[{"type":"journal_article","oa_version":"Published Version","article_type":"original","abstract":[{"text":"Aims. We investigate signatures of pressure extrema on global oscillations in discs.\r\n\r\nMethods. We used the framework of wave topology to establish a generalised local dispersion relation that includes pressure gradients. We highlight the influence of a previously unrecognised epicyclic–acoustic frequency, and we derive an analytical criterion for the existence of a branch of modes transiting between the inertial and the pressure bands, known as topological modes.\r\n\r\nResults. We find that pressure extrema consist of wave guides in which such topological modes propagate. The fundamental mode trapped at a pressure bump can propagate at any frequency, allowing it to resonate with any temporal forcing. Conversely, the fundamental mode associated with a pressure dip propagates at any vertical phase velocity. These specific features make them attractive candidates for future discoseismology.","lang":"eng"}],"title":"Radial modes of pressure bumps and dips in astrophysical discs","_id":"23047","date_created":"2026-10-04T22:01:54Z","author":[{"full_name":"Leclerc, Armand","last_name":"Leclerc","first_name":"Armand","id":"2a1fb1fc-f373-11ef-901a-87cee43a1217"},{"last_name":"Laibe","first_name":"Guillaume","full_name":"Laibe, Guillaume"},{"first_name":"Elliot","last_name":"Lynch","full_name":"Lynch, Elliot"},{"full_name":"Perez, Nicolas","last_name":"Perez","first_name":"Nicolas"}],"status":"public","date_updated":"2026-10-20T08:14:41Z","supplementarymaterial":"yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","PlanS_conform":"1","citation":{"chicago":"Leclerc, Armand, Guillaume Laibe, Elliot Lynch, and Nicolas Perez. “Radial Modes of Pressure Bumps and Dips in Astrophysical Discs.” <i>Astronomy &#38; Astrophysics</i>. EDP Sciences, 2026. <a href=\"https://doi.org/10.1051/0004-6361/202558428\">https://doi.org/10.1051/0004-6361/202558428</a>.","apa":"Leclerc, A., Laibe, G., Lynch, E., &#38; Perez, N. (2026). Radial modes of pressure bumps and dips in astrophysical discs. <i>Astronomy &#38; Astrophysics</i>. EDP Sciences. <a href=\"https://doi.org/10.1051/0004-6361/202558428\">https://doi.org/10.1051/0004-6361/202558428</a>","ama":"Leclerc A, Laibe G, Lynch E, Perez N. Radial modes of pressure bumps and dips in astrophysical discs. <i>Astronomy &#38; Astrophysics</i>. 2026;708. doi:<a href=\"https://doi.org/10.1051/0004-6361/202558428\">10.1051/0004-6361/202558428</a>","mla":"Leclerc, Armand, et al. “Radial Modes of Pressure Bumps and Dips in Astrophysical Discs.” <i>Astronomy &#38; Astrophysics</i>, vol. 708, A355, EDP Sciences, 2026, doi:<a href=\"https://doi.org/10.1051/0004-6361/202558428\">10.1051/0004-6361/202558428</a>.","short":"A. Leclerc, G. Laibe, E. Lynch, N. Perez, Astronomy &#38; Astrophysics 708 (2026).","ieee":"A. Leclerc, G. Laibe, E. Lynch, and N. Perez, “Radial modes of pressure bumps and dips in astrophysical discs,” <i>Astronomy &#38; Astrophysics</i>, vol. 708. EDP Sciences, 2026.","ista":"Leclerc A, Laibe G, Lynch E, Perez N. 2026. Radial modes of pressure bumps and dips in astrophysical discs. Astronomy &#38; Astrophysics. 708, A355."},"DOAJ_listed":"1","project":[{"grant_number":"101165631","name":"Unveiling the mysteries of stellar dynamics: a pioneering journey in magnetoasteroseismology","_id":"914d8549-16d5-11f0-9cad-bbe6324c93a9"}],"acknowledgement":"AL was funded by Contrat Doctoral Spécifique Normaliens during this work. GL, EL and NP acknowledge funding from\r\nERC CoG project PODCAST No 864965. AL acknowledges funding\r\nfrom ERC StG project Calcifer No 101165631. We used Mathematica\r\n(Wolfram Research Inc, 2024).\r\n","publication_identifier":{"issn":["0004-6361"],"eissn":["1432-0746"]},"publication":"Astronomy & Astrophysics","scopus_import":"1","language":[{"iso":"eng"}],"OA_type":"diamond","OA_place":"publisher","publication_status":"published","quality_controlled":"1","year":"2026","intvolume":"       708","ddc":["520"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"das_tickbox":"1","fulldoi":"https://doi.org/10.1051/0004-6361/202558428","arxiv":1,"has_accepted_license":"1","date_published":"2026-04-01T00:00:00Z","file_date_updated":"2026-10-07T12:13:55Z","article_processing_charge":"No","keyword":["accretion","accretion disks","hydrodynamics","instabilities","waves","protoplanetary disks"],"external_id":{"arxiv":["2512.05737"]},"publisher":"EDP Sciences","department":[{"_id":"LiBu"}],"month":"04","day":"01","dataavailabilitystatement":"The scripts written and used for numerical calculations\r\nare accessible at https://github.com/ArmandLeclerc/\r\nradialStratifDisc.","corr_author":"1","oa":1,"volume":708,"doi":"10.1051/0004-6361/202558428","researchdata_availability":"no","file":[{"file_id":"23075","content_type":"application/pdf","date_updated":"2026-10-07T12:13:55Z","file_name":"2026_AstronomyAstrophysics_Leclerc.pdf","file_size":10533299,"success":1,"date_created":"2026-10-07T12:13:55Z","checksum":"868c1be53a4f3e0cf2852a723fcacd08","access_level":"open_access","creator":"dernst","relation":"main_file"}],"article_number":"A355"},{"doi":"10.15479/AT-ISTA-22744","file":[{"file_id":"22767","file_size":27430796,"file_name":"2026_Javoor_Manjunath_Thesis.docx","date_updated":"2026-08-27T12:48:42Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_created":"2026-08-26T12:02:47Z","checksum":"f9c2847df9f1ac5a3d60c06b3b81a450","creator":"mjavoor","relation":"source_file","access_level":"closed"},{"file_id":"22768","embargo_to":"open_access","file_size":19489230,"file_name":"2026_Javoor_Manjunath_Thesis.pdf","embargo":"2027-08-21","content_type":"application/pdf","date_updated":"2026-08-26T12:02:46Z","checksum":"8e9b4c0fcafbccf5c3796eacc9134a08","date_created":"2026-08-26T12:02:46Z","creator":"mjavoor","relation":"main_file","access_level":"closed"}],"OA_embargo":"12","day":"21","corr_author":"1","doi_confirm":"1","publisher":"Institute of Science and Technology Austria ","department":[{"_id":"GradSch"},{"_id":"FlSc"},{"_id":"MiSi"}],"month":"08","has_accepted_license":"1","date_published":"2026-08-21T00:00:00Z","file_date_updated":"2026-08-27T12:48:42Z","alternative_title":["ISTA Thesis"],"article_processing_charge":"No","keyword":["Actin cytoskeleton","Cell migration","cryo-electron tomography"],"degree_awarded":"PhD","fulldoi":"https://doi.org/10.15479/AT-ISTA-22744","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"ddc":["570"],"publication_status":"published","year":"2026","OA_place":"publisher","language":[{"iso":"eng"}],"publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-090-9 "]},"acknowledgement":"This work was supported by the ERC StG grant ActinID (PRA01221F1049A) awarded to Florian\r\nSchur, the ERC-SyG grant Pushing from within (P01071793) awarded to Michael Sixt, and by ISTA.\r\nI would like to thank the Scientific Service Units at ISTA for their essential support throughout\r\nthis work. In particular, I am grateful to the Electron Microscopy Facility, Imaging and Optics\r\nFacility, Zebrafish Facility, Scientific Computing Facility, and Lab Support Facility for their services,\r\nand technical support, all of which were important for the successful completion of this project.","related_material":{"record":[{"status":"public","id":"12334","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"21762"},{"relation":"part_of_dissertation","id":"12421","status":"public"},{"relation":"part_of_dissertation","id":"19795","status":"public"}]},"page":"121","citation":{"chicago":"Javoor, Manjunath. “Large-Scale Imaging of Cellular Actin Networks at Single Filament Resolution Using Montage Cryo-Electron Tomography.” Institute of Science and Technology Austria , 2026. <a href=\"https://doi.org/10.15479/AT-ISTA-22744\">https://doi.org/10.15479/AT-ISTA-22744</a>.","apa":"Javoor, M. (2026). <i>Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography</i>. Institute of Science and Technology Austria . <a href=\"https://doi.org/10.15479/AT-ISTA-22744\">https://doi.org/10.15479/AT-ISTA-22744</a>","ama":"Javoor M. Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography. 2026. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-22744\">10.15479/AT-ISTA-22744</a>","mla":"Javoor, Manjunath. <i>Large-Scale Imaging of Cellular Actin Networks at Single Filament Resolution Using Montage Cryo-Electron Tomography</i>. Institute of Science and Technology Austria , 2026, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-22744\">10.15479/AT-ISTA-22744</a>.","ista":"Javoor M. 2026. Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography. Institute of Science and Technology Austria .","short":"M. Javoor, Large-Scale Imaging of Cellular Actin Networks at Single Filament Resolution Using Montage Cryo-Electron Tomography, Institute of Science and Technology Austria , 2026.","ieee":"M. Javoor, “Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography,” Institute of Science and Technology Austria , 2026."},"project":[{"name":"A molecular atlas of Actin filament IDentities in the cell motility machinery","_id":"bd980d18-d553-11ed-ba76-ceaa645c97eb","grant_number":"101076260"},{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"}],"date_updated":"2026-10-20T08:55:33Z","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","title":"Large-scale imaging of cellular actin networks at single filament resolution using montage cryo-electron tomography","_id":"22744","author":[{"id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","first_name":"Manjunath","last_name":"Javoor","full_name":"Javoor, Manjunath","orcid":"0000-0003-2311-2112"}],"status":"public","date_created":"2026-08-21T09:11:04Z","type":"dissertation","supervisor":[{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","last_name":"Schur","orcid":"0000-0003-4790-8078","full_name":"Schur, Florian KM"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"oa_version":"None"},{"OA_type":"gold","OA_place":"publisher","language":[{"iso":"eng"}],"publication":"Cell Reports Physical Science","issue":"4","scopus_import":"1","publication_identifier":{"eissn":["2666-3864"]},"acknowledgement":"This work is a collaborative effort of the titled authors as part of the Origin of Life Early Career Network (OoLEN). We chose to add OoLEN as the first author to give a better representation of this team effort, rather than listing any single author as the first author. We hope such a thing can be adopted by others. We indicate that authors 2–9 (S.A., C.B., C. Blanco, D.B., A.C.-R., C.M., O.M., Z.P., and A.V.D.) have made a more distinct contribution. All authors are listed alphabetically by their last names. We would like to acknowledge all current and past members of OoLEN for their contributions to our community. In particular, we would like to acknowledge Evrim Fer, who helped with molecular phylogenetics. We would like to thank the anonymous referees for reviewing Parts 1 and 2 of this manuscript; this work was significantly improved through their feedback. S.A. acknowledges support from NASA through the postdoctoral Program at GSFC. C. Bautista acknowledges support from “la Caixa” Foundation (ID 100010434) under agreement (LCF/BQ/AA16/11580051) and by the Fonds de recherche du Québec Nature et technologies (FRQNT) (#274987). C. Blanco acknowledges support from NASA under award 80NSSC21K0595. D.B. acknowledges support from Centre national d'études spatiales (CNES) and postdoctoral support from LGPM-CentralSupélec and NASA under award 80NSSC23K1477. E. Camprubi acknowledges support from UT System for a STARs award. A.C.-R. acknowledges funding from the Natural Sciences and Engineering Research Council of Canada (grant number RGPIN/05278–2018), the Fonds de recherche Nature et Technologies of Québec (grant number 314488), and the Fondation J. Armand Bombardier Excellence Scholarship. A.C.-R.’s research was supported by an appointment to the NASA Postdoctoral Program from the NASA Astrobiology Program administered by Oak Ridge Associated Universities under contract with NASA. S.F.J. acknowledges support from “la Caixa” Foundation (ID 100010434) and from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska Curie grant agreement no. 847648 (the fellowship code is LCF/BQ/PI21/11830015). T.Z.J. acknowledges support from Japan Society for the Promotion of Science (JSPS) grants-in-aid 18K14354 and 21K14746, a Tokyo Institute of Technology Yoshinori Ohsumi Fund for Fundamental Research, the Mizuho Foundation for the Promotion of Sciences, and by the Temporary Assistant Program by the DE&I Section of Science Tokyo. A.K. acknowledges support from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant agreement no. 101068029. C.M. acknowledges support from NASA through the postdoctoral Fellowship Program. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of NASA. O.M. acknowledges support from The John Templeton Foundation (#62828) and the Foundation for Science and Technology (2023.05971.CEECIND). B.K.D.P. acknowledges support from the NSERC Banting Postdoctoral Fellowship. K.P. acknowledges financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy EXC 2181/1 - 390900948 (the Heidelberg STRUCTURES Excellence Cluster) and is a fellow of the International Max Planck Research School for Astronomy and Cosmic Physics at the University of Heidelberg (IMPRS-HD).","DOAJ_listed":"1","citation":{"ama":"Asche S, Bautista C, Blanco C, et al. What it takes to solve the origin of life: An integrated review. Part 1–Experimental methods and data repositories. <i>Cell Reports Physical Science</i>. 2026;7(4). doi:<a href=\"https://doi.org/10.1016/j.xcrp.2026.103212\">10.1016/j.xcrp.2026.103212</a>","mla":"Asche, Silke, et al. “What It Takes to Solve the Origin of Life: An Integrated Review. Part 1–Experimental Methods and Data Repositories.” <i>Cell Reports Physical Science</i>, vol. 7, no. 4, 103212, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.xcrp.2026.103212\">10.1016/j.xcrp.2026.103212</a>.","ieee":"S. Asche <i>et al.