type":"journal_article","day":"05","author":[{"last_name":"Kulich","full_name":"Kulich, Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54","first_name":"Ivan"},{"first_name":"Julia","id":"07cf4637-baaf-11ee-9227-e1de57d1d69b","full_name":"Schmid, Julia","last_name":"Schmid"},{"last_name":"Teplova","id":"e3736151-106c-11ec-b916-c2558e2762c6","first_name":"Anastasiia","full_name":"Teplova, Anastasiia"},{"id":"44B04502-A9ED-11E9-B6FC-583AE6697425","first_name":"Linlin","full_name":"Qi, Linlin","last_name":"Qi","orcid":"0000-0001-5187-8401"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"scopus_import":"1","publication_identifier":{"issn":["2050-084X"]},"supplementarymaterial":"yes","article_number":"91523","corr_author":"1","oa":1,"date_updated":"2026-10-15T08:30:04Z","file":[{"relation":"main_file","file_size":11451904,"checksum":"a73a84d3bf97a6d09d24308ca6dd0a0c","date_created":"2024-04-03T13:18:00Z","content_type":"application/pdf","access_level":"open_access","success":1,"date_updated":"2024-04-03T13:18:00Z","file_id":"15288","file_name":"2024_eLife_Kulich.pdf","creator":"dernst"}],"das_tickbox":"1","related_material":{"record":[{"relation":"dissertation_contains","id":"22984","status":"public"}],"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/beneath-the-surface/","description":"News on ISTA website"}]},"intvolume":"        12","file_date_updated":"2024-04-03T13:18:00Z","oa_version":"Published Version","publication":"eLife","month":"03","dataavailabilitystatement":"All data generated in this study are included as a manuscript supporting files.","DOAJ_listed":"1","ec_funded":1,"_id":"15257","article_type":"original","department":[{"_id":"JiFr"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-04-02T11:35:58Z","abstract":[{"lang":"eng","text":"Root gravitropic bending represents a fundamental aspect of terrestrial plant physiology. Gravity is perceived by sedimentation of starch-rich plastids (statoliths) to the bottom of the central root cap cells. Following gravity perception, intercellular auxin transport is redirected downwards leading to an asymmetric auxin accumulation at the lower root side causing inhibition of cell expansion, ultimately resulting in downwards bending. How gravity-induced statoliths repositioning is translated into asymmetric auxin distribution remains unclear despite PIN auxin efflux carriers and the Negative Gravitropic Response of roots (NGR) proteins polarize along statolith sedimentation, thus providing a plausible mechanism for auxin flow redirection. In this study, using a functional NGR1-GFP construct, we visualized the NGR1 localization on the statolith surface and plasma membrane (PM) domains in close proximity to the statoliths, correlating with their movements. We determined that NGR1 binding to these PM domains is indispensable for NGR1 functionality and relies on cysteine acylation and adjacent polybasic regions as well as on lipid and sterol PM composition. Detailed timing of the early events following graviperception suggested that both NGR1 repolarization and initial auxin asymmetry precede the visible PIN3 polarization. This discrepancy motivated us to unveil a rapid, NGR-dependent translocation of PIN-activating AGCVIII kinase D6PK towards lower PMs of gravity-perceiving cells, thus providing an attractive model for rapid redirection of auxin fluxes following gravistimulation."}],"title":"Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism","acknowledgement":"The research leading to these results has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme grant agreement No 742985 and Austrian Science Fund (FWF): I3630-775 B25 to J.F. This research was also supported by the Lab Support Facility (LSF) and the Imaging and Optics Facility (IOF) of IST Austria, namely Tereza Bělinová for her help with the imaging. JS was supported by FemTECH fellowship.","publisher":"eLife Sciences Publications","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"status":"public","quality_controlled":"1","doi":"10.7554/elife.91523","researchdata_availability":"yes (supplementary data)","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","volume":12,"publication_status":"published","citation":{"ista":"Kulich I, Schmid J, Teplova A, Qi L, Friml J. 2024. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. eLife. 12, 91523.","mla":"Kulich, Ivan, et al. “Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6 Protein Kinase during Root Gravitropism.” <i>ELife</i>, vol. 12, 91523, eLife Sciences Publications, 2024, doi:<a href=\"https://doi.org/10.7554/elife.91523\">10.7554/elife.91523</a>.","ama":"Kulich I, Schmid J, Teplova A, Qi L, Friml J. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. <i>eLife</i>. 2024;12. doi:<a href=\"https://doi.org/10.7554/elife.91523\">10.7554/elife.91523</a>","apa":"Kulich, I., Schmid, J., Teplova, A., Qi, L., &#38; Friml, J. (2024). Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.91523\">https://doi.org/10.7554/elife.91523</a>","chicago":"Kulich, Ivan, Julia Schmid, Anastasiia Teplova, Linlin Qi, and Jiří Friml. “Rapid Translocation of NGR Proteins Driving Polarization of PIN-Activating D6 Protein Kinase during Root Gravitropism.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href=\"https://doi.org/10.7554/elife.91523\">https://doi.org/10.7554/elife.91523</a>.","ieee":"I. Kulich, J. Schmid, A. Teplova, L. Qi, and J. Friml, “Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism,” <i>eLife</i>, vol. 12. eLife Sciences Publications, 2024.","short":"I. Kulich, J. Schmid, A. Teplova, L. Qi, J. Friml, ELife 12 (2024)."}},{"language":[{"iso":"eng"}],"date_published":"2024-05-20T00:00:00Z","external_id":{"isi":["001301584600001"],"pmid":["38579717"]},"pmid":1,"ddc":["570"],"fulldoi":"https://doi.org/10.1016/j.devcel.2024.03.009","has_accepted_license":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_processing_charge":"Yes (via OA deal)","type":"journal_article","day":"20","author":[{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","first_name":"Lukas","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","last_name":"Hörmayer"},{"last_name":"Montesinos López","orcid":"0000-0001-9179-6099","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","full_name":"Montesinos López, Juan C"},{"last_name":"Trozzi","full_name":"Trozzi, N","first_name":"N"},{"last_name":"Spona","full_name":"Spona, Leonhard","id":"b52391fb-f636-11ee-939c-8a8c47552e8a","first_name":"Leonhard"},{"first_name":"Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","full_name":"Yoshida, Saiko","orcid":"0000-0001-6111-9353","last_name":"Yoshida"},{"last_name":"Marhavá","id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra","full_name":"Marhavá, Petra"},{"id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","first_name":"Silvia","full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","orcid":"0000-0002-5223-3346"},{"orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"},{"last_name":"Dagdas","full_name":"Dagdas, Y","first_name":"Y"},{"full_name":"Majda, M","first_name":"M","last_name":"Majda"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"scopus_import":"1","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"supplementarymaterial":"yes","corr_author":"1","page":"1333-1344.e4","isi":1,"date_updated":"2026-10-15T08:32:32Z","file":[{"relation":"main_file","checksum":"22b374fb50a40d380b7686c84258d271","file_size":5195262,"date_created":"2024-08-20T11:22:16Z","content_type":"application/pdf","access_level":"open_access","success":1,"date_updated":"2024-08-20T11:22:16Z","file_id":"17452","creator":"dernst","file_name":"2024_DevelopmentalCell_Hoermayer.pdf"}],"oa":1,"das_tickbox":"1","related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/"}]},"file_date_updated":"2024-08-20T11:22:16Z","intvolume":"        59","publication":"Developmental Cell","oa_version":"Published Version","dataavailabilitystatement":"All data reported in this paper will be shared by the lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","month":"05","_id":"15301","ec_funded":1,"department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"article_type":"original","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-04-08T12:07:57Z","abstract":[{"text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture.","lang":"eng"}],"title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","issue":"10","status":"public","publisher":"Elsevier","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988","name":"RNA-directed DNA methylation in plant development"}],"quality_controlled":"1","doi":"10.1016/j.devcel.2024.03.009","researchdata_availability":"upon request","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"volume":59,"year":"2024","citation":{"mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>."},"publication_status":"published"},{"intvolume":"        17","file_date_updated":"2024-12-03T11:08:09Z","publication":"Molecular Plant","oa_version":"Published Version","oa":1,"isi":1,"file":[{"access_level":"open_access","date_created":"2024-12-03T11:08:09Z","content_type":"application/pdf","file_size":3308945,"checksum":"a11feea4b1677df76b632eca04bfc1dd","relation":"main_file","file_name":"2024_MolecularPlant_Kralova.pdf","creator":"dernst","success":1,"file_id":"18612","date_updated":"2024-12-03T11:08:09Z"}],"date_updated":"2026-10-15T08:30:20Z","das_tickbox":"0","article_type":"original","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-11-28T11:13:35Z","month":"12","_id":"18596","publisher":"Elsevier","issue":"12","status":"public","quality_controlled":"1","abstract":[{"lang":"eng","text":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis."}],"title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis","acknowledgement":"We dedicate this paper to the deceased Petr Galuszka for his inspiration and support of our project. We thank Prof. Peter Hedden for constructive criticism of the manuscript and English editing. No conflict of interest is declared.","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"year":"2024","researchdata_availability":"no","volume":17,"OA_place":"publisher","publication_status":"published","citation":{"mla":"Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>, vol. 17, no. 12, Elsevier, 2024, pp. 1850–65, doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>.","ama":"Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. 2024;17(12):1850-1865. doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>","ista":"Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.","ieee":"M. Králová <i>et al.</i>, “A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis,” <i>Molecular Plant</i>, vol. 17, no. 12. Elsevier, pp. 1850–1865, 2024.","short":"M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C. Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml, O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.","apa":"Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., … Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>","chicago":"Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>."},"OA_type":"hybrid","doi":"10.1016/j.molp.2024.11.001","external_id":{"isi":["001373778300001"],"pmid":["39501563"]},"pmid":1,"date_published":"2024-12-02T00:00:00Z","language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1016/j.molp.2024.11.001","ddc":["580"],"has_accepted_license":"1","day":"02","article_processing_charge":"Yes (in subscription journal)","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"type":"journal_article","supplementarymaterial":"yes","page":"1850-1865","author":[{"last_name":"Králová","full_name":"Králová, Michaela","first_name":"Michaela"},{"last_name":"Kubalová","first_name":"Ivona","full_name":"Kubalová, Ivona"},{"last_name":"Hajný","first_name":"Jakub","full_name":"Hajný, Jakub"},{"id":"946011F4-3E71-11EA-860B-C7A73DDC885E","first_name":"Karolina","full_name":"Kubiasova, Karolina","last_name":"Kubiasova","orcid":"0000-0001-5630-9419"},{"last_name":"Vagaská","first_name":"Karolína","full_name":"Vagaská, Karolína"},{"id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","first_name":"Zengxiang","full_name":"Ge, Zengxiang","orcid":"0000-0001-9381-3577","last_name":"Ge"},{"orcid":"0000-0003-1286-7368","last_name":"Gallei","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C"},{"full_name":"Semerádová, Hana","id":"42FE702E-F248-11E8-B48F-1D18A9856A87","first_name":"Hana","last_name":"Semerádová"},{"first_name":"Anna","full_name":"Kuchařová, Anna","last_name":"Kuchařová"},{"last_name":"Hönig","full_name":"Hönig, Martin","first_name":"Martin"},{"last_name":"Monzer","first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline"},{"last_name":"Kovačik","first_name":"Martin","full_name":"Kovačik, Martin"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Novák","full_name":"Novák, Ondřej","first_name":"Ondřej"},{"last_name":"Benková","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"},{"last_name":"Ikeda","full_name":"Ikeda, Yoshihisa","first_name":"Yoshihisa"},{"last_name":"Zalabák","first_name":"David","full_name":"Zalabák, David"}],"publication_identifier":{"issn":["1674-2052"]},"scopus_import":"1"},{"has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.neuron.2024.03.019","ddc":["570"],"pmid":1,"date_published":"2024-06-19T00:00:00Z","external_id":{"pmid":["38636524"],"isi":["001300571400001"]},"language":[{"iso":"eng"}],"corr_author":"1","page":"2045-2061.e10","supplementarymaterial":"yes","scopus_import":"1","publication_identifier":{"eissn":["1097-4199"],"issn":["0896-6273"]},"author":[{"orcid":"0000-0002-8602-4374","last_name":"Rangel Guerrero","full_name":"Rangel Guerrero, Dámaris K","id":"4871BCE6-F248-11E8-B48F-1D18A9856A87","first_name":"Dámaris K"},{"full_name":"Balueva, Kira","first_name":"Kira","last_name":"Balueva"},{"last_name":"Barayeu","full_name":"Barayeu, Uladzislau","id":"b515be12-ec90-11ea-b966-d0b5e15613d2","first_name":"Uladzislau"},{"last_name":"Baracskay","first_name":"Peter","id":"361CC00E-F248-11E8-B48F-1D18A9856A87","full_name":"Baracskay, Peter"},{"orcid":"0000-0002-1807-1929","last_name":"Gridchyn","id":"4B60654C-F248-11E8-B48F-1D18A9856A87","first_name":"Igor","full_name":"Gridchyn, Igor"},{"full_name":"Nardin, Michele","id":"30BD0376-F248-11E8-B48F-1D18A9856A87","first_name":"Michele","last_name":"Nardin","orcid":"0000-0001-8849-6570"},{"full_name":"Roth, Chiara N","id":"37BB4FB6-F248-11E8-B48F-1D18A9856A87","first_name":"Chiara N","last_name":"Roth"},{"full_name":"Wulff, Peer","first_name":"Peer","last_name":"Wulff"},{"last_name":"Csicsvari","orcid":"0000-0002-5193-4036","full_name":"Csicsvari, Jozsef L","id":"3FA14672-F248-11E8-B48F-1D18A9856A87","first_name":"Jozsef L"}],"day":"19","type":"journal_article","article_processing_charge":"Yes (via OA deal)","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-05-12T22:01:03Z","article_type":"original","department":[{"_id":"JoCs"}],"_id":"15381","month":"06","dataavailabilitystatement":"Data reported in this paper can be shared by the lead contact upon justified request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this work is available from the corresponding authors upon request.","publication":"Neuron","oa_version":"Published Version","intvolume":"       112","file_date_updated":"2025-01-09T09:15:31Z","das_tickbox":"1","oa":1,"date_updated":"2026-10-15T08:38:29Z","isi":1,"file":[{"access_level":"open_access","date_created":"2025-01-09T09:15:31Z","content_type":"application/pdf","file_size":9149079,"checksum":"de5b18ff293d42bd90e83a193e889844","relation":"main_file","file_name":"2024_Neuron_RangelGuerrero.pdf","creator":"dernst","success":1,"date_updated":"2025-01-09T09:15:31Z","file_id":"18798"}],"publication_status":"published","OA_place":"publisher","citation":{"short":"D.K. Rangel Guerrero, K. Balueva, U. Barayeu, P. Baracskay, I. Gridchyn, M. Nardin, C.N. Roth, P. Wulff, J.L. Csicsvari, Neuron 112 (2024) 2045–2061.e10.","ieee":"D. K. Rangel Guerrero <i>et al.</i>, “Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning,” <i>Neuron</i>, vol. 112, no. 12. Cell Press, p. 2045–2061.e10, 2024.","chicago":"Rangel Guerrero, Dámaris K, Kira Balueva, Uladzislau Barayeu, Peter Baracskay, Igor Gridchyn, Michele Nardin, Chiara N Roth, Peer Wulff, and Jozsef L Csicsvari. “Hippocampal Cholecystokinin-Expressing Interneurons Regulate Temporal Coding and Contextual Learning.” <i>Neuron</i>. Cell Press, 2024. <a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">https://doi.org/10.1016/j.neuron.2024.03.019</a>.","apa":"Rangel Guerrero, D. K., Balueva, K., Barayeu, U., Baracskay, P., Gridchyn, I., Nardin, M., … Csicsvari, J. L. (2024). Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. <i>Neuron</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">https://doi.org/10.1016/j.neuron.2024.03.019</a>","ama":"Rangel Guerrero DK, Balueva K, Barayeu U, et al. Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. <i>Neuron</i>. 2024;112(12):2045-2061.e10. doi:<a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">10.1016/j.neuron.2024.03.019</a>","mla":"Rangel Guerrero, Dámaris K., et al. “Hippocampal Cholecystokinin-Expressing Interneurons Regulate Temporal Coding and Contextual Learning.” <i>Neuron</i>, vol. 112, no. 12, Cell Press, 2024, p. 2045–2061.e10, doi:<a href=\"https://doi.org/10.1016/j.neuron.2024.03.019\">10.1016/j.neuron.2024.03.019</a>.","ista":"Rangel Guerrero DK, Balueva K, Barayeu U, Baracskay P, Gridchyn I, Nardin M, Roth CN, Wulff P, Csicsvari JL. 2024. Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning. Neuron. 112(12), 2045–2061.e10."},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"researchdata_availability":"upon request","volume":112,"year":"2024","doi":"10.1016/j.neuron.2024.03.019","OA_type":"hybrid","quality_controlled":"1","publisher":"Cell Press","project":[{"call_identifier":"FWF","_id":"2654F984-B435-11E9-9278-68D0E5697425","grant_number":"I 3713-B27","name":"Interneuro plasticity during spatial learning"}],"issue":"12","status":"public","acknowledgement":"We thank the kind donations from Andrea Varro, Brian Sauer, Edward Boyden, and Peter Jonas. We thank Jago Wallenschus, Kerstin Kronenbitter, and Didier Gremelle for outstanding technical support; Laura Bollepalli for initial viral targeting experiments; Cihan Önal for initial electrophysiology experiments; Yoav Ben-Simon for histological advice; and Anton Nikitenko for contributing to the analysis. We acknowledge support from the Miba Machine Shop, Bioimaging-, Life Science- and Pre-Clinical Facilities at ISTA. This work was supported by the Austrian Science Fund (FWF I3713 to J.C. as part of the FOR 2143 research consortium), the Deutsche Forschungsgemeinschaft (DFG) (WU 503/2-2 to P.W.), and the Medical Research Council, United Kingdom (grant G1100546/2 to P.W.).","title":"Hippocampal cholecystokinin-expressing interneurons regulate temporal coding and contextual learning","abstract":[{"text":"Cholecystokinin-expressing interneurons (CCKIs) are hypothesized to shape pyramidal cell-firing patterns and regulate network oscillations and related network state transitions. To directly probe their role in the CA1 region, we silenced their activity using optogenetic and chemogenetic tools in mice. Opto-tagged CCKIs revealed a heterogeneous population, and their optogenetic silencing triggered wide disinhibitory network changes affecting both pyramidal cells and other interneurons. CCKI silencing enhanced pyramidal cell burst firing and altered the temporal coding of place cells: theta phase precession was disrupted, whereas sequence reactivation was enhanced. Chemogenetic CCKI silencing did not alter the acquisition of spatial reference memories on the Morris water maze but enhanced the recall of contextual fear memories and enabled selective recall when similar environments were tested. This work suggests the key involvement of CCKIs in the control of place-cell temporal coding and the formation of contextual memories.","lang":"eng"}]},{"day":"16","article_processing_charge":"Yes","type":"journal_article","supplementarymaterial":"yes","corr_author":"1","article_number":"031030","author":[{"orcid":"0000-0002-1965-4309","last_name":"Hawaldar","full_name":"Hawaldar, Samarth","id":"221708e1-1ff6-11ee-9fa6-85146607433e","first_name":"Samarth"},{"first_name":"Prakriti","full_name":"Shahi, Prakriti","last_name":"Shahi"},{"first_name":"Allison L.","full_name":"Carter, Allison L.","last_name":"Carter"},{"last_name":"Rey","first_name":"Ana Maria","full_name":"Rey, Ana Maria"},{"last_name":"Bollinger","first_name":"John J.","full_name":"Bollinger, John J."