</i>, “What it takes to solve the origin of life: An integrated review. Part 1–Experimental methods and data repositories,” <i>Cell Reports Physical Science</i>, vol. 7, no. 4. Cell Press, 2026.","ista":"Asche S, Bautista C, Blanco C, Boulesteix D, Champagne-Ruel A, Mathis C, Markovitch O, Peng Z, Dass AV, Adams A, Camprubi E, Colizzi ES, Colón-Santos S, Dromiack H, Erastova V, Garcia A, Grimaud G, Halpern A, Harrison SA, Jordan SF, Jia TZ, Kahana A, Kolchinsky A, Moron-Garcia O, Mizuuchi R, Nan J, Orlova Y, Pearce BKD, Paschek K, Preiner M, Pinna S, Rodríguez-Román E, Schwander L, Sharma S, Smith HB, Vieira A, Xavier JC. 2026. What it takes to solve the origin of life: An integrated review. Part 1–Experimental methods and data repositories. Cell Reports Physical Science. 7(4), 103212.","short":"S. Asche, C. Bautista, C. Blanco, D. Boulesteix, A. Champagne-Ruel, C. Mathis, O. Markovitch, Z. Peng, A.V. Dass, A. Adams, E. Camprubi, E.S. Colizzi, S. Colón-Santos, H. Dromiack, V. Erastova, A. Garcia, G. Grimaud, A. Halpern, S.A. Harrison, S.F. Jordan, T.Z. Jia, A. Kahana, A. Kolchinsky, O. Moron-Garcia, R. Mizuuchi, J. Nan, Y. Orlova, B.K.D. Pearce, K. Paschek, M. Preiner, S. Pinna, E. Rodríguez-Román, L. Schwander, S. Sharma, H.B. Smith, A. Vieira, J.C. Xavier, Cell Reports Physical Science 7 (2026).","chicago":"Asche, Silke, Carla Bautista, Celia Blanco, David Boulesteix, Alexandre Champagne-Ruel, Cole Mathis, Omer Markovitch, et al. “What It Takes to Solve the Origin of Life: An Integrated Review. Part 1–Experimental Methods and Data Repositories.” <i>Cell Reports Physical Science</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.xcrp.2026.103212\">https://doi.org/10.1016/j.xcrp.2026.103212</a>.","apa":"Asche, S., Bautista, C., Blanco, C., Boulesteix, D., Champagne-Ruel, A., Mathis, C., … Xavier, J. C. (2026). What it takes to solve the origin of life: An integrated review. Part 1–Experimental methods and data repositories. <i>Cell Reports Physical Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.xcrp.2026.103212\">https://doi.org/10.1016/j.xcrp.2026.103212</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:59:19Z","_id":"21754","title":"What it takes to solve the origin of life: An integrated review. Part 1–Experimental methods and data repositories","date_created":"2026-04-19T22:07:52Z","status":"public","author":[{"full_name":"Asche, Silke","first_name":"Silke","last_name":"Asche"},{"first_name":"Carla","last_name":"Bautista","full_name":"Bautista, Carla"},{"full_name":"Blanco, Celia","first_name":"Celia","last_name":"Blanco"},{"full_name":"Boulesteix, David","last_name":"Boulesteix","first_name":"David"},{"full_name":"Champagne-Ruel, Alexandre","first_name":"Alexandre","last_name":"Champagne-Ruel"},{"last_name":"Mathis","first_name":"Cole","full_name":"Mathis, Cole"},{"full_name":"Markovitch, Omer","last_name":"Markovitch","first_name":"Omer"},{"full_name":"Peng, Zhen","first_name":"Zhen","last_name":"Peng"},{"full_name":"Dass, Avinash Vicholous","first_name":"Avinash Vicholous","last_name":"Dass"},{"full_name":"Adams, Alyssa","last_name":"Adams","first_name":"Alyssa"},{"full_name":"Camprubi, Eloi","last_name":"Camprubi","first_name":"Eloi"},{"last_name":"Colizzi","first_name":"Enrico Sandro","full_name":"Colizzi, Enrico Sandro"},{"last_name":"Colón-Santos","first_name":"Stephanie","full_name":"Colón-Santos, Stephanie"},{"full_name":"Dromiack, Hannah","last_name":"Dromiack","first_name":"Hannah"},{"first_name":"Valentina","last_name":"Erastova","full_name":"Erastova, Valentina"},{"full_name":"Garcia, Amanda","first_name":"Amanda","last_name":"Garcia"},{"full_name":"Grimaud, Ghjuvan","last_name":"Grimaud","first_name":"Ghjuvan"},{"full_name":"Halpern, Aaron","first_name":"Aaron","last_name":"Halpern"},{"full_name":"Harrison, Stuart A.","last_name":"Harrison","first_name":"Stuart A."},{"full_name":"Jordan, Seán F.","first_name":"Seán F.","last_name":"Jordan"},{"full_name":"Jia, Tony Z.","first_name":"Tony Z.","last_name":"Jia"},{"full_name":"Kahana, Amit","last_name":"Kahana","first_name":"Amit"},{"last_name":"Kolchinsky","first_name":"Artemy","full_name":"Kolchinsky, Artemy"},{"full_name":"Moron-Garcia, Odin","first_name":"Odin","last_name":"Moron-Garcia"},{"first_name":"Ryo","last_name":"Mizuuchi","full_name":"Mizuuchi, Ryo"},{"full_name":"Nan, Jingbo","first_name":"Jingbo","last_name":"Nan"},{"first_name":"Yuliia","last_name":"Orlova","full_name":"Orlova, Yuliia"},{"full_name":"Pearce, Ben K.D.","first_name":"Ben K.D.","last_name":"Pearce"},{"first_name":"Klaus","last_name":"Paschek","full_name":"Paschek, Klaus"},{"full_name":"Preiner, Martina","last_name":"Preiner","first_name":"Martina"},{"first_name":"Silvana","last_name":"Pinna","full_name":"Pinna, Silvana"},{"full_name":"Rodríguez-Román, Eduardo","last_name":"Rodríguez-Román","first_name":"Eduardo"},{"full_name":"Schwander, Loraine","first_name":"Loraine","last_name":"Schwander"},{"full_name":"Sharma, Siddhant","first_name":"Siddhant","last_name":"Sharma","id":"36996868-4916-11f1-8c9d-c0c901467b61"},{"last_name":"Smith","first_name":"Harrison B.","full_name":"Smith, Harrison B."},{"last_name":"Vieira","first_name":"Andrey","full_name":"Vieira, Andrey"},{"full_name":"Xavier, Joana C.","first_name":"Joana C.","last_name":"Xavier"}],"article_type":"original","oa_version":"Published Version","type":"journal_article","abstract":[{"lang":"eng","text":"The origin(s) of life (OoL), which has puzzled scientists for centuries, remains a major scientific challenge in the 21st century. Research on OoL spans many disciplines, including chemistry, physics, biology, planetary sciences, computer science, and mathematics. The sheer number of different scientific perspectives relevant to the problem has resulted in the coexistence of diverse tools, techniques, data, and software in OoL studies. This has made communication between the disciplines relevant to the OoL extremely difficult because the interpretation of data, analyses, or standards of evidence varies dramatically. Here, we hope to bridge this wide field of study by providing common ground via the consolidation of techniques rather than positing a unifying view on how life emerges. In part 1 of this review, we cover common experimental techniques that have been used significantly in OoL studies in recent years, while in part 2, we review theoretical, computational, and integrative methods. Here, we discuss the use of spectroscopy, spectrometry, chromatography, microscopy, and sequencing methods for characterizing diverse materials. We further discuss the role of data repositories in facilitating the analysis and dissemination of experimental data. This review provides a baseline expectation and understanding of the analytical aspects of origins’ research. Ultimately, we aim to provide an educational tool that can facilitate more post-disciplinary collaborations in OoL research by helping scientists understand what they can do about the problem of life’s origins, rather than telling them how to think about it."}],"article_number":"103212","volume":7,"file":[{"access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"checksum":"e580d22c2874c0afcbde2d167db7201b","date_created":"2026-05-07T05:48:23Z","file_name":"2026_CellREports_OoLEN1.pdf","content_type":"application/pdf","date_updated":"2026-05-07T05:48:23Z","file_size":3535247,"file_id":"21831"}],"doi":"10.1016/j.xcrp.2026.103212","day":"15","oa":1,"month":"04","publisher":"Cell Press","file_date_updated":"2026-05-07T05:48:23Z","date_published":"2026-04-15T00:00:00Z","has_accepted_license":"1","article_processing_charge":"Yes","fulldoi":"https://doi.org/10.1016/j.xcrp.2026.103212","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"ddc":["570"],"year":"2026","quality_controlled":"1","publication_status":"published","intvolume":"         7"},{"acknowledgement":"This work is a collaborative effort of the titled authors as part of the Origin of Life Early Career Network (OoLEN). We chose to add OoLEN as the first author to give a better representation of this team effort, rather than listing any single author as the first author. We hope such a thing can be adopted by others. We indicate that authors 2–9 (S.A., C.B., C. Blanco, D.B., A.C.-R., C.M., O.M., Z.P., and A.V.D.) have made a more distinct contribution. All authors are listed alphabetically by their last names. We would like to acknowledge all current and past members of OoLEN for their contributions to our community. In particular, we would like to acknowledge Evrim Fer, who helped with molecular phylogenetics. We would like to thank the anonymous referees for reviewing Parts 1 and 2 of this manuscript; this work was significantly improved through their feedback. S.A. acknowledges support from NASA through the postdoctoral Program at GSFC. C. Bautista acknowledges support from “la Caixa” Foundation (ID 100010434) under agreement (LCF/BQ/AA16/11580051) and by the Fonds de recherche du Québec Nature et technologies (FRQNT) (#274987). C. Blanco acknowledges support from NASA under award 80NSSC21K0595. D.B. acknowledges support from Centre national d'études spatiales (CNES) and postdoctoral support from LGPM-CentralSupélec and NASA under award 80NSSC23K1477. E. Camprubi acknowledges support from UT System for a STARs award. A.C.-R. acknowledges funding from the Natural Sciences and Engineering Research Council of Canada (grant number RGPIN/05278–2018), the Fonds de recherche Nature et Technologies of Québec (grant number 314488), and the Fondation J. Armand Bombardier Excellence Scholarship. A.C.-R.’s research was supported by an appointment to the NASA Postdoctoral Program from the NASA Astrobiology Program administered by Oak Ridge Associated Universities under contract with NASA. S.F.J. acknowledges support from “la Caixa” Foundation (ID 100010434) and from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska Curie grant agreement no. 847648 (the fellowship code is LCF/BQ/PI21/11830015). T.Z.J. acknowledges support from Japan Society for the Promotion of Science (JSPS) grants-in-aid 18K14354 and 21K14746, a Tokyo Institute of Technology Yoshinori Ohsumi Fund for Fundamental Research, the Mizuho Foundation for the Promotion of Sciences, and by the Temporary Assistant Program by the DE&I Section of Science Tokyo. A.K. acknowledges support from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant agreement no. 101068029. C.M. acknowledges support from NASA through the postdoctoral Fellowship Program. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of NASA. O.M. acknowledges support from The John Templeton Foundation (#62828) and the Foundation for Science and Technology (2023.05971.CEECIND). B.K.D.P. acknowledges support from the NSERC Banting Postdoctoral Fellowship. K.P. acknowledges financial support from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy EXC 2181/1 - 390900948 (the Heidelberg STRUCTURES Excellence Cluster) and is a fellow of the International Max Planck Research School for Astronomy and Cosmic Physics at the University of Heidelberg (IMPRS-HD).","publication_identifier":{"eissn":["2666-3864"]},"scopus_import":"1","issue":"4","publication":"Cell Reports Physical Science","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"gold","abstract":[{"text":"The origin(s) of life (OoL), which has puzzled scientists for centuries, remains a major scientific challenge in the 21st century. Understanding the processes relevant to the OoL demands theoretical frameworks that can connect processes across scales, from microscopic dynamics to emergent levels of organization. While experimental studies generate a wealth of data, theoretical and computational approaches provide the structure necessary to interpret and generalize these findings. In Part 1, we examined the most widely used experimental techniques in the field. Here, we focus on the mathematical, physical, and computational techniques used to model phenomena relevant to life’s origin(s). We discuss methods ranging from quantum chemistry and molecular dynamics to chemical reaction networks, autocatalysis, and evolutionary modeling, as well as information-theoretic and phylogenetic approaches that link chemical and biological organization. We further highlight emerging trends such as synthetic biology, omics-based methods, and laboratory automation as novel points of contact for theory-experiment integration. Ultimately, we aim to provide an educational tool that can facilitate more post-disciplinary collaborations in OoL research by helping scientists understand what they can do about the problem of life’s origins, rather than telling them how to think about it.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"original","author":[{"full_name":"Asche, Silke","last_name":"Asche","first_name":"Silke"},{"first_name":"Carla","last_name":"Bautista","full_name":"Bautista, Carla"},{"last_name":"Blanco","first_name":"Celia","full_name":"Blanco, Celia"},{"last_name":"Boulesteix","first_name":"David","full_name":"Boulesteix, David"},{"full_name":"Champagne-Ruel, Alexandre","last_name":"Champagne-Ruel","first_name":"Alexandre"},{"first_name":"Cole","last_name":"Mathis","full_name":"Mathis, Cole"},{"full_name":"Markovitch, Omer","last_name":"Markovitch","first_name":"Omer"},{"first_name":"Zhen","last_name":"Peng","full_name":"Peng, Zhen"},{"first_name":"Avinash Vicholous","last_name":"Dass","full_name":"Dass, Avinash Vicholous"},{"last_name":"Adams","first_name":"Alyssa","full_name":"Adams, Alyssa"},{"full_name":"Camprubi, Eloi","first_name":"Eloi","last_name":"Camprubi"},{"first_name":"Enrico Sandro","last_name":"Colizzi","full_name":"Colizzi, Enrico Sandro"},{"full_name":"Colón-Santos, Stephanie","first_name":"Stephanie","last_name":"Colón-Santos"},{"full_name":"Dromiack, Hannah","last_name":"Dromiack","first_name":"Hannah"},{"last_name":"Erastova","first_name":"Valentina","full_name":"Erastova, Valentina"},{"full_name":"Garcia, Amanda","last_name":"Garcia","first_name":"Amanda"},{"last_name":"Grimaud","first_name":"Ghjuvan","full_name":"Grimaud, Ghjuvan"},{"full_name":"Halpern, Aaron","first_name":"Aaron","last_name":"Halpern"},{"full_name":"Harrison, Stuart A.","first_name":"Stuart A.","last_name":"Harrison"},{"last_name":"Jordan","first_name":"Seán F.","full_name":"Jordan, Seán F."},{"last_name":"Jia","first_name":"Tony Z.","full_name":"Jia, Tony Z."},{"full_name":"Kahana, Amit","first_name":"Amit","last_name":"Kahana"},{"full_name":"Kolchinsky, Artemy","last_name":"Kolchinsky","first_name":"Artemy"},{"last_name":"Moron-Garcia","first_name":"Odin","full_name":"Moron-Garcia, Odin"},{"full_name":"Mizuuchi, Ryo","first_name":"Ryo","last_name":"Mizuuchi"},{"full_name":"Nan, Jingbo","last_name":"Nan","first_name":"Jingbo"},{"full_name":"Orlova, Yuliia","first_name":"Yuliia","last_name":"Orlova"},{"full_name":"Pearce, Ben K.D.","first_name":"Ben K.D.","last_name":"Pearce"},{"last_name":"Paschek","first_name":"Klaus","full_name":"Paschek, Klaus"},{"full_name":"Preiner, Martina","first_name":"Martina","last_name":"Preiner"},{"full_name":"Pinna, Silvana","first_name":"Silvana","last_name":"Pinna"},{"first_name":"Eduardo","last_name":"Rodríguez-Román","full_name":"Rodríguez-Román, Eduardo"},{"full_name":"Schwander, Loraine","first_name":"Loraine","last_name":"Schwander"},{"id":"36996868-4916-11f1-8c9d-c0c901467b61","first_name":"Siddhant","last_name":"Sharma","full_name":"Sharma, Siddhant"},{"full_name":"Smith, Harrison B.","first_name":"Harrison B.","last_name":"Smith"},{"full_name":"Vieira, Andrey","first_name":"Andrey","last_name":"Vieira"},{"full_name":"Xavier, Joana C.","first_name":"Joana C.","last_name":"Xavier"}],"status":"public","date_created":"2026-04-19T22:07:50Z","title":"What it takes to solve the origins of life: An integrated review. Part 2: Theoretical methods and emerging trends","_id":"21753","date_updated":"2026-10-20T08:58:31Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","DOAJ_listed":"1","citation":{"short":"S. Asche, C. Bautista, C. Blanco, D. Boulesteix, A. Champagne-Ruel, C. Mathis, O. Markovitch, Z. Peng, A.V. Dass, A. Adams, E. Camprubi, E.S. Colizzi, S. Colón-Santos, H. Dromiack, V. Erastova, A. Garcia, G. Grimaud, A. Halpern, S.A. Harrison, S.F. Jordan, T.Z. Jia, A. Kahana, A. Kolchinsky, O. Moron-Garcia, R. Mizuuchi, J. Nan, Y. Orlova, B.K.D. Pearce, K. Paschek, M. Preiner, S. Pinna, E. Rodríguez-Román, L. Schwander, S. Sharma, H.B. Smith, A. Vieira, J.C. Xavier, Cell Reports Physical Science 7 (2026).","ista":"Asche S, Bautista C, Blanco C, Boulesteix D, Champagne-Ruel A, Mathis C, Markovitch O, Peng Z, Dass AV, Adams A, Camprubi E, Colizzi ES, Colón-Santos S, Dromiack H, Erastova V, Garcia A, Grimaud G, Halpern A, Harrison SA, Jordan SF, Jia TZ, Kahana A, Kolchinsky A, Moron-Garcia O, Mizuuchi R, Nan J, Orlova Y, Pearce BKD, Paschek K, Preiner M, Pinna S, Rodríguez-Román E, Schwander L, Sharma S, Smith HB, Vieira A, Xavier JC. 2026. What it takes to solve the origins of life: An integrated review. Part 2: Theoretical methods and emerging trends. Cell Reports Physical Science. 7(4), 103211.","ieee":"S. Asche <i>et al.</i>, “What it takes to solve the origins of life: An integrated review. Part 2: Theoretical methods and emerging trends,” <i>Cell Reports Physical Science</i>, vol. 7, no. 4. Cell Press, 2026.","mla":"Asche, Silke, et al. “What It Takes to Solve the Origins of Life: An Integrated Review. Part 2: Theoretical Methods and Emerging Trends.” <i>Cell Reports Physical Science</i>, vol. 7, no. 4, 103211, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.xcrp.2026.103211\">10.1016/j.xcrp.2026.103211</a>.","ama":"Asche S, Bautista C, Blanco C, et al. What it takes to solve the origins of life: An integrated review. Part 2: Theoretical methods and emerging trends. <i>Cell Reports Physical Science</i>. 2026;7(4). doi:<a href=\"https://doi.org/10.1016/j.xcrp.2026.103211\">10.1016/j.xcrp.2026.103211</a>","apa":"Asche, S., Bautista, C., Blanco, C., Boulesteix, D., Champagne-Ruel, A., Mathis, C., … Xavier, J. C. (2026). What it takes to solve the origins of life: An integrated review. Part 2: Theoretical methods and emerging trends. <i>Cell Reports Physical Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.xcrp.2026.103211\">https://doi.org/10.1016/j.xcrp.2026.103211</a>","chicago":"Asche, Silke, Carla Bautista, Celia Blanco, David Boulesteix, Alexandre Champagne-Ruel, Cole Mathis, Omer Markovitch, et al. “What It Takes to Solve the Origins of Life: An Integrated Review. Part 2: Theoretical Methods and Emerging Trends.” <i>Cell Reports Physical Science</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.xcrp.2026.103211\">https://doi.org/10.1016/j.xcrp.2026.103211</a>."},"publisher":"Cell Press","month":"04","oa":1,"day":"15","doi":"10.1016/j.xcrp.2026.103211","file":[{"content_type":"application/pdf","date_updated":"2026-05-06T06:48:33Z","file_name":"2026_CellReports_OoLEN.pdf","file_size":5265320,"file_id":"21804","access_level":"open_access","creator":"dernst","relation":"main_file","success":1,"checksum":"d5abe5b5bd4b9ee58aa6f4917f688ad9","date_created":"2026-05-06T06:48:33Z"}],"volume":7,"article_number":"103211","intvolume":"         7","publication_status":"published","year":"2026","quality_controlled":"1","ddc":["570"],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"fulldoi":"https://doi.org/10.1016/j.xcrp.2026.103211","article_processing_charge":"Yes","has_accepted_license":"1","file_date_updated":"2026-05-06T06:48:33Z","date_published":"2026-04-15T00:00:00Z"},{"date_updated":"2026-10-20T08:58:10Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"Y. Li <i>et al.</i>, “A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types,” <i>Cell Reports</i>, vol. 45, no. 2. Cell Press, 2026.","ista":"Li Y, Butler TC, Nardone S, Jacobs CL, Douglass AM, Madara JC, McDonough MC, Tao J, Lowenstein ED, Wang L, Pant D, Walker SJ, Wang A, Srinivasan H, Yang Z, Campbell JN, Tsai LT, Lowell BB, Resch JM. 2026. A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. Cell Reports. 45(2), 116904.","short":"Y. Li, T.C. Butler, S. Nardone, C.L. Jacobs, A.M. Douglass, J.C. Madara, M.C. McDonough, J. Tao, E.D. Lowenstein, L. Wang, D. Pant, S.J. Walker, A. Wang, H. Srinivasan, Z. Yang, J.N. Campbell, L.T. Tsai, B.B. Lowell, J.M. Resch, Cell Reports 45 (2026).","ama":"Li Y, Butler TC, Nardone S, et al. A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. 2026;45(2). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">10.1016/j.celrep.2025.116904</a>","mla":"Li, Yuxi, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>, vol. 45, no. 2, 116904, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">10.1016/j.celrep.2025.116904</a>.","apa":"Li, Y., Butler, T. C., Nardone, S., Jacobs, C. L., Douglass, A. M., Madara, J. C., … Resch, J. M. (2026). A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">https://doi.org/10.1016/j.celrep.2025.116904</a>","chicago":"Li, Yuxi, Trevor C. Butler, Stefano Nardone, Christopher L. Jacobs, Amelia M. Douglass, Joseph C. Madara, Miriam C. McDonough, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">https://doi.org/10.1016/j.celrep.2025.116904</a>."},"DOAJ_listed":"1","abstract":[{"text":"The paraventricular hypothalamus (PVH) controls behavioral and physiologic processes, including appetite, social behavior, autonomic outflow, and pituitary hormone secretion. However, molecular markers for centrally projecting PVH neuron populations remain largely undefined, and a complete census of PVH cell types has not been established. Therefore, we performed extensive single-cell/nucleus RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic atlas resolves 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding areas. Additionally, projection-based profiling identified neurons that project to the parabrachial region (PB) and spinal cord, helping to determine PVH populations that regulate satiety and sympathetic nervous system activity, respectively. Notably, activation of PB-projecting PVH neurons expressing Brs3 reduces food intake, and silencing them causes obesity. Together, this atlas contributes high-resolution PVH spatial and circuit-based gene expression profiles, representing a valuable resource for the field of homeostasis.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"original","author":[{"last_name":"Li","first_name":"Yuxi","full_name":"Li, Yuxi"},{"full_name":"Butler, Trevor C.","first_name":"Trevor C.","last_name":"Butler"},{"full_name":"Nardone, Stefano","last_name":"Nardone","first_name":"Stefano"},{"first_name":"Christopher L.","last_name":"Jacobs","full_name":"Jacobs, Christopher L."},{"last_name":"Douglass","first_name":"Amelia May Barnett","id":"de5f6fda-80fb-11ef-996f-a8c4ecd8e289","orcid":"0000-0001-5398-6473","full_name":"Douglass, Amelia May Barnett"},{"first_name":"Joseph C.","last_name":"Madara","full_name":"Madara, Joseph C."},{"last_name":"McDonough","first_name":"Miriam C.","full_name":"McDonough, Miriam C."},{"full_name":"Tao, Jenkang","last_name":"Tao","first_name":"Jenkang"},{"full_name":"Lowenstein, Elijah D.","last_name":"Lowenstein","first_name":"Elijah D."},{"full_name":"Wang, Luhong","last_name":"Wang","first_name":"Luhong"},{"last_name":"Pant","first_name":"Deepti","full_name":"Pant, Deepti"},{"full_name":"Walker, Samuel J.","first_name":"Samuel J.","last_name":"Walker"},{"first_name":"Annette","last_name":"Wang","full_name":"Wang, Annette"},{"last_name":"Srinivasan","first_name":"Harini","full_name":"Srinivasan, Harini"},{"full_name":"Yang, Zongfang","last_name":"Yang","first_name":"Zongfang"},{"full_name":"Campbell, John N.","last_name":"Campbell","first_name":"John N."},{"full_name":"Tsai, Linus T.","first_name":"Linus T.","last_name":"Tsai"},{"full_name":"Lowell, Bradford B.","last_name":"Lowell","first_name":"Bradford B."},{"full_name":"Resch, Jon M.","last_name":"Resch","first_name":"Jon M."}],"date_created":"2026-04-16T13:51:29Z","status":"public","title":"A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types","_id":"21744","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"gold","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"acknowledgement":"We would like to thank Drs. Mark Andermann, Joel Geerling, and Clifford\r\nSaper, as well as the Lowell, Tsai, and Resch laboratories for helpful discussions;\r\nAlysia Berns, Jia Yu, and Yanfang Li for technical support; the BNORC\r\nFunctional Genomics and Bioinformatics Core (P30DK046200) and the Iowa\r\nInstitute for Human Genetics Genomics Division (IIHG, RRID: SCR_023422)\r\nfor helpful discussions and technical assistance with sc/snRNA-seq; Zachary\r\nNiziolek and the Bauer Core Facility at Harvard University, the BIDMC Flow Cytometry\r\nCore, and Heath Vignes, Michael Shey, and Thomas Kaufman of the\r\nFlow Cytometry Facility at the University of Iowa Carver College of Medicine\r\nfor helpful discussions and technical support; the ICCB-Longwood Screening\r\nFacility of Harvard Medical School for assistance with the snRNA-seq\r\nexperiments; Dr. Sayak Mitter and Vizgen support for technical assistance\r\nwith the MERSCOPE platform; and Mara Jendro and Li-Chun (Queena) Lin\r\nfor their assistance with MERSCOPE experiments within the Iowa\r\nNeuroBank Core in the Iowa Neuroscience Institute at the University of Iowa\r\nCarver College of Medicine. This research was funded by the following NIH\r\ngrants to L.T.T.: R01DK128406; to B.B.L.: R01DK075632, R01DK134427,\r\nand R01DK096010; to J.M.R.: R00HL144923 and R01NS141072; and to M.C.M.: F31HL170784; T.C.B. and M.C.M. were supported by a pharmacological\r\nsciences predoctoral training grant T32GM144636. Additional funding\r\nto J.M.R. came from the American Heart Association (AHA 935362), a University\r\nof Iowa Fraternal Order of Eagles Diabetes Research Center Pilot and\r\nFeasibility Catalyst Grant, and an Iowa Neuroscience Institute Early Stage\r\nInvestigator award from the Carver Trust. Y.L. was supported by a predoctoral\r\nfellowship from the American Heart Association (AHA 25PRE1372983). A.M.D.\r\nwas supported by a postdoctoral fellowship from the Charles A. King Trust.","scopus_import":"1","issue":"2","publication":"Cell Reports","fulldoi":"https://doi.org/10.1016/j.celrep.2025.116904","article_processing_charge":"Yes","has_accepted_license":"1","file_date_updated":"2026-05-04T11:58:51Z","date_published":"2026-02-24T00:00:00Z","intvolume":"        45","publication_status":"published","pmid":1,"year":"2026","quality_controlled":"1","ddc":["570"],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"doi":"10.1016/j.celrep.2025.116904","file":[{"file_id":"21793","file_name":"2026_CellReports_Li.pdf","date_updated":"2026-05-04T11:58:51Z","content_type":"application/pdf","file_size":38532865,"success":1,"checksum":"82098dd9d0ca609119f9f2c6beb4fc1e","date_created":"2026-05-04T11:58:51Z","access_level":"open_access","creator":"dernst","relation":"main_file"}],"volume":45,"article_number":"116904","publisher":"Cell Press","department":[{"_id":"AmDo"}],"month":"02","external_id":{"pmid":["41581146"]},"oa":1,"day":"24"},{"language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"hybrid","acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","publication_identifier":{"issn":["0960-9822"]},"issue":"6","publication":"Current Biology","PlanS_conform":"1","date_updated":"2026-10-20T08:57:40Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"1468-1480.e6","citation":{"apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., &#38; Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>.","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” <i>Current Biology</i>, vol. 36, no. 6. Cell Press, p. 1468–1480.e6, 2026.","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6.","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. 2026;36(6):1468-1480.e6. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>, vol. 36, no. 6, Cell Press, 2026, p. 1468–1480.e6, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>."},"project":[{"grant_number":"101142681","name":"Cyclic nucleotides as second messengers in plants","_id":"8f347782-16d5-11f0-9cad-8c19706ee739"},{"_id":"bd906599-d553-11ed-ba76-abf8547645d7","name":"Identification of a novel regulator in auxin canalization","grant_number":"E271"}],"abstract":[{"lang":"eng","text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development."