},{"last_name":"Shankar","first_name":"Athreya","full_name":"Shankar, Athreya"}],"scopus_import":"1","publication_identifier":{"eissn":["2160-3308"]},"arxiv":1,"date_published":"2024-08-16T00:00:00Z","external_id":{"arxiv":["2312.10681"],"isi":["001293977800002"]},"language":[{"iso":"eng"}],"ddc":["530"],"fulldoi":"https://doi.org/10.1103/PhysRevX.14.031030","has_accepted_license":"1","status":"public","issue":"3","publisher":"American Physical Society","quality_controlled":"1","title":"Bilayer crystals of trapped ions for quantum information processing","abstract":[{"text":"Trapped-ion systems are a leading platform for quantum information processing, but they are currently limited to 1D and 2D arrays, which imposes restrictions on both their scalability and their range of applications. Here, we propose a path to overcome this limitation by demonstrating that Penning traps can be used to realize remarkably clean bilayer crystals, wherein hundreds of ions self-organize into two well-defined layers. These bilayer crystals are made possible by the inclusion of an anharmonic trapping potential, which is readily implementable with current technology. We study the normal modes of this system and discover salient differences compared to the modes of single-plane crystals. The bilayer geometry and the unique properties of the normal modes open new opportunities—in particular, in quantum sensing and quantum simulation—that are not straightforward in single-plane crystals. Furthermore, we illustrate that it may be possible to extend the ideas presented here to realize multilayer crystals with more than two layers. Our work increases the dimensionality of trapped-ion systems by efficiently utilizing all three spatial dimensions, and it lays the foundation for a new generation of quantum information processing experiments with multilayer 3D crystals of trapped ions.","lang":"eng"}],"acknowledgement":"We thank M. Miskeen Khan, Jennifer Lilieholm, and Wes Johnson for a careful reading and feedback on the manuscript. We acknowledge discussions with Dan Dubin, John Zaris, and Scott Parker. S. H. acknowledges the support of Kishore Vaigyanik Protsahan Yojana, Department of Science and Technology, Government of India. A. S. acknowledges the support of a C. V. Raman post-doctoral fellowship. A. L. C., A. M. R., and J. J. B. acknowledge funding from the U.S. Department of Energy, Office of Science, NQI Science Research Centers, Quantum Systems Accelerator (QSA), a collaboration between the U.S. Department of Energy, Office of Science and other agencies. A. M. R. acknowledges additional support from VBFF, ARO Grant No. W911NF-24-1-0128, by the NSF Grants No. JILA-PFC PHY-2317149 and No. QLCI-OMA-2016244, and by NIST. J. J. B. acknowledges additional support from the DARPA ONISQ program and AFOSR Grant No. FA9550-201-0019.","volume":14,"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"researchdata_availability":"no","year":"2024","citation":{"ista":"Hawaldar S, Shahi P, Carter AL, Rey AM, Bollinger JJ, Shankar A. 2024. Bilayer crystals of trapped ions for quantum information processing. Physical Review X. 14(3), 031030.","ama":"Hawaldar S, Shahi P, Carter AL, Rey AM, Bollinger JJ, Shankar A. Bilayer crystals of trapped ions for quantum information processing. <i>Physical Review X</i>. 2024;14(3). doi:<a href=\"https://doi.org/10.1103/PhysRevX.14.031030\">10.1103/PhysRevX.14.031030</a>","mla":"Hawaldar, Samarth, et al. “Bilayer Crystals of Trapped Ions for Quantum Information Processing.” <i>Physical Review X</i>, vol. 14, no. 3, 031030, American Physical Society, 2024, doi:<a href=\"https://doi.org/10.1103/PhysRevX.14.031030\">10.1103/PhysRevX.14.031030</a>.","chicago":"Hawaldar, Samarth, Prakriti Shahi, Allison L. Carter, Ana Maria Rey, John J. Bollinger, and Athreya Shankar. “Bilayer Crystals of Trapped Ions for Quantum Information Processing.” <i>Physical Review X</i>. American Physical Society, 2024. <a href=\"https://doi.org/10.1103/PhysRevX.14.031030\">https://doi.org/10.1103/PhysRevX.14.031030</a>.","apa":"Hawaldar, S., Shahi, P., Carter, A. L., Rey, A. M., Bollinger, J. J., &#38; Shankar, A. (2024). Bilayer crystals of trapped ions for quantum information processing. <i>Physical Review X</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevX.14.031030\">https://doi.org/10.1103/PhysRevX.14.031030</a>","short":"S. Hawaldar, P. Shahi, A.L. Carter, A.M. Rey, J.J. Bollinger, A. Shankar, Physical Review X 14 (2024).","ieee":"S. Hawaldar, P. Shahi, A. L. Carter, A. M. Rey, J. J. Bollinger, and A. Shankar, “Bilayer crystals of trapped ions for quantum information processing,” <i>Physical Review X</i>, vol. 14, no. 3. American Physical Society, 2024."},"publication_status":"published","doi":"10.1103/PhysRevX.14.031030","file_date_updated":"2024-09-06T09:43:53Z","intvolume":"        14","oa_version":"Published Version","publication":"Physical Review X","isi":1,"date_updated":"2026-10-15T09:06:46Z","file":[{"relation":"main_file","file_size":3909653,"checksum":"5d39b7dda67fd7b9a960235f6f38e280","date_created":"2024-09-06T09:43:53Z","content_type":"application/pdf","access_level":"open_access","success":1,"date_updated":"2024-09-06T09:43:53Z","file_id":"17757","creator":"cchlebak","file_name":"2024_PhysRevX_Hawaldar.pdf"}],"oa":1,"das_tickbox":"0","department":[{"_id":"JoFi"}],"article_type":"original","date_created":"2024-09-01T22:01:08Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"08","_id":"17477","DOAJ_listed":"1"},{"scopus_import":"1","publication_identifier":{"eissn":["2211-1247"]},"author":[{"first_name":"Shih Pi","full_name":"Ku, Shih Pi","last_name":"Ku"},{"full_name":"Atucha, Erika","first_name":"Erika","last_name":"Atucha"},{"last_name":"Alavi","first_name":"Nico","full_name":"Alavi, Nico"},{"first_name":"Halla","full_name":"Mulla-Osman, Halla","last_name":"Mulla-Osman"},{"last_name":"Kayumova","first_name":"Rukhshona","full_name":"Kayumova, Rukhshona"},{"first_name":"Motoharu","full_name":"Yoshida, Motoharu","last_name":"Yoshida"},{"first_name":"Jozsef L","id":"3FA14672-F248-11E8-B48F-1D18A9856A87","full_name":"Csicsvari, Jozsef L","orcid":"0000-0002-5193-4036","last_name":"Csicsvari"},{"first_name":"Magdalena M.","full_name":"Sauvage, Magdalena M.","last_name":"Sauvage"}],"article_number":"114276","supplementarymaterial":"yes","type":"journal_article","article_processing_charge":"Yes (in subscription journal)","day":"25","has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.celrep.2024.114276","ddc":["570"],"language":[{"iso":"eng"}],"external_id":{"isi":["001252792600001"]},"date_published":"2024-06-25T00:00:00Z","doi":"10.1016/j.celrep.2024.114276","citation":{"apa":"Ku, S. P., Atucha, E., Alavi, N., Mulla-Osman, H., Kayumova, R., Yoshida, M., … Sauvage, M. M. (2024). Phase locking of hippocampal CA3 neurons to distal CA1 theta oscillations selectively predicts memory performance. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2024.114276\">https://doi.org/10.1016/j.celrep.2024.114276</a>","chicago":"Ku, Shih Pi, Erika Atucha, Nico Alavi, Halla Mulla-Osman, Rukhshona Kayumova, Motoharu Yoshida, Jozsef L Csicsvari, and Magdalena M. Sauvage. “Phase Locking of Hippocampal CA3 Neurons to Distal CA1 Theta Oscillations Selectively Predicts Memory Performance.” <i>Cell Reports</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.celrep.2024.114276\">https://doi.org/10.1016/j.celrep.2024.114276</a>.","ieee":"S. P. Ku <i>et al.</i>, “Phase locking of hippocampal CA3 neurons to distal CA1 theta oscillations selectively predicts memory performance,” <i>Cell Reports</i>, vol. 43, no. 6. Elsevier, 2024.","short":"S.P. Ku, E. Atucha, N. Alavi, H. Mulla-Osman, R. Kayumova, M. Yoshida, J.L. Csicsvari, M.M. Sauvage, Cell Reports 43 (2024).","ista":"Ku SP, Atucha E, Alavi N, Mulla-Osman H, Kayumova R, Yoshida M, Csicsvari JL, Sauvage MM. 2024. Phase locking of hippocampal CA3 neurons to distal CA1 theta oscillations selectively predicts memory performance. Cell Reports. 