}],"oa_version":"Published Version","type":"journal_article","article_type":"original","status":"public","author":[{"full_name":"Li, Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","first_name":"Mingyue","last_name":"Li"},{"full_name":"Rydza, Nikola","last_name":"Rydza","first_name":"Nikola"},{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar","first_name":"Gergely","full_name":"Molnar, Gergely"},{"full_name":"Nodzyński, Tomasz","last_name":"Nodzyński","first_name":"Tomasz"},{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří"}],"date_created":"2026-03-23T15:11:16Z","title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","_id":"21490","doi":"10.1016/j.cub.2026.02.023","file":[{"relation":"main_file","creator":"dernst","access_level":"open_access","checksum":"fe6c41fdab58a55df5f2a5860c02acdc","date_created":"2026-03-24T08:34:37Z","success":1,"file_size":12986894,"file_name":"2026_CurrentBiology_Li.pdf","date_updated":"2026-03-24T08:34:37Z","content_type":"application/pdf","file_id":"21496"}],"volume":36,"publisher":"Cell Press","department":[{"_id":"JiFr"}],"month":"03","external_id":{"pmid":["41831441"]},"oa":1,"corr_author":"1","day":"23","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"fulldoi":"https://doi.org/10.1016/j.cub.2026.02.023","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","file_date_updated":"2026-03-24T08:34:37Z","date_published":"2026-03-23T00:00:00Z","intvolume":"        36","publication_status":"published","year":"2026","pmid":1,"quality_controlled":"1","ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"intvolume":"        86","year":"2026","pmid":1,"quality_controlled":"1","publication_status":"published","article_processing_charge":"Yes (in subscription journal)","file_date_updated":"2026-03-30T12:04:38Z","date_published":"2026-02-19T00:00:00Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.molcel.2026.01.021","oa":1,"day":"19","month":"02","department":[{"_id":"AlMi"}],"publisher":"Cell Press","external_id":{"pmid":["41679301"]},"file":[{"file_id":"21510","file_size":9786677,"file_name":"2026_MolecularCell_Weiss.pdf","date_updated":"2026-03-30T12:04:38Z","content_type":"application/pdf","date_created":"2026-03-30T12:04:38Z","checksum":"e16a7315b64a706184b177ea1621523c","success":1,"creator":"dernst","relation":"main_file","access_level":"open_access"}],"doi":"10.1016/j.molcel.2026.01.021","volume":86,"status":"public","date_created":"2026-03-30T11:58:48Z","author":[{"full_name":"Weiss, Joscha","last_name":"Weiss","first_name":"Joscha"},{"last_name":"Vecchia","first_name":"Luca","full_name":"Vecchia, Luca"},{"full_name":"Domjan, David","last_name":"Domjan","first_name":"David"},{"full_name":"Cavadini, Simone","first_name":"Simone","last_name":"Cavadini"},{"full_name":"Sabantsev, Anton","last_name":"Sabantsev","first_name":"Anton"},{"last_name":"Kempf","first_name":"Georg","full_name":"Kempf, Georg"},{"full_name":"Pathare, Ganesh R.","first_name":"Ganesh R.","last_name":"Pathare"},{"first_name":"Klaus","last_name":"Brackmann","full_name":"Brackmann, Klaus"},{"id":"6437c950-2a03-11ee-914d-d6476dd7b75c","last_name":"Michael","first_name":"Alicia","full_name":"Michael, Alicia","orcid":"0000-0002-6080-839X"},{"last_name":"Kater","first_name":"Lukas","full_name":"Kater, Lukas"},{"first_name":"Eric","last_name":"Hietter-Pfeiffer","full_name":"Hietter-Pfeiffer, Eric"},{"full_name":"Haddawi, Mina","first_name":"Mina","last_name":"Haddawi"},{"last_name":"Kuber","first_name":"Urja P.","full_name":"Kuber, Urja P."},{"last_name":"Mühlhäusser","first_name":"Sandra","full_name":"Mühlhäusser, Sandra"},{"full_name":"Grand, Ralph S.","first_name":"Ralph S.","last_name":"Grand"},{"full_name":"Stadler, Michael B.","last_name":"Stadler","first_name":"Michael B."},{"full_name":"Deindl, Sebastian","first_name":"Sebastian","last_name":"Deindl"},{"last_name":"Thomä","first_name":"Nicolas H.","full_name":"Thomä, Nicolas H."}],"_id":"21509","title":"The human BAF chromatin remodeler processes nucleosomes bound by pioneer transcription factors OCT4–SOX2","abstract":[{"lang":"eng","text":"Chromatin remodeling complexes mobilize nucleosomes and promote transcription factor (TF) binding. Using ensemble and single-molecule assays combined with cryo-electron microscopy (cryo-EM), we studied the interaction between pioneer TFs OCT4–SOX2 and the human BRG1/BRM-associated factor (BAF) complex on nucleosomes. BAF engages TF-bound substrates in two orientations, placing OCT4–SOX2 at either the remodeler ENTRY or EXIT site. At the ENTRY site, OCT4–SOX2 initially coexists with BAF without structural interference. However, continued DNA translocation is expected to cause collisions with bound TFs, which can trigger remodeling direction reversals or may induce TF dissociation. To accommodate TFs at the EXIT site, BAF undergoes structural rearrangements, and ensemble assays reveal a nucleosome subpopulation translocating away from TF-binding sites. Moreover, single-molecule experiments show that nucleosome-bound BAF frequently changes remodeling direction, and we identify an ADP-bound remodeler conformation as a potential intermediate. Together, these findings reveal key aspects of the conformational dynamics and remodeling outcomes underlying BAF processing of TF-bound nucleosomes."}],"article_type":"original","oa_version":"Published Version","type":"journal_article","citation":{"ieee":"J. Weiss <i>et al.</i>, “The human BAF chromatin remodeler processes nucleosomes bound by pioneer transcription factors OCT4–SOX2,” <i>Molecular Cell</i>, vol. 86, no. 4. Cell Press, p. 625–639.e8, 2026.","ista":"Weiss J, Vecchia L, Domjan D, Cavadini S, Sabantsev A, Kempf G, Pathare GR, Brackmann K, Michael AK, Kater L, Hietter-Pfeiffer E, Haddawi M, Kuber UP, Mühlhäusser S, Grand RS, Stadler MB, Deindl S, Thomä NH. 2026. The human BAF chromatin remodeler processes nucleosomes bound by pioneer transcription factors OCT4–SOX2. Molecular Cell. 86(4), 625–639.e8.","short":"J. Weiss, L. Vecchia, D. Domjan, S. Cavadini, A. Sabantsev, G. Kempf, G.R. Pathare, K. Brackmann, A.K. Michael, L. Kater, E. Hietter-Pfeiffer, M. Haddawi, U.P. Kuber, S. Mühlhäusser, R.S. Grand, M.B. Stadler, S. Deindl, N.H. Thomä, Molecular Cell 86 (2026) 625–639.e8.","mla":"Weiss, Joscha, et al. “The Human BAF Chromatin Remodeler Processes Nucleosomes Bound by Pioneer Transcription Factors OCT4–SOX2.” <i>Molecular Cell</i>, vol. 86, no. 4, Cell Press, 2026, p. 625–639.e8, doi:<a href=\"https://doi.org/10.1016/j.molcel.2026.01.021\">10.1016/j.molcel.2026.01.021</a>.","ama":"Weiss J, Vecchia L, Domjan D, et al. The human BAF chromatin remodeler processes nucleosomes bound by pioneer transcription factors OCT4–SOX2. <i>Molecular Cell</i>. 2026;86(4):625-639.e8. doi:<a href=\"https://doi.org/10.1016/j.molcel.2026.01.021\">10.1016/j.molcel.2026.01.021</a>","apa":"Weiss, J., Vecchia, L., Domjan, D., Cavadini, S., Sabantsev, A., Kempf, G., … Thomä, N. H. (2026). The human BAF chromatin remodeler processes nucleosomes bound by pioneer transcription factors OCT4–SOX2. <i>Molecular Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molcel.2026.01.021\">https://doi.org/10.1016/j.molcel.2026.01.021</a>","chicago":"Weiss, Joscha, Luca Vecchia, David Domjan, Simone Cavadini, Anton Sabantsev, Georg Kempf, Ganesh R. Pathare, et al. “The Human BAF Chromatin Remodeler Processes Nucleosomes Bound by Pioneer Transcription Factors OCT4–SOX2.” <i>Molecular Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.molcel.2026.01.021\">https://doi.org/10.1016/j.molcel.2026.01.021</a>."},"page":"625-639.e8","PlanS_conform":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:57:55Z","issue":"4","scopus_import":"1","publication":"Molecular Cell","publication_identifier":{"issn":["1097-2765"]},"acknowledgement":"We thank D. Hess, V. Iesmantavicius, and J. Seebacher (FMI Proteomics and Protein Analysis Facility) for mass spectrometry support; S. Smallwood, K. Shimada, D. Klein, and M. Schütz-Stoffregen for technical assistance; J. Côté and C. Lachance for critical discussions; and members of the Thomä lab for helpful feedback. Support for this work was provided to N.H.T. by the European Research Council under the European Union’s Horizon 2020 research program (NucEM, no. 884331), the Novartis Research Foundation, the Swiss National Science Foundation (SNF 31003A_179541, 310030_214852, and Sinergia CRSII5_186230), and the Swiss Cancer Research (KFS-4980-02-2020 and KFS-5933-08-2023). S.D. was supported by the European Research Council (DONUTS, no. 101092623), the Knut and Alice Wallenberg Foundation (2024.0012), the Cancerfonden (25 4453 Pj), and the Swedish Research Council (VR 03255). A.K.M. was supported by a Human Frontier Science Program Long-Term Fellowship, and L.V. was supported by an EMBO fellowship (ALTF 549-2021).","OA_type":"hybrid","OA_place":"publisher","language":[{"iso":"eng"}]},{"author":[{"full_name":"Perez Verdugo, Fernanda L","last_name":"Perez Verdugo","first_name":"Fernanda L","id":"4ecec223-9070-11ef-a0a9-bc76077bea8d"},{"first_name":"Eirini","last_name":"Maniou","full_name":"Maniou, Eirini"},{"full_name":"Galea, Gabriel L.","first_name":"Gabriel L.","last_name":"Galea"},{"first_name":"Shiladitya","last_name":"Banerjee","full_name":"Banerjee, Shiladitya"}],"status":"public","date_created":"2026-04-26T22:01:46Z","_id":"21761","title":"Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis","abstract":[{"text":"Neural tube closure is a critical morphogenetic process in vertebrate development, and failure to close cranial regions such as the hindbrain neuropore (HNP) leads to severe congenital malformations. While mechanical forces such as actomyosin purse-string contraction and directional cell crawling have been implicated in driving HNP closure, how these forces organize local cell shape and motion to produce large-scale tissue remodeling remains poorly understood. Using live and fixed imaging of mouse embryos combined with cell-based biophysical modeling, we show that these force-generating mechanisms are insufficient to explain the reproducible patterns of cell elongation and nematic alignment observed at the HNP border. Instead, we show that local anisotropic stress and cytoskeletal organization are required to generate these patterns and promote midline cell motion. Our model captures key features of cell shape dynamics and emergent nematic order, which we confirm experimentally, including the alignment of actin fibers with cell shape and enhanced midline cell speed. Comparative analysis with chick embryos, which lack supracellular purse strings, supports a conserved link between tension generation and cellular patterning. These findings establish a physical framework connecting force generation, cell shape anisotropy, and tissue morphodynamics during epithelial gap closure.","lang":"eng"}],"article_type":"original","type":"journal_article","oa_version":"Published Version","page":"1903-1917.e5","citation":{"apa":"Perez Verdugo, F. L., Maniou, E., Galea, G. L., &#38; Banerjee, S. (2026). Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">https://doi.org/10.1016/j.cub.2026.02.068</a>","chicago":"Perez Verdugo, Fernanda L, Eirini Maniou, Gabriel L. Galea, and Shiladitya Banerjee. “Mechanosensitive Feedback Organizes Cell Shape and Motion during Hindbrain Neuropore Morphogenesis.” <i>Current Biology</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">https://doi.org/10.1016/j.cub.2026.02.068</a>.","ista":"Perez Verdugo FL, Maniou E, Galea GL, Banerjee S. 2026. Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis. Current Biology. 36(8), 1903–1917.e5.","short":"F.L. Perez Verdugo, E. Maniou, G.L. Galea, S. Banerjee, Current Biology 36 (2026) 1903–1917.e5.","ieee":"F. L. Perez Verdugo, E. Maniou, G. L. Galea, and S. Banerjee, “Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis,” <i>Current Biology</i>, vol. 36, no. 8. Cell Press, p. 1903–1917.e5, 2026.","ama":"Perez Verdugo FL, Maniou E, Galea GL, Banerjee S. Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis. <i>Current Biology</i>. 2026;36(8):1903-1917.e5. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">10.1016/j.cub.2026.02.068</a>","mla":"Perez Verdugo, Fernanda L., et al. “Mechanosensitive Feedback Organizes Cell Shape and Motion during Hindbrain Neuropore Morphogenesis.” <i>Current Biology</i>, vol. 36, no. 8, Cell Press, 2026, p. 1903–1917.e5, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.068\">10.1016/j.cub.2026.02.068</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:59:48Z","issue":"8","scopus_import":"1","publication":"Current Biology","publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"acknowledgement":"S.B. acknowledges support from the National Institutes of Health (NIH R35 GM143042) and the National Science Foundation (NSF MCB-2203601). G.L.G. acknowledges support from the Wellcome Trust (211112/Z/18/Z), the Royal Society (RG\\R2\\232082), and the Leverhulme Trust (RPG-2024-147). E.M. acknowledges support from European Union’s Horizon 2021 Marie Sklodowska-Curie grant agreement no. 101067028. F.P.-V. acknowledges support from the NOMIS foundation. The surface subtraction macro is courtesy of Dr. Dale Moulding and available on GitHub (https://github.com/DaleMoulding/Fiji-Macros).","OA_place":"publisher","OA_type":"hybrid","language":[{"iso":"eng"}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"ddc":["570"],"intvolume":"        36","quality_controlled":"1","pmid":1,"year":"2026","publication_status":"published","article_processing_charge":"Yes (in subscription journal)","file_date_updated":"2026-04-28T13:13:40Z","date_published":"2026-04-20T00:00:00Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.cub.2026.02.068","oa":1,"day":"20","department":[{"_id":"AnSa"}],"month":"04","publisher":"Cell Press","external_id":{"pmid":["41881011"]},"file":[{"date_updated":"2026-04-28T13:13:40Z","content_type":"application/pdf","file_name":"2026_CurrentBiology_PerezVerdugo.pdf","file_size":13402043,"file_id":"21774","access_level":"open_access","creator":"dernst","relation":"main_file","success":1,"checksum":"80ae45457b4682c50c84f54de15aa9a8","date_created":"2026-04-28T13:13:40Z"}],"doi":"10.1016/j.cub.2026.02.068","volume":36},{"pmid":1,"quality_controlled":"1","year":"2026","publication_status":"inpress","fulldoi":"https://doi.org/10.1016/j.neuron.2026.05.010","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.09.27.678865"}],"date_published":"2026-06-03T00:00:00Z","keyword":["hunger","hypothalamus","AGRP neurons","neuroscience","metabolism","homeostasis","feeding","food intake","energy balance","appetite"],"article_processing_charge":"No","external_id":{"pmid":["42235510"]},"month":"06","department":[{"_id":"AmDo"}],"publisher":"Cell Press","day":"03","oa":1,"doi":"10.1016/j.neuron.2026.05.010","article_type":"original","oa_version":"Preprint","type":"journal_article","abstract":[{"lang":"eng","text":"AgRP neurons cause hunger, the drive to seek and consume food. Their activation by fasting is key for survival and is thought to be triggered by feedback when energy stores are low. However, we know that environmental cues can also regulate AgRP neurons since cues that predict future food intake rapidly inhibit AgRP neurons, but is the converse true: can the prediction of future fasting rapidly activate AgRP neurons? Here, we show in mice that such rapid fasting activation of AgRP neurons does occur. This rapid activation is driven by excitatory input from paraventricular hypothalamic (PVH) neurons expressing Sim2, which are bidirectionally sensitive to predictions of future energy state. Thus, cognitively processed contextual information conveyed by PVHSim2 neurons strongly activates AgRP neurons. Lastly, chronic silencing of PVHSim2 neurons causes persistent hypophagia. This PVHSim2-to-AgRP-neuron circuit, by anticipating and preventing negative energy balance, provides an important new dimension of hunger regulation."}],"_id":"21955","title":"A hypothalamic circuit for anticipating future changes in energy balance","date_created":"2026-06-08T09:24:25Z","author":[{"last_name":"Walker","first_name":"Samuel J.","full_name":"Walker, Samuel J."},{"full_name":"Lowenstein, Elijah D.","first_name":"Elijah D.","last_name":"Lowenstein"},{"full_name":"Douglass, Amelia May Barnett","orcid":"0000-0001-5398-6473","id":"de5f6fda-80fb-11ef-996f-a8c4ecd8e289","last_name":"Douglass","first_name":"Amelia May Barnett"},{"full_name":"Thomas, Callum M.P.","last_name":"Thomas","first_name":"Callum M.P."},{"last_name":"Madara","first_name":"Joseph C.","full_name":"Madara, Joseph C."},{"last_name":"Kucukdereli","first_name":"Hakan","full_name":"Kucukdereli, Hakan"},{"full_name":"Barbosa-Meillon, Eunice A.","last_name":"Barbosa-Meillon","first_name":"Eunice A."},{"full_name":"Tao, Jenkang","last_name":"Tao","first_name":"Jenkang"},{"full_name":"Resch, Jon M.","first_name":"Jon M.","last_name":"Resch"},{"full_name":"Lowell, Bradford B.","first_name":"Bradford B.","last_name":"Lowell"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T09:00:02Z","citation":{"ieee":"S. J. Walker <i>et al.</i>, “A hypothalamic circuit for anticipating future changes in energy balance,” <i>Neuron</i>. Cell Press.","ista":"Walker SJ, Lowenstein ED, Douglass AM, Thomas CMP, Madara JC, Kucukdereli H, Barbosa-Meillon EA, Tao J, Resch JM, Lowell BB. A hypothalamic circuit for anticipating future changes in energy balance. Neuron.","short":"S.J. Walker, E.D. Lowenstein, A.M. Douglass, C.M.P. Thomas, J.C. Madara, H. Kucukdereli, E.A. Barbosa-Meillon, J. Tao, J.M. Resch, B.B. Lowell, Neuron (n.d.).","mla":"Walker, Samuel J., et al. “A Hypothalamic Circuit for Anticipating Future Changes in Energy Balance.” <i>Neuron</i>, Cell Press, doi:<a href=\"https://doi.org/10.1016/j.neuron.2026.05.010\">10.1016/j.neuron.2026.05.010</a>.","ama":"Walker SJ, Lowenstein ED, Douglass AM, et al. A hypothalamic circuit for anticipating future changes in energy balance. <i>Neuron</i>. doi:<a href=\"https://doi.org/10.1016/j.neuron.2026.05.010\">10.1016/j.neuron.2026.05.010</a>","apa":"Walker, S. J., Lowenstein, E. D., Douglass, A. M., Thomas, C. M. P., Madara, J. C., Kucukdereli, H., … Lowell, B. B. (n.d.). A hypothalamic circuit for anticipating future changes in energy balance. <i>Neuron</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.neuron.2026.05.010\">https://doi.org/10.1016/j.neuron.2026.05.010</a>","chicago":"Walker, Samuel J., Elijah D. Lowenstein, Amelia M. Douglass, Callum M.P. Thomas, Joseph C. Madara, Hakan Kucukdereli, Eunice A. Barbosa-Meillon, Jenkang Tao, Jon M. Resch, and Bradford B. Lowell. “A Hypothalamic Circuit for Anticipating Future Changes in Energy Balance.” <i>Neuron</i>. Cell Press, n.d. <a href=\"https://doi.org/10.1016/j.neuron.2026.05.010\">https://doi.org/10.1016/j.neuron.2026.05.010</a>."},"publication_identifier":{"issn":["0896-6273"],"eissn":[" 1097-4199"]},"acknowledgement":"We thank all members of the B.B.L. laboratory for helpful discussions. We\r\nthank the BADERC and BNORC transgenic cores (NIH P30DK057521 and\r\nP30DK046200) for performing embryo injections to generate knockin mouse\r\nlines. We also thank the BIDMC Energy Balance Core (supported by NIH\r\nS10OD028635 and the Boston Area Diabetes Endocrinology Research Centers, P30DK135043), where Marissa Cortopassi performed indirect calorimetry experiments and Alexander Banks assisted with data analysis and interpretation. Confocal imaging was performed at BIDMC’s Confocal Imaging\r\nCore. We thank Chen Wu for assistance in designing knockin mouse lines.\r\nThis work was supported by the NIH (R01DK134427, R01DK096010, and\r\nR01DK075632 to B.B.L.). Authors were supported by an EMBO Long-Term\r\nFellowship (770-2018, S.J.W.), a T32 Postdoctoral Training Fellowship\r\n(5T32DK007516, E.D.L.), the Charles A. King Trust Postdoctoral Research\r\nFellowship program (A.M.D.), and a K99 Career Development Award\r\n(K99HL144923, J.M.R.).","publication":"Neuron","scopus_import":"1","language":[{"iso":"eng"}],"OA_type":"green","OA_place":"repository"},{"article_processing_charge":"No","date_published":"2026-06-25T00:00:00Z","fulldoi":"https://doi.org/10.1016/j.cell.2026.05.038","das_tickbox":"0","intvolume":"       189","year":"2026","quality_controlled":"1","publication_status":"published","researchdata_availability":"no","doi":"10.1016/j.cell.2026.05.038","volume":189,"corr_author":"1","day":"25","department":[{"_id":"MiSi"}],"month":"06","publisher":"Cell Press","page":"3845-3846","citation":{"apa":"Riedl, M., &#38; Sixt, M. K. (2026). A new sense for electrical fields. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">https://doi.org/10.1016/j.cell.2026.05.038</a>","chicago":"Riedl, Michael, and Michael K Sixt. “A New Sense for Electrical Fields.” <i>Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">https://doi.org/10.1016/j.cell.2026.05.038</a>.","ista":"Riedl M, Sixt MK. 2026. A new sense for electrical fields. Cell. 189(13), 3845–3846.","short":"M. Riedl, M.K. Sixt, Cell 189 (2026) 3845–3846.","ieee":"M. Riedl and M. K. Sixt, “A new sense for electrical fields,” <i>Cell</i>, vol. 189, no. 13. Cell Press, pp. 3845–3846, 2026.","mla":"Riedl, Michael, and Michael K. Sixt. “A New Sense for Electrical Fields.” <i>Cell</i>, vol. 189, no. 13, Cell Press, 2026, pp. 3845–46, doi:<a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">10.1016/j.cell.2026.05.038</a>.","ama":"Riedl M, Sixt MK. A new sense for electrical fields. <i>Cell</i>. 2026;189(13):3845-3846. doi:<a href=\"https://doi.org/10.1016/j.cell.2026.05.038\">10.1016/j.cell.2026.05.038</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","supplementarymaterial":"no","date_updated":"2026-10-20T09:00:16Z","author":[{"first_name":"Michael","last_name":"Riedl","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311"},{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"date_created":"2026-06-28T22:01:34Z","status":"public","_id":"22144","title":"A new sense for electrical fields","abstract":[{"lang":"eng","text":"Most cells polarize and migrate in response to electrical fields. In this issue of Cell, Belliveau et al. identify TMEM154/Galvanin, a receptor that serves as a cellular antenna to sense electrical gradients and guide migration toward the cathode."}],"article_type":"comment","oa_version":"None","type":"journal_article","OA_type":"closed access","language":[{"iso":"eng"}],"issue":"13","scopus_import":"1","publication":"Cell","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]}},{"day":"05","corr_author":"1","external_id":{"pmid":["41494523"]},"department":[{"_id":"AmDo"},{"_id":"SiHi"}],"month":"01","publisher":"Cell Press","volume":36,"doi":"10.1016/j.cub.2025.11.056","quality_controlled":"1","year":"2026","pmid":1,"intvolume":"        36","date_published":"2026-01-05T00:00:00Z","article_processing_charge":"No","fulldoi":"https://doi.org/10.1016/j.cub.2025.11.056","publication":"Current Biology","issue":"1","scopus_import":"1","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"OA_type":"closed access","language":[{"iso":"eng"}],"_id":"20972","title":"Neuroscience: What doesn’t kill you makes you stronger","date_created":"2026-01-11T23:01:33Z","status":"public","author":[{"id":"5d5f6ea4-ef9e-11f0-a10a-85e12a3552af","first_name":"Hakan","last_name":"Kücükdereli","full_name":"Kücükdereli, Hakan"},{"last_name":"Douglass","first_name":"Amelia May Barnett","id":"de5f6fda-80fb-11ef-996f-a8c4ecd8e289","full_name":"Douglass, Amelia May Barnett","orcid":"0000-0001-5398-6473"}],"article_type":"letter_note","oa_version":"None","type":"journal_article","abstract":[{"lang":"eng","text":"Small amounts of stress are thought to have beneficial effects. A new study reports a mechanism by which the psychedelic drug, psilocybin, causes acute release of stress hormones, despite its known long-term anti-anxiety effects."}],"citation":{"short":"H. Kücükdereli, A.M. Douglass, Current Biology 36 (2026) R27–R29.","ista":"Kücükdereli H, Douglass AM. 2026. Neuroscience: What doesn’t kill you makes you stronger. Current Biology. 36(1), R27–R29.","ieee":"H. Kücükdereli and A. M. Douglass, “Neuroscience: What doesn’t kill you makes you stronger,” <i>Current Biology</i>, vol. 36, no. 1. Cell Press, pp. R27–R29, 2026.","ama":"Kücükdereli H, Douglass AM. Neuroscience: What doesn’t kill you makes you stronger. <i>Current Biology</i>. 2026;36(1):R27-R29. doi:<a href=\"https://doi.org/10.1016/j.cub.2025.11.056\">10.1016/j.cub.2025.11.056</a>","mla":"Kücükdereli, Hakan, and Amelia M. Douglass. “Neuroscience: What Doesn’t Kill You Makes You Stronger.” <i>Current Biology</i>, vol. 36, no. 1, Cell Press, 2026, pp. R27–29, doi:<a href=\"https://doi.org/10.1016/j.cub.2025.11.056\">10.1016/j.cub.2025.11.056</a>.","apa":"Kücükdereli, H., &#38; Douglass, A. M. (2026). Neuroscience: What doesn’t kill you makes you stronger. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2025.11.056\">https://doi.org/10.1016/j.cub.2025.11.056</a>","chicago":"Kücükdereli, Hakan, and Amelia M. Douglass. “Neuroscience: What Doesn’t Kill You Makes You Stronger.” <i>Current Biology</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cub.2025.11.056\">https://doi.org/10.1016/j.cub.2025.11.056</a>."},"page":"R27-R29","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T08:57:26Z"},{"das_tickbox":"1","fulldoi":"https://doi.org/10.1016/j.devcel.2025.10.006","acknowledged_ssus":[{"_id":"NanoFab"}],"has_accepted_license":"1","date_published":"2026-02-11T00:00:00Z","file_date_updated":"2026-07-23T06:26:25Z","article_processing_charge":"Yes (in subscription journal)","keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"publication_status":"published","year":"2026","quality_controlled":"1","pmid":1,"intvolume":"        61","ddc":["570"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"volume":61,"researchdata_availability":"upon request","doi":"10.1016/j.devcel.2025.10.006","file":[{"access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"checksum":"52fd52d2d19a4514f8fcc1b40f420ca2","date_created":"2026-07-23T06:26:25Z","content_type":"application/pdf","date_updated":"2026-07-23T06:26:25Z","file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","file_size":12342817,"file_id":"22388"}],"external_id":{"pmid":["41192429"]},"publisher":"Cell Press","month":"02","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"day":"11","dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","oa":1,"supplementarymaterial":"yes","date_updated":"2026-10-20T09:01:14Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","PlanS_conform":"1","page":"356-371.e12","citation":{"mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, vol. 61, no. 2, Cell Press, 2026, p. 356–371.e12, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2026;61(2):356-371.e12. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12.","ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>, vol. 61, no. 2. Cell Press, p. 356–371.e12, 2026.","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>"},"type":"journal_article","oa_version":"Published Version","article_type":"original","abstract":[{"lang":"eng","text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo."}],"title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","_id":"20859","status":"public","author":[{"first_name":"Iván","last_name":"Company-Garrido","full_name":"Company-Garrido, Iván"},{"first_name":"Alberto","last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto"},{"full_name":"Colomer-Rosell, Mariona","last_name":"Colomer-Rosell","first_name":"Mariona"},{"first_name":"Bernard","last_name":"Ciraulo","full_name":"Ciraulo, Bernard"},{"first_name":"Ronja","last_name":"Molkenbur","full_name":"Molkenbur, Ronja"},{"first_name":"Peter","last_name":"Lanzerstorfer","full_name":"Lanzerstorfer, Peter"},{"full_name":"Pezzano, Fabio","last_name":"Pezzano","first_name":"Fabio"},{"last_name":"Agazzi","first_name":"Costanza","full_name":"Agazzi, Costanza"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert"},{"full_name":"Jain, Saumey","first_name":"Saumey","last_name":"Jain"},{"full_name":"Jacques, Jeroen M.","last_name":"Jacques","first_name":"Jeroen M."