43(6), 114276.","mla":"Ku, Shih Pi, et al. “Phase Locking of Hippocampal CA3 Neurons to Distal CA1 Theta Oscillations Selectively Predicts Memory Performance.” <i>Cell Reports</i>, vol. 43, no. 6, 114276, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.celrep.2024.114276\">10.1016/j.celrep.2024.114276</a>.","ama":"Ku SP, Atucha E, Alavi N, et al. Phase locking of hippocampal CA3 neurons to distal CA1 theta oscillations selectively predicts memory performance. <i>Cell Reports</i>. 2024;43(6). doi:<a href=\"https://doi.org/10.1016/j.celrep.2024.114276\">10.1016/j.celrep.2024.114276</a>"},"publication_status":"published","researchdata_availability":"upon request","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","volume":43,"acknowledgement":"We would like to thank J. Maiwald for her assistance in animal behavior training, experiments, and brain slice preparation; D. Koch for her assistance in recording drive building and brain slicing; K. Kaefer and J. Wallenschus (IST Austria) for their initial technical support; S. Mikulovich for her comments on an early version of the manuscript; C. Reichert for his comments on SVM analyses; and J. Pakan for English proofreading. This project is funded by the DFG (CRC 779 and CRC 1436).","abstract":[{"lang":"eng","text":"How the coordination of neuronal spiking and brain rhythms between hippocampal subregions supports memory function remains elusive. We studied the interregional coordination of CA3 neuronal spiking with CA1 theta oscillations by recording electrophysiological signals along the proximodistal axis of the hippocampus in rats that were performing a high-memory-demand recognition memory task adapted from humans. We found that CA3 population spiking occurs preferentially at the peak of distal CA1 theta oscillations when memory was tested but only when previously encountered stimuli were presented. In addition, decoding analyses revealed that only population cell firing of proximal CA3 together with that of distal CA1 can predict performance at test in the present non-spatial task. Overall, our work demonstrates an important role for the synchronization of CA3 neuronal activity with CA1 theta oscillations during memory testing."}],"title":"Phase locking of hippocampal CA3 neurons to distal CA1 theta oscillations selectively predicts memory performance","quality_controlled":"1","publisher":"Elsevier","status":"public","issue":"6","_id":"17089","month":"06","dataavailabilitystatement":"Data published in this manuscript are available upon request.\r\n\r\nAll the original code has been deposited at Zenodo: https://zenodo.org/records/11125633. The DOI is listed in the key resources table.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available upon request.","date_created":"2024-06-02T22:00:56Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","department":[{"_id":"JoCs"}],"das_tickbox":"1","oa":1,"isi":1,"date_updated":"2026-10-15T08:37:28Z","file":[{"relation":"main_file","checksum":"9b43f8ca5e5a12ae96e3fb9df06385c1","file_size":4371015,"date_created":"2024-06-03T07:12:45Z","content_type":"application/pdf","access_level":"open_access","success":1,"file_id":"17096","date_updated":"2024-06-03T07:12:45Z","file_name":"2024_CellReports_Ku.pdf","creator":"dernst"}],"publication":"Cell Reports","oa_version":"Published Version","intvolume":"        43","file_date_updated":"2024-06-03T07:12:45Z"},{"quality_controlled":"1","status":"public","publisher":"American Physical Society","acknowledgement":"We gratefully acknowledge valuable discussions with Uros Delic, Lorenzo Magrini, and Corentin Gut. This work was supported by the European Union’s Horizon 2020 research and innovation program under Grant No. 863132 (iQLev) and No. 101080143 (SuperMeQ), the European Research Council under Grant No. 951234 (ERC Synergy QXtreme), the Austrian and Bavarian Academy of Sciences (Topical Team SGQ), the Alexander von Humboldt Foundation through a Feodor Lynen Fellowship (P.S.), the Swedish Research Council under Grant No. 2020-00381 (G.H.), and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) via Germany’s Excellence Strategy EXC-2111-390814868 (H.H., R.G.).","title":"Remote sensing of a levitated superconductor with a flux-tunable microwave cavity","abstract":[{"text":"We present a cavity-electromechanical system comprising a superconducting quantum interference device which is embedded in a microwave resonator and coupled via a pickup loop to a 6-μ⁢g magnetically levitated superconducting sphere. The motion of the sphere in the magnetic trap induces a frequency shift in the SQUID-cavity system. We use microwave spectroscopy to characterize the system, and we demonstrate that the electromechanical interaction is tunable. The measured displacement sensitivity of 10−7m/√Hz defines a path towards ground-state cooling of levitated particles with Planck-scale masses at millikelvin environment temperatures.","lang":"eng"}],"citation":{"apa":"Schmidt, P., Claessen, R., Higgins, G., Hofer, J., Hansen, J. J., Asenbaum, P., … Aspelmeyer, M. (2024). Remote sensing of a levitated superconductor with a flux-tunable microwave cavity. <i>Physical Review Applied</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevApplied.22.014078\">https://doi.org/10.1103/PhysRevApplied.22.014078</a>","chicago":"Schmidt, Philip, Remi Claessen, Gerard Higgins, Joachim Hofer, Jannek J. Hansen, Peter Asenbaum, Martin Zemlicka, et al. “Remote Sensing of a Levitated Superconductor with a Flux-Tunable Microwave Cavity.” <i>Physical Review Applied</i>. American Physical Society, 2024. <a href=\"https://doi.org/10.1103/PhysRevApplied.22.014078\">https://doi.org/10.1103/PhysRevApplied.22.014078</a>.","ieee":"P. Schmidt <i>et al.</i>, “Remote sensing of a levitated superconductor with a flux-tunable microwave cavity,” <i>Physical Review Applied</i>, vol. 22. American Physical Society, 2024.","short":"P. Schmidt, R. Claessen, G. Higgins, J. Hofer, J.J. Hansen, P. Asenbaum, M. Zemlicka, K. Uhl, R. Kleiner, R. Gross, H. Huebl, M. Trupke, M. Aspelmeyer, Physical Review Applied 22 (2024).","ista":"Schmidt P, Claessen R, Higgins G, Hofer J, Hansen JJ, Asenbaum P, Zemlicka M, Uhl K, Kleiner R, Gross R, Huebl H, Trupke M, Aspelmeyer M. 2024. Remote sensing of a levitated superconductor with a flux-tunable microwave cavity. Physical Review Applied. 22, 014078.","mla":"Schmidt, Philip, et al. “Remote Sensing of a Levitated Superconductor with a Flux-Tunable Microwave Cavity.” <i>Physical Review Applied</i>, vol. 22, 014078, American Physical Society, 2024, doi:<a href=\"https://doi.org/10.1103/PhysRevApplied.22.014078\">10.1103/PhysRevApplied.22.014078</a>.","ama":"Schmidt P, Claessen R, Higgins G, et al. Remote sensing of a levitated superconductor with a flux-tunable microwave cavity. <i>Physical Review Applied</i>. 2024;22. doi:<a href=\"https://doi.org/10.1103/PhysRevApplied.22.014078\">10.1103/PhysRevApplied.22.014078</a>"},"publication_status":"published","researchdata_availability":"yes","volume":22,"year":"2024","doi":"10.1103/PhysRevApplied.22.014078","oa_version":"Preprint","publication":"Physical Review Applied","intvolume":"        22","das_tickbox":"0","isi":1,"date_updated":"2026-10-15T09:06:26Z","oa":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-08-11T22:01:12Z","department":[{"_id":"JoFi"}],"article_type":"original","_id":"17410","month":"07","day":"30","type":"journal_article","article_processing_charge":"No","article_number":"014078","supplementarymaterial":"yes","main_file_link":[{"url":"https://doi.org/10.48550/arXiv.2401.08854","open_access":"1"}],"publication_identifier":{"eissn":["2331-7019"]},"scopus_import":"1","author":[{"full_name":"Schmidt, Philip","first_name":"Philip","last_name":"Schmidt"},{"last_name":"Claessen","first_name":"Remi","full_name":"Claessen, Remi"},{"last_name":"Higgins","first_name":"Gerard","full_name":"Higgins, Gerard"},{"last_name":"Hofer","first_name":"Joachim","full_name":"Hofer, Joachim"},{"first_name":"Jannek J.","full_name":"Hansen, Jannek J.","last_name":"Hansen"},{"last_name":"Asenbaum","first_name":"Peter","full_name":"Asenbaum, Peter"},{"full_name":"Zemlicka, Martin","id":"2DCF8DE6-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Zemlicka","orcid":"0009-0005-0878-3032"},{"first_name":"Kevin","full_name":"Uhl, Kevin","last_name":"Uhl"},{"full_name":"Kleiner, Reinhold","first_name":"Reinhold","last_name":"Kleiner"},{"full_name":"Gross, Rudolf","first_name":"Rudolf","last_name":"Gross"},{"first_name":"Hans","full_name":"Huebl, Hans","last_name":"Huebl"},{"first_name":"Michael","full_name":"Trupke, Michael","last_name":"Trupke"},{"full_name":"Aspelmeyer, Markus","first_name":"Markus","last_name":"Aspelmeyer"}],"external_id":{"isi":["001284571700002"],"arxiv":["2401.08854"]},"date_published":"2024-07-30T00:00:00Z","arxiv":1,"language":[{"iso":"eng"}],"fulldoi":"https://doi.org/10.1103/PhysRevApplied.22.014078"},{"isi":1,"file":[{"file_id":"15184","date_updated":"2024-03-25T09:31:58Z","success":1,"creator":"dernst","file_name":"2024_AstrophysicalJourn_Matthee.pdf","relation":"main_file","file_size":6047536,"checksum":"dc7af4694f9f94a551417ab49fa43edf","content_type":"application/pdf","date_created":"2024-03-25T09:31:58Z","access_level":"open_access"}],"date_updated":"2026-10-15T09:15:55Z","oa":1,"related_material":{"link":[{"url":"https://ista.ac.at/en/news/baby-quasars-growing-supermassive-black-holes/","relation":"press_release","description":"News on ISTA website"}]},"das_tickbox":"0","file_date_updated":"2024-03-25T09:31:58Z","intvolume":"       963","publication":"The Astrophysical Journal","oa_version":"Published Version","month":"03","_id":"15180","department":[{"_id":"JoMa"}],"APC_amount":"5666,27 EUR","article_type":"original","date_created":"2024-03-25T08:54:47Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","abstract":[{"lang":"eng","text":"Characterizing