},{"full_name":"Venturini, Valeria","first_name":"Valeria","last_name":"Venturini"},{"full_name":"Knapp, Christian","last_name":"Knapp","first_name":"Christian"},{"full_name":"Xie, Yufei","last_name":"Xie","first_name":"Yufei"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"full_name":"Weghuber, Julian","first_name":"Julian","last_name":"Weghuber"},{"first_name":"Marcel","last_name":"Schaaf","full_name":"Schaaf, Marcel"},{"full_name":"Quidant, Romain","first_name":"Romain","last_name":"Quidant"},{"first_name":"Eva","last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva","orcid":"0000-0001-6165-5738"},{"last_name":"Ortega Arroyo","first_name":"Jaime","full_name":"Ortega Arroyo, Jaime"},{"full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stefan","last_name":"Wieser","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan"}],"date_created":"2025-12-28T23:01:27Z","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","publication":"Developmental Cell","scopus_import":"1","issue":"2"},{"page":"213-230.e7","citation":{"ieee":"R. Kelley <i>et al.</i>, “Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii,” <i>Molecular Cell</i>, vol. 86, no. 1. Cell Press, p. 213–230.e7, 2026.","short":"R. Kelley, S. Khavnekar, R.D. Righetto, J. Heebner, M. Obr, X. Zhang, S. Chakraborty, G. Tagiltsev, A.K. Michael, S. Van Dorst, F. Waltz, C.L. Mccafferty, L. Lamm, S. Zufferey, P. Van Der Stappen, H. Van Den Hoek, W. Wietrzynski, P. Harar, W. Wan, J.A.G. Briggs, J.M. Plitzko, B.D. Engel, A. Kotecha, Molecular Cell 86 (2026) 213–230.e7.","ista":"Kelley R, Khavnekar S, Righetto RD, Heebner J, Obr M, Zhang X, Chakraborty S, Tagiltsev G, Michael AK, Van Dorst S, Waltz F, Mccafferty CL, Lamm L, Zufferey S, Van Der Stappen P, Van Den Hoek H, Wietrzynski W, Harar P, Wan W, Briggs JAG, Plitzko JM, Engel BD, Kotecha A. 2026. Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii. Molecular Cell. 86(1), 213–230.e7.","ama":"Kelley R, Khavnekar S, Righetto RD, et al. Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii. <i>Molecular Cell</i>. 2026;86(1):213-230.e7. doi:<a href=\"https://doi.org/10.1016/j.molcel.2025.11.029\">10.1016/j.molcel.2025.11.029</a>","mla":"Kelley, Ron, et al. “Toward Community-Driven Visual Proteomics with Large-Scale Cryo-Electron Tomography of Chlamydomonas Reinhardtii.” <i>Molecular Cell</i>, vol. 86, no. 1, Cell Press, 2026, p. 213–230.e7, doi:<a href=\"https://doi.org/10.1016/j.molcel.2025.11.029\">10.1016/j.molcel.2025.11.029</a>.","apa":"Kelley, R., Khavnekar, S., Righetto, R. D., Heebner, J., Obr, M., Zhang, X., … Kotecha, A. (2026). Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii. <i>Molecular Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molcel.2025.11.029\">https://doi.org/10.1016/j.molcel.2025.11.029</a>","chicago":"Kelley, Ron, Sagar Khavnekar, Ricardo D. Righetto, Jessica Heebner, Martin Obr, Xianjun Zhang, Saikat Chakraborty, et al. “Toward Community-Driven Visual Proteomics with Large-Scale Cryo-Electron Tomography of Chlamydomonas Reinhardtii.” <i>Molecular Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.molcel.2025.11.029\">https://doi.org/10.1016/j.molcel.2025.11.029</a>."},"PlanS_conform":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-10-20T09:02:52Z","supplementarymaterial":"yes","status":"public","date_created":"2026-01-04T23:01:36Z","author":[{"first_name":"Ron","last_name":"Kelley","full_name":"Kelley, Ron"},{"last_name":"Khavnekar","first_name":"Sagar","full_name":"Khavnekar, Sagar"},{"full_name":"Righetto, Ricardo D.","first_name":"Ricardo D.","last_name":"Righetto"},{"full_name":"Heebner, Jessica","first_name":"Jessica","last_name":"Heebner"},{"id":"4741CA5A-F248-11E8-B48F-1D18A9856A87","last_name":"Obr","first_name":"Martin","full_name":"Obr, Martin","orcid":"0000-0003-1756-6564"},{"last_name":"Zhang","first_name":"Xianjun","full_name":"Zhang, Xianjun"},{"full_name":"Chakraborty, Saikat","first_name":"Saikat","last_name":"Chakraborty"},{"first_name":"Grigory","last_name":"Tagiltsev","full_name":"Tagiltsev, Grigory"},{"full_name":"Michael, Alicia","orcid":"0000-0002-6080-839X","last_name":"Michael","first_name":"Alicia","id":"6437c950-2a03-11ee-914d-d6476dd7b75c"},{"first_name":"Sofie","last_name":"Van Dorst","full_name":"Van Dorst, Sofie"},{"full_name":"Waltz, Florent","last_name":"Waltz","first_name":"Florent"},{"full_name":"Mccafferty, Caitlyn L.","first_name":"Caitlyn L.","last_name":"Mccafferty"},{"first_name":"Lorenz","last_name":"Lamm","full_name":"Lamm, Lorenz"},{"full_name":"Zufferey, Simon","first_name":"Simon","last_name":"Zufferey"},{"last_name":"Van Der Stappen","first_name":"Philippe","full_name":"Van Der Stappen, Philippe"},{"full_name":"Van Den Hoek, Hugo","last_name":"Van Den Hoek","first_name":"Hugo"},{"full_name":"Wietrzynski, Wojciech","last_name":"Wietrzynski","first_name":"Wojciech"},{"id":"e03d953a-6e8c-11ef-99e4-f0717d385cd5","first_name":"Pavol","last_name":"Harar","full_name":"Harar, Pavol","orcid":"0000-0001-5206-1794"},{"last_name":"Wan","first_name":"William","full_name":"Wan, William"},{"full_name":"Briggs, John A.G.","first_name":"John A.G.","last_name":"Briggs"},{"last_name":"Plitzko","first_name":"Jürgen M.","full_name":"Plitzko, Jürgen M."},{"full_name":"Engel, Benjamin D.","first_name":"Benjamin D.","last_name":"Engel"},{"first_name":"Abhay","last_name":"Kotecha","full_name":"Kotecha, Abhay"}],"_id":"20935","title":"Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii","abstract":[{"text":"In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate the structures of biomolecules in their native context. However, challenges remain for the efficient production and sharing of large-scale cryo-ET datasets. Here, we combined cryogenic plasma-based focused ion beam (cryo-PFIB) milling with recent advances in cryo-ET acquisition and processing to generate a dataset of 1,829 annotated tomograms of the green alga Chlamydomonas reinhardtii, which we provide as a community resource to drive method development and inspire biological discovery. To assay data quality, we performed subtomogram averaging of both soluble and membrane-bound complexes ranging in size from >3 MDa to ∼200 kDa, including 80S ribosomes, Rubisco, nucleosomes, microtubules, clathrin, photosystem II, and mitochondrial ATP synthase. The majority of these density maps reached sub-nanometer resolution, demonstrating the potential of this C. reinhardtii dataset as well as the promise of modern cryo-ET workflows and open data sharing to empower visual proteomics.","lang":"eng"}],"article_type":"original","oa_version":"Published Version","type":"journal_article","OA_place":"publisher","OA_type":"hybrid","language":[{"iso":"eng"}],"issue":"1","scopus_import":"1","publication":"Molecular Cell","acknowledgement":"Calculations were performed at the Max Planck Institute of Biochemistry and the Raven Supercomputer of the Max Planck Computing and Data Facility (MPCDF) in Garching, Germany; at the sciCORE (http://scicore.unibas.ch/) scientific computing center at the University of Basel, Switzerland; and at Thermo Fisher Scientific, in Eindhoven, the Netherlands. This work was supported by Thermo Fisher Scientific. All lamella preparations and tilt-series collections used in this work were conducted at Thermo Fisher R&D facilities in Brno and Eindhoven, utilizing Arctis and Krios microscopes. This work was also supported by the ERC consolidator grant “cryOcean” (fulfilled by the Swiss State Secretariat for Education, Research and Innovation, M822.00045) as well as a Swiss Nanoscience Institute PhD school grant to B.D.E. and P.V.d.S., an EMBO long-term postdoctoral fellowship (ALTF-383-2022) to G.T., an SNSF Postdoctoral Fellowship (project 210561) to F.W., a Boehringer Ingelheim Fonds fellowship to L.L., and by the Max Planck Society to J.A.G.B. and J.M.P.","publication_identifier":{"issn":["1097-2765"],"eissn":["1097-4164"]},"article_processing_charge":"Yes (in subscription journal)","date_published":"2026-01-08T00:00:00Z","file_date_updated":"2026-07-28T07:38:45Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1016/j.molcel.2025.11.029","das_tickbox":"1","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"intvolume":"        86","quality_controlled":"1","year":"2026","publication_status":"published","file":[{"access_level":"open_access","creator":"dernst","relation":"main_file","success":1,"date_created":"2026-07-28T07:38:45Z","checksum":"96a2f8519124d1a0d9de8d2594bf7147","content_type":"application/pdf","date_updated":"2026-07-28T07:38:45Z","file_name":"2026_MolecularCell_Kelley.pdf","file_size":26749637,"file_id":"22599"}],"doi":"10.1016/j.molcel.2025.11.029","researchdata_availability":"yes","volume":86,"oa":1,"dataavailabilitystatement":"Raw EM data are available at the EMPIAR under accession code EMPIAR: EMPIAR-11830. Annotation and processing information for all 1,829 tomograms are provided in spreadsheet format.153 The following subtomogram averages have been deposited at the Electron Microscopy Data Bank (EMDB): 80S ribosome (EMDB: EMD-51847), nucleosome (EMDB: EMD-19906), PSII (EMDB: EMD-51731), Rubisco (EMDB: EMD-51848), microtubule (EMDB: EMD-51804), clathrin (EMDB: EMD-51789), and ATP synthase (EMDB: EMD-51802). Segmentations shown in Figures 2 and 3 are deposited on Zenodo (https://doi.org/10.5281/zenodo.15875785). Particle positions and orientations used for STA, along with all resources derived from this work, are available on GitHub (https://github.com/Chromatin-Structure-Rhythms-Lab/ChlamyAnnotations). Reconstructed tomograms and annotations are also available to explore interactively at the CZII Cryo-ET Data Portal (DS-10302, https://cryoetdataportal.czscience.com/datasets/10302/). Raw data for cryo-PFIB/SEM slice-and-view of a whole C. reinhardtii cell has also been deposited (EMPIAR: EMPIAR-11275).\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","day":"08","month":"01","department":[{"_id":"AlMi"}],"publisher":"Cell Press"},{"ec_funded":1,"publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"acknowledgement":"We would like to thank J. Benichov and N. Hein for their help with fieldwork; M. Ramadas for helping with the segmentation analysis; T. Eliav, C. Chintaluri, G. Tkacik, and A. Navas for providing helpful comments to the project and manuscript; and A. Costalunga for the drawings of nightingales. Funding sources: The Joachim Herz Stiftung Add-on Fellowships for Interdisciplinary Life Science, awarded to G.C.; the ERC Consolidator Grant 819603 SYNAPSEEK, awarded to T.P.V.; and DFG Research Unit 5768–532521431, DFG Research Grant-547921981, DFG SFB 1315–327654276, and the ERC Starting Grant 757459 MIDNIGHT, awarded to D.V.","scopus_import":"1","issue":"3","publication":"Current Biology","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"hybrid","abstract":[{"lang":"eng","text":"During complex vocal interactions, different features of acoustic stimuli are integrated to produce appropriate vocal responses,1 such as copying sounds during vocal matching behavior in some animals.2,3,4,5,6,7,8,9,10,11,12 However, little is known about the interplay and possible trade-offs between the different temporal and spectral acoustic features during these vocal exchanges.2,13,14 Nightingales can flexibly match the pitch of their tonal “whistle songs” in real time during counter-singing duels.15,16 Here, we show that the syllable duration of whistle playbacks could alter the song responses of wild nightingales, causing their whistle duration distribution to shift toward the presented stimulus duration. When exposed to whistle playbacks featuring unnatural combinations of pitch and duration, nightingales demonstrate a flexible trade-off between pitch matching and temporal imitation, yet they are constrained by their vocal repertoire. They selectively adapted their vocal responses to approximate these novel stimuli, aligning them with their natural whistle repertoire. We developed a computational model of nightingale whistle-matching behavior that revealed a hierarchical organization of acoustic feature production. During whistle matching, the feature integration process is constrained by the duration of syllables, and pitch matching follows within this temporal framework, forcing a trade-off between the two features. Our findings reveal a complex interplay between the spectral and temporal domains that shapes song-matching behavior."}],"oa_version":"Published Version","type":"journal_article","article_type":"original","status":"public","author":[{"id":"1271b54b-dbcd-11ea-9d1d-d92da838fe2c","last_name":"Calderon Garcia","first_name":"Juan Sebastian","full_name":"Calderon Garcia, Juan Sebastian"},{"full_name":"Costalunga, Giacomo","last_name":"Costalunga","first_name":"Giacomo"},{"full_name":"Vogels, Tim P","orcid":"0000-0003-3295-6181","id":"CB6FF8D2-008F-11EA-8E08-2637E6697425","last_name":"Vogels","first_name":"Tim P"},{"full_name":"Vallentin, Daniela","first_name":"Daniela","last_name":"Vallentin"}],"date_created":"2026-01-14T12:00:29Z","title":"Interplay between syllable duration and pitch during whistle matching in wild nightingales","_id":"20986","PlanS_conform":"1","supplementarymaterial":"yes","date_updated":"2026-10-20T09:02:18Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Calderon Garcia, J. S., Costalunga, G., Vogels, T. P., &#38; Vallentin, D. (2026). Interplay between syllable duration and pitch during whistle matching in wild nightingales. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2025.12.025\">https://doi.org/10.1016/j.cub.2025.12.025</a>","chicago":"Calderon Garcia, Juan Sebastian, Giacomo Costalunga, Tim P Vogels, and Daniela Vallentin. “Interplay between Syllable Duration and Pitch during Whistle Matching in Wild Nightingales.” <i>Current Biology</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cub.2025.12.025\">https://doi.org/10.1016/j.cub.2025.12.025</a>.","short":"J.S. Calderon Garcia, G. Costalunga, T.P. Vogels, D. Vallentin, Current Biology 36 (2026) 791–798.e6.","ista":"Calderon Garcia JS, Costalunga G, Vogels TP, Vallentin D. 2026. Interplay between syllable duration and pitch during whistle matching in wild nightingales. Current Biology. 36(3), 791–798.e6.","ieee":"J. S. Calderon Garcia, G. Costalunga, T. P. Vogels, and D. Vallentin, “Interplay between syllable duration and pitch during whistle matching in wild nightingales,” <i>Current Biology</i>, vol. 36, no. 3. Cell Press, p. 791–798.e6, 2026.","mla":"Calderon Garcia, Juan Sebastian, et al. “Interplay between Syllable Duration and Pitch during Whistle Matching in Wild Nightingales.” <i>Current Biology</i>, vol. 36, no. 3, Cell Press, 2026, p. 791–798.e6, doi:<a href=\"https://doi.org/10.1016/j.cub.2025.12.025\">10.1016/j.cub.2025.12.025</a>.","ama":"Calderon Garcia JS, Costalunga G, Vogels TP, Vallentin D. Interplay between syllable duration and pitch during whistle matching in wild nightingales. <i>Current Biology</i>. 2026;36(3):791-798.e6. doi:<a href=\"https://doi.org/10.1016/j.cub.2025.12.025\">10.1016/j.cub.2025.12.025</a>"},"page":"791-798.e6","project":[{"call_identifier":"H2020","name":"Learning the shape of synaptic plasticity rules for neuronal architectures and function through machine learning.","_id":"0aacfa84-070f-11eb-9043-d7eb2c709234","grant_number":"819603"}],"publisher":"Cell Press","department":[{"_id":"GradSch"},{"_id":"TiVo"}],"month":"02","external_id":{"pmid":["41529680"]},"oa":1,"dataavailabilitystatement":"All data have been deposited at https://github.com/vallentinlab/NG-whistle-durations and are publicly available as of the date of publication.\r\nAll original code has been deposited at https://github.com/vallentinlab/NG-whistle-durations and is publicly available as of the date of publication.\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","day":"02","doi":"10.1016/j.cub.2025.12.025","researchdata_availability":"yes","file":[{"content_type":"application/pdf","date_updated":"2026-07-27T10:47:55Z","file_name":"2026_CurrentBiology_CalderonGarcia.pdf","file_size":7120959,"file_id":"22416","access_level":"open_access","creator":"dernst","relation":"main_file","success":1,"date_created":"2026-07-27T10:47:55Z","checksum":"e17c3537193d5ab4886596d1a04f9b0e"}],"volume":36,"intvolume":"        36","publication_status":"published","quality_controlled":"1","pmid":1,"year":"2026","ddc":["570","577"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"das_tickbox":"1","fulldoi":"https://doi.org/10.1016/j.cub.2025.12.025","article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","date_published":"2026-02-02T00:00:00Z","file_date_updated":"2026-07-27T10:47:55Z"},{"abstract":[{"lang":"eng","text":"The mammalian brain stores glucose, the main circulating energy substrate, as glycogen. In rodents, the cerebellum contains relatively high glycogen levels, yet its cellular and subcellular distribution remains poorly defined. Using monoclonal antibodies against glycogen, we examined its distribution in the mouse cerebellar cortex. Glycogen was predominantly localized to Bergmann glia (BG) processes in the molecular layer and was also detected in Purkinje cells (PCs), the principal cerebellar neurons. To assess the functional significance of cerebellar glycogen, we analyzed behavior in mice lacking glycogen synthase 1 (Gys1) in BG or PCs using a floxed Gys1 line. Gys1 deficiency in either PCs or GFAP-positive cells reduced anxiety-like behavior, whereas combined deletion caused PC degeneration and ataxia. These findings reveal a critical role for glycogen metabolism in both astrocytes and neurons in cerebellar function."}],"type":"journal_article","oa_version":"Published Version","article_type":"original","date_created":"2026-03-29T22:07:07Z","status":"public","author":[{"last_name":"Akther","first_name":"Sonam","full_name":"Akther, Sonam"},{"last_name":"Lee","first_name":"Ashley Bomin","full_name":"Lee, Ashley Bomin"},{"first_name":"Ayumu","last_name":"Konno","full_name":"Konno, Ayumu"},{"full_name":"Asiminas, Antonis","last_name":"Asiminas","first_name":"Antonis"},{"last_name":"Vittani","first_name":"Marta","full_name":"Vittani, Marta"},{"first_name":"Tsuneko","last_name":"Mishima","full_name":"Mishima, Tsuneko"},{"first_name":"Hirokazu","last_name":"Hirai","full_name":"Hirai, Hirokazu"},{"full_name":"Meehan, Claire Francesca","first_name":"Claire Francesca","last_name":"Meehan"},{"full_name":"Duran, Jordi","last_name":"Duran","first_name":"Jordi"},{"first_name":"Joan","last_name":"Guinovart","full_name":"Guinovart, Joan"},{"last_name":"Ashida","first_name":"Hitoshi","full_name":"Ashida, Hitoshi"},{"full_name":"Morita, Tsuyoshi","last_name":"Morita","first_name":"Tsuyoshi"},{"full_name":"Baba, Otto","last_name":"Baba","first_name":"Otto"},{"last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi"},{"first_name":"Maiken","last_name":"Nedergaard","full_name":"Nedergaard, Maiken"},{"last_name":"Hirase","first_name":"Hajime","full_name":"Hirase, Hajime"}],"title":"Distribution and functional significance of rodent cerebellar glycogen","_id":"21502","PlanS_conform":"1","supplementarymaterial":"no","date_updated":"2026-10-20T09:02:33Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Akther, S., Lee, A. B., Konno, A., Asiminas, A., Vittani, M., Mishima, T., … Hirase, H. (2026). Distribution and functional significance of rodent cerebellar glycogen. <i>IScience</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.isci.2026.115192\">https://doi.org/10.1016/j.isci.2026.115192</a>","chicago":"Akther, Sonam, Ashley Bomin Lee, Ayumu Konno, Antonis Asiminas, Marta Vittani, Tsuneko Mishima, Hirokazu Hirai, et al. “Distribution and Functional Significance of Rodent Cerebellar Glycogen.” <i>IScience</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.isci.2026.115192\">https://doi.org/10.1016/j.isci.2026.115192</a>.","ista":"Akther S, Lee AB, Konno A, Asiminas A, Vittani M, Mishima T, Hirai H, Meehan CF, Duran J, Guinovart J, Ashida H, Morita T, Baba O, Shigemoto R, Nedergaard M, Hirase H. 2026. Distribution and functional significance of rodent cerebellar glycogen. iScience. 29(4), 115192.","ieee":"S. Akther <i>et al.</i>, “Distribution and functional significance of rodent cerebellar glycogen,” <i>iScience</i>, vol. 29, no. 4. Cell Press, 2026.","short":"S. Akther, A.B. Lee, A. Konno, A. Asiminas, M. Vittani, T. Mishima, H. Hirai, C.F. Meehan, J. Duran, J. Guinovart, H. Ashida, T. Morita, O. Baba, R. Shigemoto, M. Nedergaard, H. Hirase, IScience 29 (2026).","mla":"Akther, Sonam, et al. “Distribution and Functional Significance of Rodent Cerebellar Glycogen.” <i>IScience</i>, vol. 29, no. 4, 115192, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.isci.2026.115192\">10.1016/j.isci.2026.115192</a>.","ama":"Akther S, Lee AB, Konno A, et al. Distribution and functional significance of rodent cerebellar glycogen. <i>iScience</i>. 2026;29(4). doi:<a href=\"https://doi.org/10.1016/j.isci.2026.115192\">10.1016/j.isci.2026.115192</a>"},"DOAJ_listed":"1","publication_identifier":{"eissn":["2589-0042"]},"acknowledgement":"This work was supported by the Novo Nordisk Foundation (NNFOC0058058, H. Hirase), the Danmarks Frie Forskningsfond (0134-00107B and 5283-00069A, H.Hirase), the Lundbeck Foundation, Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) program (22K06454/24H01221, A.K.; 23K27482, H.Hirai), the Japan Agency for Medical Research and Development (AMED) Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) (JP21dm0207111, H. Hirai), AMED Brain/MINDS 2.0 (JP23wm0625001 and JP24wm0625103, H. Hirai), and grants from the Spanish Ministerio de Ciencia e Innovación (MCIU/FEDER/AEI) (PID2020-118699 GB-100, J.D.) and the Fundación Ramón Areces (J.D.). Sonam Akther has been supported by the RIKEN IPA fellowship. We are thankful to Dr. Yuki Oe for his support in the initial stage of this study and to Dan Xue for his help with the graphical abstract. We thank Dr. Pia Weikop for providing CTN research infrastructure. The authors declare no competing financial interests.","scopus_import":"1","issue":"4","publication":"iScience","language":[{"iso":"eng"}],"OA_place":"publisher","OA_type":"gold","intvolume":"        29","publication_status":"published","pmid":1,"year":"2026","quality_controlled":"1","ddc":["570"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"das_tickbox":"1","fulldoi":"https://doi.org/10.1016/j.isci.2026.115192","article_processing_charge":"Yes","has_accepted_license":"1","file_date_updated":"2026-07-27T12:03:23Z","date_published":"2026-04-17T00:00:00Z","publisher":"Cell Press","month":"04","department":[{"_id":"RySh"}],"external_id":{"pmid":["41890976"]},"oa":1,"dataavailabilitystatement":"This study did not generate new materials or reagents.All data supporting the findings of this study are available from the lead contact upon reasonable request.\r\nThis study did not generate any new code.\r\nAdditional raw data can be obtained from the lead contact upon reasonable request.","day":"17","doi":"10.1016/j.isci.2026.115192","researchdata_availability":"upon request","file":[{"success":1,"checksum":"9c9ccc87072d04808e4af407d647ed20","date_created":"2026-07-27T12:03:23Z","access_level":"open_access","relation":"main_file","creator":"dernst","file_id":"22427","file_name":"2026_iScience_Akther.pdf","date_updated":"2026-07-27T12:03:23Z","content_type":"application/pdf","file_size":14469960}],"volume":29,"article_number":"115192"},{"title":"Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination","_id":"20924","status":"public","date_created":"2026-01-04T23:01:33Z","author":[{"first_name":"Wataru","last_name":"Kobayashi","full_name":"Kobayashi, Wataru"},{"full_name":"Michael, Alicia","orcid":"0000-0002-6080-839X","id":"6437c950-2a03-11ee-914d-d6476dd7b75c","first_name":"Alicia","last_name":"Michael"},{"full_name":"Ruangroengkulrith, Siwat","last_name":"Ruangroengkulrith","first_name":"Siwat"},{"full_name":"Kümmecke, Maximilian","first_name":"Maximilian","last_name":"Kümmecke"},{"full_name":"Tachibana, Kikuë","last_name":"Tachibana","first_name":"Kikuë"}],"oa_version":"Published Version","type":"journal_article","article_type":"original","abstract":[{"text":"Pioneer transcription factors (TFs) possess the ability to read out DNA motifs embedded within nucleosomes, driving changes in gene expression during cellular differentiation and reprogramming. Here, we present selected engagement on nucleosome sequencing (SeEN-seq), a protocol designed to systematically identify potential TF-binding sites on the nucleosome. We describe steps for nucleosome library assembly, SeEN-seq assay, and cryoelectron microscopy (cryo-EM) sample preparation. This protocol facilitates the preparation of homogeneous pioneer TF-nucleosome complexes for cryo-EM structure determination using single-particle analysis.\r\nFor complete details on the use and execution of this protocol, please refer to Michael et al.1","lang":"eng"}],"DOAJ_listed":"1","citation":{"apa":"Kobayashi, W., Michael, A. K., Ruangroengkulrith, S., Kümmecke, M., &#38; Tachibana, K. (2026). Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination. <i>STAR Protocols</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.xpro.2025.104295\">https://doi.org/10.1016/j.xpro.2025.104295</a>","chicago":"Kobayashi, Wataru, Alicia K. Michael, Siwat Ruangroengkulrith, Maximilian Kümmecke, and Kikuë Tachibana. “Protocol for Integrative Analysis of Transcription Factor-Nucleosome Interactions Using SeEN-Seq and Cryo-EM Structure Determination.” <i>STAR Protocols</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.xpro.2025.104295\">https://doi.org/10.1016/j.xpro.2025.104295</a>.","short":"W. Kobayashi, A.K. Michael, S. Ruangroengkulrith, M. Kümmecke, K. Tachibana, STAR Protocols 7 (2026).","ista":"Kobayashi W, Michael AK, Ruangroengkulrith S, Kümmecke M, Tachibana K. 2026. Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination. STAR Protocols. 7(1), 104295.","ieee":"W. Kobayashi, A. K. Michael, S. Ruangroengkulrith, M. Kümmecke, and K. Tachibana, “Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination,” <i>STAR Protocols</i>, vol. 7, no. 1. Cell Press, 2026.","mla":"Kobayashi, Wataru, et al. “Protocol for Integrative Analysis of Transcription Factor-Nucleosome Interactions Using SeEN-Seq and Cryo-EM Structure Determination.” <i>STAR Protocols</i>, vol. 7, no. 1, 104295, Cell Press, 2026, doi:<a href=\"https://doi.org/10.1016/j.xpro.2025.104295\">10.1016/j.xpro.2025.104295</a>.","ama":"Kobayashi W, Michael AK, Ruangroengkulrith S, Kümmecke M, Tachibana K. Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination. <i>STAR Protocols</i>. 2026;7(1). doi:<a href=\"https://doi.org/10.1016/j.xpro.2025.104295\">10.1016/j.xpro.2025.104295</a>"},"project":[{"grant_number":"101162145","name":"Circadian structural transitions of chromatin","_id":"9136c684-16d5-11f0-9cad-91c0177b365f"}],"supplementarymaterial":"yes","date_updated":"2026-10-20T09:01:26Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","PlanS_conform":"1","publication":"STAR Protocols","scopus_import":"1","issue":"1","acknowledgement":"We thank R.H. Kim, A. Casper, and R. Gautsch for sequencing at the NGS facility (RRID:SCR_025746). K.T. is an Honorary Professor at the Department of Biology, Ludwig-Maximilians-University, Munich, Germany. This study was funded by European Research Council grant ERC-CoG-818556 TotipotentZygotChrom (K.T.), Max Planck Society (K.T.), and ERC Starting Grant “ChromaChrono” 101162145 (A.K.M.).","publication_identifier":{"eissn":["2666-1667"]},"OA_place":"publisher","OA_type":"gold","language":[{"iso":"eng"}],"ddc":["570"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publication_status":"published","pmid":1,"quality_controlled":"1","year":"2026","intvolume":"         7","has_accepted_license":"1","date_published":"2026-03-20T00:00:00Z","file_date_updated":"2026-07-23T06:33:24Z","article_processing_charge":"Yes","das_tickbox":"1","fulldoi":"https://doi.org/10.1016/j.xpro.2025.104295","dataavailabilitystatement":"Raw SeEN-seq data of ESRRB nucleosome binding have been deposited on the Sequence Read Achieve database under the accession PRJNA1305216. Example analysis scripts and input files for SeEN-seq analysis can be found at https://doi.org/10.5281/zenodo.17665082.","day":"20","oa":1,"corr_author":"1","external_id":{"pmid":["41455105"]},"publisher":"Cell Press","department":[{"_id":"AlMi"}],"month":"03","article_number":"104295","volume":7,"researchdata_availability":"yes","doi":"10.1016/j.xpro.2025.104295","file":[{"relation":"main_file","creator":"dernst","access_level":"open_access","checksum":"cf04b061a48548a649e6a2435bf120db","date_created":"2026-07-23T06:33:24Z","success":1,"file_size":5531906,"date_updated":"2026-07-23T06:33:24Z","content_type":"application/pdf","file_name":"2026_StarProtocols_Kobayashi.pdf","file_id":"22389"}]},{"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"intvolume":"        31","publication_status":"published","quality_controlled":"1","year":"2026","pmid":1,"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","date_published":"2026-02-01T00:00:00Z","file_date_updated":"2026-07-27T08:26:40Z","das_tickbox":"0","fulldoi":"https://doi.org/10.1016/j.tplants.2025.10.018","oa":1,"corr_author":"1","day":"01","publisher":"Cell Press","department":[{"_id":"JiFr"}],"month":"02","external_id":{"pmid":["41249070"]},"researchdata_availability":"no","doi":"10.1016/j.tplants.2025.10.018","file":[{"file_id":"22413","file_size":432792,"date_updated":"2026-07-27T08:26:40Z","content_type":"application/pdf","file_name":"2026_TrendsPlantScience_Friml.pdf","date_created":"2026-07-27T08:26:40Z","checksum":"e60e903fb4b3e917dba763976ec652cd","success":1,"creator":"dernst","relation":"main_file","access_level":"open_access"}],"volume":31,"author":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"status":"public","date_created":"2025-12-02T16:29:22Z","title":"Role of cAMP in TIR1/AFB auxin signaling: Open issues","_id":"20725","abstract":[{"text":"The canonical mechanism by which the phytohormone auxin regulates transcription has been one of the cornerstones of plant signaling. The recent unexpected discovery of cyclic AMP (cAMP) as a second messenger in this pathway has revised its foundations while leaving many open questions and gaps in our understanding; these will be discussed in this forum article.