the prevalence and properties of faint active galactic nuclei (AGNs) in the early Universe is key for understanding the formation of supermassive black holes (SMBHs) and determining their role in cosmic reionization. We perform a spectroscopic search for broad Hα emitters at z ≈ 4–6 using deep JWST/NIRCam imaging and wide field slitless spectroscopy from the EIGER and FRESCO surveys. We identify 20 Hα lines at z = 4.2–5.5 that have broad components with line widths from ∼1200–3700 km s−1, contributing ∼30%–90% of the total line flux. We interpret these broad components as being powered by accretion onto SMBHs with implied masses ∼107–8M⊙. In the UV luminosity range MUV,AGN+host = −21 to −18, we measure number densities of ≈10−5 cMpc−3. This is an order of magnitude higher than expected from extrapolating quasar UV luminosity functions (LFs). Yet, such AGN are found in only <1% of star-forming galaxies at z ∼ 5. The number density discrepancy is much lower when compared to the broad Hα LF. The SMBH mass function agrees with large cosmological simulations. In two objects, we detect complex Hα profiles that we tentatively interpret as caused by absorption signatures from dense gas fueling SMBH growth and outflows. We may be witnessing early AGN feedback that will clear dust-free pathways through which more massive blue quasars are seen. We uncover a strong correlation between reddening and the fraction of total galaxy luminosity arising from faint AGN. This implies that early SMBH growth is highly obscured and that faint AGN are only minor contributors to cosmic reionization."}],"title":"Little Red Dots: An abundant population of faint active galactic nuclei at z ∼ 5 revealed by the EIGER and FRESCO JWST surveys","acknowledgement":"We thank the anonymous referee for constructive comments that helped improve the manuscript. This work is based on observations made with the NASA/ESA/CSA James Webb Space Telescope. The data were obtained from the Mikulski Archive for Space Telescopes at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-03127 for JWST. These observations are associated with program Nos. 1243 and 1895. The specific observations analyzed can be accessed via doi:10.17909/4xx0-zj76. Funded by the European Union (ERC, AGENTS, 101076224). Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council. Neither the European Union nor the granting authority can be held responsible for them. R.P.N. acknowledges funding from JWST programs GO-1933 and GO-2279. Support for this work for R.P.N. was provided by NASA through the NASA Hubble Fellowship grant HST-HF2-51515.001-A awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Incorporated, under NASA contract NAS5-26555. Support for this work for G.I. was provided by NASA through grant JWST-GO-01895 awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555. This work has received funding from the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract No. MB22.00072, as well as from the Swiss National Science Foundation (SNSF) through project grant 200020_207349. The Cosmic Dawn Center (DAWN) is funded by the Danish National Research Foundation under grant No. 140.\r\nFacility: JWST - James Webb Space Telescope, HST - Hubble Space Telescope satellite\r\nSoftware:​​​​​​​ Python, matplotlib (Hunter 2007), numpy (Harris et al. 2020), scipy (Virtanen et al. 2020), Astropy (Astropy Collaboration et al. 2013, 2018), Imfit (Erwin 2015).","status":"public","issue":"2","publisher":"IOP Publishing","project":[{"name":"Young galaxies as tracers and agents of cosmic reionization","_id":"bd9b2118-d553-11ed-ba76-db24564edfea","grant_number":"101076224"}],"quality_controlled":"1","OA_type":"gold","doi":"10.3847/1538-4357/ad2345","volume":963,"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","researchdata_availability":"no","OA_place":"publisher","publication_status":"published","citation":{"ama":"Matthee JJ, Naidu RP, Brammer G, et al. Little Red Dots: An abundant population of faint active galactic nuclei at z ∼ 5 revealed by the EIGER and FRESCO JWST surveys. <i>The Astrophysical Journal</i>. 2024;963(2). doi:<a href=\"https://doi.org/10.3847/1538-4357/ad2345\">10.3847/1538-4357/ad2345</a>","mla":"Matthee, Jorryt J., et al. “Little Red Dots: An Abundant Population of Faint Active Galactic Nuclei at z ∼ 5 Revealed by the EIGER and FRESCO JWST Surveys.” <i>The Astrophysical Journal</i>, vol. 963, no. 2, 129, IOP Publishing, 2024, doi:<a href=\"https://doi.org/10.3847/1538-4357/ad2345\">10.3847/1538-4357/ad2345</a>.","ista":"Matthee JJ, Naidu RP, Brammer G, Chisholm J, Eilers A-C, Goulding A, Greene J, Kashino D, Labbe I, Lilly SJ, Mackenzie R, Oesch PA, Weibel A, Wuyts S, Xiao M, Bordoloi R, Bouwens R, van Dokkum P, Illingworth G, Kramarenko I, Maseda MV, Mason C, Meyer RA, Nelson EJ, Reddy NA, Shivaei I, Simcoe RA, Yue M. 2024. Little Red Dots: An abundant population of faint active galactic nuclei at z ∼ 5 revealed by the EIGER and FRESCO JWST surveys. The Astrophysical Journal. 963(2), 129.","short":"J.J. Matthee, R.P. Naidu, G. Brammer, J. Chisholm, A.-C. Eilers, A. Goulding, J. Greene, D. Kashino, I. Labbe, S.J. Lilly, R. Mackenzie, P.A. Oesch, A. Weibel, S. Wuyts, M. Xiao, R. Bordoloi, R. Bouwens, P. van Dokkum, G. Illingworth, I. Kramarenko, M.V. Maseda, C. Mason, R.A. Meyer, E.J. Nelson, N.A. Reddy, I. Shivaei, R.A. Simcoe, M. Yue, The Astrophysical Journal 963 (2024).","ieee":"J. J. Matthee <i>et al.</i>, “Little Red Dots: An abundant population of faint active galactic nuclei at z ∼ 5 revealed by the EIGER and FRESCO JWST surveys,” <i>The Astrophysical Journal</i>, vol. 963, no. 2. IOP Publishing, 2024.","chicago":"Matthee, Jorryt J, Rohan P. Naidu, Gabriel Brammer, John Chisholm, Anna-Christina Eilers, Andy Goulding, Jenny Greene, et al. “Little Red Dots: An Abundant Population of Faint Active Galactic Nuclei at z ∼ 5 Revealed by the EIGER and FRESCO JWST Surveys.” <i>The Astrophysical Journal</i>. IOP Publishing, 2024. <a href=\"https://doi.org/10.3847/1538-4357/ad2345\">https://doi.org/10.3847/1538-4357/ad2345</a>.","apa":"Matthee, J. J., Naidu, R. P., Brammer, G., Chisholm, J., Eilers, A.-C., Goulding, A., … Yue, M. (2024). Little Red Dots: An abundant population of faint active galactic nuclei at z ∼ 5 revealed by the EIGER and FRESCO JWST surveys. <i>The Astrophysical Journal</i>. IOP Publishing. <a href=\"https://doi.org/10.3847/1538-4357/ad2345\">https://doi.org/10.3847/1538-4357/ad2345</a>"},"language":[{"iso":"eng"}],"keyword":["Space and Planetary Science","Astronomy and Astrophysics"],"date_published":"2024-03-07T00:00:00Z","external_id":{"isi":["001184703600001"]},"ddc":["550"],"fulldoi":"https://doi.org/10.3847/1538-4357/ad2345","has_accepted_license":"1","article_processing_charge":"Yes","type":"journal_article","day":"07","author":[{"id":"7439a258-f3c0-11ec-9501-9df22fe06720","first_name":"Jorryt J","full_name":"Matthee, Jorryt J","last_name":"Matthee","orcid":"0000-0003-2871-127X"},{"first_name":"Rohan P.","full_name":"Naidu, Rohan P.","last_name":"Naidu"},{"first_name":"Gabriel","full_name":"Brammer, Gabriel","last_name":"Brammer"},{"last_name":"Chisholm","full_name":"Chisholm, John","first_name":"John"},{"last_name":"Eilers","first_name":"Anna-Christina","full_name":"Eilers, Anna-Christina"},{"last_name":"Goulding","first_name":"Andy","full_name":"Goulding, Andy"},{"full_name":"Greene, Jenny","first_name":"Jenny","last_name":"Greene"},{"last_name":"Kashino","first_name":"Daichi","full_name":"Kashino, Daichi"},{"last_name":"Labbe","first_name":"Ivo","full_name":"Labbe, Ivo"},{"first_name":"Simon J.","full_name":"Lilly, Simon J.","last_name":"Lilly"},{"last_name":"Mackenzie","full_name":"Mackenzie, Ruari","first_name":"Ruari"},{"full_name":"Oesch, Pascal A.","first_name":"Pascal A.","last_name":"Oesch"},{"last_name":"Weibel","full_name":"Weibel, Andrea","first_name":"Andrea"},{"first_name":"Stijn","full_name":"Wuyts, Stijn","last_name":"Wuyts"},{"first_name":"Mengyuan","full_name":"Xiao, Mengyuan","last_name":"Xiao"},{"first_name":"Rongmon","full_name":"Bordoloi, Rongmon","last_name":"Bordoloi"},{"last_name":"Bouwens","first_name":"Rychard","full_name":"Bouwens, Rychard"},{"first_name":"Pieter","full_name":"van Dokkum, Pieter","last_name":"van Dokkum"},{"first_name":"Garth","full_name":"Illingworth, Garth","last_name":"Illingworth"},{"last_name":"Kramarenko","full_name":"Kramarenko, Ivan","first_name":"Ivan"},{"last_name":"Maseda","first_name":"Michael V.","full_name":"Maseda, Michael V."},{"first_name":"Charlotte","full_name":"Mason, Charlotte","last_name":"Mason"},{"last_name":"Meyer","full_name":"Meyer, Romain A.","first_name":"Romain A."},{"full_name":"Nelson, Erica J.","first_name":"Erica J.","last_name":"Nelson"},{"last_name":"Reddy","first_name":"Naveen A.","full_name":"Reddy, Naveen A."},{"last_name":"Shivaei","first_name":"Irene","full_name":"Shivaei, Irene"},{"full_name":"Simcoe, Robert A.","first_name":"Robert A.","last_name":"Simcoe"},{"last_name":"Yue","first_name":"Minghao","full_name":"Yue, Minghao"}],"publication_identifier":{"issn":["0004-637X"],"eissn":["1538-4357"]},"scopus_import":"1","supplementarymaterial":"yes","corr_author":"1","article_number":"129"},{"publication_identifier":{"eissn":["1538-4357"],"issn":["0004-637X"]},"scopus_import":"1","author":[{"full_name":"Greene, Jenny E.","first_name":"Jenny E.","last_name":"Greene"},{"last_name":"Labbe","full_name":"Labbe, Ivo","first_name":"Ivo"},{"last_name":"Goulding","first_name":"Andy D.","full_name":"Goulding, Andy D."},{"last_name":"Furtak","first_name":"Lukas J.","full_name":"Furtak, Lukas J."},{"full_name":"Chemerynska, Iryna","first_name":"Iryna","last_name":"Chemerynska"},{"full_name":"Kokorev, Vasily","first_name":"Vasily","last_name":"Kokorev"},{"last_name":"Dayal","first_name":"Pratika","full_name":"Dayal, Pratika"},{"full_name":"Volonteri, Marta","first_name":"Marta","last_name":"Volonteri"},{"full_name":"Williams, Christina C.","first_name":"Christina C.","last_name":"Williams"},{"last_name":"Wang","full_name":"Wang, Bingjie","first_name":"Bingjie"},{"last_name":"Setton","full_name":"Setton, David J.","first_name":"David J."},{"last_name":"Burgasser","first_name":"Adam J.","full_name":"Burgasser, Adam J."},{"full_name":"Bezanson, Rachel","first_name":"Rachel","last_name":"Bezanson"},{"last_name":"Atek","full_name":"Atek, Hakim","first_name":"Hakim"},{"first_name":"Gabriel","full_name":"Brammer, Gabriel","last_name":"Brammer"},{"last_name":"Cutler","first_name":"Sam E.","full_name":"Cutler, Sam E."},{"last_name":"Feldmann","first_name":"Robert","full_name":"Feldmann, Robert"},{"first_name":"Seiji","full_name":"Fujimoto, Seiji","last_name":"Fujimoto"},{"first_name":"Karl","full_name":"Glazebrook, Karl","last_name":"Glazebrook"},{"first_name":"Anna","full_name":"De Graaff, Anna","last_name":"De Graaff"},{"full_name":"Khullar, Gourav","first_name":"Gourav","last_name":"Khullar"},{"last_name":"Leja","first_name":"Joel","full_name":"Leja, Joel"},{"full_name":"Marchesini, Danilo","first_name":"Danilo","last_name":"Marchesini"},{"last_name":"Maseda","full_name":"Maseda, Michael V.","first_name":"Michael V."},{"id":"7439a258-f3c0-11ec-9501-9df22fe06720","first_name":"Jorryt J","full_name":"Matthee, Jorryt J","orcid":"0000-0003-2871-127X","last_name":"Matthee"},{"full_name":"Miller, Tim B.","first_name":"Tim B.","last_name":"Miller"},{"last_name":"Naidu","first_name":"Rohan P.","full_name":"Naidu, Rohan P."},{"full_name":"Nanayakkara, Themiya","first_name":"Themiya","last_name":"Nanayakkara"},{"first_name":"Pascal A.","full_name":"Oesch, Pascal A.","last_name":"Oesch"},{"last_name":"Pan","full_name":"Pan, Richard","first_name":"Richard"},{"full_name":"Papovich, Casey","first_name":"Casey","last_name":"Papovich"},{"last_name":"Price","full_name":"Price, Sedona H.","first_name":"Sedona H."},{"first_name":"Pieter","full_name":"Van Dokkum, Pieter","last_name":"Van Dokkum"},{"full_name":"Weaver, John R.","first_name":"John R.","last_name":"Weaver"},{"full_name":"Whitaker, Katherine E.","first_name":"Katherine E.","last_name":"Whitaker"},{"last_name":"Zitrin","full_name":"Zitrin, Adi","first_name":"Adi"}],"article_number":"39","supplementarymaterial":"no","type":"journal_article","article_processing_charge":"Yes","day":"01","has_accepted_license":"1","fulldoi":"https://doi.org/10.3847/1538-4357/ad1e5f","ddc":["550"],"language":[{"iso":"eng"}],"date_published":"2024-03-01T00:00:00Z","external_id":{"arxiv":["2309.05714"],"isi":["001184746500001"]},"arxiv":1,"doi":"10.3847/1538-4357/ad1e5f","publication_status":"published","citation":{"ista":"Greene JE, Labbe I, Goulding AD, Furtak LJ, Chemerynska I, Kokorev V, Dayal P, Volonteri M, Williams CC, Wang B, Setton DJ, Burgasser AJ, Bezanson R, Atek H, Brammer G, Cutler SE, Feldmann R, Fujimoto S, Glazebrook K, De Graaff A, Khullar G, Leja J, Marchesini D, Maseda MV, Matthee JJ, Miller TB, Naidu RP, Nanayakkara T, Oesch PA, Pan R, Papovich C, Price SH, Van Dokkum P, Weaver JR, Whitaker KE, Zitrin A. 2024. UNCOVER spectroscopy confirms the surprising ubiquity of active galactic nuclei in red sources at z &#62; 5. Astrophysical Journal. 964, 39.","ama":"Greene JE, Labbe I, Goulding AD, et al. UNCOVER spectroscopy confirms the surprising ubiquity of active galactic nuclei in red sources at z &#62; 5. <i>Astrophysical Journal</i>. 2024;964. doi:<a href=\"https://doi.org/10.3847/1538-4357/ad1e5f\">10.3847/1538-4357/ad1e5f</a>","mla":"Greene, Jenny E., et al. “UNCOVER Spectroscopy Confirms the Surprising Ubiquity of Active Galactic Nuclei in Red Sources at z &#62; 5.” <i>Astrophysical Journal</i>, vol. 964, 39, IOP Publishing, 2024, doi:<a href=\"https://doi.org/10.3847/1538-4357/ad1e5f\">10.3847/1538-4357/ad1e5f</a>.","chicago":"Greene, Jenny E., Ivo Labbe, Andy D. Goulding, Lukas J. Furtak, Iryna Chemerynska, Vasily Kokorev, Pratika Dayal, et al. “UNCOVER Spectroscopy Confirms the Surprising Ubiquity of Active Galactic Nuclei in Red Sources at z &#62; 5.” <i>Astrophysical Journal</i>. IOP Publishing, 2024. <a href=\"https://doi.org/10.3847/1538-4357/ad1e5f\">https://doi.org/10.3847/1538-4357/ad1e5f</a>.","apa":"Greene, J. E., Labbe, I., Goulding, A. D., Furtak, L. J., Chemerynska, I., Kokorev, V., … Zitrin, A. (2024). UNCOVER spectroscopy confirms the surprising ubiquity of active galactic nuclei in red sources at z &#62; 5. <i>Astrophysical Journal</i>. IOP Publishing. <a href=\"https://doi.org/10.3847/1538-4357/ad1e5f\">https://doi.org/10.3847/1538-4357/ad1e5f</a>","short":"J.E. Greene, I. Labbe, A.D. Goulding, L.J. Furtak, I. Chemerynska, V. Kokorev, P. Dayal, M. Volonteri, C.C. Williams, B. Wang, D.J. Setton, A.J. Burgasser, R. Bezanson, H. Atek, G. Brammer, S.E. Cutler, R. Feldmann, S. Fujimoto, K. Glazebrook, A. De Graaff, G. Khullar, J. Leja, D. Marchesini, M.V. Maseda, J.J. Matthee, T.B. Miller, R.P. Naidu, T. Nanayakkara, P.A. Oesch, R. Pan, C. Papovich, S.H. Price, P. Van Dokkum, J.R. Weaver, K.E. Whitaker, A. Zitrin, Astrophysical Journal 964 (2024).","ieee":"J. E. Greene <i>et al.</i>, “UNCOVER spectroscopy confirms the surprising ubiquity of active galactic nuclei in red sources at z &#62; 5,” <i>Astrophysical Journal</i>, vol. 964. IOP Publishing, 2024."},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"volume":964,"researchdata_availability":"no","year":"2024","acknowledgement":"J.E.G. and A.D.G acknowledge support from NSF/AAG grant No. 1007094, and J.E.G. also acknowledges support from NSF/AAG grant No. 1007052. A.Z. acknowledges support by grant No. 2020750 from the United States-Israel Binational Science Foundation (BSF) and grant No. 2109066 from the United States National Science Foundation (NSF), and by the Ministry of Science & Technology of Israel. The Cosmic Dawn Center is funded by the Danish National Research Foundation (DNRF) under grant No. 140. This work has received funding from the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract number MB22.00072, as well as from the Swiss National Science Foundation (SNSF) through project grant 200020_207349. P.D. acknowledges support from the NWO grant 016.VIDI.189.162 (\"ODIN\") and from the European Commission's and University of Groningen's CO-FUND Rosalind Franklin program. K.G. and T.N. acknowledge support from Australian Research Council Laureate Fellowship FL180100060. H.A. and I.C. acknowledge support from CNES, focused on the JWST mission, and the Programme National Cosmology and Galaxies (PNCG) of CNRS/INSU with INP and IN2P3, cofunded by CEA and CNES. R.P.N. acknowledges funding from JWST programs GO-1933 and GO-2279. Support for this work was provided by NASA through the NASA Hubble Fellowship grant HST-HF2-51515.001-A awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Incorporated, under NASA contract NAS5-26555. The research of C.C.W. is supported by NOIRLab, which is managed by the Association of Universities for Research in Astronomy (AURA) under a cooperative agreement with the National Science Foundation. B.W. acknowledges support from JWST-GO-02561.022-A. A.J.B. acknowledges funding support from NASA/ADAP grant 21-ADAP21-0187. Support for this work was provided by The Brinson Foundation through a Brinson Prize Fellowship grant. R.P.N. acknowledges support for this work provided by NASA through the NASA Hubble Fellowship grant HST-HF2-51515.001-A awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Incorporated, under NASA contract NAS5-26555. C.P. thanks Marsha and Ralph Schilling for the generous support of this research.","title":"UNCOVER spectroscopy confirms the surprising ubiquity of active galactic nuclei in red sources at z > 5","abstract":[{"lang":"eng","text":"The James Webb Space Telescope is revealing a new population of dust-reddened broad-line active galactic nuclei (AGN) at redshifts z ≳ 5. Here we present deep NIRSpec/Prism spectroscopy from the Cycle 1 Treasury program Ultradeep NIRSpec and NIRCam ObserVations before the Epoch of Reionization (UNCOVER) of 15 AGN candidates selected to be compact, with red continua in the rest-frame optical but with blue slopes in the UV. From NIRCam photometry alone, they could have been dominated by dusty star formation or an AGN. Here we show that the majority of the compact red sources in UNCOVER are dust-reddened AGN: 60% show definitive evidence for broad-line Hα with a FWHM > 2000 km s −1, 20% of the current data are inconclusive, and 20% are brown dwarf stars. We propose an updated photometric criterion to select red z > 5 AGN that excludes brown dwarfs and is expected to yield >80% AGN. Remarkably, among all zphot > 5 galaxies with F277W – F444W > 1 in UNCOVER at least 33% are AGN regardless of compactness, climbing to at least 80% AGN for sources with F277W – F444W > 1.6. The confirmed AGN have black hole masses of 107–109M⊙. While their UV luminosities (−16 > MUV > −20 AB mag) are low compared to UV-selected AGN at these epochs, consistent with percent-level scattered AGN light or low levels of unobscured star formation, the inferred bolometric luminosities are typical of 107–109M⊙ black holes radiating at ∼10%–40% the Eddington limit. The number densities are surprisingly high at ∼10−5 Mpc−3 mag−1, 100 times more common than the faintest UV-selected quasars, while accounting for ∼1% of the UV-selected galaxies. While their UV faintness suggests they may not contribute strongly to reionization, their ubiquity poses challenges to models of black hole growth."