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"review","citation":{"ista":"Friml J. 2026. Role of cAMP in TIR1/AFB auxin signaling: Open issues. Trends in Plant Science. 31(2), 136–138.","ieee":"J. Friml, “Role of cAMP in TIR1/AFB auxin signaling: Open issues,” <i>Trends in Plant Science</i>, vol. 31, no. 2. Cell Press, pp. 136–138, 2026.","short":"J. Friml, Trends in Plant Science 31 (2026) 136–138.","ama":"Friml J. Role of cAMP in TIR1/AFB auxin signaling: Open issues. <i>Trends in Plant Science</i>. 2026;31(2):136-138. doi:<a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">10.1016/j.tplants.2025.10.018</a>","mla":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” <i>Trends in Plant Science</i>, vol. 31, no. 2, Cell Press, 2026, pp. 136–38, doi:<a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">10.1016/j.tplants.2025.10.018</a>.","apa":"Friml, J. (2026). Role of cAMP in TIR1/AFB auxin signaling: Open issues. <i>Trends in Plant Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">https://doi.org/10.1016/j.tplants.2025.10.018</a>","chicago":"Friml, Jiří. “Role of CAMP in TIR1/AFB Auxin Signaling: Open Issues.” <i>Trends in Plant Science</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.tplants.2025.10.018\">https://doi.org/10.1016/j.tplants.2025.10.018</a>."},"page":"136-138","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"PlanS_conform":"1","supplementarymaterial":"no","date_updated":"2026-10-20T09:01:46Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","issue":"2","publication":"Trends in Plant Science","acknowledgement":"I apologize to colleagues whose relevant work I was unable to cite due to space limitations. This work was funded by the European Union (ERC, CYNIPS, 101142681) and Austrian Science Fund (FWF; 37051-B). I thank Drs Huihuang Chen, Yuanrong Pei, Jason Reed, Linlin Qi, and Dolf Weijers for inspiration and critical input.","publication_identifier":{"issn":["1360-1385"],"eissn":["1878-4372"]},"OA_place":"publisher","OA_type":"hybrid","language":[{"iso":"eng"}]},{"title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","_id":"20636","date_created":"2025-11-12T10:03:39Z","status":"public","author":[{"first_name":"R","last_name":"Huang","full_name":"Huang, R"},{"last_name":"Wang","first_name":"J","full_name":"Wang, J"},{"first_name":"M","last_name":"Chang","full_name":"Chang, M"},{"full_name":"Tang, W","first_name":"W","last_name":"Tang"},{"full_name":"Yu, Y","last_name":"Yu","first_name":"Y"},{"full_name":"Zhang, Y","first_name":"Y","last_name":"Zhang"},{"first_name":"Y","last_name":"Peng","full_name":"Peng, Y"},{"full_name":"Wang, Y","first_name":"Y","last_name":"Wang"},{"first_name":"Y","last_name":"Guo","full_name":"Guo, Y"},{"first_name":"T","last_name":"Lu","full_name":"Lu, T"},{"last_name":"Cao","first_name":"Y","full_name":"Cao, Y"},{"last_name":"Zhou","first_name":"Y","full_name":"Zhou, Y"},{"last_name":"Zhang","first_name":"Q","full_name":"Zhang, Q"},{"last_name":"Huang","first_name":"Y","full_name":"Huang, Y"},{"full_name":"Wu, A","first_name":"A","last_name":"Wu"},{"first_name":"L","last_name":"Ren","full_name":"Ren, L"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","last_name":"Gallei","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C"},{"full_name":"Dong, J","last_name":"Dong","first_name":"J"},{"first_name":"H","last_name":"Chen","full_name":"Chen, H"},{"first_name":"J","last_name":"He","full_name":"He, J"},{"first_name":"M","last_name":"Wen","full_name":"Wen, M"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sun, L","last_name":"Sun","first_name":"L"},{"full_name":"Xiong, Y","first_name":"Y","last_name":"Xiong"},{"first_name":"Z","last_name":"Yang","full_name":"Yang, Z"},{"first_name":"T","last_name":"Xu","full_name":"Xu, T"}],"oa_version":"None","type":"journal_article","article_type":"original","abstract":[{"lang":"eng","text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis."}],"page":"73-84","citation":{"mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>, vol. 61, no. 1, Cell Press, 2026, pp. 73–84, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>.","ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. 2026;61(1):73-84. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>","ieee":"R. Huang <i>et al.</i>, “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” <i>Developmental Cell</i>, vol. 61, no. 1. Cell Press, pp. 73–84, 2026.","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2026. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 61(1), 73–84.","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell 61 (2026) 73–84.","chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>.","apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2026). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>"},"date_updated":"2026-10-20T09:02:01Z","supplementarymaterial":"yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication":"Developmental Cell","scopus_import":"1","issue":"1","acknowledgement":"We thank Lukáš Fiedler‬ for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"OA_type":"closed access","language":[{"iso":"eng"}],"publication_status":"published","pmid":1,"year":"2026","quality_controlled":"1","intvolume":"        61","date_published":"2026-01-14T00:00:00Z","article_processing_charge":"No","das_tickbox":"1","fulldoi":"https://doi.org/10.1016/j.devcel.2025.09.009","day":"14","dataavailabilitystatement":"Plasmids and genetic materials generated in this study will be made available upon request from the lead contact.The mass spectrometry data reported in this work have been deposited at the iProX database. Original western blot gel images have been deposited to Mendeley Data. The accession code and the DOI are listed in the key resources table.\r\nThis paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","external_id":{"pmid":["41043435"]},"publisher":"Cell Press","month":"01","department":[{"_id":"JiFr"}],"volume":61,"researchdata_availability":"upon request","doi":"10.1016/j.devcel.2025.09.009"},{"publication":"Current Biology","scopus_import":"1","issue":"13","publication_identifier":{"issn":["0960-9822"]},"OA_type":"closed access","language":[{"iso":"eng"}],"title":"Auxin and the control of plant growth and development","_id":"22286","date_created":"2026-07-13T09:39:40Z","status":"public","author":[{"full_name":"Leitner, Valentin","id":"4c665ce3-0016-11ec-bea0-e44de7a4fa3d","first_name":"Valentin","last_name":"Leitner"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"}],"type":"journal_article","oa_version":"None","article_type":"original","abstract":[{"lang":"eng","text":"Plants are remarkable organisms. Unlike animals, they cannot flee and, rooted in one place, they must cope with whatever challenges arise — nutrient scarcity, drought, shade, wind or obstacles in the soil. Their extraordinary ability to survive in such unstable environmental conditions lies in their capacity to adapt. In response to environmental signals, plants can rapidly adjust the rate of organ growth, change the direction of growth, bend toward resources, or remodel their body architecture by promoting or suppressing the formation of new organs such as lateral roots, branches, leaves, or flowers. This unique developmental plasticity depends on chemical signals, plant hormones that serve as regulators and coordinators of endogenous molecular and cellular processes. Chief among these signals is auxin, a plant hormone central to nearly every aspect of plant life."}],"page":"R739-R744","citation":{"apa":"Leitner, V., &#38; Benková, E. (2026). Auxin and the control of plant growth and development. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">https://doi.org/10.1016/j.cub.2026.04.047</a>","chicago":"Leitner, Valentin, and Eva Benková. “Auxin and the Control of Plant Growth and Development.” <i>Current Biology</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">https://doi.org/10.1016/j.cub.2026.04.047</a>.","short":"V. Leitner, E. Benková, Current Biology 36 (2026) R739–R744.","ista":"Leitner V, Benková E. 2026. Auxin and the control of plant growth and development. Current Biology. 36(13), R739–R744.","ieee":"V. Leitner and E. Benková, “Auxin and the control of plant growth and development,” <i>Current Biology</i>, vol. 36, no. 13. Cell Press, pp. R739–R744, 2026.","mla":"Leitner, Valentin, and Eva Benková. “Auxin and the Control of Plant Growth and Development.” <i>Current Biology</i>, vol. 36, no. 13, Cell Press, 2026, pp. R739–44, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">10.1016/j.cub.2026.04.047</a>.","ama":"Leitner V, Benková E. Auxin and the control of plant growth and development. <i>Current Biology</i>. 2026;36(13):R739-R744. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">10.1016/j.cub.2026.04.047</a>"},"supplementarymaterial":"no","date_updated":"2026-10-20T09:00:35Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"06","corr_author":"1","external_id":{"pmid":["42407441"]},"publisher":"Cell Press","department":[{"_id":"EvBe"},{"_id":"GradSch"}],"month":"07","volume":36,"researchdata_availability":"no","doi":"10.1016/j.cub.2026.04.047","publication_status":"published","quality_controlled":"1","pmid":1,"year":"2026","intvolume":"        36","date_published":"2026-07-06T00:00:00Z","article_processing_charge":"No","das_tickbox":"0","fulldoi":"https://doi.org/10.1016/j.cub.2026.04.047"},{"volume":114,"researchdata_availability":"no","doi":"10.1016/j.neuron.2026.04.002","day":"20","corr_author":"1","external_id":{"pmid":["42161246"]},"department":[{"_id":"PeJo"}],"month":"05","publisher":"Cell Press","date_published":"2026-05-20T00:00:00Z","article_processing_charge":"No","fulldoi":"https://doi.org/10.1016/j.neuron.2026.04.002","das_tickbox":"0","year":"2026","pmid":1,"quality_controlled":"1","publication_status":"published","intvolume":"       114","OA_type":"closed access","language":[{"iso":"eng"}],"publication":"Neuron","issue":"10","scopus_import":"1","publication_identifier":{"issn":["0896-6273"]},"page":"1706-1708","citation":{"apa":"Bhattacharya, S., &#38; Jonas, P. M. (2026). Mission impossible? Quantitative analysis of dendritic computations in vivo. <i>Neuron</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.neuron.2026.04.002\">https://doi.org/10.1016/j.neuron.2026.04.002</a>","chicago":"Bhattacharya, Subhodeep, and Peter M Jonas. “Mission Impossible? Quantitative Analysis of Dendritic Computations in Vivo.” <i>Neuron</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.neuron.2026.04.002\">https://doi.org/10.1016/j.neuron.2026.04.002</a>.","short":"S. Bhattacharya, P.M. Jonas, Neuron 114 (2026) 1706–1708.","ista":"Bhattacharya S, Jonas PM. 2026. Mission impossible? Quantitative analysis of dendritic computations in vivo. Neuron. 114(10), 1706–1708.","ieee":"S. Bhattacharya and P. M. Jonas, “Mission impossible? Quantitative analysis of dendritic computations in vivo,” <i>Neuron</i>, vol. 114, no. 10. Cell Press, pp. 1706–1708, 2026.","mla":"Bhattacharya, Subhodeep, and Peter M. Jonas. “Mission Impossible? Quantitative Analysis of Dendritic Computations in Vivo.” <i>Neuron</i>, vol. 114, no. 10, Cell Press, 2026, pp. 1706–08, doi:<a href=\"https://doi.org/10.1016/j.neuron.2026.04.002\">10.1016/j.neuron.2026.04.002</a>.","ama":"Bhattacharya S, Jonas PM. Mission impossible? Quantitative analysis of dendritic computations in vivo. <i>Neuron</i>. 2026;114(10):1706-1708. doi:<a href=\"https://doi.org/10.1016/j.neuron.2026.04.002\">10.1016/j.neuron.2026.04.002</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","supplementarymaterial":"no","date_updated":"2026-10-20T09:00:56Z","_id":"22305","title":"Mission impossible? Quantitative analysis of dendritic computations in vivo","date_created":"2026-07-13T10:51:27Z","author":[{"orcid":"0009-0009-0334-9068","full_name":"Bhattacharya, Subhodeep","first_name":"Subhodeep","last_name":"Bhattacharya","id":"45f74010-66f6-11f0-a7d4-c441fa3685ff"},{"full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","first_name":"Peter M","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"status":"public","article_type":"letter_note","oa_version":"None","type":"journal_article","abstract":[{"text":"Active dendrites enrich the repertoire of single-neuron computations. Whereas a lot is known about the function of dendrites in vitro, information about their in vivo properties is limited. A new study1 uses advanced imaging to study hippocampal CA1 pyramidal neuron dendrites in head-fixed, awake animals.","lang":"eng"}]}]