}],"quality_controlled":"1","publisher":"IOP Publishing","status":"public","DOAJ_listed":"1","_id":"15170","month":"03","date_created":"2024-03-24T23:00:59Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","department":[{"_id":"JoMa"}],"das_tickbox":"0","oa":1,"date_updated":"2026-10-15T09:15:28Z","isi":1,"file":[{"file_name":"2024_AstrophysicalJourn_Greene.pdf","creator":"dernst","success":1,"date_updated":"2024-03-25T08:02:43Z","file_id":"15176","access_level":"open_access","date_created":"2024-03-25T08:02:43Z","content_type":"application/pdf","file_size":2700137,"checksum":"389a880e176799d5c062ea7cb82d08c9","relation":"main_file"}],"publication":"Astrophysical Journal","oa_version":"Published Version","intvolume":"       964","file_date_updated":"2024-03-25T08:02:43Z"},{"language":[{"iso":"eng"}],"date_published":"2024-07-01T00:00:00Z","external_id":{"isi":["001257903200001"],"arxiv":["2405.01473"]},"arxiv":1,"has_accepted_license":"1","fulldoi":"https://doi.org/10.3847/2041-8213/ad55f7","ddc":["520"],"type":"journal_article","article_processing_charge":"Yes","day":"01","scopus_import":"1","publication_identifier":{"eissn":["2041-8213"],"issn":["2041-8205"]},"author":[{"last_name":"Wang","full_name":"Wang, Bingjie","first_name":"Bingjie"},{"last_name":"Leja","first_name":"Joel","full_name":"Leja, Joel"},{"first_name":"Anna","full_name":"De Graaff, Anna","last_name":"De Graaff"},{"full_name":"Brammer, Gabriel B.","first_name":"Gabriel B.","last_name":"Brammer"},{"first_name":"Andrea","full_name":"Weibel, Andrea","last_name":"Weibel"},{"first_name":"Pieter","full_name":"Van Dokkum, Pieter","last_name":"Van Dokkum"},{"last_name":"Baggen","first_name":"Josephine F.W.","full_name":"Baggen, Josephine F.W."},{"first_name":"Katherine A.","full_name":"Suess, Katherine A.","last_name":"Suess"},{"full_name":"Greene, Jenny E.","first_name":"Jenny E.","last_name":"Greene"},{"full_name":"Bezanson, Rachel","first_name":"Rachel","last_name":"Bezanson"},{"first_name":"Nikko J.","full_name":"Cleri, Nikko J.","last_name":"Cleri"},{"last_name":"Hirschmann","full_name":"Hirschmann, Michaela","first_name":"Michaela"},{"first_name":"Ivo","full_name":"Labbé, Ivo","last_name":"Labbé"},{"first_name":"Jorryt J","id":"7439a258-f3c0-11ec-9501-9df22fe06720","full_name":"Matthee, Jorryt J","last_name":"Matthee","orcid":"0000-0003-2871-127X"},{"full_name":"Mcconachie, Ian","first_name":"Ian","last_name":"Mcconachie"},{"last_name":"Naidu","first_name":"Rohan P.","full_name":"Naidu, Rohan P."},{"last_name":"Nelson","full_name":"Nelson, Erica","first_name":"Erica"},{"last_name":"Oesch","full_name":"Oesch, Pascal A.","first_name":"Pascal A."},{"last_name":"Setton","first_name":"David J.","full_name":"Setton, David J."},{"first_name":"Christina C.","full_name":"Williams, Christina C.","last_name":"Williams"}],"article_number":"L13","supplementarymaterial":"yes","das_tickbox":"0","oa":1,"isi":1,"date_updated":"2026-10-15T09:10:55Z","file":[{"access_level":"open_access","date_created":"2024-07-16T06:24:29Z","content_type":"application/pdf","checksum":"bb1a6725586df12e745d091b5778bb2b","file_size":3273303,"relation":"main_file","file_name":"2024_AstrophysicalJourn_Wang.pdf","creator":"dernst","success":1,"date_updated":"2024-07-16T06:24:29Z","file_id":"17243"}],"publication":"Astrophysical Journal Letters","oa_version":"Published Version","intvolume":"       969","file_date_updated":"2024-07-16T06:24:29Z","DOAJ_listed":"1","_id":"17234","month":"07","date_created":"2024-07-14T22:01:11Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","department":[{"_id":"JoMa"}],"acknowledgement":"We thank the anonymous referee for the helpful comments. B.W. and J.L. acknowledge support from JWST-GO04233.009-A. The Cosmic Dawn Center is funded by the Danish National Research Foundation (DNRF) under grant No. 140. This research was supported by the International Space Science Institute (ISSI) in Bern, through ISSI International Team project No. 562 (First Light at Cosmic Dawn: Exploiting the James Webb Space Telescope Revolution). This work is based in part on observations made with the NASA/ESA/CSA James Webb Space Telescope. The data were obtained from the Mikulski Archive for Space Telescopes at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-03127 for JWST. The JWST data presented in this Letter were obtained from the Mikulski Archive for Space Telescopes (MAST) at the Space Telescope Science Institute. The specific observations analyzed can be accessed via doi:10.17909/3a4n-9p88. Computations for this research were performed on the Pennsylvania State University’s Institute for Computational and Data Sciences’ Roar supercomputer. This publication made use of the NASA Astrophysical Data System for bibliographic information. \r\nFacilities: HST (ACS, WFC3), JWST (NIRCam, NIRSpec). Software: Astropy (Astropy Collaboration et al. 2013, 2018, 2022), dynesty (Speagle 2020), EAzY (Brammer et al. 2008),\r\nemcee (Foreman-Mackey et al. 2013), Matplotlib (Hunter 2007), msaexp (Brammer 2023b), msafit (de Graaff et al. 2024a), NumPy (Harris et al. 2020), Prospector (Johnson et al. 2021), Python-FSPS (Johnson et al. 2023).","abstract":[{"text":"The identification of red, apparently massive galaxies at z > 7 in early James Webb Space Telescope (JWST) photometry suggests a strongly accelerated time line compared to standard models of galaxy growth. A major uncertainty in the interpretation is whether the red colors are caused by evolved stellar populations, dust, or other effects such as emission lines or active galactic nuclei (AGNs). Here we show that three of the massive galaxy candidates at z = 6.7–8.4 have prominent Balmer breaks in JWST/NIRSpec spectroscopy from the RUBIES program. The Balmer breaks demonstrate unambiguously that stellar emission dominates at λrest = 0.4 μm and require formation histories extending hundreds of millions of years into the past in galaxies only 600–800 Myr after the big bang. Two of the three galaxies also show broad Balmer lines, with Hβ FWHM > 2500 km s−1, suggesting that dust-reddened AGNs contribute to, or even dominate, the spectral energy distributions of these galaxies at λrest ≳ 0.6 μm. All three galaxies have relatively narrow [O iii] lines, seemingly ruling out a high-mass interpretation if the lines arise in dynamically relaxed, inclined disks. Yet the inferred masses also remain highly uncertain. We model the high-quality spectra using Prospector to decompose the continuum into stellar and AGN components and explore limiting cases in stellar/AGN contribution. This produces a wide range of possible stellar masses, spanning M⋆ ∼ 109−1011M⊙. Nevertheless, all fits suggest a very early and rapid formation, most of which follow with a truncation in star formation. Potential origins and evolutionary tracks for these objects are discussed, from the cores of massive galaxies to low-mass galaxies with overmassive black holes. Intriguingly, we find all of these explanations to be incomplete; deeper and redder data are needed to understand the physics of these systems.","lang":"eng"}],"title":"RUBIES: Evolved stellar populations with extended formation histories at z ∼ 7-8 in candidate massive galaxies identified with JWST/NIRSpec","quality_controlled":"1","publisher":"IOP Publishing","issue":"1","status":"public","doi":"10.3847/2041-8213/ad55f7","citation":{"ieee":"B. Wang <i>et al.</i>, “RUBIES: Evolved stellar populations with extended formation histories at z ∼ 7-8 in candidate massive galaxies identified with JWST/NIRSpec,” <i>Astrophysical Journal Letters</i>, vol. 969, no. 1. IOP Publishing, 2024.","short":"B. Wang, J. Leja, A. De Graaff, G.B. Brammer, A. Weibel, P. Van Dokkum, J.F.W. Baggen, K.A. Suess, J.E. Greene, R. Bezanson, N.J. Cleri, M. Hirschmann, I. Labbé, J.J. Matthee, I. Mcconachie, R.P. Naidu, E. Nelson, P.A. Oesch, D.J. Setton, C.C. Williams, Astrophysical Journal Letters 969 (2024).","apa":"Wang, B., Leja, J., De Graaff, A., Brammer, G. B., Weibel, A., Van Dokkum, P., … Williams, C. C. (2024). RUBIES: Evolved stellar populations with extended formation histories at z ∼ 7-8 in candidate massive galaxies identified with JWST/NIRSpec. <i>Astrophysical Journal Letters</i>. IOP Publishing. <a href=\"https://doi.org/10.3847/2041-8213/ad55f7\">https://doi.org/10.3847/2041-8213/ad55f7</a>","chicago":"Wang, Bingjie, Joel Leja, Anna De Graaff, Gabriel B. Brammer, Andrea Weibel, Pieter Van Dokkum, Josephine F.W. Baggen, et al. “RUBIES: Evolved Stellar Populations with Extended Formation Histories at z ∼ 7-8 in Candidate Massive Galaxies Identified with JWST/NIRSpec.” <i>Astrophysical Journal Letters</i>. IOP Publishing, 2024. <a href=\"https://doi.org/10.3847/2041-8213/ad55f7\">https://doi.org/10.3847/2041-8213/ad55f7</a>.","mla":"Wang, Bingjie, et al. “RUBIES: Evolved Stellar Populations with Extended Formation Histories at z ∼ 7-8 in Candidate Massive Galaxies Identified with JWST/NIRSpec.” <i>Astrophysical Journal Letters</i>, vol. 969, no. 1, L13, IOP Publishing, 2024, doi:<a href=\"https://doi.org/10.3847/2041-8213/ad55f7\">10.3847/2041-8213/ad55f7</a>.","ama":"Wang B, Leja J, De Graaff A, et al. RUBIES: Evolved stellar populations with extended formation histories at z ∼ 7-8 in candidate massive galaxies identified with JWST/NIRSpec. <i>Astrophysical Journal Letters</i>. 2024;969(1). doi:<a href=\"https://doi.org/10.3847/2041-8213/ad55f7\">10.3847/2041-8213/ad55f7</a>","ista":"Wang B, Leja J, De Graaff A, Brammer GB, Weibel A, Van Dokkum P, Baggen JFW, Suess KA, Greene JE, Bezanson R, Cleri NJ, Hirschmann M, Labbé I, Matthee JJ, Mcconachie I, Naidu RP, Nelson E, Oesch PA, Setton DJ, Williams CC. 2024. RUBIES: Evolved stellar populations with extended formation histories at z ∼ 7-8 in candidate massive galaxies identified with JWST/NIRSpec. Astrophysical Journal Letters. 969(1), L13."},"publication_status":"published","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","volume":969,"researchdata_availability":"yes"},{"scopus_import":"1","publication_identifier":{"issn":["0004-6361"],"eissn":["1432-0746"]},"author":[{"last_name":"Torralba-Torregrosa","full_name":"Torralba-Torregrosa, Alberto","first_name":"Alberto"},{"orcid":"0000-0003-2871-127X","last_name":"Matthee","id":"7439a258-f3c0-11ec-9501-9df22fe06720","first_name":"Jorryt J","full_name":"Matthee, Jorryt J"},{"full_name":"Naidu, Rohan P.","first_name":"Rohan P.","last_name":"Naidu"},{"last_name":"Mackenzie","full_name":"Mackenzie, Ruari","first_name":"Ruari"},{"last_name":"Pezzulli","full_name":"Pezzulli, Gabriele","first_name":"Gabriele"},{"last_name":"Hutter","full_name":"Hutter, Anne","first_name":"Anne"},{"last_name":"Arnalte-Mur","first_name":"Pablo","full_name":"Arnalte-Mur, Pablo"},{"last_name":"Gurung-López","full_name":"Gurung-López, Siddhartha","first_name":"Siddhartha"},{"first_name":"Sandro","full_name":"Tacchella, Sandro","last_name":"Tacchella"},{"last_name":"Oesch","full_name":"Oesch, Pascal","first_name":"Pascal"},{"last_name":"Kashino","first_name":"Daichi","full_name":"Kashino, Daichi"},{"last_name":"Conroy","first_name":"Charlie","full_name":"Conroy, Charlie"},{"full_name":"Sobral, David","first_name":"David","last_name":"Sobral"}],"article_number":"A44","supplementarymaterial":"yes","type":"journal_article","article_processing_charge":"Yes (in subscription journal)","day":"01","has_accepted_license":"1","ddc":["520"],"fulldoi":"https://doi.org/10.1051/0004-6361/202450318","language":[{"iso":"eng"}],"date_published":"2024-09-01T00:00:00Z","external_id":{"isi":["001303205700016"],"arxiv":["2404.10040"]},"arxiv":1,"doi":"10.1051/0004-6361/202450318","publication_status":"published","citation":{"ieee":"A. Torralba-Torregrosa <i>et al.</i>, “Anatomy of an ionized bubble: NIRCam grism spectroscopy of the z = 6.6 double-peaked Lyman- α emitter COLA1 and its environment,” <i>Astronomy &#38; Astrophysics</i>, vol. 689. EDP Sciences, 2024.","short":"A. Torralba-Torregrosa, J.J. Matthee, R.P. Naidu, R. Mackenzie, G. Pezzulli, A. Hutter, P. Arnalte-Mur, S. Gurung-López, S. Tacchella, P. Oesch, D. Kashino, C. Conroy, D. Sobral, Astronomy &#38; Astrophysics 689 (2024).","apa":"Torralba-Torregrosa, A., Matthee, J. J., Naidu, R. P., Mackenzie, R., Pezzulli, G., Hutter, A., … Sobral, D. (2024). Anatomy of an ionized bubble: NIRCam grism spectroscopy of the z = 6.6 double-peaked Lyman- α emitter COLA1 and its environment. <i>Astronomy &#38; Astrophysics</i>. EDP Sciences. <a href=\"https://doi.org/10.1051/0004-6361/202450318\">https://doi.org/10.1051/0004-6361/202450318</a>","chicago":"Torralba-Torregrosa, Alberto, Jorryt J Matthee, Rohan P. Naidu, Ruari Mackenzie, Gabriele Pezzulli, Anne Hutter, Pablo Arnalte-Mur, et al. “Anatomy of an Ionized Bubble: NIRCam Grism Spectroscopy of the z = 6.6 Double-Peaked Lyman- α Emitter COLA1 and Its Environment.” <i>Astronomy &#38; Astrophysics</i>. EDP Sciences, 2024. <a href=\"https://doi.org/10.1051/0004-6361/202450318\">https://doi.org/10.1051/0004-6361/202450318</a>.","mla":"Torralba-Torregrosa, Alberto, et al. “Anatomy of an Ionized Bubble: NIRCam Grism Spectroscopy of the z = 6.6 Double-Peaked Lyman- α Emitter COLA1 and Its Environment.” <i>Astronomy &#38; Astrophysics</i>, vol. 689, A44, EDP Sciences, 2024, doi:<a href=\"https://doi.org/10.1051/0004-6361/202450318\">10.1051/0004-6361/202450318</a>.","ama":"Torralba-Torregrosa A, Matthee JJ, Naidu RP, et al. Anatomy of an ionized bubble: NIRCam grism spectroscopy of the z = 6.6 double-peaked Lyman- α emitter COLA1 and its environment. <i>Astronomy &#38; Astrophysics</i>. 2024;689. doi:<a href=\"https://doi.org/10.1051/0004-6361/202450318\">10.1051/0004-6361/202450318</a>","ista":"Torralba-Torregrosa A, Matthee JJ, Naidu RP, Mackenzie R, Pezzulli G, Hutter A, Arnalte-Mur P, Gurung-López S, Tacchella S, Oesch P, Kashino D, Conroy C, Sobral D. 2024. Anatomy of an ionized bubble: NIRCam grism spectroscopy of the z = 6.6 double-peaked Lyman- α emitter COLA1 and its environment. Astronomy &#38; Astrophysics. 689, A44."},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2024","volume":689,"researchdata_availability":"no","acknowledgement":"The authors acknowledge the financial support from the MICIU with funding from the European Union NextGenerationEU and Generalitat Valenciana in the call Programa de Planes Complementarios de I+D+i (PRTR 2022) Project (VAL-JPAS), reference ASFAE/2022/025. This work has been funded by project PID2019-109592GBI00/AEI/10.13039/501100011033 from the Spanish Ministerio de Ciencia e Innovación (MCIN)-Agencia Estatal de Investigación, by the Project of Excellence Prometeo/2020/085 from the Conselleria d’Innovació Universitats, Ciència i Societat Digital de la Generalitat Valenciana. It has also be funded by the Project of Excellence Prometeo/2020/085 from the Conselleria d’Educació, Universitats, i Ocupació de la Generalitat Valenciana. Funded by the European Union (ERC, AGENTS, 101076224). Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council. Neither the European Union nor the granting authority can be held responsible for them. ST acknowledges support by the Royal Society Research Grant G125142. AH acknowledges support by the VILLUM FONDEN under grant 37459. The Cosmic Dawn Center (DAWN) is funded by the Danish National Research Foundation under grant DNRF140. We acknowledge funding from JWST program GO-1933. Support for this work was provided by NASA through the NASA Hubble Fellowship grant HST-HF2-51515.001-A awarded by the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Incorporated, under NASA contract NAS5-26555. This work has received funding from the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract number MB22.00072, as well as from the Swiss National Science Foundation (SNSF) through project grant 200020_207349. This work is based on observations made with the NASA/ESA/CSA James Webb Space Telescope. The data were obtained from the Mikulski Archive for Space Telescopes at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-03127 for JWST. These observations are associated with program # 1933. The specific observations analyzed can be accessed via https://doi.org/10.17909/s9ht-7n34.","abstract":[{"lang":"eng","text":"The increasingly neutral intergalactic gas at z > 6 impacts the Lyman-α (Lyα) flux observed from galaxies. One luminous galaxy, COLA1, stands out because of its unique double-peaked Lyα line at z = 6.6, unseen in any simulation of reionization. Here, we present JWST/NIRCam wide-field slitless spectroscopy in a 21 arcmin2 field centered on COLA1. We find 141 galaxies spectroscopically selected through the [O III] doublet at 5.35 < z < 6.95, with 40 of these sources showing Hβ. For COLA1, we additionally detect [O III]4363 as well as Hγ. We measure a systemic redshift of z = 6.5917 for COLA1, confirming the classical double-peak nature of the Lyα profile. This implies that it resides in a highly ionized bubble and that it is leaking ionizing photons with a high escape fraction of fesc(LyC) = 20–50%, making it a prime laboratory to study Lyman continuum escape in the Epoch of Reionization. COLA1 shows all the signs of a prolific ionizer with a Lyα escape fraction of 81 ± 5%, Balmer decrement indicating no dust, a steep UV slope (βUV = −3.2 ± 0.4), and a star-formation surface density ≳10× that of typical galaxies at similar redshift. We detect five galaxies in COLA1’s close environment (Δz < 0.02). Exploiting the high spectroscopic completeness inherent to grism surveys, and using mock simulations that fully mimic the selection function, we show that the number of detected companions is very typical for a normal similarly UV-bright (MUV ∼ −21.3) galaxy – that is, the ionized bubble around COLA1 is unlikely to be due to an excessively large over-density. Instead, the measured ionizing properties suggest that COLA1 by itself might be powering the bubble required to explain its double-peaked Lyα profile (Rion ≈ 0.7 pMpc), with only minor contributions from detected neighbors (−19.5 ≲ MUV ≲ −17.5)."}],"title":"Anatomy of an ionized bubble: NIRCam grism spectroscopy of the z = 6.6 double-peaked Lyman- α emitter COLA1 and its environment","quality_controlled":"1","status":"public","publisher":"EDP Sciences","_id":"17889","month":"09","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_created":"2024-09-08T22:01:11Z","department":[{"_id":"JoMa"}],"article_type":"original","das_tickbox":"0","file":[{"date_updated":"2024-09-11T07:35:00Z","file_id":"18055","success":1,"creator":"dernst","file_name":"2024_AstronomyAstrophysics_TorralbaTorregrosa.pdf","relation":"main_file","file_size":6225413,"checksum":"a6c0df287c75e8929db9f42badeac859","content_type":"application/pdf","date_created":"2024-09-11T07:35:00Z","access_level":"open_access"}],"isi":1,"date_updated":"2026-10-15T09:15:07Z","oa":1,"publication":"Astronomy & Astrophysics","oa_version":"Published Version","file_date_updated":"2024-09-11T07:35:00Z","intvolume":"       689"}]